LOCUS       DACCVU010000001        68941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCVU010000001 DACCVU010000000
VERSION     DACCVU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.001"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            153..1211
                     /locus_tag="IVW29_000001"
     CDS             153..1211
                     /locus_tag="IVW29_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7837515.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1230..2249
                     /locus_tag="IVW29_000002"
     CDS             1230..2249
                     /locus_tag="IVW29_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7837516.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2351..3067)
                     /locus_tag="IVW29_000003"
     CDS             complement(2351..3067)
                     /locus_tag="IVW29_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7837517.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3036..3821)
                     /locus_tag="IVW29_000004"
     CDS             complement(3036..3821)
                     /locus_tag="IVW29_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7837518.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3812..4561)
                     /locus_tag="IVW29_000005"
     CDS             complement(3812..4561)
                     /locus_tag="IVW29_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7837519.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4859..5383
                     /locus_tag="IVW29_000006"
     CDS             4859..5383
                     /locus_tag="IVW29_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837520.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5399..6898
                     /locus_tag="IVW29_000007"
     CDS             5399..6898
                     /locus_tag="IVW29_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7837521.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6986..7588)
                     /locus_tag="IVW29_000008"
     CDS             complement(6986..7588)
                     /locus_tag="IVW29_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7837522.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7692..8648
                     /locus_tag="IVW29_000009"
     CDS             7692..8648
                     /locus_tag="IVW29_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7837523.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8953..9909)
                     /locus_tag="IVW29_000010"
     CDS             complement(8953..9909)
                     /locus_tag="IVW29_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7837524.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10083..10643
                     /locus_tag="IVW29_000011"
     CDS             10083..10643
                     /locus_tag="IVW29_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837525.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10636..11460
                     /locus_tag="IVW29_000012"
     CDS             10636..11460
                     /locus_tag="IVW29_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7837526.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11470..12087
                     /locus_tag="IVW29_000013"
     CDS             11470..12087
                     /locus_tag="IVW29_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7837527.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12105..12788
                     /locus_tag="IVW29_000014"
     CDS             12105..12788
                     /locus_tag="IVW29_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7837528.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13471..14754
                     /locus_tag="IVW29_000015"
     CDS             13471..14754
                     /locus_tag="IVW29_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7837529.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14770..15657
                     /locus_tag="IVW29_000016"
     CDS             14770..15657
                     /locus_tag="IVW29_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7837530.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15674..16510
                     /locus_tag="IVW29_000017"
     CDS             15674..16510
                     /locus_tag="IVW29_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7837531.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16513..17136
                     /locus_tag="IVW29_000018"
     CDS             16513..17136
                     /locus_tag="IVW29_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837532.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17133..18425
                     /locus_tag="IVW29_000019"
     CDS             17133..18425
                     /locus_tag="IVW29_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7837533.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18784..18881
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18997..19578
                     /locus_tag="IVW29_000020"
     CDS             18997..19578
                     /locus_tag="IVW29_000020"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7837534.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19581..20888
                     /locus_tag="IVW29_000021"
     CDS             19581..20888
                     /locus_tag="IVW29_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7837535.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21304..21792
                     /gene="purE"
                     /locus_tag="IVW29_000022"
     CDS             21304..21792
                     /gene="purE"
                     /locus_tag="IVW29_000022"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7837536.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21776..22909
                     /gene="purK"
                     /locus_tag="IVW29_000023"
     CDS             21776..22909
                     /gene="purK"
                     /locus_tag="IVW29_000023"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7837537.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22922..24217
                     /gene="purB"
                     /locus_tag="IVW29_000024"
     CDS             22922..24217
                     /gene="purB"
                     /locus_tag="IVW29_000024"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7837538.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24339..25727)
                     /gene="ltrA"
                     /locus_tag="IVW29_000025"
     CDS             complement(24339..25727)
                     /gene="ltrA"
                     /locus_tag="IVW29_000025"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7837539.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26296..26673)
                     /locus_tag="IVW29_000026"
     CDS             complement(26296..26673)
                     /locus_tag="IVW29_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837540.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26929..27657
                     /locus_tag="IVW29_000027"
     CDS             26929..27657
                     /locus_tag="IVW29_000027"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7837541.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27657..27911
                     /gene="purS"
                     /locus_tag="IVW29_000028"
     CDS             27657..27911
                     /gene="purS"
                     /locus_tag="IVW29_000028"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7837542.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27913..28584
                     /gene="purQ"
                     /locus_tag="IVW29_000029"
     CDS             27913..28584
                     /gene="purQ"
                     /locus_tag="IVW29_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7837543.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28585..30807
                     /gene="purL"
                     /locus_tag="IVW29_000030"
     CDS             28585..30807
                     /gene="purL"
                     /locus_tag="IVW29_000030"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7837544.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30792..32231
                     /locus_tag="IVW29_000031"
     CDS             30792..32231
                     /locus_tag="IVW29_000031"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7837545.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32263..33306
                     /locus_tag="IVW29_000032"
     CDS             32263..33306
                     /locus_tag="IVW29_000032"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7837546.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            33303..33881
                     /gene="purN"
                     /locus_tag="IVW29_000033"
     CDS             33303..33881
                     /gene="purN"
                     /locus_tag="IVW29_000033"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7837547.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33878..35413
                     /gene="purH"
                     /locus_tag="IVW29_000034"
     CDS             33878..35413
                     /gene="purH"
                     /locus_tag="IVW29_000034"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7837548.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35429..36703
                     /gene="purD"
                     /locus_tag="IVW29_000035"
     CDS             35429..36703
                     /gene="purD"
                     /locus_tag="IVW29_000035"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7837549.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36825..37739
                     /locus_tag="IVW29_000036"
     CDS             36825..37739
                     /locus_tag="IVW29_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7837550.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37872..39437)
                     /locus_tag="IVW29_000037"
     CDS             complement(37872..39437)
                     /locus_tag="IVW29_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7837551.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39430..40881)
                     /locus_tag="IVW29_000038"
     CDS             complement(39430..40881)
                     /locus_tag="IVW29_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7837552.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40895..41863)
                     /locus_tag="IVW29_000039"
     CDS             complement(40895..41863)
                     /locus_tag="IVW29_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7837553.1"
                     /translation="MMGCTPNTKEAPDPLVVGFSQSGTESSWRKKHTESIITALEKED
                     YQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDSVLKEAKRAGIPVILVD
                     RHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEIRILELAGSENSTPTKL
                     RSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKGKDQMKRYLETNDLSEI
                     DVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIEKLRSGIVNCVVECKPD
                     VGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIPTRNY"
     gene            42130..43218
                     /locus_tag="IVW29_000040"
     CDS             42130..43218
                     /locus_tag="IVW29_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7837554.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43272..44798
                     /locus_tag="IVW29_000041"
     CDS             43272..44798
                     /locus_tag="IVW29_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7837555.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44800..46005
                     /locus_tag="IVW29_000042"
     CDS             44800..46005
                     /locus_tag="IVW29_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7837556.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46262..47239
                     /gene="guaC"
                     /locus_tag="IVW29_000043"
     CDS             46262..47239
                     /gene="guaC"
                     /locus_tag="IVW29_000043"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7837557.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47503..48927
                     /locus_tag="IVW29_000044"
     CDS             47503..48927
                     /locus_tag="IVW29_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7837558.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49388..50497
                     /gene="pdhA"
                     /locus_tag="IVW29_000045"
     CDS             49388..50497
                     /gene="pdhA"
                     /locus_tag="IVW29_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7837559.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50500..51477
                     /locus_tag="IVW29_000046"
     CDS             50500..51477
                     /locus_tag="IVW29_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7837560.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51502..53145
                     /locus_tag="IVW29_000047"
     CDS             51502..53145
                     /locus_tag="IVW29_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7837561.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53153..54559
                     /gene="lpdA"
                     /locus_tag="IVW29_000048"
     CDS             53153..54559
                     /gene="lpdA"
                     /locus_tag="IVW29_000048"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7837562.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54721..54996
                     /locus_tag="IVW29_000049"
     CDS             54721..54996
                     /locus_tag="IVW29_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7837563.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            55002..55769
                     /locus_tag="IVW29_000050"
     CDS             55002..55769
                     /locus_tag="IVW29_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7837564.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55937..57766
                     /gene="typA"
                     /locus_tag="IVW29_000051"
     CDS             55937..57766
                     /gene="typA"
                     /locus_tag="IVW29_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7837565.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            58027..59799
                     /gene="recQ"
                     /locus_tag="IVW29_000052"
     CDS             58027..59799
                     /gene="recQ"
                     /locus_tag="IVW29_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7837566.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59913..60215
                     /locus_tag="IVW29_000053"
     CDS             59913..60215
                     /locus_tag="IVW29_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7837567.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60218..61381
                     /locus_tag="IVW29_000054"
     CDS             60218..61381
                     /locus_tag="IVW29_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7837568.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61407..64835
                     /locus_tag="IVW29_000055"
     CDS             61407..64835
                     /locus_tag="IVW29_000055"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7837569.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64856..65980
                     /locus_tag="IVW29_000056"
     CDS             64856..65980
                     /locus_tag="IVW29_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837570.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65970..66407
                     /locus_tag="IVW29_000057"
     CDS             65970..66407
                     /locus_tag="IVW29_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7837571.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66391..66729
                     /locus_tag="IVW29_000058"
     CDS             66391..66729
                     /locus_tag="IVW29_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7837572.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66848..67408
                     /gene="rsmD"
                     /locus_tag="IVW29_000059"
     CDS             66848..67408
                     /gene="rsmD"
                     /locus_tag="IVW29_000059"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7837573.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67405..67896
                     /gene="coaD"
                     /locus_tag="IVW29_000060"
     CDS             67405..67896
                     /gene="coaD"
                     /locus_tag="IVW29_000060"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7837574.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67886..68929
                     /locus_tag="IVW29_000061"
     CDS             67886..68929
                     /locus_tag="IVW29_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7837575.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        22171 a        12015 c        15129 g        19626 t
ORIGIN      
        1 aaaaatcact ttacataagt ttcaaaagtt tctttaattt cctattgttc aataagtaac
       61 aatgtaaaga taaaattaaa aatgttaata cgtattagca aaataaaagc tttttttatt
      121 gtgaataaat gaacataatt aggaggagaa agatgaactt atttgacatt tttcgatcga
      181 ctttgacaga tatgtcgatt gtcagtgcaa tcacttcaac agtttttatt atattattag
      241 ggtttgtttg tagaaaaaag gggatttttt ctgaatctgt tggaaaaatt ctatcgaaag
      301 tcgtcttaac agtggctttg ccagcactgg catttcgagc tttcatgcaa gatatcaata
      361 cagaattact gatgcaagga atcaatgtct tgatttgggg attagttatt tatattattc
      421 ttattctagt ttcaaaacct tttttcttaa gctataaagg ggataagcaa acaacgttgc
      481 gtgtcctgac gatctttggt tctacgacct ttttcggaac gcctatcgtc agtgcgattt
      541 atggtcctgt cggcgtgatg tattcctcaa ttttcaatat tggctaccga atctttttgt
      601 attcttatgg atacataaaa atgagtggct tgaagatgga agctaaaaat atcaaagcga
      661 tgttcgtcaa tccaatcatt atcgctactt ttgccggcct tttcatttgg ctgttccaag
      721 ggtatctgcc acaagtgtca gttacaggtg cagaaggaga gataagccaa gtagcgtttt
      781 tgcgaattga ccagactgca ccgtggctat ttcagccgat ggattattta gctggtttaa
      841 gttcaccatt ggcgtggcta gcaatcggcg caacattagg tgaagtaagc ttcaaaaaag
      901 ctgcatctga aaaaacttca tggtattaca gtctagtcaa agtggtattt gtacctgcat
      961 tgaatatcgt gattctagca gttttgacgg caacgaatat tctaccagtc aattttgaag
     1021 cactggcaac aatcgttatc atgatggcta caccagctgc aacagtggca gcagcctatg
     1081 ctatcagttt tgatagggag gcacttttag cctccaatgc atccttactg tcaactattt
     1141 ttgctgtggt actgacacct gtatggatcg ttgtattaca aatgattcat ggcataggtt
     1201 tattttcata agaaatggag gaagaaagta tgagaatcgc atgttacggt gttcgtccga
     1261 acgagataga tttttttgaa aaattgaata tttaccatta tgatctcagt ctttatgaag
     1321 aactgttgac acatgacaat atcgtgtcag caaaggacca tgatgctgta ttgttgaggg
     1381 gaaattgtat agcagatgag accaatcttg caaaaatgca ggaatatggg attcgttatg
     1441 tttttacacg gactgtggga gtcaatcata tcgatctaca agcggcagct gattttggta
     1501 tgacagtagc ccgtgtacca tcgtattcgc caaatgcgat agcagagtta tctttgactt
     1561 ttgccatgat gcttttgaga aatactgcgt atacgacgat ccgaacatct ttcaaagatt
     1621 ttcgagtgga tgagcaaatg tttagtcgtg aaatcagaaa ttgcacagta gggatcatag
     1681 gaacaggcag gatagggttg acggaagctg ttttgtttaa gggactagga gccaaagtga
     1741 tcggctacga tattcatcag acagaagctg ctaaaaaagt attgacgttc cactcgcttg
     1801 aagagttatt agccaaaagc gatattgtca gtattcatgt acctcatatc ccaggtgaaa
     1861 acgatcaaat gatcaatgaa ggatttttag ctcaaatgaa aaaaggtagc atcttgatta
     1921 atacagcccg tggagagctt caagataatg aagctatttt aagagctttg aaaagcaatc
     1981 atttagctgg ttttgcaacc gatgtttttg caaatgaaac agaggttttt ttccgatcct
     2041 tcaagccatg ggagacgatt ccagaccctg ctatccagca attagtagaa atgtacccta
     2101 gagttcttgt aaccccgcat gtaggttcaa atacagatga ggcattatca aatatgattt
     2161 cgacaagttt cgagaacttt cgtgaaataa tagaaactgg aaaaacgaaa aatgaggtat
     2221 ctcttcctaa agcccgtcaa ttaagataag aaaacatgga tattcgtcaa taaaatgttt
     2281 aaaaatagtc caagtaaaca gttgttactt ctgtatactt ggactatttt gtatgggata
     2341 attcgatgac tcaacagtaa tctgtgagct ttttccgccc attctttgtc aattcataac
     2401 aggttagcgg ccggccgatt tccaaatatt tgattctttc gttcaatata tcttgttcta
     2461 ctaaaaaatt caaatatttc ttgacggata tacgtgacag tgagcttttt tgagccagtt
     2521 cttttgttga aaaaatagta tcttctttta gaataaatgc acttattttt tttaaagtca
     2581 gttttgacag acctttaggc aaatcgatga atgatggtat ttcgtgaatg ggagtggtgt
     2641 ggttgaatac ttgatcgatc aatttctgat cggctttttc agtcgtttgg attgtctgag
     2701 agaaagcaag atacttttcg atcgccagct gaaaacgttg caaagtgaac ggtttgatca
     2761 aataatctaa ggcaccgtag cggatcacat tctttatggt ttcgatatca tctgcagcag
     2821 tgatcaaaat cactgggata tcatttcctt gctgtctaag ccaagataaa aaatggatac
     2881 cactttcgcc aggcatgtat acatcaagca gcagcagatc tgcgtttttt tgtctgaaat
     2941 atttttttcc ttcttctaca tttctgaaac tttcgattac gttcatatta gggatgcttt
     3001 ccacaaaagt acgattcaat tctgcaacca ttggatcatc ttcgattatc aggacatttg
     3061 tcatcatttt gatcttcctt ttttggtaat tctagataga cgattgtacc gccatttgtt
     3121 tgtttggaaa attctaaaaa tccaccgtgg cgtttgacga ttgaatggat gagattcaat
     3181 ccaaacccac gattgattcc cttcgtcgaa aaacctagcg taaatatttt ttctgagatt
     3241 tcttctgata tcccgacacc gtcatcgcag acggtcacga tgaatacttc agcttctgtg
     3301 tcatagttta aatgaatccg gacctgtccg tttctttcat gtttttgtag cacggcttct
     3361 tttgcattat caagtaagtt ccctaacact tgcaaaagtg aatggatgga gtcatccatc
     3421 tccattttag ggataaaaga atcgctttct aacagcattt caacagattg ttctttggct
     3481 tcgtttattt tgcctaataa aaaaccggca attgcaggta ctttgatcag acttgagact
     3541 tggccaatct cttttttata attttgattt agctgctgga taaaatgggt gacttcttca
     3601 tacttcttca aatcaatcaa accagaaatg acatgaagtt tattcatgaa ttcatgggtt
     3661 tgagctctca tggagtttat atattgtttc gtccccccaa gctcctccat taatttcatt
     3721 acttctgatc gatttcgtaa agttgcgact gctcctaaaa aacggtcatt gctaaaaaga
     3781 ggtgtaacgc tagaaatagt ttctatccca tttaaataca tggattgagt ccgaacggtc
     3841 gtttgatgct caatcgtctc tttaaacaaa acgtcatata gattatcact gatttttttc
     3901 ctacttcgaa atccaatagc tgctctgttt ggtagaaaat gttttttgca ggtgtattga
     3961 tcaagaggat agttttatca gaagagatgg ctaagattcc ttcatctact tcattttcga
     4021 taatcgtttt ttcacttaac tgttctgcta tttcttgtgg ttcaagtcca agcagtactt
     4081 ttttcatttt ttgggcaaga aaaacggcgc ctatgatgcc tacgctaagt ccaataatca
     4141 atgaaaaaac aatcattttt tgtccatctt tgacttgatt gtcaatcgtg ttgtttgtta
     4201 agccaacaca aactgcacca ataatctctt gatcataata aatagggacg aaatagcgga
     4261 agccgaaccc taacattcct tctttttcag agaaaaattc ttgtcctcca atcacttttt
     4321 tcacatcttc atgacttgag aaaggaagac cgatgtaact tgggtctgcg tgagaatatc
     4381 gaatgccttc agggtccatg acaacgataa aatctaaatt cgatgcaatc atgatttcat
     4441 ttgttattcc ttgaatcgca tgtgtcgggt caccagtctg cagctttttt tggatgagtt
     4501 tatcattggc aacgatttta gcaatgctag agattttatc tgacacacta gatcggttca
     4561 tcgaaagaat aaaattcctg atcaatatgg cagaaacaat caaagaaacg aatgtggaga
     4621 aacaaacgac aaggatgatc gtgttctgga gctttaatgg ttttttctta aaaaaaggaa
     4681 acataggcta cctcgaaaga atttattgtg aaaattttaa catgaaaaca aaaatttaac
     4741 aaattgtcaa cgataaacac aaccttccta taaaaaacac cactgagtcg gtaaaagaag
     4801 ctatacagaa ttttcaaaca ttggtatgat agacgtagaa atggaaggag gcgtgtatat
     4861 gataggggga atcgcacttg agacgattta ttggtataca ttgctgattt gtgcaggatt
     4921 ggcagtccta ttgattattt ttggagatgt attcgatttt gatggcccgc ttgatccgat
     4981 gcttattatc ccttggttaa ctttcacaag cttatttggt tttctaggtg agcgtttaac
     5041 agatcaatca tctttatttt tatttattgc tagtggaggc atatcgatag tgttggtatt
     5101 tttgcttaat ttctatatat tgatgccgat gaaacatgcc gaatcaacga tgtcgtcttc
     5161 tgaaaaaact ctggaaggac aagtagctgt tgtgatcaca ccgatccctg tacgaggtat
     5221 gggagaaatt cagttgaaaa gtgttaccgg ttccattagt cgtccagctt gtttttacag
     5281 cccccaagaa cagacagctg agcggggaac gaaagtattg atcattgaga tcaaggaaag
     5341 aatctgttat gtcatgcctt atgaaagcat gataaaagct taaaggagcg tgtgttaaat
     5401 ggaaatacca gttgttttta tcgcgatcgc aatcgcagct ttgattttac tgatgttgct
     5461 gatcgtcttt gtagcgaagt accagacagc aaaaccggat gaagcgttga tcatcagtgg
     5521 aagttattta ggaagcaaaa atgtgcacat tgatgaaagc aataacaaaa tcaaaattgt
     5581 gcgtggaggt ggcgcattcg tcttgccagt ttttcaacgt tcgaatcgga tcagtctatt
     5641 atcgagtaaa ttggacgtct ccacccctga agtgtatacg gaacaaggcg taccggtgat
     5701 gtgtgatggt acttcgatca tcaaaatcgg ttcatctgta gaagagattg ctacagcagc
     5761 agaacagttt ttaggcaaga caagagaaga actggaaaat gaagcaagag aagtattaga
     5821 aggacacttg cgctcgattc ttggttctat gactgtagaa gaaatctatc aaaatcgcga
     5881 taagttcagt caaagcgtac aagaagtcgc ttctgtagac cttgcaaaaa tggggttgat
     5941 catcgtttct ttcactatca aagaggtcag agacaaaaac ggttatcttg actctttagg
     6001 gaaaccaaga atcgcccaag tcaaaagaga tgcagatata gcagaagcgg aagctctaaa
     6061 agaaacgcgt atcaaaaaag ctcaagcaga aaaagaatca caagcagctg aattacagcg
     6121 acaaacagag atcgcagaat cattaaaaga aaaagaactg aaattagcaa cctacaaaca
     6181 agaacaagat gttgctaaag caaaagcaga ccaagcctac aatttagaaa gtgcccgagc
     6241 acaacaacaa gtaatcgaac aggaaatgca gattaaaatc gtagaacgtc aaaaacaaat
     6301 cgaactggaa gaaaaagaaa tcacacgtcg tgaaaagcaa tatgattcgg aagtgaagaa
     6361 aaaagcagat gccgatcggt acgctaaaga acaagaagca caagctcaaa aagtcaaaga
     6421 agtgactgaa gccgaagcag aacgattccg tgtggaagca ctggcagaag cggaagccaa
     6481 taagacacgt ttagctggac aagcagaagc cgaagcagca ctagcaaaag gaaaagcaga
     6541 agccgaagca aaacaaaaga tcgccaatgc cttcaaggaa tatggcgaag cagctgtctt
     6601 gagtatggtt atcgatatgc taccgcaatt gatgcgcgaa gcagcacagc cgctaggaaa
     6661 tatcgaaaaa atctctgttg tcgatacagg aagcagttca ggagaaacca gtggagcgaa
     6721 ccgagtgaca aattatgcca caaatttgct ttcttctact caagaaacac taaaagcgac
     6781 aacaggactt gacttgaagg aaatcgtaga gaatttgtcg acacgtggga ctactagcca
     6841 agtaaatatg tatgaagcgc caaaagaggc agaacaaaac tcgatgaagg aagaataaag
     6901 tagtacactt caccgttcaa tcaaaaatgg aggaagactt tttaaagttc ttctctccat
     6961 ttttttattt tattgcaaaa gaaaattagt cctcgctatt tttcttttgt ttttggatct
     7021 tagcttctat ctcttgtaaa tttgttctta gcaatcgttc tttcttttgg agttcgacca
     7081 aatcttctga tagttcttcc ttcttttctg tttcaggagg acgaagaaag aaattgatac
     7141 cgattgcaat gaatgtccct ataaaagtat ctagtacacg ttggatagca tacaaagcgg
     7201 attcgccttg agggatgctc aacgcaatca gcaacaacgt agctattgcg gaaatgatac
     7261 cggaattatt gttaatacca tctgacacga cgatcacaat aataactaga ataggaagca
     7321 agatcaattc taccaaaaaa tcattgtgaa aatagctttt gacaaaaaaa taaatgatag
     7381 ctaatgtacc acctaatgta ttgccgatga tcctagactt tccaaaagaa acggttgtag
     7441 tcaagtcctg acgcaaagaa aaaacagcag ataatgcagc aatcatcggg attcctcgtt
     7501 gagtcaaatg aaaaaacaag atacataaaa aaacagctaa ggcacttttg atcgtacgca
     7561 tacctagtcg aaaacggcca aattgcatag agattctcct ttttggtctt tttactatca
     7621 tacctaaact tttgtctgat agctagtttt tcattattca agtataatta aaataaaaaa
     7681 gggggaagta aatgcgtata ggaatgcgaa cagtcaaaac agtgatcagt gtcttctttt
     7741 cgatggtcac agcgggtagc ttatcgctgt tgtattggcc agctgcaggg atcattgcat
     7801 tattaagtgt aggaaatacc aagcgctcta cattgcgtac gggcatgaat cgaatcgcag
     7861 cctttatatt agctacggca acagcactgt tcagtttttt tgctgttggg tatactattt
     7921 ttggcttcag tcttttttta ttcctgttca tcccgcttgc ctctcgcttc aaattaaccg
     7981 aagggatagt agtaaattcg gttttggtca ctcattattt agtggaggaa aacatgtctt
     8041 ggcagctgat cggcaatgaa gcgggattaa tggcgattgg attagtattt gctttgcttg
     8101 caaatgttta catgccagat cgtacgaagc agctgaagga aaaccaaata cagatcgaaa
     8161 aagaatttcg aaatattttg agacaaatgg cagaatttct tttatcagaa aataaagaag
     8221 atgtgcagat caaatgcgaa catttactga cattcattcg cgaaagccaa gaagatgcca
     8281 gagaacatca agaaaactac tggctcagac agccgctata ctacgaaact tacttctcta
     8341 tgagaagagc acaggccaat gtgattaaag acatgctgga aaatctagag agaatccagc
     8401 aaccagctta ttatggaaaa catatctatg gtttattgat ttatactgca gaaacatttt
     8461 cagagtcaaa tgatggcaga caaattttaa cacgtattga agaagtttat gtattgtatc
     8521 ggcaaatgcc tctgccgact agtcgccctg aattcgagga tcgagcagaa ttgttccagt
     8581 ttttgcagtc ttttaaaagt tttattgaaa tcaaggcaga attctcccaa caaaaaatcc
     8641 aaaaatagag gttgtgaatt cagctaatat tcaggtcaaa aagctgattt cacaacctct
     8701 tctttaaacg gtgttctcct gtcaactcct cggtcacaag tcacaatatt caacaaacat
     8761 gagaagctgt tttttgaaat tgctccttgt gactcggagc tgaacggtag gctcacaacc
     8821 tctttcttaa acggtgttca aaagctttcc tctgcggtaa taagtccagc caaacgaaaa
     8881 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     8941 aaccttttaa aattacatat ttttccatag aacttgcatt tgatccaaga tttcgatgac
     9001 atcttttgtc aagaagagag agcgattggg caagtcacct tcaatcatgt gtaccatatt
     9061 ctttatttcg taattcattg cttcagatgt attcccgctt tcgatgaatt cctttgtacc
     9121 atctgtaaat aagatctccg cacgatcagc acgcgggtaa tcagcaatcg tcacataacc
     9181 gttctcaaag gcaacgatac cgacttttgg catttttgcc tgaaaagtca agctgacagc
     9241 agcaagttca ttttctttgt tacgcaagat agtgacggat tgttcgtcca cgcctgtttc
     9301 aaaaggaacc attgtagagg caactacttc aggttgagag ctcaaaaaga aacgagcgaa
     9361 agatacagca tacgtaccga tatccaacag agctcctcca gctaattctg gattgaagaa
     9421 ccggttttta gggtctggtt ctttgtaact gccgaaaggt gcttggatca tttttaaagc
     9481 ccccagtttt cctgtatcca ttattgagcg taactgctga taaagtggca tgttgaagat
     9541 tgtcatcgct tctgctaaga tcaaattctt ttcttctgct aagcgcatag cgtcagccag
     9601 ttctttttta ttcatcgtga tggctttttc acatagaacg tgtttgcctg cttccaatgc
     9661 tgccaagata tggtcgatat gctgtctatt tggtacagca atgtacacga tgtcgatttc
     9721 agcatcgtct aacatttctt gatagcttcc gtaagctttt ggaatattgt aacggtgagc
     9781 gaagttttcc gctttttctg aagttctgga agcaactgct gttagttcgc tcgtctcttg
     9841 attgaagctt tcggcaaact catgtgcgat ggtaccaaga ccaacgattc cccattgata
     9901 ttttttcatc tgaaaacgct ccttcatagt tggattgagt ttcttgtctg tctattatta
     9961 taacaaaaat cacgaagaat aaagaggaga ggacttgaaa cattaaaaaa aatgaaagca
    10021 gcgatttaag aggtgctttt tgatcaaaac ttaagtagaa tagttaaaag gaggggataa
    10081 acgtgcatca agtatatatt gatcttatta tgagtgtatt attagaacag tttgaaactg
    10141 aaaaaaattt tttagatgaa tacttgctga tcagtccaga acaatggcaa aaatggaaag
    10201 atggcgagaa aaatctgcca tctgaagcga tgcagaaagt aaaaaatctt ttcactgatt
    10261 atgaatggat gctaacgcaa aagattttgc gtcaaacgat tatttttccc gaaaaacgaa
    10321 acgttgccgt tgcagagtac aagcaaatca aaacacgcat cgcgcaaaaa tggcttagca
    10381 gcaaagcagc aaaagtcgag ctgatccctt tgaaaaataa tcagcaggct ggtagatacc
    10441 ttgacttgaa agtaagtatt caatatgatg aatggggcta tgatgatatt ttgaattttc
    10501 gtctgccagc agatgttcaa aaacagatcg aaggagaaaa aatcgaactt ctcgattggg
    10561 tgaatgaaaa tctggaagaa acttacgttc atgaggcgaa tggggaacgt gaagaacaac
    10621 gaaaggattt ttcaagtgaa taaattttta ggatttggag cagttgtgat tatgatcgca
    10681 gcaatgggct atgttatttt tagcgatgat gattcgcatg ttcaagccgg caatcaagca
    10741 gtcgaacgaa caagcaccaa aacaactgct agtaaagaaa agaacagtca aacaaccagc
    10801 agcaaggtaa aggacaaaga aacaaaagat cgtacgtcca aacaattgcc agatgttaaa
    10861 acagacgatt ggaagctagt tttggttggt ccagatcaca aattagaaaa agaaattgat
    10921 gagtctactc aattggcatc attaagtaac ggatacttga tcgacaaacg cattgctcca
    10981 gaatatgaag catttgccaa ggctgctgaa gcagctggat ttccattagt gatggtctca
    11041 gcttaccggt ctgtatcatc ccaacaacaa gttttcgaac aaaatgtcca agatgtgatg
    11101 agtcgtcaag gtgtatcaga agaggaggcg acaaagatta ctaaagaaac gattacggag
    11161 ccaggataca gcgaacacca cacaggtctg gcagtcgatg tcgtcgatca aaactggtac
    11221 aacagttatc cttctactgt attggatgca agttacggag atcagcctgg agctaaatgg
    11281 atcgcagata atgctgcgca gtatggattt atcgttcgtt atccaaaagg acacgaagat
    11341 ataactaaga tcacatatga accttggcac ttacgttatg tcggtaaaga aaacgctgag
    11401 tatatcacca aacaccaatt gacgctagaa gaatatctga atgaattaaa tgaaaagtag
    11461 gaattgatca tggcgaaaaa aaagtggaaa aagcaaaagc gcaagaatat ccgttggcca
    11521 gcagtggttg ccggtgtagc agtgatactc ttagcctttg ttttttcctt gaagaatctg
    11581 tcttctcctt ttactgataa tcagcaagaa aatgaagcgc aatcccatca gcaatttatt
    11641 gaacggcttg ttccgcatgc ccaagaacta caagatggtt atggagtgct tcctagtatc
    11701 attttaggac aagctatatt agaatcaaat tggggaaaaa gtaccttggc tagcaaatac
    11761 aataatcttt tcgggatcaa ggcttatggg gatcagaaga aagtaagcct ggaaacaaag
    11821 gaatttgtta acgaagaatg gataactatc caaggagact tcaaagtgta tgattcatgg
    11881 gaacagtcca tggatgacca tacacaactg ttcgtccaag gagtagattg gaatcctgcg
    11941 ctgtatgaaa aagtgattac ggccaccaac tatcaggaag ccgcacaagc attgcaagat
    12001 gcaggttatg cgacagaccc cggttatgcg caaaaaatca tccaggtcat cgagacgtat
    12061 caattgaatc agtatgaccg gagatagcaa aggagaagtg gctgatggca gaacaattta
    12121 cgccagaaat catgacggaa ttgagaaaag atatcgtaag agtaccggaa atcattttag
    12181 aagcaagcgg gatcaagata tttggaaaat tgatccgttc gatcatcttt actacggata
    12241 ttgcgatcat acgtaataca aatgcgaatg cagtaattgc tgtgtatcct tttacacctc
    12301 atccagcgat tactaaaagc attattgagg cagctgacat tccagttttc tccggtgtag
    12361 gtggtggttt gacacaaggt gctcgttctt cctacatgag catgttcgca gaagcgcaag
    12421 gttcgttagg ggtcgttttg aacggtccta cgcctttaga gacagtaaaa gaagtttgtc
    12481 gagtagttga tattccagtt atcagtacgg taacgtcgaa atatacagaa atagatgaaa
    12541 agttagcagc tggtatttcg attatcaata tcagtgcagg taaagaaact gcagaagcag
    12601 tacgccattt tcgtaaaaaa tatcctgaac ttcctatcat tgcgacaggt ggtccaacgg
    12661 aagaaagtat tgctgagacc attcaagcag gagcaaatgc catcacatat acgccaccaa
    12721 gtagcggaga gctttttagt cgtaaaatgg aaaaatatcg ggaaactgag aaaaagaatc
    12781 atgaataaca ggtgagaatc aaaagacgag gaagagcaga tagtggctct tcctcgtctt
    12841 ttgtactcca gtactatttg ttcagaattc aaaaaaaaga gaaatttttt tgttttttta
    12901 cctgtaatgt acataagaag agttaaaacc ttttgttatt attagcattc taacaaaaaa
    12961 atgtcttgat ttattgaagc gctttctata gaatgaaaaa cgaataaaaa ctaaaaaagg
    13021 cacccgacaa tttagtagca gctaaattgt cggggcttga aaagtcagcg ggaactgacc
    13081 aatcaagttg ccatagtcaa tgcttgtgca tcgacgtgta tattgtacct aatttcatac
    13141 agaattacta gttatttttc tgttttttag ggaagagcgc acacgaagaa gcatgcttat
    13201 ttagctatgt caaggatatt gggcagttgt ctgatatcct tttttctttg cgtaattatc
    13261 ctcccatcat tgaagcaaga agtgttttta gcgatgaaag aagaatcagc actatctaat
    13321 ttttcttgaa agtattgagt tgttcatcct aggcagaacg ataaggcaag tcttggtttt
    13381 tattcccctt gaaaaagatc gtttaataaa tatgttctgt ataaaaatac agtatattga
    13441 aaaataacaa tggaagtaga ggtgcagaga atgaagaaaa gaatgagaat catcttagct
    13501 ttaagtgtta tgttattctt cttgacggct tgtgggcaag aagatgacaa aaaaatcacg
    13561 ctgaacatgg cttggtgggg ttcgcaagtc cgacacgatg caacagtaaa agtaatcgag
    13621 ctttatgaag aaaaaaatcc ccatgtgacg atcgattatg aattttatga ttatgaagga
    13681 tattacacaa aattaggtac actagcagca tcagatgata tctgggacat tttccaactg
    13741 aacaatcaat ttcctcaata catcaatcag attgaaccat tagatgaata tgtggagcaa
    13801 gggataattg atatttctaa tgcagatgaa tcctatttag caacgacgac ttacgacggg
    13861 caattagttg gactgtctaa cggggtgaat tcgtttgcga ttgcttacaa taaaaatata
    13921 tttgaacaat taaatatccc tgaaccagct tcaaattgga catgggatga atttgaacaa
    13981 atcagctatc aaatcactga agaattagga atctatgcta tttcaagatt cgaagatttt
    14041 atagctggct gtattattca aataccacaa gttgagaaag gattgaattt ttataatcgg
    14101 gaagatctat ctaacagctt gggatttgaa aatccagact atctaacaga ttttttccag
    14161 ctgcgtaaaa atctggtaga caaaggggcc catccagaac caggtggtgg agctgcaagt
    14221 acgacggatc tgtcttttca ggcggttagg gatagagaag cggcaatggt gtttctatca
    14281 agtaaccagt tgacagaaat attgtcaggg gctcctgaag atatggagat tcagttgatt
    14341 aatccacctc gcagaaaagc agatggagaa tctggtatcc ctttacgttc ttcgcaaatg
    14401 ctgagtatgg caaaaagctc agcccataaa gaagaagctg ctaagtttat tgatttcttt
    14461 caaaacagtg aagaagccaa tgaaatttta aaaggagaac gtggtgtacc aatcatgtcg
    14521 aatattcgag aacagctcgc tcgtgcagat gatgcaactt tgcaagcgac atttgattat
    14581 ttagaaatga tttccgatat ggacaacggc gaagtgaatg tctttgatag tgctttgaat
    14641 ccggaaattc aagaccaata caatctttta gttgaaaaag tcatttatga agaagtaaca
    14701 gcagaagaag cagcagagag tctttatgag tttgcgtcaa acattctagg atagcagcag
    14761 gaggaaggca tgaaaaaaga aaaactaaaa cggttcttgg atcaagacaa tgtggtcggt
    14821 tacgtatttg cttctccatt catcctagga tttctattga taacagtgat accgatgatc
    14881 ttgtcacttt attattcgtt caccgattat cgtttgggaa atccagtctc atggatcggc
    14941 ttggataatt atataaagat gctgagtgat gcccgttttt tgaactccat ccgagtcact
    15001 tttctctatg tatttgcatc tgtacctgtg aaactgatct ttggtctatt gattgcttat
    15061 cttttaacga ggaatatcaa aggaacgact ttctttcgtt ctttgtatta tgtgcctagt
    15121 ttgatcggtg gcagtatttc tatcgctcta gtttggcggg agttgttttc taaaaacggg
    15181 ttagtgaata ttttcctgga acgattaggc gcacctgcgc tttcctggtt tagcgatcaa
    15241 aaactggtca tgatccctct tgttttgatg tctgcttggc aattcggttc ggcaatgatc
    15301 atctttgctg caggtatcaa agagatccca ggctcgtatt atgaagcggc agagatcgat
    15361 ggagcgaaca aatggcagac gttttacaag atcacgttac ctcttctttc gccgatcatt
    15421 ttgtataact tggttatgca gacaatcgcg gcgtttatga cttttacgca ggcatttatc
    15481 attacccaag gtggtccgaa tgatgccacg aacttttatg ctctttatgt gtataaccaa
    15541 gctttttctt ttaattctat ggggtatgct agtgctattt cgtggatcat gctgttgatt
    15601 atgggagctg tcacctttct gattttcaaa tcatcaaaaa aatgggtcgt tgtgtgaatg
    15661 agaggaggaa gaaatgaata atacagtaac gaaatggttt caatacggtt ttattttgct
    15721 attaggtcta gtcatgattt atccggtctt atggatgatc gcaggttctt ttaaaacgga
    15781 tcaagagatt atgagtggtt cgctgaattt gattccagag acttttcaat gggaaaatta
    15841 cgcaaaagga tgggcggggt ttgctggaat ttcatttttt actttcttta ggaattcgtt
    15901 tgtcatctct gcagtttcga cgatagggac agttctgagc tcgacttgta ttgcttacgc
    15961 attatcgaga atcgatttca aaggaaaaag attctggttc gtcgtcatgt tagtaacgat
    16021 gatgattcct gcacaagtga ttttgattcc gcagtttatt atttacaatc ggctgaattt
    16081 ggttgggacg tatgtgccgc tgattttacc acattttttt ggacaagcat ttttcattta
    16141 ccagatcatg caattcatgg tcagcatccc caaagagtta gatgaatcag cgattattga
    16201 cggatgcagc aagtatagtg tctttaccaa aattattttt cctttgctga aaccttcgat
    16261 catcacgacg atcatcatcc agttttattg gaaatgggac gacttcatgg gaccattgat
    16321 ttatttaaac aagccgagat cttacacggt atcgattgcc atcaaattat ttgcagacgc
    16381 tggatctagt acgacttacg cgagtatgtt tgcgatgtct acgttgtcgc tgctaccggt
    16441 ttttctgatt ttcttattct tcaataaata tcttgtccag gggatcagta caagcggtct
    16501 gaagggctga atatgaaaaa gccaatagaa gagatgtttc tttacaagct caactactat
    16561 gtctgtcgga tttttttaac cagtcttatc agtaatgctc ttttcgcttt agttctagga
    16621 tgtttgttca ctttaggatt agtgctgtct gctcgtgttg tctatctgat ttattttttg
    16681 atgatgtatc cttcttatta tctatctgtc cattacttgg cagaaaaaaa ggtatgttgg
    16741 aggaaaggaa aaatcaaacg gatgtgtttc atcattacgt tgtttcagct gtttgtcatt
    16801 atttttgcag ttgattggta tttattagtg ggaaagattg gagcttggta tctgattcct
    16861 tttctattgt gtctgttgct ttttgtatcc catagttttt ttagtacgct aattgatatc
    16921 tgcaaagaag ataaagcaga tagtcttgtt tatttgatca aaaggaatat ctggctgttg
    16981 tcaaaaaacg tcaaggggtt tctgtggtat ttatgccttt tgtatggact gttctggtgt
    17041 atacagcatg attttttact cactgcatta ctgtatccag ggctgccatt tgtcacagta
    17101 ttttgcttaa aagaaaagga gaatgacttt aaatgaagaa aaaatatgta caagtcggac
    17161 ttggtgggag agcgcgtttt ttttatgagg caattgcctc tacgtatcat caaacttgtg
    17221 aactagtcgg tttttgtgat gtatctcaaa caaggatgga ttatgcaaat cggatacttg
    17281 tgaacaagta tggacagaaa aaagtaaaga catgtatgcc atcacaattt gagcaactac
    17341 tggatgacgt acggccggct gaagtcatta ttacgtcagt agatcgaacg catcatgatt
    17401 atatcattcg tgcaatgaaa aaaggatatg atgttatttg cgaaaaacct ctgactatcg
    17461 atgaggaaaa agctcaagaa ataataacgg tccaaaaaca gacgggaaga agcctgcgag
    17521 tcacttttaa ttatcgttat gcaccgtatc acacgaagat aaaggaactg atagaaagtg
    17581 atgtgattgg tgatgtgttt tctatccact tcgaatggct gttaaatacg caacacgggg
    17641 cagattattt cagaagatgg catcgaaata agcacaatag cggcgggctt cttgttcata
    17701 aatctaccca tcattttgat ctagtgaatt tttggttgaa cagccgtccc caaaaggtat
    17761 ttgcattcgg tggattaaac ttttatggga tgcataatgc ggagaaacgc ggagtagaaa
    17821 tattttacag tcgatgctat ggaagcgaac tagctaaagc tgatccattt gctatagacc
    17881 tgtcagccaa tgaggaattg cgagaaatgt atttagaagc agaaaaagac agtggatata
    17941 ttcgtgaccg cagtgtattt tccgatgata tctctattga agatacacta ggcgtaacgg
    18001 tcaaatatga aactggtgcg attttgacgt attcgctaaa cgcgtatatg ccgtgggaag
    18061 gattcaatgt ttctttcaat ggcagcaaag gacggatcga aatgaaagtg atcgaaaagt
    18121 cttacatcaa tgcaggtgga gataaaaagg atgaaggagc agccgttctt cagtccatcc
    18181 atgtctatcc gatgttcggt gagccatatg aggtaatcgt cgaacaagaa gcgggcggac
    18241 atggcggagg agatccgcgt ttattagatg atctatttgg tgaagaaaaa gaagaagatc
    18301 cgtggaatcg ggcagcaact cacgtagatg gtattttgtc gattttgaca ggaatcgcag
    18361 caaatcattc tattgcttca ggaaaagcag tagtgattga cgagcttgtc agcttaaaag
    18421 aataagaata gatttacgga aattacttcc cgtagtttat aaaatcttgc cttattgccg
    18481 ataggctgtc ttttgaacac tatttattcg gaaaaagggg ctatgacatg ctttttgtca
    18541 tgccttcttt ttatgtgggc tgctttttta gataggagaa aataaaaata actatttaaa
    18601 cccgaacaaa aaggaataat gcattaaaaa tcattagatt ttacaaaaaa ataagtaaac
    18661 cacgttgtca gtttcagaaa gtagtgttag actgttttca atatttgaaa caacattttt
    18721 tattcaaata tggcaaactg ccttcacgtg caaaacagtt gcaagaaaat gaatttacac
    18781 tattattagt tttacatacc tacttatata tcgtcataat atggatgaca gtttctagcc
    18841 agtaccgtaa atgctggact ataagtaaaa gattggctat ttcacccttc ttttcttatg
    18901 ataaagtgga gggtgttttt ctttaacaga aacatagtag gtttttgcac aaaagcggtt
    18961 atttcggtat taataaatga taaaggagca aataaagtga aagaattagt tgaacgtatt
    19021 caaaaagacg gacgtatatt aggcgagggc gtattaaaag tcgacagctt cgtcactcac
    19081 caagtagacc ctgtattgat ggaacaaatg ggaaaacggt ttgcagaagt atttaaagaa
    19141 caaggaatca cgaaagttgt gaccattgaa gcttctggga tcgcaccagc tttatttgct
    19201 gcaagagaat tagatgttcc aatgatcttc gctagaaaag caaaaagttt gacgatggat
    19261 gaagaattat tgacagcttc tgtttattct tttacgaaac aagtgacaag tacgatttct
    19321 atttctcgta aatttttatc ttcagaagac aaagtattga ttatcgacga tttcttggca
    19381 aacggacaag cggcaaaagg attgatcgaa ttatgccagc aagctggagc gcaagtagaa
    19441 ggaatcggga tcgtcatcga aaagtctttc caagatggac gtcagttact tgaagaaatg
    19501 ggattgagag tcgtttctct tgcgcgtatt gcttccttga cgaatggaca agttgagttt
    19561 cttgaggagg acgcataatc gtggaaaaag aaacacaaaa tggaaaagct gcggttttag
    19621 ggctgcagca tctgctagcg atgtatgcag gagcagtagc ggtgcctctt ttgatcggta
    19681 caggactagg ctttaatcaa gaacagatga cttatttgat ttcaattgat atctttatgt
    19741 gtggattagc gacattattg caattgacag ttaaccgatt tttcggaatt ggcttgcctg
    19801 tcgttttagg atgtgcgatc caagcagtag ctccactgat cttgatcgga accaatgaag
    19861 gagtaggagc tatctacgga tctattatcg cttctgggat ctttgttgtg ttagtttctg
    19921 gtgtcttttc taaaattaaa aaactattcc ctcctatagt aacaggaacg gttatcaccg
    19981 ttattggttt gactttgatt cctgtagcaa tcgagaaaat gggtggaggt aatgcaacag
    20041 cagctgattt tggtgacaaa acaaatctgc tacttgcatt tgtgacgatt ctcctaattg
    20101 taggcgtgca aatgctggca aaagggttca ttcgttcaat cgcggtattg attggtttag
    20161 tcggaggaag catcttagct gctttcatgg gaatgattga tctgagcgcg attggagatg
    20221 cgccgttgtt ccatgttcca caaccctttt attttggtaa accaactttc gacgtatggt
    20281 cgattttact aatgatcatc atttccatgg tcagcatggt agaatcaaca ggtgtatatt
    20341 ttgcacttgg tgacattact gggaaaaatg ttggagaaga agaattgaaa aagggatatc
    20401 gtgcagaagg acttgccgtt atcttaggcg gtattttcaa tacattccct tatactggct
    20461 tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa aacaagaaaa cccatctatt
    20521 tctctgcatt cttcttgatt gttttaggat tattccctaa aatcggtgcg ttagcacaaa
    20581 tcatccctga gccggtactt ggtggaggaa tgttagtcat gttcgggatg gtggcagtcc
    20641 aaggaatccg tatgctgttg gaagttgatt tcagcaatga taaaaatcta ttgattgttg
    20701 ctgtttctgt tggatttggg ctgggtttca atgttacgcc gacattattc agtcaaatgc
    20761 cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat gagtagcatt acggctatca
    20821 tattgaattt gatctttaat ggattgaaaa aagatgaaga aatattggaa gaagccttca
    20881 agaagtaata gagcaaaaga aaggttgtga aatcagcgtt ttgacctgat tgttagagaa
    20941 acaaacggaa aaatagttcc tcgtattttg ccgtttgttt gagctaatac cgagggtcag
    21001 ctgattgaac accgttgaag gaagaggtcg tgagcctgcc gttcatctcc gagtcacaag
    21061 gaacaatttc aaaaaaaagc ttctcatgtt tgttgaatat tgttccttgt gaccgaggag
    21121 ttggcaggag aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc
    21181 ttttttttat ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat
    21241 ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa
    21301 acaatgacag caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa
    21361 gaagcatgtc aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct
    21421 catcgaaccc cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa
    21481 atcattattg caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca
    21541 actttgccgg ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt
    21601 ctttcgattg tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg
    21661 ggagcaatta atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg
    21721 gaggctaaat tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat
    21781 caacttggtt aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt
    21841 aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc
    21901 tgtgagcgat tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga
    21961 cacctttgcg ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa
    22021 cgtcagtgta gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga
    22081 cttgcttgcg attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat
    22141 cgtgattgct ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg
    22201 gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta
    22261 tgttttgaaa agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg
    22321 tgttttagaa gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg
    22381 tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca
    22441 tgaaacgatt gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc
    22501 ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac
    22561 aaaaacaggc gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta
    22621 ttctattgaa gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg
    22681 gccattagga gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga
    22741 acaactgctg gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta
    22801 tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga
    22861 catccagcaa acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa
    22921 catgttagaa cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata
    22981 ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat
    23041 cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt
    23101 ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac
    23161 attgggagaa gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac
    23221 tgcttatggc tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt
    23281 tacggaaatc attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac
    23341 acacggtgtc catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga
    23401 aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat
    23461 ctcaggagca gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa
    23521 acaattgggg atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc
    23581 tgaatatttc tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat
    23641 acgcggattg caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa
    23701 aggttcttct gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct
    23761 agccagagtg gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga
    23821 aagagatatt tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt
    23881 gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa
    23941 catgaagcgg aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa
    24001 attgatcgat aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc
    24061 gtacgcttgg gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac
    24121 atcggtatta tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa
    24181 cgttgacgtg atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca
    24241 attaaaatag aatagcaaaa ataatcgagt agaggggaaa gccaactgac cttccgcccc
    24301 tctcacacca ccgtacgtac ggacccgtat acggcggttc aataagttga gtgtacgaac
    24361 tggtacttca aggagatgtc ctcatatcct tggcgggcca gttctttgtc tgttaatgat
    24421 cgatgaagaa tatgactttt ggagactgac caaatacctt ttcttgtatt tgaccattca
    24481 tacgctttcg ctttatcgat ccctaaacga atcagatttt ttcttttggt tcttggattc
    24541 ttccattgtt tccaaatata ttgtctgatt cttcgcttca accaaccgtt taactccctc
    24601 atgaattctt tcatctcacc aattccatag tagttgatcc aaccagtcat tagttgcttg
    24661 atttctttta aaataagttg gatacttctt ccacggtttc ttttagtgag ttgtttgagc
    24721 ttgcttttca cacgctgttt tgcttttgcg tgagggcgaa ttttaatacc tttctttgtt
    24781 ggcagaatgc agaatccaag aaactttcgt ttcaatggac ttcctaccgc actcttttct
    24841 cgatttaccg tcaactttag ttgttcttcg agaaattgaa tggatttctt catcacacgc
    24901 tgtcctgctc gtttgcttct aacatagata ttgcagtcat cggcgtatcg aacaaattta
    24961 tgtcctagat tttctagcac ttgatcaaat tcgttcaggt agatgttact taacagggga
    25021 gagagattgc ctccttgggg cgttccttct tcagttgttg tgatgagtcc attttccatt
    25081 acaccactca ttaagtattg acggatcagt tttaggactt gtttgtcctt tactttccgc
    25141 tcaatgaaat acatcagctt atcgtgattt acggtgtcaa agtaggcttt catatcaatg
    25201 tccaccacat atcggtaacc ttgttcataa taagattttg ctttcttcac tgcgtcatgt
    25261 gcgctgcgat ttgggcgaaa cccataacta ttatctgaga attctggttc aaatagcggt
    25321 tgtaacactt gattgattgc ctgttgaagc atacgatcaa taaccgtggg aattcctagt
    25381 tttcttacac cgccgtttgg tttcggtatt tctactcgca aaacaggctg aggtttatag
    25441 ttcccttggc gtaaattgga tagtaattcc tcgcggtgct ctttcaagta aggaagcagt
    25501 tgatcgacgg tcatcccatc tgcaccagcc gcccctttat ttctgactac ttgaagatac
    25561 gcttgattga gattattccg atctagaatt ctcccaaaca gattttctac accatcttgt
    25621 ttcctctgtt caccgcaaga agtgctgcgc acttcatcat acccttcgtg ttccacacta
    25681 gctctttgat gatagccaag ttttcttgtt ttctgcggtc gtcgcatctt ctcacacctc
    25741 ctaaattatt caagattctt attgttcagt ccttcgtaac ggtcagtgtc gttactacta
    25801 tgacctcggc tgacttctca tgattcacca tcatgtcgcc atgatcgttg tgctggtttg
    25861 ttttcattca acgcccacaa caccctcatg agatctcccc cggtaagagt gaaaactttc
    25921 ctcccatgta actgctagat ttactgtata ggtttcgggc agtattggac tttgtcttgt
    25981 gatgaagact tatccgacct aattcagcct tgtatctagt ttctgttcgt cagttcagga
    26041 gtttgcgtcc gacttccttc agccacaacc tcacgattgc cgccttgtct ttcgctaaca
    26101 gttcctactg ccaagcctgt agtggacttt caccaccaag ttttcaccca tgcagggcgc
    26161 acaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    26221 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    26281 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    26341 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    26401 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    26461 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    26521 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    26581 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    26641 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    26701 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    26761 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    26821 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    26881 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    26941 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    27001 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    27061 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    27121 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    27181 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    27241 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    27301 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaag
    27361 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    27421 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    27481 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    27541 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    27601 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    27661 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    27721 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    27781 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    27841 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    27901 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    27961 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    28021 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    28081 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    28141 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    28201 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    28261 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    28321 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    28381 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    28441 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    28501 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    28561 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    28621 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    28681 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    28741 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    28801 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    28861 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    28921 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    28981 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    29041 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    29101 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    29161 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    29221 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    29281 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    29341 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    29401 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    29461 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    29521 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    29581 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    29641 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    29701 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    29761 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    29821 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    29881 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    29941 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    30001 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    30061 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    30121 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    30181 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    30241 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    30301 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    30361 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    30421 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    30481 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    30541 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    30601 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    30661 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    30721 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    30781 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    30841 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    30901 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    30961 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    31021 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    31081 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    31141 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    31201 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    31261 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    31321 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    31381 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    31441 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    31501 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    31561 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    31621 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    31681 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    31741 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    31801 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    31861 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    31921 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    31981 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    32041 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    32101 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    32161 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    32221 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    32281 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    32341 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    32401 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    32461 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    32521 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    32581 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    32641 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    32701 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    32761 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    32821 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    32881 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    32941 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    33001 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    33061 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    33121 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    33181 gtccaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    33241 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    33301 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    33361 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    33421 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    33481 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    33541 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    33601 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    33661 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    33721 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    33781 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    33841 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    33901 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    33961 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    34021 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    34081 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    34141 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    34201 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    34261 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    34321 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    34381 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    34441 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    34501 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    34561 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    34621 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    34681 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    34741 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    34801 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    34861 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    34921 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    34981 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    35041 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    35101 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    35161 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    35221 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    35281 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    35341 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    35401 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    35461 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    35521 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    35581 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    35641 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    35701 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    35761 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    35821 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    35881 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    35941 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    36001 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    36061 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    36121 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    36181 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    36241 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    36301 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    36361 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    36421 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    36481 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    36541 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    36601 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    36661 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    36721 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    36781 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    36841 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    36901 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    36961 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    37021 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    37081 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    37141 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    37201 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    37261 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    37321 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    37381 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    37441 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    37501 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    37561 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    37621 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    37681 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    37741 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    37801 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    37861 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    37921 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    37981 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    38041 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    38101 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    38161 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    38221 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    38281 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    38341 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    38401 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    38461 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    38521 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    38581 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    38641 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    38701 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    38761 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    38821 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    38881 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    38941 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    39001 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    39061 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    39121 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    39181 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    39241 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    39301 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    39361 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    39421 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    39481 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    39541 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    39601 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    39661 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgtgg taatacgaat
    39721 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    39781 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    39841 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    39901 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    39961 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    40021 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    40081 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    40141 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    40201 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    40261 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    40321 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    40381 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    40441 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    40501 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    40561 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    40621 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    40681 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    40741 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    40801 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    40861 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    40921 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    40981 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    41041 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    41101 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    41161 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    41221 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    41281 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    41341 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    41401 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    41461 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    41521 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    41581 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    41641 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    41701 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    41761 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    41821 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    41881 caagggctaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    41941 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    42001 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    42061 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    42121 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    42181 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    42241 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    42301 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    42361 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    42421 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    42481 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    42541 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    42601 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    42661 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    42721 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    42781 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    42841 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    42901 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    42961 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    43021 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    43081 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    43141 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    43201 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    43261 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    43321 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    43381 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    43441 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    43501 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    43561 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    43621 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    43681 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    43741 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    43801 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    43861 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    43921 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    43981 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    44041 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    44101 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    44161 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    44221 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    44281 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    44341 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    44401 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    44461 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    44521 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    44581 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    44641 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    44701 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    44761 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    44821 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    44881 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    44941 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    45001 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    45061 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    45121 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    45181 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    45241 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    45301 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    45361 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    45421 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    45481 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    45541 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    45601 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    45661 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    45721 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    45781 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    45841 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    45901 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    45961 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    46021 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    46081 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    46141 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    46201 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    46261 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    46321 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    46381 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    46441 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    46501 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    46561 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    46621 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    46681 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    46741 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    46801 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    46861 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    46921 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    46981 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    47041 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    47101 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    47161 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    47221 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    47281 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    47341 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    47401 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    47461 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    47521 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    47581 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    47641 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    47701 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    47761 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    47821 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    47881 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    47941 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    48001 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    48061 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    48121 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    48181 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    48241 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    48301 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    48361 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    48421 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    48481 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    48541 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    48601 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    48661 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    48721 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    48781 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    48841 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    48901 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    48961 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    49021 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    49081 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    49141 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    49201 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    49261 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    49321 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    49381 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    49441 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    49501 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    49561 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    49621 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    49681 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    49741 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    49801 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    49861 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    49921 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    49981 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    50041 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    50101 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    50161 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    50221 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    50281 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    50341 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    50401 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    50461 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    50521 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    50581 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    50641 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    50701 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    50761 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    50821 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    50881 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    50941 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    51001 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    51061 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    51121 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    51181 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    51241 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    51301 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    51361 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    51421 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    51481 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    51541 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    51601 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    51661 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    51721 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    51781 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    51841 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    51901 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    51961 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    52021 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    52081 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    52141 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    52201 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    52261 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    52321 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    52381 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    52441 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    52501 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    52561 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    52621 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    52681 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    52741 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    52801 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    52861 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    52921 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    52981 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    53041 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    53101 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    53161 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    53221 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    53281 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    53341 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    53401 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    53461 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    53521 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    53581 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    53641 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    53701 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
    53761 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    53821 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    53881 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    53941 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    54001 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    54061 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    54121 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    54181 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    54241 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    54301 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    54361 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    54421 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    54481 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    54541 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    54601 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    54661 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    54721 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    54781 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    54841 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    54901 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    54961 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    55021 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    55081 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    55141 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    55201 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    55261 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    55321 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    55381 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    55441 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    55501 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    55561 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    55621 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    55681 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    55741 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    55801 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    55861 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    55921 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    55981 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    56041 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    56101 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    56161 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    56221 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    56281 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    56341 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    56401 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    56461 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    56521 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    56581 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    56641 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    56701 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    56761 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    56821 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    56881 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    56941 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    57001 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    57061 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    57121 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    57181 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    57241 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    57301 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    57361 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    57421 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    57481 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    57541 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    57601 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    57661 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    57721 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    57781 aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc
    57841 tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta
    57901 aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca
    57961 cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc
    58021 gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa
    58081 aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga
    58141 agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt
    58201 gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata
    58261 cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa
    58321 atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat
    58381 tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt
    58441 atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat
    58501 caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct
    58561 gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa
    58621 cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa
    58681 tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta
    58741 gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt
    58801 ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg
    58861 attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc
    58921 cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga
    58981 gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag
    59041 ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta
    59101 aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat
    59161 tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata
    59221 gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac
    59281 tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct
    59341 ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg
    59401 atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat
    59461 cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat
    59521 cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa
    59581 gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta
    59641 ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg
    59701 cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct
    59761 cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat
    59821 gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat
    59881 aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga
    59941 tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca
    60001 gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct
    60061 tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc
    60121 tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa
    60181 tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag
    60241 gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat
    60301 catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc
    60361 agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa
    60421 aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac
    60481 gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct
    60541 gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta
    60601 cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt
    60661 gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt
    60721 tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa
    60781 ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg
    60841 gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg
    60901 atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa
    60961 cggctactat gccatgttca acggaggact atttggcagg ggattaggta acagtatcca
    61021 gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga
    61081 actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat
    61141 tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg
    61201 cgcaatgttt ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt
    61261 gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat
    61321 ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata
    61381 ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa
    61441 tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg
    61501 cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac
    61561 aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag
    61621 aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg
    61681 cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata
    61741 tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc
    61801 caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg
    61861 gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc
    61921 acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct
    61981 ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac
    62041 agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc
    62101 agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac
    62161 gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag
    62221 gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg
    62281 tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac
    62341 tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa
    62401 ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact
    62461 ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc
    62521 tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag
    62581 taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct
    62641 tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca
    62701 cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat
    62761 tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa
    62821 ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac
    62881 gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg
    62941 cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg
    63001 atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt
    63061 tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt
    63121 gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt
    63181 tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa
    63241 atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag
    63301 cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg
    63361 aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata
    63421 ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg
    63481 caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc
    63541 ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa
    63601 tccatctgca tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa
    63661 aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc
    63721 ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga
    63781 ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt
    63841 ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac
    63901 agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa
    63961 tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat
    64021 catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag
    64081 atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag
    64141 gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag
    64201 gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag
    64261 taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct
    64321 atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg
    64381 ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg
    64441 tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata
    64501 tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca
    64561 aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag
    64621 aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata
    64681 acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg
    64741 aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt
    64801 ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa
    64861 aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc
    64921 agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca
    64981 aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa
    65041 accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt
    65101 tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat
    65161 ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc
    65221 gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt
    65281 cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta
    65341 tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac
    65401 aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata
    65461 tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc
    65521 aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga
    65581 cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca
    65641 tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa
    65701 atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa
    65761 gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga
    65821 cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga
    65881 cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca
    65941 agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg
    66001 aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc
    66061 ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct
    66121 tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc
    66181 gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg
    66241 cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac
    66301 ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga
    66361 aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata
    66421 acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa
    66481 aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat
    66541 gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta
    66601 gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca
    66661 gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa
    66721 catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga
    66781 tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag
    66841 tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg
    66901 ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc
    66961 atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga
    67021 agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa
    67081 aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact
    67141 tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt
    67201 tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact
    67261 gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc
    67321 agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac
    67381 tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct
    67441 tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta
    67501 attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca
    67561 ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa
    67621 ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt
    67681 atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa
    67741 ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta
    67801 aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa
    67861 gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat
    67921 tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga
    67981 atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa
    68041 aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc
    68101 tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg
    68161 ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc
    68221 agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt
    68281 atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac
    68341 agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa
    68401 aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac
    68461 agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct
    68521 tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg
    68581 tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa
    68641 cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg
    68701 ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt
    68761 ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac
    68821 caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc
    68881 agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat
    68941 t
//
LOCUS       DACCVU010000002        63549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCVU010000002 DACCVU010000000
VERSION     DACCVU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.002"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(162..854)
                     /locus_tag="IVW29_000062"
     CDS             complement(162..854)
                     /locus_tag="IVW29_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7837576.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(857..1642)
                     /locus_tag="IVW29_000063"
     CDS             complement(857..1642)
                     /locus_tag="IVW29_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7837577.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            1879..3279
                     /gene="lacG"
                     /locus_tag="IVW29_000064"
     CDS             1879..3279
                     /gene="lacG"
                     /locus_tag="IVW29_000064"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7837578.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3304..3621
                     /locus_tag="IVW29_000065"
     CDS             3304..3621
                     /locus_tag="IVW29_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7837579.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            3634..5307
                     /locus_tag="IVW29_000066"
     CDS             3634..5307
                     /locus_tag="IVW29_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7837580.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5321..6952
                     /locus_tag="IVW29_000067"
     CDS             5321..6952
                     /locus_tag="IVW29_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7837581.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7443..9008)
                     /gene="guaA"
                     /locus_tag="IVW29_000068"
     CDS             complement(7443..9008)
                     /gene="guaA"
                     /locus_tag="IVW29_000068"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7837582.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9067..9990)
                     /locus_tag="IVW29_000069"
     CDS             complement(9067..9990)
                     /locus_tag="IVW29_000069"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7837583.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10186..10791
                     /locus_tag="IVW29_000070"
     CDS             10186..10791
                     /locus_tag="IVW29_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7837584.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11099..12115
                     /locus_tag="IVW29_000071"
     CDS             11099..12115
                     /locus_tag="IVW29_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7837585.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12256..13557
                     /locus_tag="IVW29_000072"
     CDS             12256..13557
                     /locus_tag="IVW29_000072"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7837586.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            13603..14265
                     /gene="deoC"
                     /locus_tag="IVW29_000073"
     CDS             13603..14265
                     /gene="deoC"
                     /locus_tag="IVW29_000073"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7837587.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14286..14684
                     /locus_tag="IVW29_000074"
     CDS             14286..14684
                     /locus_tag="IVW29_000074"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7837588.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            14750..15832
                     /locus_tag="IVW29_000075"
     CDS             14750..15832
                     /locus_tag="IVW29_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7837589.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16044..17606
                     /locus_tag="IVW29_000076"
     CDS             16044..17606
                     /locus_tag="IVW29_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7837590.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            17599..18732
                     /locus_tag="IVW29_000077"
     CDS             17599..18732
                     /locus_tag="IVW29_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837591.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            18732..19688
                     /locus_tag="IVW29_000078"
     CDS             18732..19688
                     /locus_tag="IVW29_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837592.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            19787..20953
                     /gene="deoB"
                     /locus_tag="IVW29_000079"
     CDS             19787..20953
                     /gene="deoB"
                     /locus_tag="IVW29_000079"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7837593.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            20977..21795
                     /locus_tag="IVW29_000080"
     CDS             20977..21795
                     /locus_tag="IVW29_000080"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7837594.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            21812..22519
                     /gene="deoD"
                     /locus_tag="IVW29_000081"
     CDS             21812..22519
                     /gene="deoD"
                     /locus_tag="IVW29_000081"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7837595.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(22817..23503)
                     /gene="gpmA"
                     /locus_tag="IVW29_000082"
     CDS             complement(22817..23503)
                     /gene="gpmA"
                     /locus_tag="IVW29_000082"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7837596.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(23675..24256)
                     /locus_tag="IVW29_000083"
     CDS             complement(23675..24256)
                     /locus_tag="IVW29_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7837597.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24389..25072)
                     /gene="rpiA"
                     /locus_tag="IVW29_000084"
     CDS             complement(24389..25072)
                     /gene="rpiA"
                     /locus_tag="IVW29_000084"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7837598.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25391..25804
                     /gene="rpsL"
                     /locus_tag="IVW29_000085"
     CDS             25391..25804
                     /gene="rpsL"
                     /locus_tag="IVW29_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7837599.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            25888..26358
                     /gene="rpsG"
                     /locus_tag="IVW29_000086"
     CDS             25888..26358
                     /gene="rpsG"
                     /locus_tag="IVW29_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7837600.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            26452..28539
                     /gene="fusA"
                     /locus_tag="IVW29_000087"
     CDS             26452..28539
                     /gene="fusA"
                     /locus_tag="IVW29_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7837601.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            28711..29898
                     /gene="tuf"
                     /locus_tag="IVW29_000088"
     CDS             28711..29898
                     /gene="tuf"
                     /locus_tag="IVW29_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7837602.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30305..30613
                     /gene="rpsJ"
                     /locus_tag="IVW29_000089"
     CDS             30305..30613
                     /gene="rpsJ"
                     /locus_tag="IVW29_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7837603.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            30644..31273
                     /gene="rplC"
                     /locus_tag="IVW29_000090"
     CDS             30644..31273
                     /gene="rplC"
                     /locus_tag="IVW29_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7837604.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31301..31924
                     /gene="rplD"
                     /locus_tag="IVW29_000091"
     CDS             31301..31924
                     /gene="rplD"
                     /locus_tag="IVW29_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7837605.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            31924..32214
                     /gene="rplW"
                     /locus_tag="IVW29_000092"
     CDS             31924..32214
                     /gene="rplW"
                     /locus_tag="IVW29_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7837606.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32253..33086
                     /gene="rplB"
                     /locus_tag="IVW29_000093"
     CDS             32253..33086
                     /gene="rplB"
                     /locus_tag="IVW29_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7837607.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33129..33407
                     /gene="rpsS"
                     /locus_tag="IVW29_000094"
     CDS             33129..33407
                     /gene="rpsS"
                     /locus_tag="IVW29_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7837608.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            33430..33777
                     /gene="rplV"
                     /locus_tag="IVW29_000095"
     CDS             33430..33777
                     /gene="rplV"
                     /locus_tag="IVW29_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7837609.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            33791..34447
                     /gene="rpsC"
                     /locus_tag="IVW29_000096"
     CDS             33791..34447
                     /gene="rpsC"
                     /locus_tag="IVW29_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7837610.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            34450..34884
                     /gene="rplP"
                     /locus_tag="IVW29_000097"
     CDS             34450..34884
                     /gene="rplP"
                     /locus_tag="IVW29_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7837611.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            34874..35062
                     /gene="rpmC"
                     /locus_tag="IVW29_000098"
     CDS             34874..35062
                     /gene="rpmC"
                     /locus_tag="IVW29_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7837612.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35087..35353
                     /gene="rpsQ"
                     /locus_tag="IVW29_000099"
     CDS             35087..35353
                     /gene="rpsQ"
                     /locus_tag="IVW29_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7837613.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            35411..35779
                     /gene="rplN"
                     /locus_tag="IVW29_000100"
     CDS             35411..35779
                     /gene="rplN"
                     /locus_tag="IVW29_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7837614.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            35816..36124
                     /gene="rplX"
                     /locus_tag="IVW29_000101"
     CDS             35816..36124
                     /gene="rplX"
                     /locus_tag="IVW29_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7837615.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36152..36691
                     /gene="rplE"
                     /locus_tag="IVW29_000102"
     CDS             36152..36691
                     /gene="rplE"
                     /locus_tag="IVW29_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7837616.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            36710..36895
                     /locus_tag="IVW29_000103"
     CDS             36710..36895
                     /locus_tag="IVW29_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7837617.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            36931..37329
                     /gene="rpsH"
                     /locus_tag="IVW29_000104"
     CDS             36931..37329
                     /gene="rpsH"
                     /locus_tag="IVW29_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7837618.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37361..37897
                     /gene="rplF"
                     /locus_tag="IVW29_000105"
     CDS             37361..37897
                     /gene="rplF"
                     /locus_tag="IVW29_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7837619.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38058..38414
                     /gene="rplR"
                     /locus_tag="IVW29_000106"
     CDS             38058..38414
                     /gene="rplR"
                     /locus_tag="IVW29_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7837620.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            38435..38935
                     /gene="rpsE"
                     /locus_tag="IVW29_000107"
     CDS             38435..38935
                     /gene="rpsE"
                     /locus_tag="IVW29_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7837621.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            38950..39129
                     /gene="rpmD"
                     /locus_tag="IVW29_000108"
     CDS             38950..39129
                     /gene="rpmD"
                     /locus_tag="IVW29_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7837622.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39173..39613
                     /gene="rplO"
                     /locus_tag="IVW29_000109"
     CDS             39173..39613
                     /gene="rplO"
                     /locus_tag="IVW29_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7837623.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            39613..40908
                     /gene="secY"
                     /locus_tag="IVW29_000110"
     CDS             39613..40908
                     /gene="secY"
                     /locus_tag="IVW29_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7837624.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            40970..41617
                     /locus_tag="IVW29_000111"
     CDS             40970..41617
                     /locus_tag="IVW29_000111"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7837625.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            41808..42026
                     /gene="infA"
                     /locus_tag="IVW29_000112"
     CDS             41808..42026
                     /gene="infA"
                     /locus_tag="IVW29_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7837626.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42059..42175
                     /gene="rpmJ"
                     /locus_tag="IVW29_000113"
     CDS             42059..42175
                     /gene="rpmJ"
                     /locus_tag="IVW29_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7837627.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42193..42558
                     /gene="rpsM"
                     /locus_tag="IVW29_000114"
     CDS             42193..42558
                     /gene="rpsM"
                     /locus_tag="IVW29_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7837628.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            42586..42975
                     /gene="rpsK"
                     /locus_tag="IVW29_000115"
     CDS             42586..42975
                     /gene="rpsK"
                     /locus_tag="IVW29_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7837629.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43055..43993
                     /locus_tag="IVW29_000116"
     CDS             43055..43993
                     /locus_tag="IVW29_000116"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7837630.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44021..44401
                     /gene="rplQ"
                     /locus_tag="IVW29_000117"
     CDS             44021..44401
                     /gene="rplQ"
                     /locus_tag="IVW29_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7837631.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            44673..45512
                     /locus_tag="IVW29_000118"
     CDS             44673..45512
                     /locus_tag="IVW29_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7837632.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            45488..46357
                     /locus_tag="IVW29_000119"
     CDS             45488..46357
                     /locus_tag="IVW29_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7837633.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46347..47144
                     /locus_tag="IVW29_000120"
     CDS             46347..47144
                     /locus_tag="IVW29_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7837634.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47347..48093
                     /gene="truA"
                     /locus_tag="IVW29_000121"
     CDS             47347..48093
                     /gene="truA"
                     /locus_tag="IVW29_000121"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7837635.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(48548..51217)
                     /locus_tag="IVW29_000122"
     CDS             complement(48548..51217)
                     /locus_tag="IVW29_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7837636.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(51455..51643)
                     /locus_tag="IVW29_000123"
     CDS             complement(51455..51643)
                     /locus_tag="IVW29_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7837637.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            51913..52275
                     /locus_tag="IVW29_000124"
     CDS             51913..52275
                     /locus_tag="IVW29_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7837638.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            52327..54009
                     /locus_tag="IVW29_000125"
     CDS             52327..54009
                     /locus_tag="IVW29_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7837639.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54126..56162
                     /gene="recG"
                     /locus_tag="IVW29_000126"
     CDS             54126..56162
                     /gene="recG"
                     /locus_tag="IVW29_000126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7837640.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56211..57212
                     /gene="plsX"
                     /locus_tag="IVW29_000127"
     CDS             56211..57212
                     /gene="plsX"
                     /locus_tag="IVW29_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7837641.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            57334..57576
                     /gene="acpP"
                     /locus_tag="IVW29_000128"
     CDS             57334..57576
                     /gene="acpP"
                     /locus_tag="IVW29_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7837642.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            57844..58848
                     /locus_tag="IVW29_000129"
     CDS             57844..58848
                     /locus_tag="IVW29_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7837643.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            58849..59793
                     /locus_tag="IVW29_000130"
     CDS             58849..59793
                     /locus_tag="IVW29_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7837644.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            59793..60755
                     /locus_tag="IVW29_000131"
     CDS             59793..60755
                     /locus_tag="IVW29_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837645.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            60782..61696
                     /locus_tag="IVW29_000132"
     CDS             60782..61696
                     /locus_tag="IVW29_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837646.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            61722..63503
                     /locus_tag="IVW29_000133"
     CDS             61722..63503
                     /locus_tag="IVW29_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7837647.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT        20274 a        11640 c        13901 g        17734 t
ORIGIN      
        1 gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac
       61 taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt
      121 gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg
      181 gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc
      241 tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg
      301 atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca
      361 gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca
      421 gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa
      481 atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt taatggaatc
      541 tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt
      601 tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc
      661 tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca
      721 tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata
      781 tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat
      841 ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca
      901 gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga
      961 gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat
     1021 tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt
     1081 cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt
     1141 ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat
     1201 ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat
     1261 caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca
     1321 atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg
     1381 ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag
     1441 aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct
     1501 tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt
     1561 gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa
     1621 gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa
     1681 acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg
     1741 ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg
     1801 acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag
     1861 atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca
     1921 gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat
     1981 gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa
     2041 tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt
     2101 gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt
     2161 tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat
     2221 cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata
     2281 gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg
     2341 agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca
     2401 ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct
     2461 catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt
     2521 cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt
     2581 ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca
     2641 actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt
     2701 cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc
     2761 aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac
     2821 ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat
     2881 aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac
     2941 ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac
     3001 gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac
     3061 tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag
     3121 gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg
     3181 tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac
     3241 tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa
     3301 aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac
     3361 gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt
     3421 gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa
     3481 attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg
     3541 caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg
     3601 tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa
     3661 aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac
     3721 ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg
     3781 gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc
     3841 tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg
     3901 gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg
     3961 ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca
     4021 acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt
     4081 aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct
     4141 ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc
     4201 gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt
     4261 acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg
     4321 ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca
     4381 attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa
     4441 caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact
     4501 ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag
     4561 gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt
     4621 gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta
     4681 aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt
     4741 ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta
     4801 gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc
     4861 aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc
     4921 gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc
     4981 agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtgcg
     5041 atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg
     5101 gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca
     5161 ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag
     5221 ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt
     5281 cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt
     5341 gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt
     5401 tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga
     5461 aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa
     5521 aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg
     5581 agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc
     5641 aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc
     5701 tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc
     5761 attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga
     5821 tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg
     5881 gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc
     5941 tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga
     6001 tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt
     6061 aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat
     6121 gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca
     6181 tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg
     6241 gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat
     6301 tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa
     6361 cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat
     6421 gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt
     6481 gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag
     6541 cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa
     6601 gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca
     6661 ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta
     6721 cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa
     6781 aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat
     6841 tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc
     6901 ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga
     6961 tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa
     7021 tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc
     7081 aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg
     7141 atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt
     7201 aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa
     7261 aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca
     7321 aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa
     7381 gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct
     7441 ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg
     7501 acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata
     7561 cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag
     7621 tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa
     7681 tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca
     7741 gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga
     7801 ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct
     7861 aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata
     7921 tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca
     7981 gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca
     8041 gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt
     8101 ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcattgact
     8161 ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct
     8221 ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt
     8281 aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg
     8341 tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag
     8401 ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg
     8461 gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata
     8521 gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt
     8581 accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca
     8641 gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct
     8701 acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga
     8761 atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt
     8821 ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg
     8881 cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg
     8941 ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg
     9001 tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt
     9061 aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag
     9121 aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac
     9181 atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg
     9241 ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa
     9301 ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc
     9361 gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa
     9421 gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga
     9481 gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag
     9541 catctccata tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa
     9601 tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa
     9661 ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact
     9721 tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt
     9781 actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac
     9841 atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag
     9901 ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc
     9961 cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga
    10021 actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt
    10081 gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata
    10141 aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac
    10201 tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc
    10261 agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt
    10321 tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta
    10381 gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt
    10441 gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt
    10501 aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa
    10561 acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa
    10621 attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag
    10681 aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa
    10741 atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa
    10801 aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct
    10861 ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag
    10921 gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct
    10981 gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt
    11041 cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat
    11101 gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca
    11161 gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat
    11221 cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc
    11281 aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga
    11341 aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca
    11401 tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat
    11461 cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca
    11521 aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat
    11581 cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac
    11641 gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg
    11701 atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg
    11761 agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat
    11821 cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga
    11881 attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc
    11941 agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta
    12001 tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt
    12061 gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg
    12121 cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta
    12181 aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac
    12241 aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag
    12301 ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat
    12361 tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc
    12421 acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa
    12481 ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta
    12541 gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt
    12601 tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg
    12661 gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga
    12721 aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat
    12781 tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca
    12841 atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga
    12901 cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt
    12961 atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag
    13021 gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta
    13081 ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg
    13141 cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat
    13201 caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca
    13261 atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga
    13321 attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct
    13381 gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg
    13441 acgatctata gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt
    13501 gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg
    13561 aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg
    13621 atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa
    13681 gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg
    13741 aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga
    13801 gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat
    13861 gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa
    13921 aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt
    13981 gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca
    14041 ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat
    14101 gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc
    14161 cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc
    14221 gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga
    14281 agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag
    14341 cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga
    14401 tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca
    14461 cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg
    14521 ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct
    14581 gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg
    14641 ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt
    14701 cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc
    14761 aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg
    14821 caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc
    14881 attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg
    14941 actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt
    15001 ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga atgctggatt
    15061 caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa
    15121 caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt
    15181 atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac
    15241 gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt
    15301 tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa
    15361 agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca
    15421 aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg
    15481 tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg
    15541 tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt
    15601 cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc
    15661 agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt
    15721 tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga
    15781 aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt
    15841 ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt
    15901 catttatcca agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt
    15961 tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga
    16021 aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata
    16081 ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag
    16141 gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc
    16201 tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga
    16261 tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc
    16321 ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag
    16381 ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat
    16441 tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag
    16501 aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg
    16561 tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag
    16621 gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct
    16681 gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac
    16741 aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag
    16801 cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat
    16861 tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg
    16921 gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag
    16981 aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca
    17041 ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata
    17101 tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg
    17161 gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg
    17221 ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag
    17281 caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc
    17341 gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca
    17401 aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc
    17461 gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg
    17521 aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag
    17581 aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct
    17641 gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt
    17701 cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata
    17761 ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct
    17821 aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc
    17881 agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc
    17941 gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca
    18001 ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta
    18061 agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc
    18121 aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc
    18181 tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg
    18241 atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct
    18301 gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct
    18361 ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa
    18421 acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca
    18481 gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc
    18541 atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc
    18601 ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa
    18661 aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg
    18721 ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt
    18781 attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg
    18841 taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact
    18901 tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg
    18961 tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc
    19021 atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca
    19081 aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt
    19141 tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac
    19201 ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat
    19261 tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca
    19321 atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg
    19381 gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag
    19441 ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg
    19501 cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta
    19561 tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc
    19621 tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat
    19681 caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc
    19741 tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt
    19801 acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg
    19861 tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca
    19921 tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga
    19981 tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg
    20041 ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt
    20101 ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa
    20161 accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga
    20221 tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat
    20281 tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc
    20341 gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg
    20401 aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt
    20461 gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca
    20521 aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa
    20581 agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt
    20641 atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg
    20701 aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca
    20761 cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt
    20821 ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc
    20881 agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa
    20941 attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta
    21001 caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt
    21061 ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat
    21121 gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat
    21181 gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt
    21241 cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca
    21301 gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg
    21361 ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat
    21421 gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca
    21481 gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat
    21541 tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat
    21601 gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg
    21661 ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa
    21721 gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca
    21781 ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg
    21841 cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga
    21901 gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg
    21961 caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac
    22021 catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac
    22081 atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc
    22141 gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga
    22201 tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt
    22261 aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt
    22321 agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc
    22381 gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga
    22441 agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga
    22501 aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac
    22561 atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta
    22621 agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag
    22681 gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag
    22741 ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc
    22801 ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt
    22861 cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac
    22921 cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt
    22981 tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg
    23041 tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt
    23101 catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac
    23161 gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac
    23221 cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa
    23281 gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag
    23341 tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt
    23401 cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat
    23461 tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta
    23521 agataggttt atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag
    23581 atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac
    23641 gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt
    23701 tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa
    23761 taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg
    23821 catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta
    23881 atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat
    23941 agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc
    24001 gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa
    24061 gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa
    24121 cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa
    24181 agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat
    24241 aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt
    24301 aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt
    24361 ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa
    24421 cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta
    24481 ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta
    24541 gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg
    24601 tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt tgggagccat
    24661 aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat
    24721 caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag
    24781 cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca
    24841 aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt
    24901 cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc
    24961 gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc
    25021 cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt
    25081 ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa
    25141 aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt
    25201 gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa
    25261 gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt
    25321 tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag
    25381 gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga
    25441 aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa
    25501 cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa
    25561 accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac
    25621 tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg
    25681 tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac
    25741 tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc
    25801 ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga
    25861 acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat
    25921 gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg
    25981 gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga cttgatcaaa
    26041 gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct
    26101 gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt
    26161 cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc
    26221 ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca
    26281 ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt
    26341 gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat
    26401 agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga
    26461 gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa
    26521 acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact
    26581 catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc
    26641 acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca
    26701 ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta
    26761 actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca
    26821 acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac
    26881 ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa
    26941 ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct
    27001 gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac
    27061 cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa
    27121 gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt
    27181 atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag
    27241 aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc accgttagat
    27301 atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac
    27361 gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt
    27421 ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca
    27481 acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca
    27541 gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact
    27601 actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca
    27661 gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt
    27721 gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa
    27781 acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt
    27841 atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa
    27901 actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt
    27961 caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt
    28021 gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt
    28081 ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct
    28141 aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct
    28201 gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc
    28261 atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact
    28321 gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt
    28381 gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa
    28441 ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa
    28501 gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac cggatagtcc
    28561 tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc
    28621 gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa
    28681 tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa
    28741 ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct
    28801 gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc
    28861 gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat
    28921 gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa
    28981 aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac
    29041 ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac
    29101 atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt
    29161 gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta
    29221 gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt
    29281 gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca
    29341 ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt
    29401 cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa
    29461 gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct
    29521 gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt
    29581 ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta
    29641 tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca
    29701 caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa
    29761 atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc
    29821 gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt
    29881 acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta
    29941 atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta ttgcaggatc
    30001 aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag tcagtatttt
    30061 ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt
    30121 ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag acgtaatcag
    30181 aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt tgccacgaac
    30241 gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg aaggagggaa
    30301 caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc gtattttaga
    30361 tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg tgtctggtcc
    30421 aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc ataaatacaa
    30481 agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc
    30541 aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg taaacatcga
    30601 aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca aaggaatctt
    30661 agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa ttccagtaac
    30721 tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa cggatggcta
    30781 cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa
    30841 aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat tcaaaaatgt
    30901 tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc aagcaggaga
    30961 cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta tcaaacgcca
    31021 cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat
    31081 gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg
    31141 caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat
    31201 cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca aatcagctgt
    31261 gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg tagcattatt
    31321 caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct tcggaattga
    31381 acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg
    31441 aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca
    31501 aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc gtggaggtgg
    31561 cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa aagttcgtcg
    31621 tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga
    31681 aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg ctaacttatc
    31741 tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg ctttatcagc
    31801 acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt
    31861 atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg aggtgcttgc
    31921 ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc
    31981 atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa
    32041 caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt
    32101 gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg caaagcgatc
    32161 gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga ataagcacta
    32221 atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac aaacctacct
    32281 caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca tcaacacctg
    32341 aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac ggccgcatca
    32401 ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta
    32461 acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac cgttcagcta
    32521 acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca cctaaaggac
    32581 tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc
    32641 ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg aaacctggta
    32701 aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt aaagaaggca
    32761 aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct gcatgccgtg
    32821 ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt aaagctggac
    32881 gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac cctaatgatc
    32941 acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca gtttctcctt
    33001 ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca gacaaactta
    33061 ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat tttgagagga
    33121 ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa
    33181 aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt
    33241 caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga cgtaaacatg
    33301 taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt gcacctacaa
    33361 gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt gaggggagga
    33421 taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac agttcgcact
    33481 tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt cgcagatgca
    33541 atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa agtattgatg
    33601 tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt ggtagtaagc
    33661 gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca
    33721 gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga aaaataagga
    33781 gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca tcatccgcga
    33841 ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg aagatttaag
    33901 aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga
    33961 acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta tggttattgg
    34021 taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa ctggtaaaag
    34081 aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat tagtaggtga
    34141 aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat
    34201 ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa
    34261 cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc ttcatacttt
    34321 acgtgcggac attgattacg catgggaaga agcagataca acatacggaa aactaggagt
    34381 taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg agaaaggagg
    34441 gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg
    34501 cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt gcaagctgtt
    34561 gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat gactcgttac
    34621 atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta tactgccaaa
    34681 gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt tgcaccagta
    34741 aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg
    34801 ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg
    34861 ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg aaatgcttga
    34921 tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag caacaggtca
    34981 attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt
    35041 gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga ctgaaggaag
    35101 aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata aaacaattac
    35161 cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga actattctaa
    35221 gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg tgagaattat
    35281 ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc
    35341 agttattatc taataacacg agattttcct atatagattg aatgcaaaat ctgaaaggag
    35401 gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact caggcgcacg
    35461 tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta acatcggtga
    35521 tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt
    35581 cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg gttcatatat
    35641 taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc gtggaacacg
    35701 tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact
    35761 agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt
    35821 tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt
    35881 tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa
    35941 aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga tggaagcgcc
    36001 aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc gagttggcta
    36061 caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag ttttagataa
    36121 ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa
    36181 agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc aaacacctaa
    36241 agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt
    36301 agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga ttacaaaagc
    36361 taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg caaaagttac
    36421 attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt cactaccacg
    36481 tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa actatacttt
    36541 aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag ataaagtacg
    36601 tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc gtgagttgtt
    36661 gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg tggctaaaaa
    36721 atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt atactcgttg
    36781 cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc gtatttgctt
    36841 ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt
    36901 aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat tgcagatttt
    36961 ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt acctgcatca
    37021 aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa
    37081 tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc
    37141 gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa
    37201 gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc gacttctgaa
    37261 ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac
    37321 gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta tcggaaataa
    37381 agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg ttacagttaa
    37441 aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga atatcgaagg
    37501 aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac
    37561 aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt
    37621 agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg tattgaacgt
    37681 tggttactca catccagtag aaatcacacc accagctgga gtaactgttg aagtaccagc
    37741 taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa
    37801 cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt atgttggcga
    37861 atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa
    37921 gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata catttttact
    37981 tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag tacaataaaa
    38041 ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt caaaagagac
    38101 atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg aacgtttttc
    38161 gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta acgctagcaa
    38221 gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca gcacaagctg
    38281 tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta gtctttgacc
    38341 gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg
    38401 gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc caaaacattt
    38461 ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg
    38521 tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg taggatttgg
    38581 tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa
    38641 aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa tcggtgcttt
    38701 tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg
    38761 accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta aatctttagg
    38821 ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg
    38881 tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg gataaggagg
    38941 acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg acgtcctcaa
    39001 aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc agtggttaag
    39061 ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt ggacgttgaa
    39121 gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac ctatgaaact
    39181 tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac
    39241 ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg
    39301 cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa
    39361 acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg atgtattgaa
    39421 ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa
    39481 aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga aattaactgt
    39541 gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg gtggatcaat
    39601 cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga cattagatcg
    39661 aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca tattactgta
    39721 ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt caacatgttg
    39781 aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg ggtttcacca
    39841 tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt
    39901 gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac tcgatatctg
    39961 acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa tcaatacgct
    40021 caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg tttgattcta
    40081 acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa aggtatcgga
    40141 aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga aagtatcaga
    40201 gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa atctgctatt
    40261 tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac atttttccaa
    40321 caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc gcctacaagc
    40381 agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt tgctagttcg
    40441 tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg ttctgaaaac
    40501 tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc tacgatttat
    40561 acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa
    40621 ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc tggtaagggg
    40681 actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc agtcttcctt
    40741 ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc acaatcaatc
    40801 ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac tgcgaaacag
    40861 ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga aagcgtgttg
    40921 aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa tgaacctcat
    40981 tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca ttgatgctta
    41041 tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa acgaaacagc
    41101 tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg atgaagtaac
    41161 aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct tcttattaga
    41221 tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa aagatttgaa
    41281 taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct
    41341 agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt tcaaccctac
    41401 aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga
    41461 taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg aaccaatctt
    41521 ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc
    41581 tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt agccgacttg
    41641 cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc agtccgactt
    41701 ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg cagtttccgc
    41761 cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag
    41821 atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg
    41881 aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt atgcattaca
    41941 tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg
    42001 gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag gtattatcat
    42061 gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg
    42121 acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag gataattgga
    42181 ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa acgtgtagta
    42241 gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat cttagctgat
    42301 gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac agatgctatc
    42361 cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac
    42421 atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg tggattgcct
    42481 gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc tcgcgctatc
    42541 gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa
    42601 gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca tatccattct
    42661 acattcaata acactatcat catgatcact gatactcacg gaaatgcatt agcgtggtca
    42721 tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg
    42781 gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt tgaagtaaca
    42841 gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc tacaggttta
    42901 gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg ccctccaaaa
    42961 cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt cattgaacaa
    43021 gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa aaccaagaat
    43081 cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc ttgaaagagg
    43141 gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt tgccaggtgc
    43201 tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt
    43261 tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg
    43321 ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa ctgctggcga
    43381 tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct gtagtgtctc
    43441 tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc
    43501 agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt caatctacac
    43561 acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc gtgaagattt
    43621 tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg aagcaatgag
    43681 tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa ctgatgaagc
    43741 aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat
    43801 gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat
    43861 caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag tccgtaactt
    43921 aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt taggtttacg
    43981 taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc gtaaactagg
    44041 acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt tattaatcaa
    44101 cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat
    44161 gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg
    44221 taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat cagctttaca
    44281 aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat acacacgcat
    44341 tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta
    44401 aaatcacact aataacatca ctttatagat gattctttta gagtgttatg atgttggaag
    44461 atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg gggagtaagc
    44521 actcatcata aagtgttttt ttataccttc aattacctgt catgacagat atgtttgaag
    44581 gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt
    44641 agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa ttagaaaaga
    44701 taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc tcctttacaa
    44761 ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa tcgacacttg
    44821 ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa
    44881 tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt tttcaaaatc
    44941 cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt cttgaaaatc
    45001 aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa
    45061 tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa caacgtgttg
    45121 ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa gcaaccagta
    45181 tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc aaagaagaaa
    45241 gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat gccaatcgga
    45301 tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa attttttctg
    45361 ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg
    45421 cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa aggatggtgg
    45481 attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga atacaccatt
    45541 tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt atactgcatt
    45601 agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg cgcttgttaa
    45661 gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata cggataataa
    45721 gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag aagcacagct
    45781 attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg gtgtcagcga
    45841 agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg atgaaagcta
    45901 tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag caatcgctgg
    45961 tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag gacttgatcc
    46021 ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac agcagatcac
    46081 tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt atgtctatgt
    46141 gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc aaaacattga
    46201 atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag aagaattaat
    46261 ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag cagacgctat
    46321 cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt cgttatatgc
    46381 ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc agtttttatt
    46441 ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg gcatttttca
    46501 cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga ggcattcgcc
    46561 cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca agtggcggag
    46621 aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg aacggcctct
    46681 ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg cttactacac
    46741 cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa gccgtacggt
    46801 tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg cctacattga
    46861 tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat tttggtgaag
    46921 gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta tttgtcagca
    46981 gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac aaaggtgggg
    47041 aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg gttatcattg
    47101 cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc agtgggccgc
    47161 tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg tatgacaaga
    47221 aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct gaaaagaaag
    47281 aacgaaaaaa ttttatagaa aaatcattat tccagataac cttttaggaa caaaggagag
    47341 aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg gaacaaattt taatggattc
    47401 caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg
    47461 gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg agtacatgcg
    47521 atcgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg aatgcgtttc
    47581 gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat tgtttcagaa
    47641 gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt ggatataggg
    47701 aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc acttgatttg
    47761 gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac ctctttttgt
    47821 gcatcaggaa gctcgataga ggataaaata cgtacgatct acgaggctaa gatggaggta
    47881 aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt gtataagatg
    47941 atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc tgaagatagt
    48001 atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc acaccctgaa
    48061 ggactttatt tgtatgaagt gaaatatgaa taagaggtta tgatagaagt ggtcagcttc
    48121 aagaaataag aaggtattta cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat
    48181 tttcgaagaa gctacttctg gaacaccgtt taaggaagag gtcgtgaaat cagcgttttg
    48241 acctgagtat tagagaaaca aacgggaaaa tagctcctca tattttgccg tttgttgagc
    48301 taataccgaa ggtcagctga ttgaacaccg tttaagaaag aggtcgtgag cctgtcattt
    48361 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    48421 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    48481 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    48541 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    48601 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    48661 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    48721 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    48781 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    48841 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    48901 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    48961 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    49021 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    49081 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    49141 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    49201 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    49261 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    49321 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    49381 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    49441 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    49501 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    49561 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    49621 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    49681 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    49741 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    49801 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    49861 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    49921 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    49981 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    50041 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    50101 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    50161 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    50221 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    50281 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    50341 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    50401 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    50461 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    50521 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    50581 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    50641 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    50701 aaatcactaa tggaagataa aaaaatgcat ccagccacat gatgatctcc gaatgtgcag
    50761 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    50821 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    50881 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    50941 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    51001 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    51061 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    51121 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    51181 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    51241 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    51301 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    51361 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    51421 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    51481 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    51541 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    51601 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    51661 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    51721 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    51781 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    51841 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    51901 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    51961 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    52021 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    52081 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    52141 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    52201 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    52261 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    52321 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    52381 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    52441 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    52501 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    52561 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    52621 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    52681 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    52741 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    52801 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    52861 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    52921 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    52981 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    53041 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    53101 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    53161 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    53221 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    53281 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    53341 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    53401 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    53461 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    53521 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    53581 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    53641 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    53701 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    53761 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    53821 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    53881 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    53941 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    54001 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    54061 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    54121 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    54181 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    54241 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    54301 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    54361 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    54421 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    54481 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    54541 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    54601 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    54661 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    54721 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    54781 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    54841 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    54901 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    54961 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    55021 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    55081 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    55141 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    55201 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    55261 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    55321 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    55381 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    55441 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    55501 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    55561 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    55621 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    55681 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    55741 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    55801 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    55861 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    55921 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    55981 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    56041 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    56101 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    56161 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    56221 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    56281 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    56341 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    56401 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    56461 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    56521 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    56581 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    56641 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    56701 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    56761 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    56821 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    56881 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    56941 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    57001 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    57061 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    57121 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    57181 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    57241 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    57301 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    57361 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    57421 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    57481 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    57541 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    57601 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    57661 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    57721 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    57781 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    57841 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    57901 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    57961 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    58021 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    58081 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    58141 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    58201 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    58261 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    58321 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    58381 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    58441 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    58501 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    58561 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    58621 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    58681 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    58741 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    58801 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    58861 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    58921 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    58981 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    59041 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    59101 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    59161 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    59221 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    59281 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    59341 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    59401 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    59461 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    59521 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    59581 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    59641 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    59701 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    59761 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    59821 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    59881 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    59941 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    60001 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    60061 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    60121 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    60181 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    60241 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    60301 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    60361 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    60421 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    60481 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    60541 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    60601 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    60661 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    60721 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    60781 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    60841 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    60901 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    60961 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    61021 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    61081 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    61141 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    61201 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    61261 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    61321 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    61381 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    61441 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    61501 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    61561 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    61621 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    61681 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    61741 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    61801 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    61861 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    61921 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    61981 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    62041 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    62101 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    62161 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    62221 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    62281 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    62341 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    62401 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    62461 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    62521 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    62581 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    62641 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    62701 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    62761 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    62821 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    62881 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    62941 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    63001 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    63061 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    63121 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    63181 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    63241 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    63301 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    63361 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    63421 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    63481 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    63541 cggtttttt
//
LOCUS       DACCVU010000003        57146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCVU010000003 DACCVU010000000
VERSION     DACCVU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.003"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            52..1347
                     /locus_tag="IVW29_000134"
     CDS             52..1347
                     /locus_tag="IVW29_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7837648.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1555..1953)
                     /locus_tag="IVW29_000135"
     CDS             complement(1555..1953)
                     /locus_tag="IVW29_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7837649.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2143..2910)
                     /locus_tag="IVW29_000136"
     CDS             complement(2143..2910)
                     /locus_tag="IVW29_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7837650.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(2945..4168)
                     /locus_tag="IVW29_000137"
     CDS             complement(2945..4168)
                     /locus_tag="IVW29_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7837651.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            5149..5952
                     /locus_tag="IVW29_000138"
     CDS             5149..5952
                     /locus_tag="IVW29_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7837652.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            6537..6992
                     /locus_tag="IVW29_000139"
     CDS             6537..6992
                     /locus_tag="IVW29_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837653.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            7111..8169
                     /locus_tag="IVW29_000140"
     CDS             7111..8169
                     /locus_tag="IVW29_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7837654.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            8211..10160
                     /locus_tag="IVW29_000141"
     CDS             8211..10160
                     /locus_tag="IVW29_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7837655.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            10163..13804
                     /locus_tag="IVW29_000142"
     CDS             10163..13804
                     /locus_tag="IVW29_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837656.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            14204..14542
                     /locus_tag="IVW29_000143"
     CDS             14204..14542
                     /locus_tag="IVW29_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837657.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            14652..14873
                     /locus_tag="IVW29_000144"
     CDS             14652..14873
                     /locus_tag="IVW29_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837658.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            14893..17049
                     /locus_tag="IVW29_000145"
     CDS             14893..17049
                     /locus_tag="IVW29_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837659.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            17061..17294
                     /locus_tag="IVW29_000146"
     CDS             17061..17294
                     /locus_tag="IVW29_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7837660.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            17284..18150
                     /locus_tag="IVW29_000147"
     CDS             17284..18150
                     /locus_tag="IVW29_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837661.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            18165..20135
                     /locus_tag="IVW29_000148"
     CDS             18165..20135
                     /locus_tag="IVW29_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837662.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            20302..20496
                     /locus_tag="IVW29_000149"
     CDS             20302..20496
                     /locus_tag="IVW29_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837663.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFKTIS"
     gene            20530..20865
                     /locus_tag="IVW29_000150"
     CDS             20530..20865
                     /locus_tag="IVW29_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837664.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(21122..22705)
                     /locus_tag="IVW29_000151"
     CDS             complement(21122..22705)
                     /locus_tag="IVW29_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837665.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            22827..23411
                     /locus_tag="IVW29_000152"
     CDS             22827..23411
                     /locus_tag="IVW29_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837666.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            23422..23667
                     /locus_tag="IVW29_000153"
     CDS             23422..23667
                     /locus_tag="IVW29_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837667.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            23673..24395
                     /locus_tag="IVW29_000154"
     CDS             23673..24395
                     /locus_tag="IVW29_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837668.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            24526..25269
                     /locus_tag="IVW29_000155"
     CDS             24526..25269
                     /locus_tag="IVW29_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837669.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            25275..25640
                     /locus_tag="IVW29_000156"
     CDS             25275..25640
                     /locus_tag="IVW29_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837670.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            25656..26186
                     /locus_tag="IVW29_000157"
     CDS             25656..26186
                     /locus_tag="IVW29_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837671.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            26348..26656
                     /locus_tag="IVW29_000158"
     CDS             26348..26656
                     /locus_tag="IVW29_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837672.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            26652..27071
                     /locus_tag="IVW29_000159"
                     /pseudo
     CDS             26652..27071
                     /locus_tag="IVW29_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27208..28008
                     /locus_tag="IVW29_000160"
     CDS             27208..28008
                     /locus_tag="IVW29_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837673.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            28045..28227
                     /locus_tag="IVW29_000161"
     CDS             28045..28227
                     /locus_tag="IVW29_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837674.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            28338..28655
                     /locus_tag="IVW29_000162"
     CDS             28338..28655
                     /locus_tag="IVW29_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837675.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            28658..28890
                     /locus_tag="IVW29_000163"
                     /pseudo
     CDS             28658..28890
                     /locus_tag="IVW29_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29005..29484)
                     /locus_tag="IVW29_000164"
     CDS             complement(29005..29484)
                     /locus_tag="IVW29_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837676.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            29604..30941
                     /locus_tag="IVW29_000165"
     CDS             29604..30941
                     /locus_tag="IVW29_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7837677.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            30964..31491
                     /locus_tag="IVW29_000166"
     CDS             30964..31491
                     /locus_tag="IVW29_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837678.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            31478..32041
                     /locus_tag="IVW29_000167"
     CDS             31478..32041
                     /locus_tag="IVW29_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837679.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            32463..33200
                     /locus_tag="IVW29_000168"
     CDS             32463..33200
                     /locus_tag="IVW29_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837680.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(33217..33633)
                     /locus_tag="IVW29_000169"
     CDS             complement(33217..33633)
                     /locus_tag="IVW29_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837681.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            34025..35272
                     /locus_tag="IVW29_000170"
     CDS             34025..35272
                     /locus_tag="IVW29_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837682.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            35540..37930
                     /locus_tag="IVW29_000171"
     CDS             35540..37930
                     /locus_tag="IVW29_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7837683.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            37979..39814
                     /locus_tag="IVW29_000172"
     CDS             37979..39814
                     /locus_tag="IVW29_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7837684.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            39889..40746
                     /locus_tag="IVW29_000173"
     CDS             39889..40746
                     /locus_tag="IVW29_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7837685.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            40736..41845
                     /locus_tag="IVW29_000174"
     CDS             40736..41845
                     /locus_tag="IVW29_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7837686.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            42184..42984
                     /locus_tag="IVW29_000175"
     CDS             42184..42984
                     /locus_tag="IVW29_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837687.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            43072..43878
                     /locus_tag="IVW29_000176"
     CDS             43072..43878
                     /locus_tag="IVW29_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837688.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(43895..45322)
                     /locus_tag="IVW29_000177"
     CDS             complement(43895..45322)
                     /locus_tag="IVW29_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP7837689.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            45643..46512
                     /locus_tag="IVW29_000178"
     CDS             45643..46512
                     /locus_tag="IVW29_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7837690.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            46532..46879
                     /locus_tag="IVW29_000179"
     CDS             46532..46879
                     /locus_tag="IVW29_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837691.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            46892..48154
                     /locus_tag="IVW29_000180"
     CDS             46892..48154
                     /locus_tag="IVW29_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837692.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            48234..49610
                     /locus_tag="IVW29_000181"
     CDS             48234..49610
                     /locus_tag="IVW29_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7837693.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            49607..50236
                     /locus_tag="IVW29_000182"
     CDS             49607..50236
                     /locus_tag="IVW29_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837694.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            50255..50734
                     /locus_tag="IVW29_000183"
     CDS             50255..50734
                     /locus_tag="IVW29_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837695.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            51236..51289
                     /locus_tag="IVW29_000184"
                     /pseudo
     CDS             51236..51289
                     /locus_tag="IVW29_000184"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51293..51892
                     /locus_tag="IVW29_000185"
     CDS             51293..51892
                     /locus_tag="IVW29_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7837696.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            51908..52384
                     /locus_tag="IVW29_000186"
     CDS             51908..52384
                     /locus_tag="IVW29_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837697.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            52401..53303
                     /locus_tag="IVW29_000187"
     CDS             52401..53303
                     /locus_tag="IVW29_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837698.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            53376..53567
                     /locus_tag="IVW29_000188"
     CDS             53376..53567
                     /locus_tag="IVW29_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837699.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            53731..54318
                     /locus_tag="IVW29_000189"
     CDS             53731..54318
                     /locus_tag="IVW29_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837700.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            54318..55217
                     /locus_tag="IVW29_000190"
     CDS             54318..55217
                     /locus_tag="IVW29_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7837701.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            55217..56131
                     /locus_tag="IVW29_000191"
     CDS             55217..56131
                     /locus_tag="IVW29_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837702.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            56183..56398
                     /locus_tag="IVW29_000192"
     CDS             56183..56398
                     /locus_tag="IVW29_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837703.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            56845..>57146
                     /locus_tag="IVW29_000193"
     CDS             56845..>57146
                     /locus_tag="IVW29_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7837704.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT        20995 a         8138 c        11066 g        16947 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt
     1321 catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc
     1381 tgtccaaatt attctaagat ttctctttac caacatcatt tgacaaagag cccaatttaa
     1441 aagctctcag aacgcttctg agagctttta atatattctt tgataatttg tctttcttaa
     1501 atccatacgt cctaaaacaa gctgtttttg cttaatatga tcatctgaga gacatcaaat
     1561 caacttttct ctcaacaaat cattcaaagc tttcgacaga ttaatattct cttcagccgc
     1621 taaaacattt aaatagtgtt ccacagtcac tgattttcga tcataatttt gcttgtttcg
     1681 agctttagca actttcgtat tagcttttac gacaaataaa ttaacattct cagatcgctt
     1741 gattcttgca tttaactctt gaacaggcgt aggcgatgga atttcttttt cttgcaactc
     1801 taaaagcacc aagtatcctt ccaatacttc ttgcgccatc tcaaaagcat cttctaaagt
     1861 ttcaccatgc gtatataagt ctggaaaatc tgggaattta actgcatatt catttccttt
     1921 ccattcgaat actgcacaaa aaaagtattt catcgcattg cacctctcta attaaaaaat
     1981 aatttttgct ttttggtttc aaaaaaagaa tcgcctacca aaaaattgat aggcgatcac
     2041 aagctaatta acctctaaag gagattaata ccttgctccc aaggcactac atatcttaac
     2101 atacgtctga agcatcttca aacgagtaag atatattttg cattaaccta attttttttc
     2161 tctataaggt ttttcatcaa actcactaga aatattaaac tggacagcaa aattgtaatt
     2221 tctgcctttt ctaactttag caaaatcaat cttcagcatt gatttttcat taatttcttc
     2281 aactgattcc cttaaaacac gtctatcaaa attcgatatt gttttatact cattttctgt
     2341 tttcgtaatc tcacgaattt taactatttc aatcagggga ttcttatata tttcagcttt
     2401 ctcaaaatct cctttatttt tggcgataag atatttttta taatacatcg ccaaatattt
     2461 gtataacacg atgctatatt ttttttgaaa agatgctaag tctttaaaaa caaagaacgg
     2521 atacttcttt ttgattgaga caattaaggg aattacatct tctgaaaaac tgaaagatac
     2581 actatgctct tctaaatccc aatgaatccg atcaatagca gatacagtag tatacgtctt
     2641 atatttcttg tctagattcg atatttcata aactgcttgt ctttgcaaag tgttaactat
     2701 ggtttttagt tccgtttcaa gattttgatc aacatccatg taattaataa tcgctttaac
     2761 aggtaaatct actacgcctg ttttctttag ctttgtgggt gagatataac tgaacagcaa
     2821 ttgataaagt tcatacgact ttaaatccat tttcatatca attgttgcta agaactcctc
     2881 attgtacttt gtcagttcat cttttttcaa aagtttttcc ttccttcacg cattttttca
     2941 gttgttaatc ataaaggttc acataaggca aataactcgt aattgatttt tcgagatatt
     3001 taacagtatt ttttattgtg taatcctcct tatgagtagc aacgtaagat aggtgattga
     3061 caactgattc aaatcctcta tctttggata aacgatcata aagcggataa actttattct
     3121 gaagtcggct cataagttta agatcgcata agtcggcaga actgatcaaa aagtttttga
     3181 ttagtaattt agtgtatttg ctattcatag ctttcaagta cgattgattc attttctctg
     3241 ttttatcttc tgcgcctttt aaataagcag ggtctctttg ttcttgctta taaggtaccg
     3301 gaactgacat ggtacgtgta agcaagaact ttatgtttgt ataagtatga cccttcttga
     3361 taagctcata gtcaatttta aaatcagtgt atttattcag ttgggcaatc ggttctttaa
     3421 ttacacgtct ttcaaagtct cgaaaagctt tatatttttt ctcaatacta aatttctttc
     3481 gtaaatctgc aatggagaaa acaggatttt ttaatctatc aagtgatttt gtactccatg
     3541 ttaagttatc tctatttgct tcatacagat taaaatcttt agtgaataat tgaaaaaaac
     3601 tgatcgcata cttgcttgtc aagttaacta cttgatcaaa tgtatattgc gtaaaatgat
     3661 cttctaagtt cgttaataaa ggcataattg aactactaaa tttaatagat actgcatcag
     3721 aaaaatcatt gaattcagta atttctagcg gtgagatgac tttgtattca gtcttaccgt
     3781 taggtttagt tctttgaatt ttaatgacag attgctgatg caatttttca ataacttgtt
     3841 ttaatcttga atgttttgaa gaagatgaac tagaagacat caacgcaaat aaagcctttt
     3901 tagatacttt gacggtatta ttaaaaggcg gatttttagg gtcaagatgt cctatagcgt
     3961 aaagataaat tttttgtggc atagcatcca tttcaacaat gctatctaca agttgattcg
     4021 ataaagtaac agttaaatca aactttttta gattgctttc ggtagtttca aatttcgggt
     4081 ttacattctt ttctgttatt ttcatacgat tgcctcttct ttctaagctt ggtacgagct
     4141 taaaaacttg gaactggtac gattccattg aataagtata gacctgtttt cgatacgtta
     4201 caataagaaa tgtatcgtaa atttataaca taactcaatg tcagtaaaac acattttatt
     4261 ttttccaatc aaatttcatt gaaaatacag cagaaatcgc tattttagaa cattttcaaa
     4321 ataaaaatcc ttttcaataa aatacgaaat tataaaaatt accaaaatta aaaaacaaat
     4381 atttttctag tttttagaaa taaattcaca tactttttct ttttttggat atattcggaa
     4441 taccacatcg ctttttgtcc acgtttgcat cgctttttgt ccacattcac atcgcttttt
     4501 gtccatgttc gcatcgcttt atgtccaccc taaaatctat ttaagattga tataaagcct
     4561 ttttggaggt tgctaaagtt ttaaagatta aaatatatat tataaatata aataaaaggg
     4621 gaagctgaag cttcctcctt ttcctttttt tcaattattc aaagttacac aagcaaaata
     4681 aactcctaga tagtgttagt tttttttaag attttttatt tttaagattt tgaaataaga
     4741 gaggagtaat cctcctttat taaatatctg ttaatatatc tgttaaatat ctgttaatat
     4801 atctgttagg ggaggcttaa tctcttggga gagtaagtat taaaggggta taaaggtaac
     4861 acttttggac agtaaaggta acaaaagtgg acagtaaggg taacagtttt ggacagcaaa
     4921 ggtaacaaaa gtggacattt gaggtatcac actaggataa gacgttacct tcggttttct
     4981 tgatttggaa ggcgaaaaaa tagaaaatta gattagtaac aaatctttgt tgactgttac
     5041 ctttagttgt gatttaattt aggtaacaag cagaaaaaat tgtttccttt aattagtacc
     5101 agattaacat gattaaagga gcgcaaaaag aagattggag agtaatcaat gtctcaagaa
     5161 gttaccgaaa agattattga tagtgatgta gatttagtat cagaacaaac aaatgcaatt
     5221 gtgcgttata acaatggtct gaatacaaca gctttgcgag gatttaagcc tgttgaaatg
     5281 gatgtgtttt gggctatttt atcaaaaatg aaacgtcaag gaacagcaga agtaactttt
     5341 gatttcaacc tctttcgtta tttggtcaaa tacgacagaa gagaaaagga cagtttttat
     5401 tctgtgttaa aagatttgag tgacaaacta ggtaatttaa cctaccgttt tgaagattca
     5461 gacgtctatg aacagctgtg gctttttcaa aaattccgga tagataaaca gaaggaaaca
     5521 gttacaatta aggcaaatga ggaatttgaa tttatcttaa actcaattgg caaaaacttt
     5581 actcgttttg aacttgataa ttttacaaaa ctaagaagta gctacacaaa agaattgtat
     5641 cgtcatctca tgcaatacag aaataaagag tggcgaaatg gttattgggt agtcaaagtt
     5701 gaagatttta gaagagcatt aagtatacct tcaagttata gaatgacaaa tattgataaa
     5761 agaatactag agcaggcgaa agaagaattt ttagctcctg atgaaaacgg aatacaggta
     5821 tttgaaactt ttgattatga aaaaatctat gcaaagaaag gaagaagagt tgatcggctc
     5881 agattcactt ttagtgagcc agaatcaaac ttgccaacca tttcaatgca taattggtta
     5941 gaagaagatt gagaagaagc aggttgtaat tttaatacaa cctgcttctt taaaaaagta
     6001 tgggtaacac ttttggacaa taaatgcaag aagttttttt gtaagcaagt ttcagtctaa
     6061 ataatatttg aaagaatggc taatctatac aaattttaac aactatgggt aacacttttg
     6121 gacaagtcaa gaaaaaaaac aatttttctt aatttgttga tcgactaaag gtaacacttt
     6181 tggacaacac agaataaaaa gtccggtgat atcagttttg gtaggaaaca tcttaatatc
     6241 tttgtgagat tgaaggtaac atttttggac aagtcaacta tttgttaaaa gttaatatag
     6301 agtaattaga taagtttttt cttttcgtct gataaatgga taatattagt tattttattg
     6361 ctaattagtc tttcatgatg actgaaatag ctaaaataaa tatctcgttg gataattgct
     6421 gatactttaa gggatatcta aatttgctga ttgatttatt tatgttatta tgatattata
     6481 ataacataaa tgtgttacgc ccgaacctca ttttaaaaaa atgaaggggt gagacaatga
     6541 atgctattaa ttggcatgaa tttgttaact atatggagag cttactagat gatctaaaaa
     6601 gttttagtat tgctgatgca gtaaagcatg ggttaccagt tagtacaacg atgaaattac
     6661 gaggattgaa aattccagat gaaattatta agctgagaac taaaacaatt attgaaaccg
     6721 caaaagtcgt tgacaatatg aaccatgaac taaaaggcaa gaaaacaact tacgagaaag
     6781 attttgaacg ttttttaaaa tctgttattg gaaaatatct aggtaaaaaa tctttcagtg
     6841 aattgagaga tgaagagatt gtacctactc caatggttag caaaatccga aaaaacaaag
     6901 ccgaggggaa ttactatctt ggtacaatag caaaaatcat tgattatttg gaaaataaag
     6961 agcaaaagaa aggaacgaag aaaggaagat aatttttgtg ttaaatatta atggggaaga
     7021 atataccgaa atgattcaaa atcccgaacc tagaattacc gaaaaaacgt ctgattgttt
     7081 gctcttttat gaaagggggc agacaaacta atggaaaaaa acgtcaattc aacatctacc
     7141 aaagatcaat acagtattat tggagggttg cttggtgata aggataagag aacacgtcac
     7201 ggattacgca ttatgcttgc aacttttcta ggagtgcttt ttttattaac aggcttgggt
     7261 agctacattt atcaattagg aaactataat tattatctcg tgaacaatac aacacctata
     7321 ggctcttcat tggagtttgc tcgatcagga gcagagatca ctatttcaga tgtgtggaca
     7381 gatcaaaaca gagatgtaac agtagtaaaa ctcggttact cgcaaaacgc tagaaagttg
     7441 ttatctacgc aaggaaaaaa ctacaactta tatatggcaa cgaaagaaaa agatcgtcct
     7501 aaaaagcttg aaatgagcta tgggatgtta agtactgatg gggatgcttt cttattccta
     7561 aaaggtaaat tagatgaaag agcttatcaa gtttttattg caaaccaagt atcactaatt
     7621 tctggtgatg atacagctga cgatgatacc aaagtcaatg tgaatgaagc agattcagtt
     7681 acaaaagcac tttctcaata ttctatgaat gatgttaata atgaaggagt aatttctttt
     7741 gggaaaaaag caaaacctgt taatactcct gataatatca atttcagaat aaatccatat
     7801 tcagattcaa ctaatgtcta tcaaggaagc tttcttacca aagatggcga tattgattat
     7861 tcaaaagttg tagcagttac aagtaagaat aatgttgtaa gtaagttaga agatcagtta
     7921 aatgcttcaa atactaacat caagaaaatt gaagctacaa aagctgagta tctggaacgt
     7981 ttacaacaaa atcctgacga taaagattcg caagaaagta ttcgtcaact agacagtcga
     8041 atggagaccg aaattaaagc aatcaatgat acacaaaaat tgattgatca atacaaaaat
     8101 gctgacttta acaaagattc ttttggagac atgaagacga catttaagta cctaactctg
     8161 actgactaga agtaaattat tcttatttta gaggaagtga acacaaacag atggaaaata
     8221 gtaccgagtt ttttaagaat attcaaactg aatttaatca tgctctcaca ccaatgaaac
     8281 caccgaaaca taagttagtg ggacgtgatg acgaactcca tgagttgtca ctgatcttga
     8341 atagaccgga aacaccgcaa gctatcttgt taggtgaagc cggaacaggt aaaacagcac
     8401 ttgcaagtga atgggctgaa agacagcgac aagatggaaa gaaagtacaa gtatacaaat
     8461 tatcaatcgg acaattgcgt gttgggggag atgatgtcct ccaaacacgt ttagaaacat
     8521 taattccaag attgaaagaa tatgagcgaa ttttaaaatc tgaggacaaa gaaaatgaat
     8581 ttgtcctctt cattgacgag tttcataaag ttgttagctc ttttggtagt ggcaataaaa
     8641 ttgggggtga ttcgctaaaa gaaccgttag caaatgaatc acttcaattg attaccgcta
     8701 ctacaagaaa agaatatgaa aactatatca aaagagatga tgccttaggg agacggttac
     8761 aaccgttaga attgaatgaa ctaaaaagcg atgttgtcgt cgatattttg agacaatggc
     8821 taaatacatc ctctgttaaa gatgatcctc aggcttcttt taaattatcg caaaggattc
     8881 ctgatgaatt acttcaagaa atcgtacaag caaacaagct ttatcgtgag gatttgtccg
     8941 aaccggctaa atcaattgcc gtaatggagg atttgattgc aaaagatcga gtagacggaa
     9001 atgcttataa tcgagaaagt ctattgcaaa tatttggtaa gtataatatt cagctagatt
     9061 ctgattttga tatggaaaaa cttcaagagg aattaaagtc cataaaaggg caaccgttag
     9121 cagtttatac tataacaatg ttgtttaaac gtatgaagct tggatttgat cgttttaaag
     9181 gtcgaaagca accaaaagca actgctctgt ttacaggatc aacaggggtc ggtaaaactg
     9241 caatggctaa agccgtggca agaggacttt atggcgatga aaataaagtt ctcttagtta
     9301 atatgccgga ctacaaaaca aaagatgatg caatgcgttt tagacgactc ataggtactc
     9361 atgtaaggca tgatccttct gcgattattg tgtgtgatga atatgagaaa gctggtgtag
     9421 aaacactcga tgccttttta tcaattttag atgaaggtag gataagtttt actgaaagag
     9481 gatgggatga tcgagaagtt cacgacactg tttcattgtc aaatacaatt ttatttgcga
     9541 caacaaatgc aggtgcggag atgttcgatt cgttaaacag atatagctca ctatctcgtg
     9601 ttacaaacaa ggaaaattct aatgaatata gcactgaact aaaaagagaa taccgaaaag
     9661 tcgcatcagg gatcaaacaa gccttgattg gtgaaggtct aaagcctgaa gttattaacc
     9721 gatttggacg aatagttcca ttttgggctt tatccgaaca aactcttctg gaaattgctt
     9781 ataatgaaat gttgaagagt gctgaattag tacgaaaaca tttaaatata gatttacgtc
     9841 ttccacctag gaaagaaaga gtatatggtc aaaattttta tgcagacgat ctctccatgc
     9901 acttagtttt tgacatggct tcacaagagg attctaaatc agggggtgca aggaatatct
     9961 tgcatttagt tgaatctgag tttgaagaag cgattactga agcaataatt acttatccaa
    10021 acgcaaaaat attcgatgtt aagttgagtg aagccggagc gtttgaagga aaaggtgatt
    10081 ctaatgctga aattattgca aagccttccg ttgctgaaag taaagaaaac aagattgtat
    10141 tttcgattgg aggggcgtag ctatgaaatt caagaaatat ttactacaaa accttagaat
    10201 aaaacgatgg gtcgtaagag gtctagttat tggaggagta ttagcagtta ctttaggcgc
    10261 tccttttgga atgaatgatc caactcctga caatcaagat agcaaacaag aaacagttat
    10321 aaataagaac gttgcatatg cggaaagtaa accggcagaa caaggaatgt ggacactata
    10381 tgctcgtatc ctaatgggag ataaagaagt taatgatagt gccgctaaaa ctggacaaat
    10441 gaatttgtta ggacaaggtg gtatcagtgc cgatttccct tattctgaca tttctaaaca
    10501 aggtaattcg attgcagggg gtaatgcagg tactcaacta gcatctactt tagcaactta
    10561 tagttactac ggatatattg aaacagtctc aggaaatgct attgccagtc aagcatcatc
    10621 cattctagga agtattggta ggacgattgg tagttttatc ggatcaatta gtttaatcct
    10681 ttctaatatt gctgaatcca taaatgaact ccttggaaaa tttttcatga gtatgaatat
    10741 attttctctt atggggtggg gagaaggtgg atcggatgta tctaatccgg tatctaaagc
    10801 attaactagc ttgcttaaca atttgggatt aaataatgaa ttatttaaag cgatagcaag
    10861 cttatctatg ctctttttca cagcaatact aggttatcaa ggaatgaaat tattgtcgaa
    10921 agatggtgtt aactcgaagg gattatcacc agtacttgaa aaatggattg ttcgtatttt
    10981 tgtattgttt atattcttac cagtggcagg atcgctattg ggagtttata tcaacggcat
    11041 ccagtcagca aaaaaagatg gagatatacc aacaaatgtt gtttctgact acatcattaa
    11101 cacaagactt tgggcttcta ctcaaaatct tgctccatca tctgatactg gtggtgatgt
    11161 acctgacgga aagcctacat ccgaaatcaa gttcgttgat acagattatg aaccatctaa
    11221 aaaatctgca atgataagag cgatcaatac tagcagttat gccagacaaa ataatgatct
    11281 agattctaat tcagtcggta tggctctatt aaaagaatgg tcatcgaatg atactttcaa
    11341 tattaatact tatgccggtg atgttaatgt cgccgaaaat gatgcttata tgggaagagc
    11401 gcctgctgtt tggacaggct ataatttagg ggatggaaaa gaaggaagtg caattaagaa
    11461 accaaccaat ttaaatggat atatttggtc agcaaatcaa aatgtgacag acgacaatcg
    11521 caagcctgat ggaaaaggaa cagcgcataa ctttgatgct actcaacctt ggggcgttgc
    11581 taacaatgca acttttagca cacagtcggt agctttattg ctccaatcgg cacttgatac
    11641 atcgggagct aagttttatg cgtataatat cgctccttca ggaattcaag gcagtgctaa
    11701 gaatatgtca acagtaaaaa cagaatggcg tacagcatcc ttagttggtc atggagctat
    11761 gggtaagttt ggctcgtggc tagcgatgaa cgctgaatca atcgttttag cagttcttta
    11821 cttagcatgt gcttgggcga tgttcaatgt gaatatcgtg aaagcattta aacgtgaatt
    11881 tattcggatt tgggaagtag ttctaacagg atcacttgca tcaggagcag gtgtgatggc
    11941 aatttcatta ggtgttgtta caacatctct gatcggacta caattaccta tctttctgat
    12001 tacttttatt cgtgcatttg gtgatactgc tgtgggattc attcctaata cattagttgg
    12061 cgatggcgta gcaaatattg ttcgatctgc tatgatttta ggtgtaggat atcttttagt
    12121 ctttaaaaaa ggcgaaaaag taaataattt gattaatatg cttattggct taccacttga
    12181 agtagcgttt gggtttgatg aacgtgctaa gttattattt ggtaatagaa atgatgctgt
    12241 gcgagttgtg gacggcgttt ctaaagcaat caagaaaagc caaggagcta aaaatcccta
    12301 ttatgatggt ggcaacagct cagtaaaaga tatggttgca ggaggtcttg gagcagttgg
    12361 agcgcaaaaa atggctgaac gtttgaacaa tatgggagat agcaatgctg atgctaagtc
    12421 cattgctgat aaagtaattc ctgaaacacc agaaggaatg tcattcaacg gtggcggagt
    12481 tggttccaca atcaataata cctctttcaa agatttagtt cgtcaagatc aactacgtaa
    12541 taactcccaa caatatattg atcatttagg acgtatggtt gatgaaaacg gaaactttgt
    12601 cgatgaaaat ggtgaaatat taaaagatag taaatacggt aaagatgcta aaggcaactt
    12661 tattgacgaa aaagggcgtt tggtcgatga agatggaaac cttatagata ctaatggtaa
    12721 accattggaa gatagtgaag atggtaatat tggtatggat agcgaaggac gtttaattga
    12781 tgaaaaaggt cgcttagtcg atgaagatgg aaatcttgtt gacgaaaatg gacgcaggtt
    12841 gctaaatggt gattttggtc atgatagtaa cggacgttta gttgacgaaa aaggtcgttt
    12901 agttgatgaa gatggaaatc ttgttgatga gtttggtaat aaacttggta aacacagcga
    12961 tggatataat gtgaatggag atttagtcga tgaggatggg aatatcattg agacgagaga
    13021 tgcgcttgtg gcagatcgaa atagagaact cgaacaacaa cggcatacgc caattgctgt
    13081 tgcaattcct gagaatgaag aagacgagat tactttagat caaactcatc aagaactgag
    13141 gaatgaaatg agtggtgagg acgtgtcaga cagagtgcat cgagaattag gagatggagc
    13201 atctggtgaa agtcagtcaa gagagcttaa cagcaacttt gataatagtg ctattcggaa
    13261 tgacacagta cttaaagaca gagaatcgct aacaaaagaa acggataaag aacaatcaga
    13321 tgtgaagaaa aagttcattc ctactcctaa tgttaatcaa acagataaat atgaaacaaa
    13381 tattggatca ggaatgtcga aagacaatca atcaattaga gaacctgaac tgacaaataa
    13441 aaataataga acaaataatt ctaaatctac ttcagataat tcagggaaat ctaatcaatc
    13501 aaccaagaaa acatcaacag ctaaatcagc cgttgaatat ctatcaaaaa ctggtaaaaa
    13561 tgtttacacc tcagcagata aagctttagc aaaagctcca aaaggtagtc tagcagggat
    13621 aacctatgaa gctaaaaacg aagctattaa aataggtaaa ggaacggcta aggttgccaa
    13681 ggctactgct acaggagcat ccaaaactgg taaagtagtg aagaaatcta ccatgtcagt
    13741 caaggactat attgtaaaat acggtaatca aaaatcaact gaaaataagt ctgaagacga
    13801 gtaaaaagaa ggagcattga tttgctcctt ctttttactt tgatttctaa ctgaagatgt
    13861 caaggaaaat ttctatttat gcaaaaaaat tattttaaat tgtctataaa tgaattaaat
    13921 atttactgtt caaagttgta taattaaaaa gggcttatgc tggtaaagaa cagaattatt
    13981 atctaaacaa aaacttgata aaccagcatt tttagccaat aaaaaaagtg aaataattta
    14041 atggaaaatc tcttttaatc atcgaaaatt tatgttatta tgataacata aagatgtaaa
    14101 tatcgagacg tctttttctt agttagatca ttgacaagtg aacaaataag atttgtcgat
    14161 gatataatta acttattaaa aatattaaaa aggaggtttt tatatgttcg attctattaa
    14221 aaatttggtt agcggaatga cgcctgatat gaaaacttta gtacaagttg ttttgtattt
    14281 cttggcattc gttttttcgt tgaaaccagg attcactgca atgaaatttt ttggtgatgc
    14341 aaaatgggga gatggattaa aatatgttgg ggcgattatt gcaatcttta ttattcctgt
    14401 aattacaacc gttatgcttt acacaatggg tactaaaaca ggtaatgatt tgaaccaaaa
    14461 agctaatatc atatcagcac ttattccagt tgttggtatc tatctagttt caaaatttcc
    14521 aaaaaatgaa ctgttaggtt aaaagtgaat tgaacaaatt tggataatat gccgatttgt
    14581 tcaatttact ttttaccaaa aaactacgct ccaagcgtag tttagaagtg agggaagaaa
    14641 gtaaatgtaa gatggttttt gatagagaaa aaatgttagc tcacgccaac gaagtattaa
    14701 tgagcagtct gaaaggtacc gaactggcaa agataatgaa tatgaatgtt aaccagttct
    14761 atgattatcg aaatgggagt aaaaagattg aaaaagccag acttgaaacg cttattaaat
    14821 tcgaaaaagc atatgtatat atgctagata aacagaaaag aactattgac tgaaaaaagg
    14881 agttttacaa taatggcaaa attcaaagat attaccgaat ctattaatta tgtggcagaa
    14941 acagttcctg tagttgcact ctctttgaag ccacaattac tcgaagtgta caaaaaagaa
    15001 gaaaaagaag cgaaggcaaa aaaagctaaa gcaaaggtat ggaaagaatt cgatgcgttg
    15061 ctgaccaaag atttaccaaa gaaaaattat atggtaattg aagatgtaca aaagctcaac
    15121 agtgagggag ttatagatga ggatgatgct attctcgatt ctgagaagta tgtcgttcta
    15181 ttgaagtcaa gtttgaatta tgtagctgat ctaccagaaa aatttaaaga gaagctatta
    15241 gcaggtgtcc gttttatgga tagtagtggt caattattag ttattccgga tgatcctaat
    15301 gtgtttgaat cagaagttga agaagctaca gacttctata gcgcagtaga gcaattagaa
    15361 gtagctacta atggattaga acgaattgct gagaccttca actcttctga agaagttgaa
    15421 tttgaagccg tagcagaaaa tgtaccagat gaaaaagaag ctctttttgt ggatgataac
    15481 aaaccaaccg aagcagaaaa ccaagaagta agcatagaag aaacacaaat acaaacagaa
    15541 acctaccaac aaccaaaaat tgatgaaaaa cccgaatccc aaagtgacaa agaaaattat
    15601 gtttatgaag ctgaaaaaat cacatctgaa agtgatgtac cgaaaaactt agatgctgat
    15661 tttttagaaa aaaattttga acagatcatt gctgacaaat ttcaacctgt agttttagat
    15721 gagattaatg accctgtcga tttaaacgag gagatgctat ccatacctga gttgaaacca
    15781 ttgctgtttt tagctaaaga gagtatgaac gctcgcatct ctattgctaa taataaaatc
    15841 aacagtgaat taaataagac gaaggagcaa ttgatcctca aaggaaagca aatgttagct
    15901 gatgagcttt tgcggataga ggaagcaacc tcattaaatg gtcaaaatca gtttacaaat
    15961 acactatcgg ttgctaaaga gagctacgaa gcagaagcat ctaaaattga tgatttagtg
    16021 atggaaaaat ccaatatcat tcgtaaagat tatgatatgg cgaaacaggc ttatttggat
    16081 gctgaactaa agaaactaga gcaacggtat gatgctgagc atttaccaga agttaaacaa
    16141 caagaagatg aatatagaaa agcaactgaa gaaagtcttg atcaacagta ccaagaagca
    16201 attgaatcta ttaaaaacga tgcattgaaa caaagccaag aagcaaaaga agttgtggtg
    16261 gaacgaatta tcaattcatt gaaaccagaa attgaaaaat cattaacaga cgtgcaagaa
    16321 aaaactttag agattgtcga aaaaacaaag tcagaaaatg aagctgagtt ccatgataca
    16381 aaagagaaaa caacagatgt ggttgtcaaa tattttgcga acaaagaaga agttgaaaag
    16441 caggtagcta acaacacgaa agaattagaa acaaaacttg tagagcaaca aaaaactatc
    16501 gaagaatata aagatcgaat gaaagaggtc aaagatttag cagagggtta taaattaaat
    16561 agcgaaaagt tggcagaaca aagtgaagct ttaagagaag acttatctcg ttcccgaagt
    16621 gatctagctc aaatggcaga aaaaacgaaa tcattagcag ttgctacaga aaatcaatca
    16681 agttcaaaac gtgattttac aagtaaaaag atggctacta tcatttctgt cgctgcaatg
    16741 gcttgcggaa cgatcttggg atcgtcctac tttattagtc ataataatca acaagcacag
    16801 gctcaaactc aaacatctat ttcgagagtg gaaaattcga ttgggacaaa tacgaactct
    16861 gcttcaacaa atagtagcta tgaagcaaca ccaactacag agttcaaagt tgggaaagat
    16921 gttgtcatca gtattgatgg gaaaacaact aaggctgaag tgatttctat tgatggagaa
    16981 aaagcagttg tgaaaactcc agacggaaat cagtacaaaa ttcaagctca cttatctgat
    17041 aataactaag gagggctatt atgcgagaag aaaaaatagt ttatccagta attcaaaaca
    17101 atcaagaaaa catgacctct tctcagcact tacaggaagc acagctcgtg tatcaggtaa
    17161 atgaactacc acaaacgaac gagacaatga gcaagtcaat atctttaatt ggcttgctta
    17221 ttgttgcaat agtgataact gtaggtgttt accgtaagtt gaacgtgaaa ggaagtattt
    17281 ctgatggcag ataactcaaa ctatggattt aaacataact tacatcaaaa accttctgaa
    17341 acaattgtca gagttgaatg gcttgaaaag catgtcaata gcacctattc tgttacagca
    17401 aaaagattaa ttgtatcagc tatagttgtt gcagtcgttg ttcttggatc aattgtttac
    17461 ttagtttctg atacgattaa actgaaattt atttttgttc caacgttatt aatgataatt
    17521 ggatttctta ttctaattag ccagtatatg tttattgatc catctagtgg ttcattgatt
    17581 tctggttcta ttagactgtt caaatcagta gttaattcag caagagtatc tatggggaaa
    17641 aagaaaccat ttcgaggcac agagatttta aaagtgaaac aagatggaac aatcgtatat
    17701 cgagatggga gtgtagcatt aatgttcaat ctagatgggg ctacatcact aaccgctttt
    17761 ccagaagaag ttatggctca ggaatcgaaa tcaatacaat atcaaaatac cagagatcgt
    17821 tccttagctg aaatcaaaat cacttcatct caacctcaga atacggaaag acaaactcga
    17881 aatatgaaaa tacaaaaacg aaatgcaagg agtaacaaag cagtctttga cataatagat
    17941 cagcaagaac gatttgtatc agaatatatt gatggtgtga aaacaacagt aatccaatat
    18001 cttatcttga aatctcaatc taagatggta ttggatgaag gtgtggaacg tttagaaaac
    18061 tatacgagta gaggacttta ttatagtgtc actcagttgt cagctaaaca aacaaaagaa
    18121 atattatctg atatttatgc actgaaatag aggtgagaga ttaaatggaa attatgggta
    18181 gatcacaact attaaatttc aaaaaaaaga gaaaaactaa aaaagaacaa aaaactagaa
    18241 attcaaaggt agtgaataat acctttgtta gtgatttaac atctttagtt ggttatcaat
    18301 ttacaccatc ttacattagg acaggtaagc ggtggggaac aattgtgaaa ttagtgaata
    18361 cttatggaat gaatagaaat tatggatttg gttggatggt taaagctatt cctgaagtga
    18421 gagtagaagg ggtaaaagcc tatctaattg aagcagataa gccagtgtca caaaaagatc
    18481 aacaagaaat aatgcacgag aatgttgggg gatcaacgaa tagttatgaa aataatttag
    18541 aaggaaaaaa ttcagatgct gaaattagca aaactgttat gcggattatg gatttgaata
    18601 cagcatcttt actagacagc atggcaaata aaattattga ttggaagcta gaaatcctat
    18661 tagtatccga ttctcctaat aaaatcagcg aacagctaat taaactccaa cggttgtatg
    18721 atgaaaaaat gtatggatta aagcttatgt catatggagg agatcaacgt gatctgtttg
    18781 atagactttt aagtgaacca atcgggaatg tctatgagta tacgtctatg agttcggatt
    18841 ttgcaggaaa tgatcatgca attcgcaaag gtttagatga tgcgaatgga tgggctatcg
    18901 gcactatgtc tactagctac gcaagtggtg tagcttttat ggcactggat gattctttta
    18961 caagtaaagt tttagtggca tcacacaaaa acgctacaat taaaggctat gatgaaaaaa
    19021 tctcagcatc atcactttgg gggcaattga ttgctaataa tgcaatggca aatcataaac
    19081 gaacttttca tattgtctta aataaatttg attactttgg tgatccaact ggtagaacat
    19141 ttgttgccaa accttcgtta aacagtgtaa tagaaaaggt taatttggct gaagaaggtc
    19201 ttaactcttt ggagcttttt ggtaatcaaa aggacattgg tgatttatat agacggaatc
    19261 tcgataaact aactcagatt ttttatttgt tagcaggcag aacattagat tctgcaacta
    19321 gagttttatt gaaaaaagaa ttaggtaact tttatataag tcagaaactt tgggatatta
    19381 attatgaaga acgttcaaaa cttttggtga acagtagtga tacaatgcca gttcttgggg
    19441 attttattag ctatttgact aatgctgaaa caaaagccaa aaaatctggt actgaaaaag
    19501 aagtcgatcg tattattgta ttaaaagaca cattgagaac agcattagac agcttctcta
    19561 ggatgtttaa tcaaagaacg acactgaaag aaatcggtaa aagtgacaaa ttacagtttt
    19621 actataactt aggtgatcta agagcagaaa ccaatgttat ggaagctcaa tttttaaata
    19681 cctttgattt catcacatca caagcaaaaa aagatgatat tgttatgatt cacggcttcg
    19741 ataagttatc tattgaaacc gttgggctaa ttaaaaaaag attaacaatg cttgaagaag
    19801 atggaattcg tcttgccttt ctatttgata caatcggttc aggagaagac aatgaataca
    19861 aagaagatat tgaaacagct gatatattca atacagatgg aaaaatttac caaagttttg
    19921 aaagtgattt taattacact atcattggaa agatgaccag tggagagtta atgaagtatg
    19981 aaaaaaaagt tgctcctgct agaaaaggag aagatgatca aaaactttct gttgatttga
    20041 gacagtattt gacaacagga agaagtaata aatttcagat caggagacca gtggatctga
    20101 ctaataattt tgttattgga gagtttattc tttaagtttt atctttgatt ttgagtgtat
    20161 aaggaaagga taagaaaaaa catgtatatt tgagactaaa aacagtctta gagaacatgg
    20221 tacgttttgt gaaaagagaa ctatagaaat ttcataaagt gaaaccttac tgagtgaaat
    20281 aattatagtg gaggttaaat aatgaaacca aacataaaaa ttttagatcg aatatttcta
    20341 ggtagagaca cagaagtaat cctaattcaa catgaagaag gatttgaagt tagtataggt
    20401 attcagaaac ttcaaaaacc tcattattgt aatcagttgt ataaaaactt tactgatgaa
    20461 gaaaaagcga gagttttttt taaaacaatt tcctaggaaa aatagttaac ataaaaggaa
    20521 tgaattgtta tgaaacaata tttaaatgaa tggaaagcga tagaggggtc attagtaacg
    20581 caaagaataa aggaattacc tgattgctta gagaaggatc atttgtttca aatacgagaa
    20641 atgttgaaaa aagaacagtt tgatccagac caatttttag tagtcgagta tccaactatt
    20701 ggagtgtact gttgtaacca tatcaatgat ggaaaatatt ttattattca ggagtatgga
    20761 ggacagttaa ctcctttcta tactacatgg gaaatgagtg aagaaggaat caataatttt
    20821 ccatgtgaaa gcatcgaaga ttcaatttca cacaccgaat gttaattgat tataactata
    20881 aaaaaggatt gaacattatg ttcaatcctt ttttcaatga aatgttttcc gccatttcat
    20941 tgtttggtgc gatttctatt ttattatatc aaaataaaga gagattctaa agaaagaaga
    21001 gggaaatata taaatcaagc gacagtatat ttattattaa atgctattgt gagaatataa
    21061 ctaattagtt taatttgaaa gtaaatatac gctataaaag tgcatcttgt gttataaaaa
    21121 attattttat tttgtgaaca taatttaaca attctttctc ggaattgatt tcttttctta
    21181 aatagtcttc agacttatta ataatattat ctattatgtc tagatacata tcacttttcg
    21241 attcagctaa ttcatacagt ttttttaatt ctttatcctt taattgaaaa ccctgatcca
    21301 tagtcactgc atttggatat agaagcccac tataatgttt acgtaaattc acttggaatt
    21361 tctgattata aaagtctaac agattagaaa taaaaggcgg aaaattactg tttttgtaaa
    21421 attgattatt tccatttagg ttgtatagta atttgttaaa ttgctcctga tttaatttat
    21481 tgtaaatcca tggtaatact tccatatcct tatctgctaa cctgaatgaa ctagcatcgg
    21541 taagatgttc gatcataaaa tctatgaaca tctctttttc accaaaatat aaatattgat
    21601 tatatattgt ttctaatgtt gaagatggta gcgaagaaac tctcattttt tgctggagta
    21661 taggatcaac tagactattt aagtgatttt gtaattcttc attgttgttt cctagaactg
    21721 caagggaacg taaacggtag taatattcta tttctgttag tacttgttgt ttcggtttaa
    21781 aatcatttat tgatatttgt aaattttgaa ttgtctcgtc tgaaaaatca tgttgtaatt
    21841 ctggatgggt agctataaaa gtaagtaaaa agaacccttt tgtatacgtg atctgttgat
    21901 tttcaccaag tgatttatta atttcctctc ttgattcagc tgtgaaccta tactttgcaa
    21961 tttctttttt tagaatattt aaatagaatt ctttattatc aaaaattagg gcattaagta
    22021 gtctgaaagt actttcccta tctctatctg cttcctcatt tccataagat aattgaaaaa
    22081 caaacttaaa ggctgtctta cataggtttt ctttggcttt ttcgcttagt ttatttagat
    22141 agctattttt tagtgcagag tgaaattctt caggtgtaac agacatagca tgtatgcgac
    22201 ttgcatattg tataatttcg atcgttactt tttttcctaa tattggattt tttaggaata
    22261 taatttcata ggacttccgt ataacatcct gacaagtttc ttttgttata tctcttaatt
    22321 cccaagtatt accgttgtaa gtgactacag gatgagctga ataatttctt tgatccttaa
    22381 tatattttaa atcctcatat tcatagctat caattagctc agttgaacta taagcttttt
    22441 ctagtagttc catttcccat tttggagagt ttggatcact ttttcttagt ctagctatat
    22501 cttctaaaat tttcttagaa gtattatcct cgtaaatatt tgctaaatca gataatttat
    22561 caattaaatc taaaataagc gcactgtaat tgacagctat tgcagaacga taatttccct
    22621 gataaaaact acttctaact tcttctaata atggaataat tctaggattt ttaacttttt
    22681 ttatttcatc atctaatata aacatataaa tctccttttt ttatttgtat gacaagttta
    22741 ccataaaaaa aaattaaaaa gcgagacata tttatgttgt tgtgttatca taataacata
    22801 aatatgtaga gtgagatgat gaaaaaatgg atttgaaaca atcgttttac atctggttat
    22861 atcctgataa tcctatgtca ataatggctg atatttttgt agttagcatc ttattgtatt
    22921 tattttatct aattttcttt cacaatttaa tcgggtacat gattcttgat tggaaaggaa
    22981 tgttcgatat agaaagaaaa ccatttgcta acaatcaaac ctataccgtc ttcgaaagtg
    23041 gagattggga agaaaatgaa gttgatgtga tccatgaaat tggattagct ttatcaggaa
    23101 tacaagatgc tgatgttttg gtatcaatga caacggatac tggatacaac gcttatgagg
    23161 tattttatct tatgtatttt atatggcgtg atggtcaaga aattatcaat ggtcgtgctg
    23221 aaaaaacaat caattctaat actgaaattt ctttttgtat gggtgaaggg gaaatgattg
    23281 tatataaaga tgggtcagca aacattaaaa cagcctttgg tgatattgaa acaagtatta
    23341 aggaacttag ggaatatgtg cagaacccat ataattttca atatttagca ggaagaataa
    23401 atttagttta ggagtaaaaa tatgaaaaaa attataaatc aaccaacaaa tcacttgata
    23461 attgaaggtg ttattttcgt aacaatgtat tttactgtag gaaatggcat ctcaaataca
    23521 atcattcaaa aaaatccaca atacctatta tttagtcttt atgcttggat tgtacaaatg
    23581 gtttgggttt ccatattgat gctgttgttt tattggatag tacgggttat tcttttattg
    23641 ttaaacaaaa gaaaaaagga taggtgaata aaatgaaatc aactaaattg aaggttgtat
    23701 tgttatcgac agcagcatta ataaccctta cagcatgtgg tacaactaat agtgctaata
    23761 ataacaaaga cacaaagtct agtagtacga tggttactaa aaacagcact acatcggcat
    23821 caacaacaat gtcttcaatg actgaaaata atgctgaaaa gtatcaaaaa tacgaagaca
    23881 ttaataaagg tcaaaagccg gatgaagttt ataacaactt tattaataca gtaaaaaatg
    23941 atgaaaatta tgggaatgat ccattgcaaa ataaaaatct gactatcgtt ctttacaaac
    24001 ctgattgtcc agattgtcaa aaagcagaat caaaaatcat gcaagaaata aaagaaggat
    24061 ttagttatgc tccaacatta atgcatgata ttaccataaa taaaccagct tacgagtatt
    24121 acgcagttgc tgtagatatt actgatggtg tacctgagtg ggtacaaaac attcctggtc
    24181 tggctgatga gaacaatcaa tatcatacgc caacagttgc agtagtaaca ccaaagaaag
    24241 ttaacggcac actatactgg gatgtaatgg gaatgacaac aaatacaact gatgaaatga
    24301 ttgaaagtac aactaaatat tatatggatt tgggagcata taaattatat caacaatcag
    24361 agcctgttat ggacgatcaa tccaaagtta aatagatctt tagggcgtaa gccctatttt
    24421 tttaaaatta agtgataaca aataaatgat ttctgaaaca aacgttctta ctcaactact
    24481 cgaaacacac aagctaatta actagattat aaaggagaat gcgtaatgaa agaaatcagt
    24541 gcaaatgaag ctcatattcc gcaaaagaat caggttggta tatataaggt gcttcatggt
    24601 gaaggaattt ataaaataca gttacttacc ggttacaata atggtgcaaa tgggaagtgg
    24661 ttaaaggcaa caagtattcg tgaacttaga agtgaagtag aaaaaattag gcttggtctc
    24721 acaagtgaag aaatagctaa actgaaggga aataattctt ttgtaattga aaaagaaaag
    24781 gaaaaacatc tactttatcg tgagctgaaa attgaagaat actatgaaat tgatttacct
    24841 gaaaaattaa gaattattga tcaacgttca gaagaaaaat ggaaaactga aatatatcca
    24901 agcttaaatg actacaagac atcaggaaag cccatgtata actacacagg ctttttaacc
    24961 tacacacaat acaaatatgc ctttcgcaaa ttaaaaggca ttaaacaagc tgaaattgcc
    25021 ctgtatgaga tgaaaaaagt ggatagattg aaattgattc aagatttagt gcaaaataca
    25081 tggaatcaag cgattgtcga gctagaaaaa gaaaatggga aaattttaac taataaatat
    25141 ctgatcgtaa atgaaattga agaattaatt ggtttatttt catgtgaaaa aggtgaagtc
    25201 ttcttgaagt catcaccaac ctattcaatt ttggacagtc cgtatcagtt tatacaggca
    25261 aggagatgag ttagatgtct agcttgaaag atgactatat ccaagcaaaa caagagtatg
    25321 aaaaaataca gatgttgaaa tctgagaaag tcgaattatt tttaacacaa aaattagcag
    25381 aggatcatat tgctaactta gatgctaaaa ctttagagga atatgatcgg cagtttaatc
    25441 agatacatca caatatcatt ttgcttgttg agcaggcaaa gcgaaggttt gaaaaagcgg
    25501 aatatgatct attagatcaa ggaattgtat gcttggataa gggattgcga agatattatg
    25561 gtgcagagtt aaaaaagcaa aatgtagata gaaatgcact aaaacatgaa atcttagaat
    25621 tgataatgca acgaatttga agggatagaa agcaaatgtc acaaaacatt agtgctgtaa
    25681 aacaattaat agaagaagat ttagagacca cagttcagag attagcggaa cgaatccatt
    25741 ttgcaaatta tcaaaccatc tctaactggt caagcaggaa cagaaatccg gaagaaattc
    25801 caatgaaatt tttttatcga atcggaaaag aatatggtgt tgatccgata aaaattttcg
    25861 agaagaccat gatttattac tatcatggaa cacctaacga agtgttagaa catcttttgg
    25921 aagaggcaaa agaaaaatca tcaatattag agctatcgca aaatgacaaa gttattcaag
    25981 tgaaatgcgt tgatccaaca ggaaagatta ttgtcggttt tgatattgaa agaaaatcca
    26041 aaatagattt tattacacaa ttaaaaaaga taaataattt agatattcta acgcttagac
    26101 cagatcatga acagttgttt atgagtttta aaaaacaatt gaaagaaata caaaaaagat
    26161 ataacaaggt gtttcctaat gcgtgaaaag ctcaaagaaa tcagagtttt ttggataatt
    26221 aacaaagaat gtatcatctt atatactctt ggatttctta gcatgatctt gttttggtgg
    26281 ttatatttaa aacagtaatt ggagagggtc aagaggattt ttatcaagat aaggatgtgg
    26341 aaactgcatg tggaataata gattaaaaac aggattgtta ttaatagtta tttcctgcgc
    26401 tatgatgatt ggtatgagga tacaaaggga acaatcctat tttgaagttt ctgctaacaa
    26461 tgtaatagaa agatgccatt atggtcaaca tttttggact ggggaagtca gagaaaatat
    26521 agatcgtgaa tatgttcaaa gaattgtttg ggatgcatat tctatcaaag attatcctaa
    26581 aagtttaacg agtagattat tttacagtga aaaagataat caaaaactct cagatttgat
    26641 gatgaaaaaa gtatgactat tattacgaaa gagaaacaga gtgctatatt cgaggggaaa
    26701 aagcctacaa caaaagtgac tattggggtg acaaggcgaa gcgattaact gcaaatttta
    26761 gagtgtatga atcagagttt ataaacttga cctttcttaa tagtgttttt attgattatg
    26821 taattactac gaaacgcatt ggaagttatt caagtaccta cgcatcctta gttccaatgt
    26881 taaactcgat gagccgtttc ttaaaagaaa gagaaaagaa agagctacag tatatcaaag
    26941 aaatcatgcc ggattttgaa atgcacgaat ggcaaattgc tttatcagat tggaaattgg
    27001 cgaataaggt tcgtagaata acaccttttc aagccaagcg ctttttgaga gctatgaaat
    27061 ttgtgcaata aaaaaagcca ccagacaagt tgatggagac taacacaatt accaacagtt
    27121 gtaattgtgt tagcttacaa attaattatg gtgtttagtc aatcccaaat tgtgaaatgg
    27181 caccaataat tggaaaggaa aaatgaaatg tctgaagatt tacgagagct gaagttacga
    27241 aagaagcagt atgatcagta tttttccggt ttgttgaata aacatattga agaaggactt
    27301 gatcatgatt ttggaaaaga tgaatattgt ttatattgcg aacgttacaa tgaaaaaatg
    27361 tctgaatatc tacagctgaa aaaagagata cagaaattag agaaaaaagt acatgcttgg
    27421 aagtataaag attccatgtc tggttatgtt gaatctcaaa ataagccaag aaaaaagaag
    27481 caagcgtatg ttctaaagat ggtaaatggg gaagagttaa ttgtattagc gtacagtgaa
    27541 gaagaggttc gagaactcgt tgaaacacct gtcaggaaaa aggttgaaac aataaatgag
    27601 atagacgaca gccaatacaa tagcatctat attgaaacaa gtcatggtgg atataagaaa
    27661 ttagcaactt tccttagaag ttctaaagtt gggataattt ctaaaaaaga cataagtgct
    27721 gttaccgata gtttttgtac tttatctgtt cctaaagctt atttccaata cgatagtaaa
    27781 agatttgaac gcttagagaa attctgtaga ccgtatactg tattgaaaac taaaaaattt
    27841 aataaaacta aaagagtttc tatacagtta ccgactggtc aaatctcatt aggtgaaaaa
    27901 gactatctga taaaagtagg aaaagagtta attataaaag taccggaaca cttatttaaa
    27961 gaaaattttg agatacgtga aaccttggta tggaaagaag aaaaatagtt tggaagacat
    28021 agaatggtat aaggggaaat tgaaatggca gtaaataaaa tattgcaggt actaatcata
    28081 gacgaatttg gcggaggaga atggtttgat gtgcagtcca tagactttga agaagaagtc
    28141 gtagttatag aaaatattta cggcgaagta tattcatatt cgcttgaagg tgagaatatt
    28201 gaggatttac ggttgattgg tgagtagaaa aaaacagaaa tatcaaaatg gagataaaaa
    28261 ttaatagcat gtaaagatag tcatgcttgg gttaaaaatg ttaaaattca aaaaaatgag
    28321 tcagaaggga taaaagtatg aaaagagagg aatacaaaca aagattaaac gaactgcttg
    28381 aagaggacga aacattaact catggttcgc ctgatgaaat tctatacatg attgataata
    28441 tggtgatctt tggtggatat gaacttggta ataggtcagt tgatcataat attttagaat
    28501 ttgatgatgt atcatgggaa gaaattcttg attggggaat tttggcggta cctgaaacta
    28561 aaacctatat tagtgacact atggttcctt tttttgagga attggattat aaaagacttc
    28621 ccaaaaatga aaatcatata ctgggaggaa actaacaatg ttaagtgact tatttatttt
    28681 actgatctat ctaggtgtaa gtgaaattat tgcgtatttt ttgtttgtca ttgctaatta
    28741 tggtatttcc agtgcaatcg ttcaaatgtt tagattacca gtaagtgaac taaaaaaatg
    28801 atgagattat cagcatatct tttaatgttt ttgaatgtgg tcacgttaat ctttgtagta
    28861 atcggtgtga ctttcgattt aatggaatag tgggaaatct ataatggaaa aaagagacac
    28921 gaatgaacat attttttgaa tcaatattgt tgatggtttt tgcgatcagc gcttaccaga
    28981 aatctaaaaa agagtaatga ttgtttaatc atcactcttt ttttgtaggg aaattgaaat
    29041 atgtggagct tgtattggtt tagttaggat tgcttggcta taaggagcta gttctttatc
    29101 aaaaccatca aagtaggttt tcagtagcga tttaactgat cgctcatctt gtttgatttg
    29161 gataatttca gcatccaaat cggttgtgag atcaggagct tttgatgcga ttacctgatt
    29221 ttttttatct tccaactctc ttaggctgtt taacaagttg taaaaagaat cattttgatt
    29281 gttcattcca acgtgagttg caattttaac agaagtttcg tccgtattgt catcaaaaga
    29341 actcattgga atatcaagta gtaacgagga tgtttctttt actgactttt tctttacagc
    29401 attttttgtt tttccatctt ttttgctctt tactcgagat gtttttggag ctagtttagg
    29461 taaatcactt gttttatcag tcatttatta gcttttcctt tctattaatt tatgttatta
    29521 tattatcata ataacataaa aaagtacact ttttgggggc gtagttattg aaaaaatcta
    29581 agagaaagat tgccacattg cttatgttaa tggtgcaatt tacagggatt gcaatgagtg
    29641 taatcacaat aaatccgcaa gtgatacatg ctgatgggat agctacttat gatgcacaat
    29701 ttaactgtac tactgaaggt agtgggtcag gaacagatag cgatggaaac gatactggaa
    29761 gtggcgctgt aacaggtgat gtagccaaaa atattgaaga tatttacaaa tacatgaatg
    29821 gagaatatgg cttatcaggt gctagtattg caggtatttt aggtaactgg aaacaagaat
    29881 caggtattga tcctcgagca gtagaagggt attacggaga tggacagaga gaagttgact
    29941 tcatcaaaca atttactgat ggtctgatgg gtattggcta tggtcaatgg acagctgggc
    30001 gacatacagc attagttgat tggtctaaga aaaataatgg cggttcttgg tggactacag
    30061 aagcccaatt ggactttatg ttcaaaggtg atgggggatt cgtaggaata ttaaaagatt
    30121 atgctttaaa tgcttctgat gatgtcgtag aaaatgcagt aacttttcat aaaaactggg
    30181 aaatttcagc tgattccgaa tcagctgtaa tagctgtacg tggtgaaaat gcaaaagaaa
    30241 tctggaagta catgaaaagc aagggtatgg acggtgcaaa agatgaaagt aagatcaata
    30301 aaatatccgg aggttcaagt accggagatg gatcgaataa ttcatcaagt tcagcatcag
    30361 gaacagtaga agatgcctgt gcaactacgg atgacgctaa ttctggttct ggatcaattg
    30421 ggaaagaagg aaaaattaac ggtaaaaaag gtcaagtaat agcgaatatg acttatgaag
    30481 aagctttgaa aaaatatggg aaagcgatca cgctaccaaa attcaaagaa ccagattggt
    30541 caacttcacc ttttaacgga atatatcaag gtcagtgtac agagttgacg tgggcttata
    30601 tgagccaatt atacgatggg aatcaaccga ctactggaaa tggtggttat gtatgggaaa
    30661 gctacaaatc ggcaggagca aaaataacga acaaaccaat cgtaggttat gggtttagtg
    30721 cttcggatgg atggttatgg gctgttggag atgcagggca tactggtgta gttatagcgg
    30781 tgtttgaaga tggatcattt ctatgtgcta actttaatgt tccaccaacc caagctccaa
    30841 caaggggagt ttcggtaacg ttgattgatg gagtggacgg atcagataat gtgcatttct
    30901 ttagcggtgt aggaaaagct aaatttgaaa tcgaaaaata aattggaatg aaggacgata
    30961 tagatgaata gtaaagtgaa agatacaatt atcatttctt tgctttctgg tatgttatta
    31021 gcactgcttg ttggaatcct attcttcaat aagcagaatc aggacataga agctgaaaaa
    31081 cacagtgtgc atattgaaga taagctgaca ttctttaaag aggttaaacc aaatgaggtg
    31141 gatggattac ggaaagattt agaaaatgat attcaagaaa tgttgaatgg atcagttcaa
    31201 aataagtttg cagatcaatc aggtgaagaa tttttgagaa aactatttat tgtaacaggt
    31261 aaaccaccaa ttactgataa gagtagtgag aaagaaaaaa tcgagtacta taagcctttt
    31321 acttttaagc taacaaactt tggaattaag acatctagta gtgggttgta tgaagcattg
    31381 accgattttg aagttcaata caatggaaaa aaagtgtatg ataccacatt aaccatcaca
    31441 ctaaataatg ataagaaagt gactgggggt gctttttatg caaaatcatg aaatcactac
    31501 tgctaaaaag gcgatcggaa tactttccat aattgtgatc ctttgttgtg tggtactatt
    31561 tcaaaagaaa aatgcttatg ataataatgt caaagaattg gaagacctta aaagtaaaca
    31621 aatcgaagta gtcaagaata accagaaagt gaaagctatg caggaagctc aatacaaaga
    31681 attgggaaat gctgaaatta aggaacaaag taggaaattc tttaatttat tttacaactg
    31741 gaattcatgg gaaaaatata cgaagaatat gaaggaaata caaaaagaat ttcctcagat
    31801 agtaaagtct agtcctgttg atatttctgg cacgatggta ggcactggta atagtccaat
    31861 atctagctat actgctgatt actatacaac aaatcaaaaa ggtaaaatgg ttgaattgat
    31921 tacacagaca agaaatactc caacaacagc aacaacaaca atttggcgtg gagtaacaag
    31981 cataggagaa aatcaattgt tttatttgga ggatttagat agctatgtaa gagcagagta
    32041 aaataaaaaa agagctaacg gtaccaacgt tagctctttg tgctgacgat gataacaaaa
    32101 cctaccgaaa aaggattgtt tcatcacaaa cacaaacaga aaagaattga taaaaaaccc
    32161 gaacctcaga atttttatta atcaattcat ttagattata ataaattgag ctgaacatta
    32221 caatacgtat atgttgtaaa acgtaaaaaa agtcaaaagt aaatactttc ttaaaaagtc
    32281 aaataaaact attaaagaca atatatatgg tttatttgac ataagaaaat ggtctagatg
    32341 ataattgtaa tatagaattg tagacttaaa taaagcataa aatacctttt atgataaggt
    32401 atagctctta attttaagca gttttagata taagattgaa agtaaagtgg gggagaataa
    32461 taatggcaga tcataaatta gaaaagttat tagaagaaac gtggaatcca aaagaattta
    32521 gtgaattttt tatggagaat tttgaaactg acttagcagt gattgttaaa gatgctctta
    32581 gagagcaagg gtatcctgaa actgcaaact atataaacat taattttact ttgtatactg
    32641 aaaataaagg aacttgggat ttttgggcta ctttagcaaa taaggagtta agtgataaat
    32701 cagacacagg aattcgtaac ttttttgaat caaacagaga tgattatatg tatgctaaca
    32761 atcaaaataa attgaacttt agagtagagt ttgatgaaac acctgaagaa tttatagaaa
    32821 gacaaccgcc taaagaaaat gtagcaaagg tattggaaga tcgttggaat tcagatgaaa
    32881 tagtttcaac aattagcgaa ctaggcggac aatatgaacc gctagtagaa gcggttagag
    32941 aagagctaag actgaataag tttcctgatg ttcaaaatat tgatgtttct caaattgaaa
    33001 taaacgtcaa gatcacgaat aaacttgatt atggtagttg ggcagatata gcattagaaa
    33061 aatacatcta ctcaacgtta aaagagttta tcgaaaatag aatggatatc atgtatctac
    33121 agcatccaca gtatttaaac tttggtgttg aaatagctac acctcttgaa gaatggaaaa
    33181 tggagcaagg gcttgactag cctttgctcc atttttttat tttacaaatg aatacagttc
    33241 gtcgttttta ccaagaatga ttgtttgccc aactttgaga ttgtcaggtg atccttcagc
    33301 ggtttgtaag gagaacgttc cattgaactt atcggatacc gttataggta gctttaattc
    33361 tgatccttcc tcatctttga gaattgctgt ttttcgttca tcaaaataaa cagtctttat
    33421 taaataattt ttcgtagtga tctcagcaga tttgtatttt tttgtagtag taccagtttc
    33481 tttagaatta gaggtatttt ctacgactat tccatctttg gcatttgagc ttgtgtttat
    33541 ttgattgaat attttcagcg acaagataac gctaagaata gtcaatatag aaacaagtgt
    33601 aattaatact gataatttcc tttttttgtt cattttttct taacatcctt ttttgttttg
    33661 attagttaga tattccttat ctaacaagcc tttttaggct attaaatgaa gataaacccc
    33721 aaaaaatatt gcaattatga cagtgcaatg gtactattat tatacaataa attttgcgaa
    33781 aggggtaggt ctaaatcgca aacaaaaaga aattgaacct agacaaatac aacttgtata
    33841 ccgcaaggga agcaagtttg attttgggta aaggtagaaa ttatgttaca aacataatca
    33901 actattatcc aaatcgtttg cctaaaggtt cgttcaagaa agtgggtgac aaaacctata
    33961 ttataacgga agaaggaata gaagcattgc gatcacaaac agaaaataaa aacaaaaaga
    34021 aggaatgaat atgaagagaa attcgcttgg tatggtttta ttggcatcag ctgtacttgc
    34081 ggggttatca gtaccagtaa cctcacatgc tgatgaatgg actgcaaaca caccggatca
    34141 aatccaaatt acaacaggac aatcaagtta tgcgttcaaa aaaggagata ctatttggtc
    34201 aactgtagag aagcttaaaa gcttaggtta caagactact gttgctgagt ttgccgaatt
    34261 gaacaatgtt gatttggcaa aaggagaaca acggactatc ccgattggac gaactttcaa
    34321 catcagtaaa gatacattaa cagttaagga agcagatggc gaagtagtta gtcaaactgt
    34381 tcaaaaagat aaacctgatc ctattgaaaa tacggagcaa gagaacaaaa aatccgagac
    34441 tacaaaagaa tcggaaccaa cagcaaacac ttctgataag acagaaacaa gtgcttcaag
    34501 tgttgatgga gcaagttcat ctaatacaaa taattctaca agtgagaaag aagcatcaca
    34561 gggaacaagt gaaagtagca ctacggaagc tgatactgga acatcaacaa ataactcaca
    34621 atcaacatca aatgatgcaa atgaatcaac cagtacatct tctagtgatc aatcacaaac
    34681 aggttcgagt gatgaaactg acagttctac ttcaccaagt aaacccgaac aaccaactaa
    34741 tgttgtgcta ggcgttccat ttattagtca aggtaacaca atgctttgcg aaggaacatc
    34801 tttgctagaa gcgttacatt ataaaggtgt tgcaacaaat caagatttaa tgacttttgt
    34861 taaatcaatg ccactatctc ctgacaataa tccaaataat ggtttttctg gtgaatggag
    34921 acacaatgtg gatggaactt atcaaggtat gaatccggct cctgtagttt catggggtgt
    34981 tgctaatggc ggaaatgttg tagatatttc tggttcatcg gtacaaggat taaaagatga
    35041 gattgtaaat ggaaatcctg tagtagcgtg ggtaacttat gcctttgaac ctgctcaaca
    35101 taaaccaatg ttttggggcg atgctatttg gaatcgacat gtagtagccg ttgatggatt
    35161 taaagacggt ttttaccatg ttgtcgatcc tgtgtttggg gcaagttgga ttaatgcagg
    35221 atcatttgaa acagcatata acgtatctca attagcggtt tcggtacgtt aataaaagat
    35281 atcgtttata tactaagtag ttatatagtc tttatttgtc cttgcttaac tacttagaaa
    35341 acttgttgct catggtgggt agaactaaaa ggggtgatgc tatgaatact agagcgttga
    35401 aacagcttgc tttcaacgaa aaaaatgatt aataggtgtg gtgattttaa cttaggaatg
    35461 ggcgcgaagg tcattaccac accgacatat cgtggctcat tttcttcgcc taactggtat
    35521 aaagaaggag taacgagaga tgaagcatga aaaaacgata attttaaaat caaaaataat
    35581 ttattgtttt gcgactttag taatgatgtt atctacgctt ataatgcctt tttcagcaat
    35641 aagagctgaa gcagatgtca caaaaccgac aagtgcaaaa tttatagaaa ctggcgtaaa
    35701 aacagacggt tatattaggg ttaatatgcc gaatcatcca aatgaatgga tgatttcaag
    35761 tcaatttaaa gattctcatg gaaatattgg atattgtatg gattcagaat tgccatcccc
    35821 aacaggaagt ggagccggaa gcttgaagta caagggagct ggcagtgatg agttctatcg
    35881 tatgtttaaa ggtggttttc catcaaaaac agcaaaggaa ctgggtgcag gaaatgatac
    35941 agaagcatgg tatgcaacac aattagtttc atgggtttta gccggaaact ttaaagtttc
    36001 acagattgta tggtcacatc caaaccatac cgccgcagaa acagctcgtg taaaaaaagc
    36061 atttgaaaaa atttatgact atgctaagaa tggaaaagat acgcccaaca ctgagttttc
    36121 tatcacagct tctaagacag ctgacgaagg aaaatatcac actttcacat ataaaacagc
    36181 atcaaataag actggtaatg cgaagctcac ttttacatca gcaaaaccag ctggtatgaa
    36241 gatttatgat gcagatggta aagaaattac taacaacact gtgaaattaa attcaagttt
    36301 tactattaaa gtacctgtga ctacaccaag tggtactttg tcatttaaag gtactgctaa
    36361 tgttagcaca accaatcctt ttacttttga tggacgaggt gtgtatcaag atgccgtagt
    36421 tatgatcact acttcagaaa caaaggactc taaatctttg agtgctaaat ggacaagagc
    36481 tgttggtcag gtaacagtta agaaagttga tcaagatgga aatgctttaa aaggagcgga
    36541 gtttactcta actgacaata acaatgctaa atctgttaag acaaccggag atgatgggtt
    36601 agctacgttt gatttgcaac aaggcttaac atacagctta gaagaaacaa aagttcctga
    36661 aggttatcaa ggttcattta aaaaggatgg tattactgtt aataccgatg gacaagaatt
    36721 tagttacact gctacgaaca caattaaaaa aggtaaagta aaagttaaaa aggttgacca
    36781 agatggaaaa ccattagcta atgctgaatt taccttgacg gatgattcat cccaaacaca
    36841 agcagaaaaa acagatgaaa acggacttgc aagcttcgat attgtagcaa acaagaccta
    36901 cagccttgaa gagaccggag taccaactgg ttatacaggc gtattcaagc aagaaggaat
    36961 taccgttaaa gatgatggac aaacttttga atatacagct acaaacactt tgaaaaaagg
    37021 tttagtaaaa gttcataaag tcgatcaaga tggaaaacct ttggcaaatg cagagttcac
    37081 tttaaccgat gaaaaaggtg caacacaagt agaaaaaaca gatgaaaatg gacttgcaag
    37141 cttcaatatt gtagcaaaca agacgtatag cctagaagaa accggagtac cagtaggtta
    37201 cacaggtgca tttaagcaag agggtatcac cgttaaagac gatgggcaaa cttttgaata
    37261 tacagctaca aataatttga aaaaaggtgt agtaaaagtt cataaagtcg atcaagatgg
    37321 aaaaccttta gcagatgcag aattcacctt aacggatgaa aaaggtgtaa aagaagttaa
    37381 aaagacggat gcaactggtc tggctacgtt caacattatt gcgaacaaaa cttataactt
    37441 agaagaaaca aaagtaccaa caggttacac aggcgcattc aaacaagaag gaattaccgt
    37501 caaagatgat ggacaagtct ttgaatatac tgctaaaaac actttgaaaa aaggtgtgat
    37561 caaagttcat aaagtggatg atcaagggaa agcattagca aacgcagaat tcactttgac
    37621 agacgacaaa ggtgtaaaag ttgtacaaaa aacggacagc aatggcttgg caacatttaa
    37681 tattgttgcc aacaaaacgt acaaaatcga agaaacacaa aaccctaatg gttatacagg
    37741 gaaatggtct aaagaaggca tcaccgttgc tgacgatgga caaacctttg aatatacagt
    37801 aattaatcac cttgtacctg aaacagtaag aacaggcgga atcggtacat cagccttcct
    37861 tcttgtattt ctaattgcag gattgagtat ggtgctactg tattatcgag aacagcacaa
    37921 agcaaaataa aaaagtgtta atggaaaaga agtaaatatg aaaaaagaag gctgacgtat
    37981 gaaatcaaaa aaaatttttt caaatttaat gctatttggt actttgctag gagtgggatt
    38041 gggcgcaagc tcaatctctt ctagccttgg actagggggg caagtagcat atgcacaaca
    38101 aaactataat tatcaaggaa ctggaacgat tcatatccat aaagtaacaa atactaatgg
    38161 ggatgggaac cctgtctcag atattgataa caaagggaaa gaaatagctt taccaaatgg
    38221 tgtaaaacct cttgaaggcg ttgagttctc ttatgtgaaa cttactaaag atgaagctaa
    38281 atccttggga tggacggata gtgatactac aaaaggtcaa catattgatg atgctaaagc
    38341 acgattatta ctaaacaaaa aatacagtga agtaagtaga atgaagactg attcacaagg
    38401 gaatatagac atacctatac aagtaaatac agacgattta acagataact tgtatttgat
    38461 tgttgaaaca ggaacagtca aaggtgtagt tgaagaagcg tatcctatga ttgttaacgt
    38521 acctgaacta aacggatcag atgatcaata ctttaattat gatgtacatg tctatcctaa
    38581 aaactatttc caattaaact cagcagagct tgataaaaaa gacgaagatg gaaaggctct
    38641 tgccggaact gtttttgaat tgcgaaaaga tgatggatct gatcaggttg ttggaactta
    38701 taaaacagac agcacaggta aattaactgt agatggtttg gaattaggtg actattattt
    38761 cctagaaacc gatttgggaa cagatgctaa taaaacaacc tatttactag atggtaaaaa
    38821 attcaaattt agcgttaaac gtggggagac tgctaaagta gttcttgatg ttaccaacta
    38881 caaacgacct gtaattacta aggtaattga taaagaaacc gttgatactg gcgaagatgt
    38941 tcaatataca ttgaaaccat caattcctgg taatattggt aattacgaaa aatatgttgt
    39001 caaagatgtt ctaggtgcag agttaactta tgattcatca gaagtgatcg aaggaatgac
    39061 tttagatact gattacagta tcgaacgtga cggacaaacg acaaactatg tctttactga
    39121 atcaggtatg gataagcttc agaaacaatt tgatgcaggg gatcgcttct ttacaatttt
    39181 gatgaacgct catactaata gtaaagctaa aatggataca ccacttaaga atgatgtttc
    39241 aacgatctac aacaatggat atgaacccga agacgaaaca ccagttgatc acgcttatac
    39301 aatcattggt gatcaccaat tcattaaaac agatggagat acaagcaaag cattgagtgg
    39361 agcaaaattt gtagtgaaga acaaaattga tccgaataaa gttacttacc taaaacaaga
    39421 tccaacaacg aaatctgtta cttggattgc agataaatct aaagctactg aattcgtttc
    39481 cggatcagat ggtaagttcc aaattgaagg cttgcaatat acctatgatg tagattttga
    39541 taaacaaggt aatccaacaa tcacagataa aaaaatcaat gattatgctc ttgaagaagt
    39601 tgttgctcct tcaggatata caccaactag aaatgatgtt ccatttgtgg tatcgaatga
    39661 tgatgcttct agcgaagtaa cagacatcaa gaaccttaaa acacctgaaa cagttcgtac
    39721 aggtggaatt ggaacagtag tatttctagt ggttgcaggt gtggcaggaa cagggctata
    39781 tgttgtctac aaacgagaac aacacaaagc ttaatgaaat aaaattccct agagtgtttt
    39841 tggctctagg gtttcttttt aaaaagtgtg tgaaaagtag gtatgagcat gaaaaagaag
    39901 aatatcaagt tgatcatctt aaaaattggg atgttgctat gtttaggagt aggtctatat
    39961 cctgtaatta ctcaggctta tacctactac caaactcaac aaacactaaa ctcctataag
    40021 gaaaaatatg atgaagcttc tgaaaaggag aatgaagaag tcttcaagag atattcagaa
    40081 gttaatagga aacgaaaaga cacagaacaa acctcagtca cgattcctga agataataac
    40141 aatattccgt caaatggaaa tcttcagaag gataaattag gtgaattagt gggtgaaatt
    40201 cttataccaa agattaatgt ggatttgcca atttatgatg gtgtaaacga tttccaacta
    40261 tcagcaggag caggaatatt aaccggaact gattatccaa ctggtgggat tggaaaccat
    40321 tctgttatat cagctcattc aggcattcct aataaaaaat attttacaga tttagagttg
    40381 ctcaaacttg gtgataaatt ttttattgat atttcaggga aaaaacttgc ttatcaagta
    40441 gatcggataa aaacaattct accaaccgaa tttgaggatt tgcttcctgt ggcaggaaaa
    40501 gattatgtaa cacttatgac atgtacacct gttggagata attctcatag gttactcgtt
    40561 agaggggtta gcatacctta ccaaaaaagt attgttgaga aagaaaagaa gaaagaagat
    40621 ttaaaggtaa aggcgcagat gaactggtat cctatcgtag ttgtaggggt tttattaata
    40681 atagctgtga tagtcattat taaaaaaata gtagcaaaga gacgaggaat gaaaaatgaa
    40741 gaataaaata ctactttttt tgactcttat ttgcactttg gttttaacaa tttatcctgc
    40801 aaatcagctc tatcatcaat tcaaaacaaa agagaatgtg gcggaaatcc aagagaaaat
    40861 tgagaaaaaa caaactcctt caaaagagga agtggcaaaa aaagtcgaac aaataaaatc
    40921 gaacagcggt aaccaaggat tagacaatac tcagaaaaca gataaaggag tagacaatac
    40981 tcaggaaaca gacggtggat catttgatat tcgtgaagct gaaggaaagg tcaatccata
    41041 cacaggagat gttttttcaa aaaatcaaac gaaagatttt gaaccatcta cctattcagg
    41101 agcggttggt tatgtctata ttccaaaaat aaatgtgcta gagcttttgt ttctaggcgc
    41161 ttcacaggag catttatata gaggtgtggc acaaatgagt gctactagtc tacctttatc
    41221 aggtaaaggt catcaaacag taatagcagg tcatcgtggc ggttatacgt caccacaatt
    41281 tctttatgtt gatcaattag tagccggaga ccacatttat atagattatc ttggtgaaaa
    41341 agcagtttat acggttttcc gaacagacgt tattagaaat tacggtcaga attcaacact
    41401 gactgatatt cctgatttag atatgttaac actttatact tgtcatccat atcctcagaa
    41461 ctacgatcgt ttattagtta gaagtctaag agatgaaaaa gcaaaataca tcacgccaca
    41521 agtaacagtt ggcaataccg catacgctga tgccattgat agcgagtccg tttcagataa
    41581 gacagaaact gttacatctt ctaatgattt atttgaagat gttgatcgtg ccaacaagga
    41641 attagatgga aataatgatc ttaatattcc ggaacaaggt agcgtagaga tgcaagacag
    41701 aacgtttgct gaaaaggcaa gttcaatatt tacaggaatt accaataaac atcagaaaat
    41761 gcttttagtc atttggactg taataggagc attagcaagt ctaaaggttc ttattctttt
    41821 aatcaaagag tttcgaaaag cataatgaag attataaaaa ttgatagagt acagtaaaaa
    41881 gggattgata ctattattga tctctttttt attttgaata gagctagatt cacttttaaa
    41941 taaaatcaaa actgatctta attctaaaga aaaagtcaag aaaaaaatac aataaatttt
    42001 tttatcagaa tatttatcag aatattatta gaaaagataa actcaaaagc ttaatttaat
    42061 agggttattg gttgtattta gaaataatgt atggaaaagc tataaagcaa tagataagat
    42121 gtagaaaatc ttatttgtga atgaaaaaca agtcatcgta aaatattaat aggaggtgta
    42181 gtaatggtgg gagtatcaaa aacagcttta gactcattag ctaaactatt tagtagcagg
    42241 tttgatggtg cagaatttgt tgaatatcta agccaaaacg aaagtggaaa aactctgatc
    42301 gaaacaatga aaaaagacta tttgaatgaa aatgactttc cgacatggac gaatattgaa
    42361 gatgctacat gggatgtagt atgtacgata tattctcctt gggtaacgat tgctgatttt
    42421 gttttagata aaattggagg ggatacatac cctgatgcca aatctcttgc tacaagtgac
    42481 tttaaaacgt tctatggtta tctaataggt ctaataggca atggaataga tttgaaagtt
    42541 acgccaaaac aaacacctga agaaattgaa aaagaaaaag gagaggttca aatggcttat
    42601 gcaaatgaca gagaaaaagt aaaattattg gaaggtaaat tatctgaaga agaacttgga
    42661 aaaatggtgc aagaaatcaa tgaagacagc aactattttc ctgaattgaa ggtatatcaa
    42721 aatgacgaag atttctttaa tgaaatgttt agtaatagtc catgggaagc agtaagtaaa
    42781 acaaatttag gagaatggaa ctttcaagat gatctagttg catttagcaa tgatgggtat
    42841 ttacgatcat attcagaaag tgagtataga gctgaattaa aaacgtatga aacggatatt
    42901 ctgatgagct atttaaatct attagagagt ggtcagggag agttaagaga taaagttgaa
    42961 gaactattaa aagaagagct atagggatta tttcaacata aacacaagca gaaaactacc
    43021 aaacaaaacg taccatgcaa aaagtcggtt aaaccggctt tttgctctat tatgagagga
    43081 gatcaaacca tgagtgcaac aaccgatatg gctttagaag cagtaataaa agataataca
    43141 ggaaatgtcg aaaatgtaga agactttagc gatattgcaa tcaatctttc agcatggatt
    43201 tcagctgtag gagaagaaaa tatcgaattt gagatcaaga atatggatga cttaaaagca
    43261 aaatttcctt ttattgcatc gaaactaagt caagttgccg aggataaatc gtggaatgaa
    43321 ttagttagtt tttccgaaaa aatttatgat attacgcaag aagatgagtg tgtttcatgg
    43381 gaaatgaata atcaatacaa gaaaattgac gctttcgtca aatcacgtat agaaggaaca
    43441 gcttttgagt ttgaaggtat agataccgca gataattggt taaataaaga atcctttatt
    43501 aaaagtatag cggatgatct tgaaaaacaa tggtctaagg aagactatgc agagtaccta
    43561 aaaacagaaa gagatacaga agtgaaagaa aaactcactg aggtatgttt ggatgaacat
    43621 attgatccca atatcgtaaa acaagaagac tatgaagtgg ttattgataa agttcctact
    43681 gattttgagg aagttgtaaa gtatatagtt gaagaagcat tatcgcaata ctcttcagat
    43741 gaggaagtgg acgaagaaac tgttcgtacc agagtttggc aactggtttt tgatgacaaa
    43801 gattataaat atttgagtga tcaaaactcg gttaattgga gtgtacgatt attaattgat
    43861 caaaatgata ttgaatagag ctgaaaagct ctattcagat tgtagacaaa aaggagttgg
    43921 taattaagcc actccttttt gttagtcttt gcataatttt gaaataaaaa gccataggct
    43981 tttttgtttt ccatagccat gtggctagtt tttttaaatt catggcagca aacgtaagca
    44041 tcgtgtgcgt ggcgactttt tcaagacctc gatatttcgt ccaacgcata ccatgctttt
    44101 cttttgcatc tgcaaatatt cgttcaatgg tttgttttct ctttttgtaa atttctctat
    44161 tcaacgccgt atggcgttgg tgttcaagtt catccataag atcagcccaa atatgtcgag
    44221 tcactatttt ttgatgtttt gtactcaaag tacaattttc aagtaatgga caactcacac
    44281 agaattttgg atttgattta taggttcgat aaccatcacg agtgatagtt gaaaataaca
    44341 acggttgatt attgggacat aaatagcagt caaaataggc atcataaaca aagtcttttt
    44401 tacgaaacat tcctttcact ccatgaggtg cagtatatgg aagaatcggg tagagtttca
    44461 agtgtgacag aaaatgaaca aatttaggcg tcttatatcc tgcatcagca accacacgct
    44521 ttatatttgg aaaagctctt ttcgactgtt taaccagatc gattgcgact tggctgtcat
    44581 gcacatttcc tggtgtgatt aatgtagata agataaaacc gttatcttca caggaagtat
    44641 gtgcagaata agcaaattgt ttttctcgtt cacctttaac atagtaacca ctttctggat
    44701 ctgaatgact tattttttga agactcaact cactttctgt gtcccatgta aaaggacgtt
    44761 ttcctacagc aatacgttct ttatttattt catcttctaa ttcatcttga taagcttttg
    44821 cttgaccgta ggtcatctct ttcgtgaact tatgtttatt ggcatttgct ttgatatgag
    44881 tagaatcaat ataaaggttg tcttccgtaa cgaagccagc ttttacagct tgttctagaa
    44941 tatgagagaa aatatcttca aataaagttg tttctctaaa tcttcgaaca tagtttttac
    45001 caaaagtaga gaagtgtggg ataggatcct cgaatgaata acctaaaaac caacggtaag
    45061 caacatttgt ttcaacgtct ttaattgtct gtctcatcga acggatacca aataaatatt
    45121 ggataaatac taatttaatt aagaccactg gatcaatact gggacgacca taggttgaat
    45181 acgtagattc tacaatggga taaatgaaat caaactgaat cgcagcatca atttttcgaa
    45241 ctaagtgatc tttaggtaca agatcatcaa ttgttaacat agcaaattga tttctacctt
    45301 caatcgattg ttttctcatc attagaatca ctcccagaat agtttataca attagtataa
    45361 aacaaaaaac tgcaaacaac actccaaatt taggagtttg tctacagtct gaatagagct
    45421 gaaaagctct attttttttg ataaaaaaac tagctatttt attaaaaata atttggttca
    45481 aaatttattt ttttaacttg ataatttttt gccatccttt tttaaattgg ctatatatca
    45541 aggtttatag tgggaataaa taaaaataca tttgacaaaa tttacaatat agtttattat
    45601 atatttatct tattatgata acacaataaa aggaatggtg gtatgaaaaa gaagaagttt
    45661 agccgatcgt tgttaggaat tttggtttta gttgcggttg gtcttagtgg caagggttac
    45721 tttcagaata acagtactca ggaacatgat tttcagatca aaagtagtgt tcaggtaggt
    45781 aataaagcag acagtgaatc aaaagaattt ttagggatac gttatgatcc aaacaaatat
    45841 ccaaataata tcgtaaacat taatgatggg aaatatacac tgacaaaaga agaccatgaa
    45901 gcattatcag aaaaaggcag tcaaaccttt tgggtaaatt atgcagattt agatcaactt
    45961 ggaagagcag gagatgttga agcgttgatc acctatgatg ctgtaaattc tcactcatct
    46021 aaaaatgtgg aacgtccatc tttcaaaagt tctgtccata ttgcaggaga atacaaggat
    46081 ggagtatttg atcaagagag agatacttgg gtcggcaaag attcaaacaa tgaaatcact
    46141 cagctagaag gctataaagg ttatctctat aacaaaagtc attcattagc atggtcgcta
    46201 ggaggagata tggaggtaca caatttaaca cttggaacga gagcgcaaaa tgttgggaaa
    46261 aatgatcaga atggagggat ggcttactca gaaacgattg tacgtgatgc aatctataaa
    46321 aataagaatg aaaaagtatt gtataaagtt gatccaattt ataaagggaa tgaattagtt
    46381 cctcgtggtt ctcatgtcga agctttctca gttaacgata atggaaaaac ggtaaacctt
    46441 aatgtttatg tatttaacaa tcaaaaagga atcaacatca actatcaaaa tggtacttgg
    46501 gtaagagatt agaaattgag aaggtgaaga attggaaaca ataagcatca atggattaga
    46561 taaatcagtt gcaaatcgaa taaaaaaagg aaatggctac tatcttctga agaagggtaa
    46621 atattcagtt gctagagagt tagaaacaat gagtatcgtg tatgtagcta agaatcattt
    46681 tgacgtagaa caggctattt ttgatccaga ttctgaaata gggaaatgtg ttgaattatc
    46741 agagaaaact gttagtacaa aaggggcgat gatcattgag agagaaatgt tagacgaaat
    46801 cagagtaatc attcctaaag atacgcagat gtgtgaggac agcataagtg acgatgaacc
    46861 aaaatcagaa agaaaataag gagatattca gatgagcgaa aaatccattg aattaaagga
    46921 atataagcga agcgatggag aaaaatatta tcctgtagcc agtttttcta aatcagtata
    46981 tctggttgga cgtgcattgg tagttggtgc aattggcata tatattttgc attgggtaac
    47041 aaaggttgta caactgcttg tagatacctt agtaagtatt cttgtctcgt taaatgatgt
    47101 taacggaaca gatgatttag gattaaaaga gatgtttaaa gagttactca atgcgttttt
    47161 tcccaattat ttaagttatt catataatgt acaaagcttt tttgcaaagc tacacttggt
    47221 agacatctct attttagtat tagaagcagg aacggtattc tttgttggat atttggttac
    47281 tttgatgttc aggcatcact atggagaaca aagaccattt tttgatgata tggaaagtag
    47341 aaggttgaaa agagaagctc tagaagctat ggatgtgaag tatgatccaa acaagattat
    47401 aggagatgaa acgaacaaca aaaaaagaaa acgaaacaaa acgcaattag aatccgatgt
    47461 gctatcaacg cttaaaaata tcaaaatttc tgttcataca agacgtgatc taagtgtcaa
    47521 tgaagatatt cgagaatata tcgtaaaaat cccgcaacca aaagggaaga aagttagaga
    47581 aacagtttta cgacaaattc aagatttagg gtcgatcctg acaagagcaa caagcggacg
    47641 agccaaattt ggtgaaaaag aagatgaagt ttcaggcgat tatttcattt tccgagcatc
    47701 agtaaatatt gaaagagaac gtaaggaaag agtaagtacc aaaaacaaag aaacggaaag
    47761 agcaacatct tcaatcgcag tttcaaattc acaattttct tttccaatca atctctttgc
    47821 cgataattcc aaaaatatcc aaaaccaaac cgaaaaagct agaaaatatg ccgaaactca
    47881 aaagcaatct ttagaacttt atctatcttc tgctgacgta cctgcaagtc taacagatat
    47941 gcacgttggg aatacatcta ttgaatattt gtttacttta tctcgtacca gaaaaattca
    48001 actaagctca atgaaagaag aaattgaaac ttacttgcat atatcaggtg taatcgtgcg
    48061 gatcaacgca gggcgtattc aaattacttt accattagga gaaaatgatc gtattccaat
    48121 tgatgtacca tcaatgatca atagcgtgtt ttagaagaca tcacaaatag aaacagaaaa
    48181 tacaaactac cagttcacaa acagaaaagt accgagaaaa gtgaggaata gaaatggcta
    48241 tgaaattatt agagaaagtc aaaaagagag ccgaaaaagc tgaatctatt ttgctagata
    48301 aagaacctaa tataaagctt aatggaaaat tcagtgtcaa tatgttgaac acaaaggcta
    48361 aagacccaac agatgctatt tttggaatta agcctgatgg cacaattgct catttttgca
    48421 tcagcgaaat gccacacatg ttggttgcag gaacgacagg atcagggaaa tcagtattac
    48481 tgaatgaaat tcttgttaca gtaatgtgtc actcaacgcc tgatgaatta aaaataggaa
    48541 ttgtagaccc aaaaagggtt gaatttggaa gatacaaaaa attgccgttc atgttggctg
    48601 atccaattac agatatggat gaagcttatg atttttttga atatcttgtg atcttgatgc
    48661 atgaacgtta caaactgatg gaaaaagcag ggttacaaaa catcacgcta tataacgaat
    48721 atgccgaaaa aaatggatta gatagatttc cctatgtgat cttattagtt gatgaatatt
    48781 cacagttggt tggaacacat aaagaagtag aaggtttgat tgttcaatta ggacaaatgg
    48841 caagggcagc aggtattcat gttatcttag caacacaatc tcctcgatca acagttgtca
    48901 ctggtattat caaagccaat tttccgtcac gtgtttgcct tatggtagca agtgatttag
    48961 aaagtaggat tgtattagat gaaggcggtg gagaaagtct aagtccaaag ggagatatgc
    49021 ttattaagct tgttgatgga agtatggtta gagcgcaggg agcttacatc agtaacaaag
    49081 aaattgaaac aatcttcaat caccttagaa atactatgcc agaaccagaa tatgttgatt
    49141 accgagcaat cgtagctgaa actcgtggtg agggcggtga agacgaggat tttggtggaa
    49201 ttggtaaagc tcctgtaaga aaatctgcta aaagaaattt gaacacacct ttagcaaatc
    49261 catcatctaa agagccatca attgaaaaga agagtgcgtt tgctattaag cttgaaaaga
    49321 acaaagaaga aaaagtcgag cagggagcta aaacactaga aaaactgcaa gcagaaaaac
    49381 tagaaaaagt tttaagggca ttaggaaaag ctgatcctga accgcaaacc tcaaacaaca
    49441 cgaataataa acagccgatt gcatctaaaa ccaaacctaa agctaatgat ccaaaaggct
    49501 cagaaaacgt agtgatcaca actgataaat ctgaaaagaa acaaataaat ggaagtactt
    49561 cacctatcat gcaaaaaatg ttaaaagcaa aaaaacaggt gcataaatga tcatcgtcag
    49621 ttctttttta agatggctta ctccatataa atatacagat gaagtagttg tgaaacgagc
    49681 gaagaaaaga gataaaaaag cggtactaac tcctaaacag atgcttgatc atggcatttt
    49741 aggggtgaag gttttagaag tgaataaacc gtttgaatca aatagtgcaa gagaaaagtt
    49801 gcttggagct gaagccgatg tcgcatattt ttaccatgac aaaagcaagc atcaaatagc
    49861 tattaaattc aatccaaaat cggcatggcg gtatcaagca agagatgact tgaaacgtag
    49921 gaaacttaga ccaatgctct atccagaagg aaaagaagca tttgatagaa cccacatcgt
    49981 tcccattggt tttcatggta cagaatcaga taaccgccta ttagtaggtt ggaacagcaa
    50041 tcaaaataga gttcagatca ataaatttga gaagaaagta aggaaattca acgatcgaaa
    50101 aacgatccta tggtttgcag atatttgtaa acagccggat ggaacagcaa agtgggaagc
    50161 ggtgatattt gacgaaaatg gtaaaaaagt aatgtctgaa acatggcatg ataaagctaa
    50221 atttgtttgg cagtagcagt agaggtgaaa aagattgcta gaactttttg aagcaaatag
    50281 aacaggcttt aatcaaaagt tagaagtggg agaaactata gaatataaag ataaaaacta
    50341 tattattatc ggaatctata acacgagaat taacagaata ggtgatccac gtataaccag
    50401 tgacttggtt tgtcaatctg ttaactattc acctgatctg acaagcaggt ataagaaata
    50461 cgtgttatta gaatatagac acaagattac tgatgaaatt ggtgtaaaca acacacgaaa
    50521 catatttaag atcggaacag tgattcctta tagtaacaac gaagaaaaaa tattttatca
    50581 gatttacgga atagaaaagt ttgaatatga acatgtcgat ctgctagtga cgtaccaaat
    50641 gcgtttaata gaaccttggt cacaggcaga aattgataaa gcagtaaaat tgaaccgatt
    50701 atcaaaattt aacgtattag gaaatgcctt ttaaaaatcg caatacacaa acagaaaaat
    50761 accaaaaaat tagcaatgaa cgtaggtaag aacatgacaa taaataataa taaccctaaa
    50821 aaatataatg ctcttgtcca atgctatatc gaaaacaaaa ttacaatgca agaagtcgct
    50881 gatgaattag gcaaatcaaa gagcctagta tcacacgatt ttaagcacaa tgtttttccg
    50941 aacccgattg agctgaggaa aaaatttcaa aagagagttt atcaagaaat aggtaagtgg
    51001 attgatgaag ggaatgcaat tgacccttta attgaaaaat atcctttttt aaaaagcaaa
    51061 aataaaattc aaaggttaat tcgtcaaggc gatattcaag ctaaagaacc tgttttgaca
    51121 accaatgatt tggctaaaat aaatcttcat aaacaaatta ttgaagactt tgaaaaaaat
    51181 ctttcaacaa ctgaaatttt aaaaaaatac aatattccga gaggaaagct aacatcgact
    51241 ctttttagag aatatggaga atcggcagtt actaaagcaa aggaagtagc gaatgagaga
    51301 aaaaaacaaa aatagtagtc gatacctagt agcgactgta gtttttgttt gtgtattgat
    51361 tttcctgttt gattttttgc agattggaaa aagcataatt ggaaatacat tgagtgcaaa
    51421 ggaaatggtt gctttaggtg gtttgtatga aggacacttg ttcccgaata tattcatttc
    51481 tatcgtttct catattaact tgtaccattt aatttctaat ttattgttct tagtagttct
    51541 tgaagaagca ctgaatgatc agtatgagtg gttaacatta ttgatcgttt taatcatatc
    51601 aggatttcta ggaagcttag ttccttttct gctacacagc aacatcgtaa gtatggggct
    51661 tagtggaggt atttttggat ggattgcttt atttgcgatt gcatacatcc gttttgatag
    51721 caagaaacat ttattactaa gtgtttttgt attattgata atcggcattt tattggtatc
    51781 tacttttgaa tcaggcgagg caaatgtctt gtcacatgta atcggatttg tctgtggaat
    51841 gatcttggga tttattctaa agaacgtttc attgatcaaa caaaagaatt aaaagggagt
    51901 gctaaaaatg ccaaaaagaa aagaaaagaa aattccagtg tttttgtctg tctcagaaat
    51961 gttatcactt caaatactgt taagaaaaaa gattgaaaca atagacgaag aagttagaaa
    52021 taaaagggct atgtacgaac aacgtgaagc gctaagagaa cgaatccact attttgatga
    52081 tatacacact aaaatctatg aagcacgtac aaatcacgaa gacgaaagta aattaggatt
    52141 agctcaacaa cggatacttg aatggcttaa tacgaatact gatcaagaac cgttaagagt
    52201 gattagtaat ctttttgaat ttggaacgaa gcattggttt aatgctgtag agaaggatgc
    52261 tgacttacaa gaagcgtttg atacgctaag taaggatagc aaatccatgc gagatttatt
    52321 gattacttat ctaaatgagc atgagtttga acaaacgaaa accaaaaaga aaaataaaga
    52381 atagaaagtt gtgatgaatc atgctattaa ccctgttagg ttatttatgt ttaattcttg
    52441 ctttttttgg ggcaatcgct ataattattg gtcaatttca aacaaataat ccgattatgc
    52501 gtaacatagg cattggaatt gtgattgtaa cctttattgt gtttaatgcc actaagattt
    52561 ttggaagtaa cgataaacaa tttggtatat cgggagttaa taatagttat gctccagatt
    52621 ctttgggaag ggtagtaata gatggaacat ttgaaaatgt aacgagttat aaaaatgttt
    52681 cgctgacagt gaaggaagat ggcaagaaag catcagacga cgttctcgct actattaagc
    52741 cgtctcaatc acaatttgaa gtgttttata cagttaaaga gaatagccct gtacacaagc
    52801 tgacattggt ttttaacgtt gatggagaag aatactataa agatgtaaaa ataaataaaa
    52861 catcaacgaa aaagattatc gaatcagcga aaaaagaagt agctacagaa gaaagtacaa
    52921 ctgaatcaag ttctactgtc gaagcggaac taccagataa aattgatttt gatcctgaaa
    52981 aagtcaaatc gtttgaatct gtttctggtt ctatggctga tcggtttaac actgaagtct
    53041 tcaaagctat caaaactgtt aaattggata ctgaaaagca aatgattaat gttaccttat
    53101 tcaattcagt tgaatctatg gatcaggata aaaaagttac ggttgcaaga gaactaggaa
    53161 aaagagtaat tggtttgtat cgtggtactg ctaaaccaga ttcagacggt gcagatttgc
    53221 cagttcatgt agtttatgaa gacggtaaag tttttggaga ttctacagca aacgaagcat
    53281 taacgattaa attggctaaa tagccagaaa gttataataa acaaatggtg tcacaccaat
    53341 aatatatagg tgtgacacct tggagatgat agctgatggt agtaaataaa gaaaaagtta
    53401 gaaagtatgt gactttaaaa atagcttctg ttaaatattt agaaaagcga gctaaagaag
    53461 aagaaagagc aagaggtaag aaagtaacag tttcagagtt gttagaagaa ttgatcgaga
    53521 atgatcaaac aataagacgt gcatttacta ataggtctat cagataacaa tataattaat
    53581 aaaaaagaaa gagtgatttg gtcttagact aaatcactct ttttatataa agtttcttta
    53641 cactcattcc gaataatgtt aaaattcttt atatgatata tagttccttg tcagaggaag
    53701 agataaccag caatattgga gggataataa gtggaaaaca ataatgatat tagacatttt
    53761 agcattgggg aaagagttac aacagtaagt gaattatcaa aggattatag agataaagag
    53821 ggaacgatta tttctcttaa taagaatcga agaggcgttt ggcaatatgt tgttgattta
    53881 ggtgatggcg ttacagttga gtgtaaagat agtcaactga tcagaaataa tggcagtcat
    53941 tccattgaaa aagcaattct tgtataccca aaagtatcaa gtaagatcat gaatgactgg
    54001 ttgcttggaa aatttggtga gtacaataaa aaagaaatcg cagaagtgat cggcaaatct
    54061 tatccaacag tattagggta tctaaaaaag ataacgagta aagaagcaaa aattgaggat
    54121 gttttaacag taagagagct tcaatcatta atcgagtatc ggaataaaca aataacaaat
    54181 cctgagtacc ttagaaaaga agatggtcta ttgaaacaat tggaacaagt aaataaaatc
    54241 cggaagatgc aaaaggtagc tcctttaagc cttgatacct ttaaaaaaat ctatttgaaa
    54301 gagatcaaaa aagactaatg tggactgtaa ctttaccgtt ccaacctgta tctgcaccaa
    54361 gaccaagtgc aagaaggaat gctgctgatg aaactaatga cgtagcagag aaaaggattt
    54421 caacctacta tgataaaaag tatcttgatt atttgaaaaa aataaagagc tttatcgaag
    54481 atcaagattt attagatgat gaattttact cgattgtaaa tgatcctatg ggcgcaatta
    54541 tggaagtgga cttttactac cagataccaa aaagctataa aaaagtctat aacattatga
    54601 agacaacagc gccagattta gataatttag taaaaagtgc tatggatggt atttttaaca
    54661 tctcagctat acgagatagc catatcacag gcttaattgc ttttaaatat aatgtggcga
    54721 acgaaggaaa aacagttgtg aggatcaaac gatattcaga atttgagtgg gatacttctg
    54781 aaggattgaa cggagatata tgggaagcta catttgattt taaaccagta gcaacgccaa
    54841 gaccaaagtc taaatttaga ggaaatggga ttatcactta ctatccaaaa gagtacaacg
    54901 actatttgga aaatatcaag aatacgctta aagagaaaga tttggtaaat gatcaactga
    54961 aaaaggtaat gaatgctcct atgggggtta tagctcagat agattatttt taccaagtga
    55021 gaaaagataa gaaaaagctt gattcactga tgcgtacttc acagccggat attgataatt
    55081 tagtcaaagg aacattggac ggaattttta acaaggaaat aggcatcaaa gatagcaggg
    55141 ttgtagggtt aattgctttt aaatacaata cttttgaaaa gtcgaaaaca aacgtgagac
    55201 tacaggagat gggctgatga ataataatga aaaaattctt catgtacttg atagctttga
    55261 aataattcaa gaagaactaa aaaaatatcg ggatgttttg gaacaaagat atgattttgt
    55321 caatcagcaa aagagtaatc acatggattt cattctcaat atgaatgact tgaagaagaa
    55381 attagttgaa cgaaaagaac aagaaaagtt gatcaaagct tactttgaac tcggtgaaaa
    55441 agaagttaaa aatgcaatgg aactgaatga agagcgcagg gttttagatc agctgttaga
    55501 acaattattg gtgatgtttc aaaaaggacg aatagatgag gatttaattg aagaaggatt
    55561 gagaaaatat ccttcaaact caggtatcgg aatagtgctg aaagcgattg atgaagaaga
    55621 aattgaagct ttcataccgc cagaggattt tgaatcggca atggaatata taaaatatta
    55681 tagccaaggg atcactgctt ttagagagtt tgatcctgaa gatgttattc atgacttgaa
    55741 caacctaaaa gaatggtgtg aaagttatgg agtcgatgat tccggattgg actacttgat
    55801 cagtattatg gaaatcgaag aagagatgcc ggataagcct gatccaacag atattttaga
    55861 attgatccat gaagctagaa atccaattgc ctatatttct agaggatata ctgtattgga
    55921 atactataag ccttatatat cagcgatgaa ccatttaaga cgtgtattaa gggaaaaacg
    55981 tgaataccgc tcagtgttga atgcaacgaa tagattagaa aaagctgttt cagagctaga
    56041 tgcttattat cgtgaacatt acctgcaagc aggtggtatg ccaagaaata cgaaagcaaa
    56101 tatcagtagg tatataaaaa aagctgagta ggcggagtta gcccataaaa tggcaaaagg
    56161 taacctgaaa ggggcatttt gaatgaaaag ggatgttttt gaattgtacg tattagaacg
    56221 tctagaagac ggcatgatgg cacaagagat aattgatgaa attgatgata tgatcagcag
    56281 atttggtcaa ttggagttga atgatggaaa caaaaattcg gtagtgctaa aacaattttg
    56341 gttgaacaat ttttcgtata aggattttat caaagaaaag gcaaatacat tgtactgact
    56401 aaattcacga ggagcagtga tgagagctta ttgctaagag cagttaggct cttttttgtt
    56461 tgtgtaaaat tatttgtttt ataatacaca tttatacaca aaaaaatatt gacagatagt
    56521 acacaaatgt ggtaggatat atttgtaagc aagttagatt aacaagttaa aggaagagca
    56581 gttatgaaaa aagaagaact aatagatata tttcaaattg ttgaacgagc aaataatatg
    56641 ggaattatgt tttttgatcg tatttcttta aagatggatt taacagaatt aggcagaagt
    56701 cgcccatcct ctataggtgg gtgatgaatg ccgttcggta tgagggttac cgttggtaac
    56761 tcgaagaccg acaactaagg cgtattgcct ttttattttg tcccatgtta caatcagtct
    56821 atacaagaaa tgagttgata taacatggaa ttagatacaa ataatcattc agtatttctt
    56881 ctttattacc accttgtttt agtaacgaaa tatcgtagac aagtgattga tgatgaaata
    56941 tctgattatg ccaaaactac ttttgaaaga atttcagaat cgtatcacat tactttagtg
    57001 gagtggaatc atgataaaga ccacgttcat attatgttca aagctcaacc taaaacagaa
    57061 ttgacaaaat tcatcaatgc ctataaaagt gccagctcaa gattgataaa acgtgacttt
    57121 ccaagagtca aacaatttct ttggaa
//
LOCUS       DACCVU010000004        55347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCVU010000004 DACCVU010000000
VERSION     DACCVU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.004"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            205..573
                     /locus_tag="IVW29_000194"
     CDS             205..573
                     /locus_tag="IVW29_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7837705.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            753..1694
                     /locus_tag="IVW29_000195"
     CDS             753..1694
                     /locus_tag="IVW29_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7837706.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1763..2494
                     /locus_tag="IVW29_000196"
     CDS             1763..2494
                     /locus_tag="IVW29_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7837707.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2638..4818
                     /locus_tag="IVW29_000197"
     CDS             2638..4818
                     /locus_tag="IVW29_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7837708.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4838..5320
                     /locus_tag="IVW29_000198"
     CDS             4838..5320
                     /locus_tag="IVW29_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7837709.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5336..6097
                     /locus_tag="IVW29_000199"
     CDS             5336..6097
                     /locus_tag="IVW29_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7837710.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6228..7562
                     /locus_tag="IVW29_000200"
     CDS             6228..7562
                     /locus_tag="IVW29_000200"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7837711.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7869..8354
                     /locus_tag="IVW29_000201"
     CDS             7869..8354
                     /locus_tag="IVW29_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7837712.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8347..9030
                     /locus_tag="IVW29_000202"
     CDS             8347..9030
                     /locus_tag="IVW29_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7837713.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9156..11801
                     /gene="polA"
                     /locus_tag="IVW29_000203"
     CDS             9156..11801
                     /gene="polA"
                     /locus_tag="IVW29_000203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7837714.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11846..12682
                     /gene="mutM"
                     /locus_tag="IVW29_000204"
     CDS             11846..12682
                     /gene="mutM"
                     /locus_tag="IVW29_000204"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7837715.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12646..13275
                     /locus_tag="IVW29_000205"
     CDS             12646..13275
                     /locus_tag="IVW29_000205"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7837716.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13327..13659
                     /locus_tag="IVW29_000206"
     CDS             13327..13659
                     /locus_tag="IVW29_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7837717.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13971..14462
                     /gene="nrdR"
                     /locus_tag="IVW29_000207"
     CDS             13971..14462
                     /gene="nrdR"
                     /locus_tag="IVW29_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7837718.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14485..15858
                     /locus_tag="IVW29_000208"
     CDS             14485..15858
                     /locus_tag="IVW29_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7837719.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15858..16784
                     /gene="dnaI"
                     /locus_tag="IVW29_000209"
     CDS             15858..16784
                     /gene="dnaI"
                     /locus_tag="IVW29_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7837720.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16913..17353
                     /locus_tag="IVW29_000210"
     CDS             16913..17353
                     /locus_tag="IVW29_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7837721.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17497..18198
                     /locus_tag="IVW29_000211"
     CDS             17497..18198
                     /locus_tag="IVW29_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7837722.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18195..19316
                     /locus_tag="IVW29_000212"
     CDS             18195..19316
                     /locus_tag="IVW29_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7837723.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19331..20563
                     /gene="pepT"
                     /locus_tag="IVW29_000213"
     CDS             19331..20563
                     /gene="pepT"
                     /locus_tag="IVW29_000213"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7837724.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20695..21603
                     /locus_tag="IVW29_000214"
     CDS             20695..21603
                     /locus_tag="IVW29_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7837725.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21638..21910
                     /locus_tag="IVW29_000215"
     CDS             21638..21910
                     /locus_tag="IVW29_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7837726.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21921..22967
                     /locus_tag="IVW29_000216"
     CDS             21921..22967
                     /locus_tag="IVW29_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7837727.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23218..25827
                     /gene="clpB"
                     /locus_tag="IVW29_000217"
     CDS             23218..25827
                     /gene="clpB"
                     /locus_tag="IVW29_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7837728.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25978..26649)
                     /locus_tag="IVW29_000218"
     CDS             complement(25978..26649)
                     /locus_tag="IVW29_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837729.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26642..27136)
                     /locus_tag="IVW29_000219"
     CDS             complement(26642..27136)
                     /locus_tag="IVW29_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7837730.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27156..28376)
                     /locus_tag="IVW29_000220"
     CDS             complement(27156..28376)
                     /locus_tag="IVW29_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7837731.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28523..30358
                     /gene="lepA"
                     /locus_tag="IVW29_000221"
     CDS             28523..30358
                     /gene="lepA"
                     /locus_tag="IVW29_000221"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7837732.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30554..31465
                     /locus_tag="IVW29_000222"
     CDS             30554..31465
                     /locus_tag="IVW29_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7837733.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32461..32712
                     /locus_tag="IVW29_000223"
     CDS             32461..32712
                     /locus_tag="IVW29_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837734.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32795..33067
                     /locus_tag="IVW29_000224"
     CDS             32795..33067
                     /locus_tag="IVW29_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837735.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            33157..33396
                     /locus_tag="IVW29_000225"
     CDS             33157..33396
                     /locus_tag="IVW29_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837736.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33543..37826
                     /locus_tag="IVW29_000226"
     CDS             33543..37826
                     /locus_tag="IVW29_000226"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP7837737.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37925..38290
                     /locus_tag="IVW29_000227"
     CDS             37925..38290
                     /locus_tag="IVW29_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837738.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38292..38744
                     /locus_tag="IVW29_000228"
     CDS             38292..38744
                     /locus_tag="IVW29_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837739.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            39358..40668
                     /locus_tag="IVW29_000229"
     CDS             39358..40668
                     /locus_tag="IVW29_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7837740.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40691..41002
                     /locus_tag="IVW29_000230"
     CDS             40691..41002
                     /locus_tag="IVW29_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837741.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41363..42619
                     /locus_tag="IVW29_000231"
     CDS             41363..42619
                     /locus_tag="IVW29_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7837742.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42616..43161
                     /locus_tag="IVW29_000232"
     CDS             42616..43161
                     /locus_tag="IVW29_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837743.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            43162..43662
                     /locus_tag="IVW29_000233"
     CDS             43162..43662
                     /locus_tag="IVW29_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837744.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43743..44299
                     /locus_tag="IVW29_000234"
                     /pseudo
     CDS             43743..44299
                     /locus_tag="IVW29_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44319..44663
                     /locus_tag="IVW29_000235"
     CDS             44319..44663
                     /locus_tag="IVW29_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837745.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44682..45593
                     /locus_tag="IVW29_000236"
     CDS             44682..45593
                     /locus_tag="IVW29_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7837746.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45672..46664
                     /locus_tag="IVW29_000237"
     CDS             45672..46664
                     /locus_tag="IVW29_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7837747.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46661..46879
                     /locus_tag="IVW29_000238"
     CDS             46661..46879
                     /locus_tag="IVW29_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837748.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46892..47302
                     /locus_tag="IVW29_000239"
     CDS             46892..47302
                     /locus_tag="IVW29_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837749.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47354..49882
                     /locus_tag="IVW29_000240"
     CDS             47354..49882
                     /locus_tag="IVW29_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7837750.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49898..52108
                     /locus_tag="IVW29_000241"
     CDS             49898..52108
                     /locus_tag="IVW29_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837751.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            52116..53207
                     /locus_tag="IVW29_000242"
     CDS             52116..53207
                     /locus_tag="IVW29_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7837752.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53228..53770
                     /locus_tag="IVW29_000243"
     CDS             53228..53770
                     /locus_tag="IVW29_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837753.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            53992..54222
                     /locus_tag="IVW29_000244"
     CDS             53992..54222
                     /locus_tag="IVW29_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837754.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54236..54568
                     /locus_tag="IVW29_000245"
     CDS             54236..54568
                     /locus_tag="IVW29_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837755.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54613..55161
                     /locus_tag="IVW29_000246"
     CDS             54613..55161
                     /locus_tag="IVW29_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7837756.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            55168..>55347
                     /locus_tag="IVW29_000247"
     CDS             55168..>55347
                     /locus_tag="IVW29_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837757.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFL"
BASE COUNT        18814 a         9010 c        11908 g        15615 t
ORIGIN      
        1 ttttttattt attccatata aatacacatt atttattccg aaaagtgaaa gcgcaaataa
       61 caaaaacaac caacttattt attattaaag aaaagcgtat ttaagtagga cgattctgtt
      121 gagagctttc gtagattttc ccattttcct atggtaaact gacaaagttg agaatgttag
      181 aacagatcgt ttaggaggaa acagatgttt acgaatgaaa taaaaatcat gctttatgtg
      241 gacgatgtag aaaaaaatgc aacgttttgg agagcagttg gctttgcgga aaaagatcgt
      301 caagaaatgg atgggacgct tataattgaa gtaggagttg aggacagtca aacctcattt
      361 ctgttatatg atcgagcatt tgttgagtcc cattcacctg gcacagctac tagtgcccca
      421 tctttgatgt tttacagtaa agatatcttt ggactatata aaaaaatgca gacgcaatca
      481 gtcacattag gtgatatggt acagcttgga gaagaatatg tctttaactt tgctgatccc
      541 gatggcaact actttgcggt tacaggtaaa taatagaaat aaagactttg tttttctgtg
      601 cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa caaaaaactt
      661 tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg
      721 tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg ctttattaca
      781 attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat
      841 ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt actgtgtctg
      901 gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct cgatttgctg
      961 atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg tttgtaagat
     1021 accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac caagtgacag
     1081 gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag acagtggtga
     1141 ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct gtacgcccag
     1201 atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa gaatttatgg
     1261 aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt tctggttggc
     1321 ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct acttatggta
     1381 aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt gatgaacgga
     1441 tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa
     1501 caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag attcttgctt
     1561 ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg aaacatccgc
     1621 gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg cggtttacag
     1681 ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat gcgctatact
     1741 agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg atgtccatgg
     1801 caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc atcagcttgt
     1861 tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa
     1921 gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag aatatttttt
     1981 gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg gtggaaaaga
     2041 aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa ctgaaatctc
     2101 gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc gccccttgta
     2161 ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa agaaagactg
     2221 gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg aagggaaaaa
     2281 caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc atggagatat
     2341 gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg gggctgtatt
     2401 cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag atattgaata
     2461 tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga aggagctgtg
     2521 actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt agtttaaccc
     2581 aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg cattttaatg
     2641 acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc agactatcgt
     2701 cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt tccttttatc
     2761 gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg tgaaatcgaa
     2821 gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc
     2881 gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg
     2941 caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa agcaacgatt
     3001 gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac agatggagag
     3061 gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc tgccgaagaa
     3121 gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc aaaatttcgg
     3181 acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt caaggatgag
     3241 caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc cgtccataaa
     3301 atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat tctgaaagta
     3361 ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat agtcaaaaat
     3421 cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta taaacgcttt
     3481 attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc agatgagcaa
     3541 gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt
     3601 aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc agtcgtagat
     3661 gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc agcgcaagca
     3721 aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca agtagatatg
     3781 gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc tgagcagttg
     3841 aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc ttctgtttac
     3901 tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga aagaagtgct
     3961 gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa
     4021 gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc tgaacaattg
     4081 gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg
     4141 cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat tgtcatttat
     4201 cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc acgcttagga
     4261 ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa aaacattttg
     4321 gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt aacttcagtc
     4381 caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac acgattatcc
     4441 gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga tattttagct
     4501 gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt attacgtaca
     4561 gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac ggtccgtaat
     4621 gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact tgtccatata
     4681 tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt aggagatatc
     4741 gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt gacgatgctt
     4801 tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag aactgcagca
     4861 gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt
     4921 caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc atttagattt
     4981 taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca tcaaacatga
     5041 actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag atatggattt
     5101 caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga cgaagaaaca
     5161 gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc gcaagagaag
     5221 aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat ggattgagac
     5281 aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa ggcctttgga
     5341 aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt tagccgcacc
     5401 tgaagaggga aattttgagg aactgacggc acctacactt attttagctg gaaactgtct
     5461 tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag atcaattgtt
     5521 tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg aaaagcaagg
     5581 atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc taaaagaagt
     5641 ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt ctggagagaa
     5701 cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa aaatcttatt
     5761 gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag tatggcaaaa
     5821 tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc acttttctga
     5881 agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat atttccatgc
     5941 gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg gtccgaaagg
     6001 aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt acaccagaat
     6061 taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag cgaatcctcc
     6121 tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg agcgtgttga
     6181 atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg gaaaatcaaa
     6241 ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg gctcttgttt
     6301 tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc tttacacaaa
     6361 gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat gtaaaaccag
     6421 ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc cagcgagcaa
     6481 aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt atccaaaatt
     6541 atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga ctgctctctc
     6601 atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca ggacatggtg
     6661 atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat ttccttgcct
     6721 attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat tattatacaa
     6781 gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa aaagctattt
     6841 ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg atttattatt
     6901 atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca acttctggtt
     6961 ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta ccagcattcg
     7021 gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc gaaaaactgg
     7081 atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt cgattcagtg
     7141 aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct gcagagatca
     7201 aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt gcagttttcc
     7261 aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa gcacttgatt
     7321 tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa caaggaaacg
     7381 ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc aaagaaaata
     7441 atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt gctggagatg
     7501 ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa aatgtactgt
     7561 aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca aaaatcttgc
     7621 caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat ctgcggtaat
     7681 aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct tattatttga
     7741 tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat caagggagca
     7801 gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata aactggaggg
     7861 tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat gaaatcgagg
     7921 aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg ctgtatttgg
     7981 gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa aaaacagaat
     8041 atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt gcaatttcaa
     8101 agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt attgaaccgg
     8161 ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat aagatgaagg
     8221 atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg ttggatgctg
     8281 tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa agtgaaggtg
     8341 aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta tgcaaaagtt
     8401 tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt ggctctttgg
     8461 gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt tgtgggtctg
     8521 tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa gaatccgagc
     8581 ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt agcggttact
     8641 cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac tttgatgttc
     8701 atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt tggaagagca
     8761 ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt cattttgaga
     8821 agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc agggattaca
     8881 gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac agcacatagt
     8941 ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt gttcttggcg
     9001 tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag ttggggctat
     9061 accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat tctttgttag
     9121 aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa ttattactcg
     9181 ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct cttgaacgtt
     9241 tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg atgtttgaaa
     9301 atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca ggaaaaacga
     9361 cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca cctggcgaat
     9421 tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta cagtattacg
     9481 aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa gtagacaaag
     9541 atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta gctagtaaag
     9601 aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat acaccagaac
     9661 atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg aagggactag
     9721 ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa acagctatca
     9781 agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat gaaatgaagc
     9841 aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta tcgaaaaaat
     9901 tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg aagtatgaag
     9961 ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat cagttccttt
    10021 ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt gaagtcgttc
    10081 atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa atgatgggcg
    10141 acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag aaaatttatg
    10201 tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc actgatccga
    10261 ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac cgctatactg
    10321 gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc gatacgaatg
    10381 aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat atccaatcag
    10441 atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag gaactctttt
    10501 ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa ttgagccaag
    10561 aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca ttgtctatca
    10621 ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta caagaaatga
    10681 agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa gaagccggcg
    10741 aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa aaaatgggat
    10801 tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta ctggaacagc
    10861 ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt gcaaaaattc
    10921 aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa gtgcataccc
    10981 gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct aatttgcaga
    11041 acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt cctagagaaa
    11101 aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta cttgcgcata
    11161 tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc catgccagca
    11221 cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat atgcgtaggc
    11281 aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga ctttcacaaa
    11341 atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt gaaaaatacc
    11401 caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa ggctacgtcg
    11461 aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat tacaatatcc
    11521 gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct gctgatattt
    11581 tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt caagctacga
    11641 tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa cttgaatcat
    11701 tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta ccactgatca
    11761 ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt aagacagagc
    11821 aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa ccgttcgtaa
    11881 aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct tgtggcccaa
    11941 gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg aaaccatcca
    12001 atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg aactgatctc
    12061 acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa tcgataaaca
    12121 tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg atgtacgaaa
    12181 atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag gaatcatgaa
    12241 attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa atggactgaa
    12301 aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga ctggtctagg
    12361 taatatttat gtagatgaag cattatggga agcgaagatc catccagaac aaccggcgaa
    12421 cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg ttttggatcg
    12481 tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag gagaatcagg
    12541 cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta tacgttgtgg
    12601 aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc cgaattgtca
    12661 aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat agctaccggt
    12721 aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga tggcgatgtg
    12781 attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt atcagtgttt
    12841 ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg gatgattgtt
    12901 tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt tatccgaaaa
    12961 gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat tgtagatatc
    13021 ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt cgtttatact
    13081 acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct agaacaagcg
    13141 cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc ggatattttg
    13201 ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg gctttttgct
    13261 aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag aaagtgaggg
    13321 atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc aaaagaaaaa
    13381 atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca atcagaaaca
    13441 gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga ccagcaggca
    13501 gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac acctgatcgt
    13561 agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat cgaggaacag
    13621 cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa atcgagatgt
    13681 gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta gatgactaaa
    13741 tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga aaattgaaat
    13801 ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc ttatatccgt
    13861 gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac aactagttgt
    13921 aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa atgagatgtc
    13981 ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat gatggacgtg
    14041 cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca tttgaacgaa
    14101 ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag ttcaatcgcg
    14161 ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct atggaacaaa
    14221 tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa aacgaagtat
    14281 cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac gaaatcgctt
    14341 atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc ttaaaagagt
    14401 tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa gaaaacaatt
    14461 aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca accaaaaaat
    14521 atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc cttgctgtat
    14581 ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct gttatcagaa
    14641 atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct tagtagtcta
    14701 ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat cggcttactg
    14761 gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga gctgttagaa
    14821 ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct tctggaaaaa
    14881 gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag gcatacagca
    14941 gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa tgaagcagtg
    15001 tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga atctgaatca
    15061 gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga ttggctggca
    15121 aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat gtatcatcag
    15181 ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta tgactttagt
    15241 gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca gagacgtccg
    15301 gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc ctcgtcaaac
    15361 gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat tccaccgatg
    15421 aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca agaaaactgg
    15481 ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa tgttttgctg
    15541 aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt aaatacagtg
    15601 gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca gcacattcgg
    15661 caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta tcaaactggc
    15721 aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa agatgaaaaa
    15781 cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata cctggcaaaa
    15841 aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc atcagcaaac
    15901 gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat gcggatgttc
    15961 aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa aaaagttacg
    16021 ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat ccaacgatga
    16081 tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat gtcacttatg
    16141 taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc cgagtaaaag
    16201 caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa cgtacagatg
    16261 gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact gctgatccaa
    16321 aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa acatatttat
    16381 taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta gtccattttc
    16441 cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca gaaaagttag
    16501 atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat gcgatgagta
    16561 gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag gaacagttac
    16621 caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg tccgtcactc
    16681 aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata cgttatttga
    16741 caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt gacaagtcca
    16801 actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga cagaaatgac
    16861 cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt ttatgagggc
    16921 tattttttct agtatatctg tagttggtct attttttctt ttagcaggct gcggtagcgc
    16981 agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa aaacaagcag
    17041 tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatca ctcaagtaga
    17101 agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag aagatccagc
    17161 gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg ctgaaaaaat
    17221 aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat tagttgagat
    17281 gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta ttgatgtttc
    17341 attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt tatagaacga
    17401 aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc ttattttatt
    17461 tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga tttatctcag
    17521 cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga cattggatca
    17581 gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata tgcagttgct
    17641 ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa agatggatta
    17701 gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc acaagatggt
    17761 attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat cctagaagct
    17821 ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc aaacatcgga
    17881 gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc agaaaaaata
    17941 cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca aaaaccagtt
    18001 acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa aagtgagatc
    18061 ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga acagctcaaa
    18121 caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca aatgattgag
    18181 gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt gaaagctatt
    18241 gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga acattggata
    18301 agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc gaagaagcaa
    18361 tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt cctgtaaaac
    18421 ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa catgatattg
    18481 ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat gactggtttt
    18541 gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag attcattata
    18601 aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa gtattagctg
    18661 cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg attggacacg
    18721 gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg aagacagaac
    18781 aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa caagtgattc
    18841 aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc gctatagatg
    18901 aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa atttctatag
    18961 aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga atagtccagc
    19021 caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc ggtgaaaaat
    19081 tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat atctattatc
    19141 atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt cattatattg
    19201 aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa gaagaaaatt
    19261 gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt aattaaaagg
    19321 agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca aaacagaaac
    19381 acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg cttttgcgca
    19441 agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata atgaagataa
    19501 cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt ccattggatt
    19561 tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac aaatcattga
    19621 aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata cattgaatac
    19681 aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa cagatggcag
    19741 tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg caatggagat
    19801 cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg gtcctgatga
    19861 agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag attttgctta
    19921 tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg ctgctcaagc
    19981 aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata caatgatcaa
    20041 tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag ttccagaaaa
    20101 aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc ctgaagaagc
    20161 ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac gcaaagcaca
    20221 aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa tcaaagtaga
    20281 tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga gcatcgtgga
    20341 attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac cagttcgtgg
    20401 aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc tatttgccgg
    20461 aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg aaaaagcaac
    20521 agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa aaagaacagc
    20581 ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta aagggcagga
    20641 caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta aagaatggaa
    20701 aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa attagcacgc
    20761 tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact agctgaaact
    20821 ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga agctgatact
    20881 ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt ccaaaaaaca
    20941 ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct tgcacgtgtt
    21001 cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg caatgtacgg
    21061 aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac aatcggaatc
    21121 ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg ctcgtggatc
    21181 tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt ggatttagaa
    21241 acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat gtctaactcc
    21301 gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa atttcatgta
    21361 acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag aatcatgaga
    21421 ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat gaaaatccaa
    21481 gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga tcttccagtt
    21541 gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga agaaaaagct
    21601 tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt tacacaatcc
    21661 tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg ggtatgataa
    21721 acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat ttgtcagcct
    21781 gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata aaactcggaa
    21841 aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc tcatttattt
    21901 tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc attctgaatc
    21961 tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa tcatttgcag
    22021 atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct gttatattag
    22081 ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa cctttcgcgc
    22141 cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt taccgaatcg
    22201 tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa aaaggaatga
    22261 agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa ctggcagtga
    22321 tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct aatggtccat
    22381 caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt gcctttttat
    22441 tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg aataagttat
    22501 ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc tactctttaa
    22561 gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt tatctatgga
    22621 atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt gaaaaccatc
    22681 ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg ctttcagggg
    22741 ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta ttattcagac
    22801 cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc agttccttta
    22861 tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga gtgatcaaag
    22921 aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc ttttataaga
    22981 tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt acgtttgttt
    23041 ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata taagtcgaga
    23101 aaaaaagtca aaaaactatg tgtatatttt gaccaaaatt gaccaaagcg atataatagt
    23161 ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta tacctgtatg
    23221 aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca acagattgcg
    23281 gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt tttacaatct
    23341 aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc ttttgaacat
    23401 gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt ccaatatgga
    23461 caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat acgtgaaagc
    23521 ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa attaaaaaat
    23581 tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt gcgtaaaaac
    23641 atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga acaatacaaa
    23701 gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa aatggatcca
    23761 atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg aaaaacaaaa
    23821 aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt tgaagggctt
    23881 gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac gattttttca
    23941 ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga agaaagacta
    24001 aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt catcgatgaa
    24061 atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg aaatctgttg
    24121 aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt agatgagtac
    24181 cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt attagtcaaa
    24241 gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg ttttgagatc
    24301 caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt atctgaccgt
    24361 tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga agccagtgca
    24421 acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac tagacgtttg
    24481 atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc aagtaaaaaa
    24541 cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa tgcgatgaag
    24601 atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg tgctgaaatc
    24661 gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga acgcgctgct
    24721 gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt agaagctaaa
    24781 gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga gattgctcaa
    24841 gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga acgagaaaaa
    24901 ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga agcagttgat
    24961 gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa tcgtcctcta
    25021 ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc gaaagcgtta
    25081 gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag tgaatatatg
    25141 gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg ttacgaagaa
    25201 ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt gttggatgaa
    25261 atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga tgacggacgc
    25321 ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat catgaccagc
    25381 aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat tccagaagca
    25441 gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt cttgaaccga
    25501 atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg aatcgttgac
    25561 aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct gacaatcagt
    25621 gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg tgcaagacca
    25681 ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat cgttgcagga
    25741 catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact tcatttcaaa
    25801 acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga aaaaaggggt
    25861 ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag aagtagtttc
    25921 ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc tcttttatta
    25981 ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt ttttactttg
    26041 agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca agtcgcccat
    26101 cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag caaaacgttg
    26161 aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa acacgccgca
    26221 aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt ttgatatctc
    26281 agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga ccattggact
    26341 caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca tccctgtgcc
    26401 taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg ctggtatttt
    26461 aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc ctaataaaga
    26521 ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa attcataaaa
    26581 attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct ttttgatttc
    26641 tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc aactcaattt
    26701 ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag ttcaggtagt
    26761 gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg gttccgcctt
    26821 gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa gccacaccga
    26881 tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc attaaatcac
    26941 tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca taagcagttt
    27001 tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa gagagctctc
    27061 tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac attggtgcgg
    27121 caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct aaagtaagct
    27181 tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt ttctaaccgt
    27241 tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt caatgtttgg
    27301 atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg tccctgttca
    27361 tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc aatgatgaaa
    27421 tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt tacatcaacg
    27481 atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc aatcaaaaat
    27541 gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga aaaatgtgcg
    27601 gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat ttcttttacg
    27661 tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg gttgctatcg
    27721 tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc ttcccaatga
    27781 aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata aacggaaaat
    27841 acgtctggat gatcaactgg taatcgctca accataccac ttactgggat ttgtttaggt
    27901 agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca tcctttacgc
    27961 cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt cttgcgcata
    28021 ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata ttctggataa
    28081 gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt gacttcacaa
    28141 ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa cactttcgat
    28201 acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa cccgctttcc
    28261 tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa ttgatcgata
    28321 tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg atccattttt
    28381 tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa aacttctata
    28441 gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg aaagtgctga
    28501 ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc aagagcagat
    28561 tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg ccgatcgtat
    28621 tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt tagactcgat
    28681 ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac tcaattacac
    28741 agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc acgtcgactt
    28801 cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag tggtcgatgc
    28861 agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag acaatgattt
    28921 ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg aacgtgtccg
    28981 tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag ccagtgccaa
    29041 atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc ctgcaccaag
    29101 tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg atagttaccg
    29161 cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag acaaaatcaa
    29221 actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct cgccaaaagc
    29281 agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa gtatcaaaac
    29341 tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg ccgctgaagc
    29401 tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc caattgatac
    29461 ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg atgctgcttt
    29521 acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg gattcctcgg
    29581 acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc tagaattgat
    29641 cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga ctgctacagt
    29701 agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag aaccattcgt
    29761 caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac tttctcaacg
    29821 aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa atgtcgtcta
    29881 taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat caagcacgaa
    29941 aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt tagtgaagat
    30001 ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc accgagactt
    30061 tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc cacgccaaca
    30121 atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt cagacatcaa
    30181 agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc gtaaacgtaa
    30241 actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct ccgtcgaagt
    30301 tccgcaagaa gccttcatgg ccgttctgaa aatggatgag caagatagtg tgaaataact
    30361 tataatcaat cgaaaaacct attaaataaa cctttttgag tagatgcaac actgaggatt
    30421 atctgtataa aagattttaa actgtcattt ggttatcacg gcgatactaa aattaacaaa
    30481 attggaatat ttttcttgaa gatttttagc tgtcagtata tgatgaaagc atctcatagg
    30541 aggtgctttt ttcatggttg ataattcaag tggaagggtt cttaaatctc taagaaaaga
    30601 gaaaaaacta tctcaaaaaa agttagctga tttagcaggt atttcacaaa gtactttagt
    30661 taaatatgaa aaagggtcaa gaaaaatccc taaagacgta gataatactc taagcaagat
    30721 tctgaatata gaaactctaa taaaagatga ggaagataag attgagattc tcatcggtaa
    30781 actaattgca tacagagata tgaataagtt acttaacaaa gagttagctg ataatatagg
    30841 aattagtgaa gtattactca gttatgtgtt aaatagaaag agaaatccgt caaaagaaat
    30901 gcaaaaaaaa atcgatattt ttctattaag taatgaaaaa gaaattttga aggagattaa
    30961 tcgtgatagt gagatattta gtttatcaaa agacgataaa attgttatgg gaaaaagaat
    31021 acgagaagtc cgaaagaacc gggaagagac gcttgaaaaa tttggtaaaa actttaccat
    31081 ctatacaggc aaaaatgtta taagtagatg ggaaaaaggt attaatattc cagatattga
    31141 aaaactaatg aatattgcct atcttggtaa ggtcactgtt ccttacctta tgtacggaga
    31201 agattataag aatatacttc caaaagatga aagagttagc gattttaaaa agataaattc
    31261 ttttagtatg ggattaagga tgaggaaaat taggaaagat tattatctag aaagggagga
    31321 gtttggaaag cttttttctc cttcaatttc aaaatggtcc atagataggt atgagaatgg
    31381 tagagatata cccaatacta atcgaattat ccaatatgct tatataggaa atctttcttt
    31441 agaatttttg atttacggga tataaaaata gtttacgtcg atacttatta agttatcgac
    31501 gttttttatt atagaaaaaa tttataaaag gaggtttctt gatatgataa gaaacaaaaa
    31561 gggtttgttg aaagttgctt tactggcagc ttttttctaa gtaacttttt gacaagctca
    31621 atggcaacac aagcggaaga agtaatacaa gaactaactg ttatgaataa cagctagata
    31681 aaatgggcta tgtatgttat ccaacaattt agtaatcact agaagcctag cgaaagttcg
    31741 cttgataagt tatagatgag tattgatttg tagtgtttcc aaatcaaaag ggtacagagc
    31801 cacatgaaat atgttgtcct ctgcagcgtc aggatgggaa tccataccat ggagattcgg
    31861 agatctgtga gggaatactt acgtggttaa tatgtcagtt gtgttggacg tagtaatggt
    31921 catagtgggt agattgactg aattcttata tgagaaaatg aaaccaacaa gaagataggg
    31981 ttatttatac agaattgggg atcttttctg gaaaagtaat cggataatgc gagattcgta
    32041 cggtcttttt taaatttctc taaaaaaaac ggcatcgaat gggttgggtg attgataagc
    32101 aatgaatcat agactgatat agaataaaaa atgattctat ttatcttttt acatctatcg
    32161 ttttttcaag tagccgcaaa ttatatcagc attcaacaat ttttgtatac aagttctagt
    32221 aaaaggatct gaagaaaaag ttggttgttg tgaagataaa aaaagaatca ggttttactg
    32281 agtttttctt caaaatttgt gttattaggt taggacaaag ataagaagaa gtatgaagat
    32341 ttaaaaaagg aatgaggaaa agatagtaat ttccgattat tggagttttt ttatttataa
    32401 agaagaagcg aataagttgt gattcctaga tttcgcgcgt gattaattaa tgagcaaaaa
    32461 atgcggaaag ttttagtgtt taatacacga ggacaaagtt tcttaggtat tgaaggcaca
    32521 cgtgtaacgg ttgatgggtg gtgtgattgt ttcgaggttg gaaaacatgt tgaacttatg
    32581 gcatttactg agctaaaaga taagaacaac gtggaatttt tcgagagtga tattggttgg
    32641 gacgatcatg aagaagttta tggaaaattt attttgcgga agggaaattc ttatacgaat
    32701 ggggaagcat aagtagcgat ttgttagaga tcattgatag gattgaaatt gtcgggaata
    32761 tttatgagaa tctagagtta tttgaggtga tagaatgagt gatggttttg aacaagtatc
    32821 aataattaaa aatcaagttc gggagattct tagaaaaaaa ggctatttgg tagatagtta
    32881 ttttgaagga gattacgaaa catgggtagg tgtctatgct cgtccagaga ataaacctac
    32941 ctatttagat cctacgacgt cagaagatgc ctatttacaa aataggtatc gagtagatgg
    33001 ttttaagcaa gactttgcgg agtggtttga atgggaaatt gaaaacggag aagtaaaaga
    33061 agaataagag tacttgttgt atcaaaataa gccagtagaa caaccaaacc agtagctagt
    33121 aatgacaata atactgagga atcggatcat tatggtatga ttgatatttc tcttccagaa
    33181 gatcttaagc cagatcctgt aacaattggc aatagtgtca tgatttttga ttcattagat
    33241 gaagctatgt catatggttg gaaagaaatt aaggatgaag gtagtaattg gtatggcatg
    33301 agatttggtg atggtgaagt tgccaaaaaa gctggtattc caattgggaa atggacagtt
    33361 gatttccacg aagcaggttc tttttttgtt ctgtaattat agtgggaggg taaaatcatt
    33421 gatgaaatgt gttcagataa gagcgccaga taaaaataga agagacagtt cacgtcgtta
    33481 tcacttttgg taacggcgtt ttgttttatt atataaaatt ctcaggaaaa ggaggaattc
    33541 ttgtgtatgc aaaaacgaaa atgaattggt tgaaagcact cgcattggtg gctttttctc
    33601 aattctgttt cttggtggga actgctcaag cacatgcaga agaaacgacg gctatacaac
    33661 ctacgatgga agccattgcc caagtcgctt ctgaagtgac agaagtccaa ccagcagaag
    33721 ccggggtccc agctggtgaa gccactgttg aacaggcccc tgtgacagaa gcgccaacgg
    33781 tcaattccaa aacgactctc gatcagacga ttggggaaac acaacaccca gggaatagtc
    33841 ctggtacttc acaaccagtt gaagaaactg cctcaattac aacccctgct tcagaaacgt
    33901 ccactctaga aggagaaaaa gctgccgaag caacgccttc tgtgcagcca gagtcttctg
    33961 agaaacaagc ggaagaaaag aaacgaatag aagaaacagt ggaacaacca gcggctactg
    34021 ctgacaaaga aggaaaaaat gaggaaaacg ttgaaaaaac aatctcttca accgcagcta
    34081 gtcaaaatca accaacgaaa ccaaacaaag aatctacggc tagtattgaa aatcaaaaga
    34141 aaaaagagca gaacattcca aaaacaggta cacctacact gttagtaaca gtaccaacca
    34201 atcgttcggt tcgtgttcgc cgtgcaacca atgcagctgt tttaggtggt cggaaaattg
    34261 attggggcga aaatattggt gttacttcta gtccgaacaa tctaaacttc actttaaatt
    34321 atatggttcg ttttgagatt aatggacaag tggcgttttg tattgagcct ggtgtctcag
    34381 ccgcagaagg tcaagtttat acagctactg ctttgaaaaa ctatttgaaa aattcaaatg
    34441 tacggaaaaa aacaagcttg atctcttatt taggatatgt cacaaataaa gacaaatccg
    34501 atgaacaata cattgcggca caatttatga tttgggaagc tttgggcaca aaaatcccaa
    34561 tcaaaaatat ccgtgttgat tatgatcgtc gaaaagcaga aattcaacgt ttgattaata
    34621 actttggtga aaaagcaact tttaataatc aaacacatac ggtaaaaatt ggcgaaacgt
    34681 tgaaagtaaa gaataaaaat gattttttga aatatgtgaa agacattcaa aaaccaaaag
    34741 gtgtcaatgt atctattcac ggcaatgaag tatggattac agtcacaaaa gaggcaccgg
    34801 ataaagcaaa aattcaattg aataaaatta aagttcaagg tgcgccatta gcctatcgtc
    34861 gtccaggagc tcaaacaatt ggtgtgttac atccaacaga acctaatgcg tctgttatta
    34921 atttacaaat tctaaaacaa ggaaacattc gtgtgtttaa agaagatgcg gaaactggtg
    34981 tgaaagcaca aggtgcagct tctttagaaa atgcggtcta tggtttgtat caagcagatg
    35041 ggaaaaagat taaagaaatt accttgaaaa aaattaacgg caaagtacag gcagaaatta
    35101 aagatcttga tcctggcaaa tatgttttaa aagaaatcaa agcgccaaaa ggttatgtgc
    35161 tttctgatga agcaattgaa attgttgttg aacctggaaa aactgtcaat gcgaccgcaa
    35221 aagatcaagt gatcaaagga accattgatt tagtgaaagt tgctaataaa gatttagtcg
    35281 attcaacgaa tccagataac aaaccaaaat tagctggcat tgaaatcact ttgacgtcaa
    35341 aaacaactgg caaagtggtg aaaaccgttg tgacagacaa tgatggttat gctacttttg
    35401 gtaaaaatac cgttgtcttt gatacgtaca ttctttctga aaccaaagga aaagaagggt
    35461 ataaactatt tgaaccactt gaagtcacaa tttctgaaaa tgggcaaact ttccattatg
    35521 ttttagaaga taaagtaatt gaacaacgtc ttaaagtggt gaaagtggat caagaaacag
    35581 gaaaagtgat tccacttgct aatacagaat tcaagatttt tgatacttta gccaatcgtt
    35641 atgtcacaat ggagattcca aatgatacag aaacaacgga tatctttaaa accaatgaaa
    35701 aaggcttttt cctaactaac ggtacactta cttatggaaa agatcgctat cgtttggaag
    35761 aaatcaaagc ccccgtaaat tacgttttga atcaaaaccc attagtgttc tcgattacca
    35821 atgatacaga accgatcaaa gtgatccatt ttgcgaaccg tcaagcacgg aaaagtgtaa
    35881 aattattcaa gtatgaagaa tggaacaaga agaaaacgcc gctcgcaggt gtgaacttta
    35941 ccttgtataa cggtaaaaat caagcaattg gtgaatatac cactgatcaa aacggggaaa
    36001 ttttagtgaa aggtctagtt gccgatgact attacttctt ggaaaatgcc ccattagaaa
    36061 gtttccaacc aaatacagat aaacaagcat tcacggttgc attcaaacaa aatgcgccag
    36121 atgatgggga aacgttagtg gtttctgtac gtaattactt gattccacca aaactagaaa
    36181 cacaagcgtc aaataaagcc gatggtgaaa aagtggttga tccattagaa acgatcgtca
    36241 ttaaagacag tgtttcttat accaacttgt ttgtcggaaa agaatacacc atttcaggag
    36301 tattgatgaa caaggcaacg aatgcgccaa ttttacaaga tggcaaagaa atcacaagta
    36361 ccttggcctt tattgcagac aaagtcaatg gcacaaaaga ccttgaattt gtggttaatg
    36421 ctagtgttct gaaaggtcaa acgatcgttg tttttgaaaa acttttccgt gatggcattg
    36481 aagttgcagc acatgcggat atcaacgatg aagcccaaag tgtgaaagtg aataatccaa
    36541 ccgttcatac cacggctgtc tttgaagacg gattgacagt cgcagaccca acaggattgt
    36601 ttaccttaaa cgataccgtt gaaattaccg agattcttcc aggaaaagca tacttggtca
    36661 aagggatctt aatggataaa gcaaccggtg aacccttgct tgtgcatgga aatacggtag
    36721 aaacgagtgt tggctttatt gccaaagcag aaaatgaaac ggtgatcgta ccatttacct
    36781 ttgatttgac aggtataaat ggtcgtgaaa tcgtcgtgtt tgaaagtctt tttagaaatg
    36841 atgaagaact agcaaaccat aaagacatca acgacaaagg gcaaacggta cgagtaacta
    36901 acccaagtgt acgtacaacg gctacacata aacccactgg cttgaaagag gttaatccat
    36961 taacgaaagt caccattaca gacgctgtga aataccacga tttggtcgtt ggaaaaacat
    37021 acacagtcaa cggtgttcta atggacaaag taacggaaaa accagtgcta gtagatggaa
    37081 aagaagtcac aagtacctta acgtttgtgg cagaaacaac ggatggtaca gtcaatctag
    37141 actttattct agacgcacgt gcgttgcgag gcaaagaagt cgtggtcttt gaagatctgt
    37201 ttagagaagg cgtattattg gcttctcacg cagatattac cgacaatgac caaaccatca
    37261 aaatcagtaa tccaaccatt acgacgaaag cgacgaataa aaatggcgga aaagagatct
    37321 tggccttgcc aaatcaaact gtgcttgaat gggtcaaagt agacggtctt gcgattggtc
    37381 aaatctatcg cttaattgtt caaggctact taacaccaga cggcacacca tttgaaggca
    37441 ctcatgcgga aaaagtcttt gcggccgata aagaaatgat ggaattctta tttgaattct
    37501 tagtggacgg tcgcaatctt gcaggaaaag gcttgagttt tgcggaatgg ctccaagcga
    37561 aaacggaaga tgagaaagaa acgtttgaag aagtggcgaa acacaatgta gatctaacaa
    37621 acaaagatca aacggttcaa attgtggaag cgccaaaacc accgcaacca gtgaaaccag
    37681 aacaacgggt taaaccagct actgttgtac caatgttgcc aaaaacaggt gaacaaacaa
    37741 gtagtatact tatggttctt ggattagtag tattagtcat tgctttaggt ataacacttt
    37801 ataaagtacg taaagccaaa gattagtcat gaggaacaag cggttaggat ttttaaccgc
    37861 ttgtttgatt gtaatgtaga aatatttaga atttattttg gtttttaaga ggaggaaaaa
    37921 ctttatggca gaaaaagcat tgcgtatgaa gaaagagttt ttaggtcaag tatttttatt
    37981 gggttcacgg gaacctaatc caccaaagga aattaatggt gtgtctgggc gatatgttca
    38041 attacaagca gagtgttcgc gagaagaaca gcctgctttt tttgtttatc tcacagctga
    38101 agaggataaa agattacgat taccagaaaa agaggtgcgt atttcatttg aagaagctta
    38161 ttttgttttt cgtaaagaac gagtgaatag tcgtgaaaca aatgatatgg gacaaacaga
    38221 gcgtcgaaca actggctatc aagatgttgg tgtcttttta actggtgtaa aaattgggga
    38281 ggttttgtaa gatgggatta aaatttccaa ataattatga cattacagaa gcattagatg
    38341 aaacttgttt aggagccgta cgctttggcg cattagaaga gtctgatcgt gtatttgata
    38401 atgaaacagg cgattatacg ggagaaattc gatcacagcg agcacaatta gtttttggaa
    38461 atccagatga accaaagatt gcacgacgag ttaaatttcc agcagatgta gatttgactg
    38521 gttttaaacg acgtgatcct gtgaaattaa ttgatccaga atatcatgtg gtatatattc
    38581 cagcacaaga tggcaatcgt gaatattcag atttagtgat ttatgcgaaa ggattagaga
    38641 aagtagaagc tccaacaaat aatcctaata tgaaacaatc acaagaaaaa ggtttagatc
    38701 aaaaacaacc aagtgttgat tctaaggaaa aagagaaaaa ataaggtttt tttgaaagaa
    38761 taggaaggag tcttcctatt ctttttatct aagatagaag gaaggggaga ttttgaattc
    38821 agataaaacc aatcaaatac tttgagagat tgtgcttgtt atcaaaatat ccaacaattg
    38881 attgagtatt ttggagacaa tccaacgatt tttgtacaaa caaatgcata tatttgtgcg
    38941 gttattgggc agtgtatgtt tttacgtgat cttgttcgtt tttcattacc gtaccagtgg
    39001 aaacaagatc atcaggaagt gatttggatt cgtatagtac gaatatgtaa tatgtgtgca
    39061 cacccttata aaaatcgatt aaatcttttt cgattatgtt tttttactaa atgtgatatt
    39121 cctgtattag ttgctttttg tcaaaagttt ttagcacagg aaaagtaaaa acagaaagga
    39181 ggaatgtgac aattgaaagc tcattttttc aagtatcgtg gtcggcgaat tcgctatttc
    39241 aatcgttatt tatggttttg ctattatggt ttctttttgt ttccattttt gattctgtta
    39301 ggttggttta gttggaaagt attttatcca cgtttaagta cgttttcgaa ttggcaaatg
    39361 tatcttattc ctggaataag tattgtgttg tatttgttgc tggttagtgg gttaatttta
    39421 gggttgatgc attggtcgcg actaaaagaa ggatattttg cctctgttta ttggcgtcag
    39481 ttacttgttc gaatgttaat tgacaatcgg tttgtttata caaagaaaca agttagtgag
    39541 aaacaaacaa gggaaaaaat gagattacca aaagtttatt tttatcaaaa gaaacaagaa
    39601 ttagtggttt cttttccttt agatggtggg cgttttcacg aacgattttt aaaaatgggt
    39661 catttgttat ctgaagtatt tttgcgtgat ttaattcgtg aagaacgaga aaaagcccga
    39721 atcaattatg tattcctttc agattctggt cggattccta ttgatcaatg tatagcagaa
    39781 aatggacgta ttcatttaat gaaaactttg gattgggtat atgatcaatc accaaatatg
    39841 ttgattgctg gtgcaatcgg tggtgggaaa acctatttat tgtattcttt aattcaagct
    39901 tgtttaagtg ttgggacagt agatatttgc gatggaaaag cggcagattt agcagcatta
    39961 ggtgacgtcg atgtattcaa aggacatgtg ttttacaaaa taaatgaaga aatgattatt
    40021 tgtttacgaa atgcattaaa agaaatgaat caacggtatg cctatatgaa aaccatgaaa
    40081 gatcctaaat atgaaccagg gaaaaattat gcttattatg ggttaccacc gcattttatc
    40141 ttttttgatg aatgggcagc tttttatggt tcgttggata tggaagcgag aaatagagtc
    40201 gataaattga ttcagccatt agtattaaag ggtcgtcaag cagggatgta cattatttta
    40261 gcaatgcaac gaccagatgc agaatatttt ccaagtggtg ttcgggataa tttaacattc
    40321 cgtatttctg ttggtcgatt gtctcccatt ggttatttaa tgacttttgg agaagatttt
    40381 aaaaataagc cattttacaa caaagatcaa cctggtcgtg gatatgcttt tccaggattt
    40441 gatgtacctg tagagttttt tgcgccattt gtttacaaag attatcactt tatcaaagaa
    40501 tttgagaaat ttactgaaat gattgtctat gattttacag taaaagagga tagtcaagat
    40561 caaacgatgg gagatgataa tcagttgtta caagaacaac tagagcgcca aatgtattta
    40621 gcaaaagatc caaaaggaac aagtttttct gatgcgcata ttttgtaatc aactaaggag
    40681 gtgaagttaa atggcagaga gttgttatta tagagtagct acagcaatac gaatgattaa
    40741 tccttctctt tcttccagaa cgttttatga ttggttgaat cgtattgaac aagtaactga
    40801 ttaccgcttt ttgcgcaaag aacgtgtttt tacaggaaaa gttattaatc aagtattgct
    40861 aacgaaaaaa gatatagaac gattgactcg attgtatcat tatcgagtag atttagaaga
    40921 ggatttaact ttgtcaattt atcgcgtttt ttctcctgaa aaatatagtg aaataacaaa
    40981 attagatcat ttaattttgt agttattatc tatcgggagg gctctagacg tagtctggta
    41041 gccgcactct gcctggcggt gcgcattaag gctgcgaagc atgggcctat aatgcgcact
    41101 cgctagcagc gtgtgcagct cacaaagagg tcgtgcgctt gcgcacggcc tctttgtgta
    41161 aagccccccg atatataata tggggggcag aaataacata acacatagaa aaaagtttta
    41221 taaacccttt tgtatcaagt gattatctaa ggtcagcagt tgtactactt ttcccaaaaa
    41281 acgaggggga ctagttgata tatcggtgtt tctaaagggt taaaaaaaat cgattttgtt
    41341 attttataaa aggagcgaga aaatgacacc gattgagcta aaaagctaca gaaagaagtt
    41401 aagtgcaatt cataatataa gtggagataa aaagccgcta tcacaagcaa gatttgcgca
    41461 attgatgggc tgctcaaaag attacattag aaagatggaa caagggaaaa gaaaaatccc
    41521 agaggatttt gaacaacgaa tcaaggaacg ctttcgattg tatatgaaaa gcggaataga
    41581 tcttcgagca atgattgatt atttgcgtct ttcctttttt gggacagact acaaaacagt
    41641 gattcaacgt gtattacgaa tggatctgac agaatttgaa acacgtccaa cgggcttgta
    41701 tctttttgac tgtctttctg ttcgtgggaa tatctgggtg ttctggcatt caaaacgaac
    41761 tgtaacagaa aatgtacttg tgcaattttc aggacaaggt tgccgggaat atgaggaagt
    41821 catgcgtgaa caacaaacag attggcgaac atggctatat tccatttgga aaaatcaagg
    41881 aaagttggaa gctggttata cacgtgtgca atgtagccga ttcgatattg cgttagatga
    41941 actttgggga ttggaaacgg aagaacattt cgatctcttt gaattactag aaaaacaaaa
    42001 agcaggttta ttagaaatgg actttaagag attccagaat attggcggtt gttttttaga
    42061 tgatggctat tttcgaagtg aaggattatc tttgtatttt ggatcgcgaa agagtcctct
    42121 cttttttaat ttttatgaga aacgttttga aattgcgaac cgagaaaaaa tttcagaatt
    42181 agaagcatta acgaaatatg gtatttataa tcgatatgag ttacggttag ccaatgagaa
    42241 agcaacacaa gcagtagaag catttatttt aggtgattca ccgaaacgtt taggagagat
    42301 tggcgttggt ttaattaatg caaaacttat tgtttgggat agactggaaa gtgatgcacg
    42361 gattgttgat aaacgatggg caaaattatt tcgttcgatg gaagaattgg attttacagt
    42421 aaagccagaa aaattctcat tagatcgttc agaacgctgg tttttgaaac aagtagctcc
    42481 gacattaaag ttgttacaag aagcagatcg tattttagga agagactttg tacaagaagg
    42541 gattgataat gccgaactaa atgacgagca acaaaactat ctagaatgga tgggaacgtc
    42601 aggagggaaa attgcatgaa tgcatatacg acaacagaat ggctgcgcct gttggattta
    42661 aaggctgcaa tcgaagcatt aaatgaaaag atggttgatt tgtcttattt tcgttttcga
    42721 gttccttata ttgaacaagc ggtaaaagct ggacgttatc aggaaaaaga gaactggcaa
    42781 gaaattgcac gcttattaga agttcgtaag ggatacgaac aagaactcga agagttggaa
    42841 tttagccgaa ggaaaggaag gctagaattc attcgctttt atcgattttc attgccaata
    42901 cctgcgattt tagcggttaa aaaaggctgc gataaaatga agatatatga aaactgtgtg
    42961 gcagcactta gcagcgaaaa acctcttatg gaagaaattt cattggtgac tgtcacttgg
    43021 gaaatgagtc gccagccaac agaagaacag acatatcttt ctttagaaga gattgagata
    43081 gaattagagg aaattggtcg ctatgccact tgttctacct attgtggatc ggtcattagt
    43141 attgctggtg tgattgtcta aatgaaaaaa attatgattt atgttggtgc gtattggtca
    43201 cgtgatccaa cagtattaga aaatccagag gcggtacact attgcttgcg ccaattattc
    43261 tatctgtata aagaacgatt agaatcgttg attcgtcagt taccatatac tgacagacgt
    43321 ttagatgaac ttcttctaag gtatccagca atgtataaac gacgaaaaaa tagattgtta
    43381 ccagaagagt atcccattga aaaacatgaa cttgaagggc gatttgtggc ttatttctat
    43441 gatgatgttc gtatgcgctt agtagaacaa cgaatggaaa tagaaaatga tcgctactat
    43501 tttatcaatt attgtaagaa gcgtaagtat caagtaactg atgattttta tgattgtatt
    43561 ttacaagatg gggaagtgat tctatccaaa attgctccaa gttttcgtga acttgtgccg
    43621 attgattttt tgaaaaaatg tcatttgcgg attcttccgt aaaaaagagc tataaaagga
    43681 aagaaaagta gaagccaaat cggtttctgc ttttttattt tttttaggaa ggagaagatg
    43741 gtatgtcact ggaattttta ggagagccaa ttgaaaagga agagttcgtt tcgcattata
    43801 tggatttgtt tattcaggtg ataaaaaact ttgtgcgatt gatgaatttt atccacgcga
    43861 acaagactat ctagccgctg aacgaataaa aatcagttta ttatgggaaa aattcacaga
    43921 tgaaaataac cgaccgccag attatcgcta tttatcagat tgtttaacga ataatgtgct
    43981 ttctaaagaa tacctatttc acaaacgtga gaaaaatcaa atgtcggttg aacatttttg
    44041 tgattgttat attgcattat tgcgtagtca acagttatta acagtagaac gatatggtgc
    44101 agagtttggt tatgtcatgc aagctgagcg tcatagagct acaagatgct atgaaaaaga
    44161 agatcaagtg attgatggct atgaagaatt atgtattcga aacaatcgct atttgcaaca
    44221 acgtttatat gaacaattga tgatggattt ttcaaatctc tatagagggt atgaatgttc
    44281 gatgattcaa gaaaggtaga agaaaagggg gaatttttat gtggcgatta ttatttttgg
    44341 tggtatgtgg tatggcattg ttctatgtgt atcgtgtcta tctctatcca cgaatggaac
    44401 gatggacaag ttggtataga gatcctttaa aacaacgaaa aggattgaag cggtatcgtt
    44461 atgaattact tgttgaattt tcggatgggc gacaagcaca gtattatttt tttgcaaact
    44521 atccaaaata caacaccagt gaactattga ttcaattatt caaaaataat ccatatgttg
    44581 ggttagaaga aaacggacgg gagttctttt atcgttcaga taatattagt gcggcgtcta
    44641 tgaacaaaac acgaattgca taaaaaattg gaggaataat catgaaaaaa acaacattag
    44701 cagtcgtaac aacactagga atttcattat tttcgtttca attagggagt tcattggttt
    44761 atgcagatga aactgtagaa acacctaagt cagaaattgt agaaacattg ccaggggaag
    44821 gattaccaga tgttgagaca gagctaaatg gaggaacaac ggaaaaacca gcagaacctg
    44881 aacaattgca gtcagagaaa cctgaaaaag agccagtgac accagaacca gagtttcctg
    44941 aatcaacttt accgactgat tcgatcgaag aagaaggtgt gcatagttca acaacaacgg
    45001 attcaagtat agtcacaact ccggaacaat caggaacaac cagttcttct gaaaagaata
    45061 ctgattcgtc aagtacggaa cagccaacga tgtcgtctac cagtgaagaa caaaaagaag
    45121 aaccgaaaca acctgttgaa aaatcagaag caattccaaa agaaaataaa cctattgttc
    45181 cagcaaaaga agtcacagta tcggtaaccc caagcggtga aattacgacg aatacttctc
    45241 aaggaatgag tgtaccgatt gtcactagta atgtagagga attgacccat attccaacac
    45301 caaccacccc attaaaagta gcaggtgatc aaacaattgt tggtgtaaaa gatggtattc
    45361 cattaatcca agatagtgaa gggaatttgg taaaggattt gtcgattcct gtaaagaaac
    45421 ttccaagtgg caatattgaa atcaaaacag cagatggaaa aacaaaagtc ttaccaaaga
    45481 ctggtgaaga aatacatgtt gcactttctg ttctaggagg agtgttgaca agtattgctg
    45541 gatttgttgg atataaaaga aagagaaaag gaatagaaaa tgagggtaat tgaatggact
    45601 atttttcaga aaaacaagct tatcgttgtc aatcgtacgt actagtaaaa acatactaga
    45661 aggagtagct tatgaaaaaa cgacaactac ggcggaaaga aaaagtttat acagagaaag
    45721 taccgaagca aaagaaaatc cattcaaaaa aagcagtgaa atggatttgg tgtttggttt
    45781 tatttctttg tttttcaggg ggcttggcat tttttcaatc catgaaaacg aaggttcaag
    45841 tgtcgttttt gaaagatgaa atccaacaat tacaaaagaa agaagtacaa agtcggtcag
    45901 aattaacgta cacacctgaa attgaatcct ttatgaatcg ttttgttgct ttgtatatga
    45961 atacattcga taatcatgaa caagaacaac aacgtcaaga aacattaaaa aaagagtttt
    46021 atgcacaaaa ctttattcaa gaaccaggaa gtgtttcagg tgttcgaaaa ttaactgctg
    46081 cttcgtttat tggattaaaa gaaattgaaa ccattccaac tgctagttat aaagtaactt
    46141 atgatattcg ctcgatgaat ccagagagta agaaagaaac aagtgtgaca atcacacaag
    46201 tactcaatct tcccttttct atttcaaaca agggctgtcg tattattgct tatccttatt
    46261 ttacggcact accaaagaat acagaaaaac aacgaatgat tacttatgat gacacattat
    46321 acgaaacggt tgatgcgtcg gaagcgaaag atgttgaagg atttgtggaa gatttcttag
    46381 ataagtatgc gaaaaacaat gcaaaagata tgtcatacat gatgaaagaa cctgaaggac
    46441 tcaatcaaac agctaaagtt gatcaagtag agatgaaagt attgcgttat aaagaaacga
    46501 aaaaaccttt tattgtaaaa gcaacggtcg tctttcgaga tggtgatacg tcacttttac
    46561 aaaaagaaca aatgacgatt gtcattacga aaaaaacaaa tcagtattat gttgaaaaat
    46621 taacacataa ttggttggat aggaaaggag aagaacggaa atgacactga agggaattgt
    46681 agattttata ttaacgcaag gtggctattt tttaataatg gtggttgttt atctgggatt
    46741 gcgaaatttt atgaaatcaa aagtaggaaa tgcattaata tctatcgcag caggtgccgc
    46801 agtttatttc ttcttaaatg atccaacacg tgtatttgat gcgtttggta aaatcattga
    46861 aaaattattt atggattagg aggatagtga agtggattgg gaaccttatg attattcgat
    46921 tgtttatgcg gaaccttatc gcgtacaaca gattacaagc aattggcgtc ttccatttcc
    46981 agttgcatta gtcaatgcag caactttttt gttggtcgta atctttgaat atttattttt
    47041 acgttcattt attggttggg tcatttcttt ttttccacca gcaggagttg ttttttatat
    47101 aggactcccc tatttatgtg tgttagcact ggcaaaacta aaacctgatg gaaaaaaagt
    47161 gcatttttat ttatgggatt ggttttgtta tatgattcgt atgatgaatg gtgattgtgt
    47221 gtattataac tgggacaagg tatcaaacaa tgcattggaa gaattacttt ttgtgaataa
    47281 gtatgtttat ttagaggatt agaaaaaggg acaatcaact ggttgttcct tttttattgg
    47341 gaggtgaaat tcattggtgc ttgtaaaaga accagtaaaa gcattcaaga aaaattgttt
    47401 gttaaatgga gaggattgta tctgggccta ctatcatgtt tttccacgaa caatttcaaa
    47461 aaacacacca aaaaagacac aggaaataaa agaaatgatg gctcgtttac tgttgctttt
    47521 agcagattat gaagatgtgg agttggtgat gtatccacga acaatgaatt tacggacgaa
    47581 gtttgacaca ttaggaaagg gattttctcc cgatagtcaa gcaattggtg cgtattatgc
    47641 aaaagaaaca gtgacattgt tggaacgaga aggtcaagtc tatcgtcctt catttatttt
    47701 aggaatcaaa gtcaaagaaa aagcaacggt ggagagtttt catgatgtaa taaatctaat
    47761 ctggttagat ttagcaggaa gtttagcacg cttatttgga ttagaagtag atgatttgga
    47821 aactttgttt tctgaaagtg atccttatgt aaaagaagtg acacaattac tttatagctt
    47881 ttctattcgt cccttagtaa aaaaagagct gttgtatctc aatcgtctgc aatatattcg
    47941 aggaatggct cacaaagtag aagaacaaaa taattgggaa gtactgaaag acaatcaagg
    48001 aacgtatgta cgtataccga aggaacataa aggccttctt caattagaag atgagttagg
    48061 gagtagttat ttagcattgt tgcctattgg ggaaacacct gaaaatatga gaaattgtga
    48121 tctatttcaa tttgctcagg atctttcttt cccagcagaa ttgcgaatga aaattcacta
    48181 tccacgcttg aaaggacagg gaggtattgg tttaaaactg aattggctat cggaacggtt
    48241 caaaacagag cataaggaat tggttcaaaa tgatgaagaa cctagtgaac gcttattaac
    48301 tgttcgagga ttagtaaaac atttaaagca acgagtagat aatgggcaac ctgtcgtcga
    48361 ttggatcgca tgttttattg tatacggtca aacggttgaa gaagttcgtc aaaatagtgc
    48421 aacggttatt caacaacttc atacgattat ccatatccac cgtgcaaaaa atgatcaacg
    48481 agaattgttc tataaatgtt tattagggca accattggga cggcagttac attggaagca
    48541 ccgaacaact gtagaagcag ttggcgaatg gttgttttca gcagtatcag aattaggtat
    48601 ggaatccggc tggtatattg gtcggcaaga tacattaggg gatggtggtt ctcgaggaga
    48661 aaaaacacca ttagaagaat taatttatgc gtcaaatcgt tttgtctttt tgaatcttct
    48721 agccattgcg gaaggaatca aaggtgcgtt gtttgatgcg cctcatgtag cagtaactgg
    48781 aaaaaccggt aaagggaaga cctttttagt tggcttgctc tttttgtata gttcgtttat
    48841 gaatgtacaa agtttatttg tggatcccaa aggagaaaag cgcaaatggt ttacgaaact
    48901 cattcaagac ccgtattatc agaaacatta ccctttgttt gtagaacatt tgcaaaaaat
    48961 gagttatgtc acgttagatg ctacaaatcc ccgtaattat ggtgtattag atccaattgt
    49021 ttgtttatcc cgagtagatg cgaaacaagt agcccaggat atgattgatg agttaagtcc
    49081 gttaggtacc aatcaccttt taaaaggggc ggtattaaag ggaatccaag aagttttagt
    49141 tcaaaaagaa gccggacaac aagttggctt gatgcatgtg gtcgactatt tagaacaaat
    49201 ggaacaaaaa gaggttcgag aatatggtga gtttttacgc ttgacggtag aagattcgat
    49261 tttacgttta ggattttctt atggagaaaa cccgggatta gactttaatg ccaaaacaac
    49321 gattttagag atccaagatt tgaaattacc aaatgacaat gtccgaccag aactatatac
    49381 agatgcggat cgaaagtctt tgtgtttgat gattagttta ggtcgttttt gtgaaatgtt
    49441 tggaaaacga gatagtagta agaaaacagc gatttatttt acagaagcat gggtatttaa
    49501 taattcgaac gcaggacgta gtattattgc agcaatggca cgtgtggggc gttcccagat
    49561 gaatcaatta gtgttagata cacaatttat tggtgactta ggttcagata aagaaaaagg
    49621 gaattttggt gtcgtctttg cttttgatga agacagtgag cgagaaaaga ttttgaatca
    49681 tttacgtttg gaagtaaatg aagcgaactt agctgaaatg agccacatga ttaaaggaca
    49741 gtgttggatg ttagatccct atggacgtgt aggaaaactc aatgtacaca gtttgtttga
    49801 agaaatgacc gctgcattac aaacaacgga aaaaacaagt cactctcaag tagaagaaca
    49861 ggatacgtta atcatgcaat agaaatgagg tgaaacaatg gcttatataa agcataagga
    49921 aaagaaatgg tgcgttttgt tttttttaac gattggattc ggactgcttt ttgttggtgg
    49981 attggtgtat gcggcagaaa agcaggtgga tcaatcgatc tatgagattg ggaaatttca
    50041 tattgatacc ttttcttctt atttatctga tagtgaattt tcattaggca aaatgttatt
    50101 tggggcaaat ggcacagatt ttctaaaaat gatgacgaat atgttcttta gtttaagtaa
    50161 attgatttgg caaggccttg attttgtcat tgagaagctc tatgaaggag ctgcaatgaa
    50221 cgagttaatt caccagtttt tcactttttc ggatactatt tattcaaaaa tatacgatcc
    50281 tattggtgcg gggattgtcg tcttgtatgt gttatatcta tttactcttc gtatatttag
    50341 agggaaacat tacgcaaaaa tggtgtttat tcgatttctc ttcgttgtca ttttttcaat
    50401 ggtttggttt ggctatggta gtaacgtacc tagtaaagga gaagctttta ccaaagggtt
    50461 aaatgatttg tccgtggagg tagaaggctt tattttttct gctacaaata gtctagatga
    50521 attggcgaca aaagatgtca atggtgtttt agtgccaacg acacaaaagg aagccattca
    50581 aaaagtgcgc caactttatt ataaagcggc tgttgttgat ccgtatatat tgttaaatta
    50641 tgggacaaca aatttagaag aattgtatca agcagagatt gaaccacaag aatttctagc
    50701 caaggaagcg tcaagtgaag aattatctga tattgagaca aaagtgacca ctgctgcagg
    50761 agaagataga gggaaaaaga aggcttatcg tgtctatatt caaccaacta aagctgttta
    50821 taaagcagtc gttggttcta tggtgccatt gcttaatcta agtattggat taccacttgt
    50881 agtgattggc atgattcgtt ttatctttca aatgttggtg ttgtgtttat tacttgtgat
    50941 tcctttttca ttactcgctt cttttgttcc agtatttgat tatatgctca tgaatacctt
    51001 aaagtccttt ttgggcttga tgtttcaaaa atctttttac agtgtgatta ttttggtggc
    51061 atttttgatc tataacatag tagacgattt aattccaatg aatactgttt ttggtttttt
    51121 aggaaatatg tttgtcaaag cactcctatc aattttactc attgtaaaac ggaaagattt
    51181 attaaagaaa ctagggttag gagctgcaga tcaaactata aacaacacga aaaacatagc
    51241 aagtcattct ttgtctcaaa tgaaacaact gcctggtcaa ctatcaactg ctggacaaaa
    51301 tttggcaatc aaaggtgcag gattagcagg gaatgtgtat cgagcaacga atccactcgc
    51361 ttctgcagcg atacatggat ttcaaaagtt tcaacaaaaa cgtacaccac aagaatcggt
    51421 aaaagggagg caagtggtta atccctttga actaggtggg cgttcaaatc aagccttgcc
    51481 ttatagtggt cgtcatgttc gttatcatgg ggtagagcct gaaatgtctt ctaccaaatc
    51541 gaaaacagag tggaattctg gtctttcaaa tggtttgatt ccaaattcgt ccatgaattc
    51601 agtaagacca aaacaacagg tacaatcatt ggcacaattg agtaagcaac aagggaataa
    51661 tcaaagaaaa ccattgttaa ataaaggtat cgcttttgtt ggtcaatcga ttccgttgcc
    51721 ttctggaaaa gtacccgtgc cacaattaac agctgctggt tctctttcac aacagattcg
    51781 ccaagtgaaa aatcaggcga aaaatgggag ttatccagca atgcagcagt tattgaacca
    51841 atactctgtt cctaattcgt cggtagccac tctatcagga aatcgttctg tgcaacaacc
    51901 ggggcaacgt tcgtctgaaa gtaaaccgtc gatttccgca ccaaaatcgt tacaacctgc
    51961 attttcttct ttagataagc caacagagca atcggaaaaa atagaaaagg atcctaaaac
    52021 actgccgatt ttggacgcaa aaccaggaac tgattttact ttaccaaaag gacgaacagt
    52081 ccaaaaacca ttatcgaaga aaggatgagg aagaaatgaa gaaagggtgt ctaggttgtc
    52141 ttcttttctt tattcttccc ttttttctat tgccattctt tttttcagga gaactttctg
    52201 agcatggagg agaagctaca ggtctagctg tcgcttttaa tggggaatat accgaaggat
    52261 taccgatttt ttcagagatt aaaggacgtg gtccaatcac agatgaacat gcgcaagcag
    52321 cagttggtgc agctgtaaag tatcagttat taccgagtgt gattctttct caattaggct
    52381 atgaatcagc ttatggacag tcacaatctg gaagaaacga tacgaacttt tttgggatta
    52441 cctggtttcc tggttgtccc tacccacaag gttctgcacg tggaattggt ggttctgaag
    52501 gtggtttcta tatgaagttt ccgaacttaa aagcttgtta tgactattat ggctttatgg
    52561 tggcgagcca aagcaacttt aatcaatgtg tgggcaataa agatcctgga agttgtttat
    52621 tgattctagg tcgtggaggc tatgcggcag caggtattac cgaagggagt ccgtattatc
    52681 gaggagcaat gagtatcata acgtcctata acttaacgga atatgatact tttgcgatca
    52741 agcattggaa tagcctacca acaaaaaaaa cttcgcttat tgcaggaaaa agtaaaggca
    52801 atgcgaaggt tttggatcgg atcattggac gatccgtaaa taatggggaa tgttatggtg
    52861 gtacggcatg gtatgtatct caattaggtg gcccacagtt aatgggttct ggacacatgt
    52921 ttgcgatgtt aataggggaa gattataact ggacaaccta tggttggcaa gtaatcatga
    52981 acccaaaacc aagtgattta caagcaggag atgtggtgaa ttggcaagca ggtggtgcat
    53041 tgtctcctgg aatctatggt cacaccggaa ttataacaga tgtttcatat ggcggacaac
    53101 atttctctag tgtggaacag aatgccgagc aaggacggat tatggctcgg tatcaacgca
    53161 cgtacaatca aacaaaaatt cgtagtctgg ttcgtaaggt gaaataagaa aggcagtgac
    53221 tagaaacgtg aaaaaaggga taattttagt atttagttgg ttatgtttga taggtcttac
    53281 tggttgtcat tggttttctt ctgaaaaaca aaaaacacaa ttgagaacga cagaagaaac
    53341 aatcgaagca gtaaaagaaa caagtggaac tcttcaaagt actgaaagta acagtcaaat
    53401 tggttcatta gaagacgaga cagcgcaaca aatgcaaata gatgaaatga caaaaaattt
    53461 cttaaaagat tttgctagaa aatggagtaa tttcacagat gtttataaac gcaatcaagc
    53521 tgtgagggac tatatgacag atcgttgtat taaagacaat agtattgatg tagacccgca
    53581 tgtacaaatg aaaacaactg ggaaacttta ccaggtgaca caagatatag agaatccgga
    53641 tcaatatttg ttatttggag aagaaacaat gaataaggtt actcaatttg tattacttca
    53701 attgaaagta gatcaagaag ctcaaaagat tgatgcgatc aagatttatt atgtacggag
    53761 tgcgtattaa aataaaaaat gaacactcat atttattata caccgaagaa atagaaaaaa
    53821 acagactgtt tttttaagaa aaactatgtc attctttact tgaaaaaagt tgttatagga
    53881 cagaaaggat aaaacaaaaa cggaaaaaat atgtcttatg aataactata agaactattc
    53941 tattttttag gagatggata cgagatgaaa aaactaattg gctatagtgt gatgtgtatt
    54001 ggatttgttg gtgtgggatt gtttgtatcg actgaatctg cttctgcggc tgaatggtat
    54061 aatcctatct ggtataatcc gaacattgaa tgtcaaaaag gctatccgta tggaccgaca
    54121 aatctgaaaa atggaaaatg tagagtaaat tggaatggcg ttgtagatag tgtaattact
    54181 ggttcaatgg actctgctct acatggcttt acgggtcact agaaggagga agaaaatgtc
    54241 tgaagagaaa aaagaaatcg ttgaatcatt agtagcttta aaagattctc tttctaagat
    54301 tgaatatgtt gataggcaag aaattcaaaa actaattgat gatactatta ttgaaatcca
    54361 agatgcgcgt tgtgaaggta taaaaatttc tgtagcttta tctaaagtaa tagaaaaaat
    54421 gaaccgttca ttagcgttta atggattgaa gctagataga caaactagtc tgatatggga
    54481 tcatttaaaa gacttgtatg acaagtctaa aagaagtgaa cggactgctg tatcaatctt
    54541 aaaaggcctt tggggaatga attcataaac aagatattta aaaagtaact tattttttat
    54601 ggaggagaaa ttatgaaaag aataattaaa atatttggtg ttttaggtgt tttcttatta
    54661 ggtttaagta cttttgttag tactggaaca agtgtgtatg ccacagaaaa agtgaataca
    54721 gttgatttag aaataaagaa taaattagta gatgaattaa ctttttattt tgatacagtt
    54781 ggttttttag acgaacatgg tgtataccat ataaaggatt ttggtgctat tcgtgaacga
    54841 atcagtcaag ataacgaaca aggaagagca gcaagagtct tttatgaaca tattattgtt
    54901 cctagaatgg agaaagttga ttggaaacga tatgctggat gtgtagccat taattctctt
    54961 cctggaggag gagtggcttg ggctataggt acgcagggag attttattga agcattaaaa
    55021 gcttttaatt ttgaaaaagc tgcggattta ttattaaaaa ttgcagaaaa acaattagga
    55081 aaagctgcag ctaaagagtt agctaaggta aatattgtag caggaattgc agcagctgct
    55141 gctagctgtg cattagagta aaagtttatg aaaagtttag aacaagagca tatggctttt
    55201 aaacaagcaa tgtttaaaga aaatatctat ttaaaccata actatattcg tgtttctaag
    55261 gcatgttcac cagtattgaa tatgcttgga ggtggaaatg gattatatca tttactgttt
    55321 gtcgatgttt gttggttaat tttttta
//
LOCUS       DACCVU010000005        51414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCVU010000005 DACCVU010000000
VERSION     DACCVU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.005"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            290..1135
                     /locus_tag="IVW29_000248"
     CDS             290..1135
                     /locus_tag="IVW29_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7837758.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IVW29_000249"
     CDS             1416..2636
                     /locus_tag="IVW29_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7837759.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IVW29_000250"
     CDS             2633..5353
                     /locus_tag="IVW29_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7837760.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IVW29_000251"
     CDS             5337..6281
                     /locus_tag="IVW29_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7837761.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IVW29_000252"
     CDS             complement(6350..7360)
                     /locus_tag="IVW29_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7837762.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IVW29_000253"
     CDS             complement(7540..7884)
                     /locus_tag="IVW29_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7837763.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IVW29_000254"
     CDS             complement(7877..8311)
                     /locus_tag="IVW29_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7837764.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IVW29_000255"
     CDS             8449..9186
                     /locus_tag="IVW29_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7837765.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IVW29_000256"
     CDS             9179..10390
                     /locus_tag="IVW29_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837766.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IVW29_000257"
     CDS             10597..11376
                     /locus_tag="IVW29_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7837767.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IVW29_000258"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IVW29_000258"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7837768.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVW29_000259"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVW29_000259"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7837769.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IVW29_000260"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IVW29_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7837770.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IVW29_000261"
     CDS             14978..16027
                     /locus_tag="IVW29_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein"
                     /protein_id="HAP7837771.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVSA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IVW29_000262"
     CDS             16024..16302
                     /locus_tag="IVW29_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7837772.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IVW29_000263"
     CDS             16277..16747
                     /locus_tag="IVW29_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7837773.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IVW29_000264"
     CDS             16764..17066
                     /locus_tag="IVW29_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837774.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IVW29_000265"
     CDS             17083..17454
                     /locus_tag="IVW29_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7837775.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IVW29_000266"
     CDS             17426..17785
                     /locus_tag="IVW29_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837776.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IVW29_000267"
     CDS             17957..18964
                     /locus_tag="IVW29_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7837777.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IVW29_000268"
     CDS             18989..20173
                     /locus_tag="IVW29_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7837778.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IVW29_000269"
     CDS             complement(20610..22193)
                     /locus_tag="IVW29_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7837779.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IVW29_000270"
     CDS             complement(22771..22914)
                     /locus_tag="IVW29_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7837780.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IVW29_000271"
     CDS             complement(23136..23615)
                     /locus_tag="IVW29_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7837781.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IVW29_000272"
     CDS             complement(23688..23972)
                     /locus_tag="IVW29_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837782.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IVW29_000273"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVW29_000273"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IVW29_000274"
     CDS             complement(24316..25602)
                     /locus_tag="IVW29_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7837783.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IVW29_000275"
     CDS             25740..26411
                     /locus_tag="IVW29_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7837784.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IVW29_000276"
     CDS             26377..26865
                     /locus_tag="IVW29_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7837785.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IVW29_000277"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IVW29_000277"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7837786.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IVW29_000278"
     CDS             27826..28590
                     /locus_tag="IVW29_000278"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7837787.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IVW29_000279"
     CDS             28823..31036
                     /locus_tag="IVW29_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7837788.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IVW29_000280"
     CDS             31047..31493
                     /locus_tag="IVW29_000280"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7837789.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IVW29_000281"
     CDS             31664..32923
                     /locus_tag="IVW29_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7837790.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IVW29_000282"
     CDS             complement(33012..33959)
                     /locus_tag="IVW29_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7837791.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IVW29_000283"
     CDS             34353..35645
                     /locus_tag="IVW29_000283"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7837792.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IVW29_000284"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IVW29_000284"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7837793.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IVW29_000285"
     CDS             37538..38413
                     /locus_tag="IVW29_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7837794.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IVW29_000286"
     CDS             38679..40562
                     /locus_tag="IVW29_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7837795.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IVW29_000287"
     CDS             complement(40637..40921)
                     /locus_tag="IVW29_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7837796.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IVW29_000288"
     CDS             complement(40911..41645)
                     /locus_tag="IVW29_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7837797.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IVW29_000289"
     CDS             41774..42397
                     /locus_tag="IVW29_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7837798.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IVW29_000290"
     CDS             42589..43710
                     /locus_tag="IVW29_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7837799.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IVW29_000291"
     CDS             43717..46845
                     /locus_tag="IVW29_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7837800.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IVW29_000292"
     CDS             46864..50058
                     /locus_tag="IVW29_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7837801.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IVW29_000293"
     CDS             complement(50204..50911)
                     /locus_tag="IVW29_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7837802.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16851 a         8966 c        10653 g        14944 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttctgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    51361 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaa
//
LOCUS       DACCVU010000006        49862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCVU010000006 DACCVU010000000
VERSION     DACCVU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.006"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(55..252)
                     /locus_tag="IVW29_000294"
     CDS             complement(55..252)
                     /locus_tag="IVW29_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7837803.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(646..2373)
                     /locus_tag="IVW29_000295"
     CDS             complement(646..2373)
                     /locus_tag="IVW29_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7837804.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2366..3559)
                     /locus_tag="IVW29_000296"
     CDS             complement(2366..3559)
                     /locus_tag="IVW29_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7837805.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3746..4390)
                     /locus_tag="IVW29_000297"
     CDS             complement(3746..4390)
                     /locus_tag="IVW29_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7837806.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4484..4618)
                     /locus_tag="IVW29_000298"
     CDS             complement(4484..4618)
                     /locus_tag="IVW29_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7837807.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4620..6752)
                     /gene="feoB"
                     /locus_tag="IVW29_000299"
     CDS             complement(4620..6752)
                     /gene="feoB"
                     /locus_tag="IVW29_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7837808.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6749..7222)
                     /locus_tag="IVW29_000300"
     CDS             complement(6749..7222)
                     /locus_tag="IVW29_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7837809.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7623..7847
                     /gene="nrdH"
                     /locus_tag="IVW29_000301"
     CDS             7623..7847
                     /gene="nrdH"
                     /locus_tag="IVW29_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7837810.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8006..10165
                     /gene="nrdE"
                     /locus_tag="IVW29_000302"
     CDS             8006..10165
                     /gene="nrdE"
                     /locus_tag="IVW29_000302"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7837811.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10215..11180
                     /gene="nrdF"
                     /locus_tag="IVW29_000303"
     CDS             10215..11180
                     /gene="nrdF"
                     /locus_tag="IVW29_000303"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7837812.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11269..12408)
                     /locus_tag="IVW29_000304"
     CDS             complement(11269..12408)
                     /locus_tag="IVW29_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7837813.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12717..13430)
                     /locus_tag="IVW29_000305"
     CDS             complement(12717..13430)
                     /locus_tag="IVW29_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7837814.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13684..14385)
                     /gene="nagB"
                     /locus_tag="IVW29_000306"
     CDS             complement(13684..14385)
                     /gene="nagB"
                     /locus_tag="IVW29_000306"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7837815.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14619..16151
                     /locus_tag="IVW29_000307"
     CDS             14619..16151
                     /locus_tag="IVW29_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7837816.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16342..17544)
                     /locus_tag="IVW29_000308"
     CDS             complement(16342..17544)
                     /locus_tag="IVW29_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7837817.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17626..18315)
                     /locus_tag="IVW29_000309"
     CDS             complement(17626..18315)
                     /locus_tag="IVW29_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7837818.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18421..18789)
                     /gene="rplL"
                     /locus_tag="IVW29_000310"
     CDS             complement(18421..18789)
                     /gene="rplL"
                     /locus_tag="IVW29_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7837819.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18842..19345)
                     /locus_tag="IVW29_000311"
     CDS             complement(18842..19345)
                     /locus_tag="IVW29_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7837820.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19755..20444)
                     /gene="rplA"
                     /locus_tag="IVW29_000312"
     CDS             complement(19755..20444)
                     /gene="rplA"
                     /locus_tag="IVW29_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7837821.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20548..20970)
                     /gene="rplK"
                     /locus_tag="IVW29_000313"
     CDS             complement(20548..20970)
                     /gene="rplK"
                     /locus_tag="IVW29_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7837822.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21313..21981
                     /gene="sdaAB"
                     /locus_tag="IVW29_000314"
     CDS             21313..21981
                     /gene="sdaAB"
                     /locus_tag="IVW29_000314"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7837823.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21994..22866
                     /gene="sdaAA"
                     /locus_tag="IVW29_000315"
     CDS             21994..22866
                     /gene="sdaAA"
                     /locus_tag="IVW29_000315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7837824.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22957..23976)
                     /locus_tag="IVW29_000316"
     CDS             complement(22957..23976)
                     /locus_tag="IVW29_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7837825.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24149..25036)
                     /locus_tag="IVW29_000317"
     CDS             complement(24149..25036)
                     /locus_tag="IVW29_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7837826.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25113..25658)
                     /gene="nusG"
                     /locus_tag="IVW29_000318"
     CDS             complement(25113..25658)
                     /gene="nusG"
                     /locus_tag="IVW29_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7837827.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25759..25929)
                     /gene="secE"
                     /locus_tag="IVW29_000319"
     CDS             complement(25759..25929)
                     /gene="secE"
                     /locus_tag="IVW29_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7837828.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25953..26105)
                     /gene="rpmG"
                     /locus_tag="IVW29_000320"
     CDS             complement(25953..26105)
                     /gene="rpmG"
                     /locus_tag="IVW29_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7837829.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26267..26911
                     /locus_tag="IVW29_000321"
     CDS             26267..26911
                     /locus_tag="IVW29_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7837830.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26967..27470)
                     /locus_tag="IVW29_000322"
     CDS             complement(26967..27470)
                     /locus_tag="IVW29_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7837831.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27483..27529)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28067..29032
                     /locus_tag="IVW29_000323"
     CDS             28067..29032
                     /locus_tag="IVW29_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7837832.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29131..30303)
                     /locus_tag="IVW29_000324"
     CDS             complement(29131..30303)
                     /locus_tag="IVW29_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837833.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30290..31435)
                     /locus_tag="IVW29_000325"
     CDS             complement(30290..31435)
                     /locus_tag="IVW29_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7837834.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31449..32373)
                     /locus_tag="IVW29_000326"
                     /pseudo
     CDS             complement(31449..32373)
                     /locus_tag="IVW29_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32490..33569
                     /locus_tag="IVW29_000327"
     CDS             32490..33569
                     /locus_tag="IVW29_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7837835.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33601..34194
                     /locus_tag="IVW29_000328"
     CDS             33601..34194
                     /locus_tag="IVW29_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7837836.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34304..34531)
                     /locus_tag="IVW29_000329"
     CDS             complement(34304..34531)
                     /locus_tag="IVW29_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7837837.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34634..37222)
                     /locus_tag="IVW29_000330"
     CDS             complement(34634..37222)
                     /locus_tag="IVW29_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7837838.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37242..37901)
                     /locus_tag="IVW29_000331"
     CDS             complement(37242..37901)
                     /locus_tag="IVW29_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7837839.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37950..38453)
                     /gene="trmL"
                     /locus_tag="IVW29_000332"
     CDS             complement(37950..38453)
                     /gene="trmL"
                     /locus_tag="IVW29_000332"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7837840.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38674..39540)
                     /locus_tag="IVW29_000333"
     CDS             complement(38674..39540)
                     /locus_tag="IVW29_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7837841.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNEVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39649..40290
                     /locus_tag="IVW29_000334"
     CDS             39649..40290
                     /locus_tag="IVW29_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7837842.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40352..41713)
                     /gene="mgtE"
                     /locus_tag="IVW29_000335"
     CDS             complement(40352..41713)
                     /gene="mgtE"
                     /locus_tag="IVW29_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7837843.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41731..42630)
                     /locus_tag="IVW29_000336"
     CDS             complement(41731..42630)
                     /locus_tag="IVW29_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7837844.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42632..43429)
                     /locus_tag="IVW29_000337"
     CDS             complement(42632..43429)
                     /locus_tag="IVW29_000337"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7837845.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43407..44111)
                     /locus_tag="IVW29_000338"
     CDS             complement(43407..44111)
                     /locus_tag="IVW29_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7837846.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44242..44817
                     /locus_tag="IVW29_000339"
     CDS             44242..44817
                     /locus_tag="IVW29_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7837847.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44935..45612
                     /locus_tag="IVW29_000340"
     CDS             44935..45612
                     /locus_tag="IVW29_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7837848.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45691..47499)
                     /gene="pepF"
                     /locus_tag="IVW29_000341"
     CDS             complement(45691..47499)
                     /gene="pepF"
                     /locus_tag="IVW29_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7837849.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47675..48673)
                     /locus_tag="IVW29_000342"
     CDS             complement(47675..48673)
                     /locus_tag="IVW29_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7837850.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48899..49552)
                     /locus_tag="IVW29_000343"
     CDS             complement(48899..49552)
                     /locus_tag="IVW29_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7837851.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14921 a        10469 c         8758 g        15714 t
ORIGIN      
        1 atagatatat cattatataa ttcatgaaca atttgcttag catttttttt cattttagca
       61 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
      121 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      181 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      241 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      301 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      361 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      421 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      481 cgcacccata aattcataat agtttgtcgg cgtctttgga cgtttcgaag atgagctacg
      541 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      601 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      661 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      721 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      781 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      841 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
      901 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
      961 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
     1021 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1081 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1141 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1201 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1261 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1321 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1381 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1441 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1501 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1561 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1621 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1681 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1741 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1801 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1861 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     1921 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     1981 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     2041 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2101 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2161 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2221 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2281 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2341 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2401 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2461 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2521 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2581 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2641 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2701 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2761 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2821 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     2881 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     2941 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     3001 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3061 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3121 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3181 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3241 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3301 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3361 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3421 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3481 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3541 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3601 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3661 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3721 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3781 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3841 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     3901 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     3961 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     4021 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4081 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4141 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4201 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4261 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4321 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4381 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4441 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4501 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4561 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4621 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4681 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4741 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4801 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4861 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     4921 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     4981 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     5041 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5101 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5161 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5221 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5281 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5341 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5401 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5461 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5521 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5581 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5641 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5701 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5761 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5821 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     5881 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     5941 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     6001 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6061 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6121 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6181 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6241 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6301 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6361 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6421 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6481 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6541 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6601 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6661 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6721 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6781 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6841 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     6901 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     6961 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     7021 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7081 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7141 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7201 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7261 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7321 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7381 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7441 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7501 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7561 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7621 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7681 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7741 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7801 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7861 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     7921 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     7981 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     8041 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8101 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc
     8161 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8221 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8281 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8341 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8401 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8461 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8521 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8581 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8641 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8701 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8761 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8821 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     8881 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     8941 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     9001 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9061 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9121 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9181 tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg
     9241 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9301 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9361 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9421 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9481 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9541 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9601 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9661 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9721 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9781 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9841 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     9901 tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg
     9961 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
    10021 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10081 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10141 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10201 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10261 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10321 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10381 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10441 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10501 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10561 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10621 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10681 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10741 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10801 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10861 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    10921 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    10981 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    11041 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11101 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11161 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11221 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11281 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11341 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11401 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11461 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11521 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11581 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11641 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11701 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11761 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11821 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    11881 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    11941 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    12001 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12061 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12121 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12181 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12241 ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat
    12301 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12361 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12421 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12481 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12541 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12601 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12661 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12721 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12781 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12841 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    12901 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    12961 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    13021 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13081 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13141 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13201 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13261 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13321 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13381 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13441 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13501 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13561 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13621 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13681 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13741 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13801 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13861 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    13921 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    13981 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    14041 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14101 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14161 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14221 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14281 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14341 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14401 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14461 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14521 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14581 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14641 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14701 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14761 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14821 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    14881 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    14941 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    15001 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    15061 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15121 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15181 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15241 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15301 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15361 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15421 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15481 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15541 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15601 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15661 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15721 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15781 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15841 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    15901 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    15961 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    16021 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16081 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16141 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16201 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16261 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16321 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16381 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16441 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16501 gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta gcccttgtac
    16561 tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa
    16621 aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat
    16681 aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat
    16741 aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc
    16801 ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt
    16861 tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa
    16921 atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga
    16981 acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa
    17041 atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa
    17101 aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc
    17161 cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa
    17221 aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac
    17281 ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg
    17341 aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac
    17401 tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata
    17461 catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa
    17521 aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta
    17581 tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg
    17641 ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt
    17701 aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc
    17761 acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg
    17821 tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat
    17881 catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca
    17941 atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt
    18001 gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct
    18061 caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc
    18121 tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag
    18181 ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata
    18241 caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat
    18301 tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac
    18361 ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa
    18421 ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc
    18481 ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc
    18541 tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc
    18601 agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc
    18661 agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac
    18721 taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt
    18781 caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca
    18841 attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc
    18901 ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt
    18961 ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga
    19021 tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat
    19081 cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc
    19141 cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag
    19201 catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa
    19261 cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt
    19321 tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag
    19381 ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca
    19441 tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc
    19501 gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa
    19561 aaaactctat gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg
    19621 gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca
    19681 actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac
    19741 attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt
    19801 gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat
    19861 acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg
    19921 attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact
    19981 tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag
    20041 ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca
    20101 acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct
    20161 acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa
    20221 acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct
    20281 gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca
    20341 atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca
    20401 acttttttta atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga
    20461 ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg
    20521 agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt
    20581 tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa
    20641 ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc
    20701 gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt
    20761 tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc
    20821 tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc
    20881 ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa
    20941 tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg
    21001 ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa
    21061 ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat
    21121 ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa
    21181 caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat
    21241 gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat
    21301 gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg
    21361 atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt
    21421 agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa
    21481 acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc
    21541 gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg
    21601 tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa
    21661 ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct
    21721 gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac
    21781 caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat
    21841 atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa
    21901 gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac
    21961 agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta
    22021 gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg
    22081 agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa
    22141 caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat
    22201 gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta
    22261 aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt
    22321 gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat
    22381 cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg
    22441 ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt
    22501 ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat
    22561 caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc
    22621 gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct
    22681 tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc
    22741 gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa
    22801 ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg
    22861 gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac
    22921 gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt
    22981 tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag
    23041 gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg
    23101 tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg
    23161 acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg
    23221 caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca
    23281 gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt
    23341 tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc
    23401 ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact
    23461 ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac
    23521 gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc
    23581 tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa
    23641 ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc
    23701 ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc
    23761 ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa
    23821 ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg
    23881 catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga
    23941 accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag
    24001 ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt
    24061 agatccttct ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc
    24121 aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct
    24181 gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt
    24241 tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta
    24301 acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc
    24361 gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga
    24421 cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat
    24481 taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat
    24541 acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt
    24601 tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat
    24661 tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc
    24721 cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt
    24781 ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc
    24841 cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt
    24901 ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac
    24961 ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt
    25021 tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc
    25081 caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa
    25141 tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt
    25201 tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc
    25261 acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc
    25321 aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa
    25381 ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg
    25441 atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca
    25501 tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt
    25561 ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat
    25621 gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt
    25681 aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag
    25741 tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc
    25801 cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt
    25861 acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag
    25921 aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat
    25981 tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg
    26041 actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt
    26101 gccatttact tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta
    26161 actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat
    26221 gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac
    26281 acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc
    26341 attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa
    26401 tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga
    26461 tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac
    26521 ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag
    26581 tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc
    26641 ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga
    26701 tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct
    26761 tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga
    26821 agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga
    26881 aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat
    26941 ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct
    27001 aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg
    27061 ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc
    27121 atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg
    27181 ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca
    27241 tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa
    27301 agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc
    27361 ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg
    27421 ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct
    27481 cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac
    27541 aataattata acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa
    27601 aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa
    27661 ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg
    27721 tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg
    27781 tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta
    27841 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
    27901 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
    27961 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
    28021 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
    28081 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
    28141 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
    28201 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
    28261 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
    28321 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
    28381 agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
    28441 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
    28501 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
    28561 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
    28621 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
    28681 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
    28741 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
    28801 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
    28861 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
    28921 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
    28981 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
    29041 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
    29101 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
    29161 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
    29221 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
    29281 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
    29341 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
    29401 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
    29461 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
    29521 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
    29581 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
    29641 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac
    29701 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
    29761 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
    29821 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
    29881 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
    29941 atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa
    30001 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
    30061 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
    30121 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
    30181 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
    30241 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
    30301 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
    30361 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
    30421 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
    30481 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
    30541 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
    30601 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
    30661 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
    30721 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
    30781 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
    30841 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
    30901 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
    30961 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
    31021 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
    31081 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
    31141 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
    31201 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
    31261 ccgctttgat tacaattcca gtattaatcg tttcgagatt gccaccttga tcaatctcgc
    31321 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
    31381 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
    31441 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
    31501 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
    31561 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
    31621 attccatcag aagctattgt tcgctgacaa cacgttttct gggagtaatg ggatgtcgat
    31681 cgtcaaacga tccgccgtcc gtaccatatc ttttagagat tctttggttc ctttagcaat
    31741 cacctgtcct tgatccatga tcacgatctg gtcgcaaaga atttctactt cttccatgta
    31801 atgtgttgta tagacaatgg ttgctccttg tcgattcagc tccttgatac tttctaaaat
    31861 tttattccga ctttgggggt ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg
    31921 cttgtgggca atcccacatg cgatgttcaa ccgacgtttc aagccaccac taagttctcc
    31981 tggacgaaac gaaacatatt tttctaatcc cactaattga atcacctctt caatcaaggc
    32041 tttccttttc tctttttcgg aaacatataa accgcaaaaa taatcgatat tttcttgcac
    32101 acttagttcg tcaaagaccg caacgtcttg aggaacgacc ccaatcattg ccttgacagc
    32161 ataattttca ggagtcattg ttgtcccgaa caatttaatg gttcctttgt cataatttaa
    32221 caaagaaagc atacagttga tggcagtcga tttcccactg ccgtttggtc ctaacagacc
    32281 taagacttct ccttcttgta cttgcagatc aaaatgattc aatgctacaa aatctccata
    32341 gcgtttgact aatttatcaa tttcgataat catcatgaaa aactccttcc aaatttcttg
    32401 ttgatctgac tataataaag tgcaagaaaa gaaactagtg acgattggct aaaccacaca
    32461 tgacatttgt cacaatgaag gagtctgcta tgcataaatc tcggctactc aacgaaacta
    32521 gctttctact ggttatctca ttgcttttgc tggtaagtca aggattcgtt gctatgcaag
    32581 ttatctattt attgatcgcc gttttattca ctggtgtcag ttatttactg cctacaaagc
    32641 caaattggat tttccaagtt attctatttc ttttatgtgc ctttttacct gatcttgtcc
    32701 tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga aagccacctt gattacttgt
    32761 ttttgttagg ttttttgttg atttcgacgg ttggtaatca tccccccatc aaggtcgcct
    32821 ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg
    32881 atcttgagaa aaagtacatt gtctccaaag atactcattg ggaaaaacaa caagagttat
    32941 cggaacaaaa taaagcattg gccctgtcac aagaaacaca attagccttg caaattgtgg
    33001 aagaacggaa ccgaattgcc cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg
    33061 cgattttaca aattggtgcg cttgaaatga tcaatcaaga accaaaacta aaagaaccat
    33121 tagaaaattt aagtgccacc gttcacactg ggatggatcg gattcgtcaa agtgtccatg
    33181 acctccatga gacatcgatc tcttttcaac actccttaga gtttctgatc gctgattttc
    33241 cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact
    33301 gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat caaacatagt catgccaaaa
    33361 ccgttgtttt acattttcga acgttgccgg ccttttatcg cttgcaaatc acgaatgatg
    33421 gcaccagccc acaaaaaaat tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg
    33481 ttcaagaaca aaagggacaa gtccatattt tccaagaaaa ccagcaattt cagttaacga
    33541 tcattttacc aagggaggga caccaatgat aacagtagtc attattgaga tgatccattt
    33601 gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt
    33661 ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc atcgtcccga tgttttgctg
    33721 acggacatcc gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat tttggctaaa
    33781 tttcccaaag ccatcctttt gcttttgacg acattcaaag atgaagaata catccgcgaa
    33841 gcattttcca tcggtgtcaa agggtattta atcaaacaga acttgcaagc gattattcct
    33901 tctgtcaaat ccgcttataa tggacaggcc gtatttggta atgaaatcat cgagactttc
    33961 actcaaatga tgaagaataa accgacgatt gatagtcatg cttatgcttc ttttagtgaa
    34021 cgtgagcttg caatcatcaa agaagtagct gccgggaaaa acaataaaga aattgcagat
    34081 gccttgtact tgagtgacgg cactgttcgc aattacatca gccaattact agaaaagcta
    34141 gatttacggg atcgcacgca attggctatc tatttttatc agcatttgca ataagtctta
    34201 tctaacacaa aaaaacctga atgatgctcc ttcgcatcat tcaggttttt ttcacatctt
    34261 ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga ttcgcagggc
    34321 tgccaggatt cgttggattg ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg
    34381 ttttttctag ttttgattct tcacgatcgt ctcgttctac atcaggactt tcattcaatt
    34441 tgaattcttt ttccatctca tctaactttt taaagtcatt gccagattct tttgctaatt
    34501 tcgctaattt atcttcggtt tctttagcca taaagtaacg cccccctttt gatttcagct
    34561 tagcatatca cactgaaaaa acaaaagaaa acgctaacca acgatcattt ttagctattt
    34621 ctcatcttta aaatcaagca ttttttgccg gtataaaatc gtagggagaa ctgttttcca
    34681 taccaatcat cggatctact aactggttat cgatcacttc tgctgtaaga acgaaatctt
    34741 tagaaacagc aggttctacc tcttctttct tttcttctcc aacaaatgaa gaaaacagat
    34801 ctggttccac tgtttccatt acttggtttt tcgtttcatt cgtcccactg tcatcttcta
    34861 caggtagctt ttcttttaca acccgtggct cttttgtata gttcccatct tcaaatggat
    34921 catttccgac caaagaatct tcataagctg ctagtttggt ccggagagtg atattcattt
    34981 tatcattatc catgatcctt gatttcaacg tagtcatccg gcttgctgat tcattcgcta
    35041 cacaagtgac ataggcttgc tcttccccta acaaaatcaa cagttcctta ctgcgagtca
    35101 ctgccgtgta gagcaaatta cgctgaagca tccgctgata ttgatggacc atgggcaaaa
    35161 taaccatctt gaattcactg ccttgtgatt tatggattga gcaacaataa gaaagagtga
    35221 ttttattcca ttcgtttctt ttataagtga cctcatttgc atcaaactgg ataactaatt
    35281 catccacttt atcttcagaa tcttttgcca agatgatacc cgtgatttct cccatatctc
    35341 cattgaatac attcaattcg ggagtattga ctaattggag gactttgtct cctatccgat
    35401 aaacggtgtc gttccaatgt acttcctttt ttccaggttt ccctggattg aatatctcct
    35461 gcatcatttt atttaacgca tcgatccctg caggtccccg atacattggt gctaatactt
    35521 ggatatcttg tgcacggaat cccttatttt tcgctttttc cacaatttta gagatcaagg
    35581 gttcaatttg ataagcatca cttcggaaat aggaacgatc tttttgattc tttgtgaaat
    35641 cttttggcag acgtccttct ttgatctcat gggcaagagg aataatacta gaaccatcac
    35701 cttgccgata aatctgattg agttccattt tgggaatttg ttcgatttcc aaaagatcat
    35761 ggagtacttg accaggaccg acagagggaa gctgatcttt gtctcctact aagataactt
    35821 gcatattcgt cggaatcgct ttgaataatg tgttagccag ccatgtatcg accatagaca
    35881 tctcatcaac aatcaaaagt ccgccttcta attctttcgt actgactgaa ggtgtttttt
    35941 cccgtccatt cagccccaga agacgatgga tcgtactggc aggcagacct gtcgtttcgt
    36001 tcattcgttt agctgctcgg ccagttggtg ctgcaagtag aataggaaaa atttcttggg
    36061 tatattttga aggatccaat tccagtccgt tcaactctgc aaaaagattg acgattccgt
    36121 tgatcacagt agttttcccc gtacctggtc ctcctgttaa aataaataga ggggaacgga
    36181 ttgcttcagc gatagcttgt tcttgcgaag gtccatactg tatgccgaat attttttcga
    36241 tacgccgaat attcttctgc acttcttttt caggataatg gatttctttt ttacgactga
    36301 gaaggcgctg gatcgaatta ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa
    36361 gattagtctc ttcttgttgt atctttcctt cttcaacaag ttggataatt acagtagcaa
    36421 cctgatcagg ctggatttca attgggcgac ttgtttctaa aatacggatc gtttgctgaa
    36481 gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac tgcatgctga aaaatctgat
    36541 gcaagatagc tgctcgaatt cttctcggtg aatctgctgc aataccgatt tgttcggcta
    36601 gattatctgc acgtttgaac ccgattcctt ctatgtcttc gactagctgg tacggatttt
    36661 cttcgatgat atccaaagac tcgttcttat aggcctggta gatactgaaa gctaactgac
    36721 taccgaaccc gtaacgattc agtcccatga tcacttgatc catcccgtgg tttagccgaa
    36781 tcgtctcagc aagcatttct ctttttggct tcgtcaaacc ggagatttgt tttaataccg
    36841 aaggatcagc aatgattttt tcgatagcat cttctcccag caattctaca attttttctg
    36901 ctgttttctt cccgattcct gggaattttt cgctggaaag atacgctacg atcccatttg
    36961 cagttgttgg cttttcttgc tcatatcttt cgagtttaag ctgccttccg tatttaggat
    37021 gatccactaa ttctccataa aaacgataag tctcattttc ttgaatatcg ccgaaacttc
    37081 cggtgactac aatctctttt tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa
    37141 gcactttgta aaaattacta ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat
    37201 aaggcatttc ttctggctgc atcggaagcc gcctccttct tctatagtaa aggttctggt
    37261 gctgtttcag ttcctaaaaa atctgtttgg ttccatactt tcagttcata tggcaataat
    37321 ctgcaaggct cttcagtctc taaaatcgtc aaactactat tgaacaatcc acccatttca
    37381 cgcaactcac ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa agaagctcca
    37441 tgtcctacga ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc tacaacagtt
    37501 tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga
    37561 tctaaatgat tacgcaaatt cggcagatta tccggatatt tctggtacat ttcatcgatc
    37621 gactgacctt ctaaagtacc cagccctagt tctcttagct tatctgtata aaaaatctct
    37681 accggacgag caagctcttt gttgatacct tctgctgtat cacgtgctcg ctttgctgta
    37741 ctggaataaa ttgctttaaa aggaacatat tttacctttt tcccaaatgc tttgatttct
    37801 tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg tctttcctga
    37861 ttccattgtg tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa actcctgtct
    37921 ttcttttatt ttactctata ataaactgtc taatccagct tgtcattttc atagtgatgc
    37981 gtcaattcta aattagggaa tccttgctgg cgaagtgctt catacactat cattgcagct
    38041 gtattcgata aattcaatga gcggacatgc tcatcattca tagggatgcg taaacacttt
    38101 tcttcgtttt cacgcataaa tgtttctggc aaaccggttg tttcttttcc aaataaaaag
    38161 aagtgctctt ctccatcagc aaaattttcg tcactatata ctttatgggc aaattttgag
    38221 atcaaatgta gtttacgatt acctaaaaat gttaaaaaag cttctaaatt actgtggtaa
    38281 gtaatattta catcattcca ataatccaac cctgcccgtt ttagatgttt gtcatcagtt
    38341 gaaaaaccta gtggctcgat taaatgaagc ggagtattcg tggctgcaca cgtacgggca
    38401 atatttccag tgtttgcagg gattaatggt tcaaataaaa cgatatgatt catcaggatc
    38461 attcctctca tatttatgtt cgtttctatt ttctgacaat ttgatttttg cataaaaaaa
    38521 aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct cttctcacta
    38581 acttttatca gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata
    38641 acaccctcaa cttttttttg caacctctac tcattaaaaa ctgctagaaa aacgttttct
    38701 cttagttcct tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa
    38761 gtctgctttg attcgctctt ctacctgcca ttctaaaggt gacatggcca aaagatcttt
    38821 ttgattttcc tctggtatct caaattcata ggttacacgc tgacgttcag ttaatttcat
    38881 tgcttcagca aacttggcta ctacacgttc attggaataa ctctgttttt caggttgatt
    38941 cggataaaaa gcctgacgta gttcctttaa ataattttct tgtggcacca ccttcaacac
    39001 ttctccatct tctgagagta cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt
    39061 taaaacctcg ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag ctacacacca
    39121 aaatgcttcg gcagattgtt ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc
    39181 aacagcattt tgaatctttt tcaattttga aagttcattc aagaagctac cttctccgca
    39241 gccaacatct aatagcgaga cttcttcagg taaatgctgc gcaatagtct caataacggg
    39301 ctgatacatg ccagattgga tcattctacg ccttggaacg aacattcctt gatcgtattc
    39361 tgttttaacc gcatgggata aaaaataaag tgttcctttt ttggaaaggt caaaacggtg
    39421 attatttttg catatcaagc cattatcaga cttttttact tcttcttggc aaaccggaca
    39481 gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat
    39541 agttccccct catttctgtt ttatcttacc gttagatgct cactttggca actgctaaaa
    39601 atatgataca atgcctagaa gaaagtgaaa gataaaggag aagattagat gatcaaagaa
    39661 ttttgcgctg aaaactacac atctattcct ctagcaattg ctagaggagc cgatcggatc
    39721 gaattgtgtg acaacttagc ggtcggtggc acaaccccga gtactggtgt gatcgaagaa
    39781 acgttaagtt atgctgggga aaaagatatc cccgtaatga cgattattcg tcctagaggg
    39841 ggcgacttcg tctataacga tatcgaattg aaaatcatgc atactgattt gattgaagca
    39901 aagaaattag ggacggatgg cgtagtattt ggttgtctta ctccttcagg ctggctagac
    39961 gaagaggcct tagaactatt gatcgaaaca gcagaagggt tgcagatcac cttccatatg
    40021 gcttttgaca gtattcctga agatcggcaa ttcgaagcaa tcgattggct ggtagaacat
    40081 ggtgttgacc gtatcttgac tcatggcggt cctgctgaca aaactattgt agaaaatttc
    40141 ccacggctta aagaattgat tgcctataca gatggccgct taatcatttt gcctggtggt
    40201 ggtgtcactg cagagaatgc agcacatgta gctaaagaat tgaacgtaag cgaagtacac
    40261 ggaaccaaaa tcgtggatct tcagttataa tcttataaac aaaaaaacat ttgcaacagg
    40321 cataatgctt attgcaaatg tttttgtttt tttacataaa atagcccata aataaagaag
    40381 caatattgaa ataaatcagt acactcgtca aatcgctcaa agtcgtgata aagggaccgc
    40441 ttgctactgc tggatcaaat cctagacgat ccattagcat cgggattaaa ctacctgcaa
    40501 gattcgccac tgtgattgca aatagcatag ccatccctat gacaaacccc aagataaaat
    40561 tatgtttcca aaaacctacg attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa
    40621 cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa
    40681 tcgcaagtcg acgaacagcc acagctagcg actgggttcc agcattaccg gcagttcctg
    40741 taatcagcga aatgaagact gctaaaatac tcgcttcact taccaagtct tcatagtgac
    40801 tgattaaaga agcggtcgac atacctaaaa acaacagagt gatcagccaa ggcaaacgtc
    40861 gagacgctgc ctttactgga ttttcattaa tttcttccac attgaccccg gcaagtcctg
    40921 aataatcgct agcagcttca tcatcaataa cgtcgatgat atcatcgact gtgacgattc
    40981 ctaacaaatg atcatcataa tcggtaacag gaagagctaa gaagtcataa tctctaaatg
    41041 tttgtgccac atcttcttgg tcgtcgccta catgaactga aagcactcgt tcgctcatca
    41101 gatcagaaat cattgcgtcg tcatcattca cgattaggtc gcgcagagaa ataaccccta
    41161 ctagctgacc ttcttgatta acgacataga tgtaatagat tgtttcagct acatctgctt
    41221 cgttcttcag cacgtacatc gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt
    41281 cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc ttgatttcac
    41341 tggcatcgtc tgccgacatc aaacttaaat atttagcaat ttgcttttta tccagcgtat
    41401 taagcaagtc aacagcatta tctgtataca tctcagaaag catatcagct gcatagctag
    41461 gacgcatttc atctaggtat tctttcatat gttcgttgtc ttcttcaatc acatcgaaca
    41521 tatccgctaa ttctttaggc gacagatagg aatagatcag ctgcctgttc tcttctgtaa
    41581 gagattggta gaattgcccc tgctcataga tatgcatctc taaaaaactg tttctgaatg
    41641 cctgaatatt attttccctt aaatcttgag tcaactgggc aaatctttcc tctaattctt
    41701 gattttcatc caaaccaatc tcacctcgcc ttattcttca aaccgtctca caactgcctc
    41761 catgtctgcg gctaaaggct gcttgaactc cagcaactct tctgtaaatg gatggtaaaa
    41821 acgcagatca taacaatgaa gggcttgtct ttttattccc agatccattc gcccgccata
    41881 catatcatca ccgagcaggg cacagccaat tgcggaaaaa tgaactctta tttgatgtgt
    41941 ccttcctgta tgcagctgga tatcaactaa cgcgatttct gttagtcgct tctctatcca
    42001 gtactcagtc tgtgccgaac gtccttcatc agagaccatt cgcttcaata gcgaagaata
    42061 atctcgggcg atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa gccttccact
    42121 gactaaggcc tgatattttt tcacaaactt cttttcacga agttgcatat ctaattttgc
    42181 gtgcgcaaat ccatgctttg caaaaagcat caaaccagat gtatcccgat ctagtcgcgt
    42241 caccacatgg attacttggt tttcgtaacc ttgtttttta taataagcct ttacacgatt
    42301 agccattgtc ccatttggat ggtattgggc aggaatagac gaaacgcctg ctggtttatt
    42361 tactaccaga acgtgttcat cttcatacac gatatcgatc ggtgtttcat ccaaaagtac
    42421 ggtttcatgt tctccttcag caggaatcac aatggttacc ttatcatcct tagctagaga
    42481 aaacaagaca ttttctactt gatcattaac tttgatttga ccgccttgaa attttatttt
    42541 cgctaacaga ccctttgaga tccctttttc ttttaaaaaa tattttactt gctgagaatg
    42601 atctttctca taatcccatg taaactgcat atcaatcctc actaataaag gcatctttca
    42661 cacgatgcca aaaatgcata tgtcgataag aagcaaaatg gatccgttct tctgctattc
    42721 gataataaat tgagcggata tttgcttttg cgacattcaa ttgatcaacc gttacaagat
    42781 aatcttctgt attttccaat tttatttcta gccattcatc atgggcaatc acgatcggag
    42841 aacccaaagt acgaaacact cgattattca gtgaagcaat ctctgccaac tgaaaggcat
    42901 tgatactagg atggatcaca gcaccgccga cacttttatt atatgctgtc gaaccagtag
    42961 gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa cagctcgttt ttgatgtata
    43021 catcggctac catcgtacga ttggcccgtt taatcgttga ttcattcagt gctaaaaaat
    43081 gctggtccgg tgtttcatcc aaataactga tccgaacatc cagcaaggga taactaacac
    43141 tttgttcccg gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa tcccgccaat
    43201 ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa acgcacatca ttcagacaat
    43261 gactgtagtg atggaacgcc gacaataatg tgccgtctcc gccgactgag atgaccaatt
    43321 ctggctcatg atcatctcgt tgaatccctg cttgttctaa aagtgcgttc aaacgttctg
    43381 tcacttctat agtctttgct tctttattat gaacgatcgc tactttcatc ccctgcctcc
    43441 tctctttcgc tttctttctt caaaagcgtt cgatagtaga catcatctga ttttcctctt
    43501 ccatgggaga ataaccgctg tgcttcttgg atttcttcac ggatactgga catctcttca
    43561 tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa
    43621 acgccttgat atttataatt cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga
    43681 gtccgaatct ggatttccgc caaaattttc ttctcaccgc tgatcagctg tacaggatac
    43741 tcgatcacca gatgataaga acggtagcca cttgcttttt tatttgtgat gtaatctcgt
    43801 tcttggagaa ctttcatgtc ttttctttta cggatgatct caacaacttg atggatatct
    43861 tctacaaact gacacatgat ccttaacccg gcaatatcag acatttcttc ttctaaacgt
    43921 cccattggaa tatgccgtaa ttgtgatttt gttacaatac tatcgactgg cttgactctc
    43981 ccagtgacaa attcaatcgg gacatgtcga ttttgttcac ggaactgttt gcggatcccc
    44041 cgcagtttca ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa
    44101 tctcgttcca tagctgatac ctctacttca ttaacattca ttgtttattc taccatatat
    44161 gcaaagtttt ttcgtactct tgatttatgc attgaccaaa aaaagaaaat taggcaaaat
    44221 agacaaaaag gagtcgatca aatgacacaa atgcttgaaa tcgaatataa atctatgctg
    44281 acaaagcaag agtacgagat attcatcgct cactatcaat taacagataa agatttccgt
    44341 gtacagacta atatttattt cgataccgtt gatgaacagc tgaagaaaca aaattgtggc
    44401 ctacggatcc gtttcgttgg tcatcaagct gaatatacat taaaaacacc tgctgaaaaa
    44461 gggcgtctcg aaacaacgga tacctttata acagaagaag cagaagcatt tatcttcgaa
    44521 aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa tatcgatcct
    44581 gcttcattaa tccaaaccgg aaagcttaca acaaaacggg ctgaattccc tattgaagaa
    44641 ggacttttag ctattgatga aagctgggga gacaatctac acgattttga actggaacta
    44701 gaggtagggg atgcatctgt gggaaaaatt gcttttataa attttttaaa acgtttttct
    44761 ttaccttatc gtcctgctaa gaacaagatc caacgtatgc ttgaagctaa aaattagaaa
    44821 cctttcattt tcacaaggca cttgagttca tttttttact tttttctgtt aagttatagg
    44881 atgtgaaaga atctagtcac tagttgtata gaatctattt tgggggagat tcatatgatc
    44941 gaaatctatt tatttgttaa tcctttaggg gaatactgtt tcgaaatgga acaacagtta
    45001 ttacaattta tcgaagaaga gtacgggaca ccatcaaaag aaaagatgca atttcgcttt
    45061 cttcctttgg tcaatttgca gacaattggg gatgtcatgc aacaaaaagg aatttctcag
    45121 aacaatcttg aggaacgtaa tcacttattc tcaatgactt attctgcagc attggactgc
    45181 aaagcggcgc agtttcaagg aaagaaaaaa ggccgccgtt ttttgatcaa attgcaagaa
    45241 gcagtcggct gtaatgacat accatattct caagaacttg ctgaatccat atttgaagaa
    45301 atcggcggcg atttgaatat gtttaaagaa gatcgtcagt ctgacttcgt taaagaagta
    45361 ttcttttctg atcaaaaaat tgctcgggaa atgggcatca caaaacaccc ttctgcagtc
    45421 gtttacaatt atgcctgcga ccgtgatttt ggtgttttag tagaagtaaa aggtacaagt
    45481 agcctttgga gaatcacaga gctgtgtaaa acaacagata actcatatca agtttttaat
    45541 ggtgaacatt tacatacaaa tgattctcag ttccattcaa gaactagcca tttacacttg
    45601 ctttctaatt aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt
    45661 tctattcgag aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc
    45721 atttagacgt tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc
    45781 aactcctgct tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc
    45841 caagtagttc tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc
    45901 agcagaaaag ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg
    45961 tgaccattcg aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt
    46021 caatttacca taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc
    46081 cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa
    46141 cccatccaag taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa
    46201 taaatactcc gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga
    46261 gtagtcgccg tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc
    46321 catttcgtgg acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata
    46381 tggatttgtg tcatagcttc ccgaagaata agcatcgctt cgtttccctt tattttcgac
    46441 aacatcgatc caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat
    46501 tggtttcagt gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc
    46561 ttcccctaag accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg
    46621 tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa
    46681 agtatcgtat acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct
    46741 gtaattacgg actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc
    46801 aaatgtattt ctaaattgtt cgtaaacact gtacaatccc ttaaatgctg cttcgcgcac
    46861 ccttcgatcc gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc
    46921 accattttct ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt
    46981 atcacttgaa gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga
    47041 taagacatgg gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt
    47101 tggttcttct ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc
    47161 aaaccaggaa acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc
    47221 ttggtaatct gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac
    47281 ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc
    47341 ttgatcaagt gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata
    47401 tttctcatca aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc
    47461 aggcaattct tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc
    47521 cttagctcta tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact
    47581 tttctactca ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca
    47641 tagaaaaccg attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta
    47701 tgcattcgtt aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt
    47761 cccttttatg ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt
    47821 ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa
    47881 accgactttt ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc
    47941 attgttcttg tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt
    48001 tcttcccatc ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac
    48061 tagtgaatat ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa
    48121 tatgccctga tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat
    48181 cagctttcca gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca
    48241 atgagaattg cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt
    48301 tattcaaata attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga
    48361 tttctattgc tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg
    48421 cttccaaaaa tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact
    48481 gtaagtgctc ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg
    48541 caaaatgagc acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac
    48601 aataatattt ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca
    48661 ttgcaagcag catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa
    48721 aaaagagggc gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag
    48781 ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt
    48841 tatacgaaaa aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt
    48901 atacaaaata aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt
    48961 cattcaatgt ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct
    49021 gattctctac atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc
    49081 gctcttcgaa taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt
    49141 ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg
    49201 cgcttccaga taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac
    49261 tcacatcttc tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat
    49321 ttttactgat aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt
    49381 cgcttcctcg gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt
    49441 cgatttcttt gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt
    49501 catgaccaat tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac
    49561 acctcacttt gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa
    49621 agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca
    49681 agctatctta tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag
    49741 ctaattttcc ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct
    49801 aatcaagaat actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct
    49861 tt
//
LOCUS       DACCVU010000007        48485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCVU010000007 DACCVU010000000
VERSION     DACCVU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.007"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(318..1709)
                     /locus_tag="IVW29_000344"
     CDS             complement(318..1709)
                     /locus_tag="IVW29_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7837852.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1922..2308)
                     /locus_tag="IVW29_000345"
     CDS             complement(1922..2308)
                     /locus_tag="IVW29_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7837853.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2364..2987)
                     /locus_tag="IVW29_000346"
     CDS             complement(2364..2987)
                     /locus_tag="IVW29_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7837854.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3051..4415)
                     /locus_tag="IVW29_000347"
     CDS             complement(3051..4415)
                     /locus_tag="IVW29_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7837855.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTIVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            4688..5266
                     /locus_tag="IVW29_000348"
     CDS             4688..5266
                     /locus_tag="IVW29_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7837856.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            5266..6195
                     /locus_tag="IVW29_000349"
     CDS             5266..6195
                     /locus_tag="IVW29_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7837857.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            6192..7928
                     /locus_tag="IVW29_000350"
     CDS             6192..7928
                     /locus_tag="IVW29_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7837858.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(7991..8827)
                     /gene="pheA"
                     /locus_tag="IVW29_000351"
     CDS             complement(7991..8827)
                     /gene="pheA"
                     /locus_tag="IVW29_000351"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7837859.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(8824..9333)
                     /locus_tag="IVW29_000352"
     CDS             complement(8824..9333)
                     /locus_tag="IVW29_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7837860.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(9326..10621)
                     /gene="aroA"
                     /locus_tag="IVW29_000353"
     CDS             complement(9326..10621)
                     /gene="aroA"
                     /locus_tag="IVW29_000353"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7837861.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(10634..11725)
                     /locus_tag="IVW29_000354"
     CDS             complement(10634..11725)
                     /locus_tag="IVW29_000354"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7837862.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(11742..12908)
                     /gene="aroC"
                     /locus_tag="IVW29_000355"
     CDS             complement(11742..12908)
                     /gene="aroC"
                     /locus_tag="IVW29_000355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7837863.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(12910..13974)
                     /gene="aroB"
                     /locus_tag="IVW29_000356"
     CDS             complement(12910..13974)
                     /gene="aroB"
                     /locus_tag="IVW29_000356"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7837864.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14007..15029)
                     /gene="aroF"
                     /locus_tag="IVW29_000357"
     CDS             complement(14007..15029)
                     /gene="aroF"
                     /locus_tag="IVW29_000357"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7837865.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(15688..16662)
                     /locus_tag="IVW29_000358"
     CDS             complement(15688..16662)
                     /locus_tag="IVW29_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7837866.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(16643..18526)
                     /locus_tag="IVW29_000359"
     CDS             complement(16643..18526)
                     /locus_tag="IVW29_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7837867.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(18544..20769)
                     /locus_tag="IVW29_000360"
     CDS             complement(18544..20769)
                     /locus_tag="IVW29_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7837868.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20884..21918
                     /locus_tag="IVW29_000361"
     CDS             20884..21918
                     /locus_tag="IVW29_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7837869.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(21985..22998)
                     /locus_tag="IVW29_000362"
     CDS             complement(21985..22998)
                     /locus_tag="IVW29_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7837870.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23076..23264)
                     /locus_tag="IVW29_000363"
     CDS             complement(23076..23264)
                     /locus_tag="IVW29_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7837871.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(23704..23832)
                     /locus_tag="IVW29_000364"
     CDS             complement(23704..23832)
                     /locus_tag="IVW29_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7837872.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(23835..25208)
                     /gene="rlmD"
                     /locus_tag="IVW29_000365"
     CDS             complement(23835..25208)
                     /gene="rlmD"
                     /locus_tag="IVW29_000365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7837873.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(25319..26374)
                     /locus_tag="IVW29_000366"
     CDS             complement(25319..26374)
                     /locus_tag="IVW29_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7837874.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(26384..27814)
                     /gene="gatB"
                     /locus_tag="IVW29_000367"
     CDS             complement(26384..27814)
                     /gene="gatB"
                     /locus_tag="IVW29_000367"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7837875.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(27811..29283)
                     /gene="gatA"
                     /locus_tag="IVW29_000368"
     CDS             complement(27811..29283)
                     /gene="gatA"
                     /locus_tag="IVW29_000368"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7837876.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(29283..29588)
                     /gene="gatC"
                     /locus_tag="IVW29_000369"
     CDS             complement(29283..29588)
                     /gene="gatC"
                     /locus_tag="IVW29_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7837877.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(29694..31730)
                     /gene="ligA"
                     /locus_tag="IVW29_000370"
     CDS             complement(29694..31730)
                     /gene="ligA"
                     /locus_tag="IVW29_000370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7837878.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(31747..33981)
                     /gene="pcrA"
                     /locus_tag="IVW29_000371"
     CDS             complement(31747..33981)
                     /gene="pcrA"
                     /locus_tag="IVW29_000371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7837879.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            34136..35695
                     /locus_tag="IVW29_000372"
     CDS             34136..35695
                     /locus_tag="IVW29_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7837880.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(35806..37737)
                     /locus_tag="IVW29_000373"
     CDS             complement(35806..37737)
                     /locus_tag="IVW29_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7837881.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(37755..38669)
                     /gene="pfkB"
                     /locus_tag="IVW29_000374"
     CDS             complement(37755..38669)
                     /gene="pfkB"
                     /locus_tag="IVW29_000374"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7837882.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(38666..39418)
                     /locus_tag="IVW29_000375"
     CDS             complement(38666..39418)
                     /locus_tag="IVW29_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7837883.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(39577..39786)
                     /locus_tag="IVW29_000376"
     CDS             complement(39577..39786)
                     /locus_tag="IVW29_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837884.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(39797..41125)
                     /locus_tag="IVW29_000377"
     CDS             complement(39797..41125)
                     /locus_tag="IVW29_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837885.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(41490..42077)
                     /locus_tag="IVW29_000378"
     CDS             complement(41490..42077)
                     /locus_tag="IVW29_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7837886.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(42160..43410)
                     /gene="clpX"
                     /locus_tag="IVW29_000379"
     CDS             complement(42160..43410)
                     /gene="clpX"
                     /locus_tag="IVW29_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7837887.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(43657..44946)
                     /locus_tag="IVW29_000380"
     CDS             complement(43657..44946)
                     /locus_tag="IVW29_000380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7837888.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(45100..46032)
                     /locus_tag="IVW29_000381"
     CDS             complement(45100..46032)
                     /locus_tag="IVW29_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837889.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(46189..46848)
                     /locus_tag="IVW29_000382"
     CDS             complement(46189..46848)
                     /locus_tag="IVW29_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7837890.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(46851..47876)
                     /gene="sppA"
                     /locus_tag="IVW29_000383"
     CDS             complement(46851..47876)
                     /gene="sppA"
                     /locus_tag="IVW29_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7837891.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(47889..48179)
                     /locus_tag="IVW29_000384"
     CDS             complement(47889..48179)
                     /locus_tag="IVW29_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837892.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT        13257 a        10493 c         8643 g        16092 t
ORIGIN      
        1 atgagaagct attttttgaa attgctcttt gtgactcaga gtgaaacggc aggctcacga
       61 cctctttctt aaacggtgtt caaaagcttg catctgcggt aataagctca accaaacgta
      121 aaatatgagg aactattttt gtttggctgt tcttattact tgatgcaggg cagctttttc
      181 acaacctctt attcacggtg tttcagaagt agcttctcgg aaataagccg aaatttcaca
      241 aaaatttgag aaactatttt taggaaactt cttcttattt cttaaagctg accacttcta
      301 tcacaacctc tttttgacta tgcattcaca tcgatcactt tagaatcatc attgatgaca
      361 tcgcgatcta aatctttgat gcgtgcactt gtcacgatac cagctaccat gctaccgctg
      421 acgtttactg ctgtacgtgc catatcgatt aatggttcaa cagagataac taacccaacg
      481 attgctactg gcaagttcat tgcccctaaa acgatcagtg aagcaaatgt tgcgccccca
      541 ccgactccag ctacaccaaa agaactgatt gttacaattg ccacaagcat caaaataaat
      601 tccaaactaa agacattcac tccagctgct ggtgcgacga tcgttgccaa catagctgga
      661 taaacgccag cacagccgtt ttgtccaata gacaaaccaa aacttgctga gaaattagct
      721 gttgcatcat ctactcctaa agcttttgtc tgagtctcga tattcaatgg caaagctcca
      781 gcacttgaac gtgaagtaaa cgcaaagctc aataccggaa aggctttttt gaaatagttg
      841 attggattga cttttgcacc aatcaaaatc agtgagtgga caatcaaaac agcaagcaat
      901 gctacgtaag aagcaacgac aaatttacct aaattgacga tagctgcaaa atcacttgtt
      961 gccaaaacgt ttgtcatcaa agcaaatact ccataaggtg tcaaacgcaa aacaagcgtc
     1021 acgatccgca taatgatttt atacagactg tcgactaaat ctgaaaagaa tgctgcttct
     1081 tttggcgctt ttcttttcac gcctagataa gcgattccga caaatgccgc gaaaataacg
     1141 actccaatcg tacttgttgc acgactacct gctaaatcag caaaaacgtt tttagggata
     1201 aaggataaaa tctgctgtgg aatcgataag ttttcaactg tttcctgacg tgttgccagt
     1261 tcagagattc gtgctgtttc tgcagctcct tgtgtgaatt tagctccttg gagtccgaac
     1321 agcattgttg aaccaattcc gatcaatgct gcaatagctg ttgttccaag taaagaagtc
     1381 aatacagtcg cactaatttt ccctagcttt tcagaaactt tcatttttgt gaatgcacca
     1441 atgatcgaaa taaacactaa aggcataact agcatttgca agaatgatac atatccttga
     1501 cctacaatcc cgatccattc cattgattga gtaacgactt tgtcgtctac accaaagatc
     1561 aattgaagca gaccgccaaa gacgatacca actcctaatg ctgtaaagac gcgtgttgaa
     1621 aattttgtat ggcgcttttg catacgatag aacacatata ataatgccgc aaaaactaga
     1681 accactaaaa ctgtgatgag tgttgtcatt tttctcctcc tcatattatt aatgacaagt
     1741 ccatcgaagg aagtaaaggt tcccgagaat gattcttcaa ccatcctcct gaaaaaattt
     1801 ttttcagtga cttttatatt ttagacgatt tttatcgaaa tgacattctt tttgcttaca
     1861 attagtaata aaaaaacata cctgtgatag ctttaatcta tcgcaggtat gtttttactg
     1921 tctattcttg tttttctgat tgaaaattca attttacccc aggctgatct aaggcgatct
     1981 ctgtcacctc ataagacatt cggcgtacga tatcgaataa cggtgctact agctcgtcca
     2041 cttcttcttg tgtgacatca ttttgacctt caattttccg attgaggata tgattgatct
     2101 gactcacact tccagataaa acatatgtat caaacactaa acggaactct aagcgtgctc
     2161 ctacgatgct atgttctttc ggatagtttt cgatcgtttc ttttaaaggc gagaagccta
     2221 ctttcaaatc tgtttctact tcttggttcg gcaatctcat atcataatga aatgcttcta
     2281 caacttcttt ttgtcttctg atttccatat aattttatca ctcctttatg aaaattataa
     2341 catatctcct ttgaaagggg atactacttt ttaatgatgc tctgcaaggt aaggatttca
     2401 ttcagatttc ctaccacata atctgccggg atctcaccca gaaaatgatc gatgtcgtaa
     2461 agacaagtag cgactcctgc atttttccct gcttcgatat ctaaacgacg atcgccgatc
     2521 atcatcgtgt ctgttctttc taaatgaaaa gtatcgatca aataatttaa agatgctgga
     2581 tcaggtttac gaggaaagtc ctgatcgatc cctactactt cctcaatcag atgtgccaat
     2641 cgatgttctt ttaacaggcc ccatgttgat tctgtcaaac ggtgggtcag gatgaaatga
     2701 cgaccgccgt tgtcttttag catttccagt acttgtttcg tttctggaaa tggacgggaa
     2761 acttccgtac tttctttttc atatcggtga aataattcat taaactctct ggcattcaag
     2821 tttgattcat tgatcaaata actagtagaa tactttttca ttttgaagta gatttctttt
     2881 ttgtccatac tgataccaaa atcttttagt gcttgccatg caccatctac cattgcggga
     2941 tacgtatcaa acaatgtacc atcaaagtcc catatataat ttttcatgtt ccttttgttc
     3001 cattcctttg cttctttttt ctatctctca cttttagata aaggtgttct ttaaagtatt
     3061 tttttgtcca gaattgtcaa atcatctgct aatgtatact ggattggttg tgcatttggg
     3121 atatcgatcg tatccatttg atcttcagga acatcttcta aatattttgt cagtgctcgg
     3181 atgctattac catgacccac aactagtaaa tcttgccgat cttttagttt tggtgctaga
     3241 tgatcttgcc ataagggaat gatccgattc actgtcattt ccagactttc tccgtatggg
     3301 atcaaatgcg gatccaagtg atcatatctg cggtcaaatc tattttctgc ggttaacggc
     3361 ggaactatcg tagatactag tccgatttct ttcgcaagag tgcccatcag ttcttcattc
     3421 gaagcaaacg ttgcttctag gaaatctttc gctgaatctt ctccagctac tcctttgatc
     3481 acataccagc cagcgccgcc aaggttagaa gcttgcgctt tttgcagatt ttctggaagt
     3541 gcaggtattg gtgcaatctt ccattttcca gattggtttt ctgctccttg gatcgttgaa
     3601 gaataccaag cgccagttgg gctagaagct actgctcctg attgtatcgc agttactcca
     3661 gcattccagt cagaggtttg ttcgactaga tcttctttca aaagagtggc aaacaattca
     3721 agaccatact ttaaagatgc attgtcttgg atcgtgactg tctccccatc ttcagatgta
     3781 taccattctc ctgcctgttg cataatcatc cttactcgac caaggtcaga aggattgact
     3841 tcagcgattt tctttcccgt cttttctttg acatctcgcg ctacttgaat atactcgtcc
     3901 cacttcagat tctccatatc ctcttctgta taacctgctt ctgccatcag atccgtacga
     3961 tagaagtttg ctgttacgcc cgaatcgaaa ggaacaccgt atattttatc tcctactttg
     4021 tttaccgcaa atttatacgg tgcaaaatga tcttccttta caatagcaga caaattagcg
     4081 aatgcatctg gataagaagt caaatagcct tgtatcctat aatcttcaat caagacgata
     4141 ttgggcaatc cttctgtatt cccgctcgct aaagcggtgt tcagtttctg aacgatatca
     4201 tcctgagaca tcgtaacaac ttctatctct gtttcttcgt tctcataaac tttttttgct
     4261 tcattcactg ctttgatatt gaatgtctca tcccacgccc agacagtgac tttttcttct
     4321 ttgtctgctg tactactagc acttccgcat cctgttaaga aacctgatgc tgccgctaaa
     4381 caaacgactc cagcaattaa ctttctgatg ttcattttta tccgctcctt ttccatccat
     4441 tagttgttta cgtttgctac attcatcata ccaaaatttg aaaacggttt ctttattatt
     4501 cagatacttc aaaaatttct cctaaaataa taaaaaaata caaatcaact atgacaaaag
     4561 gaataatctt agcatctaga ccaaagatcg aaatggtgag gtttctttaa aataaaaata
     4621 tccttgcatt ttctcctaga aataagtatt tttaaattac aagactattc ttaaggcggg
     4681 taaaaaaatg ccaatacgcg taattcttat cgatgatgaa cagacgattt tagatggaat
     4741 gaaacagttg atcaattggt ctgagcacgg gtttcaatta atagggactt tttccagtcc
     4801 gctgaaagcg cttgattttt ctttggtaca tgcaatcgat atcgtggtca cagatgtaat
     4861 gatgcctgaa ttaaatggaa tcgaattatc gcggctatta aaacagcaac aacctgaaac
     4921 acagatcctt atcctctcta gctatgatga atttgatatg gttaaagatt cttttaaaga
     4981 aggcgtcgct gattatcttt taaagcctcg attgagtcct gatttttttc taaattcctt
     5041 aactgccgtt tcacaaaaaa tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta
     5101 tgagcagatc tgcgagaatc tttcacagca gattgctgga caaactcgtc cattgcttga
     5161 ccctcttctt tttgaacaca ccttgttttt ctgttatata ccgaaaaacg gccattcagt
     5221 tcaaaacaag gctctaaaca aaaacagtcg atcacccatt cattgatgga tagcgacgat
     5281 tcgtttacta tttatcctgt ttctaccttt tcggaagaat ccggctatgt aatcaatact
     5341 gattccaaag atacacttag gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt
     5401 gatttttttg ttgtttctga ttcttttgca atccaagata tcaatgaaat attccaagag
     5461 atcagaaatt gcaccaaagg acaacgtttc tatcataagg gaaagaacct tgcttatcag
     5521 catgaacttc ttccgttatt aaccaaccac gagcagcaag caagtagtta tttaacaaaa
     5581 atcgttcata aagactacgc tttagctgtc aaagaacttg agaatttctt cacgacgata
     5641 ttggaacagc ctattgatcc agcttatttg aaacatgagg caatcaatcg tttctatgca
     5701 ttactcaacg ctttggctga cgagcatgtt gggaatgaag aactttcttt gctcaagatc
     5761 gcccttcctt ctttattagc cgatgctgag tatttcgaag atttttctat tcttgtcaaa
     5821 gaaacacttg agaaactcac ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc
     5881 ttgactttga tctacgagtt cgtccaggaa aattatgaga aagaaatcaa tttacaactt
     5941 ttatccgaaa aatatcattt ttcatatagc tatctctcag ccatctttac tgaaaaattt
     6001 ggtatcaact tttcaaaata tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg
     6061 acggaaacac ggcttacttt gacggaaatc tgtgagaaag tcggctatac cgaactgggt
     6121 tatttctcca gagtttttaa agaagagaca ggaatgacac cttctcaata ccgtaaagga
     6181 acttttgtaa aatgagaaaa aaaatcaaag agcatgaact ctttgccaaa cttctgttca
     6241 ttttgttcat cggtcttttt ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat
     6301 caagggatgc ctatatccag ctgttcaatc aatcgaatga tgtggtacta aaaaagattc
     6361 aatctgaatt tgaaagtttg aatgatacaa tcgagaatac attagccgct tttgattcta
     6421 atccagctgt caaaagctat ttttccaatg atccagccca acatatggaa cagcttcagc
     6481 aactaagaac aatccaaaaa atgaatgact ctctttcaaa aatccatcca atgatcgatt
     6541 acgatgtatt gatctttgga gaaaatggaa gaacttttgt cggaaacgac atgctcactg
     6601 ccgtatctgc cgattctttt tttcaatctg cgattgctca gagagtgaat gaacgtgctg
     6661 ctgatacaca aatgcttttt gcacaccacg gattgactct tcgcgataag aaagcaccca
     6721 gcgtcttctt tgtcaaaaaa ctgaaaaata cattgaatca cgtttacgga tatgctgttt
     6781 tatcgatctc atccaagcaa ctagcgaatc tttttcaatc tgtagtaaat cctgagatat
     6841 cgaagatctc cttcatgaac gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa
     6901 ttgggagccg atcaatgatt ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg
     6961 gacagcagtt gacccgtctt cctctgtacc gccaggattg tacattactc agtcaagtcg
     7021 atcttcattc tttatctaac caaatgggcg tgatttttcc gattattttg tataatatcg
     7081 tgacacttgc ttttgctggc tcattggtat ttatgtattt ggatcgacac acaaaatcga
     7141 tctaccgctt gattgattcg ctaaaacggc tagaggaaga tggcttaaaa gctactgttc
     7201 ctgtacaagg aagccatgaa gtccaattgc tagggaatac gatcaatcaa ttgctaagta
     7261 atgctcagca tcattatgaa cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa
     7321 tccaaactat gcaagcacag atacagcccc attttattta taatacgctg actagcttga
     7381 aatttttgat ttggcagaaa aaaacagatg aagcagttca aggaatcgac agtttcatta
     7441 ctttactaag aagtacgatt gggaaaaaag aagaagtgat tccagtcaag gaagaaatca
     7501 aaagcgtaca aagttacatc gatcttttga gtatgcgata tggcgaaaga atctcggcta
     7561 aaataatgat tcctgatgaa ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa
     7621 ttatcgaaaa cgcctatctt catgcttttc acaataaaac gcaaggattc atcacggtgt
     7681 acggcaaaac aaaaaatcag cttcttttat tcgaagtgat tgataacggc gatggcttcg
     7741 atactgaaaa taccaaacaa aaaaatgatc atttttcagg aatcggcatc agccatgtag
     7801 atgagcggat acgcctaatg tatggcgatg catatggctt aacgatccaa tcagtgattg
     7861 ggacaggaac gactgtaacg atttgtctac cgcttcttcc taaaaatcaa tcagaatatc
     7921 aaaaataaag aatagccaag cgctgatttt tttattttaa gttaaataaa tcaatcagta
     7981 ttcttgactt tcatctacgc gtctcttcta ctgtcagcac tggataacag cccaatattt
     8041 gactttctcc tccgagcatc ttgatttctt ggagcgcatt ctctactaat gtcattggac
     8101 ggtccaacaa caaatcgatc acgaaaaaat attctcccaa gctagttttc aatggtcggg
     8161 attcaatctt acttaagttg atttcccgcc agccaaaagc tgccagcatt ttatgaagca
     8221 tccctggccg attagcaggt agtgtcaaga tgacactcat ttttactgga gctgactgct
     8281 ggctggtcat ctttcggtca ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt
     8341 ggatattctc agctaagatc tccaatccaa catgctgtgc tgtttctaat gaagcaatcg
     8401 ctgctgcgtc ttctttcgga tgttctgcta catacattgc tgcagcagtt gttgattctg
     8461 ttgctactaa aggaacatta ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg
     8521 cttgtggatg tgaaaggatt tttgtaatct ttgttgctgg attgcctaaa agctgctgct
     8581 tgataggaag aacgatttca cttttaactt cgacttctgg atgtttggaa agaagatcga
     8641 ttgtgtgatg aacagctcct tccaatgaat tctctacagg aacgacagcc agatcgactt
     8701 gtttgcgaaa caaagcgcgc aaacaaacag ggatggaagc atacgcagtc aattgttctg
     8761 taggaaatag ctgacaagcc gcctgataag tgaatgaact ttctggccct agatagctga
     8821 cgttcatatg cttttgatcc tttctataat ggtatcgatg atcttttctg gtgtttgatt
     8881 ggtcgtgttg attcgatgtg tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa
     8941 tgcttttatt tcttctcttg tttttccaag agctaaaggt cgaatagacg tttcatcttg
     9001 ttggatacga tcaactagac agtccgcttc tgcctctaag taaagaacaa tcggctgtgt
     9061 ttttaaaaaa cgccgatttt ctgcactttg gacaatccct ccaccagtcg cgataatttt
     9121 gtcttcattt gacagctttc ttaaaatatc tgactccact tttcgaaagc ttttttcacc
     9181 gtaatagtga aaatattcgg caatcgacaa ttggatatgt tcctcaatct ttgtatctaa
     9241 atctacatac tctttttgta aacattgagc taacccttta ccgattgttg tttttccagc
     9301 gcccatgaaa ccaatcagca aaatgctagc catgagcttc tcctcctgac acaagttctg
     9361 ctaaatcgtc aaaaaatgcc ggataagaga ttgaaacagc ttcagctcct tctagttcgc
     9421 aaggtccatc agctatcaaa gcagctactt gcagcatcat accgattcga tgatcgcctc
     9481 tgctggacac ttttgcgcca tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg
     9541 tcgcttttat ctttgctccc attttctgta attcttctgc caccgcatca attctgttgg
     9601 tttcttttac ttttaattct tctgcgtctt tgatgatcgt gattccttct gcttgagtag
     9661 ccactaaagc tagaatgggc aattcatcaa tcagacgcgg aatgatttct ccaccaatcg
     9721 ttgctttctt caaatgactc gtcttgacac ttaaatcagc cgattggttc tgttcattgt
     9781 gattcgtctg ggtaatcgct gcgcccatct cctccaacac atctaagata ccagttcttg
     9841 ttggattgac cccgacattt tttaacaaca gttggctttc cggaacaaga agtcctgctg
     9901 ctagaaagaa agcagctgat gaaatatcac ctggaacagt tatctgttga ccagttaatt
     9961 tttgcggtcc ggtcactatg atcgttttat cttccactgt gatccttccg ccaaattgac
    10021 gaatcatttc ttccgtatgg ttacgggaac gttctttttc aacgattacg gatgtgcctt
    10081 ctgcttgtaa tgccgcaaac agcaaagcag atttgacttg cgcactggct acaggcatat
    10141 tgtatcggat aggtgataaa gagcgggttc ctgtcacagt gattggtaga tactggtcat
    10201 tttcttctcc ttgcaagtca gcgcccatct cgcgtaaagg ttccatcact cgccccattg
    10261 gacgtttaga caacgaagca tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa
    10321 tacccatcaa taaacgtatg gtcgtaccgg agtttcccat gtcaattgat tcggttggtt
    10381 ggacaaatga gtggctaccg cgaccatgaa tcttgatcac ctcttcctct tcttcgatct
    10441 cgacacctaa ttgttggaag gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat
    10501 tttgtatagt cgtcgttcct tcagcaattg ctccaaacat gatgctgcga tgggaaattg
    10561 atttatctgc tggtatccta acagtccctc ttaatccatg tatttgttgt aataattgca
    10621 tttatccagc ctcctatttg atccagcaat gataactggt attagacata atgattcttt
    10681 ttgcttgtaa caaatcctgt tggtttttaa aactgatttg cagtattcct aaaatatctt
    10741 ccctggtctc ttggatcttc aaattaatca aagaaactcc cgcttttccg atcagtccag
    10801 ttacttctgc aataacgcca ggttcatctg gcacatcgat aaataaatca aaaaaggcag
    10861 gaatcgcgcc ttgcttatgg acaggcaact ggtctcgact ttcttttgcc cgattgaaaa
    10921 aagaaaagat ttccgattgg ttctcagtca tgatccattc tgctatttgt gtcatttctt
    10981 gttgccaatc tgccaatcgt tccaataata tctgcttatt gctcaacaaa atatctgtcc
    11041 acatgacagg atctgaggaa gcgatcctag tgatatctcg aaagcctcct gctgccagtt
    11101 gtttggctcg cggatactct tgattgaatt gttctgcctg attgactaag ccagctgcca
    11161 gaatatgtgg aaaatgactt agcattcctg ttatacggtc atgttctctt gctgtaagag
    11221 ttacatattt tgctttggtt ccgcaaaata gttcttgcag tttttcaaca tcttttttcg
    11281 tcttttccaa aggtgtaaag atgtaatagg cattttcaaa tagtttagca tctgctgcct
    11341 gtatacctga tttatgagaa ccagccattg gatgtccacc gacaaaccga agatttttct
    11401 gctcagcgga tgtcacgact tgctgcttcg tactgctgac atccgtaatg atgacgtgat
    11461 cagacaagtt gattctttca aggacatcaa gataagctaa ggttgtacgg acgggaacgg
    11521 ctaataagat aacgtctgct tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa
    11581 cgatctgctg ttctactgct tgctcacggg tttttgactg actatcccaa ccaactacta
    11641 tcagttctgg attggtctga cgaatacaaa gtgccataga agcaccaatc agtccaagcc
    11701 ccacgataaa aacatgttgt gccatcttat ttgccccctt tttagtaatg tttcaagtag
    11761 tcacggtact ccttcactgc agctttcaac tcaacaagcg aatcacttgg gaattttgac
    11821 aatagctctg tcgctaccac agtggctaca acattttccg caacgacgct agctgcagga
    11881 actgctgtac tatccgaacg ttctacactt gctttgtaag gttcttttgt atcgatatca
    11941 acactttgaa gtggcttgta caatgtagga atgggtttca tgaccccgcg aatgacaagt
    12001 ggttcgccat tggtcattcc gccttcgaag ccgcctagat tattggtacg tcggaaatag
    12061 cctctttctt cttcccatag gatctcatcc atcacttctg agcctggtaa tgaaccagaa
    12121 gtgaatccga ccccaaattc tacgccttta aaggcattga tactaaccac agcttgtgcg
    12181 attttggcat ctagcttcgt gtcccattgt acgtagcttc ctaatccagc tggaacgcct
    12241 ccaacgagca cttctacaat tcctccgatc gtatctccat ttttctttgt ctgatcgatc
    12301 aaatctttta ttctttgctc aactgctgga tcaacaactc gtacttctga cgcttcagaa
    12361 atttctttga tttcttgaat cgagatattt ccaggtattt ctgcttggat tccaccaaga
    12421 caaatcacgt gtcctgctac gatgatgtca agttcttcca gaattttctt ggcaattgcg
    12481 ccgattgcta cacgcatggt cgtctctctt gcagaggacc gttcaagcac attccgaaga
    12541 tctcgatgat ggtatttcat accgcctacc aaatccgcgt gtcctggacg cggacgtgca
    12601 actcgccgca gtgattggtc tttttcaggc acttctgcta cagacatgac agttgtccaa
    12661 tttttccaat ctttattttc taccactaaa gtgacaggtg aaccaattgt tttgccgtga
    12721 cggatgccag acgtgatccg aacacgatct ttttcgatct tcatccttcc tcctctgccg
    12781 taaccagctt gtctgcgagc gagttcgtga ttgatttctt cttctgacag aggtaatcct
    12841 gctggtagtc cttcaataat ggctgttaat tctggtccat gggattctcc cgcagtcata
    12901 taacgcatct tacagtcctc ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat
    12961 ttttgcttgc ccaatctttt ctagcaagat gatattcaat tggcttcctc ttgctttctt
    13021 atcatgggtt atcgcgtgga ataattcatc tggatgactt agatcgtacg taatcggcag
    13081 atggaatttt tgaagtatcc tttctaaagc agctgtcgat ccagttggag acaagccgat
    13141 tttttctgca tgagaagtga tggctaccat tccaatagct actccttctc catgactgac
    13201 taccccatat cctgctgtat tttcaattgc atggccaatt gtatgaccaa aatttaaaat
    13261 aagtcgattt ccttggtcaa agacatcttc ttccactact tttcgtttga tttccaacgt
    13321 agcagtaatg attggaacag catgttccag aagatctttt tcgtctttca gctcgttcag
    13381 ctgtttccaa agcgttgcat ctgctatagc tgctgatttg atgatttcag cgattccttc
    13441 tcgtaaacga cgtaccggca aagtttttag aacctcagga tcaatcaata caccagctgg
    13501 ctggctaaat gttccgacaa ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc
    13561 aatactagaa tctacttgtg caagtagcgt agtcggaatt tgaacgaaat ggatcccacg
    13621 catgaacgtc gaggccacga atgctgctag atcacccacc attcctccac caagtgcaag
    13681 tatcccatcg ctgcgggtaa aatcattatc tgctaaaaaa tcgtataact cagctgcttg
    13741 ttctaaagat ttactggtct cacccgccgg tacgattgaa aggactggtt caaaattagc
    13801 ttgtctcaat tggaacatga ttttttctgc atatagtgga gcaacgtttg tatcagtgat
    13861 cacagctact ttttgaggtc tccaaatcgt ttgaagccat gatccagcct gttctagtgc
    13921 gcctttttca atatgaatat cgtatttttg gttatcaaca tgtacagtaa gcattcgttt
    13981 cacttctttc ttttaaaaag ctggaattaa tcgagtgtct gaatcttttc cattgcttct
    14041 ttcatgatcg caacttcttt catcatgcgt gagtaagtct tttcattcaa cgattgggga
    14101 ccgtctgacc aagcatttgc aggatcagga tggatctcta cgatcatgcc atctgctccg
    14161 cttgccacac ccgcacgagc cattggtggt acaagttccc aaattcctgt accatgactt
    14221 ggatcaacaa tcacagggaa atgagttaat tttttcatca atgggacagc acttaaatca
    14281 aacgtattac gtgtcgctgt ttcgtaagtc cgaatcccac gttcgataaa aatgacagga
    14341 gatttgtctt cgactgcaat gtattctgca gcattaagcc attcattgat cgtaccggaa
    14401 attccacgtt tcaatccaac aggtttacct gttttaccga cagcagatag cagtttaaaa
    14461 ttttgcatat tacgtgcgcc aatctgtagg atatcgctgt actcagccac catatcgata
    14521 tgcccttcat ccatcacttc agtaatcact ttcattccaa attcatcagc cgcttgacgg
    14581 atgtatttca atccttcttc ttctaaacct tggaaagcat atggagaagt acgtggtttg
    14641 aaggcaccac ctcgcagaat ttttgcgccc ccggctttgg ccattcgcgc acattctctg
    14701 atttggtcta atccttcgat agaacaagga ccagccatcg tcacgaaact gccgtcacca
    14761 atttttactc cgtctacatc cacgactgta ttgccaggat ggaattctcg agaagtcaat
    14821 ttgtatgtgt ttaaaatacg cacagcattt tctactcctt catagcgtgt aaatgctcct
    14881 tcttgtaaac ttcgcgtatc accttttagt ccaatcacta ctcgttctgt tccttgatcg
    14941 attgagactt tgaatccttc tttttcgata tgatcgacga ctttttttat ttgttcttcc
    15001 gttgcttctt ttttcataat tactatcatt gtttttcccg ctttcttttg tttacttatc
    15061 tatcttggct gattttctct actttttcaa ggtggtttta cattaattcc tactaaatga
    15121 ccgtgtcctg ttgttctcat tttcgctcct tctttcctac ttatgaccca gataatcaac
    15181 aaaaaaagat gtccctgtag gaaattgcct ctacagtgaa catcttcaac gagccttaaa
    15241 aagcgctgca aaaatataaa atagccacta tagatgaatt tctctacgtg gctgtaagtc
    15301 gtgtttaaaa atctttagcc atcatagata caaacctggt ttgtttgtac ccacgaagtc
    15361 atgaatacaa acctatctaa aatagctaaa gtaatacgta ttcagttttg taagttttct
    15421 agatttcata aaaaatcgct ccaaactgat gtaagaattg tacctagtat agacataaaa
    15481 aaatcgactg tcaatcattt ttatgaaact ttttttaatt ttctgatagt taactttgtt
    15541 tttcataaaa ataacaattg gttttattga agaaaatagt catgaacaaa agtatctgag
    15601 aactttttta ctctcttgtt catgacatat agaataaagc agctttgtct tgtaaactcc
    15661 gtttttcaaa taataatcta aagattgcta ctgaataata aagccgattt catggtcctc
    15721 ttcgctgctg attcggtaag gtgactcaac atccgctccc cagctattga tacccccgac
    15781 tcctcttact tttgcaaaaa tcgtaagaac cgttcgtctt tctggcggta gttcttctat
    15841 atgtgttgcc tgttccagtt cttctgcttt gaatggtaaa gcggaaaaag caaatggcgt
    15901 atcctgctgt tcaaatcgca acgaaaaatc ttcttgatct ttatccgtat tatccaacgt
    15961 ttgatttctt gttagttcta cccatttcgt ttccatatgc atgccgcatt cttggggaac
    16021 aaggtatggg gaaacgggga gtcctgccac tttataaata ccttcgacac caccagccat
    16081 ccgatccgga tatgtttccc ctgataggcc ttcatactca aaaaattttg ctttcgttgg
    16141 cataatgaac cttaaaccca atgcgggtaa ttccggtaat cctttttgtc cataataatg
    16201 catattgaca agcattttac cctctccagc gatttggtag gaaattgtca cttgggtttt
    16261 tggtactgtg ttgatttcca gtgtaaaaat gatttcaacc gtttcagcgt attcattact
    16321 tgaatattga ttatttactg gagcaatcgg caaatcgatc tttttattgt ccaccagtac
    16381 ttctatttct ttgacttttg gatacaatcc ggctcctaac cacataccgg atttaaatcc
    16441 aaatccgcta cctctgtcat tatcggtcgt tgctcgccaa aaaactggaa gtgtttcccg
    16501 gtatagccat tcttttccgt ttttattgag cgattccagt ccgcctcttg tataagaaaa
    16561 aatataatgc gtattccccg atttgacacc taatgtacag tcgccgaaga cgattgcaac
    16621 tttttccgta tccatatgac ttttatttac tgtatttttc ataataatat cttacctcct
    16681 gcatggcggg tttttcttgt ccatctgcaa aaacgatccc attccctgag aattcatagt
    16741 cacttggttt atcgtcaaag tcaccgccat agcgcatcac ttctctgcct gtgattggat
    16801 cagtcacttg gatcgcctga tctttaaaat cccatatata gcctccttgg tacatttcat
    16861 aacgatcaag caacgtcatg taagaatcca ttccacctaa cgaattcccc atgtcatgca
    16921 tatattcaca taagatgaac ggtttttccg ctttattttc aaggtagtca atgatctctt
    16981 ctggtggcgc atacatgcag ctttttacat ctgaaatcga atcttcgaat gctggatttg
    17041 gaaaattccc ttcataatgg acaagtcgat cggggtctac ttttttgaag taatcgttca
    17101 tttgtcgaat gttttcttcc gcataagatt catttccaag agaccaaaaa agaatacttg
    17161 gatggttttt taatgtttcg aaattcgttt ttgctcgatc aagaacagcc gcctgccact
    17221 gaggtactgc acctggtacg ttgtaagaag gctcaaatgc gcccattttt tgccatgagc
    17281 catgggattc gaggttcgtt tccgccatca catggattcc tgcttggtcg cataaataat
    17341 accaagtgat ctggttgggg taatgagatg tccgtacgcc gttgatcttt gcatttttta
    17401 tgatagccaa atctttttcc atatcttcta tagtgatggc gcgtcctttt ttcggatgcc
    17461 actcatgtcg atttaccccg ttgataatca tccgttttcc attgaagtag atgattttat
    17521 ctttgatttc gattttacga aaaccgaagg aatagtcaat atactccgtt actctttttt
    17581 cttcgtctat cgtctccaat tcaagcttat ataaattggg ctgaaaatgc ccccacagtg
    17641 ccacattttc aaatccatcg atgcgaatcg tcatttgctc ttttaacgga tattgctgtt
    17701 gatagatttc cttcccatca ggatctagta tcgctacatt cagctgactg tttttggcta
    17761 cttgctcaaa agacaaatct acttcaagtt tgccattttg acatttgtca tctaagcgag
    17821 gtttgatcca aagatcttct aaatggtttt gtcctcgagc aactaagaag acatctctga
    17881 agatcccaaa aaatcggaaa aagtcctgat cttccaagaa tgcagctgta cttcttttgt
    17941 aaacctccac agctagaata ttttcttctt ccaaataagg tgttaaatca aattcagacg
    18001 gagtgaacga atcttcggca tacccgataa aatgaccatt caaccaaata aacattgctt
    18061 gttctacacc agcaaaatga atcgagattt gttttccctt tagaccttct tctaatcgaa
    18121 aagtcgtcaa atacgaacca accggattgt ctttagcagc tgaaaagatc ccttccgcct
    18181 ctttctcagg gcttgtcgtg aaagggggac ggcgaaatac atgtccctcc cacggataca
    18241 tcgtattgat gtaatggatc ttgtcataat ttgctaattc aatatgacta ggaacagtga
    18301 tttcatcaaa agaacaggca tcaaaatctt ttttataaaa tgtttttggt cgtaccgaag
    18361 gatttggcga ataacaaaaa cgccatgatc catttaaaga ctgtttaagg gaggatgtgg
    18421 tttctacttc ttcccttgtt gcataacaaa catgatcgct atgtgcattt gattggttca
    18481 ctctaaaaac ttgtggatca tctaaccatt ttatatttgc tttcatactt ttttccttct
    18541 ctctcataac tttttgaaat gaagcagttc tgtttggaaa tcaccttgaa aatcggggaa
    18601 gtaaagtcca actgtcatca attcggcccc cgaaaatatc cgcttcgagg tctgttcctg
    18661 ataaagtgcc tcatcatcta accccatcag atatacttca tggaacgctg gttgagctga
    18721 agctagtatc ctccctaaaa acacaatagc ttcatcttgt ttaggagaca caaacatcca
    18781 tgcggcaata ttttcttcaa aaggactcgc caaacgataa taatggccat actggatcac
    18841 cggacgaatc gtttgatagg atataatttg gtcttttact tgcttttctt cttcaggaga
    18901 cagctttgtc aaatccagtt cataaccaaa aacagcgctc atagctgttt ctcctcttgt
    18961 tttcaacgaa gtagatctgt cggtctgatg atttggtaca gctgatacat gagctgtgat
    19021 ggacgaaatc ggataagcaa ggcttgttcc atattggatt ttcatacgct ccactgcatc
    19081 cgtgttatca cttgtccatg actgcggcat ataataaata aacccggcat caaaccgtcc
    19141 acctccacca gaacagcctt cccataagat ttccggataa tcagttgtca aatgttcaag
    19201 tagttcataa agcccgagca tataacgatg cgctacttct ccttgtctct gtgggtcaag
    19261 ggtaatcgaa tagacatctg aaagcgaacg gttcatatcc catttgatgt agtcaagagg
    19321 aatcgtatct aaaatctttc tcatttgttt ctcaatcgtc tgccgaacat ctaaccttgt
    19381 aaaatccagt acatgttgtg atctggaagc agaaggcatt ctacctggct cctgcataag
    19441 aaaatcagga tgtgttcgat acagttcaga atcaaccgag atcatttcag gttcaaacca
    19501 taaaccaaat ttcaatcctt tgctatgagc ataatctgct atttcacgta atccatttgt
    19561 caacttgcct tcatactcga accagtctcc taatgaagaa ttgtccgcat ctctttttcc
    19621 aaaccagcca tcatctaata cgaacatttc gatgcctagc tcctttgctt catcaatgat
    19681 cgccttgatt ttttcactat tgaagtcaaa gtaagtggct tcccagttat tgaccagaat
    19741 cggccgttca gcaaattgat gacgacctct tgcgattctt tcacgtagca actcatggtg
    19801 gatctgactc attttgttca atccctgact tgagtaagaa aggatcattt caggtgtttg
    19861 aaaagactgg cctgcattca attcccacga aaaacgatgg ctgttgatcc caccgacgac
    19921 tctcaattga ttgatctgat ctttttcaag ttcgaaagaa tggttgccgg aataaacaaa
    19981 ttgaagtccg atcgcttctc cagtattttc tgtcgtatga tgatgaacta aagcaataaa
    20041 gttattcata tggtgactgc tcgttcctcg acggctttca aatacttttg ttccagataa
    20101 aacagattgc cgtgaaatct gtctttcatt gacatgtgct cctggcaaag caatgacttc
    20161 atcaaatcgc ctagtatggg caaagtctag ctggaaacta gccgcttttt ctagtttgat
    20221 tgtttctccg gtttcatttc ttatctttac agatcgtgtg atcactgctc tatcagtaaa
    20281 tatggtatag aacaaatcga aataaatcgc tgtttctcta tccttaagtg aaatcaccaa
    20341 cgtttgtgcc tctgacttgt ccatcacata agtttgaggc aagccttcga tctctggctt
    20401 cccatctgta atataatggc tgtcatatct cagatccagt aagtctgatc cattcttgcg
    20461 ctgaatgatc gatgcgggcg ttcgaaagtc catgctgcca tgagaagagt attcttgcgg
    20521 tagtgtatct ttcgacagag accggtcagg atttaaggga acatttcctg aaaatccacg
    20581 atctcttcgg ggataatttt tattgtcttt gtactgcttg actggtttgc caaaataaat
    20641 atgcgccaat acattgcttt cttctatctt tatcagataa gaaagaaatg tatttcgtaa
    20701 atggaagact cggctctttt catcaaataa gatgaaattt ttatctgttt ttctatgcgt
    20761 aatatccatc atttcactcc caacaaaacg gaaaccgttt actttttttt ataattcctg
    20821 tatactgttt actaaacaat tttgtcaata aaaatttttt atttttttga ggagtgtgta
    20881 aaaatggttg gaattagaga tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc
    20941 cgtgtgttga ataacgatca gacgatccat gtggctcaga ctactcggca acggatagta
    21001 gaggcagctg ctgcgttgga ttatgacatc agtactcgta aatatatcaa aaagagaatg
    21061 ccttcgattg gtgtgatctc tacgatcggc aaagcgagcg aagaacagga tatctattac
    21121 aaacatttgc gagtagggat ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga
    21181 atctataatc tatctgataa tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg
    21241 atcgttgttg ggactgttac gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa
    21301 atagtcgtcg tggataaccc tgacatccag caagaggtcg atatggtgta tgcagatctt
    21361 gaacggatga ctaaaaccgt actggaattg ttcttagaaa atggtcataa aagaatagcc
    21421 tatatcgggg gatataaagt cgatatggat gaattcggtg ggaagcagat ttctataaat
    21481 gaaaagagga tgcgtgccta tcagcatttt atgatcgatc atcacttaga aaaatatatg
    21541 tcttatcatc taggtggctg gacagaaaaa caaggtgagg tacttgcgac taatctatta
    21601 gaaaatgaat cccctcttcc tactgccatt cttgtcggga gcgatccaat ggcaattggt
    21661 gtctatcggg cattgaaaca aaaaaatatc caaattggag aagagattgt tgtagcaagc
    21721 tttgataata tcgagacaat cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat
    21781 gcgaaatcaa ttgggcaagc agctgttcga atggcattgg agcgaattga cggtatacgg
    21841 gacgaaccaa tcattttgac ttatcctgca cgattgattg ttagggaaag ttttatccca
    21901 agaacaaaaa ggtattgaaa gagaggttgt gaaaagccgc ttcgtttctt catactcaaa
    21961 acgaaacggc tttttcacaa cctcttactt atacttattc acaaaatcca atgtctgctg
    22021 gtaacaattt tgcgattctt ttagcgtgta atcaaattgg tattcatggg taatctcttt
    22081 ttttgtatct gtataaaaca aggattgcac gggtaccttt aattctgtca accgattttc
    22141 taatgcttga ccttgctcct ggaatgagta tgcatttccg tcagttatat acgttggcgg
    22201 aaattcctta gaaacatggt cagccacact agcttctttc aattgctcac tcgttttcca
    22261 atcttttgtt ccaatcaacg accacgcaac ggttttgacg aaaaacttca taaatcgatc
    22321 atccgaatgc tgctgtgctg ttagcttcaa atcaacaggt ccgcaataag aaatggttcc
    22381 tttcagacta tcttttggaa gtagctgttt gattttcatt tgtttagcat acgcatcatt
    22441 cgtttgtata gctgcatatt gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa
    22501 taattgttcc atgtctagca atttgtcgtc tttttcttca gctaataaat ctttgatcag
    22561 ttcattcact tgaagaactt gattagggta ttctgaatca ggcgctaatt cataattcat
    22621 cgcaaccact gcgatgttgg cgtctgaaac taattttgtc gcaaattctt tgacacccga
    22681 tttatctccg ccgacgaatc ctccaccatg tacccaaaat agaacaggaa caggcccctt
    22741 gctgtttttt ggatagtaga tgtcataagt attctcttta tacttggact catacgtcaa
    22801 atctgttttt ttcgccacgt ttttttctgc ttgatcgaac gtttttttat cagtgatttg
    22861 aacagtattt gaaaacatat ggttgatcaa gaatgctcca ggtctggggg aaagctggaa
    22921 ggcaagaaag attgcaaacg cgatcccaag aatgattccg aagaaccatc cgataaattt
    22981 aagaattttc tttttcataa ggctccctca gtagttagta ttaagcattc tgattatacc
    23041 atagtgttat ttcctatcaa gctctcctag atgatttaat gattttcccc aaacttcttc
    23101 tgcaatgctt ttgacaaaag aaattttttc cattgttctt caaacaaaac attttcttct
    23161 tctgttgaag caaatccaca ttgaggactt aagcacaaac gatccaaagg cagatatcgt
    23221 gtcgcttcgt agatccggtc aatcacttgc tggtgctctt ccatttcgcc atttttcgtt
    23281 acagtcaaaa tctaagacaa ctaatttatc ttcagagact ttctctaaaa gaacaaaact
    23341 cccagcacgt tcactatcgt attctaaata ataggcacgg acattttctt gagtaaaata
    23401 atgggtctgc tacgtcgtca tcccccctga tgccgccaaa atcgaataat aatttccgcg
    23461 tcaaacatgt atatttagtg ttgaatcatt tgagaatatg aaaataaaaa tctttttgat
    23521 caatcagtta atcaaattga aataacgaaa aatctgagac agaatgacgt tcattctgac
    23581 ccagatttga ttttgcacaa ctcaacagtc cttattggca aatcgccaaa ataatgtaga
    23641 ctaactaaaa agactaagaa acaataacaa aagttaacac ttcttagtct ttctttagca
    23701 cgtctattta ttaaagattt tagggccttt tctcatgttt ttacgatcat tcttaggtgt
    23761 ttccatttgt ttggatcggc ctccaccttg tttctttttg atcaattctt tcattttttc
    23821 tttttggtcc atttttattc tcctttaacg aatgaagcga ctaattcgat atgatgggtt
    23881 tgcgggaaaa gatctacagg ctgaactttc ttcacggcat aacctgcttc tgcaaataac
    23941 ttagcatctc ttgcaaaagt tgccggatta catgagatat agacgattcg ttctggtgac
    24001 atttcgattg ctgattcaat aaaatgatga tctaatcctt ttcgtggagg gtcaactacg
    24061 atcacagatg gtttgatccc tttttcttgc cattttttca taactttttc tgcttttccg
    24121 acttcataat gaacattttc gattttatta tttaaagcat tgaactgtgc atcttcaatt
    24181 gcttctggaa taacttccat cccatacacc ttatccactc tgtctgctaa agacaagcca
    24241 atcgtaccta ttcctgaata agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac
    24301 tcgatggctg tttggtacag atactctgct tgttgagtat ttacttgata aaatgacttt
    24361 gctgagattc gataggtttt tcctaatagc tgatcttcga tatagtctct gccatataaa
    24421 actttttctt tgtctcctaa aatcacattt gttttttctt cattgatatt ttggatgacc
    24481 gaaaccactt ctggcaattc ttcatgaata gcttcagcaa tttccttgga tttaaagaat
    24541 ttttctttac gagtaactaa aacgaccatc atttcatggg aataatgacc acgacggata
    24601 atgatgtgac gaagctgtcc ttcatttttt tcttcattat atcctcgtac ttggaaccgt
    24661 tgcaagatat ctctgactac tttgattgct tgatcgatga caggatcttg gatataaaaa
    24721 tcttcgatag ggactagatc atggctattc tttttgtaaa aaccggtggc taactgtcca
    24781 tcgatacggc gaacagggat ctgtgcttta ttacggtagc cgaatggttc tgccattccg
    24841 accgttggaa gtacaggaac ttctggcatc ttagcaatct tcgccattac atgctctact
    24901 tgtttttgtt tgaagacaag ctgttgttca tacttcaaat gggctaaagg tgcgatacct
    24961 atgcgaagta atgcattgtt ttctacaggc tgacgatcag gtgattgtgt aataaatgtt
    25021 tcgacttttc catatccaaa cttattgcct gcttttacta ctagtatttc tacttcttct
    25081 ccagggagtg cattttcgat aaataatgga taaccatcga tttttgctac ccccattcct
    25141 tcataactca agtcgacgat ttcgactgta tatcgttcgt ttttctttac tatttgctgt
    25201 tctgtcatat tttgctcctt taagcctatt tttcaatcct actgaactct actataaatc
    25261 agctaattac acaactaaaa ccggcactga ccttactacg ttcagcaccg gcttttgatt
    25321 aattcttttg attaggtgtt tcgtctgcta tttttccgtc accgtcgata tcttcatccg
    25381 ttaaacgttc gacttctttt acgaattctt tgctgacaac ctctaattct tcttcatctg
    25441 tacctgtgat tgcttcatca ggaatctgat cggtattggc aaagaactca atgtgttgat
    25501 gaagattttg gaaatgcatc ggtgcttccc cgccgtattc accatctaag ttgatcatta
    25561 ctggtcgatt gatatcatct tctgtttcaa cgaacaaact gcttgttttg gtatagatta
    25621 ttcttggatc atcgacatgt cgaccgccat tcagcatgag tgctgcaata tgcaagatct
    25681 caaagatatt tgctgtcttc acaatgatta aagaaaactt cccatcatcc aatttggcat
    25741 ctggcgcgat ctgttcgaat cctccaacag agttcgtcag ccctaggaaa aacatcgaag
    25801 catttccgat atattcccct tcatcatact tcaagcgcat tttgattggc tttactcgag
    25861 ggagcatctc tgctcctttc gcaagataag caagataacc aaaaatactc tttagttctg
    25921 atggaacttc ataagtcaat tctgtcaaat agccacctgc agcgatattg atgaagtaac
    25981 tgttatcaga aaggccgatg tccattttta cggtttggtg tttttttatc acttctgctg
    26041 ctgctttaat attatctcta ggaattttta atgctcgagc atagtcgttc gttgtaccag
    26101 ctgggatgat ggccatttta ggacggtgtt caagcggagc gattccatta accacttcgt
    26161 tgattgtgcc atcgccacca gctgccacta ctagatcaaa acctagttct gcgactctac
    26221 atgcttcatt cttggctgaa tctggttctg gtgtagttgc ataggcgctg gcttcaaatc
    26281 ctgcttcttc taatgcctgt aggatatccg ccaaattttt tttcaataat tcttttcccg
    26341 aggtggggtt ataaattacg cgtgcttttt tcattttcag cctctatcgt ttcgctaatt
    26401 catctttaag tagcttattc acgacaccag gatttgcttg tcctttagtt gctttcatga
    26461 tttgaccgac taggaagcca accgcacgat cttttccatt tttgaaatca tcgacagatt
    26521 gttggttgtt atccaaaatt tcattaatga ttggcagtag tttgttcgga tcagataatt
    26581 gcacccaacc atttttttca actacttctt gggcttctcc gccatttgtg atcaattcac
    26641 ggaagacttt tttggcgatt tttgagctga tcgttccatc ggcgatcaaa cgaatcattc
    26701 ctgctaagtt ctcaggtgtc aattttgtct cagctaattc cagtttctca ctgttcaagt
    26761 aagcagaaac ttcacccatc aaccagttag atgcttgctt ggcatctgca ccattttcca
    26821 aaacagcttc aaaaaaatca gacatttctt ttgtaagtgt caataccatt gcgtcatact
    26881 ctggaagtcc aaactcacgg atatagcgtt cgcgacgaga agctggcatc tcaggcaaac
    26941 tagcttttac tttttgaatc cattcgtcat cgatcacgaa tttcgggata tcaggctctg
    27001 ggaagtaacg ataatcacta gacccttctt tgacacgcat caaaactgtt ttattcgttg
    27061 tttcgtcaaa acggcgtgtt tcttgttgga tttctcctcc tgaaagaaga acttttgctt
    27121 gacgtttttc ttcaaaagct aaaccttttt tcacgaaaga taaagagttc aagtttttca
    27181 attctgcttt cgtaccgaac tcttcttgtc cataagggcg aagagaaata ttggcatcac
    27241 agcgcataga gccttcttcc attttcacat cgctgacttc tgtaaattgg ataattgatc
    27301 gcaatgcttc tagataagcg tatgcttctt ctggtgaacg catatcagct tcagaaacga
    27361 tttcgatcaa aggcgtgcct tgtcggttca aatcaacgta agaataaccg ccaataccat
    27421 gcatgttttt tccagcatct tcttctaaat ggacacgttc gatccggatt tttttctttt
    27481 taccttctac ttcgatttcg atccatccgt catgaccgat tggttgatca aattgggaaa
    27541 tttgatatgc ttttggatta tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt
    27601 tagagatttc acagtttaat gccaaagctg ctttcatccc gaattccagc gcagctttat
    27661 tcattactgg cagcacgcct ggataacccc agtcgatcac gtttgtattg ctgtttggtt
    27721 ctgcaccaaa atgagctggc gctggagaga aaatttttga gttggttttt aactcgacgt
    27781 ggacttctag tccaatgact gtttcaaaat tcattcgtct ttccctccta aaatagctgg
    27841 ttgttgttta tggaattctg ttgcttgttc aaacgcatat gccgcttgat acatcgtttt
    27901 ttcatcaaaa taattaccga tgatctgcaa tccaactggt aatccttctg ataaacctgc
    27961 cggtacagac atacctggaa gtccagccaa gttgacaggg atcgttaaga tgtcactcat
    28021 gtacattgtg attggatcgt taatgttctc accgattccg aatgcaactg ttggtgaaga
    28081 tggcccgatg atcaagtcat aatctgcaaa gactttttca aaatctcgtt tgatcaatgt
    28141 acggacttgt cctgcttttt tgaagtaagc gtcataataa cccgcgctta aggagaatgt
    28201 acctaacatg atacggcgtt ttacttcttc accgaatcct tctgaacgtg agttgacata
    28261 tacatcatct aatgatttca cattttcaga gcgataaccg tagcggatac catcgaatcg
    28321 ttgcaaattg gaacttgctt ctgatgaggc aatgatataa taaacagcca caccgtattt
    28381 tgaatgaggc aagctgactt cttccacaac agcacctaat gaacggaatg tttctgctgc
    28441 tttcaataca gcttctttta cgccaggggc cacaccttcg cctaagtatt ctttaggtaa
    28501 agcaatcttc attcctttga tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac
    28561 gcctgaagaa gtaccgtctt tttcgtcata accgctgata gctgtcaatg ctaatgcatt
    28621 atctttcacc gtacgggtca ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa
    28681 accaaaacgt gagacacggc cataagtagg tttcatccct acgatgccgt taaaagctgc
    28741 tggttggcgg atacttcccc cagtatcact acctaaagaa actggaattt gtccagctgc
    28801 aactgctgct gcagaaccac cagaagagcc accaggtact tttgttgggt cccaagcatt
    28861 ttttgttttc ttgaaataag aagtttctgt acttccaccc atagcaaatt catccatgtt
    28921 tagcttacca acagggatca tatctgcttg atagatttta tccatgaccg ttgcatcata
    28981 aattggttca aaattgtaaa ggatcttaga tgcagcggtt gtcaagatat cttttgttac
    29041 gatattatcc ttgataccaa ttggaatacc agcaaggacg ttgctttcat caatcccttt
    29101 ttcatctaaa gcttttgcct gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc
    29161 gacctcaggc tctgtttctt tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt
    29221 gatttctttc gatacaagca tatgatgcag ttcttctaga gaacgattgt gtaattctgt
    29281 cattatgcgc cagcctcccc attatcgatg attgcgggta ctttgataaa gccgtcttct
    29341 gtttccggca catttttcaa taattcatcg cggttccatc cagcttgcgc ccgatcttgt
    29401 cgcatcacgt ttactgtttc aaccacattc gacgtaaatg gaacaccttc cgtatctact
    29461 tcttccagca tttcaaccat atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt
    29521 tcttcatctg cgaacgcaag ttttgctaat ttggcaacgt gtttcacttg ttcttcacta
    29581 atcgccatac taccccttct ttctaaacga gttaaaaact ttgctctcta ttttaacaca
    29641 agagtgccgt gtctgaaatt gtttattgaa atttgtctga aaaaactcat ttttcattct
    29701 tctactgagc gacttttagc caatgcatct gccatttgat cttctgtcca aacttcgatt
    29761 cctaattctt gtgctttggt cagcttgctg ccagcatctt ctcctgctac cacgatatcc
    29821 gtttttttcg atacgctacc agtcactttc ccgccaagat tttcgatcgt ctcttttgct
    29881 tcttcacgag taaatcgtgt tagtttacca gtaaggacta ctgttttttc tttaaaagga
    29941 gactcgactt cttgcaattg ggcatttcga agccctttgt attcgaaatt gacgcctgct
    30001 tgttttaatt cattcatcaa ttcatggact tcttcgttac tgaaataagt gactacactg
    30061 tcggcaatga tatcaccgat cgtatcaagc gcaataatcg actcatagtc acttttactc
    30121 aatgtttcta gatcaccgaa atgttctgct aaaatttttg ctgctttcgc tccgacatgt
    30181 cggattccta acccgaaaat caaccgctcg accgaattat ctttgctgtt gtcgattgct
    30241 gtcaaaatat tatttgctga tttttctttt attttgtcca acgtcaataa ctcttcttct
    30301 gtcaatttgt aaagatcagc aacatctgct actagttttt tatcatacat ctgttctagt
    30361 acgcgcggcc ccaacccatc gatattcatc gcatttcttg aaacaaagtg gttcaaacct
    30421 tctttcattt gagcagggca tttaggattg atgcaccgta aggctacttc ttcatccaaa
    30481 tggacaagtt cacttccaca aactggacaa tgtgtaggca attggtattc ttcgctgtct
    30541 ttaggtcgtt tatctaacac gacttgcgaa acttctggga tgatgtctcc cgctttataa
    30601 atcagaactg tatctttcaa gcggatatcc ttcaacttaa tataatcacc gttatgcagg
    30661 ctggcacggc ttactgtcgt tcctgctact cggactggtg tcatgatagc tgttggcgtt
    30721 actacccctg ttcgaccgac tgtccattcg atgttttcga ttagcgtttc aacttcttct
    30781 ggcggaaact tgtaagctgc tgcccatctc ggtgctttga tagtaaaacc aagttgatct
    30841 tgtagcgaga attcattcac tttgatgacg attccatcga tttcataggg caaatccaca
    30901 cgtttgtcat gatattcttc aatgtatgac cacacttcat caatcgaatg acatagacgt
    30961 ttttcccgat tcgtgtggaa tccaattttt tctagctctt ctagcgcatc atactgggtt
    31021 tttgcttgca taggaccgaa atcagctact gtgtataaaa aagtgtccag atttcttttc
    31081 gctgtgattt tagaatctaa ctgacgcaaa gaaccagctg cggcgttccg aggattcgca
    31141 aagatatctt ttccctctgc ttctctgtct tggttcaatt ggacaaatga ccgttttggc
    31201 atgaaacatt cgcctcttac ttcgatgttc attggttctt ttaatttgat tgggaccgac
    31261 cggacagttt ttaggttttc agtgatgttt tctcctacag ttccatcccc gcgagtcgca
    31321 cctctcacga aatttccatc ttcataacgc aaagaaacgg aaagtccatc tatttttagt
    31381 tcacagcaat agtcaactac acgtcccact gctttttgta cacgttgatc aaaagctatc
    31441 agctcttctt tgctgaaaac gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt
    31501 tcaaaacctt ctaatacttt tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa
    31561 tcaggatatt gttcctcgat tgctactaat tctgcgtatt ctttatcata aacatagtct
    31621 tccactgttg gtttatcttc tacgtagtat tcgcgagacc attggttcag tcttgtacga
    31681 agttcttctg ctcgtttagc agcttcgtca atcgtcattt catgttccat catttaccct
    31741 ccactactaa gctttttcaa ttggcgcaaa ggcagccaaa agacgtttta ctccttgact
    31801 tgggaaagcc acatctagtt ctagatcctt cgacgtcccg cttactcgga cgactgttcc
    31861 tagaccccat tttttgtgtt tgactttatc tccaggtttc cagctgttat tttcccctcc
    31921 agtagcactt ttagagggaa caaccgctgt ctctggctgt ttatagctat atgctgtttt
    31981 tttagcgaat acaggcgttt ttttaggcgt aggacgcatg ccttcgattt ccagtaactc
    32041 ctgatcgatt tcttctacaa aacgagaagg tcggttatat tgcgtccgtc catataatgt
    32101 tctggagaaa gcatttgtta aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc
    32161 gagacggcgt tcttcctcca gttcgctttc ttccatcaat gctcgagata aaggaaaaac
    32221 cccctcttct agaccaatca ggaatacaac tggaaattct aatcctttgg ctgcatgcaa
    32281 agtcatcaat gtcacttggg aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc
    32341 cagatcattt aagaagaccg ttagtttttc ttctggtgca tcagcgtctt cttcattttg
    32401 ctgttcgaat tgtttgtcga attcttgtgt gaccgttaaa aattcctcca agttttcgat
    32461 gcgtgcttgt gcttctaatg tattttggat ttttaagtct tctagatatc cgctacgatc
    32521 caaaacctct ttcgtcaatt ctgtcactgt caagtatggg atcatctgag tgacttcttg
    32581 gatcatctct ccaaatgcac ctaattgctg tcctgctttt ccaccgatat ttgccagatc
    32641 cacattttgc gctgcttcaa gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt
    32701 ttcgatagat cctggtccga tgcctcgttt gggcgaatta acgatacgct caaaactgat
    32761 tgaatcttga ggatttacca gcacattcaa atacgctaaa atatctttga tttctttacg
    32821 atcgtagaac ttgtgccctc caaccatttt ataaggaata tttgctttca acagagtttc
    32881 ttccattaca cgagattgtg cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg
    32941 atgattgctt cgaatctctt cttgcatccg gtctacgata aaacgggttt cgtctcgttc
    33001 attatctgca cggtagtaag tgatcttgtt tccttctttg ttttctgtcc acaagttttt
    33061 tggttttcga ttgctattgt tttcaatgac ttggtttgct gcgctaagga tgtttttcgt
    33121 ggagcgatag ttttgttcta gcaaaattac tgcagcatct ggataatctt tttcaaaatc
    33181 caagatgttc tgcatatctg caccgcgcca gccgtaaata ctttgatctg catctcccac
    33241 gacgcacagg ttcctaaatc tacctgccaa taaattgact agcgtatact gtgcgtgatt
    33301 tgtatcttgg tactcatcta catgaatata atggaatttg ttttgataat acgtgagtga
    33361 atcaggatgt tcttcaaata atcgaatcgt attcatgatc aggtcgtcaa agtccataca
    33421 ttgattatat tgaagctctt tttggtaagc agcataacat tttgccgcaa tctcttcgta
    33481 ataactgcct tgcatttttg caaaatcctg aggggtctgc aggctgtttt ttgcttgaga
    33541 gattgttcct aatatacttc gaggatcata ttttttcgga tcaatgttca gttcttttag
    33601 tatccgtttc atcaaggtca gctgttcaga agaatcgatg attgtaaagt tccggttata
    33661 cccgataaaa tcaacatctc ttcttaaaat acgcacacac atcgagtgaa aagtcgatac
    33721 ccagacatct tcgcctccag atgcgagaat cgcattgaca cgttctttca tttcttttgc
    33781 agccttattg gtaaaagtaa tcgctaggat gttccaagga ttgacttctt tttcctcaat
    33841 caaataggca atgcggtgtg tcagcactct cgtctttcca cttcctgcac ctgccattag
    33901 cagtaatggt ccgtcagtgt gaagaacagc ttctttctga cgggggttca tcccgtttaa
    33961 taattcagct ttggatgaca ttaacaacat cctttctttt atcaatcttg ttaattatag
    34021 cattttcttg gctttcatta aactagctag ttcaaaatca tttgtttctt tttatggatt
    34081 tttccttgtc aattcgttga tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga
    34141 aaaaaaagga aaaatcaaga aattagataa gactcagata tattttattc taaaaggaat
    34201 atttgttgga ctgctgacag gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct
    34261 aagcgattta gttcgaagta tctatgaaat gagtcgggaa cagccgattt acttaattgg
    34321 cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc
    34381 ggatatcaag ggaagcggga ttccacaagt agtgggacag cttcgcgggg aattatcaat
    34441 gaactggttc tctgttttat ggaaaaagtt cattggcggt gtactttcag ttggtgcggg
    34501 cctttttcta ggtagagaag gaccttctat acagcttggc gcatccattg gacaaggagc
    34561 tggacaattg tttcggagtc catcttcaga agaaaagatt ttaatttcta gcggtgcaag
    34621 tagcggactt gcagcagcat tcaatgctcc tatcgcagga ttactctttg ttttagaaga
    34681 aatccatcac tccttttctc cacttgtttg gctgacatca tttgcttcag caatcactgc
    34741 taacttcgtc tctctttact ttttcggctt gagacctgtt ttatatctag gtgaccttcc
    34801 ttctttgccg ttgaaatatt atggttcctt agtgatctta ggagtacttc taggcatctt
    34861 aggttttatt tatcagaatg tcttgctggc tttgccaaaa ttttacaacc gattgccttt
    34921 accagcttat tttcacgggt tcgttccttt cttgctgatt ttgccaattg gttatttatg
    34981 gcctagtact ctaggcggag gcaatcaaat cgttttagct tttggacatg cttccttgcc
    35041 aatcattatg attatcggtt tgtttgttct tcgattcgtt ttttccatga tttcttacgg
    35101 tgggggtctt ccagggggga ttttccttcc gatattgaca ttgggggctt taatcggtag
    35161 cttttatgga aacattatgg ttgatttcgg gatggatcca atctacatca aggattttgt
    35221 cgttattgcc atggctggtt atttcacagc gattggaaaa gcaccgctga cagcaattat
    35281 tttagtgaca gaaatggttg gctctcttaa tcatctgatg cctttaggat tggctgctct
    35341 tgttgcttat atcgtcaatg accttctcgg cggtaatcca atttacgaat cattattgga
    35401 acgtctactt ggaaatactc agaccgcctc actaaaaggt gcaaggacaa cttttgattt
    35461 tccagtaacg gcagagagtt ctttagacgg attaatggta cgagacttca attggcctaa
    35521 aaatatgctg ctaatctcta tacgcagagg aaatcaggaa atcttgactc acggtgatac
    35581 gatcatgaat gtaggagata ttttggttat cttgactgat gaagcaaact tgccaagaat
    35641 caagcaggaa atcgaggcta aatcttctat cacaaatttg aagcaatcat tttaacgaac
    35701 atggaaaaac tattttacaa caactattca tctacactac ttgattctat atgattctat
    35761 aaaaaaagaa gaggctggga aaacccagcc tcttcttttt tatttctatg cttctgctac
    35821 tgcaggagct tttctcaata aaccataaat cacaccagat acgactgctc cgatagcaat
    35881 aaacaccaaa tacatgaccg ggtgactcag taagaataca acgaaaatcc cgccgtgagg
    35941 agccagtaat ttgattccga aagctccgac taatccgcca gttaaagcag aaccaataat
    36001 aaaacttgga atggcacgaa tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac
    36061 aaacgatagt cccatcacga tgttcgtcaa accagcatct tgatctgttt tgctgaattt
    36121 attcttaaac aaacgtgtag caacgaaaat tgccaatgga ggtaccattc ctccagccat
    36181 aacagaagcc atcacgacac ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc
    36241 aaacacataa gcggctttgt tgattggacc gcctaaatct gccgccatca ttccagctaa
    36301 taatgcacca agtaaagcag cattcgaacc actcatactt gtcaagaaac cgttcaatgc
    36361 atcattgatt gctttcatag ggacattgac cagcaacatc aagaaaccag taatcagtaa
    36421 gccaaacact ggataaaaga ggatcgtttt aattccatcc aatgattttg gtaagttctt
    36481 gaataattta cgtaaaaata cgatgacata accagctaag aatccaccaa tcagtgcacc
    36541 taagaatcct gcaccgcctg tattagctaa tgcaccagca gcaaatccaa cgatcaaacc
    36601 agggcggtca ccgatacttg aagcaataaa tcctgctaat accggcagca tgaaaccaaa
    36661 tgcagcttgt ccgatttggt tgaaccagct agcagcttga ttgtaattcc ctagatttga
    36721 taattgatca tgtggcacgc caataaattg gtcgatcatg aaagaaagcg caatagcgat
    36781 tcctccacca ataacgaaag gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat
    36841 tctttgtccg atagtaccat ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc
    36901 atggaatact ggtgcagtac cactaagggc tagattgatc aactcttctg gtttacggat
    36961 accatcactg acaggacgat tgatcagttc ttttccgtca aagcgattca tctctacttt
    37021 tttgtctgct gcaacgatta caccttctgc acgggcgatg tcttcagcag tcaatcggtt
    37081 tttgattcct tctgaaccgt tcgtttcgac tttgatctcc acacccattt ctttcgcttt
    37141 tttcttcaaa gcatcttctg ccatgtaagt gtgagcgatc cctgttggac aagctgtaac
    37201 ggcaacaacg aattttcttt ctggatgagc agaagcttct gcagcttgtg tttcttgttc
    37261 tgctttttct tctgcttctt tttgctcttc agctgtttgg aacaagtctt ggacttcttc
    37321 tggtgtattt gcttctttta attttcctac gaaatctgca tcaatcagca atcgagatag
    37381 tgctgctaat gcttgcaaat gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa
    37441 cagataagtt ggctgtccgt ctaaggcttc ataatctact cctttttggc tttttgcgaa
    37501 taaaactgtt gcttctttaa ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc
    37561 aagacctgta gaagtttgtg cttcgcgggc tagaattcct tctttatatg tttcgatatc
    37621 ggaaatccgg ccggcatcaa acattttttg gaccatttca tcgattgcgc cttttttatc
    37681 agtcgcttgc aaatccatga tcatcgcttc tttgaccatt aaatccttga tattcatgat
    37741 tatcctcctt gaaactaatc aatttttgtg atttcaactt ctggcagcaa ctcatcaatc
    37801 aattcgcgag ctgccaagtc atcagaaaaa gctgttgcgc ttccgcaagc aactccccat
    37861 ttaaaggctt cgattggatc tttcgtacgg ctgtaagccc caataaagcc agcaatcatt
    37921 gaatcccctg cacctactga atttttcaat gggcgaacta atacattcgt tttataagtt
    37981 ccttgttccg tgaagagcaa agcaccgtct ccagccattg agatcagcac attctgtgcc
    38041 ccttcagcta gtaatttttt cccatacgga atgatttctt ctacagattg gaaggttgtg
    38101 ttgtaaagct cagctaattc gtgattgttt ggtttgatca acaacggttt cttaacaagt
    38161 gcatttttta ggtcatcacc tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg
    38221 atatcaatca gttcttgata aaatcctttt cgcaggctag ctggcgcgct tcctgacaat
    38281 acgacgatat ctccttgtcc tactgttcct aatgcttgct tcaactcatc gatttcttca
    38341 tctgaaatag atgggcctaa gccattgatc tcagtttcag tgtctgattt tagtttgata
    38401 ttgattcttg tatcttcttt tacggttgtg aacaaagact ggatttgttc tttttcaagc
    38461 cacttcgaaa taaattcgcc agtgaaccct cctaaaaacc ctagtgctgt agattgagaa
    38521 tcgatccgtt tcaaaatacg ggaaacattg atcccttttc ctccaggtaa cttgaagtct
    38581 tccttcattc ggttcaaatc gcctagtttt aacccatcta cgcgtacgat ataatcaatt
    38641 gatggattaa gtgttactgt gtaaatcatt gtattgcctc cttaattgtt gtcttttcac
    38701 ggaattcatt aacgaattct aatggacaac gctcggttat gatcgtggct gcttccaaag
    38761 gtgcaacaga ggtgaaggac aactggttga atttggtatg atcaatcaat acaaatgtct
    38821 cttcggaatt tttcatggct aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga
    38881 atccttgttc aagatgggca ccgttcattc ccataaatgc tttattaaaa tgaaaatttt
    38941 ttagttgttc tatcgcatta gatcctaaaa cagcattagt tgccagcttt atcgttccac
    39001 ctaaaataat ggtagcaagc tccaaatcaa ctaaatttgc cgcatgttga acggaatttg
    39061 tcactactgt gatctgtttt ccttgcagaa aaggaatcat ttctaacgta gtagagccag
    39121 cgtctaaata aatgacatct tcgtcattaa tggtttcagc tgccgatttg gcgatttgct
    39181 gtttttcgtg aacgtttttg agtgattttt cactcatatt ctgctcaaaa ttcaataatt
    39241 gctctttctt agctccgcca tgaactcttt ctagaaaccc actattctcc agctcttgta
    39301 aatcacgtct tatcgttgat tcagaagcat tcaaaaggtt tacaagatct tgtgatttca
    39361 cgattttctg ctgttcaagc aagtctagga ttttttgtct gcgttcctct gtaagcattt
    39421 tcacgatcac ctcaatacaa ataatagcat attttttttc tactagcaat ataaatcagt
    39481 caaaaacgtt cataaaaatt ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga
    39541 ctggtttttt atttttttgt ctatcatcaa gccgttttat cgttttttat tttcgcttct
    39601 tgtttttttc atataaacag aagcaggttt tctttcctta tgaagaagat aataataaag
    39661 tcccatcccg ccaaacatga ccaaaaaacc gatgactgat cgaataggaa aattaggaac
    39721 aaaaaagaga cagacggata ataatgatgc cggaaccatt attcgataat agagttgttt
    39781 atccattttc ttcacctcat attttttgta aagtaatcaa ttgttctaat tctcggtaat
    39841 ctaaaaattg cacacgcaga tgctgctcac ctttcttgac atcgaattgg atacttcgcc
    39901 gctttttctt tattcgtttg aaggtaacat cagagagtct atgtttgatt tcctttaacg
    39961 gtaattctga cagggattct gaaggaacat agattcttaa ggataaaaga tactcttgat
    40021 atttttcatc tgttacctct acttctagat attccctaat ataataacaa taagcttcga
    40081 ttccattatc caaagcaaca gtcggatcga taactttata gcacatcatg cgtgatcgat
    40141 tctcgtcaac ccaaggaaaa tacgcctgag catttagtgt tccgcgaaca cgcctttctt
    40201 tataatctcc ttgttggata tcataacttt ttgttacaaa aaatgaagca ttctttttag
    40261 gaacaggttg catttgtgca ttctgatact ggacagattg gatcgtatgg tcagtgtaaa
    40321 tgtattcaac gaatggcggt ttcaataaac cgacaccatt gtcccaagca agatgatcaa
    40381 acccaagctg gtccctaaaa taggattgaa acatttgatt gtttggcttt tgccagttaa
    40441 aggggtctcg ataaagctgg atacatatga cttcgttttc taattccttc actttttcta
    40501 cttgttggat gtcgagaaaa atcggtttag ggatgatttg atgataatag cgactataat
    40561 acatttccca acacgaagtg aaacataaac cttggtaaaa aagatcgtat cctggaaatt
    40621 ggttgcaatc gactactact tccccgtttt gttgtttcat tttaggtaaa tgttcgattt
    40681 catccttttt ctcaaacaat agccgttgag agatctgttt tgtattgtga tagataaact
    40741 cgctataagc tcgcatatag gcgaacaccc catgagcaat catttttttc tgaatgaagt
    40801 ctcgaatcaa aaattgcttt tctttgaaaa cagagataga caatacattt tttatcaata
    40861 attgattatc ttccaagcga aaactacatt cgttctcgtc atttcctaca actaaccaac
    40921 tttcgtctct taccgatgct agcattattt tttcttgaag ctttcgaaga tcatacggat
    40981 ccgcttgttg tgtgaaagag cggataaatg ttggctggat caacagacgt gtcaaaatag
    41041 cccaatcgct tatttcctgt tttttttctt cttcatttaa gtctgtgagc tggatagtca
    41101 tcaataattt atcttcgcca atcattttct cacctctttc aaatcttttt ataatttaat
    41161 tataaccaat gataaagaaa aactaaaact ttatgtctta gaaaccaatt tggacgatta
    41221 catgatagac taagtaaaaa gaaacaaaga ggtgtttaca atggggttga aatttgaacg
    41281 tacagataat ttagataaac ttttcgaatc atttgctgtc gatccggaaa aaacagttgc
    41341 accaaaagaa gagatcgatc gatttctaaa accacagcca caaaatgaaa agaaaaaaga
    41401 caagaaacaa aagtaagtta agctcaaaaa aagacttacc agccaaactt ttatgaaaga
    41461 gtttgactgg taagtctttt ttattctttt tatgattcaa ttgcttgttc gattgctgcc
    41521 cacgcttctt cttttccctg ttttgtaacg gaagaaaaga gaataaacgt gtcattgcga
    41581 tcaaaatcca acgctttttt gatttcgctc tcatgcttgt tccatttacc acgagggatc
    41641 ttatctgcct tagtggcaac aacaattaca gggatatcat aatacttcaa aaattgatac
    41701 atctggatat cttcttgaga aggcttatga cgcaaatcta ctaaagaaac gaccgcacgt
    41761 agctggtctc tagtcgttaa atatgtctcg atcatctttc cccattttgc tcgctcagtt
    41821 ttggatactt ttgcataccc atatcccgga acatcgacaa aatgtaatgc gttctcgatc
    41881 aaataaaaat tcaatgtctg ggtctttcct ggcttaccgg aagtacgggc taaattcttt
    41941 cgatcaatca atgtgttgat aaaagaagat ttccctacat ttgatcgtcc tgccagagct
    42001 atttctggca attgtgtatc tggatattgt tttggtgcca ctgcacttat gataatttca
    42061 gcttgatgga ctttcatggt ctctctcctt aacaaggtga tgaataaagc aagcggctgt
    42121 cgcaaaagcc aaaatgactt ttacggcagc ctcttttcat caacccgctt ttttctctga
    42181 atctttatag attactgttg gctcaccaga caattcagct gcttcttttg tgataatcac
    42241 tttttcgatg ttttcatttg aagggatatc aaacatcaca tccatcatga tttcttcaat
    42301 gatggaacga agtcctcgag caccggtatt gcgttcgatt gcttttttcg caatggctct
    42361 caacgcttca ggttcgaatt caagttcggt attatctaga gaaagaagtt tttgatattg
    42421 tttgactagc gcatttttcg gctcagtcaa gatccgtacc agatcatctg ttgtcaattt
    42481 ctctaaagct gccataacag gtaatcgtcc gataaactca gggatcaatc cgaattttag
    42541 taaatcttca ggaatgatat gctgcatgac actttcgtct tcagaaagtt tttggttttg
    42601 tgtaccaaat ccaatcgtct tttcgcccat acgattcttc acaatcgttt cgatgccatc
    42661 aaatgcacca cctacgatga acaatatgtt cgtcgtatca atctgaataa attcttgatg
    42721 tggatgttta cgtccgccct gaggtggcac acttgcaaca gttccttcta gaattttcag
    42781 caaggcttgt tggacacctt cgccggaaac gtcacgagta atagatacat tttcgctttt
    42841 acgcgcaatt ttatcgatct catcaatata gatgatccct ttttctgcaa gttccacatt
    42901 gtaatcagca gattgaagca atttaagcaa aatgttttct acatcttccc ccacataacc
    42961 agcttctgtc aaacttgtcg catctgcgat tgcaaaaggt acattcaatg tttttgctaa
    43021 tgtttgtgct aaaaatgttt taccagaccc tgtaggtccg atcaagcaga tattgctttt
    43081 ttgcagttca acatcatctg tgttttcttg tgcttgctga ttgacacgtt tatagtgatt
    43141 gtatacggca acagctaacg agcgttttgc gcgatcttgt ccaatcacgt agttattcaa
    43201 tacttcaaga atttctagtg gtttagggac ttcagtgaat tcgcgtacag cttcttcata
    43261 aaattcttca tcaatgattt ctttacatag gtcgatacat tcattacaga tgtagacccc
    43321 tggaccagct acgatctttt tcacttcttc ttgggttttc ccgcaaaaag aacagcgtac
    43381 ggtctcattg ctacttggat tgtcatacat ggtcttcacc ccttaacttg caaaaagtat
    43441 gcaagaaact atcaaagact ttacagtctt ttactatcat aacatagttt agtaaaaaga
    43501 aaaagtgttt aacaaaggat caactgacct atacctttgc attttttact cttaacaaaa
    43561 aataaggaac tgtgacaaaa tcgtgtcaca gtcccttatt tcgaataaac aattttctag
    43621 aagtagcttc ttcagaaatg atttattact tgatttctat tctttcgctt ctgtcgctgt
    43681 acctgtgatc aattcgattg cttttttcat tgtcacatca tgttccagca tatcttctgt
    43741 caacacgcgt ttgatttgtt cgattggcat gttgtatgtt tctgataatt cttggatttc
    43801 tgcattgatt tcttcttctg ttgcttgaag attttcagca gctgcaattg cttcgatcac
    43861 aagatttgtt cttgtgcgca tttctgcttc accttcgaat tgtttatgca agtcttcttc
    43921 tgtagaacca gttaattgat agtacatttc aggagagata ccttgacgtt gcatgttgtt
    43981 caagaattcg tccattgaac gatgtacttc atcatgaacc atcacgtgtg gcaattcaac
    44041 gatttctgcg ttttcaacag ccatacggat cgctgcttca tcttttgcat cgtccgctgc
    44101 tttttctttt gtttctacta attctttgcg atatttttct ttcaattcgt ctaatgttga
    44161 tacttcgtca tcaacatctt ttgcgaattc gtcatctaat tcaggcactt cttttgcttt
    44221 tacttcatgg attgtcactt tgaagactgc ttcttttcca gcaagatctt ctgcttgata
    44281 atcttctggg aaagttacat ttacatctag actttcacct gatttatgac caactaattg
    44341 atcttcaaaa cctggaatga atgagcctga acctaattca agtgagtaat tttcgccttt
    44401 tccgccatca aacgcttcgc catctacaaa gccttcgaag tcgattacta ctgtatcgcc
    44461 attttcagct gcagcatctt ctttgataac taattcagct tgtgcttctt gttcacgttt
    44521 gatacgtgca tccacgtcag catctgttac ttcacggtct tgtttttcaa caactagatc
    44581 tttatattgt cctaatttta cttctggttt cactgttact tcagcagtga ttacccagtc
    44641 ttgacctttt tccatgcttt caacatcgat ttttggttga gaaacaggat cgatgccagc
    44701 ttctttgaca gcagcttcat aagcttctgg taatacagca ttcaatgcat cttcatataa
    44761 agcttcttca ccgtacatac ggttgaatac ttgacgagaa actttccctt tacggaatcc
    44821 aggtacgttc aagctttttt tcactttatt aaacgcagtt gttaatcctt tttggatttc
    44881 gtcttgcgca atcgtaaatt ttaaaacacc atcattcgtg cctttttttt caaatgttgc
    44941 agtcatttaa ttccctccga gtttagaaat tcattttata catcaaaaac gtttcatgca
    45001 tacctttaaa tagtacacta accctttcaa attgtaaaca tattgaatgg atagaatcct
    45061 tgtttttcat aacattttta tctttttcac tgataaaatc taatggaaga agatcatttg
    45121 ctgcaaaatt ttttctcttt tttttaaaaa atcttgtttt tcatcttctg taccttcata
    45181 catcgttttt cctgtacacg cctctacagt cagttgaaac cagcgcttcg ggtcttcgat
    45241 gtatgtatca tagacaggat aaacaattgc agattgcaac gtgaattctt cttttaacac
    45301 ggaactaagc agcgcatctt cattcccgta acgatctgaa aatacacata attgctggta
    45361 acttgcctgc tgctgcggct ttggcagatc ttttggtatg actttgacta acttttcttc
    45421 aatcgtcaaa tagaaaactt gatggcattc gttcaattgc ctaagtgtct ccagtaattt
    45481 tgcccttgct aaaatatgga cctctggcga tacaataagt ttactcgctg tttggataaa
    45541 ctcatgataa ggaagctgtt cgatctcatc aatagacgca agctgacgtg caggttccaa
    45601 cgaaggaagt tcctccgatt tattgtggag tgccttgatc ttctgcctct ccatttgtcc
    45661 ggataattct tctgcaaatt ggattttttc aatgacccgc tgctcgaaag cttcataaaa
    45721 atctgtactt gctatcaatt ttctagcata taaaaaatct tgtgtactga ttaacaagtc
    45781 aaaataaaat tcagcaaact cctcattttg aagatactgt ttttcatgca agagtgcttg
    45841 cttcaatgct tcttttttct cgtccaactc gtaaagacaa aaaacaagca ggctattagc
    45901 ctcaaagctt tctttcaact cgtaagctcg ttgaaataat tcttttgcct gctgatattg
    45961 ctggtttgct agtgcttgtt gtgccactcg cattaaattc tcatattttt ctggaaaatc
    46021 aatcggcttc acgcagtgtc ctcctcttgc tcatattcag ctcatgttca atagtagcaa
    46081 aacaaaaaaa gcatgacaat taaaaataac tgaagcaccc tctaaaagct agattttaga
    46141 tctgactttt agaaggtgct ttcaactgtt actgcttttt attgctcatc agctgttgac
    46201 ttcctggttg aacgctttga tcagattgat cgtaacaaca cttgtattag tgaaaaaatc
    46261 gcccagatgt tgctttttct gattaaagac tgctggaaga taccctaaca tcagcaaagg
    46321 aaattttaga atgaaccggc aagcaccttc tctaactaaa acggttgacc agctaagttc
    46381 ttcttcatca aagctgatga ctcgaatacc aaagatcatt ttcccaatcg tttggccatg
    46441 attgaatttc gtcaataaaa taaaatacgc tagataaata agtaaggctg tgaatccgta
    46501 gagactcaga taagaatctg aaaaattaag ccaacctaga tttttggcaa taccaagtgt
    46561 taaacgcgta atagctccga tacacaaaag atcgaccaag aaggcaaaca ttcggataaa
    46621 aaaacctgca tagaaatact taggaaaatc atttgctttt ggcggttcat ttcttgtgtc
    46681 cgtatacttt ttccatttac gttgtttctt tttgatttct tcgggcgtca aaggctctct
    46741 ttcaaaagag ctcagtacct tttgggaaaa gttggagtca ggtcctacag caggctgctg
    46801 ttgcttatct ttttcttgtt ccttattttc atttgaaagc tctgacatgc ttactcacct
    46861 ccgtaataat acattgcttt tggtgattcc gctgtcccta gattctcaag aacggataag
    46921 atctgactag tctcagaagg cttcaatccc tggaattctg ctagtttact tcctaaccat
    46981 gtactagcaa acccagatgg cgtggaagaa tattctacta gttccgcatc ttcaagctgt
    47041 tgttcctttc gtatagcttt taaagcatct tcaggaaatc ccaattcatc gactaaacct
    47101 acttcttttg cttggacacc atcataaatc cttccatcag caattttttt gacttcttct
    47161 tcagatttat tccgtccctc gctgacgatt gctacaaaac gactatatgc gctgtcgata
    47221 tatgcttgaa gcaccgcttc ttcttctttt gtctctggtc gcgttcctga acccatatct
    47281 ttcaacgctc cgcttttaac tgttgtatct tccacaccca gtttttctag aagtcctgaa
    47341 taattcaatc ctgacatgat cacaccaatt gagccggtca ctgtttcttc tgttgcaaag
    47401 attttatctg cttgagcaga aatatagtag ccaccgctag ctgccatgtt tttcatgctg
    47461 acatacattg ggatatcatg ttctttacga atcgtatcca atttcttagc gatttccgca
    47521 ctttcgtaga caccgccacc aggagaattt acttctaaaa agataccttt gactgttgga
    47581 tcttcttcaa tcgccttcag ttgttccata aaatttttat gattgtaacc ttcagaagca
    47641 aataaacctg aagaccctcc cgcagcaatc gttccatcta ctgataattt tacgattttt
    47701 tgcgtactgt ctccctcttc caacacattt ggagctagtt cttcatcccc atataaccaa
    47761 ctgttcaatc cctcaatttg atcgttttcc tgaggcttag tcaaactagc tgaaactaac
    47821 gaaaccacaa ataatcctgc agcaatcaga actgctgccc aacgtctttt attcattctt
    47881 tctcctttct aatacaagtt ttttatgacg atcacagttt ctgactgctc agaagtgtcg
    47941 aaccgctctg ctttttcttc ccgcgcatac ccggaaaacg actcttttgt taattcatct
    48001 tctaccgtaa actcacgagc ttgagcaact tccaaggcat caggtgcgat ttcttggatg
    48061 ctatcccagc cagtatctaa aaatattttc gtatcatctg atacaccagc tgatgcttcg
    48121 aatttggaca attgtttttt taacattccc agtgtcatga tctcaatttt tgaatccatt
    48181 tttactcctc ctctatccga ttactttcta ttgtaccgaa ctcctgaaaa agatgataga
    48241 taaaaaccca ttattttttg acataaaaag gacatttgac aaagaatttg atatatacca
    48301 atttattttt tattcttttt ttgttgacaa gaacattcgc aaaaggttat gattttaaat
    48361 gtttccagat aaaaaacaaa aaggaggata gttatacctt ttaggatagc gccaggtctg
    48421 tgtaaacaga tgacgaggag agggtttatc gaaagattcg gcggatggcc ctagggactg
    48481 cactc
//
LOCUS       DACCVU010000008        46228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCVU010000008 DACCVU010000000
VERSION     DACCVU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.008"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            200..1081
                     /locus_tag="IVW29_000385"
     CDS             200..1081
                     /locus_tag="IVW29_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7837893.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1094..2704
                     /locus_tag="IVW29_000386"
     CDS             1094..2704
                     /locus_tag="IVW29_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7837894.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2781..3440)
                     /locus_tag="IVW29_000387"
     CDS             complement(2781..3440)
                     /locus_tag="IVW29_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7837895.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3723..4388)
                     /locus_tag="IVW29_000388"
     CDS             complement(3723..4388)
                     /locus_tag="IVW29_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837896.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4535..5140)
                     /gene="dhaL"
                     /locus_tag="IVW29_000389"
     CDS             complement(4535..5140)
                     /gene="dhaL"
                     /locus_tag="IVW29_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7837897.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5168..6151)
                     /gene="dhaK"
                     /locus_tag="IVW29_000390"
     CDS             complement(5168..6151)
                     /gene="dhaK"
                     /locus_tag="IVW29_000390"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7837898.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6153..6548)
                     /gene="dhaM"
                     /locus_tag="IVW29_000391"
     CDS             complement(6153..6548)
                     /gene="dhaM"
                     /locus_tag="IVW29_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7837899.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6702..7022
                     /locus_tag="IVW29_000392"
     CDS             6702..7022
                     /locus_tag="IVW29_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7837900.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7134..8003)
                     /locus_tag="IVW29_000393"
     CDS             complement(7134..8003)
                     /locus_tag="IVW29_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7837901.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8099..9403)
                     /locus_tag="IVW29_000394"
     CDS             complement(8099..9403)
                     /locus_tag="IVW29_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7837902.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9400..9909)
                     /locus_tag="IVW29_000395"
     CDS             complement(9400..9909)
                     /locus_tag="IVW29_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837903.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10084..12219)
                     /locus_tag="IVW29_000396"
     CDS             complement(10084..12219)
                     /locus_tag="IVW29_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7837904.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYLKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12302..13957)
                     /locus_tag="IVW29_000397"
     CDS             complement(12302..13957)
                     /locus_tag="IVW29_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7837905.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13959..14273)
                     /locus_tag="IVW29_000398"
     CDS             complement(13959..14273)
                     /locus_tag="IVW29_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7837906.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14443..14703
                     /locus_tag="IVW29_000399"
     CDS             14443..14703
                     /locus_tag="IVW29_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7837907.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14717..15031
                     /locus_tag="IVW29_000400"
     CDS             14717..15031
                     /locus_tag="IVW29_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7837908.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15761..16315)
                     /locus_tag="IVW29_000401"
     CDS             complement(15761..16315)
                     /locus_tag="IVW29_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7837909.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16441..18370)
                     /locus_tag="IVW29_000402"
                     /pseudo
     CDS             complement(16441..18370)
                     /locus_tag="IVW29_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18676..19392)
                     /locus_tag="IVW29_000403"
     CDS             complement(18676..19392)
                     /locus_tag="IVW29_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7837910.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19393..21216)
                     /gene="glpO"
                     /locus_tag="IVW29_000404"
     CDS             complement(19393..21216)
                     /gene="glpO"
                     /locus_tag="IVW29_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7837911.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21230..22726)
                     /gene="glpK"
                     /locus_tag="IVW29_000405"
     CDS             complement(21230..22726)
                     /gene="glpK"
                     /locus_tag="IVW29_000405"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7837912.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22862..24322)
                     /locus_tag="IVW29_000406"
     CDS             complement(22862..24322)
                     /locus_tag="IVW29_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7837913.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24312..25607)
                     /locus_tag="IVW29_000407"
     CDS             complement(24312..25607)
                     /locus_tag="IVW29_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7837914.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25939..26616)
                     /locus_tag="IVW29_000408"
     CDS             complement(25939..26616)
                     /locus_tag="IVW29_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7837915.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26646..27737)
                     /locus_tag="IVW29_000409"
     CDS             complement(26646..27737)
                     /locus_tag="IVW29_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7837916.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27838..28272)
                     /locus_tag="IVW29_000410"
     CDS             complement(27838..28272)
                     /locus_tag="IVW29_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7837917.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28856..30313)
                     /gene="ascB"
                     /locus_tag="IVW29_000411"
     CDS             complement(28856..30313)
                     /gene="ascB"
                     /locus_tag="IVW29_000411"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7837918.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30310..32205)
                     /locus_tag="IVW29_000412"
     CDS             complement(30310..32205)
                     /locus_tag="IVW29_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7837919.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32263..34038)
                     /locus_tag="IVW29_000413"
     CDS             complement(32263..34038)
                     /locus_tag="IVW29_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7837920.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34088..35584)
                     /locus_tag="IVW29_000414"
     CDS             complement(34088..35584)
                     /locus_tag="IVW29_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7837921.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35778..37400)
                     /locus_tag="IVW29_000415"
     CDS             complement(35778..37400)
                     /locus_tag="IVW29_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7837922.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37728..39041)
                     /locus_tag="IVW29_000416"
     CDS             complement(37728..39041)
                     /locus_tag="IVW29_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7837923.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39286..39510)
                     /locus_tag="IVW29_000417"
     CDS             complement(39286..39510)
                     /locus_tag="IVW29_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837924.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39530..39898)
                     /locus_tag="IVW29_000418"
     CDS             complement(39530..39898)
                     /locus_tag="IVW29_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7837925.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(40094..40804)
                     /locus_tag="IVW29_000419"
     CDS             complement(40094..40804)
                     /locus_tag="IVW29_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837926.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40822..41319)
                     /locus_tag="IVW29_000420"
     CDS             complement(40822..41319)
                     /locus_tag="IVW29_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7837927.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41356..41808)
                     /locus_tag="IVW29_000421"
     CDS             complement(41356..41808)
                     /locus_tag="IVW29_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7837928.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41943..42548)
                     /locus_tag="IVW29_000422"
     CDS             complement(41943..42548)
                     /locus_tag="IVW29_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7837929.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42998..43618)
                     /locus_tag="IVW29_000423"
     CDS             complement(42998..43618)
                     /locus_tag="IVW29_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7837930.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43620..44489)
                     /locus_tag="IVW29_000424"
     CDS             complement(43620..44489)
                     /locus_tag="IVW29_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7837931.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44512..44919)
                     /locus_tag="IVW29_000425"
     CDS             complement(44512..44919)
                     /locus_tag="IVW29_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7837932.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44938..46227)
                     /locus_tag="IVW29_000426"
     CDS             complement(44938..46227)
                     /locus_tag="IVW29_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7837933.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12993 a         9852 c         7908 g        15475 t
ORIGIN      
        1 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
       61 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
      121 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
      181 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
      241 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
      301 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
      361 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
      421 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
      481 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
      541 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
      601 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
      661 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
      721 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
      781 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
      841 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
      901 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
      961 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
     1021 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
     1081 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
     1141 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
     1201 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
     1261 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
     1321 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
     1381 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
     1441 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
     1501 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
     1561 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
     1621 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
     1681 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
     1741 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
     1801 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
     1861 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
     1921 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
     1981 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
     2041 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
     2101 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
     2161 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
     2221 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
     2281 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
     2341 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
     2401 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
     2461 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
     2521 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
     2581 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
     2641 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
     2701 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
     2761 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
     2821 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
     2881 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
     2941 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
     3001 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
     3061 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
     3121 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
     3181 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
     3241 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
     3301 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
     3361 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
     3421 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
     3481 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
     3541 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
     3601 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
     3661 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
     3721 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
     3781 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
     3841 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
     3901 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
     3961 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
     4021 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
     4081 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
     4141 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
     4201 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
     4261 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
     4321 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
     4381 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
     4441 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
     4501 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
     4561 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
     4621 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
     4681 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
     4741 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
     4801 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
     4861 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
     4921 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
     4981 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
     5041 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
     5101 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
     5161 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
     5221 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
     5281 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
     5341 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
     5401 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
     5461 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
     5521 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
     5581 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
     5641 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
     5701 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
     5761 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
     5821 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatcggctt
     5881 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
     5941 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
     6001 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
     6061 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
     6121 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
     6181 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
     6241 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
     6301 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
     6361 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
     6421 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
     6481 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
     6541 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
     6601 aatgaacaat tcaagtatat aataaatttc taattttggg aaaatatgtg tagtgcttct
     6661 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
     6721 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
     6781 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
     6841 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
     6901 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
     6961 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
     7021 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
     7081 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
     7141 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
     7201 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
     7261 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
     7321 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
     7381 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
     7441 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
     7501 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
     7561 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
     7621 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
     7681 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
     7741 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
     7801 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
     7861 ccagaagagc ttatggttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
     7921 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
     7981 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
     8041 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
     8101 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
     8161 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
     8221 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
     8281 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
     8341 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
     8401 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
     8461 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
     8521 gccagacttc tgttccacct tttgcccaac gttttctgtg tcgatatagc atcttcaacg
     8581 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
     8641 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
     8701 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
     8761 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
     8821 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
     8881 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
     8941 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
     9001 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
     9061 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
     9121 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tattcgatca
     9181 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
     9241 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
     9301 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaaatacata atagacaaac
     9361 aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt
     9421 ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata
     9481 aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag
     9541 taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt
     9601 gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa
     9661 aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta
     9721 caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg
     9781 aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta
     9841 attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac
     9901 ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc
     9961 atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc
    10021 atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct
    10081 taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta
    10141 catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg
    10201 atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata
    10261 tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac
    10321 tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc
    10381 agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc
    10441 gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg
    10501 gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat
    10561 aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt
    10621 aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg
    10681 tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg
    10741 acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc
    10801 tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat
    10861 ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc
    10921 tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc
    10981 ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg
    11041 attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg
    11101 atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc
    11161 atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt
    11221 tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc
    11281 cgtgatacgt ttgagataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac
    11341 cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga
    11401 aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt
    11461 atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact
    11521 tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg
    11581 ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac
    11641 ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc
    11701 tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac
    11761 cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca
    11821 atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga
    11881 ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa
    11941 taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc
    12001 gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc
    12061 aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc
    12121 cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt
    12181 cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta
    12241 aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt
    12301 atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac
    12361 catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac
    12421 gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt
    12481 cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt
    12541 ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc
    12601 attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta
    12661 ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga
    12721 agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt
    12781 ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca
    12841 gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga
    12901 cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag
    12961 tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc
    13021 cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa
    13081 gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat
    13141 caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga
    13201 ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat
    13261 cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag
    13321 cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag
    13381 ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt
    13441 ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga
    13501 tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat
    13561 ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt
    13621 ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta
    13681 cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat
    13741 ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac
    13801 tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt
    13861 tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca
    13921 ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga
    13981 tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat
    14041 tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct
    14101 ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc
    14161 ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt
    14221 tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc
    14281 acctcattta catttttata aatacctcat ggggtatatt aaacaggagc tttattaata
    14341 tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat aaaataccga
    14401 taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa
    14461 aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc
    14521 gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt
    14581 gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat
    14641 agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag
    14701 taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga
    14761 aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc
    14821 tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt
    14881 gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag
    14941 ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca
    15001 atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag
    15061 cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag
    15121 gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    15181 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    15241 agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc
    15301 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag
    15361 gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga
    15421 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    15481 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat
    15541 agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    15601 ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac
    15661 aacttctcct gtttttggtt cggttggctt attatcgaga agtagctact gggagtctat
    15721 ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct
    15781 ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa
    15841 aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa
    15901 catagatgac agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt
    15961 tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa
    16021 acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca
    16081 atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag
    16141 caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat
    16201 aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg
    16261 ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat
    16321 cgccctacct cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa
    16381 tgatttcact gtttttattt ttctttacag acactgatat tatgagctaa tttgagataa
    16441 tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac
    16501 tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt
    16561 ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg
    16621 caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat
    16681 cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat
    16741 ccgatcacct tttgcaaaga gctgacgttc ttttttattc tccttcctat ttgtaactgc
    16801 tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc
    16861 tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct
    16921 ttctcctgtt tcttccaacc cgcaactgat atttaggacg aagtgcttcc cgatcgaaag
    16981 ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa tttcttgatt tctccataat
    17041 gggtcgtggc aatgatcaat gccccttgct catacataga ttccatgatt gcgatcgcta
    17101 gtgcagcccc ttcatttggc tctgttccac tgccaatctc atctaaaagt accaacgtat
    17161 tcctttttat ctttttcaaa atagcagcga tgttttgcat atgaccagaa aatgtactca
    17221 acgcattttc taaattttgg tggtcaccga catcgacgaa aatctcatca aagacagcaa
    17281 gctctgtccc ttcttttgcc ggaacttgca taccgaacat cgccatcaaa gtcaataaac
    17341 cgactgtctt caacacgact gttttgcctc cggcatttgc tccggtgata atcaagcctc
    17401 gatagtcttt accgatttca acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc
    17461 tgccttgctt gataatgata tgttccgatt tattgattct aggtgtgctt ccattgattt
    17521 ctcggctaaa ttttgcccga gcaaagatga tatctaacac cgtgatcgtt tcgatcaata
    17581 aatcgatcac ttcttcgttt tctgcgatag caccagtcaa tgccgccagg acttgatatt
    17641 cttcactgat ttcttcagct tttaacaact gatagtgttc attcaattta gaaatcacag
    17701 ttggttcaat aaaaaccgtc gttcttttat ttgattcatc gataatcgtt ccatctattt
    17761 tatttttata gctggctttg ataggaatcg tatagtattc gcctttttga acaatcattg
    17821 cttcttggat catttctttg tttttcggat gacgcaagaa tttcagtaat tttgactgga
    17881 tttctttttc gattagttgt aattgtttgc gtactttacg caaattacgt gacgcgtcgt
    17941 cactgacttt attatttttt attttctggt caatcaactc ttctacattt atcaaatcag
    18001 gaagatgttt gctataagca aacaacaagg gtgtctggta ttgattctta tcaaaaaact
    18061 ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc gatcaaatcg gttggctgca
    18121 gaatcaaccc ttttttgact tgttctgtca gtgcatcgat cctcggcaac cccataaatg
    18181 gaacatgctg actgctttct aagatcaagc gtgcttctct tgtttcttct tgacgatcca
    18241 agacagtttg taggtttgtc gagaccgtca tatcgctgat tcgttttttg ctatagtttc
    18301 caattgctct tgcttggatt tcctttttga tccgatcaaa ttgtaattga ctaatcattt
    18361 cgttgtccat attctctcct tttcttgaag agatacctgt cccaaaataa ataagggcct
    18421 agagataaat aaaaggatag aacacagctt aaaaaagtca tgtcctatcc ttattagaat
    18481 acaaagatat tgttttcctg acaggtaccg tttcataagc acaccaaata aactttgttt
    18541 attggctatt cgacttactg attagatacc agctacactc acaaaacata ccccattctt
    18601 ctaggtctta tttattttcc acttaactat agcaagacac attttggttg tcaaacttta
    18661 gtatgtttta tagatttaca ccattaatga atagacacct accgcgataa ttgctccgat
    18721 cattggacct gcgattggta cccatgaata tgcccaatct gaagttcctt tattagctat
    18781 tggcaatacc gcatgtgcta atcgaggacc aaagtcccga gcagggttga ttgcatatcc
    18841 tgttggccct cctaatgaaa gaccgatggc taagatcaat ccacctacag cgaatacgtt
    18901 cgttccaggt gcaaattcgt tttgtccaaa agctagcaac ccaagaacta atacaaatgt
    18961 accaattaat tctgtgatca catttgctgg ataattccgt atagcaggac ctgttgcaaa
    19021 cgttcctaaa atcgcgcttt catctttcgt tgcattccaa tgtggcaaat aagaaagcca
    19081 aacaagaatg gcccctgcaa aagctcctaa aacttgcgca atgatgaaag gaacgacaag
    19141 attccaacta attgcccctg tcatcgccat agctagagag actgcaggat tcaaatgagc
    19201 tgggcccatg aatccagaaa tatagacagc cattgttaca gcgagtcccc aaccaaatgc
    19261 gatgacaatc cagccagaag cctgtgcttt acttttgttt aaattcacag ccgcacacac
    19321 accatctcca agcaaaacta ggatcagtgt cccaataaac tcccccatga tttgagtcat
    19381 atcactgttc atttatttct ccccctttag ttcccgcaga tctgattctg caatcaatgc
    19441 ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc cattcaaaat atccagcaat
    19501 tgcatcgatc acaggttgct tgatctcatc aagctgatct cgttcaaaca acagatgatt
    19561 tgtacgacga atgagataat cgcctggggc caaaaccatt tcttcttcta aaccataacg
    19621 taaccgagct gactcagcga gactcaaacc aggataaggt gccatttctt ttgctaattc
    19681 aaagatacgt cgggcattag tcccgtaaaa atcagcgata taagtagcat cttcttcctc
    19741 taaaccggct gctactcctt cttttgctaa ttctgtcact gtttcttcta atttagttgg
    19801 atcgaagttt ccacctgaaa tctgatattt tttagagtcg atttctttcg tctctattcc
    19861 gtattcttct tttaataatt ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta
    19921 atcggtgatt ttcccaccag ataaagtcag caaaccatct ggttctcttt ctaaagaact
    19981 acctctggaa atcgtagaag gtgctttttc atcacggctg ttttccaaat ggttcaacac
    20041 gtcttctact tcagcacgag aaactttgtt ttccttatat tcacttactg tatcgacaac
    20101 tttattgaag ctcttgtctg aaatcgatcc attatctcca ccattgtaat cagaaccaga
    20161 attaccaatc aacagtggac gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat
    20221 atttgcttct ggatagcgat gattgatcac atccaataga taatctacat cttcttgtgt
    20281 gactttcgga tccgtaaaat ctccttggta atccgtatct gtcgtaccaa agtaagtctt
    20341 gttttctctt ggaatagcaa aaaccatccg cccatcttgc tttcctgtat cgaagtatgt
    20401 gggttgcggt acaggcagtt ttttcgcatc tacaactaaa tggatccctt tagttggacg
    20461 cattttagga gagactggac gcgtaaaatt caagtttcgt actttatcta cccaaggacc
    20521 actcgtattg atcactaatt ttgctttgat ttcgatcact tcatctgtca gcaggtcacg
    20581 agctttgacg ccaacgattt gatcgccatc atataaaaag ccaaccgctt tcattttact
    20641 tactagataa gccccatctt cggcagcctt tttgatatta tcaatcacta aacgggcatc
    20701 attgttgcgg aaatctagat agacacccgc gccttttagc ccttcttttt tcaaaaacgg
    20761 ttctctttcc aatacttctt ctggtgtcag cgtataattc tcatatttgg ttcctgtcac
    20821 atttgccagt ttgtcataaa ggtccattgc tactttgacc gagaacatat tgaaggttgt
    20881 tgctccttcg ccctcataga ttggcaaaag cattggatct ggttttggga tgtgtggggc
    20941 aatactttgt acgactgcac gttcaccaac tgtgtcagct actacttcca catcaaatgt
    21001 cttcagataa cgaataccgc catgaacaag tttggtcgag cgagaggaag tcccttctgc
    21061 aaaatcctgc atttcgatca atcctgtttt gattcctgat gctgctgact ggactgccac
    21121 accggcacca gtgattccgc caccgatgat caacagatcc aattcttctt gctgcatttt
    21181 ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg atttcctcct tattcaaatg
    21241 cttgggtagc ttttactgct tttttccaac cgttgtaaag tttttctcgg cgctcttcct
    21301 ccatttgaac ttcaaatagc ttgccagctt cataaaattc gcggatctcg tctgtatctt
    21361 tccaataacc gacagctaat cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt
    21421 tttctgctcg ttggatcggt acattcaaga tatctgtttg gaattgcatc agatattcat
    21481 tgtttgctgc accgccatct actttcaata caggaatctt gattccagtg tcatcttgca
    21541 tcgtatcgat gatatctctc acttgataag caatcgactg taaagtagct ttaatgatat
    21601 cttcttttgt cgtaccacgt gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat
    21661 aaggagcacc tagcccaacg aatgcaggaa caacataaac ctcatcttcg cttgtagagc
    21721 gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat ttgaagtcca tctctcagcc
    21781 attgaatcga agaaccagcc acaaaaatac ttccttctaa agcatagtaa acctttccat
    21841 ttatgccgta accaattgtc gtcaataaat tatttttgga taattgcggt ttttcaccgg
    21901 tattcatgac aataaatgaa cctgtaccat aagtattctt gaccatgcct ggttcgaatg
    21961 ccatttgtcc gaataatgcg gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc
    22021 ttccataaaa atgataacct tgcgtataac cataaacttc tgagttagaa gtgacttttg
    22081 gtaacatcac tcgaggaata tttagtaaat ctaaaatctc ctgatcccaa tctagctgat
    22141 ggatattgaa caacatcgtc cgactggcat tcgagtaatc agtaacatgt gcttgtccat
    22201 cggtcagttt ccatactaac caagaatcga tcgttccaaa catcaattcg cctttttcag
    22261 ctcgttcttg cgcgccttct acatgatcaa gaatccagcg tatcttcgtt gctgagaaat
    22321 aggcatcgat cactaaccct gttttttcgt ggaaaaattc ttgatagcct tcatttttta
    22381 agttatctgc aattccagct gactgtctgg attgccaaac aactgcatga tagattggac
    22441 gtcctgtatc tttttcccat attactgttg tttcacgctg gttcgttatg ccaatcccag
    22501 caatctgatt tggtttgatc cctgattcga tgaacgctcc ggcaataact gactgtacag
    22561 aattccagat ttcatttgca tcatgttcca cccaactttc ttgcgggaaa tattgcgtga
    22621 actctttttg agaactgccg atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc
    22681 tagttgtacc ttggtcaatc gccatgatat acgttgattc tgtcattgtg attccctcca
    22741 ttatatgcaa acgctttctt taactactct tatcatacta ctgttttaaa aaaagaaact
    22801 gtcaaaattt tattcttttt ttcttttttt gacagttttt tattatgaag ctatttttta
    22861 atcaattctt ttcttttttc agcgtctcta ttcttctttt caatcgaata atgtcgtaag
    22921 gtgacgcaaa ttctgacaga taatattcac actgtttttt gtttctctgg caaaatgtga
    22981 tcaccaaatc atattcttta ccttcttcat aacttccaat ctcaataccg ccgattcccc
    23041 tcagttctct gatcagatat tgttgaaaag caatacgata aatcggcaga ctttctaaat
    23101 cgattccaat cactactggt tttgcaatat aaaaatcgat catcaaaaga acattggcat
    23161 actggatcat caggtaacta agctcactat cttcagctct tttcctcggc aattgctgca
    23221 ccgctgtttc ctgtaatttc gccagtaact gtgttaagga atgcccgagc attttctgct
    23281 gctgttccaa aagccgatca cgctcataga cttcgatctt tccaacaaag aaaaaccatt
    23341 cattgttgat ctgggaaatt tggtagccta ctgccttttc ttctttgatt ttcaaacgac
    23401 gaggacgata atgccataac atcgtttccc gaatgtaagt atctaaaaca gcggttggta
    23461 actttttgct ccgtgtcaaa tcatactgat aaaatatttc ttcgtctact atcgaaaaag
    23521 aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt cagttctgct gctgttcgac
    23581 taagatgcat cacgatgatt ggatcgattg cttgataaag ccggtccgac tgatagagta
    23641 atttcttttc tttcaaagtt gcgtacgtac ttttttcatt caatagtctt tttttcgtaa
    23701 tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac
    23761 ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct
    23821 gatcttccgg gatatattgg aatagttcat aataaaaata tcgtatctgt agctcttctc
    23881 cgtataattg accgtttttg atttgtaatc caaattcctc taagagctgg ttcaattctt
    23941 tgatctttcg aaaaacagaa gactcactga tattaaacgc tgtcgctaat cgaacgatgg
    24001 atacttgctg atgttctaat aaatagacga gcatcttaaa cttcaagctt tcctgcaata
    24061 aaaacaatag aattttttcc aaacttacat cttcgtccaa cacaagcagc gcctgatact
    24121 catttcgctg tacttctact tttttttcca tcattcggcc aatcaattca atatcttcca
    24181 aatattgatc aaacgaggct tttgacagtt ggacatgctc tctcaaacca ttgatagagg
    24241 ctgttccccc ttgcagaaca aggtttcgca aaatcaggat ctgtaactgc tcttttcttt
    24301 ctaataatgc ttcaatcttc ataggcagta ttcgccacct ttgaccacac catatctggc
    24361 acacggtatt ctggctggaa aaatagctca tgttcttcta actgttcaaa cgtccatcct
    24421 tcttctactg caattgtcag ttggtcaaga aggtggaaca tctctttttt gctttggagc
    24481 tgtgctccaa gaatcttttt ttcagaatca aatagaaatt tgagtttaac tttgctttga
    24541 tttattgctg taagcttgta gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg
    24601 ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc caaaccactg attgccgact
    24661 ggacgatagg ttccctgatc ctttgtctgt gagccactaa ttgtcttgct gacatttcgt
    24721 gctgtacgga cagcgttatt tactagagaa acatacattt tttcatttgt tggacgaaaa
    24781 gttacttgga tcgcatcgcc aatcgcataa acgtcttttt gagacgtttg caagtagtca
    24841 tctacccaga tcgttccatc atcattcaat gttaattgtt cttcccacat ctgattgtcg
    24901 ggacgagaat gattagccag caaaacaaga tcatagttct cctttctttt ttccgtctct
    24961 acaatagcgg tttcattttc ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc
    25021 ccttgatttt tcatttcctt tactaacggt tctgtcattt caggatctag ataacgaaaa
    25081 agaattgact ttcggctttc gtaaagatgg atttgttttt ttcttgatgc caacccttca
    25141 gctatttcga ttcctgcttg tcctgcacca ataattgcaa ttttctttgc attcatgatc
    25201 ttttgctgaa gagccgttac gttttgtatc tttttaactg ggtgaataag cgttgattct
    25261 gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc
    25321 aatttttttc ctcctgctaa aacgactttt ttttctttca atcctattac tgtcttttct
    25381 gtgtaaacat ggattcgata gcggtcagtc agttcttctt tcgtgatcca agaaagttgt
    25441 ttttcactgg taacctttcc ttttaatatc aaattgaacc cgctgggaat ccatcctaac
    25501 ttcgattgct tttcaatcaa atatatttca cttgttggca tcaattggcg aagataaata
    25561 gccgcatgaa tccctgcaaa cgacccgccg acaattacaa tgttcatctg cattcctctt
    25621 tcttataagg tgttctactg ctaacttctc aatcagaaaa cacagtattc ataactcgct
    25681 ttcaacctta tgatacatag cgatgcagca gaatcaaccc tctccatcat accgtgttct
    25741 aggtctgctt ctcaataatg tactagtgga cctaattgaa gagtatcatt tctgaagaga
    25801 tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc cttttacgta cctctgtcca
    25861 aataggaaac agaaaagaac gaaaagacga gttttagcgt gtgtaatgta atagttagaa
    25921 tagacacatc atttattttt aattctttaa tgcttcaaca actgcttcgc ctaattgatg
    25981 agcagattga ggattttgcc ctgtaagaag ccgcccatca atttgaacca cttctgaaaa
    26041 agctgctcca gactgatagt ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg
    26101 tacttcattc gttgtaccat ttgcttcctc ttcttcatta ctgaacccag tcactgtacg
    26161 tccagctaaa atagattttc cagactcatc tgtaactggc aacaatccta caacaccgtg
    26221 acaaacagct gagaccagcc cttcatttcg atagatttgt gaagcgactt tcccaagatc
    26281 tattgcttct ggaaaatccc acattgttcc gtggccacca gcataataaa ccgctgcata
    26341 atcatttgca tccacatcat caggacgtaa ggtatttccc aaggcccgat ttctgaaatc
    26401 ttcatcttca taataggccc aatcgatttc atccatattc aaaacacttt gcggttctaa
    26461 aggtacatat ccgcctttag gactgacaaa atctgcatcg atcccatttt ttactaatac
    26521 atcataaaaa tgagtcagtt cacttaacca caaacctgta ggacgctcga tcttttcgta
    26581 cttttctaca tttgttaata cgatcaatac tttcattttc ccatcctctt tctataaaaa
    26641 ttgcgttatt gataacgtgc tttcaagaaa ttatataatg ggcttggcaa ataaagctct
    26701 ttatatgctt tttcaaaagg tttcgttaca aattcatcct cacggttttc caataatttc
    26761 tctgcccagt caggatcgac gatcatttgc tgtccaattg ctgcaagttc tgcatatttc
    26821 aatacctgct ctacatcttc gcgattgcgg atcccaccga caccaactaa agggatcttt
    26881 ccttgaatcg tgttatggac cacttcaaga atcgatttat cgttgtattt atcagaacga
    26941 gctattcgat catacatatt taatgaaaca tgcagataat ctagttttga ctcacgtaat
    27001 cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg gtgtttcata ttcttctggt
    27061 gagaaacgat accctacgat gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc
    27121 gttgttaata attcttcaat aaaacgataa cgattttcca agctaccgcc ccattgatcc
    27181 tctcgtctat tcgaatgcgg agagaaaaat tgttggatca aatacgtatt tgctccatgc
    27241 aactcgattc catcaaatcc tgattcaatc gctctacgtg tggctttgcc aaatgcttgg
    27301 attgtttcaa cgatttcttc ttcactcatt gctcgtggtg tttccgcatc aggacgtttt
    27361 gctgcaactg cacttgcaga tacaggctgt tctccagata aagttgctcg acttgtcatt
    27421 cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt ctccttggat tgctttcgct
    27481 agctcactta atcgaggaat catttcatca tgagcgatgc tgagttcacc tggccagcct
    27541 tttccgatat cttggacatt tgcagcacca gtaataacag caccaagacc tttagaacgt
    27601 tgtttataat aagtgatctc atcttcggtg atcactccat tgaaaaagct gagttgtgtc
    27661 gtcataggcg acagcatcaa tcggttcttc aattgaactc ctgaaggtaa agtaattgat
    27721 tgatcaaatt gtgacattgt cattcctccc ataattcgct ctgattaacc tatcgagaat
    27781 catctatcat taaaccactc gattagttgt acatgcaact aatttgctta aaaaatttta
    27841 ttttttttgt aacttatctg ttgcatcatt cattgtctgg gctaattctt tacttgcttc
    27901 atttcccaaa actgctttgt atgactgatg aagttcttcc cagctaccat cgacttcctt
    27961 agcaaattgg cgtccttgtt ctgtaagata aactaatgac aagcgacctt cgctaactgt
    28021 tttgacgtat cctttatatt ctaatttttc aacaaaacga gcaatcgttg atggggcgga
    28081 atagagcatt tgtgcaatat ctttttgtgg tattccatct ttttctttta atatcaataa
    28141 aataaaacca taagttggtg ccagacctgt ccttctaaac gcttcatcag ctaatttatt
    28201 caacatgcga tctagctttt tcacgctaaa gaaaagacat tcatctaacg ctttttcaat
    28261 tggcttttcc atttcatccc ctccattggc gtacatatca tagtatgatt tttatttttt
    28321 gtcaaaaaac acggaataat gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc
    28381 atcttcaaag aatttcggct tgttttcgac aaaactcctt ctggaacacc gtgaatcaga
    28441 ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc
    28501 acatttttgc ataaatttgg cttaataccg aaaaactggc ttttgaacac cgtttatatc
    28561 agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    28621 ctcatatttt gtcgtttttt gagctaatgc cgatggtcag ctgattgtac accgtttatt
    28681 gagaggtcgt gagcctgccg ttgaactccg agtcacaagg agcaatttca aaaaacagct
    28741 tctcatgttt tatgactatg gtgacttgtg atcgaggagt tggcaggaga atactgttta
    28801 ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc ccttatttcc ttttattata
    28861 ggatttcgcc atttgattgg atgacttgtc tgtaccaatc aaatgatttt ttctttaacc
    28921 gtttctttgt gccattccct tcgtcatctt gatcaacata gatatatcca taacgttttg
    28981 acatttgtga agtagaagcg ctgacgatat caatgcagcc ccacgacgta taaccaaata
    29041 aatctactcc atctttgata gcttccttca tctgctcaat atgctgcttc aaatagtcaa
    29101 tgcggtaatc gtcttgaacg gttccatctt cttctacttt gtctatagct ccgagtccgt
    29161 tttcagctat gaacagtggt ttttgatacc tatcatataa ttgattcaaa gcaattctca
    29221 aacccttagg atcaatctgc cagccccatt cacttgatgg aagatacgga tttttaattc
    29281 ctccagttat taagtttcct tggacccctt cccaattttc tggatgagca ctgactgcac
    29341 tagacatgta gtagctaaaa gaaacaaaat caacagggta ggctgctaag atttcttcat
    29401 cacctacttc tatttttaag ttgacacctt tttcttcaaa cattcgttgc gtatacgctg
    29461 gataatatcc tcttgcttga acatcactaa agaacaaaga agttctttgc tgctcaattg
    29521 ttttttgaat atcatccgga tcacaagaat acggaacaag aagctgatag ctgatcatac
    29581 aaccgacttg tgaacctttg ataatttcat gacaagcttt tgtagctaaa gcacttgcta
    29641 taaactgatg gtgtgcacct tggtatttcg cttgttccaa atggggatgg ttttcttcaa
    29701 ttactcctac gctaacaaaa gggtgatgtt ttacacagtt gatctcattg aaagtcatcc
    29761 aatattttac tttattttta tagcgtttga atactgtttc agcgaaacga acgtaaaact
    29821 gaataagctt tcgattaggc catccaccat attctgtaac caaatataac ggcatttcat
    29881 aatgggaaag agaaacgact ggctcaatcc catattttag acattcgtca aacacttgat
    29941 cataaaaaaa taatcccgct tcattgggtg tttcttcatc accatgagga aaaattcttg
    30001 tccatgcgat agacatccga tagcatttaa aacccatttc agcaaataaa cggatgtctt
    30061 ctttaaagtg atgatagaaa tcatttccgt gtcttttagg ataataatac ttgctgttgg
    30121 gatcctgcgc cgcttttact tgctgatgcg tcatcatatg ccattgatct gtccattttt
    30181 ctttaggtaa atcgctgtcg aatgtataca catcagacac agataatcct ttttcatcta
    30241 catcccatgc accttcacat tgattggccg ctgtcgctcc gccccataaa aatccttctg
    30301 gaaatttttt catatcgatc cccctaaact tgctagtttt cttgtttgat tatttttctt
    30361 atgactagta atgatatttt tgaatctact actatttgta acaataacca ttgtggtcgg
    30421 atcatatccc tctttgatta attcttgcaa atctacttcc atcaataaat cgcctttacg
    30481 tacttttttt ccatcagata ctgcactttg aaagtatttg ccatttaaat tcactgtatc
    30541 aataccgata tggatcagta tctctgctcc ttcttcactg actaccccaa ttgcatgttt
    30601 cgttggaaaa actgttgaaa cttccccgtt tacagaactg tagatttttc cgtccgttgg
    30661 aatgatcgca aacccttctc ctaaagatcc actagcaaat gttggatcgt ttacttcaga
    30721 taaatcaatt atttcacctg ctgcaggagt acttaacact atatcctttg aggattcttc
    30781 ttcgatttca tccttgtagc cgaaaagaaa agttagcata caggaactga tcatcgctaa
    30841 gaaataacta gcaactagcc aaacaaatcc ttcaccaata taaatcggga aagacaaaat
    30901 acttcttgta gcaactgcca aagcaccggc tcctgcggca ccagctactc caccaaatat
    30961 agctccacat atacaagcaa tgtaaaatgg tcgtttatat ttaagagtca caccataaac
    31021 agcaggttca gtgattccaa ataatgctgt gataaatgca gataaagata aactttttac
    31081 tttcttattt ttcgttttca aaaatacccc aagtactgct cctgcttgcg cagctacggc
    31141 agtcatacat gctggaccta gcgtatcacg accgtagaaa gaaagattgt tggtcattac
    31201 tggtacaagt ccccaatgta acccaaaaat cactaacact tgccaaaatc cgccgataac
    31261 agcaccagat aaaacagggc taaagttata aacagctaaa tatccagctg caatcagctg
    31321 acttgcataa tctgcaattg gacctacaac taataaaata attgggacca tcacgatgat
    31381 acaaatgaat ggtgtcaata aatttcgaat actttcgtgc aagcttttgc gaatcttagg
    31441 ctcaatataa gaaagaaccc atacagctaa aattgctgga ataaccgtac ttgtatacct
    31501 tgctagaatc acgggcatcc ctagaaatcg caaggttatg ctatcactat atgcactgac
    31561 aatcgtagga taaacgagag ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa
    31621 cttttttgca gcagtatatg ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc
    31681 tgcattcaaa atgatataag ttcctgattc agtagttaat aaattcaaac tcgtacataa
    31741 ggcgagtact cctttgatca aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac
    31801 agcggcgatc atgtctataa aagagtttaa aatgcctttt ttctcagttc ctctctttcc
    31861 actagatgca tcatcttcta gtttcatttg tttggataat acgtcataca cttcatttac
    31921 tgcatttcct actacgactt gaaacattcc tccagcttcg actacagtga tcacaccttc
    31981 tagttgctct accttctcct tatctgcctt cttattatct tttagggtaa agcgtaacct
    32041 tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc
    32101 tttcgctaat tgtgaatagt ccatttttac ttcccccttg ttaattaatg ccgtagcaat
    32161 ccttcttaca agataagtat agttcttaga aaacggtttc tccattcaat tagtacccct
    32221 tctaatagcg aacgaaactg tacaatagtg tgtatggttt acttagccga gcaaatcact
    32281 cactgcttga aagaaaacaa gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac
    32341 acgcttgatc ttcctttgct gatgtataag tggtatctca agctgatact ctgtgatttt
    32401 tgtttctact tggcttttga taagacagca caacaaagtc tcggcattta acggaagcat
    32461 aactaggtca gaactaggag agattagttg ctgaatggaa tctatctttt tatggatttt
    32521 tatttgattt tcttctttgt agtaatgatc aagcagtttt gttttctttt tttcttcaac
    32581 tatggtcagc ttttcacgca aaatctgata aatctcccaa gattcaaact catctagaac
    32641 actaggtaga taaatcaaat catcccactc aaaaataagc tcttgctcag tagtcaaaat
    32701 tgcatcatat tccgttattt tgtttgattg atttttgaaa agatacactg gttctatccg
    32761 gcattctttg atccacatgt tcatactttg ttgaatctgt ttttttagtg tattcaaaat
    32821 actaattggc tggttactaa caatcagtat ctccccttgt tttcgaaaag gattcaacgc
    32881 ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa ttgggacgta attgaaaatg
    32941 tattgggaag aaacgccgaa ttaaataaat ttctaaagga taacgggaga tgatcttttg
    33001 tacaaaatga ttcatggtat tatgtccggc atccatcctc gttttcattt gtttgatatg
    33061 ggttagcaaa aattctgttt catggaacaa taacgaaaca ggaagatcta aaaaacgaat
    33121 aatcgtttgc tcaaatgctc ttagattctt ctctatttct ttgttttttt catcattaat
    33181 catcaaataa tttaactcta atagtcgatt ttcaattgct tgtttttctt gattggaaaa
    33241 agaatagggt agttctgatg ataacttttt tgtgagggtc tctaccatcg gaataaataa
    33301 tgtatacctg tttgtttttt ctaaaagttc ttctcttcga agaatggtca aaacgagata
    33361 ccgagaaaat cgtatgaagt cctctcctga aacaatataa ttatatgata taagaacctc
    33421 ttttaatgtc ttttggacag tcggcatgat attggaaaaa attgtactga tttgttgagg
    33481 aaaccttgcg tgttggatca ccgtttctgt tccaacaaga gataagaaaa ttctttttga
    33541 attttcttca gcaatgattt ttagtccttt tgtcgacgaa acttccagtt ctaatttagg
    33601 attgcgctcg atccaacgca aaatcgtttt tatttgtaat gatacggctg tttttgaaag
    33661 aaagaaaatg tctgctatct tttgcatact gacataccct ttttgaaaca ataagtacgt
    33721 cacaatagtt gaagctcgga aatccatgct ggaatagatt ttatgtttcg tcacctcttg
    33781 tatgatttgc tgtttagttt cagattcaat tgattctaaa aaataacctg aacgtttatt
    33841 tgactggata atgattttct tctcgtagat agcaagcata tttttgattt cagtttggat
    33901 cgttttcttg ttggtaccta aagcttcagc taattcttcg cttgttatcc aatgattctt
    33961 atttcttaat actacaagaa tttggagttg tcgtttattt aagtccattt gcttacacct
    34021 cctctttgtt ataaacatac aggaaaaacc tctcaaatga caatagtcac ttgcagaggt
    34081 ttaaaaatca tttgaagaaa gatctatatt caaggttttt accattagac tggataattt
    34141 gtttataata ttcaaaacta tcttttttca atctcctaaa gcttccgctc ccatcatttt
    34201 gtctatccac ataaataaag ccatatcgct tcctcatttc ccctgttcct gccgatacga
    34261 tatcgattgg tccccaccat aagtaaccaa tgatttggca accatctttt attgcctcag
    34321 caatttcctg aagatgtatt tttagatact cacaacgttt tggatcgtgg attcgattca
    34381 attcatctgg tgcttcatct agtccaatgc cattttcaac tatgaataat ggtaattcat
    34441 aacggtctgt caattcattc atgacataac gaaaccctag aggatcaatt ggccaactcc
    34501 aaggttcggg agatttttcc ttcaaaaagg gattatcgaa accatttgct ccgccagtat
    34561 ctacgcgcat ttctgcttct tttttataga cagcgctacg ataataacta aatgcaatat
    34621 aatctgccgt gttagcttgc aagcactgtt cgtctccatc taacattact ggtgcacagt
    34681 cttcttcctt ccatgtcctg taaatataag ctggataatg ccctcggcac atgacatcgg
    34741 agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa tacattctct ggatcacagt
    34801 catatgcata agtcgctaat gcagaaaagc tacacataat acccatctta gcatttggat
    34861 cgattttctt taataattga gtagcacggg cgttcgctag aaacatatga tgacaggctt
    34921 gataaatcac tttttttgtt agtgtagcaa tgggttcaag tttatctcta gcagggaatg
    34981 taaccaatcc agcagctgca taaggcatac gaaaagcatt attgatctca ttaaaggtca
    35041 tccaatattt cactttccct tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa
    35101 agtcgattaa ttttcgattt tgccaccccc catactctcc taccaatgct aagggggttt
    35161 cataatgact aagagtaatt actggctcga tatcatatcg atttaatgta ttgattagtc
    35221 tgtcataaaa tacgagacca gcctcatttg gcttctgttc gtctcctctt ggaaatattc
    35281 gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat
    35341 cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga aagataatgg agtgattcgt
    35401 ctaaagcaat ctcccaaatc tttttttctt cattccactg aatactagga tgctggttca
    35461 tgatcccgat aagcgtatct gtcacattag gtaatttgcc gtcttccaac catgcacctt
    35521 cacattgatt agcagctact gctccgcccc ataaaaaatt ttctggaaat atttcctgat
    35581 ccataactat ctctcctcgc tatcaacaat ttcttacttg aagtatagct cgattcatca
    35641 aaacaagata ttctaactat ttcggcagaa gagaagaaaa aaattgaatg ctactcttca
    35701 tttttaatat ttatatataa caaatacata agacgatccc cgaaagaaaa cactcgttaa
    35761 ccatctaagc aaacgattta ttttacttcc aacacaaatg cttcataagg tcgtaaatca
    35821 actttgccgt ctggtaactg tttcagagga taatttgaca caataaccct taaagcttca
    35881 cgtggaatag tgaacgtctg cttttcactg gacatattac aaataacgag ccattgctgg
    35941 tcttcatata tgcgagaata ggcaaagaca tgctcaggcg tcttttccac taattcatag
    36001 tctccccaga cgatgatgtc atgttttcta cgcaaatcga tcaatttctt gtacatataa
    36061 aaaacagaat cttgatctgc taacgcttct tctgcattga tttctttata atttggattg
    36121 actcgaagcc atggaattcc agtagtaaac ccagcatgtt cacgggcgtt ccattgcata
    36181 ggcgtcctag cattgtctct tcctttcgcc ttgattgatt ccaagatttc ctcttttgta
    36241 aatccagaag aaatcctttc gttatacata ttgattgtct caatatcttt tgcttccgcg
    36301 atatcatcga ttgggcaatt cgtcatgcct aactcttctc cttgatagat ataaggggtg
    36361 cctttcatca tatggagcaa gatagcaaac atcttggcac tttcttttcg atacacatcg
    36421 tcatttcccc aacgagaaac aattcgcgga agatcgtgat tattccaaaa taaactattc
    36481 catccttcat cacctaaaga tgtttgccat ttggataata cttttttcag ttcagagata
    36541 ctcaagggtt tcaaatccca tttgtctttg ccttcttgct gatctaatcc tatgtgttcg
    36601 aattggaaga ccatggataa ttcatttctt ttaggattgg aataaagttt agcgatttcg
    36661 gtagttgctc cccatgtttc gccaacagtc atcagatttt tgtcaccaaa agttgcttga
    36721 ttcatttcct gaagatactc atgcagttta ggtccattac ttgtgatttc tttatccggt
    36781 tctttgccaa tcatatcgat cacgtccata cgaaagcccc caataccttt atcgatccag
    36841 aaattcatca tttgataaat ctcttggcga acctgtttat ttttccaatt caaatcaggt
    36901 tgacgcttgc tatacaagtg cagaaaatat tgccccgttt tttcatccaa ctgccaagca
    36961 gaaccactga atattgagcg caatctatta ggctcttttc cattgaccgg atcacgccaa
    37021 atataatagt cccgataagg attttcttta tttttacggg cctcaatgaa ccacggatgt
    37081 tcatcagaag tatgattcac caccagatcc atgatgatac ggatatttct tttctttgct
    37141 tcttcaatca gttcttccat ttcttccatc gtaccgaatt ctgccatgat tgcttgataa
    37201 ttactgatat catacccatt gtcatcattc ggagactcgt atactggaga aagccaaatt
    37261 gcatcaatac ctaaatacgc cagataatcc aacctggaaa taatcccccg caaatctcca
    37321 ataccatctt gatttgaatc ttgaaaactt cttggataga tttgataaac aactgaatgg
    37381 tgccaccatt tttgttccat gattttcctc ctataaatgg atgtttctat tttttgctta
    37441 atcatttatt ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct
    37501 tgttatcggt aacagaataa aacaaaaaaa cagctgagga aattccctca actgtttttt
    37561 gaaaggattg atttatgcag ggaagaatgc tgatttcaca acttctttat aaacggtgtt
    37621 cactcagctg tcccttggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc
    37681 gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc
    37741 aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct
    37801 tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca
    37861 tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc
    37921 tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc
    37981 aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct
    38041 aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata
    38101 ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc
    38161 atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg
    38221 aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc
    38281 aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat
    38341 gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga
    38401 atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct
    38461 ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta
    38521 gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca
    38581 ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc
    38641 aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata
    38701 aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct
    38761 aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga
    38821 taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta
    38881 aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac aaataagata
    38941 taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc
    39001 aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc
    39061 gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc
    39121 atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt
    39181 tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa
    39241 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
    39301 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
    39361 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
    39421 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
    39481 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
    39541 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
    39601 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
    39661 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
    39721 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
    39781 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
    39841 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
    39901 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
    39961 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
    40021 attgctttcc tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct
    40081 tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac
    40141 tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac
    40201 gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg
    40261 tcagcatcgt tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat
    40321 catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga
    40381 atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt
    40441 ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat
    40501 tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac
    40561 gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt
    40621 tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata
    40681 attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata
    40741 agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt
    40801 tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg
    40861 atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa
    40921 agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc
    40981 ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt
    41041 ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact
    41101 cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa
    41161 atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa
    41221 gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat
    41281 actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt
    41341 cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc
    41401 gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga
    41461 aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat
    41521 ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa
    41581 acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc
    41641 tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac
    41701 taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga
    41761 atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct
    41821 ttattttgtt tgtaagacta gttcacctct ttttgttgca aatgcaacta ttttcaataa
    41881 aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa
    41941 gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt
    42001 cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca
    42061 ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa
    42121 tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga
    42181 gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga
    42241 gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg
    42301 attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga
    42361 tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca
    42421 ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct
    42481 tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc
    42541 ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag
    42601 actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa
    42661 aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag
    42721 cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg
    42781 ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct
    42841 cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg
    42901 aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt
    42961 tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt
    43021 tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt
    43081 catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg
    43141 cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat
    43201 aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt
    43261 gccataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat
    43321 ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca
    43381 ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg
    43441 tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt
    43501 ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca
    43561 taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct
    43621 catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg
    43681 acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt
    43741 tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca
    43801 acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct
    43861 gttcctccat ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga
    43921 atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat
    43981 gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg
    44041 tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct
    44101 acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct
    44161 ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct
    44221 ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct
    44281 tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg
    44341 acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga
    44401 agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt
    44461 tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt
    44521 tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta
    44581 atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct
    44641 catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata
    44701 ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag
    44761 tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc
    44821 tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt
    44881 tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta
    44941 aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc
    45001 tacatcggtt gctgtagcat tgttgaatcc catcgaatta aagatcgtta ctagcaaagc
    45061 gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc
    45121 gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc
    45181 aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat
    45241 cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca
    45301 atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac
    45361 gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg
    45421 gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa
    45481 tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga
    45541 caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt
    45601 tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat
    45661 cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg
    45721 cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa
    45781 tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa
    45841 aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt
    45901 gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat
    45961 tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt
    46021 aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac
    46081 ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg
    46141 cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt
    46201 ttcgatgata aaattgataa cgcccatg
//
LOCUS       DACCVU010000009        45021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCVU010000009 DACCVU010000000
VERSION     DACCVU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.009"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            46..2643
                     /gene="adhE"
                     /locus_tag="IVW29_000427"
                     /gene_synonym="adhC"
     CDS             46..2643
                     /gene="adhE"
                     /locus_tag="IVW29_000427"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7837934.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2872..3978
                     /locus_tag="IVW29_000428"
     CDS             2872..3978
                     /locus_tag="IVW29_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7837935.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3995..5023
                     /locus_tag="IVW29_000429"
     CDS             3995..5023
                     /locus_tag="IVW29_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7837936.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5029..5508
                     /locus_tag="IVW29_000430"
     CDS             5029..5508
                     /locus_tag="IVW29_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7837937.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5524..6819
                     /locus_tag="IVW29_000431"
     CDS             5524..6819
                     /locus_tag="IVW29_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7837938.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6830..7654
                     /locus_tag="IVW29_000432"
     CDS             6830..7654
                     /locus_tag="IVW29_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7837939.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7819..8718
                     /locus_tag="IVW29_000433"
     CDS             7819..8718
                     /locus_tag="IVW29_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7837940.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8844..9713)
                     /locus_tag="IVW29_000434"
     CDS             complement(8844..9713)
                     /locus_tag="IVW29_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7837941.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9866..11326
                     /gene="rhaB"
                     /locus_tag="IVW29_000435"
     CDS             9866..11326
                     /gene="rhaB"
                     /locus_tag="IVW29_000435"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7837942.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11323..12579
                     /gene="rhaA"
                     /locus_tag="IVW29_000436"
     CDS             11323..12579
                     /gene="rhaA"
                     /locus_tag="IVW29_000436"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7837943.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12593..13438
                     /gene="rhaD"
                     /locus_tag="IVW29_000437"
     CDS             12593..13438
                     /gene="rhaD"
                     /locus_tag="IVW29_000437"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7837944.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13440..13751
                     /gene="rhaM"
                     /locus_tag="IVW29_000438"
     CDS             13440..13751
                     /gene="rhaM"
                     /locus_tag="IVW29_000438"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7837945.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13833..14984
                     /gene="fucO"
                     /locus_tag="IVW29_000439"
     CDS             13833..14984
                     /gene="fucO"
                     /locus_tag="IVW29_000439"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7837946.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15111..15869
                     /locus_tag="IVW29_000440"
     CDS             15111..15869
                     /locus_tag="IVW29_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7837947.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15969..16613
                     /locus_tag="IVW29_000441"
     CDS             15969..16613
                     /locus_tag="IVW29_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7837948.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16620..17627
                     /locus_tag="IVW29_000442"
     CDS             16620..17627
                     /locus_tag="IVW29_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7837949.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17643..18485
                     /gene="kduI"
                     /locus_tag="IVW29_000443"
     CDS             17643..18485
                     /gene="kduI"
                     /locus_tag="IVW29_000443"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7837950.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18505..19308
                     /locus_tag="IVW29_000444"
     CDS             18505..19308
                     /locus_tag="IVW29_000444"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7837951.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19557..20621)
                     /locus_tag="IVW29_000445"
     CDS             complement(19557..20621)
                     /locus_tag="IVW29_000445"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7837952.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20618..21703)
                     /locus_tag="IVW29_000446"
     CDS             complement(20618..21703)
                     /locus_tag="IVW29_000446"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7837953.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21716..22693)
                     /gene="mvaD"
                     /locus_tag="IVW29_000447"
     CDS             complement(21716..22693)
                     /gene="mvaD"
                     /locus_tag="IVW29_000447"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7837954.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22686..23630)
                     /gene="mvk"
                     /locus_tag="IVW29_000448"
     CDS             complement(22686..23630)
                     /gene="mvk"
                     /locus_tag="IVW29_000448"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7837955.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23952..24605
                     /locus_tag="IVW29_000449"
     CDS             23952..24605
                     /locus_tag="IVW29_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7837956.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24690..25595
                     /locus_tag="IVW29_000450"
     CDS             24690..25595
                     /locus_tag="IVW29_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7837957.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25597..26229)
                     /locus_tag="IVW29_000451"
     CDS             complement(25597..26229)
                     /locus_tag="IVW29_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7837958.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26549..27073
                     /gene="infC"
                     /locus_tag="IVW29_000452"
     CDS             26549..27073
                     /gene="infC"
                     /locus_tag="IVW29_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7837959.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27145..27345
                     /gene="rpmI"
                     /locus_tag="IVW29_000453"
     CDS             27145..27345
                     /gene="rpmI"
                     /locus_tag="IVW29_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7837960.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27398..27757
                     /gene="rplT"
                     /locus_tag="IVW29_000454"
     CDS             27398..27757
                     /gene="rplT"
                     /locus_tag="IVW29_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7837961.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28009..29346
                     /locus_tag="IVW29_000455"
     CDS             28009..29346
                     /locus_tag="IVW29_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7837962.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29394..31523
                     /locus_tag="IVW29_000456"
     CDS             29394..31523
                     /locus_tag="IVW29_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7837963.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31498..32187
                     /locus_tag="IVW29_000457"
     CDS             31498..32187
                     /locus_tag="IVW29_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837964.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32595..33281)
                     /locus_tag="IVW29_000458"
     CDS             complement(32595..33281)
                     /locus_tag="IVW29_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7837965.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33417..33611)
                     /locus_tag="IVW29_000459"
     CDS             complement(33417..33611)
                     /locus_tag="IVW29_000459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837966.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33661..34101)
                     /locus_tag="IVW29_000460"
     CDS             complement(33661..34101)
                     /locus_tag="IVW29_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7837967.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34105..34950)
                     /locus_tag="IVW29_000461"
     CDS             complement(34105..34950)
                     /locus_tag="IVW29_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7837968.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35099..36517
                     /gene="pepV"
                     /locus_tag="IVW29_000462"
     CDS             35099..36517
                     /gene="pepV"
                     /locus_tag="IVW29_000462"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7837969.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36574..37521)
                     /locus_tag="IVW29_000463"
     CDS             complement(36574..37521)
                     /locus_tag="IVW29_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7837970.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37744..38094)
                     /locus_tag="IVW29_000464"
     CDS             complement(37744..38094)
                     /locus_tag="IVW29_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7837971.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38294..39373
                     /gene="pepA"
                     /locus_tag="IVW29_000465"
     CDS             38294..39373
                     /gene="pepA"
                     /locus_tag="IVW29_000465"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7837972.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39517..39837
                     /locus_tag="IVW29_000466"
     CDS             39517..39837
                     /locus_tag="IVW29_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7837973.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39859..40323
                     /locus_tag="IVW29_000467"
     CDS             39859..40323
                     /locus_tag="IVW29_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7837974.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40528..41133
                     /locus_tag="IVW29_000468"
     CDS             40528..41133
                     /locus_tag="IVW29_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7837975.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41172..42461)
                     /locus_tag="IVW29_000469"
     CDS             complement(41172..42461)
                     /locus_tag="IVW29_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7837976.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42889..43368
                     /gene="rlmH"
                     /locus_tag="IVW29_000470"
     CDS             42889..43368
                     /gene="rlmH"
                     /locus_tag="IVW29_000470"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7837977.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43571..44121
                     /locus_tag="IVW29_000471"
                     /pseudo
     CDS             43571..44121
                     /locus_tag="IVW29_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            44192..>45021
                     /locus_tag="IVW29_000472"
     CDS             44192..>45021
                     /locus_tag="IVW29_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP7837978.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT        14315 a         8140 c         9590 g        12976 t
ORIGIN      
        1 tgtgaattat ttaacaaata accatgaccc attaggagga taatgatggc taaagtaata
       61 gaaaaagaaa aaacgaagac aattgacgta caagcaatga ttgatgattt ggcagaaaaa
      121 gcaaatgcag cattgaaaga aatggaaagc tttgatcaag aaaaagtaga ccatatcgtg
      181 catgaaatgg caatggcagc tttggatcaa catatgccat tagcgaagat ggctgtagag
      241 gaaacaggcc gcggtgttta cgaagataaa gcaatcaaaa atatgtatgc gtccgaatat
      301 atttggcata atatcaaaca tgataaaaca gttggtgtga tcaatgaaga tgtccaaaaa
      361 gggttgattg aaattgctga accagtcggt gtggtttgcg gagtaactcc aacaacgaat
      421 ccgacttcca caacgatttt caaatcaatg atcgcgttga aaacaagaaa tccaatcgtt
      481 tttgctttcc atccaagtgc ccaaaaatct tctgcagaag cagcacgagt ggtacgtgat
      541 gctgctatcg ctgccggagc tcctgaaaat tgtatccaat ggatcgaaca tccttcgatc
      601 gaagcgactt ctatgctaat gaaccatcca ggtatcgcga tcgtcttagc gacaggtgga
      661 gctggaatgg tcaagtctgc ttactctaca ggaaaaccag ctttaggtgt aggtccaggt
      721 aacgtaccag cttatattga aaaaacagca aaaattaaac gtgcagtgaa cgatttgatc
      781 gtttcgaaaa catttgataa tggaatgatt tgtgcttctg aacaagctgt gatcgtggat
      841 aaagaaatct atgcagcagt aaaagcagaa ttccaagcac accaagttta tatcgtaaaa
      901 ccagatgaat tgcaaaaatt agaagatgca gtgatgaacg aaggaaaata tgcggtcaat
      961 ccatcaatcg ttggacattc tgcgatggaa atcgcaaaat tggcaggaat cagtgtacca
     1021 aaaggaacga aaatgctgat tgctgaattg gaaggcgtag gcccagatta tccgctatca
     1081 agagaaaaac tttctccagt tcttgccatg atcaaagcta acaacacgga ccatgcgttt
     1141 gatttgtgtg aaggtatgtt gaatcttgga ggattaggac atacagcagt tatccattca
     1201 gaaaacgaag agttgcatgt caaattcggt cttcgaatga aagcttgtcg tattttagtg
     1261 aatacaccat ctgcagaagg cggcatcggt gatatctata acgaaatgat tccgtcattg
     1321 actttgggat gcggttctta tggtaagaac tctgtttctc gtaacgtctc tgctgtaaac
     1381 ttaatcaatg tcaaaactgt agcgaaacgg agaaataaca tgcaatggtt caaattaccg
     1441 ccaaaaatct tctttgaaaa gaactctcta ttgtacttag agaaaatgga aaacgttgaa
     1501 cgtgtcatga tcgtttgtga ccctggtatg gtccaattcg gttactgcga tacagttcgt
     1561 gaagtattgg cacgacgtaa aaatgatgtg aagatcgaag tcttctcaga tgttgaaccg
     1621 aacccatcga caaataccgt atacgcagga acgaaactga tggcagactt caagccagat
     1681 acagtaatcg ctcttggagg cggttctgca atggacgcag ctaagggaat gtggatgttc
     1741 tatgaacacc cagacacatc tttctttggt gcaaaacaaa aattcttgga tatccgtaaa
     1801 cggacatata agatcgacaa accagaaaaa acacaattcg tatgtatccc gacaacatca
     1861 gggactggtt ctgaagtaac gccatttgcg gtcatcactg acagtgaaac acatgtgaaa
     1921 tatccgttag cagattatgc attgacgcca gatgtcgcaa tcgtcgatcc tcaattcgtc
     1981 atgtcagttc cagcatctgt cacagcagat accgggatgg atgtgttaac acatgcaatc
     2041 gaatcctatg tttcagtcat ggcttcagat tatacacgtg gattgagcct acaagcaatc
     2101 aagttagtct ttgactattt agaaaaatca gtaaaaacac ctgatatgga atcacgtgaa
     2161 aaaatgcaca acgcttctac aatggctggt atggcgtttg ctaacgcatt cctaggtatt
     2221 tgtcactcag tcgcacataa aatcggcggg gaatacggga tccctcatgg acggacaaat
     2281 gcgattcttt taccgcatat tatccgctat aatgcaaaag atccacaaaa acatgcgatg
     2341 ttccctaaat atgactattt ccgtgcagat acagattacg cagatatcgc aaaattctta
     2401 ggtctgaaag gcgaaacaac agaggaatta gtcgaagctt tagcaacggc tgtttatgaa
     2461 ttaggaaaat ctgtcggcat caacatgagt ctaaaagctc aaggagtcac acaagaaaca
     2521 ctagatacaa ccgtagaccg tatggctgaa ttagcttacg aagaccaatg tacgacagct
     2581 aatccgaaag aaccattgat ctctgaattg aaacagatca tcattgatgc ttacaacgga
     2641 taagcaaaag aaagcgtaga aaaaagataa gaaaatagat gaaaaagaac ttggaaaacg
     2701 ttgtttctcc aagttctttt tttaatttcc aagtaactga aagttacttt tgttatttaa
     2761 catttaggtc aattttagac agtttttgtt caaaaagcga tatttatagt ggacggtatt
     2821 actgctatat taaggatata aaatgaaagc gtttaaaaag gtggcgtaaa gatgattatt
     2881 aaagaagaga tttttgtacc taaagaatta gtgaaagaaa aaattgacct gctcgttgaa
     2941 aacctgacaa cgatcaaaga cgataatggt gaatttttgt tggactttga tggactgaaa
     3001 gtagatgaca aaagctggac agtgtggaat tggccacaag gtgtcggtct atatggtatt
     3061 tataaaaatt accagatgac aaaaaatcct cgcgcttatc aagtggtcaa tgaatggttt
     3121 gaggatcgta tggaagaagg cgctccgccc aaaaacgtca atacaatggc accgctattg
     3181 actatggctt acttgtatga agacacgaaa gattcacgct atttgcctta tttagaacaa
     3241 tgggcagaat gggtcatgaa tgacatgcct cggacaaatg aagacgggtt acagcatgcc
     3301 acatatggac cggaaaataa gaatcagttg tgggacgata cattgatgat gacagtgctt
     3361 cctttggcaa aaatcgggaa actgttgaat cgcccagaat atttagaaga agctcgttac
     3421 cagtttatga tccatatcaa atacttgatg gataaacgaa caggattgtg gtatcacggc
     3481 tggacgtttg aaggaaatca taactatgcc gaagcatttt gggctcgtgg aaactgctgg
     3541 ttgacgattg ctattccaga gatcattgaa atcttagaat tatcgaaaga agatgcattg
     3601 cgcaaattat tgatcgaaac acttgaagca caagtgcgtg cattaaaaac ttatcagtct
     3661 gaaagcggtt tatggcatac gttgttggat gatccttctt cttacgtaga atcttctgca
     3721 actgcaggat ttgcttacgg tattttgaaa gcggtccata aacgttattt accacaagaa
     3781 tataaagaag tcgcttatcg tgctatccaa ggattgcttg aacaaatcga tgaaaaagga
     3841 gaagttcaaa acgtttcaat cgggacagga atgggggaca gtttagattt ttatcggaat
     3901 atcggaatca cagcaatgcc ttatggacag tcgttaacgg tgctttgttt gacagaattg
     3961 ctcgtctctt attgttaaaa gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc
     4021 agcaagttcg cttagcctag gtctgctagg tctaagcggc tgcggaaata attctagtgt
     4081 ggcaactgtc gcagatgcag atgtaaatga gatagttacc ttgcgctttg cttatgcaag
     4141 caacagccag ccagtaattg atgcaatgaa tgaatttggg cgtttagtaa aagaaaaaac
     4201 agatggttcc gtacagatcc agtatttccc agatggacaa ctgggtggag aacgtgaact
     4261 gatcgaattg acacagtcag gagcagtaga tttcacaaaa gtaagtgcct cagcgttgga
     4321 aagtttctct aaagattatt cgattttttc agttccttat ctattcaatg atgaagaaca
     4381 tttctttaag gtgatggata atgaagaaat aatggagcca atctaccaat cgaccaaaaa
     4441 tttaggattt accggattga cctattatga ttctggtcag cgcagctttt atatggtaga
     4501 cggtccgatc catacgccgg aagatttgaa gggaaagaag atccgtgtga tgcaaagcga
     4561 aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg gaagtgatga
     4621 agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg aatttgtctt
     4681 gtatacagct ggacatggtg gtgtggctaa atactattcc tatgatgagc atacacgtgt
     4741 gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg agcaggaaaa
     4801 agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt ttgcaaaagc
     4861 aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca atgatgtaga
     4921 taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa acgatcctac
     4981 ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtgat gaaaatgatt
     5041 ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt cgctgttgtt
     5101 ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac ggaagagctt
     5161 gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt gggacaaaaa
     5221 gcacatttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag taaaatttta
     5281 gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt gtatggcgga
     5341 tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat tcctatggga
     5401 tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag cctgatcaat
     5461 ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa atggaggaat
     5521 aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt gcttgtatta
     5581 gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt gttagttata
     5641 ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat caatagtttt
     5701 tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa tggagggata
     5761 gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg ggctttggct
     5821 catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc cattgccgct
     5881 tctaccgcga ttggcggagt gttgatccct cagcaagtgg aagaaggata tgaccggaag
     5941 tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc tccaagtact
     6001 gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt gtttatggga
     6061 ggttatctcg taggcgcttt gtgggcgcta ggaatcatgc tagtagcatt catcgttgct
     6121 aaaaagaata aataccctgt tgtcgaaaaa ggacagatgg ctcatgctgg aaaaataata
     6181 aaagaggcga ttcctagcct tctattgatt gtcatcatta tcggtggtat cttgacgggg
     6241 atatttacag cagtagaagc ttcagcgata gctgtcattt actccctgtt gattgctatg
     6301 ttttactata aaacggtaaa actaaaagat atacctaata tgttgtttca atcagtagca
     6361 atgtcaggga cgatcatgtt cttattggct acttcttcca tgatgtcttt cgccatggcg
     6421 tttaccggta ttcctcaggc aattagttcg ttgattatgg gcgtaacgga taataaattt
     6481 attattctgt tattagtcaa tcttgtgtta ttactggtgg gtatgtttat ggatgtggcg
     6541 cctgctatct tgatttttac accaatattt ttaccaatcg tcacaagtgt aggggttgat
     6601 cctgtacatt acgggttgtt ttcaatcatg aatctgtgtg taggctccat cacgccgcca
     6661 gtaggtacag gactatatgt cggtgcaagt gtaggaggcg taaaagcaga ggcaatgctg
     6721 aaaccgcttc ttccttttta tggggttatc ttagtcgtct tgtttttgat tacttacttc
     6781 ccgcaaatcg tcatgtggct acccaatatg ctgagttagg agaatagtta tgttgaacat
     6841 tacaattaga ggacatgatc tttccgaagt ccaaacgata gaagatcttg cagaaaaaac
     6901 aaaagaacaa gggattcata cactgcagct cgccttaggt ctttcgtttc ctgaaatacc
     6961 ttctggcgca aatgagatca attctgggat gggaaattat gtcaaacgag ttttagaaaa
     7021 gcaagaagtg accgtaggaa ttttaagttg ctatatcaat atgattcatc cagatttaac
     7081 gatacgacaa gagttgctga caaagttcga gcagtatctt cgctatgctt cttcctttgg
     7141 tgcgtcgatg gttgcttcgg aaactggatg tgttttgccg gaaatccaat atacggaaga
     7201 aaatttcaca gatgaagcat ttgctgaagc tgtgtccgtt atccgtcgct tggtaaaagc
     7261 tggagaaaaa taccaaatga tggtggggat agaaccaggt ttgaatcatc cggtgtattc
     7321 tttagcaagg gtagagcagc tgatccaagc agtagattcg gactatctag gaatcatttt
     7381 agatccgacc aatttgatca caagtagtga ttatcaagaa caagtccaat tggtagaaga
     7441 agcttttgaa cgttttggtg aaaagatatg tgcggttcat ttgaaagatt ttcgtgtcga
     7501 acaggaaaaa atcgtgccgg taaatttggg agacggtgtg atcgagtaca caaaaatcaa
     7561 agaaatcatc atgaagaatc gtccgtacct gtatgtggtt cttgaagaaa caaaagatga
     7621 tgggattcgt tatggaagaa gtcttcttga ataatgtaaa gaagacgttg gttaactaat
     7681 atttagaatg gagggggcgg ggctagaaga tgccacgtct cttctttttt ataagagtat
     7741 ataaaagcag ctctttttga ttacaaaaaa cgtggtataa taaaattgtg cataagtgaa
     7801 caaaaggagg tgcggcacat gctccaaaag aaaatcagcg gtgaacaagt caatacgatg
     7861 gtttctgatc agtatgaaat ctatcatgtc aaagatgtcg tcagccaaaa taaaacgatc
     7921 tatcatcatc atgatttcta tgagatccat gctacattaa agggagaagc gactttttat
     7981 ttgaacggac gacaatttac gatcaaagca ggaagtgtgc tgctcatcca ttccaatgat
     8041 ttgcatcgta tcgtccgcca atcttcagat gtattcgagc gtgtctatat ctttgtcacc
     8101 ccttcttttc tagaaagtcg ttccacgcca tggtctaatc tatcggcttg ttttcaacca
     8161 atgggagaga agaggagtcg tgtattgcaa atagaacagg aagtattgaa gcaaaaatta
     8221 tcatttatcg accatcctcc tgataaaaaa atttacggtg aagacataaa atatgaacaa
     8281 gctctggtag aatacttgat ttttctcaat caagttgtac aaaaagaaga gagtgcgcaa
     8341 gaacagaatg ctgtagttcc taatgagcgt gtggaacaaa tgttacaata tatctcaaaa
     8401 aatttatctc atccgctttc tttagaggaa atggaaaagc agttttttgt cagcaaatac
     8461 catatcacac gagaatttaa aaagtacact ggatttacgt ttcatcagta tgtaatgaag
     8521 aaaaagctga tctatgccaa gcacttgttg cgagaatacc gcagctctag tgctgtatat
     8581 agtaaaagtg gtttttcttc ttatccgcat tttctgaaag cattcaagag agagttcggc
     8641 ataacgccta aagaattttt gaaacgtgat acacagaaac agttggttca ttatgaacat
     8701 tttgaagatg aacaatgaga atgatcacta aaaaataacc gcagtagctg ctgttgttta
     8761 catagaagca gaaactgcgg ttattttatt tttatctata tatttcaaaa tattttgaaa
     8821 aaatgacgta catattatat gaatcagctg aatttacgaa agtctctcgg cgatagtcct
     8881 gtttttgctt tgaacaggcg gctaaagtaa gcaaaattct caatccctat ttcttcggaa
     8941 atcattcgaa ccgtaaattc tgtataccga agaaggtctt tagcgtaaga aagttttttg
     9001 ctgatcaaat aatcgattgg tggagaaccg ataaatttgg aaaaatcttt gctcagctga
     9061 tatttattca ataagaattg ttgttctaat tgatcaagtg taatcgtttc tttgaaatgt
     9121 tcgtctaagt attgcttcag tttgtaaacg taggcgggaa tatcctcttc ttcgaaatct
     9181 agctgatact tttgcaaaat cagttcattc agcaactcgt ggatagctac agaggactgg
     9241 aaatcggtac gggcattcgt gccttgttgc atgtggatca attctgtgat caaatggtgg
     9301 atcttgtttt ctgatggatc tcctgttgaa tggaaggtgt tatggccgtc ttttgtgaac
     9361 tcctgataaa atgattggac gttctgacca ttcaaatgga tccaatccat ttcccatggt
     9421 tcttcgctga tcgtttgata acgctgatat tcttggcagt caataaaaaa taagtctcca
     9481 gcttttagct gttgctgatt atcattatat gtaagcgaac ctttgccaga caaagtaaac
     9541 tttatcaagt aagaaggtaa atgtgctcgt tcagtatagt aagggattgg tgctttgaag
     9601 taaccgattt cttggatata aaaaaataaa ttcctgcttc tttgtgtagg cgtcaagatc
     9661 actctttttg agtcttccga ccatgagtgg ttggcttttt cccagtaatc catgtaaaca
     9721 ctcccaactt gtcaagataa tgcaattata taccaaaata gtgtgaagca aaatataaga
     9781 ttctgtatta tgatagcatt gtcaataaat gataacgctt tattaataaa gtgctaatca
     9841 acaataaagt aaaagaggtg gagaaatgaa tccaacattt gttgccatcg atatcggtgc
     9901 atcaagcgga cgactaatga aaagtacgag ggatgcaaaa ggttctttga tcttggaaga
     9961 agtacaccgg ttcaaaaacg gctttcggaa caaagacggt cacgatcgct ggaagatcga
    10021 ttatcttatc aaagagattt tgattggatt tgaaaaatta aagcagacag ggattgatcg
    10081 cctttttgcg gggatcgata catgggcagt cgattactgt ctgattgatg aacaagggga
    10141 tcggataaat gatccgattg cttatcggga caagcgtacc aatggagcag tagaacagtt
    10201 ttctgaaatc tattctctag ataagttgta tgagcagaca gggattcaga tccagccttt
    10261 caataccttg ttccaattgt ttgctgaaga aaaagaaaat ttggcaaaag cacataaatt
    10321 gttactgata cctgattatt tagggtatgt ctttactggt aacatggtga cggagaaaac
    10381 taatgcatct actatgcagc tgctgaatgc agggacaaaa gaatgggaaa tagaacttct
    10441 tgaaaaaatc ggtgtctcga aggaattatt tccaccattg acagagccgg gaacgatact
    10501 aggagatctg caaaaagaac aattttcaaa atatgatttg ccagatgtga cctttatcac
    10561 agtagcaagt catgatacgg cgtcagctgt attaggaacg ccaggtcaag gagaacgttg
    10621 gggttatatc agcagtggga catggtcgct tttagggatt gaaacaaaga tcgccaatgt
    10681 ttctcgcaat gctttttctg aaaattatac gaatgaatgg ggtgcccata acacggtgcg
    10741 ttttttgaaa aacatcatgg ggatgtggtt gatccaggaa gttgcccgaa tgcaagagta
    10801 cgagtacagt tatgcggaat tggcagagct tgctgcaaaa gaaaaaccgc tgcagcaagt
    10861 gatggaaatc aatgaccctc gatttttgaa tcccccaaat atgatagaag agattcagca
    10921 atactgtttt gaaacagagc agccagtccc tgtctcatcg ggagctatcg ctcgttgtgt
    10981 gtatgataat cttgctcttt gttacgcaca agagctgatc aaagtagaac acttaactgg
    11041 aacgaaattg gaaaagctgc acatcgtagg gggcggatcg aataatcgtc tactgaatca
    11101 attgacagca gattatgccg gaatcattgt agaagcagga cctagtgaag ccacagcaat
    11161 cgggaatgtt gccatgcaaa tgctcagtac aggtcagttt tcgaatatag aacaagcaag
    11221 aaatgtaatc aaacagtcgt ttccatgtga aactttttac ccagatgaaa ataagcaaaa
    11281 cacgatggaa tggtacgtga ataaaatcaa aggagtaatc gtatgaacac gattgaaaga
    11341 aattacgaac aagcaaaaga aaaatatgca actatcggtg tagatacaga tgctgtacta
    11401 gaaaagatac aggacatcaa aatttctatg cattgctggc aaggagatga tgtcaaaggt
    11461 ttcttgacac cggatggcga attgaccggt ggaatcatgg cgacagggaa ttttccagga
    11521 gccgcacgaa cacctgaaga gcttagacaa gatctggaga aggcttattc gttgataccg
    11581 ggcaaacaca aattgaacct tcatgctatc tatttggata cagaagaatc tgtcgatcta
    11641 aatgaaattg agccgaaaca ttttgaaaaa tgggtagagt gggcaaaaaa agaagaaatc
    11701 ggtcttgact tcaatccgac tttcttttcg catccaatga tgaaagacgg ctttacattg
    11761 gctcatccag ataaagaagt tcgtgaatac tggatcgaac acgggaaaag aagccgcaaa
    11821 atcgcggagt actttgggaa agaaactggg caaacaagta tcaataactt ttgggtacct
    11881 gatgggatga aagataatcc gattgatcgt tttaccccac gggaacgttt aatggaatca
    11941 ctcgatgaaa tttttacaga agagttagat gaacggtatg tacaagatgc agtagaaagc
    12001 aaactatttg gtttaggcgc tgaagcttat acagtcggtt cccatgaatt ttatatgggt
    12061 tacgggttga ctcgtgacaa acttgtttgt ctagatgctg gacattttca tccaacagaa
    12121 gtgatttcca ataaattatc ttcattagct ttattcagca aaggaatcat gttgcatgtt
    12181 tcccgtccag tgcgttggga ttcagaccat gtggttttga tggatgatga attgcaggaa
    12241 atcgctaaag aattagtccg taacgaattg ttggaaaaaa caaatatcgg tctggatttc
    12301 tttgatgcaa cgatcaaccg tatcgctgct tgggtcatcg gtacgagaaa tacacaaaaa
    12361 gcactgctga aagctatgct ggaaccagtt gagcgattga aagaaatgga gctggctttt
    12421 gactttactt caagaatggc ttatacggaa gaattgaaag atttcccgta tgccgatgta
    12481 tggaattatt tctgttacaa aaatcaagtt ccagttggtt tggattggct ggaagaagtc
    12541 caggaatatc aaaaagacgt gttggaatta agaaaatagg agtgtggcta tattgaaaaa
    12601 aatagatatt ttacaagcgc cttatgtgaa agaaatgatc gaaacgacgt cgaatcttta
    12661 tcgcttggga tgggatgaaa gaaatggcgg gaatatttct tatttactaa aagaagaaga
    12721 gatcacaccg ttcttggatc ctgaaaaagt gatccgccgc atctcgatga tttttgatgc
    12781 cagccgttta gcaggatgct attttattgt aactggttca ggaagatatt ttaaaaatgt
    12841 agcgagcgaa cctgccgaga atcttggaat cgttcgagtt gctgaagatg gcaaaacact
    12901 ggaacttttg tgggggctgg aaaacgaatg ccttcctaca agcgagttgc caagtcattt
    12961 catgagtcat atcgcccgtt tagaagtaga cccagataat cggatcgtta tgcattgcca
    13021 tgctagtcat ctattagcga tgagttttac gcatgaatta gacgaacgtt cctttagtcg
    13081 tacgctatgg caaatgtgta cggaatgctt agttgttttt ccagaaggaa tttccatcat
    13141 tccgtggatg gttccgggaa caaatgagat tggggaagca acagctgaaa aaatgaaaga
    13201 aactcgattg gttttatggc cacaacatgg aatctacggg tcaggaaaag acatggatga
    13261 agtctttggt ttgatcgaga cagcagaaaa ggcagcagaa gtctatacgt acgtcaaagc
    13321 gcaaggacca atcttgcaaa caatcacgga cgaaaacctg tggcatttag cagatgcgtt
    13381 tggtgtcact ccaaaagcag gttatttgga ggaagttcat acgaaagcag gtgtctgaca
    13441 tggtgaaaaa agctttttgc atgaaagttt acccagaaaa acaagaagaa tacaaaaaaa
    13501 gacatgatga actttggcca gaaatgcgta cagcacttcg tgaacacggg gtcatcgact
    13561 attcgatcta tcttgatccg agtacaagtt tgttatttgg ttacttagtg atcgaagatg
    13621 aaaagttatg ggaaaaaatg gcaaatacat ctatcaatca aaaatggtgg aagtacatgg
    13681 aagatatcat ggagacaaat ccagatgcct ctccagtcag ttgggaattg atgccagtct
    13741 ttgaactgta gcaacaagca actgggcaac cggttgcttg gggttctttc gtttttattg
    13801 tgaaaaataa tacaaattag gaggaataga aaatgagtaa tcgaatgatt ttaaatgaaa
    13861 catcgtattt tggaaaagga gcgattgagt cgatcgtccc tgaatttcag cagcgaggat
    13921 tcggcaaagc agctgtcatt actgacaaag gattgatcga gcacggtata gcaacaaaag
    13981 taacagaact tctagataaa gcagcaatcg cttatgcttt atacgacggg atcgtaccta
    14041 atccaacgat ccaaaatgta aaagatggag tggcttttgt taaagaggca gaggcagact
    14101 gtttgatcgc aatcggtgga ggttcgccaa tcgatacggc aaaagccatt gggattattt
    14161 tgacgaatcc agagttttca gacgtcatta gtttagaagg tgttgctgat acgaaaaatc
    14221 catgtttacc tatattagct ataccaacga cttctggaac agcagcagaa gtaacgatca
    14281 actacgtcat cacagatgaa gtaaatcacc gaaaatttgt ttgtgttgat cctcatgata
    14341 tcccaatcgt cgcgttcatc gacagcgata tgatgatggg aatgccgaaa aaattggctg
    14401 catcaacagg tatggatgct atgacgcatg ctattgaagg gtttatcaca aaaggcgctt
    14461 gggaaatgac tgatatgctg catttaaaag caatcgaaat catcggccat tctttggaag
    14521 cttctgtaga tggcgatcaa aatgggcgag aaaaaatggc attaggacag tatattgcgg
    14581 gaatgggctt ctcaaatgtc ggtttaggac tggtacacgg gatggctcac ccgttaagtg
    14641 catggtacaa cattcctcat ggcgtagcgt gtgcagcact tttgccaact gtcatgaaat
    14701 ataacaaaga atatactgga gaaaagtacc gtgagatcgc tcttgttcta gggattaaag
    14761 gagcagctga aatgtcttta gaagatgtaa gagaagcggc ttgtggtgag atcgatcggt
    14821 tgagcaaagc ggttggaatc ccagaaacca tttctgaatt aggtgtaaaa gaagcagata
    14881 ttccagcaat tgcagaagat gctttgcgag atgtatgcac accaggaaat ccaagagaaa
    14941 cgacagtaga agaaatcatt gctttatatc aaagtttgat gtaaaaacga agcggatctt
    15001 caacattaaa ttgcctgtaa agagaaatgg tagtttatat ctggatatct cttttaaatt
    15061 gatggtattt ccgctataat ttttcttata aagaaatagg agaatattat atggaaaata
    15121 aaaagcctta tggaactgta ttgatcaaag cctctaaaat actagactat ttagcggaaa
    15181 accaagatgt ctctttgcag acgatcgcaa agggtgtggg aatgacctct tctacagtct
    15241 tgaaaatact agatactttg ttgatgatcg gctacgtaaa taaaaactca gagaaaaact
    15301 atcgtttagg tgcaaagctt attcgctacg cgaataaaaa cattgaacaa atcgatttgg
    15361 tagagttgac tttaccgttt ttagaacagc tgcagcaaaa gatcgatgag actatccact
    15421 taggggtatt ggacaacaat gagatcttgt acgtgaacaa actagatcca aaacatcaaa
    15481 cgatccgcat gtcttcaaaa atcggaatca cccgaccatt atacaattct gcgatgggca
    15541 aagccgtatt agcagaattc agtgaagagc agtacactga ttatcttgaa aagcatccgt
    15601 tgatccctta cacagaatat acgatcacca atgcattacg gttgaaaaag gaaatcaaaa
    15661 cggtaaaaga aacccacatc gcatttgacg atgaagaaat cgaacgtgat attttttgta
    15721 taggagcctc aatcgtgaaa aacgaacaaa tcattggtgc atttagtgta agtatgccga
    15781 aatatcgttt aacagatgag acaaaagaac aaataatcag tgccctcaag caaacaaaaa
    15841 gcgaaatcga gcaaagaatt caaaaataat gaaaaaagcg tttgcaattt ttcttttctg
    15901 ccgtataata tgggtataga tttccaagtt ataaaataaa atttctaaat aggaaatagg
    15961 tggagaagat gaaaaaaatt gatattttat ctcgattgaa aaacgccgga gtgattgcgg
    16021 tggtacgcgg aaaaagcaaa gaagaagctt tgaatgcttg ccacgcaatc atcaaaggtg
    16081 gtctaactgg tattgagttg acttttacag tccctcaagc agatcaagta atcaaagaac
    16141 ttttgtcatt ctacaaagac caaccagaaa tagtgattgg cgcaggcaca gtattagacg
    16201 ctgtcacagc tcgcttggct atcttagctg gcgcagagta tattgttagc ccttcctttg
    16261 atcaagaaac ggcagagatg tgcaatctgt atcaaattcc ttacttacca ggatgtatga
    16321 ctattaccga gatcaaaaca gcgctgaaaa gcggggtaga tatcgtcaaa ctatttcctg
    16381 gaagcgctta tggaccaagt atcatttctg ccttcaaggc gccgatgcct caagtcaata
    16441 tcatgccaac tggcggtgtg agtctggaca atatgaaaga gtggttcgat gctggtgtcg
    16501 taacagtcgg agtaggtggg aaccttttag cacccgcagc tacaggagat tttgacaaag
    16561 taacggaagt cgcgcaacag tatgcagcaa aaatgaaaga aatcaagagg tagctgctta
    16621 tgggaaaagt agtgacactc ggagaaatca tgttgcgatt gtccacagag cagggaaccc
    16681 gtatcgattc aagtgaacga ttcgctgccc attatggcgg aggggaagca aacgtagcaa
    16741 tttctttagc taattacgga catgaagcga tttttgctac aaaagtacct gataacgcat
    16801 tgggaaaagc agtcaaaaag cacttgcaat gttatggagt agatacgaaa caggtattat
    16861 ctggtggatc aagactaggt acttattata tggaaactgg tagtggcgaa cgcgccgcct
    16921 cagtgatcta tgatcgtgcc ggatcaagtt ttgctgaaat ggatcatttg gaatggtcgc
    16981 tagaggagtt attccaagga atagatatat ttcatgtttc aggaatcacc ccagctttgt
    17041 ctaaaaaatg gcaaaaaatg acgaaacagg tgatagaagc tgcaaaaaaa gctggctgcc
    17101 tgatcagctt cgatatcaac tatcgtggaa aattatggac acagcaagaa gccagcgaag
    17161 taatccatca gcttttacct ttagtagata tctgctcagc tggaaaaatg gatgctcttt
    17221 atttgttagg tatcgagaaa gcgccagaag cagaagaaca acctctaatt tattattatc
    17281 aaaaaatgca ggaaaagttc ccgaatattc aggtgtttta ttcgaccaag cgtaccgttc
    17341 attcagcaag tgaaaatgaa ttgaagggga cattgtggat gaaccatgaa tatgtggaat
    17401 cccaagtcca tagaatcacc ccaatcgtgg atcgagtagg tggcggagat gcttttgcag
    17461 gtggcgtgct ccatgggatt ttatccaata tgtctccgca agaaatcatt gattttgcta
    17521 cggcagcttc tgccttgaag catacgatcc atggggactg caaccaattt agtcaagaag
    17581 aagtcgcaca atttttagca tgcggctctg gaaaaatcat tcgatagaag gagtagaaga
    17641 aaatgcaaga aatggaaaca cgttatacac acagtccaga agatatccgt cattattcaa
    17701 cggaacaatt gagaaaagaa tttttagtag aaaaggtatt tgaaccaggg aaaatcagtt
    17761 tgacttatac gcacaacgat cgaatgatct ttggtggagt gacacctact gaaaaagaat
    17821 tagaaatcac attagacaaa gaactaggtg ttgattactt cttagagcgt cgtgaactag
    17881 gtgtgatcaa tatcggtgga ccaggattca tcgagatcga tgggcaaaaa gaagaaatga
    17941 aaaaacaaga cggatactat atcggaaaag aaacacgaca tgttgtattt tcttctcaag
    18001 atgctgaaaa cccagctaaa ttctatatta gttctgttcc agctcatcat aaatatccaa
    18061 atgtaaaaat cagtatcgac caagtaaaac caatggagac tggtgaaggc ctgacactga
    18121 atgaacgtaa aatctaccaa tacatccatc caaatgtatg cgaaagctgt caattacaaa
    18181 tgggttatac gattttagaa ccaggaagcg cttggaatac gatgccttgt catacacatg
    18241 aacgtcgtat ggaagcttat gtctactttg attatgccaa tgaagataca cgtgtgttcc
    18301 acatgatggg caaaccagat gaaacaaaac atttagttgt agacaatgaa caagccatta
    18361 tttcgccaag ctggtcgatc cattcaggtg taggtacaag caattactca ttcatctggg
    18421 caatgtgcgg agaaaatatc acctatacag acatggatat ggtgcaaatg gatcaattaa
    18481 aataagcata agggagagaa aatgatggaa tttagtatgg atatgtttcg tttagatgga
    18541 aaagtagcat tagtcaccgg tgctgtctat ggtattggat ttgagattgc acgctcttta
    18601 gctacagcag gagcaaaaat cgtatttaat aacttgaatc aagaatcagt tgatgaaggt
    18661 atagcgcatt ataaagaagc aggcatcgat gcaaaaggtt atgtctgcga tgtgaccgat
    18721 gaagaagcgg ttcaagcgat ggttcaacag atcaaagaag atgtaggttc agttgatatt
    18781 ctagtcaaca atgcagggat catcaaacgg acaccgatga tcgaaatgga tgcggcagac
    18841 ttccgccaag taatcgacgt ggatttaaat gccccattca tcgtttcaaa agcagttatt
    18901 ccggatatga tcgaaaaagg cggcggtaaa atcatcaata tctgctccat gatgagtgaa
    18961 ttaggacgcg aaacggtcag tgcttatgct gcggcaaaag gcggattaaa aatgctgacg
    19021 aaaaacatcg cttctgaata cggtcaatac aatatccaat gtaatggaat tggaccaggt
    19081 tatattgcta caccgcaaac tgctccatta cgcgaaacac aagaaaacgg tgaacgtcat
    19141 ccattcgatc aatttatcgt tggacgtacg ccagcagcac gctggggcga gccaatcgat
    19201 ttggctggac catctgtctt cttggcttct agcgcatctg atttcgttaa cggccatgtc
    19261 ttgtatgtgg acggaggcat acttgcctat atcggaaaac aaccttaaaa gagctttagt
    19321 acactgttta gtggagaggt tgtgaaatca gcggattgaa cgaataccga aggacagctg
    19381 attaaacatc atttattcga aaaaagaggc tgggacagaa gcatttaact ccaagaaata
    19441 agaaggaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg
    19501 agttgctttt acttccgccg tttgtacgtg tttagagcgt gagacaaaag tgttctttac
    19561 ttttgtccca cgctcttttc tgtgcgtctt ccatacttca ctaagtcgat ccctcttgct
    19621 tcgcaccaat ccttcactgg tccggaaaag atcagagatt gttggtgtag ggccgcggta
    19681 ttcgttgcgc cgaccatagt ataaagcaga cgaagctctt cttgccattg cttcatcagt
    19741 ataatagttt cttctacgcc gtgattcatc agatgcgtca gtacggttcc tgaagtaccc
    19801 acggcatttg cgccgagaca aagggcttta aatatgtcat aggcattgcg gatacctccg
    19861 gaggctagga tttccataga agtgtctgct tcatttgctt caagcaatga agagactgtc
    19921 gattgccccc agtcagctaa ataactcagt tcccgcttgg agcgacgggc gttttcgatc
    19981 tgggtgaagc ttgttccgct tcgaccgctg atatcaactg tatgaactcc taaagcagct
    20041 agatcattga gcgtttctct tgtcatgcca aaaccgactt cttttacgat gagggggacg
    20101 tcaatggctg tttgagtttc ttgaataagt actttccaat tggtaaaatc acggtctccc
    20161 tcaggcataa ccaactcttg aggtgcattc aaatgaatct gtaaggcatc tgcgtgaaac
    20221 aatcggatag cttctttggc tctttcgaca gaagtccctg caccgatgtt ggcaatgatt
    20281 ttcccgtgag gatattcttg tcgcataatc gtataagtat cggctaaaga agggactttc
    20341 aaggcagcgc tgactgaacc agtggcaatc atcaaatctg cttctcttgc gacgattgca
    20401 agatcttggt tgattttttt tgttttttcg cttccgccag tcatcgcatt gatataaaat
    20461 gggctgctta gtgtaaaccc aactgcctgt gtacttagat cgacatcaga aactgctatt
    20521 tgcggcaaag ggttatggat gatccgtacg aagtcaaatt cgtttttttg tttatcatga
    20581 aaggctttag ctagtgaaac atgttcgtct tttcgattca tcggttttcc tttctttgat
    20641 cgctatagac atgtaacggc agtggagtga tttctgcttt ttcccatgca ctcattaaag
    20701 gaagaatgcc agatttctgg tcaacaatta cgattccgca atcgccccca cctgcaccag
    20761 aagattttgc ggctccttca tactgttcag ctaaattaca caatttgttc aaagcaggcg
    20821 tttcgatgac gaccccagtg attgcagata aatcatgcag taattgtcgg ttttttcgaa
    20881 tcatctgttg gatcaacgtt acattatttt ctttaaaccc tttgatcatt tcgttgacac
    20941 attctgtact attttttaag aaaagctgat aagccaccat tttatcttct ctcgaacggt
    21001 gaacttgatc gaccaaatca gaagtagagg cagggctacc cgtccaacca atcaataaac
    21061 gtaaatcttc aggagcaatc aatggctcaa tggatagacc tggccaatcc agggcaagta
    21121 actcactgat agaatgctga gtttcttgtt cttggagcca aggatggtcg aaggttgaga
    21181 aagcgatcca gccaccataa cagctagcag cgatgtcgcc gcaggaacca ttatcttgaa
    21241 ctgctaaatt ggctagtgct gcaatcttga aaatctccaa ctgagataaa ttcaacgcgt
    21301 aaaaaacatt caaggctttg accgttgcaa cggtcacggc accgctagaa cccaatccat
    21361 atttccggcc atttgaactg tctaactcac ttgttacttt cagatcataa aaggataaaa
    21421 gaatgttttt ttcttgtgca tacttttcag tcaagcgaat agcagcaagg atataatgaa
    21481 aaggattttc ccgaatatct aaaactaatt ctccgttgcg tcttgtccaa cgtacaggca
    21541 tcccactata ttgtgcagat tggatacttc cgacttttcg tgcagattct acagtgactg
    21601 tcacgaattg atcgactgca gcgataactg ctggatggcc tgtttcaaca actgcatatt
    21661 ctccggcaat atagagtttg cctggtgcag atacttcaat cataaaaatc tccctttatt
    21721 caataatcgc aattcctgga ccagcatgtg cagtaattac ttgttctttg ctaaataatt
    21781 tggtaaatgt ttcttgtacc tcagataaat ggctgttttc cactaaaact ttgaaattag
    21841 ggccagcatc catcgtaaag tagcatggaa tgccttgctt tctcaattgt cgcaccgcat
    21901 ccatcgcttt taagctgtca ggagaccagt aagtgaatgg aggctgggca gctaaagtcg
    21961 ttccatgcat ttttagtccg tttctttcca tcgtttctcc gagaagttgg aaatcttttg
    22021 ttttgattgc ttgtttcaat tgatatagat ccccttcaac ggaatctaac cagccttgat
    22081 aaaaactgga tgtttcgact gtccgacgca tcccatttct gctggacact tcttttttct
    22141 ggtcgttgat caaaacgaaa accattgcta aatcgtcttc gaaagaatcg gaagggactg
    22201 gcttagcgta agaacttaag tcgtcatggc ctttttccca ttcgacgaaa ccaccgaaaa
    22261 tacttcggca agctgaacca gacccgcgtc gagcaaatct tgaaagagag agatcatcta
    22321 atccaagctt aagagcagta ttgcaagcgc ctgctaaagc agctagcccg ctggcagagg
    22381 aggctaatcc agctgcggta ggaacgaaat tctgactgat tactgaagct tttagtgaga
    22441 ggccggcttc tttacgaaaa aggtcaagaa actgactgac tttttttgtt gctttttcgt
    22501 cttgcaaggt gccatctaga taaaattcat ctaccatata gctatcagaa aagatgactt
    22561 ctgtctctgt ataaaatgca tccaatgtta acgaaaggct gttgttcatt ggaaggatga
    22621 gttcttcatt tttcttaccc caatatttga ttagagcaat gttggtatat gcgcgtgctt
    22681 tgcctttaaa cataggtatg tactcctaat ccttgtatcc aagtttcagc agcacccgca
    22741 tcttcaagtg cttgactgat ttcttgggct tttgtttttg tttgtgctaa tgcaatcata
    22801 cagccaccgc gtccaccgcc agttaatttg gcacccaatg ccccggtatc ttttgctgtt
    22861 tggatcaata gattaagaaa atcattgctg attgtcagct tttccagaag tgattgtgat
    22921 tcatccattg tctgtgccaa cgtttctggt gaattttcaa tgattgcttg ttttgcttgc
    22981 ttcgtcaagt atcctaattt ttggatcatt tgtccagttt catgaggctg ggtttcaaag
    23041 aggtgagcga catctttgac ggcttctctt gtttgtcctt tgattcctgt atcagcgaca
    23101 atcaaaaaag catcgatgtt caaagaaaag tagtcgaaag gatgcccttt tgtaaaataa
    23161 atcggctgca agctgctagt agcggctgca tcgattccac taggattacc atgggcgatt
    23221 ttttccgaaa gctggacatt ttctaatagt atttcacgag acaaaggaaa tgctaagtaa
    23281 tcataaaaag cacgagtgac tgctgtggcg actgcagcgc ttgatcccat tccacgttca
    23341 gcaggaatcg tgctttcaat ggtcagttgc aagttttttt ggatgtcatg ctgttttttt
    23401 aagtgaatga ataatttttt gatatttttt aatgcatgag gagcttcggc taaatttcct
    23461 gaatagtagg aagaaaccag ttgatcgtcc attgcatcca gctcttcaag gaatgcggtg
    23521 acttttgttg catagaaagg aaaggcaatc gctggttctc cataaacaac cgcgtgctcg
    23581 cccatcaata tgatctttcc gcttgattct ccttggccat agtttgccat aatctaactc
    23641 cttctcacgt aaaagtgatt tttaagcaaa aaataaagcg cccaaaacga gttgcgctgc
    23701 ttcacgaatt tttttgtcca ctaagggtct caaaaaacat tcctccttta ttttactaaa
    23761 accatctcag aagtaaaact aagaatacgt tttctaaaga aaaaacactt ttttcttaag
    23821 aattttttga ctttttttct tctttgacta gaaaaaaata caattgacac tactagatat
    23881 tgggggtata tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga
    23941 ggtaaatgag cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag
    24001 aaatgatggc tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag
    24061 tcttgccctt attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt
    24121 tgaataaacg ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc
    24181 gttcgattta tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag
    24241 aaacagaagt tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg
    24301 ctgcccataa aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt
    24361 tggagcgtat cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat
    24421 acgattatta caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa
    24481 gtccaaaaat ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag
    24541 aagaagtctt gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca
    24601 tttgatttac agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat
    24661 actggaaaca aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc
    24721 gtaatttttg gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag
    24781 aatatggcat tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg
    24841 tagagtacga ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa
    24901 aagtcgataa tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt
    24961 ttacatttac cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac
    25021 gaatgccaga catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt
    25081 ccttttttcc tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa
    25141 gagaaaaaac aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt
    25201 acttagttac aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta
    25261 tttatcgcaa gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac
    25321 gacaagcagt tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt
    25381 ataataattt gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc
    25441 tgatcttgac cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct
    25501 ttacccattc cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt
    25561 ggatgcttat tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag
    25621 ggcttgggcg atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg
    25681 agataaagcg attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg
    25741 aaaccagttc gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc
    25801 ataaaattcg ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact
    25861 actaaaggaa gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc
    25921 ggcaaaattc gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc
    25981 aatccactcc agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg
    26041 acgtaaaact gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt
    26101 tttggcatcg agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc
    26161 atcttctaaa taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat
    26221 ttttttcatg tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc
    26281 tagaaagtct gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt
    26341 gacaaaaaga ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg
    26401 cttctcgcct tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg
    26461 aagtacgcgc gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt
    26521 ttctttctct cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga
    26581 cggcattcgt gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa
    26641 gacaaaagca gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc
    26701 ccctggtgcg aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca
    26761 acaaaagaaa gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg
    26821 cttaagtcct acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt
    26881 cttggaaaag ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca
    26941 taaagaaatt ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac
    27001 agtggaacaa aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa
    27061 cgacagtaag taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa
    27121 agattttgag gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa
    27181 cgtttcaaac gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt
    27241 ttccacggca aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt
    27301 ggcgacttca aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca
    27361 gaatctgaag atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac
    27421 agtaactcgt aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa
    27481 acacacttta tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg
    27541 cgatcgtcgt caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc
    27601 agctcgtatg aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat
    27661 cgacatcaac cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc
    27721 attggctgac caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa
    27781 gaagataacg aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga
    27841 aacaggacaa taacgtataa gtctaaaata gcataattaa ggttttttaa ttgaaaactg
    27901 aacatttatg gtcacccttt aatgaaagcg ctaactacat aagttagaat gagtttgact
    27961 gatatattag tttgcttatc agaaaaacaa aatggacgga ggaataacat gagtgatatt
    28021 attattggta ttttgttagt tatctcattc tttttcatgg tctggtactg tgtaaaaggc
    28081 tacaacttga tggtcggctt tgccatcatg gctactgtat ggatgggatt agcgctagtc
    28141 ggaaacacgt tttcaccaaa cccggcgatg gaagggcaag gagtaattga tgtgttgacg
    28201 catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa tgtcttcttt
    28261 ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct gattcgaaag
    28321 gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg tattgtaacc
    28381 gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc agttattgtg
    28441 ttgccaatct tgatgtcatt aggtatttca gtaccggttg cactgttctc atttatgggc
    28501 tcgattatgg ctggaatctt tgctaatatc gtcaatttca aacaatatca aacgatttat
    28561 gctggtttca atccagcggc tgaaagctat acttataatg actatttcca aattggtatg
    28621 attgggatga ttgtaagctt agttgtggta ctaacggtcg ctaatatatc aatgaacaaa
    28681 aagaaacgct atgcaatggc agcaaacgta ccagctgaag gtggcgacgc accaatgatc
    28741 tcatggttag ccgtattatt accagtttta ggggtcgttc ttttagatct tccaatcatc
    28801 ttaggtttta ttctagcagg tatctgggca ttgctgttta ctggaaaatt gcgcggaggt
    28861 tataaagaaa tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt agatgttgcg
    28921 ccaatggttg gtttcttgat gaccttagcg atgttcaata attcagctgc gtatgcttct
    28981 ccttattttt cagctatttt tggagattgg ataccgcaat caccattagt cttggcaatc
    29041 gtatttgcta tattgacacc actaggattt ttccgcggtc caatgaatct agtcggttca
    29101 ggctcagcta ttttagctgt cgtattagct gttaatccaa cgatgtcgcc agcctttttg
    29161 ttcccgttat ttgcaatcac aacgattgct cctcagcatt tagatattac acaatcttgg
    29221 gttgcttggg gattaggtta tacaaaagta acttctcgag aatatatgaa gaaatccatc
    29281 ccaactggtt ggatcatcgg agcgatctta tgcctgatca cgttcttatt gtacgggaac
    29341 gcctagtaaa caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac
    29401 gttttgtgcg aatgggaatt gatgttggcg gtacacatac gaaagcagtc gcaatcgata
    29461 atgcgacgca cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg
    29521 gagtagccgc aggtgtcgtt cagtcttttc aaaattgttt gctggaaaat aatatcagtc
    29581 cagaagatgt tgtgtttgtt gcacattcta ccacacaagc gactaatgca ctgattgaag
    29641 gagacgtagc aaaagtcggc gtaatcggga tggcaaaagg tggattagaa ggttttttag
    29701 caaaacgaca aactagattg aacgatatcg atttagggaa taagaaaaaa atagagattg
    29761 tcaatgcatt cttacctgtc aaacacttaa atgtagaccg tgtttcagaa acgatttctt
    29821 ctttagaaag agaaagagcg gaggtcttgg tttcttctat ggcatttgga gtagataatg
    29881 gggaaccaga acgagttgtt tatgaagcag catcggtaaa atcgatccct acaacgatgg
    29941 cttctgacat cacaaaatta tatggattga caagaagaac tcggacagca gcaatcaatg
    30001 catctatttt accaaaaatg ctagatacgg caacctctac agaagattcc gtacgagaag
    30061 caggggtcaa tgtttctttg atgattatgc gcggagacgg cggagtaatg gaaatcaatg
    30121 aaatgaaaaa acgacctgtt ttaaccatgc tttccggtcc agcagcatca gtcatgggct
    30181 ctttgatgta cttgagagcg tctaatggtg tttattttga agtaggcgga acaacaacca
    30241 atatcggggt cattaaaaat ggacgtccag caatcgatta ttcaattgta ggtggccacc
    30301 caacatatat ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac
    30361 gggctaatca gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt gccggtctag
    30421 attatgcagt atttacggag acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc
    30481 ctaaagaagg agaccctgct gattacgtca aggtagtgat ggaagacgga gaagaagtga
    30541 ctattacaaa tacatgtgca gccaatgtcc ttggattagt gcaagaagaa catttttctt
    30601 atggaaatgt cccatctgca cgaaaagcca tccaagcatt agctgattat tgtcatacga
    30661 cggttgaaga catcgcagaa cagatcatgg aaaaatccta tgctaagatt gaaccggtga
    30721 ttttagaatt ggcagacaag tatcacttgg agaaagatca gatttctctt gtaggtgtag
    30781 ggggcggcgc tgcctcattg attacttact ttagtaataa aatgggtgtg aaatattcga
    30841 ttcctgaaaa cgccgaagtg atttcttcta ttggtgtagc acttgcaatg gtccgagatg
    30901 ttgtagaacg tatcatccct tcgccaagta aagaagatat acgatcgttg aaaaatgaag
    30961 cgatgaataa agcgattgaa tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa
    31021 tcgatccaca gacttcaaaa gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa
    31081 ccgatctaac caaggagatc acaacagaag aagcactaga gcttgctgca gaggatatgc
    31141 gattaaataa aaatgaagtt tgcttattgg aaaatacacc tttcttttat gtatgcggcg
    31201 aacagaatcg ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa ggatttatca
    31261 aggtccaacg tggacatgct tcatgtatga aaacaactgc agccaattat atgacagctg
    31321 tcgaacaact ttgggaagat atggcagttt atcaaactga attgattgcc cgaccagaat
    31381 tttatctctg cttaggagca agagtctctg attttacagc gacggatctg gaacaactac
    31441 agcttttgat ggatctggaa gtatctacta tggaaccgga ggaagaagtg atcgtcgttg
    31501 ccggaaatat caaacaaaca taagttagaa gagttactaa acgaattaca acagatcccc
    31561 gaagaaattt ggggattcta ccagtttcgg cgagatttat tttggaaaaa gattcctttg
    31621 tccaagcaga aaattcttat acaacaatca atcgattgtg gtatcgaaac ggcatgtagc
    31681 atcaaaaaaa agtacccttt tgccgatgta ggagaaattt gtgaacaaat ggctgttcca
    31741 atcgtttcct gtgaatcaga gcaaataaac gaacgaatca cttttgcaac ttatgcagaa
    31801 gatgaaggga ttcgattgat gacggagccg ttagaaaaat taaaatgttc aggactagct
    31861 tcgatctcta aagaaacagc gcaagctttg attattggac atgaactatt tcaccacatc
    31921 gaagcttctg ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct
    31981 ttttatacac atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat gagtttttca
    32041 aaagagatga atcaaagcga tttttcgcct tatgtattag aggcagtctt actttggcca
    32101 tataatgaaa ttcgtagcca agggatctta gaagaaataa aaaaaataga aaaaagatgt
    32161 acggagtatg atttagatta caaataaaga gactgtgaca aaagtcgtgt cacagtcctt
    32221 tttttccgaa taaacgatgt tctcctgtca actcctcggt cacaagtcaa aatattcaac
    32281 aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc
    32341 acgacctctt ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    32401 taaaaacata agaagctatt tttacttggc tgttcttatt acttgatgca gggcagcttt
    32461 ttcacaacct ctcaataaac ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa
    32521 acggcaaaat atgaggaact attttgccgt ttctttctct aatactcagg tcaaaacgct
    32581 gattccaaaa cctcttattt gatgggtctg tcctgaatat cttcaaaata atcggggtaa
    32641 ttatgaatca cgtattcttg ctcctcgata atcatatgta aatgcatcgt gcaaagaaat
    32701 ccgacttcat caaagttatg atcaatcatt gattgaagca ttgtctgatg ttcatctaat
    32761 actcgacccc agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg gttcaagtgc
    32821 gtactataac tttctagcca atcccaaatt tctcgttttc cagcaatttc ataacaagtt
    32881 cggtgaaaat cagtatccaa atcaaaaaaa gcttttttat tttgagcgat tactgcctga
    32941 tcggtttgtt ctagtatttt ttctagttta gcttgatctt gcttggtcat tttcgcactg
    33001 cattcttgca ggatcggttt ttccaacttt tcccgcgtat aacgggctaa tgaagcagat
    33061 cggatatcga tttttgaaac ataagtgccg gactgtggaa tggtttttac aagctcttga
    33121 ttgcgcagtt ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag ttgttcttct
    33181 aacactttat cggagatttt acttccaggt gctaactcgg aataaatgat ttgtttacgt
    33241 atagattcat aagcttgctg ttgcaaatta ttgataacca tttaaccttc ctttctatgc
    33301 agatataatc tattataaca taaatcgctt acaaatcgtg tgggcataaa aacacagtct
    33361 gcaaaaaaag ccctgatagg catgcctatt agagcctcga aaagaaaaat attgaatcaa
    33421 tctggtcgta cctcttggat cggtttgaca cgaccgccga ttcttttagc aaaagcttcg
    33481 gattcttttt tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg agtgcgacag
    33541 ccagttttat cgatgtaacc gtgcattact ttaacgacat acatggggat cacctgcttt
    33601 tttcttttca ttatacgaac tactcgacac aatacaagaa aaatgcttag acggtcttta
    33661 ttaagaattt acatcatcca aagaagcgta cgcttggaat aactgatcta actccggatt
    33721 gactttgctc agcttcacta atcgttgcga atcagaaagt aaaaaacagt gtttactact
    33781 gccagtagtt tttaattttt tgtcttcagt tcccacaatt tcgtagctaa aatccaaccg
    33841 tgtccccgta tacttttgga tagttggatg gatgctcact ttttctccgt aatggatcat
    33901 ttctttatag ctgcaattta cttccagaac ggggatgatg atcccagatt cttcgatttt
    33961 tttataggga aaccctagat gtaccaataa ggctacccga gcctcttcaa accagcggat
    34021 ataattgctg tgatgaataa tccccatttg atctgtttca taataaaaag gttctcgcac
    34081 ataacctgta aatttctcca tgactcatgc ctcgttttcc attgattttt tacaaaaata
    34141 attcatccat ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat attgttcttt
    34201 tgccagctgg tctccaatga aactatgaag ataaatagct gctgcaatcg tttggtcatt
    34261 ttgaggaaac tgggctaaaa atcctgtgat catcccagct aaagtgtcac ccatgccgcc
    34321 cgttgccata gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac cataaatttt
    34381 tgtctcatga cttttcaaaa cgagcacagc accgatttcc tgttgcttct ttttgttttc
    34441 tttatcccgt tgttcttgta tcggtagaga actcaaccgt tgccactcca tttgatgagg
    34501 tgtaagtaca gtttgttcgg gatagggtag caaaagatga ttagcagcaa gtaaacttaa
    34561 agcagagcca tcaatcacca gccattgttc ttttgattgg ttttctagta cttgtttgaa
    34621 acgttccaaa ctcttagaag aagtacccaa tccgggacca atcaagagta catctgcttg
    34681 ctttattacg gaaagaaact ggtcagtatc cgtccaaact acaatcattg cttctggcag
    34741 atgactgtga agagcactcc gatttttttc atcagttact acagtcgtga gacccgctcc
    34801 gctgtgtacg gcagctaaag cactcataat gattgctccg ccatattgtt cattgccgcc
    34861 aatcaaaacg atacgtccaa atgttccttt atgtgaaatt tttggacgag gtgtgatgac
    34921 tcttttcact agttgttcgt ttatcagcat ctaaaaacct ccttttactc attatagcgt
    34981 atattttttt tagagaaaaa cagataagaa aaggaattgt cagatttttt gaactatttt
    35041 tttctgtaat ttgtggtatc atgaacacga agacttaaat aagtggagga ttaactttat
    35101 gacaattgat tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg aagacttgaa
    35161 aaatctttta cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag atgcaccatt
    35221 tggacctgga cctcgcgacg cattgaaaca tatgttagct tatggcgaac gtgatggatt
    35281 taaagtaaaa aatgtcgata actatgcagg gcatattgat cttggcgaag gagacgaaac
    35341 gctagggata tttggacaca tggatgtcgt tcctgcagga gatggctggg acacagatcc
    35401 ttatgaacca gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg atgacaaagg
    35461 tccaagtatg gcagcttact atgcgatgaa gatcattaaa gaacttgact tgaaactatc
    35521 aaaaaaagtt cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg atatggctta
    35581 ttatttcgaa catgaagaag aaccagattt tggtttctca ccagatgctg aattcccaat
    35641 catcaatggt gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg acaatgcagg
    35701 agattatgtc ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc caggaacagc
    35761 aactgcagca cttcaagtac caagtgcaga tgcagctatc gcgatggaag aagcatttta
    35821 ccaattcatc gaagcaaatc cagtaagcgg aacgattgaa gcggacaata cgtatgtaaa
    35881 aatcgaatta gttggtaaag gcgcacatgg tgcaagccca caatctggta tcaatgcagg
    35941 ttctttcttg gcattgttct tagataacta tgaattccta ggatctgcta aacaatttat
    36001 ccatgtagca gctgcttatg tccatgaaga tttttatgga gaaaaactag gagtcgctta
    36061 tgaagatgaa aaaatgggca aattgacaat gaatgctgga ctatttgctt ttgaagaaaa
    36121 tggtacagag gaagcgaact tcatcaatat gaatttccgt tttccaaaag gtgtaacagt
    36181 agacggattg caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa cagttactcg
    36241 aggtgctcgc gtaatggaac cacattacgt accaatggat gatccattgg tggctacatt
    36301 attgcaagtc tatgaagacc acactggaga aaaaggctac gaacaaatca tcggtggcgg
    36361 aacttacgga cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc caggatatac
    36421 tgatacaatg catcaagcaa acgaatttat gagtcttgat gacttgttca gagcaacagc
    36481 aatctatgca gatgccatct atcgtttggc gaaataatta attaaaaaga ggatcaagaa
    36541 ttgcaaatta tttttgcagt cttgatcctt tttttacttt ctactgtggt attcatgata
    36601 aacatgatat agattcaatt catattcttt cttagcgttc gtgacgactg tatccaaaat
    36661 caaccctgtt actaatgaaa gaagtgctaa agtcatcaat ccagtactta aaatcgcaga
    36721 agggatgcgt gtaataaagc ttgtcagcat atattcgcga atcacaggaa tccccgtgac
    36781 caatccaaat aaaaagaaga aaatggtcca aatcccaaag aaaagcaaag gcttataatc
    36841 tttaaacatt ttaataatca tcatgatcac tttgaatccg tcagagaatg tattcaattt
    36901 tgattcgctg ccttcaggac gatcacgata atcgattggg atttcaacga acttgaattt
    36961 tttatctaaa gcatggatcg tcaattccgt ttcgatttga aaaccagaac tcataatagg
    37021 aaaacttttg acaaagatac gattgaatcc tcgatagccg gtcatgacat caagaatttt
    37081 tgttttatac attctagtaa ttgtatttct aacaaggtta tttccaaaat cgtggaaagg
    37141 tctcttattc tcatcaaagt aggttccatt tgataggcga tcaccaatga ccatatctgc
    37201 ttcaccagaa cgtaatttct cgagcaaacc atgtacagct tctgcaggat aagtatcatc
    37261 gccatctacc attaaataat agtcggcatc gatatcaaag aacatttggc ggatcacatt
    37321 tccttttcct tgacgaggtt ctttttttac gattgctcca ccttgaacgg ccaattcgta
    37381 tgttcgatcg gtcgaattat tatcatagac ataaatatct gcttctggta attctcgttt
    37441 aaagtctgca atgactgttg aaattgttgc ttcctcattg taacaaggaa tcagaacagc
    37501 aattttttct tgttgttcca tttttcactc tccttaacta tagaaaacaa aagctaaatt
    37561 aaaaatacct cataatcaaa ggaaatgcta gtttttcccg ctaaatatca tttatttaaa
    37621 aagaaaatac tggaatatat gtttggaaag ctctatctaa aaaaccaccc actacaaaag
    37681 aaggacatga tacctctctt ttgaatagtg ggtgcgtatt cgttctctca cttttttgac
    37741 gtattaatct gttgcacctt tttttcttga aatttctaat acggttcctg tatgagcatc
    37801 tgctaaaaat tcataataaa cagtttcgcc atcttctatt cgagaaagac ctccccgata
    37861 agcactcgac cgaacggcga atttcctaat aggttccttt tcaaaagaga tccaagaacc
    37921 ttcgattgca ccttctttta gaaaagatga cttgatattt tcaagtactt ggtctgctga
    37981 caatactttt ttgcgatgat accaaatcgt tgaagctacc ccgctcaata atcctagacc
    38041 gattccaact gctaacccgc ctttaaaata gcttagttcg ctttcttcat acatgtggac
    38101 atacctcctt cttctcactt gttactcctt gtgcttattt taccacactc tcatattttc
    38161 tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat cggtctgcag
    38221 aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag tctgaaattt
    38281 agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa cagaattaca
    38341 aggaacaagc ggttttgaac aagatgttcg cgcttatatg gaaagtcata tggaaccgct
    38401 ggtagatgag ttacagttag atggtttggg cggcattttt ggtctgcgcc atcatgaaga
    38461 agcggatgca ccccgtgtaa tggtcgcagc acatatggat gaagtcggat tcatgctgac
    38521 acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggctgga atccttacgt
    38581 tgtttctgct cagcgtttta cacttaaaac ctctactggt aagaattatc catgtatttc
    38641 ttcttccgtt cccccacatt tattgcgagg aacaagcgga caaaaatcag tagaagtaac
    38701 cgatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt acggtgtcct
    38761 tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg gaaagaatat
    38821 tatcagtaaa tcatgggaca accgctatgg ctgtacaatg gtattagaag ctttagaagc
    38881 gttgcaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc aggaagaagt
    38941 aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt tctttgcagt
    39001 agactgctct gcagcagatg atacagtgac gaaaaatggc acattcggac atttaggaga
    39061 aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt tgcgcgagta
    39121 cttgctggat atcgctgaaa caaacaatat cccttatcaa tactttgttt caaaaggcgg
    39181 tacagatgca ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag tgattggcgt
    39241 agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt ttgaagctgc
    39301 acgagaaatg ttgattcaaa cactgaaagg tttggataaa tcaacagtaa atacgatcgt
    39361 atacggaaaa taatgcgata gtgaaactat aaaatctggc aaaagattgc tttttgccag
    39421 atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg tataatttaa
    39481 ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa tagtttagaa
    39541 gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc cggctggtgc
    39601 ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt ttctgattgg
    39661 caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg gaacattttt
    39721 ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct agtgaataaa
    39781 gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa aggctgacat
    39841 ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg tatcgacggt
    39901 agtgaacaag cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg taataatggg
    39961 aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg ctatgcacct
    40021 attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat cgaagaatgt
    40081 aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc atatggttct
    40141 gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct aatcatggtt
    40201 gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc cagctacgtg
    40261 atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca gccaagtaat
    40321 taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta aagggactgt
    40381 gacaaaagtc ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaggcttctt
    40441 cggaaataaa gcatgaaaaa aatttcatgc agtgaatgca attttttgtt aagataaatc
    40501 tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga
    40561 gacaccttga tggttattat agccgacgat aaaggaacag aaaacactgt tgaacgcaaa
    40621 tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat
    40681 atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga tgaggaatta
    40741 gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttga agctgatcat
    40801 gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga ttctgaccat
    40861 ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt ttgcggtgca
    40921 cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat gatgccagat
    40981 ggaatgatga tctggccagg aactttgcga ggggtagata gctatggaat gatctgttcc
    41041 gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt attgcctgag
    41101 gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa
    41161 cctctttttt attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc
    41221 aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct
    41281 cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca
    41341 tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg tgtagctgtt
    41401 tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct
    41461 tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc
    41521 acttttccgt ctttttccag ttgattgatg atattcacta catcgttact tggaatagca
    41581 aatcccattc cttctacgct tacattagaa gtagattcag aagtacttgc gatcttactt
    41641 gagttgatac cgataacttg tccttcgata ttaactaaag gaccaccaga atttccaggg
    41701 ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga ttcattcgta
    41761 cttgtcactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt tgcatattca
    41821 gatcctagtg gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc acttgagtca
    41881 ccaaaagagg caactgtttc gactttgtct gaattgatct tcaacaccgc taaatctgaa
    41941 taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat caagacttca
    42001 agaccttgct ggccatctac tacgtgatta ttcgtgacta cataagcagt attcccgtct
    42061 tttttgtaga tcacgccgct tccttcacta taagcttcca aattactatc atctgagctg
    42121 ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt ttgactttgt
    42181 aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa
    42241 tctacattga ctttgacatt ttccacaact gtttcaccgg cagaattctg attgccgctt
    42301 gtagcagtag aactattgtt gccagtgccc atcgctgcat aaaaaatgcc agctgtcagt
    42361 agtcctccaa cgatccctcc aacaagcccc agacctaact tacgccagag gctattattt
    42421 ttactttttt tcatctttgg tgtcacattt tttctatcca tgtgttcctc ctccaattac
    42481 attcatttat ctactgttag tatagtctta tttgttgact ttagtctact ttgagactgt
    42541 gaacttttta tgaaaaactt taggaaaaat cattgttttc agatgaaaat atttgatttt
    42601 ctttttccac agatactgtg taaaactacg cggataggat tggaaaaata agggataaaa
    42661 catatccaca gcctgtgtat aaagtggata aattcttata aaacgtatgt ttgcctaatg
    42721 aatgtgatga aataaggtaa aataacactg ttttctgtgg ataaacctgt ggatgttgtg
    42781 cataactgtt ggggaaatct ttttaacaaa aatagccaaa ataaacatat agacgaaaaa
    42841 attgattata ataaacaaag tgagcatgaa gaccaaagga gagaaaacat gaatatcaaa
    42901 attatctcag taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa tgagtatgtc
    42961 aaacggctga atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga aaaagccccg
    43021 gagaatttaa gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga acggatctta
    43081 gcgaaaatca aagaacaaga atacgtctat gccttagcaa ttgaagggaa aaatcctaca
    43141 agtgaatctt ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag ccaccttgtt
    43201 tttgttatcg gcggctcttt aggactcagc gaagccgtca tgaaaagaag caatgctcag
    43261 atctcttttg gaaaaatgac gtatccacat caattgatgc gtctgatcct tgtcgagcaa
    43321 atctaccgtg ccttccggat caatgcgggg gctccatacc acaaataatg atatttttta
    43381 agagaaaaaa ttatccctat tttaaataat agcaataggt agtggtaggg agaaatttat
    43441 aaataacttt tttatttgct ttggtagcca agtaagttgc aaaagaaaaa tagatactgt
    43501 taaaaagata atgtagacat agaaacaaat acgtaaaatt ttagttagaa taatggcaag
    43561 gagaaaaaac atgatagatg aaaaaaagtg tttaaaaaat ctattagagg atatggatga
    43621 tattgaagaa aactatctaa atggactggg aaaacaaata tatttcagat acttaagcta
    43681 tctaaaaatg aaataagaca tagtaatttt ttagcttttt tgtttgctcc aaatgaaacc
    43741 cacaatttat ctgatgagtt ttttaaaatg tttttgaaaa ggtatataga ttctaatgat
    43801 gataccaaag ctgctatcaa ttattttgat gctttactta atagctatga agatttaatt
    43861 gtttacagag aaaataagaa tattgatatt ttattggtat cggaaaaaaa taaaatagtg
    43921 gtgtgcatcg aaaataaaat tctgtcttcg gagtctggag ggcaacttaa taaataccaa
    43981 atatatatag aacgcaatta ttctaactat aaaaaaattt ttgttttttt aactccagat
    44041 ggaaatgaac ccacaaatcc tagttggggc ataattactt acaagaatat cgttgaagag
    44101 agtgtaaaat attttgtgta aatagaaaaa aggaagtccc ttctgtagaa tagagttacc
    44161 acaacacatt cacagaaaag aggacttcca tatgaacgat tttactacag aaattatgga
    44221 aatactaatc aataaaggtg atttggatga tttgtttcgt cgtcacctag aattggctat
    44281 taacgcttta ttacaagctg aattaacggt atttcttgac tatgaaaagt atgatagagc
    44341 tggatttaat tcaggtaatt cccgtaatgg gaattattcg cgctctttta aaacagagta
    44401 cggtgaattg aatttagtaa tacctagaga taggaatgga gacttttctc aacaaacttt
    44461 acctgcttat aaaagaacca atgactcttt agaaaccact attattcagc tagttcaaaa
    44521 aggaattact atgtccgaaa tctctgatct aatcgaaaaa atgtatggtc attattacaa
    44581 accacaaact atttcaaata tgagtaaaat tgtgtctgaa gatgtttcgg catttaaaga
    44641 aagaacttta gaagctaaat actcagtcat ttttatggat gctactcata ttcccttaaa
    44701 aagacaaacc gtatcaaaag aagctgtcta tatagtaatt ggtatcaggt tggatggaac
    44761 taaagaggtt ctaggattta ctatagctcc aactgaatct gcttatgttt ggaaagagat
    44821 tcttcaagat ctaaaagacc gtggtttaga agaggttttg ttagtcgtaa cagatggttt
    44881 aagtggtatt gacgatagta ttcatagtgt ttatccaaat gctcaatttc aacaatgttg
    44941 tgtccatata tctagaaata tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg
    45001 tagcgatttc aaattagttt a
//
LOCUS       DACCVU010000010        42803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCVU010000010 DACCVU010000000
VERSION     DACCVU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.010"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            46..291
                     /locus_tag="IVW29_000473"
     CDS             46..291
                     /locus_tag="IVW29_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837979.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            384..830
                     /locus_tag="IVW29_000474"
     CDS             384..830
                     /locus_tag="IVW29_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7837980.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(889..1350)
                     /gene="mscL"
                     /locus_tag="IVW29_000475"
     CDS             complement(889..1350)
                     /gene="mscL"
                     /locus_tag="IVW29_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7837981.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1602..2870
                     /locus_tag="IVW29_000476"
     CDS             1602..2870
                     /locus_tag="IVW29_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7837982.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2863..4149
                     /locus_tag="IVW29_000477"
     CDS             2863..4149
                     /locus_tag="IVW29_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7837983.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4927..5811
                     /locus_tag="IVW29_000478"
     CDS             4927..5811
                     /locus_tag="IVW29_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7837984.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            6020..6598
                     /gene="pgsA"
                     /locus_tag="IVW29_000479"
     CDS             6020..6598
                     /gene="pgsA"
                     /locus_tag="IVW29_000479"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7837985.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6838..8079
                     /locus_tag="IVW29_000480"
     CDS             6838..8079
                     /locus_tag="IVW29_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7837986.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            8188..9237
                     /gene="recA"
                     /locus_tag="IVW29_000481"
     CDS             8188..9237
                     /gene="recA"
                     /locus_tag="IVW29_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7837987.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9648..11204
                     /gene="rny"
                     /locus_tag="IVW29_000482"
     CDS             9648..11204
                     /gene="rny"
                     /locus_tag="IVW29_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7837988.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(11283..12089)
                     /gene="proC"
                     /locus_tag="IVW29_000483"
     CDS             complement(11283..12089)
                     /gene="proC"
                     /locus_tag="IVW29_000483"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7837989.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            12230..14803
                     /gene="mprF"
                     /locus_tag="IVW29_000484"
     CDS             12230..14803
                     /gene="mprF"
                     /locus_tag="IVW29_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7837990.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14957..15808
                     /locus_tag="IVW29_000485"
     CDS             14957..15808
                     /locus_tag="IVW29_000485"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7837991.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            16053..16991
                     /locus_tag="IVW29_000486"
     CDS             16053..16991
                     /locus_tag="IVW29_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7837992.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            17014..17694
                     /locus_tag="IVW29_000487"
     CDS             17014..17694
                     /locus_tag="IVW29_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7837993.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17667..18491
                     /locus_tag="IVW29_000488"
     CDS             17667..18491
                     /locus_tag="IVW29_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7837994.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18658..19476
                     /gene="purR"
                     /locus_tag="IVW29_000489"
     CDS             18658..19476
                     /gene="purR"
                     /locus_tag="IVW29_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7837995.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19979..21352
                     /gene="glmU"
                     /locus_tag="IVW29_000490"
     CDS             19979..21352
                     /gene="glmU"
                     /locus_tag="IVW29_000490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7837996.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21446..22417
                     /locus_tag="IVW29_000491"
     CDS             21446..22417
                     /locus_tag="IVW29_000491"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7837997.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22423..22525
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22591..23160
                     /gene="thiT"
                     /locus_tag="IVW29_000492"
     CDS             22591..23160
                     /gene="thiT"
                     /locus_tag="IVW29_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7837998.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(23241..24014)
                     /locus_tag="IVW29_000493"
     CDS             complement(23241..24014)
                     /locus_tag="IVW29_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7837999.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            24281..25981
                     /locus_tag="IVW29_000494"
     CDS             24281..25981
                     /locus_tag="IVW29_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7838000.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25951..26169
                     /locus_tag="IVW29_000495"
     CDS             25951..26169
                     /locus_tag="IVW29_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838001.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            26189..27079
                     /locus_tag="IVW29_000496"
     CDS             26189..27079
                     /locus_tag="IVW29_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7838002.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            27097..27381
                     /locus_tag="IVW29_000497"
     CDS             27097..27381
                     /locus_tag="IVW29_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838003.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27868..28482
                     /gene="gmk"
                     /locus_tag="IVW29_000498"
     CDS             27868..28482
                     /gene="gmk"
                     /locus_tag="IVW29_000498"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7838004.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28487..28792
                     /locus_tag="IVW29_000499"
     CDS             28487..28792
                     /locus_tag="IVW29_000499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7838005.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            29045..31453
                     /gene="priA"
                     /locus_tag="IVW29_000500"
     CDS             29045..31453
                     /gene="priA"
                     /locus_tag="IVW29_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7838006.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31468..31959
                     /gene="def"
                     /locus_tag="IVW29_000501"
     CDS             31468..31959
                     /gene="def"
                     /locus_tag="IVW29_000501"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7838007.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31952..32890
                     /locus_tag="IVW29_000502"
     CDS             31952..32890
                     /locus_tag="IVW29_000502"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7838008.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32890..34248
                     /gene="rsmB"
                     /locus_tag="IVW29_000503"
     CDS             32890..34248
                     /gene="rsmB"
                     /locus_tag="IVW29_000503"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7838009.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            34261..35001
                     /locus_tag="IVW29_000504"
     CDS             34261..35001
                     /locus_tag="IVW29_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7838010.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34998..37067
                     /gene="pknB"
                     /locus_tag="IVW29_000505"
     CDS             34998..37067
                     /gene="pknB"
                     /locus_tag="IVW29_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7838011.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            37232..38131
                     /gene="rsgA"
                     /locus_tag="IVW29_000506"
     CDS             37232..38131
                     /gene="rsgA"
                     /locus_tag="IVW29_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7838012.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            38144..38794
                     /locus_tag="IVW29_000507"
     CDS             38144..38794
                     /locus_tag="IVW29_000507"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7838013.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            38795..39433
                     /locus_tag="IVW29_000508"
     CDS             38795..39433
                     /locus_tag="IVW29_000508"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7838014.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            39588..40442
                     /gene="mreC"
                     /locus_tag="IVW29_000509"
     CDS             39588..40442
                     /gene="mreC"
                     /locus_tag="IVW29_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7838015.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            40446..40955
                     /gene="mreD"
                     /locus_tag="IVW29_000510"
     CDS             40446..40955
                     /gene="mreD"
                     /locus_tag="IVW29_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7838016.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            41210..42784
                     /locus_tag="IVW29_000511"
     CDS             41210..42784
                     /locus_tag="IVW29_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838017.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        14120 a         7636 c         9257 g        11790 t
ORIGIN      
        1 aaattttaaa aatatgtggt atagttgata gtagaggtga actgtatgcc aacaacttta
       61 gcttcaatca agaaagattt agaaggtcgt atcggcagcc caatcatgtt ggttgctcag
      121 acaggaagaa agcgtcaaac agaacgccgc ggcgttttaa cagaaaccta tccttcagta
      181 ttcgttgttg atttggatcc tgaagaaaat tcttttgaac gagtatctta cagctattca
      241 gatgtactta ctcggacagt tgagatcgaa tttttaactg aagcagttta aaaagcaaac
      301 gagcctgaga ttcgtaaggt tcgtttgctt ttttgtgtgc gaatcggtat gctaagaata
      361 aaaaagcagg gggcgtttgc ttagtggaat atcaggtaaa gaaatttgaa gaattgtcaa
      421 cagcagagtt ttacgcgatc gtcaaagaac gaatcgcagt ttttgttgtg gaacaacagt
      481 gtccttatca agaagttgat gacattgatg gagaagcttg gcatacgttt tttcaagata
      541 ctgatgggaa gatccttgcc tacacgcgag tatatgaaga agcggagagt atccattttg
      601 gacgtgtcct tgttcatcag gacaaccgta aagatggttt aggaagaaag attgttcaag
      661 aaacgattaa catgatccag aaaaattttc cagaaaaacc aatcgtgatc ggcgcacaag
      721 aatacctaca agctttttat gagtcgtttg gattcaaggc aatctcagat gtgtatttgg
      781 aagatgatat tccacatatc gacatgaaaa aggaacaagt agcaagttaa gaatctaaaa
      841 aaagaaagcc gacactgaaa atcggctttc ttttttttag attctttatt atttagaagt
      901 atcggaaaga tttgtttgct ctactggatg tgtattgtct tctgccactt gtttggcaag
      961 aagatcgcgt atgtcactaa gataatcttc tgcagtagga gtttcttcca cttccacttg
     1021 ctcttctttg gagcgtaaat tcttcgcacg gtttgccgct ttgacaacta agaataagac
     1081 aaaaccagtg atcagaaatg taatgatagc actaataacg ttaccaaacg caaatgtgac
     1141 gccttttgtc ggcgaccagt caagaattga aagtgctcct tctaaatcag aagtacctgt
     1201 gatcattgcg acaaaccaac ctattagtgg tgtgatcaag ccttgcacca cttggtttac
     1261 gatgcttgta aacgcactac caataacaac gccgacagca agatccaaga catctccacg
     1321 catgatgaac tctttgaatt ctttgatcat gaatgaccct cccttctttt atttgtcttt
     1381 agtatatcga aaaataaaaa ataaaaaaac aatatacgct tttagccgtc tgtttctttt
     1441 gataaaaaac atctatgcta tactataaag tgtaacatca tttagaaaaa acggcgaatt
     1501 gcctgaactt ataaatttca agcagaggaa taagtaaaaa aggcaggttg acccattaaa
     1561 agagtaagcg ggcaatcaac tgtgaaaagg agcatataaa tatggtgtat caattggcag
     1621 aaggcgtgaa tcttcatgtc ttaccaacaa aacaatacaa aacgatccgg atttttatcc
     1681 gttttactgc gcgtctgcaa caagaagtca tcacgaaacg ttcacttctc agcagcatgt
     1741 tggaaacaaa tagtttgaat tattcggatc agacaaagtt aagtgcgcaa cttgcagagc
     1801 tttatggcgc aagctttgga ttatcagtta gaaaaaaagg aaatttacat tggctaaacg
     1861 cagggatcag ttttgtgaat ggtgaatatg tcaatgatcc aaaccttttt tctcaagcgg
     1921 tagatttttt aaaagaggtt ttattttatc caaatattaa aaatcatcag tttgatcagt
     1981 tgacgtttga cttggaaaag aacaatctgc gtctatattt ggaaagtttg aaggaagata
     2041 aacaaacttt tgcttcctat gccttgcaag aactttattt tgaaaacagc ccggaacaaa
     2101 agatcccagg gttgggcgta gtcgaagatt tggacaagat cactgctagt tcgctagcgt
     2161 cctactatca agaaatgatg gcaaatgatc aaatcgacat ttttgttgtc ggcgatgttg
     2221 atccggacaa agcagctgaa gcagttgggc aattaccatt tgagtcgcgg gaaacagttt
     2281 atccagatct gttttatacc caacctcaag tgaatatcgt gaaggaacga caagtacgtg
     2341 agccaatcgt tcaagcaaag ctgaacctag cttatcagac aaatgtgtat tacgatgaac
     2401 cagaacgttt tgcgctaatg gtctttaacg gtttatttgg cggttttcct cactctaaac
     2461 ttttcatgaa tgtcagagag aaagaaagtc tagcttatta tgcctctagt agcgtggata
     2521 cgttccgagg attcatgagt gtccaaaccg gtatcgatga aaagaaccga aatcaagtac
     2581 ttcatctgat tcacgagcaa ttagaaagtt tgcgaaatgg tgaaataaca gagttggaac
     2641 tgtctcagac aaaagcaatg ctgagaaacc aatatctgct ttctctagac tctccgcaag
     2701 cggcaatcga agcttctttt ttggacagct ggctgccgga aacaaaatta tctgatgaag
     2761 aatggttgaa acgtatggag agcgtaacga tcaaagagat acaacaagta gctgagcaaa
     2821 tcgaattgca ggcaatcttc tttttagcag gaggaaatgc aaatgaataa aacagaatat
     2881 gagcaaatca atgaaacact ttatcatgaa gtactgccaa acggtttgac cgtgtatttg
     2941 ttgcctaaaa atgactatca caagacatat ggtttgttca gtacgaatta tggttcgatc
     3001 gataacgaat ttattcccta tggtgaaaaa gaaaaggtga aagttccaga tggaattgcc
     3061 cattttttag aacataagtt gtttgaaaaa gaagatggtg atgtctttca attatttggg
     3121 aaacaaggag cttccgccaa tgctttcaca agttttacga aaaccagcta tctgttttct
     3181 acgacggatc aggtagaaaa gaatctgacg actctgatcg attttgtaca agcaccttac
     3241 ttcacagaag aaactgtaaa caaggaaaaa ggaatcatcg gtcaggaaat ccaaatgtat
     3301 gaagacgacc ctaactggcg gatgtttttt ggcatactaa ataacttgta tcctacacac
     3361 ccattgcata tcgatattgc gggcacggtt gaaagtatcg acaaaatcac agctcaagac
     3421 ttgtatacgt gctatcggac gttttatcag ccaagcaata tggttttatt tgtagttggt
     3481 aagatggagc ctgagaaatt gatgaaactt attcgggaaa accaagaagc aaaaaatttc
     3541 cctccaaagc aagaaattgt acgttatttc cctgaaaaca caaaagaaat catcaaacaa
     3601 agtgcattgg aagcagccat tacacgagat aaatttgtac ttggtattaa aggattagac
     3661 acattgccgc aagaaggaac agaactgtta cgctataaaa cagcaatcaa tctgttgttc
     3721 caaatgattt taggcaatac ttctcgaaac tatctagcga tgtacaatca aggaattatc
     3781 gatgacagct ttggttttga attcagcttg gatcgtgaat ttcactttgc ggacttcagc
     3841 ggagatacgg acgaaccaga gaaagcagca gaaaaagtca aagaaatcat tcttggattc
     3901 gctgatgatc cggaagtgtc agaaacaaat ctcgatttgc tgaagaagaa aatgcttgga
     3961 caatatttcc aatcattaaa ttcgatcgaa tatattgcca atcaatttac gcaaagttta
     4021 tttggcgatc gaactttatt tgatcttcct gaaatcatcg attcgatcca aatgaaagac
     4081 gtactggcag ctggggaagc atttataagt gaagaggcct tcagcgagtt ttacatgaga
     4141 ccaaaataaa ccagtatttt atgaggaaaa gtcttaaatt tcgttttaga cttgtagtag
     4201 gaacctcctc caaaatcagc ttttatggta ttcttagttg tgttggaaaa attagtgaga
     4261 aaataaaaca aatcatgctc tcttttaatc aacttatatt aaaataaaat caaacgaagt
     4321 aatcaaagag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa
     4381 ttgcttctca aatttttgtg atatttcggc ttattgccga agaagctact tctggaacac
     4441 cgtgtaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     4501 aaacagcttc ttaatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga
     4561 acaccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacata
     4621 cggaaaaata tctccttata ttttgccggt ttttgagcta ataccgagga acagctgatt
     4681 gaacaccgtt taagaaagag gtcgtgagcc tgccgtttaa ctccgagtca caaggaacaa
     4741 gttcaaaaaa cagcttctta tatttattgg atattgtgac ttgtgaccga ggagttggca
     4801 ggagaacaat gtttattcgg aaaaaaggag ctgtgacatg acttttgtca cagcacccaa
     4861 acataacgtc tttcgttgca acagcagatg cttcggagaa tcaaacagac tggagaagat
     4921 gaacgagtgg cttttgaaac catcggagaa aaattacggc aagcacgcct gaataaaaaa
     4981 atatctttag atgaattaca gcagattaca aaaattcaaa aacgttattt ggaagcaatc
     5041 gacaatgatg attttgacca attgcctggt aaattttacg tgcgggcatt tattcggcaa
     5101 tacgctgaag ctgtaggaga agatggagac catttagtcg atgtttttga tgggaaaagc
     5161 aaattgacag gatcggtccc tgaacgtccg cagcctgaaa cagtaagtac ttctagaaaa
     5221 gcaatgcatc aggaagaatc gcatccaagt cgcttttgga caagtcttcc tgtcatttta
     5281 ttaggattgg ttgctttggc gattgtcgtt gtcgtgggct atatgacttg gcaagatcgt
     5341 caagctgatc caataatcgg agacacagct tcttcgctta aagtagaagg atcagtcgag
     5401 gagtcctctt cctcagagca aagcacgcag accaaagagt cttctacagt agaatcaaca
     5461 acaaaatcaa gtgaagaaaa gaaaatggca atagctacag gagaagattc tggcagtgcc
     5521 atcgcagtaa atatcacaga cgctaccaag cctgtaaccc ttgaatttac tgctgtcaat
     5581 cgtgtatggt taggtgtgct agtagacaat gcctatgttt atcaagggac actggcagca
     5641 aatgaaacac agagtaccgt gttaccggaa acagcaacaa atgcgaccat cacgattggt
     5701 gcagcaagca atgcaacgat caaagcgaat ggagaatccg taccagttaa tccaggggaa
     5761 aacaatcaga gtccaaaaaa tgtgaacctt actcttcaat acgcagaatg atcgtataaa
     5821 ttagtttacg catctgttta cttgtaggta taaaaaagaa ggatcgtgac aaatctgtgt
     5881 cgcggtcctt cttcgttaat tcaaaaagat caatttcaat aaaaatactc ctttgaataa
     5941 attcatttgg aaaagtaaat aatatcggtg gaaacttcgc tgatagtcac gtagaatagg
     6001 gaagaaagga gtcgatagtg tgaatttacc aaataaatta actgtactta gaatttgtat
     6061 gatcccgatt ttcattttgg ttgtaggttt acctttagat tggggacaat ttaacgtagg
     6121 agacaccaca ctggctgtca cacagctagt aggtgcagtt atttttgctt tagctagtat
     6181 cacagactgg ttagatggaa aaattgcccg tgcgcgaggc cttgtcacta attttggcaa
     6241 gtttgcggac ccgttggcag ataaaatgct agttatgact gcttttatca tgttggttga
     6301 ccaaggaaag gcccctggtt gggtagtagc gatcattgtc tgtcgtgaat tagccgtcac
     6361 aggcttgcgt ttactacttg tcgaacatgg agaagtcatg gctgcagctt ggccgggaaa
     6421 agtcaagacg gctacacaaa tggttgctat tatcttgctg tttatcaata atatcccatt
     6481 cagcctgatc ggtattccag tagatcaaat catgctttat gcttgtctga tctttactat
     6541 ctattcaggt gtggattact tcatcaaaaa cagcggtgtg ttcaaaggat caatgtaagt
     6601 atcgtttagt ggagaggttg tgagcctgcc gtctaactct gagtcacaag ggataatttc
     6661 aaaaaacagc ttttcatgtt ttatgtatat tgtgacttat gaccgaggtt ttggcaggag
     6721 aacactgttt atttggaaca aggagctgtg acaagatttt tgtcacagct ccttgccctc
     6781 tttttaaata aaaaaatata aaaatacagc gtatactata taagggagcg ggaagtaatg
     6841 aaagcagaaa taatagctgt cggaacagaa cttttactag gacaagtcgt gaatacgaat
     6901 gccactttct tgtcagaaca gctagctgat ctaggtatcg aagtttatta tcaaacagtt
     6961 gtaggagata atcagcagcg tttagaagag ttgattgctc tagctgaaac aagaagtgaa
     7021 ctcatactct tatgtggcgg attaggaccg acagaagatg acctgacaaa agaagcaact
     7081 gcggctcacc tcggtaagtc tctcatacaa aatacggaag gatacaaaaa attattggca
     7141 tattttgaaa caacccatcg aaaaatgacc aaaaacaatc tgcaacagtc ccagattatc
     7201 gaaggcgggg ttcctctacc aaatcgtaca gggcttgctt tagggacttt ttatcaaaca
     7261 gatacacatg cgtatatcct tcttcccggt ccgcctaatg agttgaaacc aatgtttgtg
     7321 gaacaagtac gtcctttact agaggaacgt tttccttctg aagaaaaact aatttctaaa
     7381 gttttgcgct tttatgggat tggtgaatcg cgactggtca ctgagctaaa agatctgatt
     7441 gagacacaga tcaatccaac gatcgcacca tatgccaaac caaatgaagt gacattacga
     7501 ctgacagtga aaacaaatga tgttcaggca gggaatcaag ctcttttagc attagaagaa
     7561 aaaatccaag aacgagtagg ggaatatttt tatggatacg gcgatgataa ttcgctagca
     7621 aaagtcgtag tggaactact gaaagaaaac aagcagactg taacagcagc ggaaagtttg
     7681 acagccggcg ccttccaagc cgcattaggc gacattgctg gtgtatctga agtatttcca
     7741 ggcggattcg ttacctactc gctccaaaca aaagctggtt ttctggagat tgatccggag
     7801 ctattagcag aatatggaac agtcagcaaa gaatgtgtgg agcagatggc cattcaagca
     7861 cgtgagatag caagaacaga ttacagtgta gctttcaccg gagtggctgg accggacacg
     7921 ttagaaggtc aatctgcagg aacagtatgg atcgctattt catctaaaga cggcgtggtt
     7981 tcggaaaaat atcactttgc cagagatcgt gctgctatca gatataatgc ggttatgcaa
     8041 ggatttgatc ttttgagaaa acagctttta agaaaatgat aaatgcgaat atttgttcgg
     8101 ttttttcttg ctttttttca ctaaaaaaga tatgataagg ataatgaaat gttgctctga
     8161 tttgtaaata taaggaggat tatcgtattg gcagatgatc gtaaagcagc cttagatgct
     8221 gcactgaaaa aaattgaaaa gagctatggt aaaggctcta ttatgaaatt gggcgaaaaa
     8281 atcgaccaac aaatctctac aattccaagt ggctctcttg cgttagatgt cgctttaggt
     8341 gtcggcggtt acccccgtgg acgtatcatc gaagtatatg gacctgaaag ttcaggtaaa
     8401 acaacagttg cactacacgc tattgcagaa gtacaaaaaa atggcggaac ggccgctttc
     8461 attgatgctg agcatgcgtt agatccgcaa tatgcacaaa aattaggtgt gaatatcgat
     8521 gaactacttc tttcacagcc tgacacagga gaacaaggtc tagagatcgc tgatgcttta
     8581 gtatcaagtg gggctgtaga tatcgtagta gtcgattcag ttgctgcttt agttccacga
     8641 gcagaaatcg acggcgaaat gggtgactca catgtcgggt tacaagcacg tttgatgtct
     8701 caagcattgc gtaaactctc tggttcgatc aacaaaacaa aaacaatcgc tattttcatc
     8761 aaccaaatcc gtgaaaaagt aggcgtgatg tttgggaatc cagaaatcac tcctggagga
     8821 cgcgcattga aattctacgc aacgatccgt ttggaagtac gtcgtgcaga acaattgaaa
     8881 caaggtacag atattgttgg taaccgtaca aagatcaaag tcgtaaaaaa caaagtagca
     8941 cctcctttca aaatcgctga agtagatgtc atgtatggtt tagggatctc acaagaagga
     9001 gaacttctag acatggcagt agaaaaagac attgttgata aaagcggtgc ttggtattct
     9061 tataaagaag atcggattgg tcaaggacgg gaaaacgcta aaatctatat ggctaatcat
     9121 cctgaaatga tggcagaagt ttctgctcta gttagagcag cttatggtat tggtgaagaa
     9181 gtcgctgtcc cagaagatga aaaaggacaa gaagaattgc ctttagtaga agaataaaaa
     9241 ttatgataaa caaaaacggc tggtctgaag agtaggtact ctccttgacc tgccgttttt
     9301 ttattgaata aagaaatcgt gagttattta attatacgag tgggtacata aaataaaaat
     9361 atctgttcat ctacaaagaa taatttaata gaaaagtttc tcaatttaaa ggtttaccta
     9421 tatgaaacta gtgtcattta ttttaaaaac catcgtttaa tgaataagta gaaagccgtt
     9481 tctttgttta tttaaagttg acactacggg gtacaaacat tagaataaag ttgtgcatgt
     9541 tttttgtaca catatatgcg aagatgcata ttgacgagtt tagaataatt tatcatagaa
     9601 gctgtataga tgataaaaat gaaaaagata gtatggaggt ggattttatg gtattcaaca
     9661 ttctcctcgc tatcatcggt ttaattgtcg gtcttggttt agggtttatg gtcgcaaagt
     9721 cacgtcacga gaaagaaata gctggtgcac aatcatcagc tgctggaatc atcgatagtg
     9781 caaaaaaaga agcagaaact ctaaaaaaag aagcattgtt agaagctaag gaagaaaatc
     9841 agaaataccg ttctgaagtt gaaagtgagc tgagagaaag taagttagaa ttaaaatctc
     9901 aagagaatcg tttgattcaa cgcgaacaaa cgctgaatcg taaagatgac tcgttagaaa
     9961 aacgcgagaa ctcacttgaa agtaaagaag aagcacttag ttcaaaacag caattaattg
    10021 aagagcgaga aaaagacgtt gaaaaaatga ttgaagatca acaaaaagaa ctagaaaaaa
    10081 ttgctggttt atcaaaagag gacgcacgag atgtcatcat gaaatcgact gaagaagagc
    10141 tgaatcatga gttaactcta atggtaaaag aatctgaaca gcgtgccaaa gaagaagcag
    10201 atcgcaaagc gaaaaactta ttatcattag cgattcaacg ctgcgcagca gatcaagttt
    10261 cagaaacgac tgtttcagtc gttacccttc cgaatgatga aatgaaaggt cgtatcattg
    10321 gacgagaagg tcgtaatatt cgaacgcttg agacgctgac tggtatcgat ttaatcatcg
    10381 atgatacacc ggaagcagtt gttctttccg gattcgatcc aatccgaaga gaaatcgcac
    10441 ggatgacctt ggaaaaactg atacaagatg gacgaatcca tccagcgcgt atcgaagaaa
    10501 tggtcgagaa atctcgtaaa gaaatggatg aacgtattcg cgaatacggg gaagaggcag
    10561 cgtttgaagt cggtgctcat acacttcatc cagatctaat caagatcatc ggacgtctgc
    10621 gattcagaac tagttatgga caaaacgtct tgaagcattc ggtcgaggtt gcaaaattag
    10681 ctggtatctt agcagcagag cttggcgaag atatccaatt agcaaaacgt gccgggttgc
    10741 tccatgatat tgggaaagca cttgaccatg agattgaagg atctcacgtt gagatcggtg
    10801 cggaattagc tgccaagtac aaagaaaacc cagtagtaat caacgcgatc gcttctcatc
    10861 atggagatgt ggaagctact tctgttatct ctgtccttgt agctgcagca gatgcgctct
    10921 cagcagcaag acctggtgcg cgaagtgaat cattagaaaa ctacatccgt cgtttaagaa
    10981 gtctggaaaa tatttcgaac agcttcgccg gtgtagaatc aagttacgca gttcaagctg
    11041 gacgcgaagt tcgggtaatg gtcaagccag aagaaatctc ggatttagac gctgttcgtt
    11101 tagtacgtga tattcgtaaa cgaatcgaag aagaactgga ttatcctgga cacatcaaag
    11161 taatcgttat tcgggaaact cgtgcaactg attatgctaa ataataaaaa atacaaaaga
    11221 agagccgcgg caaaagccac agctcttctt tttttgtaag gcacgaaaaa acattgctat
    11281 cattattttt tattcaacat ctcaagatct ttctcgatcg tcgtattgat tcctttgatg
    11341 attgctggga tgaatcctgc ttcttctaag gcaactacac ctgcaacagt tgtaccgcca
    11401 ggagaagaaa cttggtctac aagttcccat ggatttttat cacttgaaag gaatttttga
    11461 caagaaccca taattgcttt tgcagcaatc tcagtggctg tctgtttgga caaaccattc
    11521 agtacaccag tccgtcccat cgcatcgatc agcatgtaaa cgaatgcggg tgagctgcca
    11581 gcaaggccaa tgaatgtact gaaatctgat tctgctaaag gatagacgct gccgattgct
    11641 tcaaaaatca tcgtagcttg ctgaagctgt tcctccgtgg cagcttcatt tttgcaaatc
    11701 gcagaaacgc ctgctcctac gctgacattg acattcggca tcacacgtac gatggaggcc
    11761 tgaggatctt caagcgctgt ttctaaatct attaatgatt ttcctgaagc aatagaaatg
    11821 aataagactt tctggctata ttctcttagt tcttccaata ctttttcaat gatttttggt
    11881 ttcacggcta aaagaacgat atctgattgt gatgcaactt cctgattact tttgcatgca
    11941 tggacgccag tttcagcagc aaatttttgt gcattttcaa aatgtccact ggaaacgaac
    12001 acctcttctt tcgagactgt tccttttttt aataaaccat caataatagc ttgtgccata
    12061 ttccctgtgc cgataaatcc tactttcatg ttatcctctc ctttagcatt aggatatctt
    12121 tctttttcta tcatcgcaag caaagaacag gtctaaagtg gaaaaattaa aataaataag
    12181 ataacattgg ggtatgacac aaaagtatac aagttaggaa tgggaaaatg tgaaaaataa
    12241 acgatacttg atgctgcaat ggataaagga acatagtctg attttaaaac tgattttttt
    12301 cggctccgtg ttagtctttg tggcaaatca agtcgcaaat attgctaatg gcatgagttg
    12361 gcaagatatt tggcaaagga tgggacaaca aagccgcatg acgatcctgc tcatgattct
    12421 ggctggactg ctgggtgtca ctccgatgtt gttgtatgat tgggtgacga tcagagtctt
    12481 agaaaaacaa ggaaaaccaa aaatggaaag aaaagagttt cttcttgctg cgtgggtaac
    12541 aaacacgatc aacaatctcg ccggttttgg tggtgtggta ggagcaagtc tgcgggctag
    12601 tttttatgga aagcagacaa atagaaaaat ggttttagcc actgtatcaa aagtcgcact
    12661 atttatgctg actggtttgt ctgtatggtc gtttcttaca tttattgatg tcttttttat
    12721 tcagtcagaa agtatttttc gaagctattg gatctggctt ttcggcggta gttttttagc
    12781 accagtcatt ttcttttttg cttatctaaa gagaaagaca ttgttcttcg agttttatcc
    12841 aaaaggcttg cttggtctgc tactggcatc ctttggacaa tggacgggag cccttgcggt
    12901 atttttgatc atcggcaagt tgatgagaat agatatttcg atgctttcga tctatcctat
    12961 gttcatgatt gctacattga taggaatgct gacgatggtc ccaggtggaa tggggacttt
    13021 tgacgttttg atgatcttag gaatgagtca attgtctgtt cggcaagatg tagcagtagt
    13081 ttggctatta tattatcggt tgttttatta tgttctacct tttatgacgg gacttctatt
    13141 gttcatcaca catacaggag tcaaaatcaa tcgattttta gataacattc cgcgactgtt
    13201 tttccaaaag gttgcgcata tcgtgctcgt aagtgctgtc tattttgcgg ggatcatgat
    13261 ggtacttcta tcaacaatca ctaatctgtc tgcaatcagt cggctatttc aagtattatt
    13321 gccgttctca ttcaattttt tagatcagac agtgaatcta ttggttggct ttttattact
    13381 cggccttgct agagcacttt cagtaaaagt caaaaaggca tttctgccta cgattgcttt
    13441 actatctttt ggtattttaa atacaatcac acgtacgtta tcttggcagt tgatattggt
    13501 atatgtcttg attctgttag ctgtgtggtt atccagaaaa gaattttatc gaacaaaatt
    13561 cgtttattct tggggagcta taatctttga tgctagcttg ttcggctttt tgtttatcgt
    13621 ttacgcaatt gcaggctatc acagcggacg ctttttagtc ggtcgaataa caggaaatca
    13681 ttttattttg tttccttcag atgatgtgtg gttctcaggg cttattggtt tgggcatctc
    13741 attgctagct ttagtggcgc tttatcaata tttagctgaa gatactacga agcttggcag
    13801 tgagtacgaa aaagaccgtt ttgaccgttt attggaaaca tacggaggca cagctacaag
    13861 ccatcagctt cgcttgtcag gttacagtta tttttattac caagaagacg accaagatca
    13921 agtcgttttt ggttatcaga tcaaaggaaa caaatgcttt gttttaggag atccagttgg
    13981 caatcctaaa atgatgcggg cagcaacgaa tcagtttatg aaacaagcag attgtttagg
    14041 ctatcagctt gcgttttata agatatcaga aagctacgta gtgatgcttc atgaattggg
    14101 ttttcacttc acgaaagtag gtgaagcagg gattgttgat ctagcaacag aagacttgtc
    14161 tcggctgagt cagcaacttg aatatcaacg tttgatgaaa gaaggatacc attttacttg
    14221 gtacgatcaa ctgccggaag aattacatca agctgtaaat gaagtttcac aaaaatggct
    14281 tcaaggtgca cgagaaaaat acttctctgt gggacgattt gataaatcat atgtattatc
    14341 tagcggtgtc ggtattgtcc gtaaagatca aaaagtcgtt ggcttcatca ccgctcagcc
    14401 gatgacgaag aagcaggcgg gatatgattt gttgcgggtc ttacctaaag aatctgagga
    14461 attagctgac tatctgttag ccaatctgtt tattgtatac gagaaaatgg gctactcgga
    14521 agccaatcta ggcttagctc cattagctaa tgttggagaa acagattttt ctttctttca
    14581 agaaaaagtc atgcatatcg tatataatta tggtaatttt ttctattcct tccagtctgc
    14641 ttatgaaaag aaattaaaat atgtcgatca ttgggaagga cgttattttg cttataggaa
    14701 aggaagtaat ttcggatttg ctaccatgca gctactcagt ctgataggaa aagaaaaagg
    14761 aaatgttccg agtttggcag aagaagtgct cacagaatta taagaatcac tagaacttgt
    14821 ttggattatg aagagaaaat tctgtgaaag ctgttgaaac gatgacaaaa tcaaccacta
    14881 ggcagacata agctatactt ttatttttcc ctatgataga atacttgcga cttagacttt
    14941 tcgaatgggt gagaggatgg aattgattga aaaagcgcca gccaagataa atttaggact
    15001 ggatgtatta tatagacggc aagatgggta tcatgagttg gaaatggtga tgtcgagcgt
    15061 agacttggca gatcatctat tttttgaaga aataacagaa gacaaaatca ttgtagaaac
    15121 aaataaagca tttttaccgg tagatgaacg gaacaatgtt tatcaagctg ctttattgct
    15181 aaaaaataaa tacaatatca aaaccggtgt caagatcttt gttaagaaga atattccagt
    15241 agcagctgga ttaggcggag gaagtacaga ctgtgcagct gctctaagag ggatcaacca
    15301 attatggaat ctgggctgtt cgttagaaga actggcagaa atcggtttgg aagtcggtac
    15361 agatgttcct tactgtgtct acggaagtac ggcatttgtc ggtggcaaag gggaagaaat
    15421 ccaagtatta ccttctatgc cacagtgctg ggttgtgttg gtcaaaccaa gaatgagtgt
    15481 ctctacacgt acagtgttta gagaagtaga tacgcaagcc atccaacatc aagacatcca
    15541 cggacttgct gaagccatcc gagagcaaga ttatcagcag atgatcaaaa aaatgggaaa
    15601 cagtttagaa gatataacgg gaaagcggca tccagtcatc catcaaatca aagagcgtat
    15661 gatcaagtac ggagcagatg ctgcattgat gagtggaagc ggaccaaccg tttttgcctt
    15721 gtgtcaacat tattcaagag cgcagagagt atataatggg ttaaaaggtt tttgtgagga
    15781 agtttattta gtacgaaccc tgcaataaaa tataagaagt aaaataggtg atgaaaaact
    15841 gaggaatcct aaaatggtat tcttcagttt tttattgtag tgaaatggct agatatgagc
    15901 agcaactcat ccattttacg atttaatcaa gaatatacat gcttaaatca gaaattgatg
    15961 gatttctatt gactgtgttt ttttattttg atacattagt tcacgttaat aaatcgtaaa
    16021 attccgattt gttcattgaa aggagcatat aaatgaaaaa atatcttttg acagctacgg
    16081 taatgatagg tgctttactg tttgcagcat gtggaaatac gaacaaggaa gcagacaaaa
    16141 aagaggattt gacgatcgtt acgacttttt atccgatgta tgattttaca aaagagatcg
    16201 taggggatga aggaaacgtg aaacttttga tccctgctgg aacggagccg catgatttcg
    16261 aaccaagtgc aaaagaaaga gcagaaattt ccgatgcaga tgttttcgtg tataatagtt
    16321 cagacatgga attttttgtg gattcgttaa aggattcagt ggatagcaaa cagaccttga
    16381 tgatcgaagc agctaaagga atcgaccgtt tagaatcgca agaagcagac gaacatgaag
    16441 aatcagaaga agggcatggt cattcacatg aatacgatcc acatgtttgg ctcgatccgg
    16501 tattagctat aaaagaagtc agaacgattg ctgaagaatt aggcgaaaaa tatccagata
    16561 aaaaggaaac ttttacgaag aatgccgatg cttatatcaa aaagcttgaa gcactggatc
    16621 aaaaatactc cgatgagcta aaagatgcaa caaaccgcac atttgtcact cagcatgctg
    16681 cttttgctta tttagcgaat caatacgact taaaacaagt agcgatttcg ggcgtttcac
    16741 cagatcagga acccacacca agtcgcttag ctgagctaaa agaatttgtg aagaaaaaca
    16801 acatcaaagt gatttatttt gaagaaaatg cctcttcgaa ggtagctgag acattgtcga
    16861 atgaaacagg agtgaagcta gaagtgttga atcctttaga aagcttgact aatgaacaaa
    16921 tcaaagcagg cgaaaattat atttctgtca tggaaaaaaa tctgaaagct ttaaaagaaa
    16981 gtatcaatta agttttagaa tggaggttgt aaggtgcgtt atatcgaaat atctgatttg
    17041 accttttact atgatgatga acctgtacta gaagatgtct cttatcatgt agatgcgggt
    17101 gaatttgtca ttttaactgg agaaaatgga gcagccaagt caaccttggt gaaagcatcg
    17161 ttaggattac tgaaaccttc aaaaggaatg ataaagattg ctgagaaaaa tcaagaagga
    17221 aaaaaattaa gtatcggtta tattcctcaa caagttgcat ctttcaatgc aggttttcct
    17281 agtacggtga tcgaattagt ccgttcagga agatatccaa gaggccgctg gttcaagcgt
    17341 ctctctgaaa aagatcatca acatgtacag cgtgctttag aagctgtagg gatgtgggat
    17401 atgcgaaatc ggcgaatagg tgaattgtct ggcggacaaa agcagcgaat cagtttagcg
    17461 cgtatttttg ctactgaccc tgacttattt gttcttgatg aaccaacaac aggtatggat
    17521 gaagaatcca gaaatgaatt ttatcgtctt ctccgacata gtgcgcatgt ccatgggaaa
    17581 tcagttctaa tgatcaccca tgatcatgaa gatatcaaac agtatgcgga cagacagatc
    17641 cgactggtga gaaaggagga ttctcaatgg cgttgctttc atatgaattc atgagaagag
    17701 cgtttcttgc cgctatcttc attgccggaa tcgccccgat gctaggtgtg tttttagtga
    17761 tcagaagaca atcgctgatg gctgatacgc tttctcatgt ctctttagca ggggtagcgt
    17821 taggattctt tttgaacctt aatcctacac ttacgaccat gctagtcgtt gtattagcag
    17881 ctgtgatatt ggagtatctg cgaacgatct accgaaccta ttcagaaatc tccattgcca
    17941 ttttaatggc tggtggtttg gcgctagcgt tagtattgat gaatttgagc ggagggaatt
    18001 cagcaacaag tatccaatcc tatctgtttg gttcgatcgt gaccattaca tgggagcaag
    18061 tcatcttttt aggaatctta ttcgttgcca tcggtatatt gttcttttta ttcaaacggc
    18121 cgatgtatgt tctgactttt gatgaagata ccgcccacgt ggatgggcta cccgttcatt
    18181 tgatgtcgat gatgttcaat gtgattactg gtgtggctat tgctgtgatg atcccgattg
    18241 ccggtgcact tttgatatcc gcaatcatgg ttcttcccgc agcaatcggt atgcgattag
    18301 gcaaaagctt caatgtcgtg attgctatca gtgtttgtgt aggatttcta gggatgctgt
    18361 caggactaac tagctcgttt tatctagata cacctccagg agcaacaatc acattagtct
    18421 ttattgcttt gttcctgatt gtaaatatca tcaaacgaat attggttgcc ttcagaagaa
    18481 aaaataacta aattataaag aaaagaagcc ttataaaaac gaaaaatttt ttataggctt
    18541 ctttttattt taataaatac cgaactattt tcttattttt tcataaaatg ctcgaatttt
    18601 tgaagaaaaa cttatataat gtaaaacgat aaaatttctt aaaaggagtt ggaataggtg
    18661 aaagtacgtc gcagtgaacg tctagttgac atgacacagt atttattgaa tcacccgcat
    18721 gaattgattt ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc ttctatcagt
    18781 gaagatttgg gaatcatcaa gaaaacattt aaagaacgag ggtatggaac cttggaaaca
    18841 atcccgggtg ctgcaggtgg tgtattgttc atccctgaaa tttcatatga agaggcgaaa
    18901 aaatatattg aatctctagc agaacgtcta tcagaacaag accgtctgct tccaggagga
    18961 tatgtttatc tctctgattt gttaggagaa cctgaactgc tgcgtcaagt tggaagaatc
    19021 attgcttcta aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc cacaaaaggc
    19081 gtacctattg cccaagctgt gtcctattat ctgaatgcgc cattcgttat tgtacgccgg
    19141 gactcaaaaa taacagaagg atcaacagta agtgtcaact atgtgtctgg atcttctgaa
    19201 agaattgaaa agatggaact gtctaagaga agtttgaaac gtggttcacg tgtccttgta
    19261 gtggatgatt tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt gattgaagaa
    19321 ttcgaatcag aacttgttgg catcactgta tttgccgaat caaaattcaa cggtcatcgc
    19381 gccatcgatg attatacgtc actgctttat gtcaaagatg tagatactca aacgaaaaat
    19441 attacggttg tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa gccgcttagt
    19501 ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac attttttcat aaatttggct
    19561 taataccgag aaactggctt ttgaacaccg tttatctaga ggttgtgaaa tcagcgtcct
    19621 gtcctgagta ttagctcaat aaacggaaaa attgctcctc atattttgcc gtttattgag
    19681 ctaataccga aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa gcctaccgtt
    19741 taactctaag tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta tgagtagtgt
    19801 gacttgtgac cgaggagttg gcaggagagc accggttacc aaatagatag gcccgttatg
    19861 gtctatctat ttttttatcc agaaaacgct gggataaaag aaaatcactt gttttgttga
    19921 aaaaatgggt atactgggat aaactgaaat cagcaaacta tatttaggag tgacgcagtt
    19981 ggacgcacgt tatgcaatta ttttggcggc agggaaaggt acccgcatga agtcaaaatt
    20041 atataaagtt ttacatcccg tttctggtaa acctatggta gaacatatca ttaatcgggt
    20101 cagtgaaacg aaaccagatg aagtgattac gatcgttgga cacggagcag aacaagttaa
    20161 agcacaacta ggagaacgca gtaaatatgc gttacaagca gaacaattgg gaacaggaca
    20221 tgctgttttg caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt tagttatcag
    20281 tggcgataca ccattattga ctactgaaac gctgaacaat ctttttgaat atcaccaagg
    20341 aaaaaatgca agtgcaacga tcttgacggc acaagcagaa aacccaacag gatatggacg
    20401 aatcatccgt gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag atgcaacacc
    20461 agaagaagct cttgtacaag aaatcaatac aggaacttac tgttttgaca acgaagcttt
    20521 gttcgatgcg ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact atctaacaga
    20581 tatcatcgaa atcttaaaag aagaaggcca tacagttgct gcttatcaaa cggatgattt
    20641 tgaagaatca atgggagtca atgatcggat cgcgttagct aaagcaaatg agatcatgag
    20701 aaagcgaatt aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt cagcaacgac
    20761 atacatcgat gcaggcgtag aaatcggtcc agacacattg atcgaagcag gtgtacaaat
    20821 ccaaggaaat acagtcattg gttctgattg tgtcatcggg tcacattcaa aaatcgtaga
    20881 cagtaggatt gaggatcacg tcgtcattga aaactcagtc attgaaagca gccatgtcaa
    20941 gaaacatgca gatgtgggac cttatgcaca tttacgacca aaagctgaaa ttggcgaaaa
    21001 cgtccatatc ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag gaactaaagt
    21061 cggtcacttg acttatgttg gtgatgctac gctgggagaa gaaatcaatg tcggatgtgg
    21121 tgttgtcttc gtcaactatg atggaaagaa caaacatcat acgactgtcg gcgaccacag
    21181 ctttattggt tctagcacga atatcatcgg acctgtcgag gtagctaaaa acagttcgat
    21241 tgctgccggg tcaacaatta cagacaatat tccagaatat gcgctggcaa ttgcgagagc
    21301 aagacaagtc aataaagaag gatatgcaaa aaaactccct tatctaaact aagacttttg
    21361 caaaatcact tgatttattt aaaaaatatg actactattt attaggtaga attctagagt
    21421 tgtgaaaaag aaagtggagg tccccatgtc aaaacattac tttgacccaa gattaaaaat
    21481 ttttgcgtta aattccaatc gcccattagc agagaaaatt gcagcagcag tcggcgtcga
    21541 gttaggaaaa tcatctgtta ctcaatttag tgatggagaa attcaagtaa acattgaaga
    21601 aagtattcgt ggatcgcatg tctatgttat tcaatcaacg agcagcccag tcaatgataa
    21661 cttgatggag ttgctgatca tgattgacgc actgaaacga gcaagtgcaa aaacaatcaa
    21721 cgtagtcatg ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg cacgggaacc
    21781 aatcacagcg aaattagtag caaacatgat tcaaaaagct ggtgcaacaa gaatgctgac
    21841 tttggacctt catgcagtcc aaatccaagg tttctttgat attccagtcg atcatttgat
    21901 gggtgcgcca cttattgcca attatttctt agaaaaagga atcaaaggcg atgatgttgt
    21961 cgttgtttca ccagaccatg gtggtgtcac acgggcacgt aaattagcgg aatttttgaa
    22021 atctccgatt gcgatcatag acaaacgccg tccaaaagca aatgttgcag aagtaatgaa
    22081 tatcattgga caagtcaaag gcaaaacttg tgtactgatc gatgatatga tcgatacagc
    22141 aggtacgatc acattggctg ccaatgcttt gaaagaagct ggtgcaacta gtgtgtatgc
    22201 ttcatgtaca catccagtat tatccggccc tgctttacaa agaatagaag attcagctat
    22261 cgagcgtcta gttgttacag actcgatcta tcttccagaa gatcgtaaaa tcgacaaaat
    22321 cgatgaagta agtgttggcg gactaatggg agatgcaatc aaacgtatcc atgaaaacaa
    22381 accagttagt ccattgtttg aaacaaaaaa taaataataa ataaactcac aaaggggagt
    22441 ccaattgggc tgagattgaa tctatttcta aacccttcgt acctgtatcg gttatgcgag
    22501 cgtaggaatt gtgaataaac agctgtttga ctattttcga aagctgcttc ctcctttgtg
    22561 acttttttat tcaaaggagg aagttttttt atgggaaaaa atagagtttg ggtagaagga
    22621 acgattgttg cagcgttagc gatggtgttg tcgttgattc cgatccagat cggcagcagt
    22681 ttttctattt cattagggca gattccattg actctttttg cgttaagaag agggacaaag
    22741 gcagggatgc tggcaggttt catttggggc atcctacatt ttccgttggg acaagtttat
    22801 tatttaagtg tcgttcaagt gttgattgag tacccgattg cctatacttt tgcaggcgca
    22861 gcagggctca tggcttcaaa tgtccaaaaa ggctatgtag acgataatac gaaacaaatt
    22921 agaaaaagca tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt ttggcatttt
    22981 attgcaggag tggttttctg gggagcgtat gcactatggg ggatgaatcc ttggctcttt
    23041 tctttcgtaa tgaatggtgc cagcgggttg agtacagcta ttgtaacgat catcgtttta
    23101 ttagggatcc aacaaacgac tccttcgatt tttgtgccag ataaacgaca ggagctgtaa
    23161 ataaaaaaga agcaagaaac ttgagacaga atcatgtcct agtttcttgt ttccttttta
    23221 ttttttgata agaaatagat ctacttcaat ggcagataag aaagatcggc atcttctacc
    23281 aattgccagt gtcgatcggt gacagagtag attaaagtag cgatgatttc ctcttctcga
    23341 taccgggtgc cttttaattt ccaaatataa tcttcaatca catagattgg gatacgatat
    23401 ttaccatcga tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg catcaaatta
    23461 cgcatttctt tcaataattc cacactgatt ttttcaacag gtgtttcttt tttgaaaaaa
    23521 gattttttta cattgatctt atctgacaag atcgaactta atttactagc aaaaatccta
    23581 taaaattcat ctaaatagcg ataataaatc cgattcagct gatcattaag atcgaagtat
    23641 acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca
    23701 aaatataata attctgaaat ttctaaaggt gtcaattctt tcagcatcgt taaatcagta
    23761 aagtcgatcc acttcaattc atcgaatcca cgcctgttac gagaagagga aaagaaacgc
    23821 tgaatatttt ctgtaccacg gattactttc agccctgtat gggattcata ttctccagca
    23881 gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat gcacgatact gtttgcaaag
    23941 tcagtagcag tcagcccttt tgttaaaaca gcattacttg tggtatcaat atgtacataa
    24001 actgactcag tcatcatgtt cctcctctta tttcctctta atctctttta ttttacaaca
    24061 tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc attacaaaat cacgttttca
    24121 ttgaaaacac tctcattttc aattaatttt tgtgattttt gtattttttt atttaaagga
    24181 ggttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    24241 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    24301 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    24361 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    24421 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    24481 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    24541 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    24601 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    24661 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcgcacgg
    24721 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    24781 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    24841 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    24901 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    24961 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    25021 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    25081 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    25141 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    25201 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    25261 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    25321 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    25381 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    25441 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    25501 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    25561 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    25621 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    25681 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    25741 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    25801 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    25861 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    25921 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    25981 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    26041 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    26101 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    26161 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    26221 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    26281 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    26341 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    26401 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    26461 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    26521 ggaacgcttc gtgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    26581 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    26641 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    26701 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    26761 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    26821 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    26881 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    26941 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    27001 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    27061 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    27121 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    27181 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    27241 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    27301 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    27361 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataaacagaa
    27421 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    27481 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    27541 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    27601 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    27661 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    27721 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    27781 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    27841 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    27901 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    27961 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    28021 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    28081 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    28141 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    28201 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    28261 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaaa acgaatgaaa
    28321 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    28381 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    28441 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    28501 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    28561 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    28621 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    28681 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    28741 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    28801 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    28861 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    28921 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    28981 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    29041 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    29101 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    29161 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    29221 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    29281 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    29341 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    29401 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    29461 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    29521 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    29581 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    29641 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    29701 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    29761 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    29821 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    29881 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    29941 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    30001 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    30061 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    30121 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    30181 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    30241 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    30301 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    30361 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    30421 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    30481 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    30541 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    30601 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    30661 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    30721 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    30781 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    30841 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    30901 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    30961 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    31021 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    31081 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    31141 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    31201 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    31261 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    31321 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    31381 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    31441 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    31501 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    31561 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    31621 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    31681 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    31741 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    31801 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    31861 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    31921 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    31981 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    32041 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    32101 aaaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    32161 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    32221 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    32281 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    32341 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    32401 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    32461 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    32521 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    32581 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    32641 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    32701 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    32761 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    32821 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    32881 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    32941 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    33001 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    33061 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    33121 aaagtggatg actgggtcaa gatgcttctt tatctttctg tataccaatt agaattttta
    33181 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    33241 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    33301 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    33361 ctatggatga cgaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    33421 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    33481 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    33541 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    33601 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    33661 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    33721 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    33781 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    33841 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    33901 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    33961 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    34021 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    34081 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    34141 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    34201 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    34261 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    34321 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    34381 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    34441 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    34501 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    34561 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    34621 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    34681 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    34741 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    34801 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    34861 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    34921 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    34981 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    35041 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    35101 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    35161 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    35221 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    35281 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    35341 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    35401 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    35461 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    35521 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    35581 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    35641 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    35701 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    35761 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    35821 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    35881 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    35941 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    36001 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    36061 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    36121 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    36181 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    36241 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    36301 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    36361 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    36421 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    36481 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    36541 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    36601 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    36661 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    36721 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    36781 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    36841 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    36901 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    36961 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    37021 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    37081 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    37141 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    37201 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    37261 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    37321 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    37381 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    37441 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    37501 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    37561 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    37621 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    37681 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    37741 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    37801 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    37861 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    37921 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    37981 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    38041 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    38101 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    38161 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    38221 cgcagattac atccacgtag atgtaatgga tggccgattt gtcccaaata tcacattagg
    38281 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    38341 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    38401 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    38461 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    38521 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    38581 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    38641 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    38701 tgcaggggcg gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg
    38761 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    38821 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    38881 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    38941 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    39001 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    39061 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    39121 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    39181 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    39241 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    39301 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    39361 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    39421 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    39481 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    39541 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    39601 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    39661 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    39721 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    39781 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    39841 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    39901 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    39961 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    40021 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    40081 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    40141 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    40201 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    40261 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    40321 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    40381 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    40441 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    40501 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    40561 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    40621 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    40681 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    40741 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    40801 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    40861 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    40921 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    40981 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    41041 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    41101 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    41161 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    41221 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    41281 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    41341 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    41401 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    41461 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    41521 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    41581 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    41641 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    41701 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    41761 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    41821 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    41881 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    41941 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    42001 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    42061 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    42121 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    42181 aacagcgcct gaatcttcag caacagaaga atcaacaaca gtgcctgaat cttcagcaac
    42241 agaagaatca acaacagtgc ctgaatcttc aacaacagaa gaatcaacaa cacctgcgcc
    42301 tacaacacca tcaacagatc aaagtgttga tacaggaaac ggcacaggaa gtagtactcc
    42361 ggctccaacg ccaacaccaa cacctgaaca accaaaacct gtaacacctg ctccagctcc
    42421 ttctggaagt gtgaatggtg cagcaatcgt agcagaagct tacaaatata ttggtactcc
    42481 atatgtttgg ggcggtaaag atccaagtgg ctttgactgc tcagggttca cacgctatgt
    42541 ttacatgcaa gtaactggtc gtgacatcgg tggttggaca gttcctcaag aatcagctgg
    42601 aacaaaaatt tcagtttctc aagcaaaagc tggagacttg ttattctggg ggtcacaagg
    42661 cggaacttac cacgtagcga ttgctttagg cggaggacaa tatatccatg ctcctcaacc
    42721 aggcgaaagc gtaaaagttg gttcagttca atggtttgcc cctgactttg ctgtcagcat
    42781 gtaagaatga ttaatcaaaa aga
//
LOCUS       DACCVU010000011        40801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCVU010000011 DACCVU010000000
VERSION     DACCVU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.011"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1312)
                     /locus_tag="IVW29_000512"
     CDS             complement(<1..1312)
                     /locus_tag="IVW29_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7838018.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLY"
     gene            complement(1314..1982)
                     /locus_tag="IVW29_000513"
     CDS             complement(1314..1982)
                     /locus_tag="IVW29_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7838019.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2112..2696)
                     /locus_tag="IVW29_000514"
     CDS             complement(2112..2696)
                     /locus_tag="IVW29_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838020.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3027..3260)
                     /locus_tag="IVW29_000515"
     CDS             complement(3027..3260)
                     /locus_tag="IVW29_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7838021.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3275..4225)
                     /locus_tag="IVW29_000516"
     CDS             complement(3275..4225)
                     /locus_tag="IVW29_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7838022.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4222..5064)
                     /locus_tag="IVW29_000517"
     CDS             complement(4222..5064)
                     /locus_tag="IVW29_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7838023.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5066..5785)
                     /locus_tag="IVW29_000518"
     CDS             complement(5066..5785)
                     /locus_tag="IVW29_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7838024.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6477..6635
                     /locus_tag="IVW29_000519"
     CDS             6477..6635
                     /locus_tag="IVW29_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838025.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6707..7933
                     /locus_tag="IVW29_000520"
     CDS             6707..7933
                     /locus_tag="IVW29_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7838026.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8021..8413)
                     /gene="rpsI"
                     /locus_tag="IVW29_000521"
     CDS             complement(8021..8413)
                     /gene="rpsI"
                     /locus_tag="IVW29_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7838027.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8429..8872)
                     /gene="rplM"
                     /locus_tag="IVW29_000522"
     CDS             complement(8429..8872)
                     /gene="rplM"
                     /locus_tag="IVW29_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7838028.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9391..9858)
                     /locus_tag="IVW29_000523"
     CDS             complement(9391..9858)
                     /locus_tag="IVW29_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7838029.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9978..11516)
                     /locus_tag="IVW29_000524"
     CDS             complement(9978..11516)
                     /locus_tag="IVW29_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7838030.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11596..12282
                     /locus_tag="IVW29_000525"
     CDS             11596..12282
                     /locus_tag="IVW29_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7838031.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12483..13010)
                     /gene="msrA"
                     /locus_tag="IVW29_000526"
     CDS             complement(12483..13010)
                     /gene="msrA"
                     /locus_tag="IVW29_000526"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7838032.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13094..13771)
                     /gene="pgmB"
                     /locus_tag="IVW29_000527"
     CDS             complement(13094..13771)
                     /gene="pgmB"
                     /locus_tag="IVW29_000527"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7838033.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13768..14897)
                     /locus_tag="IVW29_000528"
                     /pseudo
     CDS             complement(13768..14897)
                     /locus_tag="IVW29_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(14897..17080)
                     /locus_tag="IVW29_000529"
     CDS             complement(14897..17080)
                     /locus_tag="IVW29_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7838034.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17077..18102)
                     /locus_tag="IVW29_000530"
     CDS             complement(17077..18102)
                     /locus_tag="IVW29_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7838035.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18147..18974)
                     /locus_tag="IVW29_000531"
     CDS             complement(18147..18974)
                     /locus_tag="IVW29_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7838036.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18971..20038)
                     /locus_tag="IVW29_000532"
     CDS             complement(18971..20038)
                     /locus_tag="IVW29_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7838037.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20064..20900)
                     /locus_tag="IVW29_000533"
     CDS             complement(20064..20900)
                     /locus_tag="IVW29_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838038.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20897..21766)
                     /locus_tag="IVW29_000534"
     CDS             complement(20897..21766)
                     /locus_tag="IVW29_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838039.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21836..23119)
                     /locus_tag="IVW29_000535"
     CDS             complement(21836..23119)
                     /locus_tag="IVW29_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7838040.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23133..24818)
                     /locus_tag="IVW29_000536"
     CDS             complement(23133..24818)
                     /locus_tag="IVW29_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7838041.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25093..26118
                     /locus_tag="IVW29_000537"
     CDS             25093..26118
                     /locus_tag="IVW29_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838042.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26713..30366)
                     /gene="rpoC"
                     /locus_tag="IVW29_000538"
     CDS             complement(26713..30366)
                     /gene="rpoC"
                     /locus_tag="IVW29_000538"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7838043.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30445..34062)
                     /gene="rpoB"
                     /locus_tag="IVW29_000539"
     CDS             complement(30445..34062)
                     /gene="rpoB"
                     /locus_tag="IVW29_000539"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7838044.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34790..35758
                     /locus_tag="IVW29_000540"
     CDS             34790..35758
                     /locus_tag="IVW29_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7838045.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35776..36681
                     /locus_tag="IVW29_000541"
     CDS             35776..36681
                     /locus_tag="IVW29_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838046.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36678..37484
                     /locus_tag="IVW29_000542"
     CDS             36678..37484
                     /locus_tag="IVW29_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7838047.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37641..38609
                     /locus_tag="IVW29_000543"
     CDS             37641..38609
                     /locus_tag="IVW29_000543"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7838048.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38926..39450)
                     /locus_tag="IVW29_000544"
     CDS             complement(38926..39450)
                     /locus_tag="IVW29_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7838049.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39737..>40801
                     /gene="scm"
                     /locus_tag="IVW29_000545"
     CDS             39737..>40801
                     /gene="scm"
                     /locus_tag="IVW29_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7838050.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12004 a         8431 c         7245 g        13121 t
ORIGIN      
        1 tataaagaaa ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt
       61 taataaggtc agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa
      121 atagaaataa attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc
      181 caccggataa atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa
      241 ttttttcttg ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg
      301 attctgtgaa aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt
      361 ttttattttc gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta
      421 aggttgtttt ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat
      481 caaaatttat gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg
      541 cacttacctt ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta
      601 atgcgttatt gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt
      661 taacaaatag aacttgatca attaattctt tcaaatagta aaaacgatgt tcagctataa
      721 caatagtaaa tcctttattt tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt
      781 tgaagtccaa atttgaagac ggttcatcca atataattaa tttagagttt agcgataaac
      841 tacatccaat agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta
      901 atatatttga aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt
      961 ttgtaaatcc aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga
     1021 attgtcctct aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta
     1081 aggattgtag cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc
     1141 ttggaataag tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag
     1201 taatcaatgt aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct
     1261 taacatcctt atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc
     1321 aaaatactta agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg
     1381 tttgttttat agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta
     1441 gcggtcattt cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc
     1501 aaatattgca gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta
     1561 atcattctta tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac
     1621 attatttttt tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag
     1681 acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag
     1741 gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact
     1801 aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc
     1861 aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa
     1921 aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac
     1981 actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg
     2041 ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa
     2101 gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
     2161 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
     2221 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
     2281 atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat
     2341 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg
     2401 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct
     2461 ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca
     2521 ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc
     2581 ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc
     2641 cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac
     2701 ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa
     2761 ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact
     2821 cattccttag agctaaacta atgagaaaga gcagaacaac tgatacaaaa agcaatccta
     2881 caagaattaa tagactcatc taaatcattc accttttcta aaataatccg agaaagattt
     2941 aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt
     3001 cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat
     3061 tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta
     3121 gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt
     3181 tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta
     3241 gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt
     3301 tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa
     3361 ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca
     3421 ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt
     3481 tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa
     3541 tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa
     3601 caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag
     3661 aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat
     3721 ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta
     3781 accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta
     3841 tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt
     3901 ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg
     3961 cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa
     4021 taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta
     4081 aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt
     4141 tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca
     4201 gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt
     4261 tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc
     4321 tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac
     4381 tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc
     4441 tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc
     4501 tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc
     4561 ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat
     4621 aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa
     4681 aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat
     4741 aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac
     4801 actattgctt tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc
     4861 aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga
     4921 aatagcatca cccataagag acattcctct taaaataata aaacaaccta ctgttccaga
     4981 aacaatccct ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag
     5041 accattaata aaattttgaa gcatattatt ccccctctcc aataaatata gtatctccat
     5101 aggctttttt taaattttct tcattaaata cagattctac acttccgtga gcaattagat
     5161 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
     5221 caattagcac tgtttttccc tgatgtttca atttttttaa cgtatccata attattcgtt
     5281 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
     5341 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
     5401 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
     5461 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
     5521 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
     5581 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
     5641 ttaacattgc tttaagcaat gtggattttc cagcaccatt tggaccaata atacctgtta
     5701 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
     5761 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
     5821 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttatagtt tagatttttt
     5881 tctgttgctg aagattagaa tgacaaaact atttgaaact acgcgtatag taagtagctt
     5941 tcaaaatata taaaaaagga gcgttctcaa atgagaacat gctcctaaaa ctactttatt
     6001 ggcgtatgga aaaattatac ttgaagtgga tagcgggttg aataagttga taagcatcaa
     6061 aacatttata aaaattattc aagactatgt ctgttccata tacaaatatg aaaatcagta
     6121 aactccattt aacttttttc gtagtgtgtc tttttataca tgtttatcgt tattaatagt
     6181 gaataggaaa aaaactattt ttgatcattg acaattaaat acaatttttg aataacgatc
     6241 cctgctattc gagctagaaa ctcaagtacg ccatgacctg attagcgaca aatacttaga
     6301 gatttctttt tgttctggga aaacaaaatg ccatgacaat agcagcaaat aatgatactt
     6361 ccgtaaggct cgccgtaaac tggggagagg tgaaattcct atgtgcttac ccaaagcaat
     6421 tcaaaaattt cataactact attttaggag atgattttat agaatccaga gtctacctga
     6481 ataaaaaaca aacctgtgca tttctaggaa tcgataataa tactctagat aagtggattc
     6541 gtatatataa tttcccagta ataagagttg gtggtgtagt tcgctttgac gtagaagata
     6601 ttaaagactt tatgaataca tttaaaataa cttgaaattt tcttctcgcc gcatatacta
     6661 aaattagtga aatatactgc cttacttttt ttaaggagtg aggattttgc ctacaaagct
     6721 ttcaaatggg aaatataaaa ctaatctgag atatccaaaa aggtttaaag aaataacagg
     6781 aattgcatca gaaaagtatc aaaaaacatt tccaaatagg caattggcga ttaaagccga
     6841 aaatgatatg aagaagaaaa tagaaaaagt tttgagagag gagaatgcaa actcattaga
     6901 attaaaagga aaaataacgt ttaaaaaatt ttacgaatct aaatggcttc ctagatatga
     6961 attaggtcaa acaattagat caaacaggcc tcctagtgat ataaccatta gtaatacaaa
     7021 agatatattt cggttgcata ttttacctat gtttggtgaa tacgcaatga attatctaaa
     7081 tatcaatact gagataatat cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat
     7141 aaaaatcata aaaggatatg ttcgttccat gttcgatatt gcagaaatac taaattatat
     7201 cgaatttaat cgaactacta aaattattca aagtatcact gcacctaaaa aaaatgcttt
     7261 agaagaaaaa agaatacaag aaggtaagca agcactatca tcaaaagaac ttactaattg
     7321 gatagaagca gtaaatgatg attttaataa tcatttactt acttttcatg attacacact
     7381 ttttatgatt accctgtatc ttggtgatag aaaaagtgag acttacgctt tgcaatggaa
     7441 atatattgat tttgaaaaac aaacggtccg tcttaaacat actttagata aatatcaaag
     7501 aaaaaagttt acaaaaggtc gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac
     7561 attgctaagt gaatggaaat cggtacaagc tgatcaattg ttgaaattaa aaattaagca
     7621 aacgttagat cagtatctct ttacttatac taaaccatct ggagaagtaa attgtcctgt
     7681 tcatgccgat tacctaaact atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt
     7741 tcatttatct ccacacaaac tccgtcatac ttatgcaact atagctagac aaggtggagc
     7801 agatatgaat caaatttcaa atgctttaac acactcagat atttctacaa caaagatata
     7861 tgtgaacaca ccagatatag ttgataaggc tgtttttgaa gcttttcaga gaggattgaa
     7921 taagtgtgat tgatatgtgt tcttaaatgt gttcttaaaa acaaaagaag cactttccaa
     7981 ataattgaaa agtgccagaa acgttgatat ataaaggata ttaacgtttt gagaattgtg
     8041 aagctttacg tgctttctta agacctggtt ttttacgttc gaccatacgt gcgtcacgag
     8101 taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct agcaatgcac
     8161 gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg tttacgttaa
     8221 caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg attacttcac
     8281 gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta attttgccag
     8341 ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg ccgatatatt
     8401 gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct aaaacttctg
     8461 gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt gtaaattgtt
     8521 tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc aaacgacgag
     8581 aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg tagttgctgt
     8641 gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg tctgcattga
     8701 tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta tttttaccac
     8761 gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt gcgtctacta
     8821 cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc acgttatttt
     8881 cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct ctttgtgggg
     8941 caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt ttttctgctt
     9001 gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa agggtcgttt
     9061 cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa ccccttattt
     9121 tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat
     9181 aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc
     9241 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
     9301 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac
     9361 ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa
     9421 atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc
     9481 ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc
     9541 tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg
     9601 gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga
     9661 accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat
     9721 gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt
     9781 ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta atacttcttt
     9841 ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt
     9901 agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt
     9961 tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat
    10021 tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga
    10081 tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt
    10141 gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat
    10201 gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc
    10261 tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt
    10321 tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc
    10381 ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct
    10441 gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca
    10501 cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct
    10561 ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca
    10621 ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc
    10681 tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct
    10741 acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg
    10801 tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt
    10861 gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga
    10921 atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca
    10981 gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag
    11041 tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc
    11101 cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat
    11161 tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc
    11221 caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc
    11281 gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct
    11341 tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt
    11401 gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg
    11461 aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt
    11521 tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga
    11581 aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta
    11641 tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt
    11701 gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc
    11761 caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa
    11821 gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg
    11881 acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg
    11941 gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc
    12001 ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc
    12061 tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc
    12121 ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt
    12181 ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt
    12241 ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac
    12301 gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta
    12361 ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag
    12421 ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct
    12481 tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga
    12541 taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga
    12601 taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct
    12661 tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc
    12721 actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt
    12781 tgctgccaat agatttccac caactgatca taagaaataa cttctggatc atattcgatc
    12841 tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt
    12901 acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt
    12961 ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc
    13021 ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa
    13081 aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt
    13141 gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac
    13201 ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt
    13261 caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt
    13321 ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg
    13381 tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct
    13441 tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt
    13501 catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt
    13561 tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact
    13621 cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct
    13681 ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat
    13741 ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg
    13801 aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtgcttg ctgtaaagct
    13861 tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct
    13921 gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt
    13981 actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct
    14041 tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag taaatccaat
    14101 acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt
    14161 ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa
    14221 tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg
    14281 aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg
    14341 attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct
    14401 agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga
    14461 ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg
    14521 tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac
    14581 ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca
    14641 aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta
    14701 gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct
    14761 tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa
    14821 cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc
    14881 gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt
    14941 attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt
    15001 gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt
    15061 tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac
    15121 ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt
    15181 catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat
    15241 acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg
    15301 atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag
    15361 aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga
    15421 gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt
    15481 tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct
    15541 gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa
    15601 aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt
    15661 tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta
    15721 tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa
    15781 tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt
    15841 gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt
    15901 gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt
    15961 gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg
    16021 cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat
    16081 ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt
    16141 cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct
    16201 gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg
    16261 ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg
    16321 tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa
    16381 gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt
    16441 ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt
    16501 tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat
    16561 cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct
    16621 gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca
    16681 ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca
    16741 ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat
    16801 ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga
    16861 tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta
    16921 aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct
    16981 cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat
    17041 tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt
    17101 ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt gccagccatc
    17161 tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc
    17221 atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc
    17281 gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag
    17341 tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc
    17401 taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc
    17461 gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt
    17521 tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat
    17581 caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga
    17641 ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt
    17701 gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt
    17761 tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc
    17821 gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt
    17881 gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt
    17941 atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc
    18001 aaccacagcg gccaattcta agacatctga tcttttttga taataagcag catgtacttt
    18061 ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct ccctcctttg
    18121 cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct
    18181 gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct
    18241 cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag tagcgtttga
    18301 aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa
    18361 tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca
    18421 atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg
    18481 taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc
    18541 aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg
    18601 actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg
    18661 aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc
    18721 aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc
    18781 cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt
    18841 ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca
    18901 cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc
    18961 gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg
    19021 ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat
    19081 caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga
    19141 ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga
    19201 cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt
    19261 tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta
    19321 atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt
    19381 cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt
    19441 tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg
    19501 cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag
    19561 catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt
    19621 aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa
    19681 caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt
    19741 gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt
    19801 gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc
    19861 attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg
    19921 ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac
    19981 ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct
    20041 tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta
    20101 ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg
    20161 gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt
    20221 aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa
    20281 accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc
    20341 cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc
    20401 ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc
    20461 atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat
    20521 aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt
    20581 ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact
    20641 actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat
    20701 ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa
    20761 atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc
    20821 aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc
    20881 ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg
    20941 caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa
    21001 aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat
    21061 ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt
    21121 tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag
    21181 ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat
    21241 acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt
    21301 cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga
    21361 ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa
    21421 cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca
    21481 aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa
    21541 ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta
    21601 atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa
    21661 tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa
    21721 gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct
    21781 gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt
    21841 tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt
    21901 ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct
    21961 tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca
    22021 gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt
    22081 tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt
    22141 gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag
    22201 aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa
    22261 aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga
    22321 ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc
    22381 ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg
    22441 tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt
    22501 gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt
    22561 tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt
    22621 ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc
    22681 taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt
    22741 cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca
    22801 gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt
    22861 taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt
    22921 gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac
    22981 ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt
    23041 tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag
    23101 taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat
    23161 tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac
    23221 aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg
    23281 ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag
    23341 tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc
    23401 atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca
    23461 atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg
    23521 cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt
    23581 aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct
    23641 gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca
    23701 tctgggtttt tcttgtagtt tactcgggta ccctctttct tcagctgttc cactaattca
    23761 aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag
    23821 gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct
    23881 ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac
    23941 tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt
    24001 ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg
    24061 aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc
    24121 cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa
    24181 agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct
    24241 tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt
    24301 aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata
    24361 aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca
    24421 gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata
    24481 tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca
    24541 tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac
    24601 ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc
    24661 ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc
    24721 ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct
    24781 gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg
    24841 gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt
    24901 aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag
    24961 cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca
    25021 tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag
    25081 gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa
    25141 tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt
    25201 gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca
    25261 ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc
    25321 aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc
    25381 gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag
    25441 atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt
    25501 ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac
    25561 tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa
    25621 acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat
    25681 gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc
    25741 cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat
    25801 gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca
    25861 ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc
    25921 gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac
    25981 attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac
    26041 caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc
    26101 caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt
    26161 tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat
    26221 ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact
    26281 attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt
    26341 tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga tacttatctc
    26401 taaaacataa atagaataac agacatgtct actggttttg ttttattcga ggaattattc
    26461 aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca
    26521 ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc
    26581 gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt
    26641 agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt cacaacctct
    26701 tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca tttgcgcttc
    26761 aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg gttccatgtc
    26821 acgataacgt tgcataccag taccagctgg gatgatctta cctataataa cattttcttt
    26881 caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt
    26941 ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt ttgtgatacc
    27001 tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat tgtagttttg
    27061 atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat cacctggatc
    27121 cattacacga actttacgca gcatttgacg aaccattact tcgatatgct tgtcgccgat
    27181 ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt tttcaacaga
    27241 taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg tcaatggtgc
    27301 accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag gaacagtata
    27361 cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg caggatcttc
    27421 tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt tcggattacg
    27481 tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc cggcaacccc
    27541 acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg attgagcggc
    27601 gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta
    27661 acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga ttgtcactgt
    27721 ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc
    27781 aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac gtcccactaa
    27841 acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga tttcaagacc
    27901 tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt caaccagacg
    27961 acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc ctttacgagc
    28021 cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt tcgaaatgat
    28081 tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc cggcaagctg
    28141 tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga ttggattttt
    28201 cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat tccaaacagc
    28261 aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt gttttgtaat
    28321 agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca gtacactgat
    28381 gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta agtctttcat
    28441 acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt ctgcgatgat
    28501 gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga tatgtgcagg
    28561 aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca agttgaagtc
    28621 tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga tgatcttacc
    28681 aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt cacctaatga
    28741 gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac
    28801 ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt cttccattgt
    28861 caagtagtag ttacctagaa ccatatcttg ggaaggagta acaactggtt taccatcttt
    28921 agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt gtgcttcttc
    28981 attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat aagcttcaca
    29041 tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga atgcttggat
    29101 accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct tgattacgtc
    29161 ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg catttttgat
    29221 gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga ataattcaat
    29281 ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa caacgataac
    29341 ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac gaccttgttt
    29401 ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag taactggacg
    29461 tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac gtttttcatt
    29521 ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt tattacggtt
    29581 gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac cttctaattg
    29641 aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca tccagcttgg
    29701 ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg cacgtgtacg
    29761 tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg catctagatc
    29821 aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga attcttgtcc
    29881 atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt tctctaaatt
    29941 cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt ccaaagcgcg
    30001 tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt accagatatg
    30061 tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt tcgaacgagt
    30121 aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt tgtattttcc
    30181 gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg
    30241 ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac cgtaagacca
    30301 gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt tatttacatc
    30361 gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc tgatcttcga
    30421 ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt caaagttttg
    30481 gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta attctttggc
    30541 agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc
    30601 gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca tgtccagacc
    30661 tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg gttttggaat
    30721 tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt catctgattt
    30781 gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg cccatacttc
    30841 catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt gttgtgtaac
    30901 caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt gagcaagttt
    30961 gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg tacgtccgtc
    31021 gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt cccatacgtc
    31081 ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact gacgtgcagc
    31141 catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca cccctaaagg
    31201 attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt cttccggcat
    31261 aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc cttcatggat
    31321 tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg acaattcatc
    31381 tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc catgtggtac
    31441 acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat gcaatagacg
    31501 ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc
    31561 atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt ttaatgcgtc
    31621 ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc
    31681 ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta ccaaacgacg
    31741 gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac caactaaaac
    31801 gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca gagtatcccc
    31861 tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg ttccggagtt
    31921 tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg
    31981 gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca aagcagctcc
    32041 tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt gaggattgat
    32101 caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg agtcatcgtt
    32161 ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg atacgtccat
    32221 gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag ccataacaac
    32281 gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta cgatgtaatg
    32341 gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg tttcacggtc
    32401 tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat aactggacaa
    32461 actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca tacggccata
    32521 gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca atccgccagg
    32581 tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg tttgatccat
    32641 gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa ctggacggat
    32701 attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc tttcacgaac
    32761 cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt caccgactga
    32821 acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac cttccatcaa
    32881 gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt ttacagctgc
    32941 ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg gtgaaaatac
    33001 tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg gatagtaagt
    33061 cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt ggtgagtcag
    33121 aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg tttcagctaa
    33181 tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt aacgaccaac
    33241 gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct
    33301 gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca aaccttcttc
    33361 tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc gtaatgtttc
    33421 gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg cacgaactaa
    33481 tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt cttttgcatc
    33541 agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga atccttcttt
    33601 tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta actgagaaac
    33661 gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta atgggaaatc
    33721 accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca aacgcaatgt
    33781 tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta ctgtatactt
    33841 tggttctttc aattcataat ctacaaattc caaagataga ttgccattga agtcatcgat
    33901 tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt ggtaagagtc
    33961 tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg cgaaacttct
    34021 acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca cccctctaaa
    34081 atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat attattttta
    34141 ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat agaacattct
    34201 cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc cgaaacggac
    34261 aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata tactacactt
    34321 attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa cattttctta
    34381 atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga cctttttatc
    34441 tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc aacatcaacc
    34501 aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag gtttgtattc
    34561 atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa agaactttgt
    34621 attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa aatacaaagt
    34681 aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc catttggaca
    34741 ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa
    34801 tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg cacaaaaaga
    34861 caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac ttgtacaaca
    34921 taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag aagggtataa
    34981 agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag acaacctgaa
    35041 aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa tcgccactcc
    35101 agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca cagcggtcac
    35161 agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca atgtgactgg
    35221 cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga
    35281 cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga tccaagctga
    35341 tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc
    35401 cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg gaatctatat
    35461 tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac
    35521 aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt
    35581 aggaatcgac tacgaaagct tgggtaaaca aacagggatc atggcagcaa aaatcttaga
    35641 cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa
    35701 taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag ctgaataaaa
    35761 cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc atcgcaaggg
    35821 gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt ggacattgcc
    35881 gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt aagtattaca
    35941 aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc ggcagcaggg
    36001 cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc agggatcatc
    36061 accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta
    36121 ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa agacaacgct
    36181 gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt tttgttctta
    36241 cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat gagcgaagca
    36301 aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa tggatgcatt
    36361 gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt
    36421 ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt acgtaaccag
    36481 ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta
    36541 gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc tgctttcgtg
    36601 ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag
    36661 aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat catacctttg
    36721 aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc ttagatcccg
    36781 gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg ctgaacagca
    36841 tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa aatatcacga
    36901 aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat cctaaactag
    36961 ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa
    37021 aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa gaccagctgg
    37081 ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag
    37141 gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa ctgatcttat
    37201 tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg gaattgacag
    37261 aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac atggaagacg
    37321 cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc
    37381 agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc aaacaaaaca
    37441 gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa agaggatggg
    37501 acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt tttgctttat
    37561 gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg atagaatgga
    37621 tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta ttgaaaaaga
    37681 caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc cggacaagta
    37741 tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac gggcctacag
    37801 ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa gagctacggt
    37861 cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg atgaaagatg
    37921 ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct gatacacaag
    37981 aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga ggaatctaca
    38041 tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat acgatcatcg
    38101 cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat
    38161 gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct gaagcagtca
    38221 gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt aatttttctg
    38281 ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc tttcccgacg
    38341 gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt
    38401 atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc gtattgggaa
    38461 aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg
    38521 atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct tctggcgaaa
    38581 tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga cacctgatct
    38641 catacagtgt ccttttttct gaattctctt caaaagactc tccttattct atcagctgac
    38701 agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat ggtacacttt
    38761 ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc aaaagagata
    38821 gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa aaacaaaaaa
    38881 gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat ctcttaatcc
    38941 caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat caataataga
    39001 aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta cttcatctag
    39061 ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta
    39121 tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca gactatgctt
    39181 ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt cttgactatg
    39241 ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc gagcggtatg
    39301 ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg gaaaacgatt
    39361 ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg cccgatccac
    39421 actctcgcat agcatatcta aataatccat ttattttcct cctttttatt tatcctactt
    39481 actttttcct aattctagga tacctactta tatgttacta cacaccctta ttttttacac
    39541 tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt gtcgaactat
    39601 caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat aaacaatgat
    39661 ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa tgtttgaaag
    39721 gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg ctttgttgtt
    39781 caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta aagaccctgg
    39841 gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta gtcaattcca
    39901 cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata tagcaacaca
    39961 tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat tgaaccaaga
    40021 aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac ctacaggtag
    40081 cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc aagatggtca
    40141 tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt ataaagatcg
    40201 tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg tagcagcaaa
    40261 tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc cagacatgaa
    40321 caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg taaatatcga
    40381 tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg atcaagtagt
    40441 tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa tcaaatacaa
    40501 tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt ggaattttgg
    40561 aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa
    40621 tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa atgtgatttt
    40681 agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca atggtacaga
    40741 tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct cagattcatc
    40801 a
//
LOCUS       DACCVU010000012        39434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCVU010000012 DACCVU010000000
VERSION     DACCVU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.012"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            129..1469
                     /locus_tag="IVW29_000546"
     CDS             129..1469
                     /locus_tag="IVW29_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838051.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1687..2130
                     /locus_tag="IVW29_000547"
     CDS             1687..2130
                     /locus_tag="IVW29_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7838052.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2634..4052)
                     /gene="nhaC"
                     /locus_tag="IVW29_000548"
     CDS             complement(2634..4052)
                     /gene="nhaC"
                     /locus_tag="IVW29_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7838053.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4455..5678)
                     /locus_tag="IVW29_000549"
     CDS             complement(4455..5678)
                     /locus_tag="IVW29_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7838054.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5709..6548)
                     /locus_tag="IVW29_000550"
     CDS             complement(5709..6548)
                     /locus_tag="IVW29_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7838055.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6565..7245)
                     /locus_tag="IVW29_000551"
     CDS             complement(6565..7245)
                     /locus_tag="IVW29_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838056.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7247..8320)
                     /locus_tag="IVW29_000552"
     CDS             complement(7247..8320)
                     /locus_tag="IVW29_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7838057.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8967..9638)
                     /locus_tag="IVW29_000553"
     CDS             complement(8967..9638)
                     /locus_tag="IVW29_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7838058.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9725..12403)
                     /gene="mgtA"
                     /locus_tag="IVW29_000554"
     CDS             complement(9725..12403)
                     /gene="mgtA"
                     /locus_tag="IVW29_000554"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7838059.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13062..13499
                     /gene="ndk"
                     /locus_tag="IVW29_000555"
     CDS             13062..13499
                     /gene="ndk"
                     /locus_tag="IVW29_000555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7838060.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13521..14387)
                     /locus_tag="IVW29_000556"
     CDS             complement(13521..14387)
                     /locus_tag="IVW29_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7838061.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKETSNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14547..16640)
                     /locus_tag="IVW29_000557"
     CDS             complement(14547..16640)
                     /locus_tag="IVW29_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7838062.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17389..18012)
                     /locus_tag="IVW29_000558"
     CDS             complement(17389..18012)
                     /locus_tag="IVW29_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838063.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18171..19568)
                     /locus_tag="IVW29_000559"
     CDS             complement(18171..19568)
                     /locus_tag="IVW29_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7838064.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19600..20571)
                     /locus_tag="IVW29_000560"
     CDS             complement(19600..20571)
                     /locus_tag="IVW29_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838065.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20945..21370
                     /locus_tag="IVW29_000561"
     CDS             20945..21370
                     /locus_tag="IVW29_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838066.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21858..22559)
                     /locus_tag="IVW29_000562"
     CDS             complement(21858..22559)
                     /locus_tag="IVW29_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7838067.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22575..23015)
                     /locus_tag="IVW29_000563"
     CDS             complement(22575..23015)
                     /locus_tag="IVW29_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7838068.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(23015..23590)
                     /locus_tag="IVW29_000564"
     CDS             complement(23015..23590)
                     /locus_tag="IVW29_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7838069.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24438..24917)
                     /locus_tag="IVW29_000565"
                     /pseudo
     CDS             complement(24438..24917)
                     /locus_tag="IVW29_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25076..25777)
                     /locus_tag="IVW29_000566"
     CDS             complement(25076..25777)
                     /locus_tag="IVW29_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7838070.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25885..27294)
                     /locus_tag="IVW29_000567"
     CDS             complement(25885..27294)
                     /locus_tag="IVW29_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7838071.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27342..29207)
                     /locus_tag="IVW29_000568"
     CDS             complement(27342..29207)
                     /locus_tag="IVW29_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7838072.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29328..30179)
                     /locus_tag="IVW29_000569"
     CDS             complement(29328..30179)
                     /locus_tag="IVW29_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7838073.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30489..31202)
                     /locus_tag="IVW29_000570"
     CDS             complement(30489..31202)
                     /locus_tag="IVW29_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7838074.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31270..31596)
                     /locus_tag="IVW29_000571"
     CDS             complement(31270..31596)
                     /locus_tag="IVW29_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7838075.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31719..31946)
                     /locus_tag="IVW29_000572"
     CDS             complement(31719..31946)
                     /locus_tag="IVW29_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7838076.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32268..33521)
                     /locus_tag="IVW29_000573"
     CDS             complement(32268..33521)
                     /locus_tag="IVW29_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7838077.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33582..34322
                     /locus_tag="IVW29_000574"
     CDS             33582..34322
                     /locus_tag="IVW29_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838078.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34408..35166)
                     /locus_tag="IVW29_000575"
     CDS             complement(34408..35166)
                     /locus_tag="IVW29_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7838079.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35163..36191)
                     /locus_tag="IVW29_000576"
                     /pseudo
     CDS             complement(35163..36191)
                     /locus_tag="IVW29_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(36211..39378)
                     /locus_tag="IVW29_000577"
     CDS             complement(36211..39378)
                     /locus_tag="IVW29_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838080.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
BASE COUNT        11236 a         8298 c         6740 g        13160 t
ORIGIN      
        1 aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc
       61 attgttaaat attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga
      121 ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt
      181 tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga
      241 tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga
      301 tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt
      361 tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta
      421 tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt
      481 tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca
      541 atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca
      601 gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca
      661 caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta
      721 tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga
      781 ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag
      841 gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt
      901 ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa
      961 tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat
     1021 ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa
     1081 aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt
     1141 tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat
     1201 tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca
     1261 gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct
     1321 tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg
     1381 gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga
     1441 atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg
     1501 gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt
     1561 ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat
     1621 aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg
     1681 ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg
     1741 tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga
     1801 gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg
     1861 aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt
     1921 gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag
     1981 gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat
     2041 cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa
     2101 atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat
     2161 gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata
     2221 aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
     2281 ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta
     2341 ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag
     2401 ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     2461 atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg
     2521 ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt
     2581 tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt
     2641 tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc
     2701 caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat
     2761 ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc
     2821 caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt
     2881 ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat
     2941 aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc
     3001 aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca
     3061 aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc
     3121 atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa
     3181 acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata
     3241 aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt
     3301 ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag
     3361 tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg
     3421 tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc
     3481 atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat
     3541 tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc
     3601 agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac
     3661 ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa
     3721 gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc
     3781 cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg
     3841 attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt
     3901 atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc
     3961 tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct
     4021 tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt
     4081 atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct
     4141 ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta
     4201 attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg
     4261 taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc
     4321 taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt
     4381 caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac
     4441 aagtttattt tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga
     4501 tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg
     4561 gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct
     4621 ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt
     4681 gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc
     4741 gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca
     4801 aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc
     4861 gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct
     4921 aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg
     4981 ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc
     5041 aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct
     5101 ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca
     5161 ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca
     5221 tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc
     5281 ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc
     5341 ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga
     5401 ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg
     5461 gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac
     5521 ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact
     5581 tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt
     5641 agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt
     5701 catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt
     5761 ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt
     5821 ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca
     5881 tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca
     5941 ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct
     6001 aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt
     6061 ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg
     6121 ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc
     6181 ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa
     6241 tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa
     6301 tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat
     6361 tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct
     6421 tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct
     6481 tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt
     6541 tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat
     6601 aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt
     6661 ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc
     6721 tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat
     6781 accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat
     6841 cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg
     6901 agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt
     6961 cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat
     7021 ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa
     7081 tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat
     7141 gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg
     7201 aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt
     7261 cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag
     7321 atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga
     7381 tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg
     7441 ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat
     7501 aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga
     7561 tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt
     7621 cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca
     7681 taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga
     7741 ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt
     7801 tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag
     7861 ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg
     7921 ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt
     7981 tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca
     8041 ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg
     8101 gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca
     8161 gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat
     8221 aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt
     8281 tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa
     8341 aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta
     8401 ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag
     8461 caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg
     8521 gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt
     8581 tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta
     8641 acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc
     8701 cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag
     8761 ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac
     8821 ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca
     8881 acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta
     8941 cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata
     9001 acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta
     9061 taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca
     9121 gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt
     9181 cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg
     9241 aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt
     9301 ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat
     9361 atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat
     9421 tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa
     9481 atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata
     9541 tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca
     9601 actaacaaac catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa
     9661 acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc
     9721 tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg
     9781 ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca
     9841 agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg
     9901 tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac
     9961 gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga
    10021 ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta
    10081 cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg
    10141 ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt
    10201 ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca
    10261 gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa
    10321 tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga
    10381 tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt
    10441 ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac
    10501 cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag
    10561 tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta
    10621 cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt
    10681 tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa
    10741 ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc agagctttca
    10801 acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca
    10861 tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga
    10921 caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt
    10981 ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca
    11041 gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca
    11101 caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga
    11161 tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt
    11221 tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat
    11281 ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt
    11341 tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca
    11401 ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg
    11461 gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc
    11521 aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt
    11581 tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt
    11641 cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc
    11701 cttccgcact tccactgatg acattacttc ccataaagac aagattttcg taacttgttt
    11761 cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg
    11821 tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta
    11881 tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct
    11941 ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt
    12001 cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca
    12061 tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt
    12121 ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg
    12181 ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt
    12241 aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata
    12301 gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt
    12361 tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt
    12421 ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag
    12481 ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt
    12541 ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc gaatagttat
    12601 ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa
    12661 aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag
    12721 atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac
    12781 cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt
    12841 tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt
    12901 cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt
    12961 ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc
    13021 tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta
    13081 ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag
    13141 caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag
    13201 aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt
    13261 ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga
    13321 tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc
    13381 tgccgggaac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga
    13441 tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat
    13501 acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct
    13561 aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag
    13621 tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc
    13681 aatgtttcat agatcactgt acttctttct ttatgtgata agctccgttt catatcttca
    13741 ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt
    13801 aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt
    13861 ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct
    13921 agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt
    13981 cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg
    14041 tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg
    14101 ctggtctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt
    14161 ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc
    14221 atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt
    14281 gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc
    14341 aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt
    14401 tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa
    14461 aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc
    14521 taactactga aggttggttt gattgtttat tgattatttt tttgctgttc aacgtctgtt
    14581 cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa gacacttgtc
    14641 gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt tttatagtca
    14701 tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa gagattttcc
    14761 tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc tttggagatt
    14821 tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt attgtaagct
    14881 tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa agcaactact
    14941 tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt atcatcgatt
    15001 cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc atgattgact
    15061 gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat ctgtgcgttg
    15121 tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc tttattatct
    15181 gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg gtaaatacca
    15241 gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg tgccgttcga
    15301 acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa attcttgtca
    15361 tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt
    15421 agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat cccataacct
    15481 aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt
    15541 ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta
    15601 tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc tgacccaagt
    15661 tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca
    15721 tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat cgttgcttga
    15781 ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc
    15841 aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca
    15901 aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc
    15961 ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccgtcataa
    16021 atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc
    16081 agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa
    16141 agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat aggtattttc
    16201 ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca gtagatgaca
    16261 tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac tttcgaaaag
    16321 cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat aatattcaag
    16381 tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc tggctttggc
    16441 tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg gttgatccac
    16501 aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata gtaggcaatg
    16561 atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac
    16621 aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt cttattttgc
    16681 tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt attctttcta
    16741 aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg attaaactaa
    16801 tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa gccagtttct
    16861 cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc ttatttcttg
    16921 atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg
    16981 ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc
    17041 tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccctc
    17101 ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt gttcaaaagc
    17161 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt ttttgaaatt
    17221 gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    17281 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
    17341 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc attgtccata
    17401 ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat tttcttcact
    17461 tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg actcgttttt
    17521 ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt ccgctaaacg
    17581 atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt ttccattttt
    17641 caaacgggct actttgtcgc caacatgagt caaaagcaaa acggtcaaat gcttgtcttt
    17701 aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt
    17761 ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat
    17821 tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt
    17881 gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa
    17941 tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct
    18001 ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa
    18061 aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac
    18121 aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca
    18181 aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt
    18241 ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac
    18301 gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg
    18361 gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc
    18421 atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata
    18481 aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata gattccttgt
    18541 tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg
    18601 aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc
    18661 cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc
    18721 aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca
    18781 cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct
    18841 atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga
    18901 ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac
    18961 gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct
    19021 ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta
    19081 aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag
    19141 gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg
    19201 aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga
    19261 tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc
    19321 cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct
    19381 tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc
    19441 ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt
    19501 ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat
    19561 tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat
    19621 attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag
    19681 ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag
    19741 gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat
    19801 tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac
    19861 aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct
    19921 agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt
    19981 ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat
    20041 ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt
    20101 cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat
    20161 actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag
    20221 cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc
    20281 ggcaaacccg cttaataata agctataccc attttcttca gctactcttt cgatccctga
    20341 gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct
    20401 tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc
    20461 ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac
    20521 tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca
    20581 ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt
    20641 tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc
    20701 tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt
    20761 ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt
    20821 tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc
    20881 atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag
    20941 tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg
    21001 ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt
    21061 aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat
    21121 ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta
    21181 tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat
    21241 caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac
    21301 aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta
    21361 cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt
    21421 aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta
    21481 catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat
    21541 gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct tatcctgttc
    21601 aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac tatggctgat
    21661 ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta aaacaaacag
    21721 tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa tatttgaata
    21781 taaaataacc aaacgataga aaggcttatt ctacatcacg tagtaatttt tctttcccgt
    21841 cgtttggttt tttattttta gcctaattct tgaccttttg catgctggtt atccgtaaaa
    21901 tattttccag caggctttcc ttccaattca gctaattttt cttggatctg acggatcacc
    21961 ttttttgcat cttctggatt ttctacaaaa tcgaatttat ccccatccac ttgtatttta
    22021 ggacatacat caaaatcttc ataccattga ttatagcggg tgttcaacgt ttcataatag
    22081 gtatataacg tctcatcgct ttctagctgt tcatagtcac gcccacgttt tttgatacgt
    22141 tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggccg tttttttgga
    22201 gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg ctgtacttcg
    22261 atatccgtta cccgacctaa atcagcgttt agatggaaca acaggctatc ttcatagatg
    22321 gatcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa aaatcgcttg
    22381 ttcaaaaaga agatttgcaa taaaaaagcg tactgctcag gatttgaata aaagagcggc
    22441 aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc ttttgcaagc
    22501 atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat cactgacata
    22561 caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa
    22621 tcatattcac gtacagcttg gtctgatttt aaatacgtat ggagactttg tgtaatcatc
    22681 aagttcgtag gtaccgtctc ttcttgtaca atgatcatcg gttttttcaa catagttgcc
    22741 acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc aaggatagca
    22801 acaacaaatt ctgcatttgt cagttcttcc atatcttttt cgtatacttc ctgcgcccat
    22861 ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta gaaacttgtg
    22921 actgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat ttgctcctct
    22981 gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc aataataact
    23041 gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg ttggaaaaga
    23101 ttacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt tctaataatt
    23161 gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggcttg tcgggcgtca
    23221 gaccgattgc tatccaagaa agaagttgtg agaattcctc caatgtacct agacctccag
    23281 ggaaaacgag gacagcatct cccttttcaa ataataatac ctttctttca tcgatcgttt
    23341 ctgtaggaat aaatgtagta acatcttttc caggcaattc agtttcaaaa agcccattcg
    23401 gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc actcccataa
    23461 tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccatg tattttccta
    23521 acgtttgcgc aataaatttg taactttctt tattaccaaa acgggaaccg caaaaaacag
    23581 taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta cacgtgtttt
    23641 tttaaaataa aagactagat tttgtagttt tttagtggta tataggatag acgccactat
    23701 atatagtatt tagaaaaaaa tcatttttca gccattttat agataaaaca aaaataaata
    23761 acgtgttttc atcgatatga aatgacttac ttatagaaga tactgggatt tcgtcctata
    23821 tgccaataac gtgtattatt ttaaacaaca aatacttgtt tcttttatta acgaaaaaat
    23881 cattttgttc tcgtgttatg tttcactgat aaatgatgtt tctctaaata aaaaagccga
    23941 cgaactaaac gatcaaaatt tgatcatttt tgttcatcag cttctcctaa aaatcttttt
    24001 gttaacgata aatagctgga tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa
    24061 attcagtgat aaccgcacct gttgcgcctg cctggaacta gtgtttgata ttgggctcga
    24121 acgtgtactg atctctagca tgaagaataa tttctttttt tactgtataa taactttctt
    24181 tcctttttgg aatagtacag gaaagttttt ttgtttcctt tccttcgaca taaggtatac
    24241 cagacccttt cgacaacgaa aggttctcat ttttccttca tcgtcatttc aaggagggtg
    24301 ccgatgctct ggacagtttc gatcgctcct aaaccaaaat ctgtatattc gattctttct
    24361 aactctgatt ctttcatacc attcctgtct caaggtacat ctattgtact tttttcttgt
    24421 aatccattct gcctctccta actaattgtc aaatcatccc aagatatgtc ttcttcctct
    24481 ttttcttctt ggatgatttc tgcattcgga tctactggtt ttttcaataa taaaatggtt
    24541 attcctgtga caaaagaacc agctaacaat gcaccaatga atgcccatgg tctggtcatc
    24601 gtagcgactg caaacattcc cgatgccgga atacggctat ctgcgcccca aaacatgctt
    24661 aatccgccac ctactgtccc accgactccc gttgctatga cattgcgtgc caagtcgttc
    24721 aatacgatag ggaaacatcc ctctgtgatc tgacagatcc ccataggaaa agcggtcttc
    24781 aatgtatcga tttcttgacg attgagtata tttttacgaa tgattttccc taagaaataa
    24841 gcgatcgtca agccgaatgg ggttaccatc gaagctaaaa tcaatacagt gattggttca
    24901 tagattcctt ctgaaaatcg tgccacaccc ccttatccag aataaatggt aatccagaaa
    24961 cagccgtgta tctgcggtaa taagcctaac caaacgaaaa atatgagaag ctatttttgt
    25021 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg
    25081 tttcataatc aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa
    25141 tcatttcccc aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg
    25201 ttttcagaat tccttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg
    25261 agtcaatagt atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt
    25321 ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat
    25381 tgttcaaata atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag
    25441 gatcttcctt ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc
    25501 gggagttctc caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat
    25561 tgcttagaac cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca
    25621 gttcgattgc ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt
    25681 ctgtcggcaa catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat
    25741 ctgcgacttc caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat
    25801 tactaaaaga atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg
    25861 ttctttttca ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt
    25921 ataccaatca aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag
    25981 atccacatag ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc
    26041 gatacatccc caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat
    26101 cgcttcgata tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc
    26161 ttccatatga tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg
    26221 attgtataat tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc
    26281 acttgcctca agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc
    26341 ttgtggatca gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac
    26401 tggatattct tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac
    26461 tccatatttt ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc
    26521 cgtgcagaaa aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt
    26581 ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg
    26641 caattcgtgg ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg
    26701 gaaaatattt tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg
    26761 aaagtgaaag gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta
    26821 tctctctaat actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca
    26881 gccaccgtat tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg
    26941 ttcaatatca tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc
    27001 gttaggagta ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata
    27061 acatttgaat cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc
    27121 gattcctcga tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc
    27181 actgccgata atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagtcc
    27241 ttttccgtct cgatcataag ccccttctaa ttgatttggc agcagttgct ccaccccata
    27301 aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc cttattttac aattgccaga
    27361 acatcttcgt ttgcaataat ctctttttgg tctaattcaa gtatatcggc aaatgcatca
    27421 gtattcgtaa tgacaactgg agtgattacg ctatagccag ctgctctaat tgcttcgatg
    27481 tcaaaacgaa tcaataaatc tcctgctttg acgagatccc cttgtttgac taagatctca
    27541 aagccattcc catttaattc tactgtatcc atcccgatat gaatcaaaat ctccgctccg
    27601 tcagtcgttg tgatccctac agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct
    27661 gcaggtgcaa ctagttctcc tacttctgga tcaatagcga taccttttcc catcacgcca
    27721 gaagaaaaaa cttggtctgg tacttcactc aatggcagta ctttacccgt taaaggactt
    27781 tttaaggtat gccgtgctga agtaatcggt tctcctgcat tcgcatgttt gttttctaat
    27841 tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca
    27901 aaagcaattc ctgtagctat tactgctact gtcacatttg actctacacc atccaccgta
    27961 ttaatgaaag tcggaatcgt caataagctg accaaactgc tggagaaagc ttttactccg
    28021 gaaaatccga taatagctcc accaatacct ccagaaatac aagcggcaat gaatggtttc
    28081 ttcaaaggta atgtcacacc atacacagtt ggttcagtga ttccaaataa cgaagtcact
    28141 gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa
    28201 gcagccccac cttgtgccaa gatggctgga agaagcattg gcatcaatac atcaaaccca
    28261 tattgttcga tattcaaaaa catgatcggt acgaatcccc aatgcatgcc gaacatcacg
    28321 aatacttgcc ataaacttcc catgattaga cctgctacga ttggactaaa tccataaatc
    28381 gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc taaagcaaga
    28441 aatgttaatg gaaccatcac tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat
    28501 tgcggaatca ctttttttac gaatggctct aatttacttt gaacccatac ggctagaatg
    28561 attggtatga cagaagaagt ataaccagat ggtgaaagaa tcactgggat cccaaagaaa
    28621 ctgatagctt ctcccgcacc agcaactgct gtgatagctg gatacacaag tgccatagca
    28681 attgctactg ctaaaaactg gttggctttg aactttttag ctgctgtaaa agcaagcatg
    28741 acaggtaaga aggtaaagac accatctgct gcagcaaaca atatttgata agtaccgcta
    28801 tcagctgtca gccagcccat caccgttgcc aatgataaga atcctttcaa taccccggcg
    28861 gctgccatcg cccctagaaa aggtgtaaat atactagaga taatatcgat cagacgattc
    28921 aaaatatttt tggactcttc ttttccatcc tccgaaactg caagaccttc ctcatccacg
    28981 atcgattgat aaacatctgc tacattactg ccaatcacta cttgatactg accgccactt
    29041 tgtacgactt ggatcacgtc cgggtcttct tttaactttt gcgtatctgc taaagattca
    29101 tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta aacttttgac attcttttgt
    29161 cctcctaccg cttcccatac acgacgacca actgcttgat tatccattgt tttttccttc
    29221 tttctttttt atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca aacattcgtg
    29281 aacctatttt ttcaatccag gttttgcctg ccatacagta acaatcctta tgattggatg
    29341 ctttttttct cacttactac tctttgaata tgaatcgtta aataaatctg ctctgcgatc
    29401 cccatttcct gtttttgcgt ttgtcgaaga tactcattga tccgttcgac acaatgaaac
    29461 gcttttggat actttccttg aactaatgcg tagagaaatt catcttgctc gtcagatcct
    29521 tcccctttga gataacgctg ggcaaaaaac tgcagatgag taatgatacg ctgataattc
    29581 aaagattctt catcgaacac gatgccgaag aatttactga tgatactcag tatatctctt
    29641 accattgtgg tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg gctattcaca
    29701 aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc
    29761 acgaacaaaa gagcctcttt cgctgcttga tattctttcg gataaaattt ttttgtttca
    29821 aataataatg gattaggcag ttggatattt tcttttgctc gtgtgatcgc ataatgaaga
    29881 tgatcggtca atgtcagata aatattaggc gaaaattcaa cttttagttt atcttcagcc
    29941 agttcgacta cttgtttgac ccattcgatc tcctctgccg gaatatcagc catcagctgc
    30001 tggaattggt ttttagcaaa agaatctctt aaaacaaatt ctttttggac cgcttctggc
    30061 gaaatctcgt ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct
    30121 tccccgaatc catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt
    30181 ttgcttcgcc ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg
    30241 aattctggtt ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca
    30301 tcgaattttt atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc
    30361 tatctagaaa acaaccaaat cagagatgca cctctttgcg tttctctttt tttcagcaaa
    30421 ttggtttttc cttatcgatt catatccttt ggctgttttt aatctgtaac gacttatgtc
    30481 ccagtatcct aaaatgtttt tgtctctaga ttgaaagcag atctcatatc atctgtcgca
    30541 tcttttatcg atgtaacact ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc
    30601 atgtcgccac aagtaattag tgtgacaatt tcttttccct cgacttcatt cagtaattgg
    30661 gtatctgtag gagcaacttt ttctttaaaa aatgttttgt atgtatacac attttctaaa
    30721 tctgtcaaat agatcttggc acccatctct aaattttcta gcggtgtaaa cagcaattca
    30781 gggttcgttg cacgatggct tgctaaggca taatttcctt ttcccatttc ttgatctgga
    30841 gataatgttc ctgctccgta aagtagtgac gtattatcca agcctttgaa aaccggtaaa
    30901 ttgattgaaa ctgaagggac tgcgatacta gcaatcactg gtagatctgt tcctctcatt
    30961 tgcgacctca tgaccccttc tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca
    31021 tctttatcgt tattcttttt cagatcttct tttgttacat tcgctatagc atatttatct
    31081 cctgtctctc tgacaaaata atccttgatc tgttcattga aaatcaaagc tagtcctact
    31141 aataacaaga ggaacaagag gatattgact agccatcttt ttgcttttcc tttttttttc
    31201 attttctctc cctccagact cattttactg tttttctttt gctctttcca acagccgaat
    31261 gacacatttc tacaactctg tgtatttttt tcgtacacct gcgctttttt ctacaggata
    31321 tttcaaatta tttttgatca atttttcttc aatgatctca tctagatcca ggtgaaggtt
    31381 gtctgccatc atgtatgaat agatcaatac atctgccagt tcttctttga ttcgttctaa
    31441 ttgtgtttct tttacttctt ccggcgtttt ccattgaaaa agttctaaca attcagatgc
    31501 ttccaaagaa atagagatag ccaaatcttt ttcgttatga aattgtcgcc aattacgttc
    31561 atctctaaac tggttgactt tttccatact attcatttgc ttgccttctt ccaatcactt
    31621 cttacttgat taatcatacc atttttttac ataaaaaata gatgatgctt ttaaaaaaac
    31681 tcctgttttc ttttatttaa aataaatgtc atagaaaatc aataatttag gtagccacca
    31741 tgtgtatgca gtagcgtgtc ttcttccctt gaggcttttt gtttttcgat acgttcttct
    31801 tcccaaacag acaacgctac tagaatctct cttaatgatt ttccttcttc ggtgagcgag
    31861 tactctactt taggcgggac ttggttatag gcttttcgat ggatactttc atcttgttct
    31921 aactctctta actgctgggt cagcattttt tttgtgatcg tagggatttg cttgcttctc
    31981 cacgcctaaa ataagcgata ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca
    32041 ttttgacgat cgtgggttcc gcttttcact tcttatctac ttagtatcat gaagtatact
    32101 ttcgtactaa tactaagtag cggaagtgtc aactactttg tttaactaca tagcaaaaga
    32161 aacagaaggg tgacaacggt catcacgctt ctgtttcttt tgaatatgct gtgttgttgt
    32221 ctctatctcc gtgtagactt tcctcaaact tatcctttga cagtaagtca gtaggctgat
    32281 ttgctctttc tagatgccag atacatttct acaaaaaaat agtgattctg ttttgaaaat
    32341 tcgattttta gttctgcatt ttcgtaaaca tccaacattt ttttcgcact gtatagacct
    32401 aacccgccat tttttttaga ggtttttcct cttttgacca tctccgatag cgtcaaattc
    32461 gttggatttg ccttgttcgc tatactaaaa cgtgatccct ctttttcttc cagcagattg
    32521 atcgtaatcg gactctgatc ggctacacga tgatcgaatg cgttagaaat aaaaatagat
    32581 aaacagcgaa taaaaacaac cagatcaatc gggattgttt tcacttcgac aggaataact
    32641 acttggatcg ggattttctc cgattctgct ttttctagaa attttaaaaa gacacccctg
    32701 acagctgggg atacaatatt tgttagctgt tgtatttgcg gtttttcaaa aatagctgca
    32761 gaatgatctt ctaatatttc taaaaattcc aacgtttctt ttttattttc ctgaagagta
    32821 atttttaatg aaaacagtag acttttataa tcatgtttga attcctctag atgatggtaa
    32881 tattcttcat ttatttgata cagttgaagc gattcctgat gttgtctttc ttttacttgg
    32941 aaactgatca acataatgga aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga
    33001 gatagaagca gataattaga tgagcggaag tgaacacttc ccaataccaa gtaaatcacg
    33061 ccccagctta ataaaataca aatacctcca aaatagtgtt cagttggata agtaaaaagc
    33121 aagttgagaa atctcgtctg tttgtctata ctttttacta atagatagaa aataaatgcg
    33181 ataagtagtg caaaaaaact tgctgttcca agatgtctct ttcctaaata atccatcaga
    33241 tcaaaaacca gaaaaaagct gagtccagtg atagcaaaaa aaagattact gatcaaacag
    33301 ctgataaaca ggtttttcgt agtaaaataa aaaagaaaca tcaataataa gccaactagg
    33361 taaggaaagc tcactttagg gataggaagt aaaagtgtca ccaccatcaa agctatacaa
    33421 ccaaagctgt tttttactag cagttgtttc gaaaagaata aatagatacc taaatctaaa
    33481 atcaaagtaa aggtcaaata aagtgtcact gttgcgacca tcttattcct ccattgtttt
    33541 gtttcaaaat gtacacatat ttttttaata ggagcctgca tatgaaaaag tattttatca
    33601 tagaagataa ccagtttcac agcgaatgga tcatcagaga attggtcaat catcatatcg
    33661 agaatatcgt gcagctcact tctatctctc aaatcaatca gttgatccag ttgggcacta
    33721 ttgataatca cgatgttttc tttatcgata tcgacttgaa gcaatcactc acaggtattg
    33781 atgtcgccga acaaatacgt aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt
    33841 atacgaattt tgcagtagag atcatcaatc ggaaaatcct tccgattgga tatatcagaa
    33901 aagacaggaa tttatctgaa caaattcgtc aagtgctaga acagatcaat tggaaagaac
    33961 agcagatcgt aaatgtcgat aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt
    34021 atcctcggga aattttatat attgaaagta tcaaaggttt gaaaaaccaa gttatggtca
    34081 aaacaactac agaagataaa ttagtgaata cttctttaaa acaattgaag acagagttag
    34141 aaaagtatgc ttacttttta cttttaaaat cttactgtat caacttatcg caattgcaga
    34201 aaattgattc tgctcattat gtgttagaat ttttcaacgg tgactcctta ctcgttggtc
    34261 gaaagatctt tgaaaagaca aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt
    34321 gatgaataag agctgagacg taagcatagc agatttcagg gagtgggcct tgaatcggct
    34381 ccactccctg aaatcgttga tatgatttta cgcttcctct tctgtaggag catctgttaa
    34441 cgaccatgtg aatgttgaaa cgtagttgtc tacagcataa ttggatacag gaacaaataa
    34501 ttccactgat tgtctccctt gttccacatt ctctcctaat gaataaatcc atgtgccaat
    34561 cccttcattt gctcctgcag ttactagatc tactgcctga tctgctggga tttcgattcc
    34621 ttcagcgcca gttccagaaa cggtagatgg cattccactg ctattagttg cgctatttcg
    34681 aatcacggcg tttttgaatt tcacctttga accagttagt ttattcgtat cagatgttgt
    34741 accaataaat tctgttcgtt tgactgataa tttccaaccg gcataatttc cgcttttatc
    34801 cgttacttgt acgaaattct gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg
    34861 taatgcagca tatactgttt ttgctgtacc taacttgatt gttccaaaat caagatctgg
    34921 agcttggtca atcgtcagta atccggatgt tccctgattt ggttcgtcag gtgtaaaagg
    34981 agtatcagga tcatttggat caacaggagg aactggaaca acgtcttcag gatttgcagc
    35041 atctggtttg gtcacagaaa tcgttccttc tgtagtggct gagctcttat tctcagcaaa
    35101 aacactttgt gtacataata atagacttcc agctagaacg gtacttaatg caattgtttt
    35161 tttcatttct ttttccttct ttcttttaaa ataaataatc cagcaaataa gccaataaga
    35221 atgactccaa caagtatcag ccatcgccag tcaaatgttt ctggctgatt ttttaaatca
    35281 atatcctttt cattcaattg gtccgctgca tcatcagtta tttcaaaatc ggtatggaga
    35341 ttccagcgtt tttctttcca agcacccttc acaaccgctt gataatgtcc cggagctaat
    35401 ttttttcctt ctaatgggac aagtaaatca aaatttgtat tgggtgctac atctattttt
    35461 tcttttttta ttgaaaggta aggctctttt gtttcttttt tgtagatttc aaccgatgta
    35521 gtcaagccga acatataaga agcatttgga ttttgaagat tcgccttaat cacatttctt
    35581 ccatcgcttt gatccgcata agcttttttt aatttcaaat ctggggaaga ctctttttcg
    35641 ctatttcaaa tcaagattgc tttcacttga gaaaactgat tgacgacacc aactgtactt
    35701 ttgttagaaa cgttctcttt tttttcttga aacgtgatcc ctcctgcgac cacaccagcc
    35761 agctcttttt ctggcgcttt cagtgaaagt gtctcagtga ccgcactttg aggaggaaca
    35821 gttatttctc ccactgtttt tgctaagttt ttcatatcat aacttaatgt ttcatcaaat
    35881 ttactttttg attctccata ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc
    35941 aatgtctcaa ctgttactga tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt
    36001 gttttcccag gctccatcct tacatttata tatgttttgt tcttatcaat ctgattagcc
    36061 ggtaattcaa aatctaccgc aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg
    36121 atgaatccaa ggagaaggat cataagtaaa gagcagttgt ttcgatgact tttattccat
    36181 attcgattca tcaatcatcc tccttgatct ttattctgtc ggcgcatctg ctaatgtcca
    36241 tgttatctct gctgagtaat taccagctat ctgattttct ttggttaaga caagtttaaa
    36301 cgcccgaccg ttatttaaat attctgaata atctgcttgc acattcgttg aattttgtcc
    36361 tctgacaatt tccagttctt cttcattcac taattgatac tcctctcctt tcttgaaata
    36421 taggacatcc tgcatagaaa atcctgcatc atttgtgaaa ggctgagata attttatcgt
    36481 taagatccac ggatttttcg tttctcgctg atcataaatc cataattttt cctgtatatc
    36541 catttggcca aatgcttctt gtgtcaaacc agttgctttg atctgaccaa aatcgagttt
    36601 ggaaggggca agaaatgaga gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg
    36661 cggggcatca tgataaacaa ctggcgtatc acttggattg tgattcccaa tttcatcgtt
    36721 ggtacttggc ttgttctgca cacccttagc agtagcttct cctgcttggt cgtttaatac
    36781 aatttctaat acatctcctg caaaaagttg attttctggg atagaaagaa caaattcccc
    36841 tgtatcatca atagttgttt ctgacaaaac agcttgattt tttttcaatg aaaccaaggc
    36901 tcctgcttca cctgtacctg tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa
    36961 attagcaggt tcaggaggaa tcacatcgac tgtcgttttg gcagagtcca gttctgtcac
    37021 ttcttggcca cctcttactt ttttagcagc aacaacttgc acttgatcct ccgtttttaa
    37081 tctctcatca ttgggtactt gaacttcgaa caagccgcct tgttcgcctt ctccccatgg
    37141 gttatatcct gccccttcat tttcatctgt ttgtccttgc gtgagagcag taatttctac
    37201 agatgatttc gtcgtatctg cgtaagtaat acgtaaagtc acttctactt cgcctgtcca
    37261 agcatctctt ggctttgcct cgacaccttc gggaacaacg acatgcccat agattttttt
    37321 atctgcattc gttggcactc ttaaggattc aatcatcggg agctgattat tggcactgat
    37381 tcgattataa tttttcattc cggcacctgt ctgagcaaat tcatttgtta aacctgcact
    37441 agggcttgat acgaaattgc ctaagtcggt cccattgatc gataagtctg tcatatcaga
    37501 aaaataatcc gggtcttttt caaagttatc gaatgttgcg cctgtttttc gccataaagc
    37561 gatttctgaa tgggcgatct ttaaagaaga atctccattt ccttggaata cccagccgcc
    37621 tatgccattg gtctcgcttc caatccgttt gttttggaaa tcaaaatagg taggagattc
    37681 taagtccaca ttcaacacat ccccactgat gatgccggtc ttatcttccc ctgttttccc
    37741 gaccatgact aaataagttt tttcacctgc cgtaaaattt aaatcaatat ctccagttgt
    37801 atctacagtt gctccggaat ctgcgcgtat atcgatattt gatttttctc cagtgactgt
    37861 aaaatcagct gaacctggac tttctagaaa accagaagcg tcataaaatt ggatcgcttg
    37921 atttccttga ttgagtctgt tcacgtaaga tttgccatca cctgtatttc ttaccctcaa
    37981 tgagcctcca ttggttactg tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga
    38041 acgcccactc tgcttatcga tgtcgataac tgatccggct ccttctacga tcaaagaaag
    38101 gccttttcct acaaaacgca tcccattgtt cccgggttga tcgtcatctc cagccatttt
    38161 gaccgagatt tgtccaccac ttgatacatg gatttgacta gaatctcctt gcaagcgaaa
    38221 tccgcccgta gttgctgtat tgatggtgat tttcccacca ttcgatacag tgactttcga
    38281 tcctgaacct ctcatttgga tgattccgcg attgttgtca ttggtaatat tgatctttgt
    38341 atttaccgaa aattcgcttc ccgaaccact gacttcaaac atgacattac caggtagcgt
    38401 atcgactgcc tctccttcat cgctaacgcc atctgcccga tcataatcaa gagagacctt
    38461 tgaaccgctt aagatatgga ttcctggcgc tgtttttcca ctaccagtct catctcctga
    38521 atctagaaaa ccgtagaatt tccctagata cggtccataa agttgactac cattgatcac
    38581 attcaattga tagcttctac aaaaataggc tttacccgga gcggcattca gtccttcctt
    38641 cactggttgg acatccaaca caccggaaac accatctaat gtgacagttg cacgaggagc
    38701 gtaaatcagt cctaatttgg acccatcata caaatcaagt gtcccggtaa attttatttc
    38761 tcccgtaaaa tgatatctgc ttccccaaaa atcggtcata taagcaatga taatggcatt
    38821 tccatcgata tttggtgtca atccacctgg atgtgcagct gttcctttaa aattctggaa
    38881 ctcgatcgac tgaggtatct cattgatata aatatggtgt gtgccgaaat tgtgagcgaa
    38941 tccaccaaaa tcaataacga tatctcgttt gatctctaat tccctgtcta aagtcaaagg
    39001 ctgaatcagt cggatcttag ttactgattc gtctgcgaga agctgcgcca gttcttctgt
    39061 tgtggcagct tcgctgtacc catccgctga aacagctctt gccagtgccg cttcactgct
    39121 atcagacgtt tgttcagcta cttctgaggt ctcacttgtc ttattttctt cttgcctttt
    39181 attagtagaa gcctcacttt gagattctgt agtttcagaa gtaatcgatt cttctgaaaa
    39241 agtagtcttg tcaaaacttt gattctctga ttgcgttgat tcgtttgttt tctctacatc
    39301 ggcaaaaacg gatgtcacca tcaagagagg gaagacaaac aaaatcgacg caatcagctt
    39361 ttttacacat tgattcattc tccaccatcc tctctgacgt tttgacaatt ttattttatc
    39421 gttttaatct attt
//
LOCUS       DACCVU010000013        38458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCVU010000013 DACCVU010000000
VERSION     DACCVU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.013"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            151..798
                     /locus_tag="IVW29_000578"
     CDS             151..798
                     /locus_tag="IVW29_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7838081.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            813..2339
                     /locus_tag="IVW29_000579"
     CDS             813..2339
                     /locus_tag="IVW29_000579"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7838082.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="IVW29_000580"
     CDS             complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="IVW29_000580"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7838083.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5448..6155)
                     /locus_tag="IVW29_000581"
     CDS             complement(5448..6155)
                     /locus_tag="IVW29_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7838084.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6176..6958)
                     /locus_tag="IVW29_000582"
     CDS             complement(6176..6958)
                     /locus_tag="IVW29_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7838085.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7007..7294)
                     /locus_tag="IVW29_000583"
     CDS             complement(7007..7294)
                     /locus_tag="IVW29_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7838086.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7309..7908)
                     /locus_tag="IVW29_000584"
     CDS             complement(7309..7908)
                     /locus_tag="IVW29_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7838087.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7922..8599)
                     /locus_tag="IVW29_000585"
     CDS             complement(7922..8599)
                     /locus_tag="IVW29_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7838088.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="IVW29_000586"
     CDS             complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="IVW29_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7838089.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="IVW29_000587"
     CDS             complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="IVW29_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7838090.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11361..12581)
                     /locus_tag="IVW29_000588"
     CDS             complement(11361..12581)
                     /locus_tag="IVW29_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7838091.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12596..13684)
                     /gene="murG"
                     /locus_tag="IVW29_000589"
     CDS             complement(12596..13684)
                     /gene="murG"
                     /locus_tag="IVW29_000589"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7838092.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13710..15071)
                     /locus_tag="IVW29_000590"
     CDS             complement(13710..15071)
                     /locus_tag="IVW29_000590"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7838093.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15085..16047)
                     /locus_tag="IVW29_000591"
     CDS             complement(15085..16047)
                     /locus_tag="IVW29_000591"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7838094.1"
                     /translation="MEWTQALIPIVSSCAMTISAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16073..18265)
                     /locus_tag="IVW29_000592"
     CDS             complement(16073..18265)
                     /locus_tag="IVW29_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7838095.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="IVW29_000593"
     CDS             complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="IVW29_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7838096.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="IVW29_000594"
     CDS             complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="IVW29_000594"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7838097.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="IVW29_000595"
     CDS             complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="IVW29_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7838098.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20253..20621)
                     /locus_tag="IVW29_000596"
     CDS             complement(20253..20621)
                     /locus_tag="IVW29_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7838099.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20792..21748
                     /locus_tag="IVW29_000597"
     CDS             20792..21748
                     /locus_tag="IVW29_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7838100.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21821..21937
                     /locus_tag="IVW29_000598"
     CDS             21821..21937
                     /locus_tag="IVW29_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7838101.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21992..23674)
                     /gene="recN"
                     /locus_tag="IVW29_000599"
     CDS             complement(21992..23674)
                     /gene="recN"
                     /locus_tag="IVW29_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7838102.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23692..24141)
                     /locus_tag="IVW29_000600"
     CDS             complement(23692..24141)
                     /locus_tag="IVW29_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7838103.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24248..25066)
                     /locus_tag="IVW29_000601"
     CDS             complement(24248..25066)
                     /locus_tag="IVW29_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7838104.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25075..25965)
                     /locus_tag="IVW29_000602"
     CDS             complement(25075..25965)
                     /locus_tag="IVW29_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7838105.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25967..26191)
                     /locus_tag="IVW29_000603"
     CDS             complement(25967..26191)
                     /locus_tag="IVW29_000603"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7838106.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26196..27533)
                     /locus_tag="IVW29_000604"
     CDS             complement(26196..27533)
                     /locus_tag="IVW29_000604"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7838107.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27534..28388)
                     /locus_tag="IVW29_000605"
     CDS             complement(27534..28388)
                     /locus_tag="IVW29_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7838108.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="IVW29_000606"
     CDS             complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="IVW29_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7838109.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28927..29352)
                     /locus_tag="IVW29_000607"
     CDS             complement(28927..29352)
                     /locus_tag="IVW29_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7838110.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29598..30662)
                     /locus_tag="IVW29_000608"
     CDS             complement(29598..30662)
                     /locus_tag="IVW29_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7838111.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30834..31079)
                     /locus_tag="IVW29_000609"
     CDS             complement(30834..31079)
                     /locus_tag="IVW29_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7838112.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31218..31592)
                     /locus_tag="IVW29_000610"
     CDS             complement(31218..31592)
                     /locus_tag="IVW29_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7838113.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31675..32589)
                     /locus_tag="IVW29_000611"
     CDS             complement(31675..32589)
                     /locus_tag="IVW29_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7838114.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32610..33413)
                     /locus_tag="IVW29_000612"
     CDS             complement(32610..33413)
                     /locus_tag="IVW29_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7838115.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33446..34435)
                     /gene="manX"
                     /locus_tag="IVW29_000613"
     CDS             complement(33446..34435)
                     /gene="manX"
                     /locus_tag="IVW29_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7838116.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34538..35029)
                     /locus_tag="IVW29_000614"
     CDS             complement(34538..35029)
                     /locus_tag="IVW29_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7838117.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35220..38054)
                     /locus_tag="IVW29_000615"
     CDS             complement(35220..38054)
                     /locus_tag="IVW29_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7838118.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT        10754 a         8358 c         6505 g        12841 t
ORIGIN      
        1 acgaaagaat gagtcttctc tatcatcttt cgttttttgt tgtaaaaacg tctaaaaatc
       61 ctttgttttt tatttagaaa aatagtcaat taacgttttt tttgttacac ttactaatga
      121 tggtttttta ccatgaaagg atgatcgaat atgacaccaa atcgagaaga ttatttgaaa
      181 atcattttag aactaggtgg agatacaacg aaagtcaaca ataaacaaat cgtatctagt
      241 ctagatgtct cagctgcatc tgtcagtgaa atgatttcta aacttgtgaa agaagagctt
      301 gtagaacatt ccccttatca aggtgtacaa ttgactgcaa gcggactgca aaaagctagc
      361 agtctgattc gcaaacatcg cttatgggaa gtttttttag ttgaacattt agactatagc
      421 tggaatgaag tccatgatga tgctgaagta ttggaacatg tgacttcgga acatcttgca
      481 gatcatttag aaaactattt gaatcatcct gaacattgtc ctcatggagg atcgattcct
      541 aaaaaagagg aagttgtcca tgaagaacgc cgacaaacat tggagtccta tcctgtcggt
      601 acaaaagtgc gtatcgcacg agtgcttgat gaaaaagaat tgttggatta cttagtcact
      661 attgacctga atatcaacga agaatatgag atcgttgaca tcgcagctta tgaaggaccg
      721 atcactatcc aaaatgatag aaagaaaatc cccgtcagct ttaaggcggc cagcacgatt
      781 tttgtcgata aaatataaag gagtgtatcc acatgtctaa tgaaaaaaat gtattgttta
      841 cgatattcgg cggtacaggt gacctagccc agcgtaaatt gtacccgtct ttgttccgcc
      901 tttacagaaa aggaaattta ggcgagcatt ttgctgtaat cggaaccgct cgccgtccct
      961 ggagcgatga gcactaccgt gaagttgtca aagaaacaat ccgtgcattg aatccttcag
     1021 atgaagaagc aactaccttt gcaagccatt tttactatca atcccatgat gtaaatgatt
     1081 caacgcatta tcagacatta aaagaattgt ctgaccgatt agatggacac tatcaattaa
     1141 acggtaaccg tgtctattat ttggctatgg cacctcaatt ttttggaacg atcgtttctc
     1201 atttgaaatc acaaaatatt atgacaaaaa atggcttcaa tcgtttgatc atagaaaaac
     1261 cgttcggttc tgattaccag tctgcttttg aattgaacca gcaaatcaga gaagtattcc
     1321 cagaacaaga tattttcagg attgatcatt atttaggaaa agaaatgatc caaaacatct
     1381 cagctatccg ttttgccaat aatatttttg aggctcaatg gaacaatcgc tatatcgaca
     1441 atatccaaat tacttttggc gaaaacctcg gtgtagaaga tcgcgggggc tactacgatc
     1501 atagtggcgc acttaaagac atggtgcaaa atcatatttt acaggttgta gcattacttg
     1561 caatggaacc gccagtagca ttttccgaaa aagaaatccg tacagaaaag atcaaagcat
     1621 tgaaagctat ccgcatttat aacgaagaag aagtgttaga aaacttcgtt cgcgggcaat
     1681 atgctcaagg tgaattggat ggtgtacaat tcaagggcta tcgcgaagaa gacaaagtgg
     1741 atgctcaatc agagactgaa acattcgtcg caggcaaatt cactatcgat aacttccgtt
     1801 ggtcaggtgt tcctttctat gtacgtacag gcaaacgttt gactgaaaaa gggactcgga
     1861 tcaacatcgt attcaaacaa gttcctgtta atgtattcaa aacatctgtc gatgaaccat
     1921 gtgacgatac gacacttcca cctaatgttc taacgatcta tattcagcca actgaaggat
     1981 tttctttatc cttgaatggg aaagaagtag ggcaaggatt tgaaacggaa ccaatcaaat
     2041 tggaatttag aaatagtgca gaaatggttg aaaatagccc agaagcttac gaaaaattac
     2101 tattagatgc gttgaatggt gatggaacga acttctctca ttgggaagaa gtcgctcaat
     2161 cttggcatat cgtggatgtc attcgaaaag cttgggacca aacacaaccg gattttccca
     2221 actacaaagc tggatcaatg ggacctaaag cagctttcga actacttgaa aaagacggat
     2281 tcgaatggat ctggcaacca gataactggt atcgtgaacg tggaaaatta aacgattgat
     2341 aacaaaaggg ggtgtgacaa agaacatgtc acaccccctt ttccctttta cttttcttcg
     2401 aatccttcag ctacagcttc aggatagaag tcttcaacga tttttgcaca acgaccgcat
     2461 agatgaggaa gtttttcatc tactcctaca tctttacgaa cttggcgaca gcgatcgcaa
     2521 gtttccccgt ctgctttttc cactgtaata gcaacatcat caaataccat agcgttatct
     2581 ggcacttgtt catctgctgc cataacttca aagtagtctg gtgaaacgat caataattgt
     2641 gcaatatctg catccaaagc ttttaacatt tcacgaactt gttgttttgg ataaacagtt
     2701 agttttgctt ccaaagattt accaatcaat ttgctgtttc ttgcttcttc caatgctttt
     2761 aagacattat cacggaagtc catgaaagca gtccaggtat ccatcaattc ttcttggttt
     2821 gcaaactctt cataaccagg aaactcgctt aactgaacgt actcctcttc ttctttcagg
     2881 aatgaccaga tttcttctgc tgtatgcgga atgataggag tcaataattt tgtcaaagct
     2941 acagctgttt gatagaaaac agtttgcatg caacgacgtt gataatcatc ttctgcttca
     3001 atataaacta catctttagc aaaatccagg tagaatgacg atagatcaac cgtcaagaag
     3061 ttcataactg tacggtaaat atgcatgaag ttatatttgt cgtacccttc ttctcggatt
     3121 tctttgatta cttggttcaa tcgaacggtc atatatttgt ctacagaacg taaatcattg
     3181 tacgaaacag tatctttttt aggatcgaaa tcttctgtat tcgctaataa gaaacgcatc
     3241 gtatttctga ttttacgata tacttctgcc acttgactta agatatccat agaaacacga
     3301 acatctgctt cataatctac actagatacc cataagcgaa ggatatctgc accaaattgt
     3361 ttgatcactt tttctggtgc aatgatgttt cctaaagatt tactcatctt acggccttca
     3421 ccatctaaag caaatccttg agaaataact gatttgtatg gtgctacacc attgatagcc
     3481 acactagtcg tgatacttga gttgaaccag ccacgatatt ggtctgatcc ttctaaatac
     3541 atatctgctg ggaatgataa ttctggtcgc tgacgtaaaa cagcttcatg agaagaacca
     3601 gaatcaaacc agacatccat gatatctttt tcttttgtaa attcgccatt aggagaacct
     3661 gggtgggtaa atccttcagg aagaagttct ttcgcttctt tttcaaacca gataattgat
     3721 ccatgttcag caaataattt tgccacatgt tcgattgttt ctggtgtgat gattgcttct
     3781 ccattttcag catagaaaat cggaagtggt actccccatg cacgctgacg ggaaattacc
     3841 caatcgccgc ggtcacggat catattatac aaacgtgttt taccccatgg gataacccac
     3901 tctacttttt ctacttcatc caaaatattc tgacggaatt tatcgataga tgcaaaccat
     3961 tgtggagttg cccgatagat cacaggcttt ttcgtccgcc aatcgtgagg ataactatga
     4021 gtaaagaaat ctagcttcag caaagctcct ttttctttta atagatcagt gatcatagga
     4081 tttgctttgt cataaaagat accttcaaat ccaggtgctt cttctgtaaa caatcctttt
     4141 gagtctacag gtgacactac agatagttta tatttgctac cagcgatata gtcatcttcc
     4201 ccatgtcctg gagcagtatg gactaatcca gtaccagcgt ctaaagtaac atgatcgcct
     4261 aaaatcacaa gtgattcacg ttcatagaat ggatgacttg cagtcatata ttccatttct
     4321 tgtccagcaa attcttttag aactgttaca ttttcccaac cgatttctga ttggactgtt
     4381 tctagcaaat ctttcgctac gacaaactta cggccatcgg cttccaccaa tacatacgta
     4441 aattttggat ttacagcaat accttggttt gctggcaatg tccatggtgt tgtcgtccaa
     4501 atgacaaaag ctgtatcttc atcaagaata tctttgccgt ctttgacatt gaaagctaca
     4561 aaaatagatg gagattttac atctttatat tcaatttcag cttccgctaa agaagactca
     4621 cttgacggag accagtaaat tggtttcaat cctttgtaaa tatagccttt ttcagccatt
     4681 ttaccaaata cgcgtacttg tgcagcttca taagaaggat ctaatgtaac ataaggatgt
     4741 tcccaatctc ctgcaactcc taggcgtttg aaatctgctc gttgcttatc tacttgggaa
     4801 agtgcatatt cacgacattt ttccaagtat tctgccatgg acatttcttt acgtttcacg
     4861 cctttattag ccaatacttg ttcaatcggc aatccatgtg tatcccatcc tggaacatat
     4921 ggtgcacgat agccagacat tgattttgaa cgaatgataa tgtctttact gattttattc
     4981 aatgaatgtc ctaaatggat attaccattg gcatatggag gtccatcatg aagaacaaag
     5041 ctaggttttc cttcgttcaa ctcttgtctt ttttggtaga ttcgattttc ttcccattct
     5101 ttttgccact cgccttctcg gtttggcaaa ttaccacgca tagggaattt tgtttttcct
     5161 aattgcaagg tttctttcat tttcattcaa ccatacactc ctttatttgt tttccggaca
     5221 caaaaaagca tgttcagccg caagggacga acatgccgtg gtaccaccca aatttagttg
     5281 catctatcta gtgcaaactc tctgtgacca ataacgaggt cagccgctgc ttcttactct
     5341 aatttcggaa aagcaggtct caagtagagg atctttcacc atgcagtgtc ttaccgaact
     5401 tccactctca tcggctcgct ttaagaataa caaagtgctt ttctgttcta ctcgttttca
     5461 ttttcaccag gcacgatcga gatatcaata gcctgtgtat caaatgccgt cataggtgct
     5521 tctatgactc ctgtgtctga gtttacatca gtatcatttt cattgtcaag ctgttttgct
     5581 aaaacttctt tgatcacttc atggctgtca gtcacgtagc tggagaatgg ttgtaaaatt
     5641 tcatcccatt ccggactttt cacttgttct aattgagact caagcatcaa gctgatacgt
     5701 tgatggaata ctcgtgtttt tttcttaaga tcttcagttt ctgtcgcaag ctgtcttgct
     5761 ttttctgttg catctgtcaa gatttttctt gctttttctt ctgcatcagt tgttaattga
     5821 tgtgcacgtt tttcagcgtt cgcaactaac tcatctgctt tgttttgtgc agaagtaacg
     5881 atcacttcgg attccttgct tgcacttgtt tttactttat cagccgtatc ttgagctacg
     5941 atgattgatt ggttcaaagc atcttttaat tcattgaagt actcaagttt ttcttcagaa
     6001 tgtttgactg ctttttctaa ttctcgattt ctttgagtca attcctcata gtcacgaaca
     6061 actaaatcta agaaatcatc tacttcgtct tgattatatc cccgcatttt tgtcgggaat
     6121 gtcttatttt ggatatctaa tggagttaaa gccatgagta aaccaccttt ttattttatt
     6181 tacgtaatac accgaacagt accctgtact tttctttttt cgtcttcccc tcaagttctt
     6241 ggatctggac tctgccataa cctctgactg aaataatatc taatagttca agtacaaaat
     6301 caggtcttgt attttctgtc cagttcactt ttactttacc agcttcgatc agctgcttgg
     6361 accgctgtct ggaaatatta taaatctcag agatcacact gtctagtcgc atggaactaa
     6421 ctgttcctct ttcttgtgtc cagctatctt tcggcataat catttctgtg tatttgattg
     6481 gttccaagcg tacagcaacc tttccaatct ttgttacttg tgcagcaaca aaatgatcca
     6541 cctcatttgc aataaatact tgccagcgat caccgtccga gatgatatcg ccaaagtaag
     6601 agcgtttgat ccccgttccc actaaagttc caaggatttt cccatgactt aggctggcaa
     6661 atttgctagg ataatggatt tcatacaatg ctgtttcaaa atcttcctct acaggttcgt
     6721 aataatcggg gaaaatcaaa catctttttc tttctgcctg ttcatatccg ccataaaaca
     6781 tgaaacgcaa atcactgctt tgtcgaatca gtgtttctaa aatataggat tgtcttgggt
     6841 caagaaattc ggtcagatag ggtgcatatt gtccttgaac ctgttcaatc caatcgccta
     6901 ccgaatcgat aaatgtacgt tcatctgccc gaaagtgctg gtatacattt gcatccaaaa
     6961 ttttcccctc ctatctattc tgagaataaa gattttgttg cttcaatcaa taaactaaat
     7021 ggacagttat ccatataacg atctgtgcta aaccttgtac ggctaagttc aagatcagga
     7081 ttgcccctaa aattgtgaaa tccaagggtc caatcctcaa aggcagacga tcgaacaaac
     7141 tgatatacgg ttcgcagatc ttagctaata atcgtcctag ccctgattga tacccgcctg
     7201 gaaaccaaga gagcaatgca tataccatca agataatgct atagaaatag gctgcccgtt
     7261 gaagtaaatg aagtaatagt gcaatagtat aaatcgtagt tcctcctctt acagatcaaa
     7321 caaattcgca tctgcaaaag attgagcggt tgaactgtca atctccatcc cagtcggtgt
     7381 acaaagaaaa atctcatctc caacacgttt gatatctcca tctagtgcat aaactgttcc
     7441 tgttagaaaa tcaacgattc ttctcgcttg tttttcttct actaaatgaa aattcactaa
     7501 ggttgcttct ccagcaatga ttcgtttagc aatcgccata gcttcagaat aagctcttgg
     7561 ttccattaca gtgatttttc cagcaagatt tgctttaggt tgtgtagagg ttcttgaagc
     7621 tgttctttct ttcgctacag tttttgtagg tgattgatgt agttcaatta cttttttctt
     7681 ttcagccggc tgaatacttt catgcgttac aggacgtgaa gtcgccgttt tttgtttttc
     7741 atgagtattc gcagcagata cagaatgagt tgattgattc tgtgttgtat tttttttagt
     7801 tggctgtaca gccttttttg ttgtttcagg ataatcttgg tattcgtact cttcctcatc
     7861 agctaatccg aaaaaattag caattgctcc tctgtttaaa agcggcattt ttgttcctcc
     7921 tttattcgtc tttgaatagc gctgttccta cacggataaa tgttgcacct tcttcaatcg
     7981 caatttgaaa gtcttgactc atccccatac ttaattcact acatggtgca tgggcataac
     8041 cttttgcttg aacaagatca cgcaattctt tcagcgtacc aaacacttta tggatttgct
     8101 catcagaagc ttctttaggc gccatagtca tcaacccaac aaccttgatt tttgtgcagg
     8161 cagctaaaga ttccacaaac ggtaaaagct catctggtga aattccatgt tttgattcct
     8221 caccagaaac atttacttca ataaaacagg ataattcttt ctctaaccgc ttctggattt
     8281 cttctgctaa tcttaaactg tccaacgcat gaaagtaatc aatttcattt acaatcaatt
     8341 ttacctttct gcgctgtaaa ttaccaatta aatgccactt tatttcagga aaatcaaata
     8401 aatcttgttt cttttctaat aatttatcta ctcggttttc tgcacaattc ataacgccag
     8461 ctctaaccaa ttcttcagct tgagcggatt caacagattt tgttacagct actaatgtaa
     8521 cttctttcgg atcacgatga acaagagcac acgaatcctg aatttcttgc ttgattttct
     8581 gcaagttgtc agcaatcatg tgctcttcct ctcctttatt ttttacgacg gaaaaacggt
     8641 ggcgtattta attcatcatc gccatgagat tttgtttctt cgcggttgaa tgtatcaaat
     8701 tctttttttt cgatattgtc aaaattggaa tcatcaactc taggacgaac attttgttca
     8761 cgtctgatat cccaatcgcc aaaactgttt tcttcttctg aagaagttgg tttagcttga
     8821 tccatatcta atacagtctt ttgagcaggt gattgcattt gtgcttgtct agctggtcgg
     8881 ctagacttgc gttccttttt tgattcatcg atacctgttg cgatgaccgt cacacggatt
     8941 tcatcgccca tttcttcatt gatagatgta cctagaatga tatttacatc gccagttgct
     9001 gcatttgcta cgatatctga agcatcttgt gcttcgaaca atgtcatatc caaaccgcct
     9061 gtgatgttaa gaagtacttg ttcagctcca tcaatagatg tttcaagcaa tggagaggag
     9121 attgcttttt tcgttgcttc tactacgcga tcttctccac tagccacacc gatccccatc
     9181 aatgctgtac cttggttttc catcactgtt ttcacgtcag caaagtcaag gttaacgtaa
     9241 cctggcgctg tgatcaaatc agagatacct tgtacacctt gtcgtaaaac attatctgct
     9301 tcacggaaag cttcaagcat cggtgtcttt ttatctacaa cttctaataa acggttattt
     9361 gagataatta ataatgtatc cacattttct ttcaaacgag cgataccttc tgcagcaaaa
     9421 cggccacgtt tcggtccttc aaaagtgaat ggacgtgtca cgacaccaac agtcaatgcg
     9481 cccaattctt tcgcgatacc tgcaacgatt ggtgcagcac ctgtaccggt tcctccaccc
     9541 attcctgcag tgatgaaaat catatctgca ccgtctaaag cttcgcgtaa ggattgttca
     9601 ctttcttctg ccgctttttg tccaacttct ggctgagaac cagcacctaa accacgagta
     9661 tatttggggc ctaattggat aactgtttca gcttttgaat tttttaatgc ttgcacgtct
     9721 gtgttggctg taatgaattc aacaccttta acgttttctt caatcattcg gtttacagca
     9781 tttccaccgc caccaccgac tccgatgact ttgattactg cgccgtcatt gatattatta
     9841 tcaattgaga attccatatc ttattcctcc caggatgaat taatcaaaaa tatttgtaaa
     9901 gaaacccttg attttatttt ttacattttc tccggactct tttggttccg gctcatcgta
     9961 gattgtttct tcaggtgctt catagctctc atatgtgttt gttgtttctt gttcaactac
    10021 catttgacga gctgctgagg tttcacccgt aacagcaatt tttgccaatt gatacacttc
    10081 actcaagttt gctgaataat ccacgatact tattacattt gtaaataccg ggtttctcag
    10141 ccccatgtga tttggaacat acaattttac atttacgccg aagatttctt gtgccaagtc
    10201 cacaacacca ggaagactag cagctccgcc tgtcaatacg acgccacctg gcaactctaa
    10261 tgcttcgatt tggtccaatg catcttttgc tttgttgaaa atctgttcca ttcgagctga
    10321 aataatttct gacaagtagc gttcatcgac tttcactggt tctgactgac caatcacatc
    10381 tactggaaat tcttcatctg gtgatgtccg ttctgggtat gcatccccgt agtttatttt
    10441 caacgcttct gcattgttaa atgacgtatt taatacaata gagatatctt tagtgacaaa
    10501 ttcgccacct tcttggtcaa gattggtaaa cttcaattgc ttatcatgca taacagccgt
    10561 tgttgtttgg ccgccaccca tatcaatgac gatcgtacca aaatcttttt ctccgtcaga
    10621 aagaattgat tcagtcagcg ctaaaggagt aatcaccatt tcgttgacga ttaatccggc
    10681 attttccacg catttacgta tgttatggat gatcgtttta ggtcctgtaa acaacagacc
    10741 gtacatttct aatcttacgc cgatcattcc acgaggatct ttgattccct cgaacccatc
    10801 gacagtaaaa tcttgtggca atattgacac gatttgacgt tcaggaggta ttgaacgaac
    10861 aagggcagct gaagccacat ttctgacatc ttcatcggtg atttcctttg aatctccgtt
    10921 aactgcaatc attccctggc aattttctac ttcaagcatg tttgctggca aaccgacact
    10981 tacgcctttg atctggatac ctgctttttc ttctgcttgt cgtactgctc gttgtatcgc
    11041 ttggaccgtt ttatcgatat caacgataat gcctcggtta atcccttctg attttgcgtt
    11101 tcctactcca ataatattca tttggctgtc gatatattca gccacgacaa ctttaacaga
    11161 tgtcgttcca atatcaaggc ctacatacat tcctgttttt gccatgaatg ggattccctc
    11221 ctacttacta ttccatatac aagcgaacaa ctagcgtccg ttttaaaata tacttattaa
    11281 tagatatatt cactgcttcc aattctacca taaattactg gtttttagaa agtacattct
    11341 tactttttaa agttgtttta tcaattttca tttaaacttg acggagttgt acttggatta
    11401 tcttctattt gacttgtttc acttgaatca tttgaatcag atgatccgtt tgtgctagac
    11461 gatccaggtg tttgtgcttt ttgcgtttct gcctcttcac ttgattcgtt tgtttgatcc
    11521 gcattttctt cactttcagt tgtttcttcc gattggcccg attcttccgt ttcttgagtg
    11581 ctaccgtaag gatgcgagaa gataccaact tccatgtcga tcacaccttt ttcatccata
    11641 tctttggcta cttgcgaata atatttcatt tggttcgcaa gattattgat atttacaata
    11701 acttgattgc cgtcattcat attcaattgg atcagatttt tattgcttgc tcttggcgtg
    11761 tacttgattt cagaaattgc tttttgtaat tctgatgata actgattgta ttgctttgtt
    11821 aattctttga tttttgcaga gtctttgaac tcttccaaga tagggagatt ttttgtagga
    11881 tttgctactt tttcatctaa tactgttcca ttttcaatca ctgggtagta ttgattgttt
    11941 tttgcgatca aagcaatctc tttatattct gttacatcca aatcgaattc attcatccct
    12001 ttgaaatgta tatttgctgt ttcgattctt ggctgtgctt ttttaaggcg atcgatataa
    12061 gtagatcgat gaaaatactg tccccaaaca ttttctccta atgacaattt tgtatcagag
    12121 atgatttctt ggctgttgac tgtcttattg cctgatactg caactgcttg taatttgcta
    12181 taaggagaaa cataatagat caaaaaaatc agcggtatac ccaatacagt gatgatcaaa
    12241 gctaaccgac gaaatagtac tttgtgccgt tgacttttta aattgggcag ccggttcaaa
    12301 aaagaaccgt tatatggtcc accgtttttc ttttgcttga tctcttcaaa aaattcgatt
    12361 tttttctcgg actggctttc ttcttgctgt tcctcttgaa ccgttttttc ttcttcagaa
    12421 tctgaaaggg gtgtatctaa tgttatgtct tctttttttg gctcttggtt tttcatctct
    12481 tgttctgcca gatattttcg gttagccttt tgccaaggag tcaaattgtt ttcatcagaa
    12541 gacggatgag acaatttttt tggttctttt ttcttactca ctaagaccac cgcctttatt
    12601 ttttgattat ttgttttact aagtcataaa gacgttttga agcatcaggg attccttgtt
    12661 ctttagaagc tcggcacatt tgcttttgca attcttcatc acccatgatt tcgttgactg
    12721 tctgacttag tgattcacct gttagttcat tatctgcaat catttttgct gctcctgcgt
    12781 gaacaagact cattgcattt ttagtctgat gatcattcgt aacatatggg ctgggaacca
    12841 acactgccgg taaccccaga gctgtgaatt ctgcgatcga agttgcacca gcacgcccca
    12901 ccagcagatc actggaagcc atgacctctg ccatattttt gatataaggc tggacactaa
    12961 tattttgtat ttcaggattt attttattta ggatctcttc ataatatcta tctcctgaag
    13021 catacaaaac ttggtaagaa gaaggaatat attcacttaa aaaaactgat actgcttgat
    13081 tgattttaag tgctccttga ctaccgccaa aaatcaagac tgttttcttt tctggatcca
    13141 aatcaaatgt tttcaaaata tctgattttt tagtatctgc tacttcttgt gctcgaggat
    13201 tacctacaag aactgctttg ttttcaggaa aaaaggaagc cgcatcttta aatgaaagag
    13261 cgatccgatc cacatatcgg cttaaaaatt tattggtaac acctggtaca ctgttttgtt
    13321 catggataac tgttggaata cccattttgg ctgcagcgta cactaccgct ccggaaacat
    13381 atcctccagt accgataacg atatctggtt gaaaatcact gaggattttt tttgcttgac
    13441 ggatactctt aagaaacaat cggaccgtct tgatgttatg catggaaatt tttcgtttaa
    13501 atccttgaat ctccaaggta tggaaaggca ttcctgtatc agggacgatt ttgttttcta
    13561 gacctcgttt tgctcccaca tacatgaatt cagcattcgg ttcttgagtc tttacataat
    13621 tgacaaaggc aagagctgga taaatatgtc cccctgttcc tccgcctgta accaaaattt
    13681 tcatttttta tcgctcgctt ctactttttc tattttaatc ggttcacagc ttccatgaat
    13741 ctgtttcctc tagtttcaaa atttgggtat tgatcccagc tggcattcgc tggtgataat
    13801 aaaactacat caccatctgt actgttttct aatgctaaag gaactgcttg ttctacagaa
    13861 tcggctgtta ctattgtctc gatgcctgct ttttttccag catccatcaa cttttctttt
    13921 gtttggccaa atacgaccat tgctttgatt ccttttaatg aaggaattaa ttcatcaaat
    13981 gtatttcctc tgtctaatcc gccagccaat aacacgacct tagacgtttc aaatccactt
    14041 agagccattt tagtcgctaa aatattggtt gcttttgaat cattataaaa ttttctgcct
    14101 tggatttctc ctacatattg cgtacgatgt ggtacgccat ggaaaaaatg caacgtttct
    14161 cgtatagctt catttgatat accatacaat tttgctacag aaatagcagc tagtgcattt
    14221 tctacattat gcttacctgg tacgccaagt tcagatattt ccattatttt ttctttttta
    14281 tagtatattg agtaatcgtc agcatacaca ccgtcttcca acacttcttt tgtcgagaaa
    14341 ggcagtacgc gtgcttttgt tctttgcgct aattcttgta gttctgactg attccagtta
    14401 aggatcagat aatctttttc tgtcatgttt ttttgaaggt tccatttagc tttgacatat
    14461 tcttttcttg taccatgata atcaatatgt gcttcataga tattggtaat caccgctata
    14521 tgagggcgga aatctgtgat tcccatgagt tggaagctag acaattccat cacaatttta
    14581 tcttctgatt ttgcttcttg cgctacacca cttgctggat acccaatatt acccgctaaa
    14641 cgtgcaattc cttgatctgc tcctgcattc agtagtagtc ctgtcatcgt tgttgtcgtt
    14701 gtttttccgt tcgttcctgt aatcccaata attgggcatt ccgctacttc gtaagctagc
    14761 tcaacttccg taattaccgg aatacccatt tcttgggctt ttgctactaa aggatgactg
    14821 tagggaatcc ctggattttt gaccatcaat gaaaattctt catccaacaa ttcgattgga
    14881 tgactacctg ttattacttt tatccctaaa gaaagcagct cttgtgcttc cggattttcg
    14941 tcaaaaggtt ttccatcatt aacggtgaca agtgcgccca attcatgtaa taatttggcc
    15001 gcacttactc cgctttttgc gagtcctaag acaagcactt tcttattttc atatgttgat
    15061 atttttttca tttcccgtca tctcctacca aacgatccat aatgtgatca acgaagtcac
    15121 aatgctgatc aaccaaaaaa ctgtatcaat cttccactcc gaccatccgc acatttcaaa
    15181 gtgatgatga attggagaca ttttaaaaat tcgttttcct gtaagcttaa aggaagtaac
    15241 ttgaagcatc acacttgctg tttccataac atagatcaaa ccaatcaaaa gcaatgtcca
    15301 ttcttggttt agcattatag aaattgccgc taaaagtccg cccaacgcta atgaaccgac
    15361 atctcccata aaaatcttgg caggttttct gttgtagacg aagaaaccca gtaaacctcc
    15421 taaaacactt aagcaaatga ttaatacatc atactgatgc tgatgccaag caattagacc
    15481 gtaagctgca aaagagatac ttcctaagcc agcaactaat ccatcgatgc catctgtcag
    15541 atttactgca tttgaaaatc caacaagcca gaagagagcg aaaataccat aaatgattcc
    15601 taaaggtaga tctagtccga agaaattcaa tacacctgga taaccttcag aacggtaaac
    15661 aagataaaag atgattcccc ctagaatctg tccaatcaat ttctgcttcg agttcagtcc
    15721 catgtttctt ttcttgaata tttttataaa atcatcaaga aagccgatta ctccgtataa
    15781 cgctaataca aaaagcaaga tgaaaagagt aggagttagc tgtttttgcc aagctccaac
    15841 ccaaattcct gtcaaaatag agccgatcag aaacactaag ccgcccattg taggagttcc
    15901 ggctttgcta ttgtgccact ttggtccctc ctcacgaatc gcctgacctt gctttttcat
    15961 ttgaaaataa ccaataaata gcggcattgc ggaaattgtc attgcacaac tgctgacaat
    16021 gggaatcaat gcttgtgtcc attccatgct cttttctctc ctatttctct tcttattctt
    16081 ctaaagtaaa actaagtttg tcttcagtga ttttttcata tggagcaagt tcttgtttga
    16141 cacaataacc atctccatca aaactaactt caataccgag gattttcccc agcttcatga
    16201 tatctgcttt tgaccatcct gttacgtcag gcatcagctt atcaccacca gtatatagga
    16261 tcagtttttc tccagagatc aactgatcac cgttggcagt agattgtttt tgtacttttt
    16321 taccattacc gatcactaca ggttgcagtc cacttttttg agcatcagct gctgcaacgt
    16381 ccgcttcaag attacgataa tcggctacgg atactttttc tgtttttgtt tctgtatctg
    16441 aatcttcttc agtttcttta aaatccattg cccgtttcat caatggattt gcgattttag
    16501 ccaatgccat tcgatcatat gtcttagggt gtttcattgt tagatacaag acatagtctg
    16561 gatcttcaga aggaaccatc tccacaatcg aatacaggta ggctgtatct cctgtttgat
    16621 atccccctgt atcagaagcg atttgggctg ttccgttttt tgccgataca ttgtatccag
    16681 gtacactata tacaccataa gcactaccgt agttttcgct ttcgacaaca tctctcatat
    16741 attcacggac tttttctgtt gtttcttttg aaaaaggttg ccctagaact tctgtttgag
    16801 ttgttcgctc ttctccagtt tggggatcca ccactttgct gatatatcga ggttgaatca
    16861 ttgtaccgtt attcgcaata gaagtgaaag ctttcatcat ttggaaattc gtcacaccta
    16921 ccgcttgtcc ataagcactc attgctcggt cgacaatatt taatgtaggt aaagcaccgt
    16981 ttacttcatc atctaatcca gaatgagtac tttgtccaaa tcccagtttt tgtaaatagt
    17041 tataccatcg tccgcctaat cgctgttcca gtatgaccat cccgacgtta cttgaccatg
    17101 aaagagcttg acgcatggtc agcacacctt tttcaccaaa gtcatggtca ttgatcgtag
    17161 catcttctac ttggattttt ccagattgga aggtttcatt ctcgttgaat tctccttctt
    17221 cgatagcagc tgcagtagtg aaaactttca tggttgatcc tggttcataa ctatcttgta
    17281 ccaagaaatt acgccagata gcatcttttc cagtcaatcc ttccattgtt tcaggattga
    17341 aagtaggtcg ttgtcccatt gccaagattt ctccggtttt tgctttcatc aggactgctg
    17401 tcaattcttc tggctgatat tcttcattta cttgatccat cagtgtttct aaataacttt
    17461 gtagtctgct gtctaaagtc gtgtagatat cttgtccgtc gacagctttt tcttcttctg
    17521 ccactgtacc tggcagagga ttttgaaaat tatctttttg ataaataatc ttaccatctt
    17581 taccgctcaa gatatcattg taagcagact ccaaacctag tttaccaact aagccattct
    17641 catctttgtc tggaactgcg tatccaataa aatgagaaga aaagacgcca tttggataca
    17701 tccgagcctg atggtcgaca aaataaagac cagcgacacc ttcttttttc atgtccgctt
    17761 cgatgttttg cttggtttct tgggagatat ttttcccata gctaccaaat tcaacttgat
    17821 acagctcttt ttttgcgccg tcctctaaaa tattcagtgc atcttttttg tctatagaaa
    17881 gattcttatg caggatttct gctattttat caaaattctt ttcttcaaca tacagttttt
    17941 tatccccaga ggtatatgtt tttgagagta tcgctttaat tgaataagag gaggcatctt
    18001 ctgcgagcgc cacaccgtta cgatcataaa tcgttcctct ttttgctttt actacttcac
    18061 ttccttggta caactgtttt gttttttcag ctaatgaggt accagcaaca tgacctccaa
    18121 taacgatata cgagaaacgg acggcaaata aaaagaacaa tccaatactc gtagcaaata
    18181 aaatgatacc tacttttttg cgattgttca tcggatttag atttttcttt tccataaacc
    18241 gccgaatttt atttttaaga ctcatttact tcactttcct taaattgtcg tcattaatag
    18301 acaatccttt ttcttctgcg atttgtttaa tccgttcagt acgagaaagt tcgttttttt
    18361 cttgttctaa actcgttttt tcttgctttt tatcattgat ttctgtttgt attgacgtga
    18421 tttctttttc agctccgctg atatttgtgc gcagcataac tgttaaaaca cataaagcaa
    18481 caatagcaat cgccacgctt gccacagcaa ttttttccaa agcagaaatc tgctttaatc
    18541 gttttgccgg tgattgtgga agaatgattt cttgattcga agaagaatca ggcatttgtt
    18601 gttccggctg atccaccaat tcaggtaaat catattcgta atcttgcatt ttctttaatt
    18661 ctgccatcat tctactcctc tttcattctt attttttctg caatacgtaa ctttgcactg
    18721 cgtgatcgat tgttttctgt tagctcttcc tctgaaggaa ggattggttt acgagtaatg
    18781 actttcagtt ctggttggaa ttcgtcaggt atgacaggaa gtcccggtgg tagatcttgt
    18841 accgtactat attctttaaa catacttttg acaattctgt cttccaatga atggaatgtg
    18901 attacgctga ttcttccatt gactttcaat aaccggattg cctgttctaa agaggcctct
    18961 tcggcaccga gttcgtcatt tactgcaatc cgcacagctt gaaaaattct ttttgcagga
    19021 tgcccgcctt ttcttcttgc gggagctggg atgacttgtt taatgatttc gacaagctca
    19081 ccagttgttt ggattggttt tgtttttcgt actcgttcga tttcacgagc aatttgtttg
    19141 gaaaatttct cctcgccata gcggaagaaa atctttacta attcatggta ggaatattca
    19201 ttaattacgt gataagcgga taaaggagct gactgatcca tgcgcatatc caacggtgca
    19261 tcttgatgat aactaaatcc acgttctgct tcatctagct gaggagagga aacccctaga
    19321 tcatacaaaa tgccgtctac cttatctaca tgctcactca agcgttcttc taattctctg
    19381 aagttcgctt tgataaatgt gaccatacct tgttcgacat aagaagctaa acgcagcttg
    19441 gcgtgatcga ttgctttttg atcttggtca aacgcataca gatgcccttt agaacttagt
    19501 tggcttaata aatattcact atgtccggca ccgccgagtg tacagtctac ataaacgcca
    19561 tccggtttga tgttcaatcc atctaccgtt tctttcaaca tgactgtata gtgttgaaat
    19621 gtttcagtca ttagtcagcc tcttcctatt cttatagtcc gaaatcaatc atactttcag
    19681 caatttcgtc aaagttctct tctgctgctg cagaaaaatc ttgccatcgt gtctcatccc
    19741 aaatttcaat tcggttagat acaccgatga ctacgcaacc ttttgtcaaa gctgcataat
    19801 ttcttaacgt gctgggtata ttgattcgtc cttgtttatc aagttcgcat tcagtggcag
    19861 ccgaatagaa gaatcgcaca aaagtacgtg cgtctttttt agccaacggc atttcgttca
    19921 gtttagcttc cagattttcc cattcgctca tcggatagcc gaaaagacag ccatccaatc
    19981 cgcgtgtcag aacaaacttt tcgccaagtt cttcacgaag ttttgagggc acgattaaac
    20041 gtccttttgc atcaatatta tgttggaatt cacccatcaa cattgaaagc cccccattcc
    20101 tccactaact aaaattagtc taccacaatc ccccactttc taccaccttt tctgggaaat
    20161 ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc aaaaagcctt
    20221 ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat gttcacaatg
    20281 atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt catcttgaaa
    20341 taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat acaaattccc
    20401 attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt atttctagac
    20461 aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt tattttaaaa
    20521 cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac gattaccggg
    20581 taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa ctcctttttt
    20641 atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta tttaaattta
    20701 tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat
    20761 aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga
    20821 gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc
    20881 taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac
    20941 ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac
    21001 aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt
    21061 tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat
    21121 tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga
    21181 tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa
    21241 cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc
    21301 cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag
    21361 agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca
    21421 agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat
    21481 gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat
    21541 cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga ttattttaac
    21601 tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag
    21661 ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg taactaccta
    21721 ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg aaaaaagatt
    21781 gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa aaaaacaaac
    21841 aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt taacgattgg
    21901 atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag ctatcagctg
    21961 ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt ttcaatagtt
    22021 cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata cgtgcaattt
    22081 cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt ccatcaatca
    22141 ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc agatgagtga
    22201 tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg gcctgtgcaa
    22261 ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca ccttgctcag
    22321 aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca gaagcgactt
    22381 tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg atttggtcaa
    22441 atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca aactcagtgt
    22501 tttccatata cagacttttc aattcatgca gaatggattt ttccaattct tttgcgattt
    22561 ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct ttttgctcta
    22621 gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct agctcttcgg
    22681 tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta cgtttcaact
    22741 gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct aactccaata
    22801 aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa gcactttgga
    22861 tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc agttctgaaa
    22921 cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct ccgtaacctg
    22981 cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct tcttttaatt
    23041 tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg aaatggagca
    23101 tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc cttttttcca
    23161 gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg aaagctacat
    23221 ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc tgatgttcgt
    23281 tttgtccttg gatatctact aaataactgc cgattcttct cagattagct aatgtgataa
    23341 tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt cgaacaatca
    23401 gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat ccttcttgtt
    23461 ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt atataatcgc
    23521 ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat ttacccgcac
    23581 cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat tccggaataa
    23641 tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa cttaaaggta
    23701 atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa tcatcaaaac
    23761 attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat caatcaaatt
    23821 tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt ccatttggtc
    23881 aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat cttcgtttgt
    23941 ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga tcagtttcag
    24001 ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc ttttcaggat
    24061 ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag taatcaaacg
    24121 atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct taatcaatga
    24181 tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg attttcacag
    24241 ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct gccatattaa
    24301 tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag aactcgatat
    24361 ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat ccaccttgtg
    24421 ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca cgaacaattc
    24481 cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa gcaatcactt
    24541 ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt gaaataaaag
    24601 aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt tttgcataac
    24661 gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc caagctagct
    24721 gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa gctgcatcag
    24781 taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca gatagatcaa
    24841 atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga agttttttac
    24901 ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca aaccgctcgt
    24961 ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct cttgtttcaa
    25021 ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt tccctcattt
    25081 ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag aaaaaacgtc
    25141 ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca acgcctcacg
    25201 tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt catctttacc
    25261 agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat ctcttatctg
    25321 aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt cttcaggttg
    25381 cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg tctttctttc
    25441 atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga tatctgcagc
    25501 ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa gcagcagaag
    25561 cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg taagcaaacc
    25621 atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag gttttcctct
    25681 acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat acgtatgcac
    25741 catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat caaaggaagc
    25801 aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca ctgaataact
    25861 catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca agatttcttt
    25921 ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag ctttcctcgt
    25981 ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag gtctcttctg
    26041 ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta aacgaatcta
    26101 gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt tctaattctt
    26161 gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc tatctcttgt
    26221 acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc ttgatgaatc
    26281 tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc tcttcccatg
    26341 attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg ctgctgctgt
    26401 tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc agtttcttgg
    26461 cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct taatgcgatc
    26521 gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg ctgtttcaac
    26581 tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa tacataggaa
    26641 cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa cgcctgctgc
    26701 aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg aacagctaat
    26761 tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc gatcgtggta
    26821 tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat cgcccgagca
    26881 acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg accgatgatc
    26941 attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac attgtccgct
    27001 gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg atatcttctt
    27061 ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag gaccgcgatt
    27121 cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc cttcatcagc
    27181 ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg ctccatgtgc
    27241 tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc aacgacaagg
    27301 actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt gaacataata
    27361 gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt ggcacgtaaa
    27421 cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc ggcatcaaat
    27481 tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc catctattca
    27541 cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt gatagtcatt
    27601 ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac ttcatcaaat
    27661 ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc tatcactaca
    27721 gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc tacgactaaa
    27781 atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc aacagtaaca
    27841 gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt gcttcgtccg
    27901 ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat tttcatgatg
    27961 ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc taaattcatc
    28021 ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt ttcttcatct
    28081 atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc ttgattgtat
    28141 ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg ctctaccaat
    28201 gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat ttggctggct
    28261 ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc tttcaatttt
    28321 tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc gttaatcaat
    28381 tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata gaattttccc
    28441 gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc cccagcttct
    28501 aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg gtcatcacta
    28561 aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac atctgtcaca
    28621 taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga gatccgattg
    28681 atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc tttttttgca
    28741 cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc atcttcgttg
    28801 atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt tgttaggtct
    28861 gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc gtgtcttgat
    28921 aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg ttggttgttc
    28981 taatttttca ggaacaacag ctactacatg gatattgact tcagataaat cgatatccgt
    29041 catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt taggaactga
    29101 aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct cgtttgctaa
    29161 gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc tggcaaaagt
    29221 tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa ttacttcgat
    29281 gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa tcaaattttt
    29341 ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc cgaattctct
    29401 atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg caaactgcta
    29461 tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc ttctatagtg
    29521 tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt ttacgcttac
    29581 ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt caacacacga
    29641 tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat tcctggtaag
    29701 tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc tgcacgagaa
    29761 gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga atgtccaaat
    29821 gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa ttggacacca
    29881 gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac tatttgataa
    29941 atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat atcggaaacg
    30001 tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc gatgattttt
    30061 ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat cgtatcaaaa
    30121 gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc gacttcaatc
    30181 tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc cgcaatatga
    30241 catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag ttcttcgatc
    30301 atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga atattctaaa
    30361 aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg tactaagttt
    30421 gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg agcctgcgct
    30481 gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa agtaataaca
    30541 gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg actggtaacc
    30601 aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac acgtagcatc
    30661 ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact tttcttcgta
    30721 cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa ataaagccaa
    30781 aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa aacttatgat
    30841 cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa aatagatgga
    30901 ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg tccaatactt
    30961 gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc tttactggtg
    31021 atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa ccaactcata
    31081 atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg gacaaataat
    31141 tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa cctcctctta
    31201 gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg tttcactaca
    31261 tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct gatcgcacga
    31321 agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt cgtttgaatg
    31381 gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata atccacttct
    31441 tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc aaaaccatta
    31501 tcacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc atcaattacc
    31561 aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt tgtttatgaa
    31621 cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta tggattataa
    31681 aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga taacgattgg
    31741 agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg cagctaaacc
    31801 aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag aaagggctaa
    31861 gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag gcaatttatc
    31921 ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta agaatttgat
    31981 tgatacccat ctttctacaa gggcagctaa tacgaacatc cctaaaattg aagcaccttt
    32041 tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac cagctttgta
    32101 gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac ctaagaagaa
    32161 gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg cacctagcat
    32221 tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac ccatcatacc
    32281 tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac gttcttcttc
    32341 cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat taaagaattc
    32401 caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt ttttgattgc
    32461 tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc atgaaccttg
    32521 aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt tgattttttc
    32581 ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc gcctaaaggg
    32641 tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga aaggttcaag
    32701 taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa ttgagaaatt
    32761 gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc catcatgttg
    32821 attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat accatctgtt
    32881 aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat aaatagcaac
    32941 gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat gtgccacatt
    33001 tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac gattggaaca
    33061 gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg aacggcaatc
    33121 gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat aattgctgat
    33181 gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc ccaacctaaa
    33241 gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc ggtaactaaa
    33301 ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag aataccttcc
    33361 ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga catgataagc
    33421 ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt gcttttttca
    33481 aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg aatttcacac
    33541 ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac aatactttac
    33601 ttacaacgac tttaccgacc gaatgcgcca ttgacccaac atttaattct ttgatgtcta
    33661 cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc aatgctttgg
    33721 tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga ataacatttg
    33781 cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca tcctttgata
    33841 ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa gctgtcgcaa
    33901 cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt tttccgtctc
    33961 cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact gctgctttcg
    34021 gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt gctgtttcaa
    34081 tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg taggcttcaa
    34141 tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc tcttcgaata
    34201 atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat aacacttcgt
    34261 cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca tctggacctt
    34321 cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc attgaaccgg
    34381 attgtaagat tccttcagca aattgtccat gacttgctag gataatccct accatattta
    34441 tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc taaccattac
    34501 aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg attttcgcta
    34561 gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct aactccactt
    34621 cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa acaaaattgg
    34681 taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac ttcaactgta
    34741 caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc atcacttttg
    34801 ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt attacatttg
    34861 ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta tcttgagcga
    34921 ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa actgttgcga
    34981 cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc tgcaaaactc
    35041 cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac cacctcctag
    35101 cacacactac tataagcaaa aagcgtgcca acttttttac gatacactaa ttgaatttaa
    35161 aggattctta accaatacac aaaaacgata cactataaaa gtgtatcgtt tttgtgtatt
    35221 catggcacag tgtattcttt tttatctaac tgattatgga ctagacgtag cagataatac
    35281 atttcttcta tcggcacttt tacttttatc tgatcttcca acagaacaac tgaggctgcc
    35341 aattggctat aaagaggatc attcgttgta tttgtcaatt cgttttcttc cacagtcaat
    35401 ggttcattcc gaataatacg ttcgatcatt cctgctgtgt gcatcactag gttgattttg
    35461 aatccatatt cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga aaattgccac
    35521 attggatcga ttaacttact accattaata aagatgaagt tatcagagat aaactcaaca
    35581 cacgtatttt tagctgactc ttcatctagg gagatgaatt cagtttcctc caattctgat
    35641 tccatcagca actgatccaa aatgacttcg atattttgat caataaaacg ttctaaagga
    35701 ataaatgggg cttggatatt caaatcagta atcccagtag tagctatgat ctgatattct
    35761 ttttggataa ctggcagttc ttctttcact tccacaatcg acaaagctag aacttctaat
    35821 tctgtttctt gacgcttgtt taccgctcgt tcgatcaatt ctttgattct ttgtgctgta
    35881 ccttcgccag atgcgcagat agccaaaaca gcttttttct tatgaatagg ttttgcttcc
    35941 tgaacagatt ctacagaaga tatttctgcg taacctcgaa aatttttcaa tgattcatgc
    36001 aacatatcta gatctgtccc taatactgag acctttcgta cagtttccaa aactagagaa
    36061 gttgttacca tatcaacagt acgtactgga attccagtgt ctctttgaat ttgcgaatta
    36121 aacgacgcaa gggaccccat atctactaag agtatgaccc cgcttccttc atctacttct
    36181 tgtacattgc gttcaattcg gtccaatgct gttttcggat ccatatccaa aggcatgtct
    36241 actgctcgta cgttctctgc atctaacaac tgttggacaa cttggaccat actgcttgct
    36301 gtgctgtttc cgtgagctgc aacgactaca ccgattctgc cgcttgcttg atttgcccgt
    36361 aacgagacga gtagaacggt caagtaatag tcttcacttt ctggaatctc cacttgaaaa
    36421 tacgtttcga tataactacg cacttctttc gcaacagcat attcatctgg aaaatcaaaa
    36481 gccatctcac ggatgttatc attcgtatgc cgttcttctc ctaagttgat ttttttcaaa
    36541 aaagaactga tatgcaagct catagcataa ataaagtttt gctgaaactc atatggtaaa
    36601 gatcttttga ccattacata aatctgattg gtcacttcta tgattttcgg atctacaaat
    36661 tctgctaatt tgctgtctgc attaaaagaa aaaccatggt ctttatagaa agacttcaga
    36721 tggatgttga tatctgtaga aatgaaatga ttgattgctt cttgatccaa accatccgac
    36781 ttcagtaaag ctgctttatc tccgatgata tcatatagat tataaggtaa ctcataggag
    36841 tccgattgga ttttcatgat cgtatcattt ggtgctacag ttatctttgg ctccaatatt
    36901 ttagaaagct ctgccattgc actgcggttg cttgctaatt gaatcaatcc gctacgaatc
    36961 ccttctgtca aatcatcgat tgtgatcgag atttcgggtg attgcatatg atttagaaat
    37021 cctcgtgcac atactaactg gatatttgac ttcaattgtc ctatatttcc ataggtcaca
    37081 ctgccgatca atgcttttgc tacatcttct gtcaaagaaa tctttcgctg cgtgcggttt
    37141 gcttcgtggg caatcatgat ttttactaaa tcgactttct catttgcagg acgtttttca
    37201 aaagccggaa gtttgatatt gattgggata cgccgaacaa aagtttctaa tagagaagaa
    37261 cctgggtctt ctgtggtcgc acctacgatc cgtacatttg cttcatggga tttcgttgtt
    37321 tccccaagac gactgtaagt tccatgatcc ataaagtaaa agatcatttc ttgaccttca
    37381 ggcggcaacc ggtgaatctc atccaaaaac agcataccgc catccgcttg atcgataatc
    37441 cccatttttt cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa taaatgactc
    37501 atcaaaagct cagggttatt ggcataatca gcacaattga aaacaatcag ttccgtttcc
    37561 tcatctatta catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa ataagtcttt
    37621 cctgatccgg taggtcctgt aatcaaacaa tttaatcctc ttggcggata caaaatcgca
    37681 gcttttgcct gttccactga atttttcatg ctgccgttag ctccaatgat ttttgcaaag
    37741 atatccttag tatctatttg gagtgtattt ttttcttcag agaccggcgt ttctttgtaa
    37801 ctgactacat gttttgtaac tggctttacg tgcgtaacga gtgtttgata tatgtaacgt
    37861 actggtcgtc cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt cagatcttta
    37921 ctgctatttg ttcgctgaat ccctaacact tcagcaattt cctgtgttgt tactccttga
    37981 tcatattctg cctttgttaa atgagcggtt ttttctttga catacatata aattcggtca
    38041 atgcgttttg ccaatggtat cgctcctttt ctatagacta ttgtacttag gcttcaaaac
    38101 ataatactat gtatttattt ctgtttcgtc aactatttac ctgattttgt tttgttgata
    38161 acacgcggat agatagaaat gttcaattta aaagaacgaa aaatcccgat acgattttta
    38221 taatggaaac aagaaaaaga acactgttta ccatataatt atttcactga tagcaaacaa
    38281 tatagcttcc atcaattgaa aacgatagtt atcaaaacat cctgattcca aattggcaaa
    38341 aaaatattaa gtgtaaaaag acaaagtaaa cttcgtagcc tccactacaa cttttatttt
    38401 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattc
//
LOCUS       DACCVU010000014        37318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCVU010000014 DACCVU010000000
VERSION     DACCVU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.014"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            61..738
                     /locus_tag="IVW29_000616"
     CDS             61..738
                     /locus_tag="IVW29_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838119.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            741..1868
                     /locus_tag="IVW29_000617"
     CDS             741..1868
                     /locus_tag="IVW29_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838120.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2068..2508)
                     /locus_tag="IVW29_000618"
     CDS             complement(2068..2508)
                     /locus_tag="IVW29_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7838121.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2519..4552)
                     /locus_tag="IVW29_000619"
     CDS             complement(2519..4552)
                     /locus_tag="IVW29_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7838122.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4554..6257)
                     /locus_tag="IVW29_000620"
     CDS             complement(4554..6257)
                     /locus_tag="IVW29_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7838123.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6351..7301)
                     /gene="manA"
                     /locus_tag="IVW29_000621"
     CDS             complement(6351..7301)
                     /gene="manA"
                     /locus_tag="IVW29_000621"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7838124.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7340..8365)
                     /locus_tag="IVW29_000622"
     CDS             complement(7340..8365)
                     /locus_tag="IVW29_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7838125.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8574..9443)
                     /locus_tag="IVW29_000623"
     CDS             complement(8574..9443)
                     /locus_tag="IVW29_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7838126.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9902..11794)
                     /locus_tag="IVW29_000624"
     CDS             complement(9902..11794)
                     /locus_tag="IVW29_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838127.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11791..13518)
                     /locus_tag="IVW29_000625"
     CDS             complement(11791..13518)
                     /locus_tag="IVW29_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838128.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13534..14184)
                     /locus_tag="IVW29_000626"
     CDS             complement(13534..14184)
                     /locus_tag="IVW29_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838129.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14388..14684
                     /locus_tag="IVW29_000627"
     CDS             14388..14684
                     /locus_tag="IVW29_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7838130.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14856..15476
                     /locus_tag="IVW29_000628"
     CDS             14856..15476
                     /locus_tag="IVW29_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7838131.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15568..16734)
                     /locus_tag="IVW29_000629"
     CDS             complement(15568..16734)
                     /locus_tag="IVW29_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7838132.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16746..17099)
                     /locus_tag="IVW29_000630"
     CDS             complement(16746..17099)
                     /locus_tag="IVW29_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7838133.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17227..18249
                     /locus_tag="IVW29_000631"
     CDS             17227..18249
                     /locus_tag="IVW29_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7838134.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18688..19122)
                     /locus_tag="IVW29_000632"
     CDS             complement(18688..19122)
                     /locus_tag="IVW29_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838135.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19232..21091)
                     /locus_tag="IVW29_000633"
     CDS             complement(19232..21091)
                     /locus_tag="IVW29_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7838136.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21209..22369)
                     /locus_tag="IVW29_000634"
     CDS             complement(21209..22369)
                     /locus_tag="IVW29_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7838137.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22676..24100)
                     /gene="araA"
                     /locus_tag="IVW29_000635"
     CDS             complement(22676..24100)
                     /gene="araA"
                     /locus_tag="IVW29_000635"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7838138.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24116..24814)
                     /locus_tag="IVW29_000636"
     CDS             complement(24116..24814)
                     /locus_tag="IVW29_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7838139.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24771..26432)
                     /locus_tag="IVW29_000637"
     CDS             complement(24771..26432)
                     /locus_tag="IVW29_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7838140.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26650..28050
                     /locus_tag="IVW29_000638"
     CDS             26650..28050
                     /locus_tag="IVW29_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7838141.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28302..29381)
                     /locus_tag="IVW29_000639"
     CDS             complement(28302..29381)
                     /locus_tag="IVW29_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7838142.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29467..30426)
                     /locus_tag="IVW29_000640"
     CDS             complement(29467..30426)
                     /locus_tag="IVW29_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7838143.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30438..31961)
                     /locus_tag="IVW29_000641"
     CDS             complement(30438..31961)
                     /locus_tag="IVW29_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7838144.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31971..32534)
                     /locus_tag="IVW29_000642"
     CDS             complement(31971..32534)
                     /locus_tag="IVW29_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7838145.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32540..33403)
                     /locus_tag="IVW29_000643"
     CDS             complement(32540..33403)
                     /locus_tag="IVW29_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838146.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33417..34313)
                     /locus_tag="IVW29_000644"
     CDS             complement(33417..34313)
                     /locus_tag="IVW29_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838147.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34488..35759)
                     /locus_tag="IVW29_000645"
     CDS             complement(34488..35759)
                     /locus_tag="IVW29_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7838148.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35749..37266)
                     /locus_tag="IVW29_000646"
     CDS             complement(35749..37266)
                     /locus_tag="IVW29_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7838149.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10810 a         7806 c         6455 g        12247 t
ORIGIN      
        1 atttaattta gatgttttcc attctattat gttattaaat tttatattgg aggcctatct
       61 atgacattac tgatcaaaaa catgaacatg aaacgtggta aaaaacgtgt gattcgacaa
      121 ttgaatcttt tagtagaatc aggagaagta gctgctttga ttgccccgaa cggatttggc
      181 aaaacaactt tgctgaatgg cattgcccaa ctgctgccaa caacttatga tcaattgtcc
      241 cttcacaaca tctctcctac tgcttataca gattatcgcc ggtctttctt ttttctagaa
      301 tcaacgaatc agttatccga ctatctatcg gctgttgatt atttgacttt gatcaaatac
      361 tattggcaga gcaaagtaga atgccaaact gttttccaac gattgaagat gactgagtac
      421 cttactttgc cgattggaaa aatgtctcta ggaatgaaac aacatgtctt gcttgctgct
      481 tacttgatca gtgattcatc tgtcatgtta ttcgatgaac cgttgaacgg attagaccca
      541 ggaagtattg atatcttgaa tagcctcatc agacaatggc agaaagaggg gaagacgatt
      601 cttctttctt cccacgctat cgcgaatatc caatcgattt gttctaaagc atgttttcta
      661 aaaaatggag aaatcgtcac agaatcctca aatatggaag agattttagc tatttatcgt
      721 aaattatatg aggtgtaact atgtctttac ttacatttga acgcaatctc ttactaaaaa
      781 acaaagtaaa ctttttattt cctctattgt tggtcgtgct ctttgcattc ccactttttt
      841 ttgatcataa acttgcttat acagaattcg atgaactgaa tcataactat gaagagatgc
      901 aacgtttgat tgaaacgttg aaagaagatg agaatgagaa agaatttgtg gaaagtttag
      961 agaaaagcaa caaactgatc gaagcaatct tacatgcaaa aaacacaggg aatgtccagc
     1021 aaatggtcga agcaacttat cattatgaaa aagatatact ggatcgactg atttctggtc
     1081 aaagacaagg aattccaatc attgaacaac aaaaacgtgt cgagttgctc cgttatatga
     1141 aagaacatca gatccaaaga tattctattt ttgacttgcc agcgcatctc cctttagcaa
     1201 attactacga aaatattttt tcaggaatga tttctagttt tttgatttta tgtataaccg
     1261 ccttgtttct ttcttccatc atctcttatg aaaaaagaaa gcaggtaatc tctctagtca
     1321 atctattacc ggatagtatg gtaaagaaac atagcatccg ttttaccatt tattatggtg
     1381 cagctatgct aagtctcgtg atgccatttt tgatcgtctc tatactagta ataatcaaaa
     1441 atggtttggg agacttccgt tatcctgtag gaacgatcat tggtcaggag atacgaatcc
     1501 ttccgatgta tgagtatctt ttccagagct ttctgttcct gttgttgtgg gtgctctttt
     1561 taagtacaat cagttttctt ctatctgctt tatttgaaca ttctctagtc aatcttttag
     1621 gtacacttct ttgtcttttc ttagcagagt atcgcttgtt ttcttcgatt ggatggatag
     1681 aatcaatcag tcattacttg ccaacttctt atgttgattt tcaaaatgtg atcataggag
     1741 gagatatttt ctcgccacta gcttcagaac aggttacttt catgaatggt atcttgacac
     1801 ttggtatatg gagtatcgtt ttattattca taggtatggg cactatctat ataaaaaaga
     1861 gctattaaaa cacaaaaata ataggggcgg tgacaaaaac cttgtcaccg tcccctattt
     1921 caaataaacg gttttctaga ataaatagtt ttcaaaagct tgcatccgca gtaataagaa
     1981 cctcccaaca aaaatagctc ctcttatttt tatcttggtt gaagttatta cttgatgcaa
     2041 ggcagctttt tttactgtga acattggtta tatatgtcca tgttcaaaaa tcatttggac
     2101 ttcttcaatg gttttcgcat cactgagcgc cattacttgg tcgatgtctg aacagaaaaa
     2161 ggctaaatct tggagcaccg ctaactgatg ttcttttttt aaagccacta caaataagag
     2221 tgtcgcgatt tttggttcct cttttgttcc aaagtccaca ccatcaggca cttggactaa
     2281 atagattcct tcttctacta cttcttcatc tttgtcagtc ccatgtggca aggctacgaa
     2341 attcccgata tagacactcg ctttttcttg tctttcgatc attgattgga tatatgccgg
     2401 tttgattccc ccttgttggg tcatccactg gccgatctcg gtcaacgctt ctttccaatt
     2461 ttgataggat tgatcgagca atattttttc tgctggtagc tgtatcatct tttcaccttc
     2521 atttctttat ttcttcaatc aggattttcg ctaacaactg ataaacgatt tctttatttc
     2581 ctgagcggaa gatctcggtg ttcaagtcat tcatgatgat tgctccactg atctttccta
     2641 aaagaatccc gtctttttct tggattggcg aaggcgccag cataactaac atacgggaca
     2701 aatcgactgg ctgtctatcc atcccttcaa tggtcaacgg atgtttcaaa ttgaaaatac
     2761 aaaagatcgg tttagaaatc aatgagctgg atgtatggaa taaccccata tttgtatgag
     2821 gaataccgat aggtgcttgt tcatatcgct taagcagccg gcgatgaacc acctcttcat
     2881 ccgttactag tccttcagga aaataaggaa ataagctgcg aatcgtttcg gaaatcgtct
     2941 caggattatc cagttcttgg ataaaaaaac gaccgagtaa ttgattgatt tgatccatga
     3001 aatgcattgt ttcctcataa gtatcttcta tcatccactc gttattgctg ttatacgtaa
     3061 ttttttgcct ttttttcttc tcaatatgcg aaaaagcttc ttttagctgc ttgacttcat
     3121 cttctagcag tagcggggag actaaaatat attttccacc gtatcctggt aaaatagatg
     3181 tagaaataac aacatcatat tcttcttgta aatcgatttt ttttaaatca gcaatccgat
     3241 agaaatgaat ttgttggatg aagggaaaat accttttcaa tcgtagttcc aacatgctag
     3301 tagaagctaa tccacttggc gaaaatcctg caatactgat attgctttct ttcggacttc
     3361 gttccaatga attcgcaaaa tgaaggacca tataagcgat ctcttcttcg gataattttt
     3421 tttcttgaaa aatccgctct actgctttac ggattgcatt cgcaacttcc tcgtattgaa
     3481 ccatgatttt ttctaaaatc ggattggtca gtgtctcttc ttgtaaaacg gctcttgaaa
     3541 acacaccaga taaatgagtc aacagcattt tgtacaaagt ttcatcttca taaaaatgga
     3601 cattcgtctc ttcacttact tcttgaatca gttgttttac taaataagca agctgtgtat
     3661 cgaacgtctc ttcaaagaaa ttctgatcaa atgagttcgt gaagtcattt aataatcctg
     3721 caaaaaagac gatctcattt actggataaa gttgttttgt ctcagaagcg atccgtgaaa
     3781 acaatcgttt agctgtctgt agtaattctt tgtttgccag actattatat gtctcatttg
     3841 tcagtaaatg atttttagtc acacgctcca tcgtcaatgt cagcatcaaa accaaatgtt
     3901 caagctttct atcacttaga ttggtctttt tttggatcaa ttcttccccg attactttgc
     3961 gtgcaagaga tagatgttgc ggatcaacga atcgaaaaaa ataattcagt gatgcatcga
     4021 aagaaatact atgaaaaagt tcatattcat tgatttctaa ttctaataca ttcgctgtca
     4081 gaagccggcg gtatttttcc ggtccttcga tctcgtatcc ctggttccga atgatttcca
     4141 aaggcagttg gcgaatactt tcttccaact gtttgatatc tgcataaaaa gtggtattac
     4201 tgatgcgata tgattccatg aaatgagcaa gactcaacgg ctctgtcgtc agtaacaact
     4261 cggctaaaat cgcgtgttgt cgtttagcag tggataagtc attacttttc ccatctgtat
     4321 tttcttttaa ttgctctaat acttcttctg atgcctcaat gacaaatgag cctctagctg
     4381 cttttttcaa agaagcattt accgatttca atgagacttc aagattatct aattcgcggt
     4441 aaatagtccg tttactgacc ttcaataaat tcatcatttt ttggatagat acaggtgacg
     4501 ggctactgag aaattctgct aataattgtt tttctctggg cgataaatac atcttatatt
     4561 tctcccttca cgagtttttc gtattcattc gtagctaccc aatgttcaac tagaactagc
     4621 cgagggatct tttcttctag ctgttcagtt acagctagtt ctctttgtgc gatataaatc
     4681 gtttcttctt ggattttgat tttttctatt gattcttttg agataacaaa ctgttttttc
     4741 gctttttttg ctaattgact taaaatcgtc atgcccatag tttgtgagcc gcgtctgttg
     4801 tttttataca aaaatacaat attagcaggc gacttttttt gttctgtgat atctgctatc
     4861 tctttttttt cttcccgctt tggaaattgg atcgcttctt ttattcgtga cagcaagata
     4921 tctgtttcca aaaagtgatc cagtacatag atttgacttt gaggtacttt ttctcttgca
     4981 agacccgcta gttcatgttg cacgataacc agctgattta cttggttatt caattcatag
     5041 atagagctgt atccgacaga aagatcgatt ttttctgctg ctaatttctt tttcagcagg
     5101 gcggctccca tagcacttga tcccatacct gagtcgcaag caaaaatgac ggaatgaatg
     5161 atagatggtt ccaagatttc tcccctttct ttgaccgttt tgttctcttt ttctttattt
     5221 ggtaatttca atagtatcgc tgcacaaatg aaggtcacaa atgtactgat tgctatccct
     5281 atacttaccc cagctagttg tggataaggc gcattggcaa aaatcacgag tagtgaccca
     5341 ggcgatacag gtgcagctaa tcccgtatct aaccattgaa atatcaacgt gccgcttgcc
     5401 ccaccagcca ttacagctaa aaatagatac ggtcgcagca agacaaatgg aaaatagatt
     5461 tcgtgaatcc cgccaatcgc atggatcatc aatgctccac tcgcatttac tttttcctgt
     5521 ttttctgcaa aacaaaggat agaaagtaag atccctaaac caggtccagg atttgcctcc
     5581 atcaaaaata aggaagagta cccgactgtt tgtgagaatt cgattcctaa aggtgtcaat
     5641 acaccatgat tgattgcatt attgaaaaat agtactttta gaggttctaa gatcagactg
     5701 acaagtggta acagattcat tgtgactagc caagacacaa cggaggcgag caacaagtga
     5761 acactattta aaactggttc aataaaaacg attccaatta tacaaataac gctaccagtc
     5821 agtcctacta gtaggttgct gacaagcatt tcatatccag ccttgaattt cacgtggaat
     5881 ctagaaacaa attgttcata tacgatgccg gcaagcggcc cgataatcat cgctcctacg
     5941 atttgtggag cattggaacg agcgatcaac cctaaagaag caattgcacc ggcgactgcc
     6001 cctttttgtc cccctaccga tttcccgcct gaataactga tcaagatggg taaaagcagc
     6061 tggatcatcc aatggcatac ctcttctaat gtggtgtgca aatcgccaga cacacctttt
     6121 gttgctaatg acaagattcc ccaagcgata aacagactta gatttggcat gatcatttca
     6181 tttaagtgat gtccagcttt tctcagttgt ttcatagaca cgcgactgct gttgtttgct
     6241 ttccatttgt attccatctt tttcaccata cttctattta ttgatggacg gattttatgt
     6301 agaaaaagca gcaaacgaaa aaccgtctgc tgctttcagc tatctgggaa ctatttgcct
     6361 ggttcagtcg caatgattgc tagttcacct tggaccgccc actcagaaac gccatttgga
     6421 ataatacagc ttgttccttt ttctaatgga tatgtttttc cgtcaacgac aaggtctcct
     6481 gctccttcga ttaccgtcat caatgtataa ggggcttgtt ttttgaaaga tgtgattcct
     6541 ttaatatccc actcatatac attgaaaaac tcagtttcta aataagtgac gatcgatgaa
     6601 ttcccttttc ggacttcttt gatttgtaat tctggtgttt tcgcagggac agttgttaca
     6661 tcgatcgatt gttggatatg cagctctctt gtttgtcctt gcgcatcttt tctgtcgtag
     6721 tcatacacgc ggtaagtcgt gtcagaactt tgctgtgttt ctaaaatcat gattccttta
     6781 ccaatagcat ggatcgttcc gcttggtaca taaaagaaat cacctttttt aactggtact
     6841 ttcttcaaaa ggtcatccca gcgcccatct ttgatcatct cagctagttc ttctcttgtc
     6901 tttgcatgat gaccatagat gatttctgca ccaggttcag catcgatgat ataccagcat
     6961 tcagtttttc ctaattcacc ttcatgtttc atcccatagg catcatctgg atgtacttgt
     7021 acagatagat catcttctgc gtctaagatc ttgatcaata atggaaacac tggttctgaa
     7081 ggatggccaa acaattcctt gtgttctgac catagttcat ctaatctttt ccctttgaat
     7141 tcgccatttt ctactacact gacaccatga ggatgcgcac tgatcgccca atcttctccg
     7201 attttatcgt tagggatatc aaaaccaaag actgaacgta aacgacttcc tccccaaatt
     7261 ttttcttgaa aaactggttt taaaaacata ggttcctgca taattcaaac tcctctcatt
     7321 tttcatagct attatagcat tatttcggta ataccgcttt ctctttcaaa aagttttcaa
     7381 caatttcatc tcgacatatg agatactccg cacgatgatc gacaggatgt acgcgatttg
     7441 acaaaaatat caacgcttct tgtgttttta tatcaatcaa gaaaaacgta cctgtataac
     7501 cagtatgaaa aagaatcgca tgaccgtctt ctacatcctt ttttaagtcc catcctaaag
     7561 aacgatagcc attttttttc ggtgtttgat cttgcagtaa agaatcaatc gtctcttctt
     7621 ctaaaaaacg accattacgg atttttccct tgttcaaata catttggaca aaagtaatgc
     7681 tgtctttcaa atcaataaag agacccgcgt tacctgcatg ttcagccaaa actcttgctt
     7741 tcggatcatg agtttgtcca cgcaaaacct cacccgaagg cagttgttct gtgggaacga
     7801 tttttttgga aggaaaagga agaaataagc tgttcatcat tcctaatggt tccaatactt
     7861 gttcttgaaa gatttccact gcagtttctt ggtaaatttc ctctagcatc aacccaagaa
     7921 gaatcgtacc ggcatctgta tattttactt cttttcctag ttgctcacct ggttctagct
     7981 tcaaataagc ggcgatcaat tcttcctgag atagcttatc tctgtttggt atccatgttt
     8041 tgatatcaga agtatgtgtc agtaaatgac gcaaagtgat tgtctgattt tcaaattcag
     8101 gaagatattt atgaagcggt tgatcgagtt ctatctttcc atcttctaat agacgtaaaa
     8161 ctaatgaagt ggttgcaacg actttcgtca gtgaggctgc atcaaacaac agatcttctg
     8221 tcatcagctg cttttcagga acgacttggg caaagccggc tgtaaatgtt tctacttgat
     8281 tttcttttat gaaggcgact gctacacctg gataaaatcc ttcttccatc tttttcaaaa
     8341 taagctgttt cgtgtgtgga tacatctttt tctcctgctt ttctatcaga ttcttacagt
     8401 ccaagcttaa agcttaatct atgaggaatc aacctttttt acacaaaaaa actgcgcgaa
     8461 aaaccgcgca gtttcccccg gtatactatt ttctctttct atctctagaa taagcgatag
     8521 tatgaccgtt cataaaatga cgagacatgc gacaatcgtt aagaaagaaa aagttaagcg
     8581 tcgcatcgaa tgtcagtttt attcttttct tttggctcac tgacgaacca ccattcgatt
     8641 cctatagcat catagattgt gagtactgtg tgctttttgt ctaagaagaa atcaactgat
     8701 agttgctgca agtgagcaat cagtccttcc atgctttcag tatcgatcat gaatttcaga
     8761 aaatcaagtc caatcacttc atctgtaggc aaatcaattg cgcctccgct agcttctgtc
     8821 aaaccaacat ggaaatctcc ttcattcaat acatggatgc cctcttcttc attatgtacc
     8881 ttcaaaccaa gtacatgatt aagaaattgg cgttcttttg ttaagttagt aacatttaaa
     8941 tggatttttt caaaataaga acctgttgat agtttactga atttttcttc agattttctt
     9001 aataatgctt cttgatccat tgtttctgga atttctcttt ggtcgttctt attctcttca
     9061 tggtaaattt ccaattcatt ctcttctggg tctacgagca aaatccccat acgtccatga
     9121 tcttctaaag cttctttgat tggataattc attttacgga tacgacaaag gatatctgcc
     9181 atttcttctg tgctagggat aatcaatgaa aaacggttca gctttttgat cacccccatg
     9241 tgatcattcg ctctaggact ttcttccagc agcaacattt cacttccagc ttcttctgtt
     9301 cctaaaatgg ctaattcgtt ttcttctctt ttcaaatcaa agccaacaac gttcttgtaa
     9361 aaatcaatca ttttatctcg gtctttcacc cgaatggcta cactttttaa ataggtggca
     9421 gcccccaatt gaaaatcttt catatttctt gctcccctct acgtcaagct ttattttaac
     9481 gatggattct tcttctttca agcaataact ttgattttgt taaaaaaaat gtgagaaaat
     9541 tcgcattttg cttgtttttt gtaaattaat gagtaaaaac tcttataaaa agaggttgtg
     9601 aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt
     9661 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
     9721 tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat
     9781 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa
     9841 aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat
     9901 tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt
     9961 tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa
    10021 tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt
    10081 ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg
    10141 ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta
    10201 gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt
    10261 ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt
    10321 cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc
    10381 aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg
    10441 tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt
    10501 tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt
    10561 tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat
    10621 tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg
    10681 acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga
    10741 tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca
    10801 agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta
    10861 cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg
    10921 aaataaattg agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt
    10981 cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt
    11041 ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt
    11101 tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg
    11161 tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag
    11221 ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg
    11281 ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca
    11341 ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa
    11401 taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa
    11461 tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca
    11521 tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct
    11581 ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg
    11641 caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag
    11701 gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg
    11761 gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga
    11821 atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc
    11881 aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg
    11941 ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt
    12001 ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt
    12061 tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc
    12121 tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg
    12181 tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat
    12241 attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc
    12301 tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga
    12361 ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc
    12421 ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag
    12481 tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc
    12541 agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt catccaacac
    12601 ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag
    12661 catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac
    12721 ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc
    12781 gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc
    12841 cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc
    12901 gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc
    12961 agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa
    13021 aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata
    13081 agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat
    13141 ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt
    13201 tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg
    13261 caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc
    13321 taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac
    13381 atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc
    13441 tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg
    13501 ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca
    13561 agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga
    13621 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
    13681 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
    13741 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
    13801 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
    13861 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
    13921 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
    13981 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
    14041 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
    14101 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
    14161 atggaaaaat gtctgtgtcg gcatatggat cctcctttct ttcaagatga cacattgtca
    14221 tatgacgctg tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
    14281 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
    14341 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
    14401 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
    14461 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
    14521 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
    14581 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
    14641 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
    14701 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
    14761 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt ctcatagtta
    14821 taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta gtagtcaaag
    14881 gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct tttttaacaa
    14941 gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat cgcgatgata
    15001 ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct ggtgcagaat
    15061 tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa ttgactgatc
    15121 agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta aatatcccaa
    15181 caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc cgctatacag
    15241 aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa tctaatggcg
    15301 gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta aactttgtag
    15361 gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct gaccgtgcag
    15421 atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca ttttaaaaat
    15481 tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca tttgtacctt
    15541 ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg gattttcttc
    15601 ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta ctttagctgc
    15661 tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc cagaagtcag
    15721 cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta ctaatggaga
    15781 gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt tgatgatcat
    15841 gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa atgatcctaa
    15901 gtaagggatc aggttcaaaa aacccgcaat aatccccaac gtgacagcat actctaaacc
    15961 aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag ttagttgtcc
    16021 tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg tcggtttttg
    16081 catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt ctttcaatag
    16141 ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta ccgcgccgac
    16201 aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat cgcctgccga
    16261 attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga acagcgggtc
    16321 ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga agttactgat
    16381 gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc atgcgattag
    16441 tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt ttcgcttttc
    16501 aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa tgataggcag
    16561 acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa
    16621 aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt gattatttaa
    16681 aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt ccataattcc
    16741 tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct atgcggatga
    16801 agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat caatgatacg
    16861 tgagctactg tctctaaatt cccattggcc atcggaccga cataatcgat cgtatatacc
    16921 tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact
    16981 tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc
    17041 atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc ctgaatcatt
    17101 gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc tttagccgtg
    17161 tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta ggaggaatca
    17221 gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag taaaggaatc
    17281 tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt agctgttgaa
    17341 gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac agttactagt
    17401 gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt gaatgcggta
    17461 acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca attactatac
    17521 ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga tggcgaactg
    17581 aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat
    17641 gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg tgatcttggt
    17701 acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct tgtaactacc
    17761 ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa tggacaattc
    17821 gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa taaagaaaca
    17881 ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga cggtgaaaat
    17941 ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc taaccgagga
    18001 cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca aaacaagcaa
    18061 atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag caataatttt
    18121 gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca tcctgaaaca
    18181 ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt
    18241 gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga cagactacgg
    18301 gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta tagcgaagag
    18361 gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt acagaatgta
    18421 gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac ttttccgtct
    18481 atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc gacggaaaag
    18541 gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt atgtcattat
    18601 cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt agataactct
    18661 agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag tcattttttg
    18721 gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag
    18781 atcttcatct acccctgatt caagatcgat actttggatc atttcttcac agcctgctac
    18841 atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg ctggtggacg
    18901 caaagtactg actttcttaa gcattgcccg atattttttt gttccttctt caaataacag
    18961 gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat caattgcgtt
    19021 tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt ccttgacgat
    19081 ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt tcctactttc
    19141 tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag tacttgaaaa
    19201 aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta
    19261 aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg tcgtaaatct
    19321 cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga tagtacagtt
    19381 ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa aagccatttg
    19441 ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg gtgattgcat
    19501 cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat tgtgtttcat
    19561 gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct ggaagatgat
    19621 ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc gtgaactgct
    19681 tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca agtgtcccac
    19741 ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg tagggcatat
    19801 gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg agaagtgtca
    19861 ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc gtatccgtat
    19921 aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa acatcctctc
    19981 ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta
    20041 acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg taaggggtgg
    20101 tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg
    20161 ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga tcagctactt
    20221 cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga ctaggatctg
    20281 agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca gttttatttt
    20341 tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct ttcgaatatc
    20401 cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg ataaaaccag
    20461 tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta ttatatgctt
    20521 ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt ccaattgcca
    20581 caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg cttcgaaaca
    20641 tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact gtaaacagca
    20701 tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa tcgtctggat
    20761 aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat acaccgatgg
    20821 ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc agaaaaataa
    20881 gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa gaaaaagcaa
    20941 atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat aaattactaa
    21001 aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt
    21061 tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc tatcaataaa
    21121 agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga
    21181 attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt aataagcgga
    21241 cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc tgttcaaaat
    21301 cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta cgcgatatat
    21361 ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat atattggttt
    21421 tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt gcggatggat
    21481 aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct tcaactgcca
    21541 aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc aacataagta
    21601 tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta gccagatgtt
    21661 gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt cctacattga
    21721 taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat ccttttgtat
    21781 cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact gtttctaaat
    21841 ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag acatccatcc
    21901 ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca agaagaccga
    21961 tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct gcctgttcta
    22021 aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc ggacgggaag
    22081 ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca cctggtgtat
    22141 tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc accccaacac
    22201 cagctcttga aattgctagc aaatctggtg taagccattc atctttcact ttcgtactac
    22261 gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc ccttgtaact
    22321 tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata aaaccccctc
    22381 cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat ttgacttcct
    22441 agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag gctatgacaa
    22501 gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata tcttgataaa
    22561 tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt gaaaagcaaa
    22621 tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc tctttttatc
    22681 taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga tctgttgtta
    22741 gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa tggacacctt
    22801 catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta ggtgcttcag
    22861 tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta ccgaaatctg
    22921 ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc gccggatctt
    22981 ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg
    23041 gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct agctcgtaag
    23101 tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc aaccgatcaa
    23161 tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc atcaaacgtt
    23221 gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag
    23281 cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga gcttgtactt
    23341 ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta tacaattcct
    23401 tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt acttgtacaa
    23461 gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct tccactttgt
    23521 ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact tttattccaa
    23581 aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt tggatctcag
    23641 gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca ttgatgaacc
    23701 catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata tcgatcgttt
    23761 tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt tgtagaagct
    23821 ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg atcacacctg
    23881 ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca gcagtaactg
    23941 ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt tcatcgacca
    24001 tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa tgctgtgatc
    24061 ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac tccttttatt
    24121 tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta tctaaaatcc
    24181 gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc atcttgcata
    24241 cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc caagtaaatg
    24301 gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg cgttctttga
    24361 atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt tcctgttgtg
    24421 tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc gtccccatcg
    24481 caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga gtatggacga
    24541 ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta tccgaagagg
    24601 gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg tcgtctgctg
    24661 tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt tcaggatcaa
    24721 aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag tcaagatttg
    24781 cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt ttcttgcaat
    24841 tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat aaatatctga
    24901 tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg tccaaacacg
    24961 tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata ggctgctaat
    25021 aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac tggtgcctcc
    25081 atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc cccatgcccc
    25141 actaaacgat caatttggac attctcagat ttcaatatat ccattccgat tctcattgca
    25201 ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg gctacctggt
    25261 gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc ggaaaaataa
    25321 ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt gaataatacc
    25381 gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc ttcaaaaata
    25441 ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc tactagttct
    25501 cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc tttttctaat
    25561 acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtccgttt ggctacactg
    25621 ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg gattcctggt
    25681 ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt gccagcttct
    25741 tctcctgata ctagaacttt aggcaaaatg tgatccaagt caatcgctac gtgcgctttt
    25801 tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt tgaaggatcg
    25861 ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt aagctgccaa
    25921 tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc ttctttattc
    25981 aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta ttgaaatttt
    26041 tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg aaatggtact
    26101 agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact gaacccaatc
    26161 gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc catttttcgg
    26221 taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat accattttct
    26281 agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga atcaatcaat
    26341 acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc atctatgaca
    26401 tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc ttctcgtcaa
    26461 acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact tttgtacgta
    26521 catgtcaagc ggttatttac aagaatacaa taaaaaacac ttgttttgta cgtacataag
    26581 ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt ctagaaggag
    26641 agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt ttttggcgca
    26701 tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt acctttttta
    26761 cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac gtcttcggtc
    26821 atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa gcttggacga
    26881 cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct ttctgcatct
    26941 gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg tttagctgtt
    27001 ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc tagactaagg
    27061 ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct ttcctatgtt
    27121 gcagattatt tgctaaaagt gttgcctgaa acgatggctt ggcgcgtgat gttagggctc
    27181 gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga gtcaccgcgt
    27241 tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta tattcggaca
    27301 ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca agaaaagaca
    27361 accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt cattgccggt
    27421 attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta ctatattccg
    27481 ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg gccgatcatc
    27541 caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga caagttcaat
    27601 cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttcattcat cctaccagct
    27661 gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt gtttttatgt
    27721 atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat cataggcgaa
    27781 atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt taattggatt
    27841 ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc tcaagaaatc
    27901 gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca agagatcgta
    27961 ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa aaaaacttat
    28021 cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg tcagcttcaa
    28081 gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa aacagcttct
    28141 catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt ttagtggaga
    28201 ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa cattgcgacg
    28261 agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc gttttctttc
    28321 actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc ctgtcctttt
    28381 tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt tggatgcgtc
    28441 aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt tcctacgatc
    28501 gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat taaactagct
    28561 atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa ccgttgtact
    28621 gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc ttccgtcaaa
    28681 aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta ttttccttga
    28741 agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat caaatattct
    28801 gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt taactcttca
    28861 taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac ataaagtgct
    28921 aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc taccccataa
    28981 gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt actggctaaa
    29041 agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat cgacggaaaa
    29101 atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt caatggtgtt
    29161 ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt acgcagatag
    29221 ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac gtcatatatt
    29281 tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc acgaaggatc
    29341 ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc ccctttttat
    29401 ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa tcaaggtttc
    29461 ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt gctcattcca
    29521 cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc ctttttggtt
    29581 cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg aaaccgtaaa
    29641 cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata ctggctccga
    29701 taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc acagcaggcc
    29761 catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt tcccattgcg
    29821 agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt catactcagg
    29881 tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt cggatagata
    29941 aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat atagctttcc
    30001 ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt ttatttgtcc
    30061 cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta cgaacatgcg
    30121 gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt aaatatacca
    30181 ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg gtccttcttc
    30241 atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg actttgccct
    30301 tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat aaccatctgt
    30361 atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat aaagtgtctt
    30421 catcataaca aatttcctca cattttcttt attctaatta cattccaaga gtatttttct
    30481 atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc aggttttatt
    30541 ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa tgtgtagtgc
    30601 tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg ttcatttgtg
    30661 cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact gtccacataa
    30721 ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg tttcaatact
    30781 tgacctttag catatttcga taagtgttgc atcacataat aagttggatt cctccatgct
    30841 tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga ttgacaacta
    30901 atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa taataatgta
    30961 tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc cctatgtctt
    31021 ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt tttctcactt
    31081 ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg acgatcgaat
    31141 cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg aggtttagtc
    31201 aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc tacattctca
    31261 taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg tggcgtagag
    31321 cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt tgaggcttca
    31381 tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg accgtctagc
    31441 tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt ttttattctt
    31501 aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt ttttgcttca
    31561 tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac atgactgatc
    31621 catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca tgctaaatca
    31681 attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata accagatgta
    31741 aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata agagaggaca
    31801 tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat ccctgtatag
    31861 acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat ctctcctacc
    31921 gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc ctacctttca
    31981 tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa gataaaaata
    32041 aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca acaaagaata
    32101 tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg taaaaatgtg
    32161 atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga ataaataagt
    32221 aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa ttgattaaca
    32281 agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa atgttgcttt
    32341 attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca ttgcgccatt
    32401 acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca cacgattacc
    32461 cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata aaatttagtc
    32521 tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct tgaacaaaat
    32581 atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc gttccagcca
    32641 ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct gctggtaaag
    32701 ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc caactattca
    32761 tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac atgattttca
    32821 aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta
    32881 aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata cgaggaatga
    32941 tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat agaggaatca
    33001 tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa actgattttc
    33061 ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat acagaagtga
    33121 tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa gggatcgttg
    33181 tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta ggcggaaatg
    33241 agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa
    33301 taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca ctaactgctt
    33361 ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt tccctcctat
    33421 ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat agttgcaaca
    33481 atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg tgctgtaaat
    33541 cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc accagcggtt
    33601 gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa aatacaaaaa
    33661 cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg atttgttgca
    33721 ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc agtcaagaga
    33781 atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt cggcatagca
    33841 agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa caagtaagga
    33901 atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa
    33961 caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt atttttagat
    34021 aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag tacggtgaaa
    34081 tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg tgtgaaattt
    34141 tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa actaatagca
    34201 aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga tggagttaaa
    34261 aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt catatagtcc
    34321 acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct gaataagata
    34381 tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag gtcgatcatt
    34441 cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta tttttgcagt
    34501 aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc tcctgctaat
    34561 aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg ggtaaaacct
    34621 ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat aggatcattt
    34681 tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt ttcgatactc
    34741 cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac agcttctttt
    34801 ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat agctgttttt
    34861 ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc gtttgctttc
    34921 aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc attcattaaa
    34981 ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg gatcattttt
    35041 tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt agtataatca
    35101 tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc agttccatcc
    35161 ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg tgccatttct
    35221 gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg tttaaacaaa
    35281 tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata ctgtttacct
    35341 tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac tccatcatat
    35401 tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc aaaatctgga
    35461 taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg aagtttttca
    35521 ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc atttgtttta
    35581 ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa cccattccaa
    35641 aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc accgcaagct
    35701 cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt acctttcatt
    35761 ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata gcggtttcaa
    35821 taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg gttttacttg
    35881 ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat
    35941 cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt cttctccacg
    36001 gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt ctaaatacgg
    36061 cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc ttaacacctc
    36121 tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc tccaagcttc
    36181 tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc aattgagcaa gacaagcaat
    36241 ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata gtaatgcatc
    36301 ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc
    36361 atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt tgatctgctt
    36421 cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag aaatgaaccg
    36481 atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt tgccataata
    36541 ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg ttaactccca
    36601 atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct caattgaatc
    36661 atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt cttccgctgt
    36721 tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg tcttaattcc
    36781 aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc ctttaggaaa
    36841 attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca gattgacagc
    36901 catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa tcccaacttc
    36961 attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg ctatgggccc
    37021 aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc taatcatggg
    37081 aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt tattagctga
    37141 tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt caataaatga
    37201 actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca acattgtggc
    37261 ttccataact gacctccttt aatttataca tgaataatac acctcgttga aagcgttg
//
LOCUS       DACCVU010000015        37245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCVU010000015 DACCVU010000000
VERSION     DACCVU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.015"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..839
                     /locus_tag="IVW29_000647"
     CDS             <1..839
                     /locus_tag="IVW29_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7838150.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="IVW29_000648"
     CDS             complement(1263..1433)
                     /locus_tag="IVW29_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7838151.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="IVW29_000649"
     CDS             complement(1627..1806)
                     /locus_tag="IVW29_000649"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838152.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="IVW29_000650"
     CDS             2193..3305
                     /locus_tag="IVW29_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7838153.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="IVW29_000651"
     CDS             complement(3383..3676)
                     /locus_tag="IVW29_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7838154.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="IVW29_000652"
     CDS             3844..4158
                     /locus_tag="IVW29_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7838155.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="IVW29_000653"
     CDS             4179..6158
                     /locus_tag="IVW29_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7838156.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="IVW29_000654"
     CDS             6171..6497
                     /locus_tag="IVW29_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7838157.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="IVW29_000655"
     CDS             6513..7031
                     /locus_tag="IVW29_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838158.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="IVW29_000656"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="IVW29_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7838159.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="IVW29_000657"
     CDS             complement(8467..9972)
                     /locus_tag="IVW29_000657"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7838160.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="IVW29_000658"
     CDS             complement(10064..10654)
                     /locus_tag="IVW29_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7838161.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="IVW29_000659"
     CDS             10833..12479
                     /locus_tag="IVW29_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7838162.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="IVW29_000660"
     CDS             12528..13247
                     /locus_tag="IVW29_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7838163.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="IVW29_000661"
     CDS             13726..14673
                     /locus_tag="IVW29_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838164.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="IVW29_000662"
     CDS             14685..15605
                     /locus_tag="IVW29_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838165.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="IVW29_000663"
     CDS             15646..17103
                     /locus_tag="IVW29_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7838166.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="IVW29_000664"
     CDS             17448..18062
                     /locus_tag="IVW29_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7838167.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="IVW29_000665"
     CDS             18055..19779
                     /locus_tag="IVW29_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7838168.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="IVW29_000666"
     CDS             19776..21227
                     /locus_tag="IVW29_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838169.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="IVW29_000667"
     CDS             complement(21700..23853)
                     /locus_tag="IVW29_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7838170.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="IVW29_000668"
     CDS             complement(23855..24901)
                     /locus_tag="IVW29_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7838171.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="IVW29_000669"
     CDS             25051..26340
                     /locus_tag="IVW29_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7838172.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="IVW29_000670"
     CDS             26353..29055
                     /locus_tag="IVW29_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7838173.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="IVW29_000671"
     CDS             29040..29897
                     /locus_tag="IVW29_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7838174.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30825)
                     /locus_tag="IVW29_000672"
     CDS             complement(29968..30825)
                     /locus_tag="IVW29_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7838175.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31036..33207
                     /locus_tag="IVW29_000673"
     CDS             31036..33207
                     /locus_tag="IVW29_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7838176.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="IVW29_000674"
     CDS             33275..33616
                     /locus_tag="IVW29_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7838177.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="IVW29_000675"
     CDS             complement(33892..35646)
                     /locus_tag="IVW29_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838178.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="IVW29_000676"
     CDS             complement(35657..36493)
                     /locus_tag="IVW29_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7838179.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="IVW29_000677"
     CDS             complement(36495..37178)
                     /locus_tag="IVW29_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7838180.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6552 c         7637 g        11040 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa tggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaactcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DACCVU010000016        36639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCVU010000016 DACCVU010000000
VERSION     DACCVU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.016"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..267
                     /locus_tag="IVW29_000678"
     CDS             <1..267
                     /locus_tag="IVW29_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838181.1"
                     /translation="SVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLK
                     DVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            280..540
                     /locus_tag="IVW29_000679"
     CDS             280..540
                     /locus_tag="IVW29_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838182.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            537..2627
                     /locus_tag="IVW29_000680"
     CDS             537..2627
                     /locus_tag="IVW29_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7838183.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(2663..4720)
                     /locus_tag="IVW29_000681"
     CDS             complement(2663..4720)
                     /locus_tag="IVW29_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7838184.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(4865..5056)
                     /locus_tag="IVW29_000682"
     CDS             complement(4865..5056)
                     /locus_tag="IVW29_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838185.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            5244..5699
                     /locus_tag="IVW29_000683"
     CDS             5244..5699
                     /locus_tag="IVW29_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838186.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            5696..5938
                     /locus_tag="IVW29_000684"
     CDS             5696..5938
                     /locus_tag="IVW29_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838187.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            5956..6258
                     /locus_tag="IVW29_000685"
     CDS             5956..6258
                     /locus_tag="IVW29_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838188.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            6327..8381
                     /locus_tag="IVW29_000686"
     CDS             6327..8381
                     /locus_tag="IVW29_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838189.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            8381..10993
                     /locus_tag="IVW29_000687"
     CDS             8381..10993
                     /locus_tag="IVW29_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7838190.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNEEISEMI
                     GKRLLLESEKVVKE"
     gene            10990..13395
                     /locus_tag="IVW29_000688"
     CDS             10990..13395
                     /locus_tag="IVW29_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838191.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDVTPDNGEEQPDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTD
                     PEQYRYRSVNSENEKRISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQ
                     NLPMKDSRLNQFKENDQSFVHQSPAGTVQMKPSRLPENGNLTTVSYDLSESGETGSAS
                     VQNVVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQD
                     IKP"
     gene            13447..13779
                     /locus_tag="IVW29_000689"
     CDS             13447..13779
                     /locus_tag="IVW29_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838192.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            13780..14373
                     /locus_tag="IVW29_000690"
     CDS             13780..14373
                     /locus_tag="IVW29_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838193.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            14536..16230
                     /locus_tag="IVW29_000691"
     CDS             14536..16230
                     /locus_tag="IVW29_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7838194.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFVKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAESHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            16918..18795
                     /gene="ltrA"
                     /locus_tag="IVW29_000692"
     CDS             16918..18795
                     /gene="ltrA"
                     /locus_tag="IVW29_000692"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7838195.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            18926..19303
                     /locus_tag="IVW29_000693"
                     /pseudo
     CDS             18926..19303
                     /locus_tag="IVW29_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            19362..19625
                     /locus_tag="IVW29_000694"
     CDS             19362..19625
                     /locus_tag="IVW29_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HAP7838196.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            19625..19960
                     /locus_tag="IVW29_000695"
     CDS             19625..19960
                     /locus_tag="IVW29_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7838197.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            20034..21236
                     /locus_tag="IVW29_000696"
     CDS             20034..21236
                     /locus_tag="IVW29_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7838198.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDPL
                     PFLNSSKK"
     gene            21252..21896
                     /locus_tag="IVW29_000697"
     CDS             21252..21896
                     /locus_tag="IVW29_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838199.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            21907..22713
                     /locus_tag="IVW29_000698"
     CDS             21907..22713
                     /locus_tag="IVW29_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838200.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            22736..22966
                     /locus_tag="IVW29_000699"
     CDS             22736..22966
                     /locus_tag="IVW29_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838201.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTAERK"
     gene            22984..23223
                     /locus_tag="IVW29_000700"
                     /pseudo
     CDS             22984..23223
                     /locus_tag="IVW29_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23230..24102
                     /locus_tag="IVW29_000701"
     CDS             23230..24102
                     /locus_tag="IVW29_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838202.1"
                     /translation="MKSSDWMEHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLFTIFTQIAMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGLKEFLMTFVHFVNGYMYLFEVGIILF
                     LFGYISTKLIKRSIEKEIEIDLIPTLFGYEYYVDFGRYDELINTGIVHTSIYEATVKG
                     IRTTLKDGQESKAIDRRKEVYNFYEVEASLRYGIGKLSLDGRNIPQNKPYIGWKSDAL
                     EVSKKLSMEEDELQ"
     gene            24099..25496
                     /locus_tag="IVW29_000702"
     CDS             24099..25496
                     /locus_tag="IVW29_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838203.1"
                     /translation="MSSINLNHLFEDISNHFQQFSIEDNDELMGLFQIEKEYDYLTFI
                     CREGRIIDPKESKIISHLRRFKNYISKNKETISEIKFDGTTNLEYLVRVTYQRNRRLI
                     EDDQGVLICAGEEYDSLKFTQLKFESIGRSIYFESRPFYIRYNDFQHLFLQDEPNGLT
                     ILERRGKKQITLSYERPLTFMDGSCESIFQKINPFIKEMMKEKDSLPFTWAEIIEAKN
                     KYELLCKKYPSKKFTKKVNKYPLRHTYALIKLKPRVTRKQFRRIEAAIEQEDIDVFPS
                     ERFDFIRKNSREFVKSLLISYVQNCILPSFATIILSDLMDMSFYLNQKLEFNFKTERG
                     LLRQHNQIVEEYNLKQAKRLKTFKLKQHGKYRLLVKHLQNHPHFNLIKTNKELFMEGS
                     TMHHCVYNYLEKIQRGGSTIWKYERQKHRYTVEIEKRKYGNYEVVQCYGKYDSLPDKQ
                     ELDEIIKVVRAIPYK"
     gene            25729..26037
                     /locus_tag="IVW29_000703"
     CDS             25729..26037
                     /locus_tag="IVW29_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838204.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            26039..26461
                     /locus_tag="IVW29_000704"
     CDS             26039..26461
                     /locus_tag="IVW29_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838205.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            26481..28070
                     /locus_tag="IVW29_000705"
     CDS             26481..28070
                     /locus_tag="IVW29_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838206.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLNEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            28082..28417
                     /locus_tag="IVW29_000706"
                     /pseudo
     CDS             28082..28417
                     /locus_tag="IVW29_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28565..28759
                     /locus_tag="IVW29_000707"
     CDS             28565..28759
                     /locus_tag="IVW29_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838207.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEVESERTFVVTDKEAKAYQPGDL
                     LTLDLTTKKFVDAAENYPFI"
     gene            29168..31555
                     /locus_tag="IVW29_000708"
     CDS             29168..31555
                     /locus_tag="IVW29_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838208.1"
                     /translation="MPEEVKGKSSLTLNEEEITGNSRLAQAQRKLIRLRNEYDATFER
                     LAEHQKLTNGQPMNDKRNGSSWFSQREKIENKINQLQEEIQKQENYVEKLTRQSELRK
                     MGYNKYGGLDLTIENIPRIKEEIEKFRNGKSQFSVKTIEKYQKKLVELEQLKEKSEKG
                     IANILPEAQAIIESGRVSQWKRNPTIYFVKGYRKVALELNEQGEFEVSKRYQPKTEEE
                     KRIVSELLENKMDEYGVQKQEPNHYYRVEFNENDGTMRNYQGEIVTQSLLEQIKALDT
                     HYIGKTSYHKFYFEEVKEGEVVNQLRLDVGDGLQLNASIYQKLEELCDQTLETKTEKV
                     IKNEIGSHDKKVETKKPSYQKRSTEEIKQEVKLLSQQALDAVKKYTHSPEDVKELLNF
                     MSRFPERSFRNQLLIEKQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYD
                     KGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRV
                     FDFQLDKKGQAELQAGIQAVANTIGIQIRDMTESEVYQRELGQARGAYVHNEFTGQEE
                     IVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEK
                     AIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPA
                     EVKQEHKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            31616..33076
                     /locus_tag="IVW29_000709"
     CDS             31616..33076
                     /locus_tag="IVW29_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7838209.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRKKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYILKDESKVERVEN
                     LHFETQEYKHHPMDYQPLTEKGRTYLKEQRKFPEWLIEYGDQQGLILEMKPRSERKNY
                     IVKDDRLDYAIVFPWKDRHTDQVLGATYQGTIIDYERFGDRGTYKHIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYVALNRETVKDTWLISMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHIFWDKVHRLGVSNPFTGKAVYFEPDLPNDWQVPRKYQTI
                     YEEVGKEMNVAPEAIMAIHKIENNLTENNQIVLFGEIHGVFAKKLASKEKVQIIDVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEEIQISLKAKHYFEGYKNHDHEFVSE
                     VKKDWNDQWKHEIQQQEIRKQKRAMLFQQSRQQERE"
     gene            33087..35291
                     /gene="topB"
                     /locus_tag="IVW29_000710"
     CDS             33087..35291
                     /gene="topB"
                     /locus_tag="IVW29_000710"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7838210.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGAEQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNDKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKCWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            35532..36125
                     /locus_tag="IVW29_000711"
     CDS             35532..36125
                     /locus_tag="IVW29_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7838211.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMGDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            36138..>36639
                     /locus_tag="IVW29_000712"
     CDS             36138..>36639
                     /locus_tag="IVW29_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7838212.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPT"
BASE COUNT        13506 a         5703 c         7524 g         9906 t
ORIGIN      
        1 tcggtagaag tacaggcaaa tcagaaacaa gcttattata ttgtcaatga atcaagtcct
       61 gaaaacattt ctaatagcga ggatattttg ctattaacac ctgtttctga taaaaatggt
      121 gaattcttaa aggatgtgtg gatttatcct aaatcagaag cttcgcaacc taaagaagaa
      181 gtaaaaaaaa tcgtatctac tggcgtaaag aaaaacttct ttgagaattg ttgggatttt
      241 gttactcact tgttttctag agattaagga agaaataaaa tgaaactgat caaattatta
      301 ttattcaatt tttcttgttt aaccgcagtc tttttgtact gcggtttatt attttctaaa
      361 ctatctattt tgccaagcta ccaactatac ctaatgagga ttttaaacat accagctgct
      421 tccatgccgc tagatattat ggaaaagctc attaggaatc cattatttac tacgatcaat
      481 ttgatcgtta ttgtcggaat ttcactagtc atttataaaa aagaggggat gaagggatga
      541 attttaaaca ctcgtggaca agacggttaa aatatgtttt tcccataatg atgatgttag
      601 gtacattgtt tgggttaaaa acagccaatg tctttgctga aaaagttatt gtcgatccgt
      661 caaatccaat ccagaacgta aagaataatt ttattattcc aaaagatgtt cctaatgcaa
      721 aaacaaatat tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca
      781 aaggtggttt tacagacgac tacgtaggat tagtggaggg aaacaaaaat atacgctatc
      841 cttctttcca aaaagaatac ggtgaaacaa accttgtttt agaaggcgta acaaccaaca
      901 ccaaagtcaa tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca
      961 agctagttct atctaatatt cacttatact cggatacgtt gccgtggaat ctactagata
     1021 acaactatac aaaaactcat tttcgagacg atggctataa aaatacgaat ggtgcaatga
     1081 gtaaatctaa aaaaagaaca gtactttgga tctcagacaa tcttttctca ggaattgttt
     1141 accattcaac acaaatgaat gtgcagctag tagccacata tgaggacggt agccccgttc
     1201 aattttcagg agatacattt atcagtttta actctttgaa ccctgcagga ggaaaaagca
     1261 cggatcttaa aggagaatat gcccactatg acaaaatgaa tacgacagat tggtatgtca
     1321 gaaaagatac ggttctttca gaatttaaat ctttctacaa caatctgaac gtggtaggtg
     1381 gccacccagg aggaagctct aagctgacac aagcggataa tgatttcaat aatttgcatg
     1441 ataaattagg agatccgaag tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa
     1501 atccgacgtt tgtgattggt tcttctaatg ttcaaacctg gtttacttta tcttctgcga
     1561 caatctttag tgttgttcct gatcaacctg aaaaaacagg ggttgataaa aatggaaata
     1621 acgtgaacga taaaatgttg caagtaggag acacgattca atatcgaatc aaacagaagg
     1681 tgaatcgatt aggagtggat ttactagcgg tttacgatcg atttgagtta atcgataatt
     1741 taccaaaaga agtaaattac gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg
     1801 atgtatctgg tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag
     1861 ctgatacatt aaaaaatcga atgaagtata acggcgaaac ttacgaatta gtgatcaatg
     1921 taaaagtcaa tgaattggcc aatcaaaata gtgtagccaa aaatcaggga acgtcgatca
     1981 ttaataaagt agaaaaggat acaaatatca ttaccgttta tttccctaaa attccagtga
     2041 aagaagtaca acaaaatgga aaagatgtaa acggacgaaa tgacggtaag aagggtacgc
     2101 cgacagctcc tttaaacgca ggatctgaag tacaatatct agtcacacaa aaatggcaca
     2161 caaaaggcgt agacgctgcg tcagatcact acaaacaatt tagtattcaa gatccaattg
     2221 aagctcgttt gacatataaa gaaggttctg cgcaagtaat agataagagt aaaggaaaag
     2281 atattacttc tgaaggcaca ctaacctatg atagcaattc aagaacgctg aagtgggaag
     2341 cttccgctga cttcttaaat aacaatcttt tagatggacg agaaattcag ctgatattta
     2401 cagctaaaac tccattacaa tcagaaaaaa atattgataa ccaagccgta gtagcagtag
     2461 ataatgtttc taataaaaca aatgttgtga caattggggt tgatcctaac ttgccacaag
     2521 ttattgttcc taaaacaggt tctacacatt tagtaacgat tttagcggtc agcttactat
     2581 tacttgtgtt agcaactttt ggctacgcag ttctgaagtt caattaaaca aaaaaacaag
     2641 gctgaaaatc agccttgttt ttctatttga aaatgggatt ggcactaata aatacgttgt
     2701 ctgaagaaag tgatccgtct gcagaaactt cataagcatg ggataaacca acgatacttt
     2761 gattgttgta aagcaagttt gcgttatgag cagcgctatc tttccacata accatagcat
     2821 ttactgctcc tgtatctgaa aggatctcgt caacgtaaag gtagaaacta ttgatatttt
     2881 ctcctttagc accgtagcag cctaagcttt ctaagagatc ttggaaagta ccgccgccag
     2941 gtcggtaatg actgaagctt acttgtaatt cttttgcacg aatatctgca gcttgttgaa
     3001 cgatatcatt gaattgcatt ggggcaacgc cattttgttg acggtagcta tttaaccaag
     3061 caagttcttt ttgacgcaca tagttgttta aatcacttag tgagatattt ttgtatttga
     3121 aagtgatcgt tcgattttgg tcaacattat tgattacacg ttgtccattt ttatcaacgg
     3181 taacagatga atcgtcattt ctacacaaga agtagttttg atcggtcata tttttccaag
     3241 aagtagagaa actatctccc tcgtaaaccg tttgtgtttc agtagataaa acagcgtttg
     3301 tgtcaacgtt tatatggttc acagtgacgt tgtattgatg agtataggca aaattgattc
     3361 cttgattact tgtaacgttg tttaaagtca cagtgttgct agagctttct ctaggatcaa
     3421 gaacaaaatt agtgaacata ggtgcttcaa ttgtgatgct ttgtccataa acgacgggat
     3481 aactcttact gtaattatag agttcttgga tatttggatc agtatccgag actttatttg
     3541 gacgagcttt cacttcaact agaaagactt catcatagta aaaagtaaat gtggtatttg
     3601 tgttaatcgt ttgtgtttgt gtttcattcc catttacttg atactgtggt gcttcaaagt
     3661 tagtcaagtc ggttctagct tttgcagtgt aacttgtacc agcttttact gagacatgtt
     3721 cttcttttag cagcgttcca gtttgtcgat tcatatgacg gacagttacc gcatattctg
     3781 aaggtttagg tttgtgttgt aaccctttaa atgcagaatc aagactattt ttagcattat
     3841 ttacttgggt ttgagaagct ttagaatcgt ctagtacttt tttagcattg gataaagcag
     3901 tctgaaaatt attccaagat ccttctgtaa aatctccttt agcagttgat tttacttggt
     3961 tgtacaaggc ttgtaattcc gttttgttca cagtaggagc tggttttttc tttaatccct
     4021 tgtaagcaga atctaatgtg tttttggcac tatccacttg tttttgagtc gcttttgagt
     4081 catttaacac ttttttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg
     4141 tatagtcacc ttttttagtt cccttaacgc tcgtataaag agcttctaaa gctgatttat
     4201 tcactttggg tttagaggtg tcagcaccat accaaccaat accttcgtat ttccagccat
     4261 agtctttggt taaccattgt acttctttcc aacttgcagt gtagtgatgc gaaccagagg
     4321 taacacctgg aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg
     4381 caggcccttc ataagtccaa ccgtaatttt tggtcaacca tttcacttca tcccaactag
     4441 ctgtgtaatg gtgatcacga aggcctttat tgtataaacg atagacaagg tggccaccgc
     4501 tagtcggtgc ttcccaagcg gggccttcat aattacccca cttaattttc actaggtgat
     4561 cacgttcttt tgtgctagag gtgtaaaaat gttcatggtt atttggatta taaaggcgat
     4621 acatgacgtt agttgaagca gcgtcagcag atccagcaag agcaactgtg gcagcaaatg
     4681 ttccaatagc tataacagtt tttgggttta ataatttcat gttttcaact ccctattaaa
     4741 tgattaaata ttttgtttac attcataata taccacggta taccgaggta gtcaacggta
     4801 taccgtggaa attttgaaat aaaaaagccg ccctatgaag agtgtaagga tggctgttta
     4861 tatgctatga agaaatggat tcggcgtatt tggttagttt ggcagcatta tcaaatcgca
     4921 ttttttcaat cgcacttttt ttctttatta aatccgcaat tgttgattct gcgactgaag
     4981 aatgtttgct aatttcatat gctgtcttgc cagtttcaaa tagtaaccat tcgattttct
     5041 ctttatcagc gtacattgca atgctccctc ttgatttttt ctttattata gctctttttt
     5101 gtaataaatc acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat
     5161 ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttaat atactaaaat
     5221 aaaacaacga aagaaggaat agaatgaaaa agttaataat ggtatttact tcgttgattg
     5281 ccatgattgt tattttaggt ggatgtggta gtgaaaaaaa taagcaagat agtcaagata
     5341 atggcgtgtt aaaaccagga acagtgtgga aagaagaagt aggcggactt gttcataatt
     5401 taaaaattat agatgataca acatgggaat attccgaaag tgtttggcat cctgaacaag
     5461 tacaaataac agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg
     5521 attcaggtgg agtagattta ttcataggta aaacagcctt aatagttccc tataacaaag
     5581 acggtttaga aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg
     5641 aaatattaaa agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat
     5701 tgctagtctt tttatttatc ctgaaggagg aatcgcagtg gcaaaagcta aaacgccatt
     5761 tgaaaaagcc gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac agtggaaaca
     5821 agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg
     5881 atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa
     5941 ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta
     6001 gtagctgcgg ttgatatttt tagagcatta caaggtgcag caactagttt aaaaaacaat
     6061 ctgatgattt ttgcggctgt agctgccgtt gcctccattg ttcttggggg gattttcttc
     6121 ttctttggcc gaagaggagc tgaaaacggg aagatgcacg taggttatgt attgatgggg
     6181 gtaggagtta tattagctgc cgcctctatt gtggtttcgt tatttggaat gtttggacaa
     6241 accccacagg gactataaaa aataaataga aggagtaaaa agtacgtggc tactttttac
     6301 tcctttttta tgaaaggaga atactcatgt actttacgat ttcaggcaat accaaaaaag
     6361 ataaagagtt aataaaaaaa gcaacgaaga aagtgggaaa gcgtactgcc tttttaccga
     6421 aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta ggacaagtgt
     6481 tagaacaaga gaatcaatct tctgttaaat tgactctcac gttacatgat aaagaaggaa
     6541 aaatagaaaa ggctggcaca gaatacgttt ttggcccact ggattatcaa atttcagaag
     6601 gtttttatcc tacattgatg gatttgatcc gccttgatgt gtttaaggaa acggatgaaa
     6661 ccatgagtgt ggaagagtct tatcgaaacg aaaaactgct acaagtttta gaagaagagt
     6721 tagagagcga ccaaagaaaa gtgagtcaag tgagtgaagt cgaagtaaat gatgaagtac
     6781 catgggaacg accaatcttt ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg
     6841 acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac gatgtaatgg
     6901 gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg gccgaagtag
     6961 aagttgatct agttgaagct aatgaagagc caacatggac tgaaggggat tctcaggaat
     7021 atattgaacc acttatcagt gaagaagtag agaaaaacga aacagagata caaaaggcaa
     7081 ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg aaagaaaaaa
     7141 ataaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc tatcaagcat
     7201 ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta gaacagttag
     7261 agtctgttca atcaacaaat tggcatgagc ttgcacaaga agatttatta gccacttttg
     7321 aagaacgtca acaagaaaca gatcaagaaa taaatgagta tgcgacggaa cagtcatcta
     7381 attgggagag aacaaaagag gaactaaata aagaagaaca acgagtgatt gaagaagaag
     7441 tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtatag
     7501 agaaaattaa taactttact aaaactaatc aagaagcttt atcgaaagaa aaagaaaact
     7561 tgttaaaaga acgtataaaa gaacgaaaag aagagttcta ccgaggttta cgtacagata
     7621 agctaaagat tagcaaagaa ttaaatgaac aactgaataa actttatcaa acaacgactg
     7681 aagttcttat ggaaagacac gggcagatcc agcaagaatt acaagcacaa attccaaaat
     7741 ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa aaacaacgac
     7801 aagaagaaaa agagcgcgaa caattagaac taaaaaaacg tgagcagcat cttgcagaac
     7861 aaaaagtggc gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa gcagctgctc
     7921 gtaaggaacg tcaagaacaa atggatacat tgcgacaagc acagttgaat tatctagcac
     7981 aacctcaacc agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt
     8041 caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta agcttgcttc
     8101 taggtggagg aacagtattt gcttttaatc atatcaatca atcttcacag gcagattctg
     8161 tagccacaac ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa gagtatgaac
     8221 aacaattgga agaattattg aatcgtacca atcagtcaac agcagagtca agtatggaaa
     8281 atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca actgtacact
     8341 caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtatta gagaaacaac
     8401 gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc atttctatcg
     8461 ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt ctcaaaagat
     8521 tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa taccagaagc
     8581 atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt ttcagttaca
     8641 agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt tgaccccaac
     8701 aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag ctgtacttat
     8761 gggattgaaa atggacatgt ggtttcgtcc gctagcagat gggcaaaaag gagataaccg
     8821 attaaaaacg ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat caaagggaag
     8881 ttttcctggt tatggcggaa taccaattac tcattataaa aaatactggt atattcagac
     8941 ggatacagta aatacttgta ttgttggtac ttcccgctca gggaaaggcc agatagaagt
     9001 tttagcaacc attgacaatt tgtcgcgtgc agaaagacaa agttcaatgg ttgttaatga
     9061 tccaaaaaca gaattatatg ttgctagtaa agatgaacta gaagctagag ggtatgatgt
     9121 gtatgccttt aatatcaccg atccactgca atcaatgagt gataatccat tggcactcat
     9181 tgtgaaatac tggaagcgag gagacataga caccgcaaca cagttaacca atacttttac
     9241 gaatacgatt tattatgatg caaatgcttc agacaataaa tactttaacg aaaatgcgca
     9301 gaaagctgtc aatgcgctta tttttgcgtt attggaacaa gcggatcaaa caggggatta
     9361 ctccaaatta acaccaaata atctcgttga actactttca gaaattggcg gttttaatta
     9421 ttatccagat cctgagaatg aattcaagca gctcaatgcc ttagatgagt tcatgaatca
     9481 gttaccccct ggaaatatag caaaaaaaca atatggggca acaaaaattg gatctgataa
     9541 agcgaaaggg aatatcttgt ctacggcaat tacaggattg aatccattta ccctaacgaa
     9601 aattgcgaag atgacttctc aatcgaccat tgattacaag tccattggtt ttccgaagta
     9661 tttggatctc aaattacatg aaagcctatt aaatcaacgc attgtcgtgg aattttatca
     9721 taagggaaag aaatttcacg aggaacgagt caaagttggc tatcgtggtt tttgtgaaat
     9781 caactttgat tgtcatttag agcagggaga tcatgtttgc ttaaaataca atcatcaagg
     9841 aaaacaatat gttgcttggt atgagttttc tcaaaaagta ttgaaagacg aagagggaaa
     9901 aatcgtttat cgaaaaaaac gaggagaaac tcatttgcca gaactagaaa aagaagccgt
     9961 tctaacatta gatgagaaga aaagtaacct attagtagag tctattcgga tgcactatac
    10021 cgataaacca atggctattt ttatgttaac gcctgattat gatccatcta accatatcat
    10081 tgttagcatc tttcttagtc agttgtataa agaattatct acgcaatgtg ttaagactaa
    10141 aggagataaa tgtcaccgta ggattcattt tattttagat gaatttggaa atatgccacc
    10201 gatggacaac atggaaggaa tcatgacggt tacggcaggt cgtaatatgt tatgggatct
    10261 gtttattcaa tcttatcagc aactttatgc gaagtatgga gaacaaaacg cagcgattat
    10321 caaagaaaat tgtcagacac atatttatat tatgtcgaca gatgataata ccattgaaga
    10381 attttctaaa aaagtgggga ataagacggt tgagcaagaa aacagtacca tcaattcatt
    10441 aggtatgaat actcatatca atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag
    10501 aatttctact tttttagaag gtgaaacgat tgtactcgct cctttaaaac ggcaagattt
    10561 gaaaggtcat aaaatacggc cgtatcctat tttcaatcat ggggaaacga atcttcctta
    10621 tgcttatcaa tatattgaag atttcaatcc atcgtgggat atcaatgata tagacataca
    10681 ttgtacacat gcggacttgg atcttaaggc tttacaaatt gattatgatt atttcctacc
    10741 agatgaatat gctagaaatc tctatcatgg actgcatgat caattagaag aaaaaaacga
    10801 agaaaatatg ttttataaaa tcgttcaatc tcttgagaat gagacggcaa aaaaattgtt
    10861 aattcaattt tacgaagaac aagcagcaga tcgacttcaa gaatatgttg aaaagttgat
    10921 caaaaatgaa gaaatttctg aaatgattgg gaaacggttg ttattggaat ctgaaaaggt
    10981 ggtgaaagaa tgatcattgg aggaattttt tctggcatag ccgactggtg gtcaggctta
    11041 ttcggagaag gggatgcaaa ccccaaaacc gtaacgaaag tcttacaaga ttatggtaat
    11101 tatctgcagt atcaagatgt gttcagttgg atttgtaata cttttttatg ggggttgatt
    11161 aaaggattat ataatctcaa tactttacta gaaaaaacca tttatcaaag ctttgattta
    11221 aaagacttat tgaacgccgc tggggtaaat gaactgtttc aaagtttgat tagtaaggta
    11281 ttggctcttt tttgtgtcgt cacattaatt tatttaggct tgaaattttt tatgagtaaa
    11341 cacccaccaa aagtcaaaaa tatcttggtt aatctattta tggccatgat cttgattttt
    11401 ggtggtagtt ctttaatcga tgaagggtta aatatcagta aaagctttta cggagacgtc
    11461 actgcagcaa ataaagcaga taaaacgggt tcacctgctt ttcaaattat tcaaaataat
    11521 gtgatcgatg tcagtacgtt actagcaact gacccgaata aagtagaatc cttaccaaca
    11581 gataaaagaa atgctttaac agcgaaaaat ttcaaaacag cgaatatcaa tgggattatt
    11641 acacctgatc aagctaagaa aatgtctgaa tcaagtgata ttagtacagt acaaaaagat
    11701 cggttaaagg cattacagta tcgactagag ttagacgata atgggaaaga aatccctgtt
    11761 caaattagtg atgaaggttt agcaaaatat gtgtatacgt caggctatcg tcgatacatt
    11821 agtagtccag ggattatttt agctggtgaa tcttctttgg cggtggccta tttatttatc
    11881 ctatttacga tcgttacttg cattattgag ctggtattta aaaaattcta tttagtgatt
    11941 gccgcgtcaa cggactttga gacaggccaa cggatgaaaa gtgcgataga agatatttct
    12001 caatccttct tgttattggc gtttacgatt ttagaattac gaatctatac attaatgctt
    12061 tcaggtatcg gtgatttgca tgcggcagga aagatcaatg gattcttgta tgttgttgct
    12121 ttaattgtgc taactattgc gctattcaaa ggctctcaat cggttacgaa gattttcggt
    12181 gttgatacga gtctgaaaaa cggcagtaat tctcttctta gcatgtttgc cttaggaaat
    12241 attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta
    12301 agccatttaa atgatattcg taaaaatgga tttggccatc gaaaagatgt tactcctgat
    12361 aacggggaag aacaaccaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt
    12421 tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac
    12481 gtcaatgaac gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc
    12541 ttagaagaga aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca
    12601 tccgaagcac tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg actaaaagat
    12661 gcgtatggtg aaggacaagt taatgcagca gtaaaagcta atacaacaac cgaaaaagaa
    12721 aatcaagaag gtatagtgat tccttcagtt aaagatcacc aagaagcgtt gcactctaat
    12781 gaaaattcga ttgaagagaa aaattccata gaagaaagaa attcagaaga aggaaaaaat
    12841 ggacagttac caaaaactga tccagaacaa tatcggtata gatctgttaa ctccgaaaat
    12901 gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc
    12961 atcgttcttc caaatgataa tggaatcgaa gttggaactg aaaaatcaac gaaaattggt
    13021 ttacctaagg cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggagaat
    13081 gatcaaagtt ttgttcatca atcacctgca ggtactgttc aaatgaaacc aagtcgtttg
    13141 cctgaaaatg gaaatctgac aactgtttct tacgatttat ctgaaagtgg ggagacaggg
    13201 agtgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac cagaacctat tcaaaaagtt
    13261 cacacaggtt acaagccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca
    13321 agagaaacta aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag
    13381 gatatcaaac cataaaaggc ctacaagtta ggcctttttt ctatgtatga aaaggagaaa
    13441 tcatttatgg ataaatatat taacatgaaa aatcctccaa gatttagtgg ggaatggaag
    13501 tttttcgcct ttttcttttt agatctatta acgcttggtg gttgcttttt ttgggcgaaa
    13561 atagtcaata atcttgtcca cttaccactc ttttttgcac tcttgaatta tggcttatgg
    13621 attgcgatag ctctagtgat tgtttggcga ccctataata acccaggtca acgacaaatt
    13681 tttgcgatta tcgaatctat tttctttacg gataagaacc actatcatgc gttagatccc
    13741 aaccgaaatt atcatggtac gtataaggag gatgaataaa tgcgagtcaa aggacaaaat
    13801 tcagttgata agaaatctat tttgcagttt ggaaaaccag aagagcgctc tatcttggac
    13861 ttatttccgt ataaatcctg tacagaagag ggagtcttaa ttactagaga agatcatttt
    13921 caacgttatt atcgtgtcgt ttccactgac gtggagggat taaatgaaca agagaaactg
    13981 gaacgaatga atcagttgac caccgtcatg cgtacgtatg tgccaacaat taagttagtt
    14041 agtttgacca cagagacgga tctaacggaa caaatcgtgc aaaagcggca attattagat
    14101 aaaaatcgta tggaacagtc cttaggaaga aatctacaac gattaaaaaa atatgaaaag
    14161 aaattagtcg aagaaatcca ggaattgaaa cgagcagaac aagaacgtcc tgacttaagc
    14221 ttctttttcg tcattgaagg aaagacaatc aaggatttac aaaccagaaa ccgtcagtta
    14281 atgcgatcta gtggcatttt agggttaaaa ccactctaca aaaaagaatt gatcaagatt
    14341 ctgtatcgaa tgaacaatat gaatgatgag taggaatgga gaagagagaa tgaaatatcc
    14401 gttgatatgg ttagaaaaag atgttccgaa ggaagagatc actagaaaat ttcctcacac
    14461 tttaagcctg ggtaagttgg gaaaagggca atcttttaac caagtagaga atcaaaaaag
    14521 aagaggtgat tgtacatgtt agaagcgatt aaaacagctt tttcaccaca aaatgatcaa
    14581 aaagtacttg atccattaaa gcaactagga aatcgggaaa tagaaatttt aaaatccaaa
    14641 gggtatgatt tagattttgt aaaacaagtg atgccacaag gtggattaaa tttcgatgaa
    14701 gagtatgtga cctatggaaa tggttattca cggtgtgtga cagtttatca ctacacaaaa
    14761 aatcctactc ttttttggtt ggccaatctc atgaacaatc ctcatacagt ggccacactc
    14821 gatgttagca cagatgaaaa gcaaaaagtg attgatcaaa tcaatcgcgc attagatgaa
    14881 ttaaatgatc gatcagataa tgaacgaaaa gcgactgacc gaaataaaag cgcggaagaa
    14941 tacctggaac tagcggaata tgcccgaaaa ttgacttcta atggggaaat ttctaaacaa
    15001 gtcaaagtga gagtacatgt ttacggaaac acgattcaag aagtagatga acgagcgcag
    15061 gaactgatcg aaaatctcaa agggatggat tatcgtgcgg tgacttatct gcataaaacc
    15121 aaacgagatt ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg gttcggaaat
    15181 atcaaagggc aatcactacc atcgttaaac attggcggag gtttcccgtt ccaccaccaa
    15241 acgctaaaag atccaggagg tacacaatat ggggaaacca tgacaggtgg cccatttgtc
    15301 tggaatgcca cgtataaaaa tagtgtacgg ttgtctacta ataccttgct tctaggaatg
    15361 atgggagctg gtaaatcgac cgttcttaaa atgatcgcgg aatcccattt agctgcagga
    15421 gactttattt ggggctttga aaagggaaaa gactttattc ctttcttaaa ggaatataac
    15481 ggtattatgg tgcgtttaga cggatctgac ggaatgatca atcctttaga gatatttgcc
    15541 acacggacgt atgatgaagc atccagttta tatgacgatg gatcagtcaa agatttgaag
    15601 atcaatgaag cagcgtccta tcaaacacac ttagacaaag tagtctatca ggtacagtta
    15661 gtgagtcctc agctaaaagg aacgatgaaa gcagaattta agacctactt aaatcgtttt
    15721 tatgaagagt atggaacagt accacgcggc tttacttcct caaattcgcg ttcaaatacg
    15781 gagacgcaag tcacagggaa agatccagaa gcttatccga cgttcaaaga atttttagac
    15841 tttttaggac aactggaatt accaggtgct tcccaagaga aaaagaatcg aaaagaagaa
    15901 atggaatcca tcgtagaatc cttatgtgag acgtatggca tgatttttga tggtcacagt
    15961 acgattcgac accttgacca acaacagttg gtcttttttg acattgactc tattacaggc
    16021 ttagatcgag gggtacaaca atgtcaaatg tacatggcta tgacgttgat ttggaatcag
    16081 gcattgattc aaggaagaag acaacgacgt ttacttaaag aaaaacaaat ggaagaacaa
    16141 gacttcaaac gcttcttggc gatgattgat gagtgccata atggtgcgac ctgttctgca
    16201 gtgaaaagct gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa
    16261 gtcaaatgag tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta
    16321 gtttatgcag accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt
    16381 aaaagaaata gtgaatacca cgaaagttct ctagaggtaa agggtgtatc cgataagtcg
    16441 ggtgtccaaa aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg
    16501 tactgtccga aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat
    16561 tgtgctaact tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc
    16621 gtaggagaag ttagggggca aaagctagca tctaagttaa ggcatgtgga tagatataag
    16681 ggaacagggg aagccatcaa catggagagt gcatactcga tgaagtggta gtaagaaata
    16741 tgtgaatgac aaataactta ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt
    16801 tctgtaatgg aactggagga acaggctcga ttcgtctgta aaagtcatga aacccgttca
    16861 accatgggat tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg
    16921 acaaaagaaa caagtccaaa agataggaag ttgcggcatg cagaatatta tggcatgacg
    16981 gaaatcttcg ataagatata ctccaaaagt ttgaagggag ataatttcaa gaatctaatg
    17041 cagattatca tttctgataa caacattgtc ctagcatatc gtaatatcaa gagaaatcac
    17101 ggtagtgtga caagaggaga agatggagta acgattaaag atattgaaaa tctcaaccgt
    17161 aatgaattcg tagaaattgt taggaagcga ttcgcacatt ataaccctcg gaaagtaagg
    17221 cgtgtagaaa ttcctaaata taatggtaaa ttaagaccgt tgggtattcc atcaatatgg
    17281 gatagaatcg cgcagcaatg catcttacaa gtattagaac caatatgtga agcaaaattc
    17341 aataatcaca gctatggttt ccgcccaaat agatcagcag aacacgcaat agcagattgt
    17401 atggcccgaa tgaatcaaag tcacatggaa tatgtggtag atgtagatat caaaggtttc
    17461 ttcgacgaag tcaatcatac taaactcatg agacaaatct ggtctatggg tatacgagac
    17521 aaacagcttc tcttaatcat taggaaaatg ttaagagcac ccgtagtact tccaaatggg
    17581 aagttgatat acccaacaaa aggaacacca caaggcggaa tacttagccc attacttgcg
    17641 aacatcaatc tcaacgaatt tgactggtgg atagcaaatc agtgggagga aaaagatgcc
    17701 aaagagctaa aacatcattt taggaaaaat ggagcttggg ataaaggtaa tatctatcac
    17761 aagctcagaa agagtacaac tctaaaagaa atatacctag tgagatacgc agatgatttt
    17821 aaaattttct gtagaaatta taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg
    17881 ttacaggagc gtctaaagtt accaatatct gaagaaaagt caaaaatcac aaatctttca
    17941 aaagaagaaa gtgaattttt aggctttacg atgaaattag tgaccaaagg taacaaaaag
    18001 gttgttaata cgcatatagg tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa
    18061 caaatcaaac gaattcaaaa aagtcctaat agcaaagata cctttatcta tattggagtc
    18121 tataacagta tggttattgg gattcataat tacttcaaaa ttgcgactca ttgtagtgta
    18181 gcaatgcgaa aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa
    18241 ggaatctcaa aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca
    18301 ggacaaatac gatatataag agggtttcca atattaccag tagggtatat tcaacacaga
    18361 catccgatgt gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt
    18421 cataagaagc aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg
    18481 cttagtaaga gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa
    18541 aagggaagat gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat
    18601 attatccctt atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag
    18661 gaggttcatc gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat
    18721 ctaaaattaa atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac
    18781 gaagaaatat cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag
    18841 ggaacgccgt atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat
    18901 aatatcaaac tcttacctat cagtatcgat taatccagat tttattgaaa cggtaaatta
    18961 tgtcacaaac tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca
    19021 atccccacaa gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa
    19081 agtctttgaa ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca
    19141 tatagaagga ttagttggta aatcaatgac gaactcagaa ctagaatcca ttcctcaaca
    19201 aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag
    19261 tccgaacaaa cgtcaattga aattgtttga tggtggaaag taacttcttg tattttggtg
    19321 tatacaagta tatactaatg tgtgagagaa ggagggattc catgttagta aaagaaatcg
    19381 aaatgaaaaa atggggaaat tcacaaggta ttcgttttgg aaaagaagaa ttgtcagttc
    19441 taggagaaac taatactgaa aatttaaaat tcgaaatggt ggtaacagaa ggaaaaattg
    19501 ttttaactcc taaaaataaa aagccagaaa ctttagatga attgttcgcc aattatgaag
    19561 gagaaccctt aggaaaagaa gacaaatatg aatggggaaa tatgcaaggt agggagatca
    19621 tttaatgaaa aagaactatc atgatttatc gcaaggagat cttgtttata ttgatttaaa
    19681 tcctaccaaa ggacatgaac aacggggaaa acgaccatgt attattttat ctaaacctaa
    19741 taaatatttg aactatatga ttggtgtcgc accgataaca acaaaagcga agccttttcc
    19801 tgtacatgtt gatttgaatg gtacgcaaaa aataaaagga caaattcttt tggaacacca
    19861 tcgaatgatt gacattgaga gtagaggttt tcagtttgtg gaagctgtgc caaaggaaat
    19921 tttagaagaa tgtattgaaa aaattaaatt attttattaa aagcagcgaa gaataattat
    19981 tatttcttcg cttttttgtt gtttaaaatt aaaaagttag gagtactgac tatatggaaa
    20041 atgatacaaa tatagttatg gaacatttga agcgtcgtgc aaagcgacgc ttatttttgt
    20101 ggttgtttgg tacttctgca ggtttgatta ctattttgat cactgtgttc gtaacccttt
    20161 ttctaatact tgcggcgggt tcgattgata attcggattc cgattcaagt agtggtggcg
    20221 aagcttttac aggagagtat tcggaaggct taccgattta taaagaaatc aaaggaagag
    20281 gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc agcagtgaaa tataaattat
    20341 taccaagtgt gattctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg
    20401 caagaaatga tctcaactat tttgggatta cttggtttga tggttgtctg tttcctaaag
    20461 gaactgcacg aggaattggt ggcattgaag ggggctggta catgaagttt cccaactcaa
    20521 aagctgcatt ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcatgtg
    20581 taggaaataa aagccctggc gcaagcttat tgatcttagg acgaggtggt tatgccgctg
    20641 caggaattac tgaagatagt ccgtattata caggttgtat gtcaattatc acttctaata
    20701 aattaaccga atatgatgaa ttcgccatca aacattgggg tgaaggtggc aataacaatg
    20761 gcacaattac tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagttcct
    20821 ttagcggtgg tcaattattt ggaacgaatc cagggggaga gttccgacca aatggtttcc
    20881 atgacggatt agattttggt tcagtcgatc atccaggaag tgaaattcac gcggtgcatg
    20941 gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgca tgtgtgatcg
    21001 tgattaatta cgacggtttg aatatggtgt atcaagaatt tgcgaatagt acaggaaatt
    21061 ctcgtgtgaa ggttggagat caagtgaaag ttggtcaagt aatcgggata cgagatacag
    21121 cacacttgca tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca
    21181 cagatgatgg tacatggatc gatccattac catttttgaa tagcagcaaa aaatagatca
    21241 ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt aacagctgca attagcgtta
    21301 gtttaatcgg caatatcgcg ttgttttggc agcttaaaga agaacaagag acaaatacct
    21361 ttaaaattga agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg
    21421 cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc
    21481 agctgaagca acaagatggc acaaaagcaa aagaggaagt cactgaagca caaagaaaga
    21541 cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat gaattaacgg
    21601 aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct gcagacgatg
    21661 atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttacg
    21721 ctcaaacaag cagtgtaaaa gaggaacaaa actttgtggt atttgttgat tatacaattg
    21781 gcaatcctca attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg
    21841 aaaaacaggt agatggtcaa tggaaagtca cagactttag ttatttcact cgttaggagg
    21901 aagaaaatga gtaagaaaaa aggagcaaga caacgtttta aaaaacgaaa aagttatctt
    21961 agatggctgg gaatctttat gattctcggc tttttgattc ttgctagtcc gatttacacg
    22021 cataagaagg tagacatacc agatgaagta agcacgaacg cctttttaaa cacgaaggaa
    22081 ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc
    22141 gacgatgaag atcagagctt attaaaagaa ctagctaatc ttaattatcg tgtagaagta
    22201 aagacagcta aaggggatta tcaagcaata aaagccaaag tgatcaaggt gtctgatgat
    22261 tattttgtag tgaagctatc gcatgtacca gaagaatata ttgctatgcg attaaccatt
    22321 atccctgaaa aaattgatcc aaaggtggat atgcaagaac cgcaagatct gatttattac
    22381 gttcacgaag ataaggtcaa agatcacgtg aaagacgaag attatgaaca acatgcgatc
    22441 aattacaaag tgaaaggata caagaaagaa caaaaagcag cacaaaaaca aattgaatct
    22501 ttccaagcaa ccatagattt gaatgaagaa ttagtgaaga aactaaaaga tcagttgcct
    22561 tatcaagttg cagacgatca ggaaaatact gaaaataaga ttaatagtta ccagcaagaa
    22621 attgaaacat cgaaaacgca aatgaaagat caagaagaga tcattcaaaa acttcaagaa
    22681 aaaattgatc gtatgacaaa agaaaatatt tagaagaata ggtggagtaa aagaaatgaa
    22741 gcgagcaaac ttggtcggca aagatacttt cgctgagcag aaagtattga ttaaagccta
    22801 tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg aatttatcta acacaacaat
    22861 atctgagttg aagaatggta gaaagagttt agatacctta aaattcagat catttgaagc
    22921 tctatatata gctgcaaaaa aatattttac ggctgaaaga aaataaaaac aggaggattt
    22981 tatgtgaaaa aagtatatca aaaatgttta tggatcagct atatcaccgt aggtatagct
    23041 gtttttttct ttagtttagc cgttatcgtc ggtacaattt ttctttggtt tatcaataaa
    23101 gatttatcat ttaattggtt catgagtcta ttgtcgttca ttatgttgat ctttttatgt
    23161 ttagaaatcc gcgaattcat gccttctcat tggcaagata agaggaaaga gtttagagaa
    23221 gaaatcgaaa tgaaatcgag cgactggatg gaacatactt ggttaccatt gaaaagaaat
    23281 agcaaacgaa aagaattttg gattctacaa ttatggccat taggtaaact ttgtagctat
    23341 ttggctcgtt ttatacttga tttaatcatt tactcgattg tagtatttgg aacggcttat
    23401 tacttattaa atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg
    23461 ttgatttttg tcatgctatt tactattttc actcaaatag ccatgcaatt taccaggcaa
    23521 aaattaacag caggaaaact tttcaaaagt ctattcgatt taattattgt aaatgcaatt
    23581 ctagcaactt taataaaaca aatcgaacaa tttggaagaa caacaaaagg actgaaggaa
    23641 tttttaatga cgtttgttca ctttgtaaat gggtacatgt atttgtttga agtgggtatt
    23701 attctctttt tattcgggta catatccacg aaacttatta aacgttcaat tgaaaaagaa
    23761 attgagattg acctgattcc gacacttttt ggttatgagt actatgttga ttttggaaga
    23821 tatgacgaat tgatcaatac aggtattgtt cacacgagta tctatgaagc gaccgttaag
    23881 ggcatcagaa ctacgttaaa agatggccag gagtcgaaag caattgaccg aagaaaagag
    23941 gtctacaatt tttatgaagt cgaagcaagt ctaagatatg gtattggaaa attgtcgtta
    24001 gatggaagaa atattccaca aaacaaacca tacattggct ggaaaagtga tgctttagaa
    24061 gtatctaaaa agctttctat ggaagaagat gagcttcaat gagttcgatc aatttgaacc
    24121 atttatttga agatatcagt aatcattttc aacaattttc aattgaagat aatgatgaat
    24181 tgatgggatt attccaaata gaaaaagaat acgactattt gacatttatt tgtcgtgaag
    24241 gaagaatcat tgatcccaaa gaatcaaaaa tcatttccca tttaagacga ttcaaaaatt
    24301 atatcagtaa aaataaagaa actatttcag aaataaaatt tgacggaaca acaaacttag
    24361 aatatttagt aagagtcacc tatcaaagaa accgccgact gattgaagat gatcagggag
    24421 ttttaatatg tgctggtgaa gaatacgata gtctcaaatt tactcagtta aaatttgaaa
    24481 gtattggtcg ttctatttat tttgagtcac gaccatttta tatccgatat aatgattttc
    24541 aacatttgtt tttacaagat gaaccaaatg gattaaccat tctagaacga agagggaaga
    24601 aacaaattac attatcctac gagagacccc tcacattcat ggatggttca tgtgaatcta
    24661 tttttcaaaa aatcaatccg tttattaaag aaatgatgaa agaaaaagat tcattacctt
    24721 tcacttgggc cgagattata gaagcaaaaa ataaatatga attattgtgt aagaagtatc
    24781 cgtcaaaaaa atttactaag aaggtaaata aatatcctct acgtcatact tatgcgctga
    24841 ttaagttaaa accaagagta accagaaaac agtttagaag aatagaagct gcgatcgaac
    24901 aggaagacat tgatgttttt ccttcagaaa gatttgattt tataaggaag aacagccgtg
    24961 aattcgtaaa atctttatta attagttatg ttcaaaactg tattttacca agttttgcaa
    25021 caattattct atctgattta atggacatga gtttttattt aaatcagaaa ctcgaattta
    25081 attttaaaac agaacgagga ttattaagac agcataatca aattgttgaa gaatataatt
    25141 tgaaacaagc taaaagatta aagacgttca aattgaaaca acatggtaaa tacagattgc
    25201 ttgtcaaaca tttacaaaat catcctcact ttaatttgat taaaacaaat aaagaactct
    25261 ttatggaagg atcaactatg caccattgtg tatataatta cttagaaaaa atacaaagag
    25321 gtggttcaac tatttggaaa tatgaacgac agaagcatcg ttatacagtt gaaatagaga
    25381 aaagaaagta tggcaattat gaagtggtac agtgctatgg aaaatacgat tcattgccag
    25441 ataaacaaga acttgatgag atcataaagg ttgttagagc tattccgtac aaatgatctc
    25501 acctcaagtt cttcttaatt tttaaagagc ataaggaaac gtgaccccac ttttaaaaaa
    25561 agtggttgga taggctgtgc ctatcacgtt tctgaaaaaa ataaatgacc ccaaaatacc
    25621 aatatatagg atagtacaag gatagcaaag gatagtaaaa ggatagaggt aggatagtga
    25681 aaaggatagg ctccttttca ctatcctata ttatattgga ggatatttat gaatgttaaa
    25741 attagagatt tagatccaaa attcatttct gaaattgatc gaagatgcgt tgaactaagt
    25801 aatcgtacag gaaataaatg gagtcgtaat gattatttaa agctactcgt tgaaaatgat
    25861 tttgatcgtc ctttgatgga atataaacaa gataaatttg atcaattatt aaatcaatta
    25921 atagataaat tttcttttgt tcaaagtcat aatacaaaag tattagaaga atatatttgt
    25981 cagaataatc aaattatcga acttttaatt gaacaaagta gaggaggaga agtttaacat
    26041 ggccaaagtt atagacaagc atatttattt ttatgaaaca catatagatg ttctagatga
    26101 aattattgca actcatgcag aggttaaaaa ctatagtgaa gccgttcggt ttctatgtat
    26161 gaattatgat ccagaagtgg agaaaaaagt aaattccaat caagcaaagt tgaacggaat
    26221 gagtaaagaa atttctattt taaccgaaat ggtttcagag tttgcggatc tccatttgaa
    26281 agatacaggg attttaattg gtacaaattc aactgtttac caagaagcaa aagagcgtgt
    26341 ggaaggcaag ataaaagcaa atcaaacgaa gaaatattct cctaagataa agaaaacaat
    26401 aagcgaatca gtgactacaa aaagtgaagt atttgatcca acaaggttct taaaacgata
    26461 aaaattagaa ggagaatgtg atgagtaaat cacctgcagt cattctcact tgtcaattta
    26521 ccttgcccaa taaaaaatct ttttcaacat acattgatta tatgacaaga gaaaaagcgc
    26581 tggaagaaaa ggaacgacgt acgcccgagg aagaacagga actgcgtcta atcaagaatg
    26641 ctttagagga tttttatatt cctgaaggag aaactttttc ggagagtaag acggataaag
    26701 aattcagcga aacgtcgaat gaagctcaat ctttgatggg aaatggaatc aactttgaca
    26761 agttagagga acaagatttt accaaatatc tttcttatat gacccgtcac tatgcgttag
    26821 aacaaaagaa agaactgacg caaagtgaac aaaaagaata ccaacgcttg aaccaagcaa
    26881 taaaaaaata tcaaacagag ccacacaaaa aagcttcagg aaaccttcct ggagtttttt
    26941 ctatggcttc agacgaagtg aaaggaagtg atctgaagga gattaagcaa atcttccaaa
    27001 gaggacaaca aaaaggctca atcatttatc aagatgttgt ctctcatgat aattcgtatc
    27061 ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagaa ggattaaaag
    27121 cagctggaag aaaaatgatg gagacattat tcgagaaaga gcaactgaat gaaacaggct
    27181 actggatggc aacgattcac cgaaatacca aacatatcca tattcatttt gcagttgttg
    27241 aaaaaaaaaa tacgcgtaaa ccatacactg agattgaaaa tggtgtgagt tatatcgtac
    27301 cgaaagggaa gagaaggcaa gcaacattag acgaaatgaa gactaattat gtacatgagt
    27361 tagaacgttt ttcttatgaa aaaaatcgaa tgggtatgga gaaacgaaat ttattgacac
    27421 ggaaaagtga tttgcgtaac accttaacca aagtagtgaa agagccagta cgttatgatc
    27481 gacacgcaat gagcttatta aatgaagtat ataaaaactt acctaaaaag cgttcggaat
    27541 ggcattacgg ggatgaaaat cgaaccaaac tttcaccata cacaagagag aaactcgatg
    27601 agttaaccca atatgtgtta acaaaagaac ctggatatca agaatatata cagatttcaa
    27661 aagcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa tcaaaggatg
    27721 cagaaaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatt ctaacagaac
    27781 tgaagaaaca ggatagtcaa ataaaacctt taatagaaag gatggatcag atgcaatcta
    27841 tttacaatga acgatccttt cgaacactta aacagttgcc aacgaatgaa tacaaatata
    27901 gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc acgcaacgga
    27961 gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac gcaagtaaag
    28021 catacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtag gaggaatagc
    28081 aatgaaacaa tatttagatc aatggaaagt tatagaagga tcattaagag aagaacgaat
    28141 cgaacaactt cccgattgtt tataaaaaga gcatttgttt cagataagag aaatgttgag
    28201 aaacgaacag tttgatccaa atcaattttt agtcgttgag tacccagcta ctggtgtgta
    28261 atgttgtaat catgtgaagg gtgaaaaata ttttatcatt caaaaatatg agggcaagtt
    28321 agcgccttat tatactactt gggaaatgaa tgaagagggg atcaataact tcccgtgtaa
    28381 aagcattgaa gagtctattt cactcacgga atgttaaata aatagagaaa taaaagagtc
    28441 ctgaacttga ttgtccaggg ctctttttaa gtgaattttt ttccgtcaat tcactgagtg
    28501 gtgcgattca attttattat atcaaaagaa agtgagattc caaagagaag ggggggaaga
    28561 agacatgact cagacaacga ttggagttgt tgttgaaaaa agcagagaca atctagtgtt
    28621 tgtgacagaa gtcgaatcag aaagaacatt tgttgtcaca gacaaagaag caaaagcgta
    28681 tcaaccaggg gatctcttaa cactagatct cactacaaaa aaatttgtcg atgctgcaga
    28741 aaattatcct tttatttaag aaaagatggt gaacaaaatg gaacaatttc aacaagtaaa
    28801 tcctgaagta caagaacaac tgaagaaatg ggaatcactt tcacgtgaag cacaaaatga
    28861 aattatagtg atcttttctc aaaatcctgt taatttcaga attgctttaa tgaaatatga
    28921 tttaaaggat actgaaaata tttttacagg attgggtacg cctataggga aggcttggga
    28981 agaacgatac aaagagttag aaaaagaagt tagtaccttc aaagcaaccc gtagagaatg
    29041 gttagatcaa tatgaagata gtggggttat gtatattcca gaaagtttat gggagacagc
    29101 tgaagagtta ggaataatga aaaactaccc caaagtagtt ccaactagcg aactcactgc
    29161 accagttttg ccagaagagg taaagggaaa aagtagtctc actttaaacg aagaagaaat
    29221 cactggaaat agtcggcttg cacaagcaca aagaaagctt atccgattaa gaaatgagta
    29281 tgacgcgaca tttgaaaggc tggctgaaca ccagaaatta acaaatggtc agccaatgaa
    29341 tgataaaaga aacggatcta gctggtttag tcaaagagaa aaaatcgaaa ataaaattaa
    29401 tcaattgcaa gaagagattc aaaaacaaga aaattatgtt gagaaattga ctagacaatc
    29461 tgagcttaga aaaatgggat ataacaaata tggtgggctc gatcttacaa tcgagaatat
    29521 tccacgaatc aaagaggaga tcgaaaaatt taggaatgga aaatctcaat tttcagtaaa
    29581 aacaatcgaa aagtatcaaa aaaaattagt tgaattggaa cagttgaaag aaaaatcaga
    29641 gaaaggaata gccaatattt tgcctgaagc gcaggcaatt attgaaagtg gccgtgttag
    29701 tcagtggaaa agaaatccaa ctatatactt tgttaaaggg tatcgtaaag tggcattaga
    29761 gttgaatgaa caaggcgaat ttgaagtatc aaaaagatac caaccaaaga ctgaagaaga
    29821 aaaaagaatt gtgagtgagt tacttgaaaa taagatggac gaatatggtg ttcaaaagca
    29881 agaacccaat cattattatc gtgtagaatt caatgaaaat gatggaacaa tgcggaatta
    29941 tcaaggtgaa atcgtgacac aatcactact tgaacaaatc aaagcattag acacacacta
    30001 tatagggaaa acaagctacc acaaatttta ttttgaagaa gtaaaagaag gtgaagttgt
    30061 taatcagtta cgactagacg ttggagacgg gttacaattg aatgcttcta tttatcaaaa
    30121 gctagaggag ttatgtgacc aaacattaga aacgaaaact gaaaaagtaa taaaaaatga
    30181 aataggtagc cacgataaaa aggtagagac aaagaaacca agttatcaaa aacggtcaac
    30241 agaagagatt aagcaagaag tcaaattgct ttcccaacag gcactagatg cggtgaaaaa
    30301 atatacccat tctcctgaag acgtcaaaga actcttgaac tttatgtcgc gttttccaga
    30361 acgatctttt agaaatcagt tattgatcga aaaacaattt ccaggagcaa cggcgtgttt
    30421 aggtcgtgct gcgttgaaaa aagaaggcat ttatatcaaa aaaggggaac aagggaacaa
    30481 gatctttgtc agaaaaacgg tcaaaggatt ctatgataaa ggacggggct ttgttcgaga
    30541 aacagaggct acaccagaag ataaaaaacg aatcgaaagt ggccaaatcg aagtgatcaa
    30601 aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacagt tgaaacctga
    30661 agactatccg aaaatcttcc cgaaccgtgt gtttgatttt caattggata aaaaaggaca
    30721 agcagaacta caagctggaa ttcaagcggt agccaacact attgggatac aaattcgaga
    30781 tatgacagag agtgaagtct atcaaagaga attaggacaa gcacgtgggg cgtatgttca
    30841 taacgaattt actggtcaag aagaaattgt catgaatact agaaatacac cgacgcagca
    30901 tttagctaca agtattcacg aattagcaca tgccagaatg cacaaatttt cagaattaga
    30961 cactgcgacc aaagaactgc aggcagaaat gacttcttat attgtgtgca aacattttgg
    31021 aatggacacg tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac
    31081 gttggatgat aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc
    31141 aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga
    31201 gatccaacca gcagaagtga agcaggagca taaaaatact ttagaaacgc cagagaataa
    31261 gaatattgtg cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga
    31321 agatacgggt cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa
    31381 tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga
    31441 agaatttgcg ctaggaaatc cattccgatt ttcacttgag acagtgtcag aagtggccaa
    31501 acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa
    31561 gcaagaaaga ctatctgaaa aatggatagt cttttttctt ttgggaggtt tgaaaatgac
    31621 agagaaaaaa atgagtctta ttgatcgatg caagcaaatg gatattgtag attttgctcg
    31681 aaacaatggt ttagcggtag tcaataaagg acgagactat cgtttagaag accatgattc
    31741 ttttgttttc gatcgaaaaa aacagcgatt ttattggaac agtcaaggga ttggtggaga
    31801 tattattgaa ttgactcaat tgttttttat tgacgattct attcaaaacc caaagcaacg
    31861 ttttaaagcg gcgctagatt atattttaaa ggacgaaagc aaggtagaac gggtggaaaa
    31921 tcttcatttt gaaacacaag aatacaagca ccatccaatg gattatcaac cactcacaga
    31981 aaaaggacga acttacttaa aagaacaacg aaaatttcct gaatggttga ttgaatatgg
    32041 agatcaacaa gggttgatct tggaaatgaa gccacgaagt gaacgaaaaa actatattgt
    32101 gaaagatgat cgtttagact acgcgattgt ttttccttgg aaagaccgac atacagatca
    32161 agtattagga gcaacttatc aaggaacgat tattgattac gaacgttttg gtgatcgggg
    32221 aacgtacaaa catatcgata tcaatcctac tcctaatcac ggatttaatg tccttcttgg
    32281 ttctcctaaa aatttaaaat tttttgagag tcctattgat ttattgagtt acgttgcttt
    32341 aaatcgtgag acagtcaaag atacatggct catttctatg gacggactga aggatgcagt
    32401 agtaggacac tatgttcgag aagcggtaac ggaattagcg ccgaaagagg aatttccaga
    32461 atcgatcgag atttgtgtgg ataatgatcg agctggacac atattttggg ataaggtaca
    32521 ccgactggga gtgtcgaatc cttttacagg aaaagcggtg tattttgaac ctgatcttcc
    32581 caatgattgg caagtaccga gaaaatacca aactatctac gaagaagtag ggaaagaaat
    32641 gaatgttgca ccagaagcga ttatggctat tcataaaatc gaaaacaatc taacggagaa
    32701 taaccagatt gtattattcg gtgaaattca tggagtattt gccaaaaagc ttgcgtcaaa
    32761 agagaaagtt cagatcattg atgtgaaaga gaaatgtata gaagccgctc agcagttaaa
    32821 atcgtgtgaa aaatctgatg gaacgtacga ttttgatcgc ttttatcgtg gaaaagggaa
    32881 aattaatgaa gaaattcaaa tttcattgaa ggccaaacat tattttgaag gttataaaaa
    32941 tcatgatcat gaatttgttt ctgaagtgaa gaaggattgg aatgatcaat ggaaacatga
    33001 gatccagcaa caagaaatta gaaagcaaaa acgtgccatg ttgtttcaac aaagcagaca
    33061 acaagaaagg gagtgaaaga agcacgatga aaactgtcat attagcagaa aagccgtcac
    33121 aagcgaaggc ttatgccgat agcttctcta aagccactcg aaaagacggg tattttgaaa
    33181 tccaagatcg attgtttcct ggtgaaactg tgatcacgta tggttttggt catttagtcg
    33241 aattagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta gaacatttgc
    33301 ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag caattcaagg
    33361 tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat agcgatcgtg
    33421 aaggggaact gatcgcttgg acgatcattc aacaagcagg cgcagagcag ggaaagacct
    33481 ttaaaagact ttggatcaac tccttagaaa aagaagctat ttatcaagga tttcagcagt
    33541 taagagacgc aggagaaacc tatccgaaat ttgaagaagc acaagcacgc cagatcgctg
    33601 attggttgat cggcatgaac ggcagcccac tgtatagttt actcttgcag caaaaaggca
    33661 taccaggaag tttttcctta ggcagagtcc aaaccccaac gctttacatg atttatcaat
    33721 tgcaggaaaa gattagaaat ttcaagaaag aaccttattt tgaaggaaaa gccgaagtaa
    33781 tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag
    33841 aagcatttga agattattta aaagaaaagg gggttcaatt gggaaaacag ccaggcatga
    33901 ttcgccaagt tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt tctctatcta
    33961 gccttcagtc taaaatgaat caactcatga aagctagtgc caaagacact ttggaagcca
    34021 tgcaaggctt gtatgaaggg aaatatctaa gctatccgcg aacggatacc ccttatatta
    34081 cggaagggga atatgcgtat ctgttggatc acttagacga atacaagcat tttttgaggg
    34141 ctgaagaaat tccaacccct attcatacgc cgaatagtcg gtatgtgaac gataagaaag
    34201 tccaggaaca ttacgcaatc attcctacaa aaacagttat gacagcagcg gcttttgaac
    34261 aactaagtcc actgcagcag gctatttatg aacaagtgtt aaaaacaacc gtggcgatgt
    34321 ttgcggaaaa atatacctat gaagaaacaa caatcttgac gcaagttcag cagcttcagt
    34381 taaaggcaat cggaaaagta ccgatagatc taggttggaa gaagttattt ggcaaagaga
    34441 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
    34501 tgacggtaga tttacaagtc cttgagaaag aaaccaagcc accacaacct tataccgaag
    34561 gtacgttgat cactgcgatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
    34621 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
    34681 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
    34741 gggttttgct ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg
    34801 ccaaatggga aagctacttg ttaaagattg gagaacgaaa aggaacacaa accacttttc
    34861 tagccaatat ccaaaagttt gtctctcact tattggaagt cgtcccagga caaatccaat
    34921 ccattgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
    34981 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
    35041 tggcagcttg tacgtctgaa gcctgtgatt ttaaatgttg gacaacgatt gcgaaaaaaa
    35101 aactcacagc gacacagctg aaggagatca ttcaaaatgg ccgaacaagc caaccagtga
    35161 aagggttaaa aggtcagaag ggttcgtttg aagcaacgat cgttttaaaa gaagacttca
    35221 ctacaggctt tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca
    35281 ctacaaaata atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg
    35341 aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac
    35401 tgcagcaatt aaagaaccag cagcggattc aaaaagaaat tgaattagat cgatagacat
    35461 taaccttgtt acaaatacac tatcatgata tatttaatgt agtgtatttg taacgaaagg
    35521 aggggaagta aatgcaagat aaactgaaag agttgttggc tattcgcgat ggaaatttat
    35581 ctatgaagga agccagaaaa tttggaatcg cagcaaccac tgtacaaaga ttggtgaatc
    35641 aaggaaaatt gatcaaagtt gatcgtggtt actatgtaga ggatggtcat ggaatagatg
    35701 atttattttg gcttcagcaa cgattttcca ggggaatctt ctctcatgaa acgactttag
    35761 atatttacca gctttctacg aatatgccga aattcattca cttaactttt ccccatggat
    35821 ataacgttga tcgttcaatt acaaaagagc agcagttaca atttcatttc gtgaagaaag
    35881 aagtttacga gctaggaaaa gtggagggaa cttcctttca aggaaatcct atcactatgt
    35941 atgataaaga gcgtacactt tgtgatattt ggaatccgag atatgaaatt gaatacgaaa
    36001 tgaaattaga agcattaaag gagtacatgg gagatccatt gagagatcca agcaaattaa
    36061 gagattatat gacaaaacta catgtagaaa aagaaatgaa ataccatatg caaagcttat
    36121 attgatggag gaaaaagatg acaccgacac aatttgcagc aaaaacaaga aatttagcaa
    36181 agaggacagg attaaatgca cagttagtac aacggcattt tatgatggat cgtttaattg
    36241 aacaaatttc tgaatctaag tataaaaatg atttcatttt gaaaggtggc tttttattag
    36301 gatcaaaata tggaatcgat agaaggacga caattgatat cgacacgaca tttagaaata
    36361 gcaaattaac tgaagaaaaa ttgaagagta tttttaatga tttaactagg aaacccacga
    36421 aagaaggtat tgttttctct attcaaggaa tgaaggaaac aagagaagcc gatttttatc
    36481 ctggatttca aattaaattg gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg
    36541 acgtgacaac aggagattct atttatcctg aagtagatac acactatcac caattaatgt
    36601 ttgaagataa aaaagtggag attccagctt atccaacag
//
LOCUS       DACCVU010000017        35133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCVU010000017 DACCVU010000000
VERSION     DACCVU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.017"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..190
                     /locus_tag="IVW29_000713"
     CDS             <1..190
                     /locus_tag="IVW29_000713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7838213.1"
                     /translation="TSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFS
                     LEVQDLDGNTLEPVWMKM"
     gene            427..969
                     /locus_tag="IVW29_000714"
     CDS             427..969
                     /locus_tag="IVW29_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7838214.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1052..1603)
                     /gene="lepB"
                     /locus_tag="IVW29_000715"
     CDS             complement(1052..1603)
                     /gene="lepB"
                     /locus_tag="IVW29_000715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7838215.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1755..1982)
                     /locus_tag="IVW29_000716"
     CDS             complement(1755..1982)
                     /locus_tag="IVW29_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7838216.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(1979..2497)
                     /gene="msrA"
                     /locus_tag="IVW29_000717"
     CDS             complement(1979..2497)
                     /gene="msrA"
                     /locus_tag="IVW29_000717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7838217.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2517..3230)
                     /locus_tag="IVW29_000718"
     CDS             complement(2517..3230)
                     /locus_tag="IVW29_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7838218.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3233..4129)
                     /locus_tag="IVW29_000719"
     CDS             complement(3233..4129)
                     /locus_tag="IVW29_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7838219.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4287..5129)
                     /locus_tag="IVW29_000720"
     CDS             complement(4287..5129)
                     /locus_tag="IVW29_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7838220.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5261..5914
                     /locus_tag="IVW29_000721"
     CDS             5261..5914
                     /locus_tag="IVW29_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7838221.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(5977..6504)
                     /locus_tag="IVW29_000722"
     CDS             complement(5977..6504)
                     /locus_tag="IVW29_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7838222.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6524..7471)
                     /locus_tag="IVW29_000723"
     CDS             complement(6524..7471)
                     /locus_tag="IVW29_000723"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7838223.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7483..9369)
                     /locus_tag="IVW29_000724"
     CDS             complement(7483..9369)
                     /locus_tag="IVW29_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7838224.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9529..11607)
                     /locus_tag="IVW29_000725"
     CDS             complement(9529..11607)
                     /locus_tag="IVW29_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7838225.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11620..11895)
                     /locus_tag="IVW29_000726"
     CDS             complement(11620..11895)
                     /locus_tag="IVW29_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838226.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12257..12712)
                     /locus_tag="IVW29_000727"
     CDS             complement(12257..12712)
                     /locus_tag="IVW29_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838227.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12866..14074)
                     /locus_tag="IVW29_000728"
     CDS             complement(12866..14074)
                     /locus_tag="IVW29_000728"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7838228.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14186..14518)
                     /locus_tag="IVW29_000729"
     CDS             complement(14186..14518)
                     /locus_tag="IVW29_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7838229.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14666..15541
                     /locus_tag="IVW29_000730"
     CDS             14666..15541
                     /locus_tag="IVW29_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7838230.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15623..16204)
                     /locus_tag="IVW29_000731"
     CDS             complement(15623..16204)
                     /locus_tag="IVW29_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7838231.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16210..17469)
                     /locus_tag="IVW29_000732"
     CDS             complement(16210..17469)
                     /locus_tag="IVW29_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7838232.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17471..18517)
                     /locus_tag="IVW29_000733"
     CDS             complement(17471..18517)
                     /locus_tag="IVW29_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838233.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18752..19027)
                     /locus_tag="IVW29_000734"
     CDS             complement(18752..19027)
                     /locus_tag="IVW29_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7838234.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19270..20580)
                     /locus_tag="IVW29_000735"
     CDS             complement(19270..20580)
                     /locus_tag="IVW29_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7838235.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20762..21991)
                     /gene="rpsA"
                     /locus_tag="IVW29_000736"
     CDS             complement(20762..21991)
                     /gene="rpsA"
                     /locus_tag="IVW29_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7838236.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22126..22806)
                     /locus_tag="IVW29_000737"
     CDS             complement(22126..22806)
                     /locus_tag="IVW29_000737"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7838237.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22855..23490)
                     /locus_tag="IVW29_000738"
     CDS             complement(22855..23490)
                     /locus_tag="IVW29_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7838238.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23556..24959)
                     /locus_tag="IVW29_000739"
     CDS             complement(23556..24959)
                     /locus_tag="IVW29_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7838239.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(24946..25977)
                     /locus_tag="IVW29_000740"
     CDS             complement(24946..25977)
                     /locus_tag="IVW29_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838240.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26008..26229
                     /locus_tag="IVW29_000741"
     CDS             26008..26229
                     /locus_tag="IVW29_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7838241.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26394..27131)
                     /locus_tag="IVW29_000742"
     CDS             complement(26394..27131)
                     /locus_tag="IVW29_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7838242.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27284..28624)
                     /locus_tag="IVW29_000743"
     CDS             complement(27284..28624)
                     /locus_tag="IVW29_000743"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7838243.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28861..30384)
                     /locus_tag="IVW29_000744"
     CDS             complement(28861..30384)
                     /locus_tag="IVW29_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7838244.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30762..31373)
                     /locus_tag="IVW29_000745"
     CDS             complement(30762..31373)
                     /locus_tag="IVW29_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7838245.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31496..31621)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31703..32419)
                     /locus_tag="IVW29_000746"
     CDS             complement(31703..32419)
                     /locus_tag="IVW29_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7838246.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32425..32994)
                     /gene="scpB"
                     /locus_tag="IVW29_000747"
     CDS             complement(32425..32994)
                     /gene="scpB"
                     /locus_tag="IVW29_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7838247.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(32978..33775)
                     /locus_tag="IVW29_000748"
     CDS             complement(32978..33775)
                     /locus_tag="IVW29_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7838248.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33745..34116)
                     /locus_tag="IVW29_000749"
     CDS             complement(33745..34116)
                     /locus_tag="IVW29_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7838249.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34135..35022)
                     /gene="xerD"
                     /locus_tag="IVW29_000750"
     CDS             complement(34135..35022)
                     /gene="xerD"
                     /locus_tag="IVW29_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7838250.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         9838 a         7369 c         5971 g        11955 t
ORIGIN      
        1 aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa aaaaatttgc
       61 tcaaattgca aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga
      121 agaaatgttt tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat
      181 gaagatgtaa gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga
      241 tacgataagt attctccgcc tctatttttt ttattcgtac aatctctttt ttacagttta
      301 taaaagcagt cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa
      361 tgtgtcatga cacattggtg attttatggt atccttttat tattaactga caaaggagag
      421 aatattttga acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga
      481 ttgttgtcga cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt
      541 ggtcctgctt attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta
      601 tatggaccga tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata
      661 tttggttcag gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt
      721 aatggcttat ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta
      781 ttgattatga tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt
      841 gcagatttca aagtaatttt tctaggtaga ttcatcagga atatgatcca aatccctatt
      901 caaataatgg ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga
      961 gctgtttgat tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc
     1021 agtctcaaca gactgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg
     1081 gataatagac aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac
     1141 gactatcttt tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct
     1201 tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat
     1261 ctgatttcag gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt
     1321 gatcattttg gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg
     1381 tgccatctgc taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca
     1441 tcacaaccat atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg
     1501 ggatgaataa aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga
     1561 atacaagcca gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc
     1621 ctcttttttt cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc
     1681 tgaaataaaa ataattcttt caattaggct attggacaaa aaactttttt gatcataaag
     1741 tacaaaacta tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca
     1801 aagatatcca tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg
     1861 tagctagagc tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat
     1921 aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc
     1981 acccttcgtt cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa
     2041 aattttcagg atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt
     2101 gggctggttc gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt
     2161 tcttactttt ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat
     2221 aattgtcacc gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt
     2281 aaagttctac taattgttca tatgaaatca atgttggatc atagataatc tccactgctt
     2341 ctgtatgtcc agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc
     2401 cagtatatcc tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac
     2461 accagaagca gcctcctgca aatattgctc gttccattat gattccccct ctaatactac
     2521 tcatccgatg attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg
     2581 ttggtatttg agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga
     2641 atgtcatcat ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg
     2701 aattgatcca acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt
     2761 aatttatagt ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg
     2821 atagaaagac ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa
     2881 tataaataga aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca
     2941 ttttccaaat aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag
     3001 ctgatgattt gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt
     3061 ctttccgtaa gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg
     3121 cctaaaaata cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag
     3181 taatttgttt tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat
     3241 catcctcctt ctctaaccat tggttttgtg tctcgatcag ctgatctctc actgcatttg
     3301 ccatcaattg ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat
     3361 ttttgatgcc atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga
     3421 cacctacttc ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt
     3481 tcggcatttt ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca
     3541 tgtcagtgat ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag
     3601 gtgcggtttt attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt
     3661 ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt
     3721 tcatcaaatc attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc
     3781 gaatttcttc ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac
     3841 catagttttc tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa
     3901 ggggaacgaa tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga
     3961 tagctatgta gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt
     4021 tcattagtgg gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa
     4081 ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt ttttcacctc
     4141 ttgtattcga ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa
     4201 aaagaaaaca attattttcc taattttata aagaaaaaga gaataagctg caagcctttc
     4261 aggctgaggg cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct
     4321 ttaccagtat gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg
     4381 aataattctt gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa
     4441 ataccgattt gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat
     4501 ttatcgaacg ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa
     4561 ttcatcacca cgcgtatgtt caaaacacta gacagcaaac cagttgttcg gctgattctt
     4621 ccacctttta ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt
     4681 acttttttga cttcttcaag gattttctct actgttttac cttcttttgc taattcagct
     4741 gctcgaatta cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact
     4801 ttcgtttttg ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt
     4861 gtcatatgga tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagatg
     4921 aattctccaa ttggtggttg gcttgttttg ggcagatttt ttgctttcgc catcatttcc
     4981 ataaatcttt cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg
     5041 gatagcggca tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa
     5101 gaatctgtta cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg
     5161 ccttattcat gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa
     5221 tgatttttca ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa
     5281 tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg
     5341 attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct
     5401 ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac
     5461 tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag
     5521 ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg
     5581 atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata
     5641 taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat
     5701 ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac
     5761 tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag
     5821 cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg
     5881 ttttatgtac ttctctgttt tattctttac ttaataaaaa aaataaagct atggatggga
     5941 cagctctaaa taacagctag tctatccata gcttttttat atcgtcaatt cgcctgtccc
     6001 gctttttaca ggattctcat tacgctgata cgtttcaaat tgatgcggat aacgattttt
     6061 ttcatccatt tctccttgac tagtattgat cagtgaccaa tcagaccagt caacaggtgg
     6121 aaaataagca tctccatcga atacatgatg gatgactgta cggtataata tcgtacaaaa
     6181 tggcagaaat tcttgataaa cagctgaccc tccagcgatg aaaaagatac cgtctgtttc
     6241 tttttccaga gcaagcacgt cttccacact atgcatgaca tgcacgcctt ctgctttata
     6301 ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc aacggacggc tacccatccc
     6361 ttcgaatgtt tttcgtccca tcactagtgt atttgcttct gtcatttgtt tgaagaattt
     6421 cagatcattt ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc cgttttcatc
     6481 ctgtgcccaa attgcagcta acattttctc atcctttctg attctaaaca gcgataggag
     6541 ctttgatcgc cggatgtggg tcatatcctt ctaattggat atcttccatc tcaaaatcaa
     6601 atactgactg cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca
     6661 attgttcttt catttgttcg atatgattga tataaagatg agcatctcct aatgtgtgaa
     6721 caaattcgcc aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc aacgcataac
     6781 ttgcaatgtt gaacggaacg cctagaaaca catccccact gcgttgatac aattggcagc
     6841 ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg acaaggaggc aaagccatgc
     6901 taggaacatc ttccggattc catgcagaaa cgatcaatct tctagaatca ggagtctttt
     6961 tgattgcttc gatcacttgt ttgatttgat cgataaagcc accgtctttt gtttcccagt
     7021 gacgccattg atagccgtag atgtgtccaa gatcgccgta tgtttcagca aaggaatcat
     7081 cggttaggat cttctcgcaa aaaagcttgc tctgcttttt atattcttca ttgaattcag
     7141 gatctgtcaa acatctatgg ccaaaatcag tcatatctgg tccattatag tctggacttt
     7201 tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt tttattttgt aatagatagc
     7261 ggatattcgt atctcctttt aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta
     7321 ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt tggtatccga
     7381 ataagcttaa tgtgcctgtg cctgttctgt cttctttacg atgcccttct tccaacaatt
     7441 ttcttcctaa gtcaagatac gcttgttcca tcctttttcc tcctaatctt ctacccattc
     7501 actcaagtat tcccaccgtg ccattttttc ttcaagttgt gcttctgttt cagatacgtc
     7561 gttttgcaat tcttgtaatc gagtgaaatt atctccttga tgattcatct cttcttggag
     7621 caatgagatt ttttcttcaa gttctgcaat ctcatcttca atcgtttccc attctttttg
     7681 ttccatgtaa gataatttct tcttttcagc aggttccttg ctctgtattt ttggtttcgc
     7741 tttttcagca ggctgctggg tctctttttg cttcagtaaa taactggaca tgtttccgta
     7801 ataaagttct tgttttccgt ttccttggaa tactaataac ttatctgcta ccttgtccaa
     7861 gaaataacgg tcatgggaaa cagtgatcac agcacctgga aaacttctaa aataatcttc
     7921 caaaatcgtc aatgttgcta tgtccaaatc attggtcggt tcatccaata ataaaacatt
     7981 aggttgctga atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt
     8041 gcctatcagt gtcccatgca taaatcttgg aaataaaaat cgttcaagta attctgctac
     8101 tccgatttga ctgccatctg ctgtttttgc ttcttcagca gcttcttgta agtaagcaat
     8161 catccgttta tctggtgcca tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt
     8221 ttcaccgata gacattgtac cgctatccag gggaattctg ccagctagaa tattaagcaa
     8281 tgtcgatttt cccgcaccat ttttaccagt aatacctagt ctttcccgag attgaatcaa
     8341 taaatctaaa tgttctagca gtgtttggtc atttatacgg taactgccat ctttgatttc
     8401 cagtactttt ttccctaaac gttgtgtcgc taggttcagc tctaaatcat cttcttggtt
     8461 tacttggtac aagttctcct ttagatcttc gaaacggttg atccgtgctt gctgcttggt
     8521 gccacgtgcc tgtacgccag ccctcatcca agctaattcc tgtttataaa gccgcttgcg
     8581 cttttcttct tgttctactt ctacacgatc acgttctgct ttttctaaga cgtaagcttc
     8641 ataatttcct ttatactcgt ataattttcc aaatgaaagt tcgaagattc ggtttgctac
     8701 tctatctaga aaataacgat cgtgggtaac catcaatagt gcgccacggt attgtttcaa
     8761 atagttttcc aaccactcga tcgcttcata atctaaatgg tttgttggtt cgtctagtaa
     8821 tagtaaatcg ggttcatcaa tcaacacttg cgccagacta actcgttttt tctgaccacc
     8881 tgataaagta ctgatttttt tatctaattc actgattcct aatttttgca aaataatctt
     8941 tgcgttggta tctgtagtcc aagcatcttc tttattcatt tgttcttctg ctttagcata
     9001 acgtttttgc acatcagggt cttctccgtt ttcccctagt tcgatcaatg cttcctcata
     9061 tgctcgaaca gtttggatca aggggctgtt tccttgaaag actgcttgga gaacagttaa
     9121 ttcatcggaa aattcagctg cttgagaaag ataaccaata cggtaatcag ttggataaaa
     9181 cactgtttgc cgatcaccgt cgctgctatc gataccggca ataatattta agagacttgt
     9241 tttacccgtc ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga
     9301 aatctggtca aacaaatctt tttcaccata agtttttttg aggtcagtta cttttaattc
     9361 tttcaacatg ttccctcctt atcttggata ttgtatcata aaaatagtga acttcaggaa
     9421 aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc
     9481 tgttctgata tgttttgttt tttataaaac ttgaaaattt ttaccttttt attttattgg
     9541 actcgttttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt
     9601 gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt
     9661 cgtgattttt tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct
     9721 tagcattttt ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc
     9781 cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc
     9841 atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata
     9901 tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct
     9961 tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa
    10021 ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa
    10081 acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa
    10141 tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc
    10201 atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc
    10261 ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac
    10321 ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt
    10381 ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc
    10441 cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa
    10501 aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat
    10561 ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa aaagactgat
    10621 agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag
    10681 accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc
    10741 taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga
    10801 caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat
    10861 acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc
    10921 tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt
    10981 ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc
    11041 cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat
    11101 cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc
    11161 tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac
    11221 tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac
    11281 acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc
    11341 aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc
    11401 tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc
    11461 gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg
    11521 agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa
    11581 tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag
    11641 catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt
    11701 ttggttcccc tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt
    11761 cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg
    11821 acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc
    11881 aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt
    11941 ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata
    12001 aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa
    12061 gataaaagtt tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta
    12121 tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa
    12181 cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt
    12241 ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg
    12301 gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat
    12361 tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat
    12421 ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc
    12481 ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga
    12541 tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc
    12601 caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc
    12661 aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga
    12721 ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg
    12781 gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag
    12841 ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa
    12901 cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag
    12961 cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta
    13021 ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca
    13081 tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc
    13141 ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga
    13201 caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac
    13261 gaatctcatt ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt
    13321 ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca
    13381 aaccgggaca gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt
    13441 catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat attttaccta
    13501 atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt
    13561 gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag
    13621 gattgcctac agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga
    13681 tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat
    13741 cttctgccaa cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg
    13801 attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc
    13861 catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg
    13921 cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc
    13981 cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct
    14041 tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct
    14101 tgtcctgaga taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt
    14161 caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc
    14221 tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat
    14281 tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg
    14341 gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac
    14401 ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt
    14461 gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct
    14521 ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga
    14581 gaaaatcgaa tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa
    14641 tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt
    14701 tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca
    14761 tttagcagaa gggggagtct cagggatcac cttgatctta agagctttat ttggtattga
    14821 tccagcttat acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct
    14881 cggtaaacgt tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg
    14941 gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct
    15001 acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac
    15061 aggcggcagt gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg
    15121 ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa
    15181 acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt
    15241 agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc
    15301 accactactg atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt
    15361 ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc
    15421 taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga
    15481 agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga aaaaactata
    15541 agaaaaaaga caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt
    15601 tttagtacta tactattttt aactaattgt tatgttcact tacccggtta cttttctttt
    15661 gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat
    15721 ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt
    15781 ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc
    15841 aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt
    15901 cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct
    15961 ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag
    16021 atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat
    16081 tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc
    16141 taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta
    16201 gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg
    16261 ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct
    16321 taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg
    16381 ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt
    16441 ccattctgca taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc
    16501 atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc
    16561 tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa
    16621 agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc
    16681 ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata
    16741 tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc
    16801 ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt
    16861 cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat
    16921 acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct
    16981 tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa
    17041 ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa
    17101 ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa
    17161 gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc
    17221 gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg
    17281 gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag
    17341 caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa
    17401 aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt
    17461 tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc
    17521 tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa
    17581 caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg
    17641 aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt
    17701 ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta
    17761 aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt
    17821 caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt
    17881 cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat
    17941 acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt
    18001 caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg
    18061 gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg
    18121 ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg
    18181 ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga
    18241 taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg
    18301 ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa
    18361 ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga
    18421 ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt
    18481 tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct cttcttattt
    18541 ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact
    18601 tggaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg
    18661 gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag
    18721 ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac
    18781 ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt
    18841 tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa
    18901 ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag
    18961 ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt
    19021 ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa
    19081 actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat
    19141 cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt
    19201 agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg
    19261 gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa
    19321 tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc
    19381 attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt
    19441 caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc
    19501 attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc
    19561 aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc
    19621 aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt
    19681 ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta
    19741 acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac
    19801 gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc
    19861 gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact
    19921 ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat
    19981 tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc
    20041 aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt
    20101 cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg
    20161 atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac
    20221 tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc
    20281 atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc
    20341 aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat
    20401 tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg
    20461 atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt
    20521 aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat
    20581 attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt
    20641 caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt
    20701 ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca
    20761 attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta
    20821 aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt
    20881 ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat
    20941 gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt
    21001 catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc
    21061 cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata
    21121 ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga
    21181 ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat
    21241 cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat
    21301 ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa
    21361 cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt
    21421 ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga
    21481 tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat
    21541 gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc
    21601 ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga
    21661 tatcttccca aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt
    21721 ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta
    21781 cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa
    21841 ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct
    21901 taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt
    21961 tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc
    22021 cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt
    22081 tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata
    22141 accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt
    22201 cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc
    22261 ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt
    22321 ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat
    22381 tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc
    22441 accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc
    22501 agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt
    22561 tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt
    22621 gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata
    22681 agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa
    22741 tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg
    22801 tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt
    22861 aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc
    22921 aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc
    22981 ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt
    23041 gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc
    23101 tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat
    23161 tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact
    23221 ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc
    23281 atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa
    23341 aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga
    23401 aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc
    23461 tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta
    23521 ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat
    23581 aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt
    23641 ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt
    23701 ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga
    23761 agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa
    23821 ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag
    23881 ggagcttgct ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa
    23941 agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca
    24001 ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct
    24061 ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat accaaatgca
    24121 ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt
    24181 tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga
    24241 tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt
    24301 tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata
    24361 ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt
    24421 atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct
    24481 aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga
    24541 gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct
    24601 aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga
    24661 tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt
    24721 ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac
    24781 acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc
    24841 gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct
    24901 tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt
    24961 ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa
    25021 ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc
    25081 caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa
    25141 ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat
    25201 ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa
    25261 attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat
    25321 ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt
    25381 gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata attggttagc
    25441 taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc
    25501 agtatcaatt aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa
    25561 agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga
    25621 aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg
    25681 gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg
    25741 cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa
    25801 aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc
    25861 gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag
    25921 agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt
    25981 attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc
    26041 tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa
    26101 ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa
    26161 gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat
    26221 aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata
    26281 agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc
    26341 tatctatagt taattgacta gttttttcac ggaccctata ggacccattt atattatttc
    26401 aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa
    26461 atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg
    26521 ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct
    26581 tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct
    26641 gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg
    26701 tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg
    26761 atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc
    26821 actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct
    26881 tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa aataagtaat
    26941 ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt
    27001 ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag
    27061 taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta
    27121 aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat
    27181 gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga
    27241 cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac
    27301 gatacacagc ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt
    27361 cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact
    27421 ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag
    27481 taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata
    27541 atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta
    27601 atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt
    27661 ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt
    27721 catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta
    27781 agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag
    27841 cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt
    27901 caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct
    27961 tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag
    28021 aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa
    28081 ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat
    28141 atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg
    28201 tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag
    28261 gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga
    28321 atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga
    28381 taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt
    28441 taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca
    28501 cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc
    28561 ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt
    28621 tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc
    28681 tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac
    28741 aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg
    28801 atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt
    28861 ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa
    28921 tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat
    28981 tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt
    29041 gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt
    29101 tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa
    29161 tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa
    29221 gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac
    29281 tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga
    29341 ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata acatagtaat
    29401 ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac
    29461 cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt
    29521 tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac
    29581 atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt
    29641 catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga
    29701 tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg
    29761 aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa
    29821 aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc
    29881 tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat
    29941 cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg
    30001 aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag
    30061 tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat
    30121 tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt
    30181 gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga
    30241 tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt
    30301 aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa
    30361 ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat
    30421 tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt
    30481 tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt
    30541 taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc
    30601 ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca
    30661 aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt
    30721 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
    30781 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
    30841 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
    30901 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
    30961 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
    31021 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
    31081 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
    31141 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
    31201 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
    31261 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
    31321 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
    31381 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
    31441 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
    31501 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
    31561 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
    31621 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
    31681 aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
    31741 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
    31801 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
    31861 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
    31921 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
    31981 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
    32041 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
    32101 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
    32161 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
    32221 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
    32281 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
    32341 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
    32401 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
    32461 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
    32521 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
    32581 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
    32641 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
    32701 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
    32761 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
    32821 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
    32881 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
    32941 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
    33001 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
    33061 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
    33121 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
    33181 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
    33241 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
    33301 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
    33361 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
    33421 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
    33481 attcttcatc gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
    33541 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
    33601 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
    33661 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
    33721 taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc
    33781 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
    33841 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
    33901 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
    33961 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
    34021 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
    34081 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
    34141 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
    34201 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
    34261 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
    34321 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
    34381 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
    34441 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
    34501 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
    34561 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
    34621 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
    34681 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
    34741 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
    34801 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
    34861 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
    34921 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
    34981 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
    35041 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
    35101 aaaagccgtc tagacactca gacggactgg ttt
//
LOCUS       DACCVU010000018        34962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCVU010000018 DACCVU010000000
VERSION     DACCVU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.018"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            156..1862
                     /locus_tag="IVW29_000751"
     CDS             156..1862
                     /locus_tag="IVW29_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7838251.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVW29_000752"
     CDS             complement(2020..2922)
                     /locus_tag="IVW29_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7838252.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVW29_000753"
     CDS             complement(2919..3623)
                     /locus_tag="IVW29_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7838253.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVW29_000754"
     CDS             complement(3654..4295)
                     /locus_tag="IVW29_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7838254.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IVW29_000755"
     CDS             complement(4333..4647)
                     /locus_tag="IVW29_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838255.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IVW29_000756"
     CDS             complement(4635..5264)
                     /locus_tag="IVW29_000756"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7838256.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVW29_000757"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVW29_000757"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7838257.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IVW29_000758"
     CDS             complement(5984..6916)
                     /locus_tag="IVW29_000758"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7838258.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IVW29_000759"
     CDS             complement(6913..7689)
                     /locus_tag="IVW29_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7838259.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVW29_000760"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVW29_000760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7838260.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVW29_000761"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVW29_000761"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7838261.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IVW29_000762"
     CDS             complement(12014..13294)
                     /locus_tag="IVW29_000762"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7838262.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IVW29_000763"
     CDS             complement(13313..14239)
                     /locus_tag="IVW29_000763"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7838263.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IVW29_000764"
     CDS             complement(14252..15535)
                     /locus_tag="IVW29_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7838264.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVW29_000765"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVW29_000765"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7838265.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IVW29_000766"
     CDS             complement(16323..17234)
                     /locus_tag="IVW29_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7838266.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IVW29_000767"
     CDS             complement(17231..17695)
                     /locus_tag="IVW29_000767"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7838267.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IVW29_000768"
     CDS             complement(17700..18398)
                     /locus_tag="IVW29_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7838268.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IVW29_000769"
     CDS             complement(18385..18771)
                     /locus_tag="IVW29_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838269.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IVW29_000770"
     CDS             complement(18918..20588)
                     /locus_tag="IVW29_000770"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7838270.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IVW29_000771"
     CDS             20771..21004
                     /locus_tag="IVW29_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7838271.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IVW29_000772"
     CDS             complement(21103..21534)
                     /locus_tag="IVW29_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7838272.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IVW29_000773"
     CDS             21613..22026
                     /locus_tag="IVW29_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7838273.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IVW29_000774"
     CDS             complement(22064..23212)
                     /locus_tag="IVW29_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7838274.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="IVW29_000775"
     CDS             complement(23334..23930)
                     /locus_tag="IVW29_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7838275.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVW29_000776"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVW29_000776"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7838276.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="IVW29_000777"
     CDS             27160..27591
                     /locus_tag="IVW29_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838277.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="IVW29_000778"
     CDS             complement(27636..28985)
                     /locus_tag="IVW29_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7838278.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="IVW29_000779"
     CDS             complement(29055..30218)
                     /locus_tag="IVW29_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838279.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="IVW29_000780"
     CDS             30347..30670
                     /locus_tag="IVW29_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838280.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="IVW29_000781"
     CDS             complement(30853..32202)
                     /locus_tag="IVW29_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838281.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="IVW29_000782"
     CDS             complement(32271..33224)
                     /locus_tag="IVW29_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7838282.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="IVW29_000783"
     CDS             complement(33326..34516)
                     /locus_tag="IVW29_000783"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7838283.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggtatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DACCVU010000019        34440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCVU010000019 DACCVU010000000
VERSION     DACCVU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.019"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVW29_000784"
     CDS             352..1041
                     /locus_tag="IVW29_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838284.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVW29_000785"
     CDS             1028..2236
                     /locus_tag="IVW29_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7838285.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVW29_000786"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVW29_000786"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7838286.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVW29_000787"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVW29_000787"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7838287.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVW29_000788"
     CDS             complement(6339..7034)
                     /locus_tag="IVW29_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7838288.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVW29_000789"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVW29_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7838289.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVW29_000790"
     CDS             complement(8233..8424)
                     /locus_tag="IVW29_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7838290.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVW29_000791"
     CDS             complement(8445..9335)
                     /locus_tag="IVW29_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7838291.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVW29_000792"
     CDS             complement(9322..10089)
                     /locus_tag="IVW29_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7838292.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVW29_000793"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVW29_000793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7838293.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVW29_000794"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVW29_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7838294.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVW29_000795"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVW29_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7838295.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVW29_000796"
     CDS             14705..15043
                     /locus_tag="IVW29_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838296.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVW29_000797"
     CDS             complement(15211..15969)
                     /locus_tag="IVW29_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7838297.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVW29_000798"
     CDS             complement(15987..16805)
                     /locus_tag="IVW29_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7838298.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVW29_000799"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVW29_000799"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7838299.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVW29_000800"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVW29_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7838300.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVW29_000801"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVW29_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7838301.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IVW29_000802"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IVW29_000802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7838302.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20843..21085
                     /gene="yaaA"
                     /locus_tag="IVW29_000803"
     CDS             20843..21085
                     /gene="yaaA"
                     /locus_tag="IVW29_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7838303.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21072..22196
                     /gene="recF"
                     /locus_tag="IVW29_000804"
     CDS             21072..22196
                     /gene="recF"
                     /locus_tag="IVW29_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7838304.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22193..24139
                     /gene="gyrB"
                     /locus_tag="IVW29_000805"
     CDS             22193..24139
                     /gene="gyrB"
                     /locus_tag="IVW29_000805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7838305.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24159..26630
                     /gene="gyrA"
                     /locus_tag="IVW29_000806"
     CDS             24159..26630
                     /gene="gyrA"
                     /locus_tag="IVW29_000806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7838306.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26866..27165
                     /locus_tag="IVW29_000807"
     CDS             26866..27165
                     /locus_tag="IVW29_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7838307.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27215..27727
                     /gene="ssb"
                     /locus_tag="IVW29_000808"
     CDS             27215..27727
                     /gene="ssb"
                     /locus_tag="IVW29_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7838308.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27753..27989
                     /gene="rpsR"
                     /locus_tag="IVW29_000809"
     CDS             27753..27989
                     /gene="rpsR"
                     /locus_tag="IVW29_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7838309.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28132..30105
                     /locus_tag="IVW29_000810"
     CDS             28132..30105
                     /locus_tag="IVW29_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7838310.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30111..30563
                     /gene="rplI"
                     /locus_tag="IVW29_000811"
     CDS             30111..30563
                     /gene="rplI"
                     /locus_tag="IVW29_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7838311.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30837..32204
                     /gene="dnaB"
                     /locus_tag="IVW29_000812"
     CDS             30837..32204
                     /gene="dnaB"
                     /locus_tag="IVW29_000812"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7838312.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32984..34276
                     /locus_tag="IVW29_000813"
     CDS             32984..34276
                     /locus_tag="IVW29_000813"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7838313.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10508 a         6696 c         6818 g        10418 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca cctttttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa
    20761 aaatatatgg ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa
    20821 taatgaaaat gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac
    20881 gttgggccaa atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta
    20941 tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact
    21001 ttatcccggc atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg
    21061 aaacgacgat aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc
    21121 tgaacatcga attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa
    21181 cgaatctttt ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg
    21241 agcaggaatt gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag
    21301 gttcatccac tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca
    21361 accatatcga acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg
    21421 caccagaaga tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata
    21481 tggaattagg ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt
    21541 tgaaacaaag aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt
    21601 atttggatat tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag
    21661 cacagttcgt caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc
    21721 aaaaagagca gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc
    21781 aagagcaaat ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt
    21841 ttcggggaac aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga
    21901 aaaatgtgca gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat
    21961 tagcagaaat cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg
    22021 atgtgatgag cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa
    22081 aagttcaaac gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg
    22141 aacctgagat tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga
    22201 agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga
    22261 aggacttgaa gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg
    22321 tctgcatcac cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt
    22381 tgcaacgaaa atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg
    22441 tcgggggata cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt
    22501 tactgtttta catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact
    22561 tcacggagta ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata
    22621 caaagaagga aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa
    22681 gattatcgaa caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat
    22741 ttttacagag acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc
    22801 tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat
    22861 caatgaatac cattatgacg gcgggatcaa aagttatgtc gaatacttga atgcaaacaa
    22921 aaccgtgatt tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga
    22981 agtctctatg caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat
    23041 ccatacgtat gaaggtggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat
    23101 aaatgattat gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga
    23161 agacgtgcga gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga
    23221 aggacaaaca aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt
    23281 tagcgaacat ttcatgaaat tcttgctgga aaacccaagt gtgggccggc aaattgttga
    23341 aaaaggatta ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag
    23401 acgtaaaggt gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa
    23461 cgatcctgaa aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa
    23521 gcaaggtcgt aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt
    23581 tgaaaaagca agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc
    23641 tatgggtaca gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt
    23701 tatcatgact gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt
    23761 ttatcgttac atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt
    23821 atacggggta aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga
    23881 agagttgaaa caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta
    23941 taaaggactt ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa
    24001 tcggatgatg gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat
    24061 gttgatgggg gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt
    24121 gaagaactta gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa
    24181 acatccatga tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga
    24241 gtgttattgt cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc
    24301 gtattttata cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg
    24361 ccagaatcgt cggagatgtc atgggtaaat accatcctca tggagacatt gcgatctatg
    24421 agtcaatggt acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg
    24481 gaaactttgg ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga
    24541 tgagcaaaat cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa
    24601 gcaactatga tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt
    24661 tagtgaacgg aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact
    24721 tgtctgaggt agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg
    24781 aattgatgga agtattacct ggaccagatt ttccaacagg tggtttggtt atggggaaat
    24841 caggaatccg aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg
    24901 agattactga gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg
    24961 tcaataaagc aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag
    25021 ggatcacaga tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc
    25081 gtcgtgacgc cagcgcatcc gttatcttga ataacttata taaattgaca tccttgcaaa
    25141 attcatttgg attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga
    25201 aacaaatcct tgaaaactat atcgaacatc aacgcgaagt catcacacga cggacggtct
    25261 ttgaaaaaga aaaagcagaa gcaagagcac acatcttaga aggtttgcgt attgctttgg
    25321 atcatatcga tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaggcaaaag
    25381 caacaatgat cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc
    25441 gtttacgccg attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct
    25501 taaaactgat tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca
    25561 tcaagaatga attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac
    25621 tagtcggtga ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta
    25681 ttacattgac aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac
    25741 gaaggggtgg acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc
    25801 tggtttcttg ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc
    25861 gggcaaaagg atacgaaatt ccagaatacg ggcgtacagc caaaggaatc ccaatcatca
    25921 atttactagg tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag
    25981 cagaagaagg ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg
    26041 tcactgcttt tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg
    26101 atgagctcgt caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata
    26161 acggctactc tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg
    26221 gggtcagagg gatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg
    26281 aaaatcaaga agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg
    26341 aatatccagt gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa
    26401 atggtccact agctggattg accactgttt ctggagatga agacatcatg ctgattacag
    26461 ataaaggtgt cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat
    26521 taggcgtacg tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg
    26581 aaccagaagc tgcagaacaa acagttgaag aacaaggatc agaagaataa ccatcaagac
    26641 taagtgtgta agctagcaag aataaccgat taaaaaatct ggttattcct gctggctttt
    26701 ttgattagtt gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc
    26761 aaaatagagt gtataattag agcttgtgag tagtctaatt aattagataa ctctccttgc
    26821 tcttaggaac ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa
    26881 tatgaaatca tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc
    26941 gaacgtttcg atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg
    27001 gaaaaacgcc gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt
    27061 aaagtatctt ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc
    27121 aacgacgata tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg
    27181 aaacaataga aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg
    27241 acgtctgacg aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt
    27301 tactttggct gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat
    27361 caactgtgtg atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac
    27421 acttttaggt gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg
    27481 tgtatacgtg acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc
    27541 tgaaaatcgc caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg
    27601 cggaaacaat aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg
    27661 tgatacttct gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc
    27721 attctaaacg aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta
    27781 aacgtcgtaa agtagactac attgctgcta accatattga atatatcgat tataaagata
    27841 tcgaattact aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg
    27901 gtacaggcgc taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg
    27961 gcttgctacc tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt
    28021 tgatcagcta ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg
    28081 aatgacgaaa gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat
    28141 aaaaacaatc atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta
    28201 ttggtagtct taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat
    28261 ttatggggtg ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa
    28321 aaaatccagc aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg
    28381 cctgtaggag tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta
    28441 gcattgaaaa tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt
    28501 actgcaaaag aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat
    28561 atggatacag taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa
    28621 ctccatcaga cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat
    28681 gccattgata aattagatga caaagagatt tcttatttga actctttcgt cactacacta
    28741 gtttctgact ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt
    28801 ttcttcgtag cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg
    28861 gatcgctttc gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt
    28921 gcttatggct caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt
    28981 gctttagtcc gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct
    29041 gtttattatg gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg
    29101 atgagtaccg ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac
    29161 aagtttccgg atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc
    29221 aaccagaaaa aagcttacat cgttatcaat gagaacgaga tcattccaga tgtagaacgg
    29281 tgtcttgaag aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt
    29341 gccatagagc tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct
    29401 ctatcgatct ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat
    29461 cgtcgtgggg aagagtttcc aagtaaacct ctgctgactt atattgaatc ttcagcttct
    29521 tcagcttcag agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta
    29581 gatcgttttg aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat
    29641 gtccgtacaa ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac
    29701 gcatccttag tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat
    29761 ttgattgctg gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa
    29821 aaagagtata atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat
    29881 atcaatgcag ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga
    29941 agtaccggca cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc
    30001 acaaacgcag ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt
    30061 gctgcaatca aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa
    30121 tttttttaca agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag
    30181 gttatgcaca aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta
    30241 tcagtcagtt agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag
    30301 aagctaaaaa gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga
    30361 aagcagggga agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt
    30421 taaacaaaca atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc
    30481 gttcactagg ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca
    30541 aagttcatgt cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac
    30601 cagaaggaaa aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa
    30661 aagctgcaaa tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat
    30721 aaagaaaacg agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat
    30781 gacttttctc tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga
    30841 atgaagtgtg gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag
    30901 gctctgtttt tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag
    30961 acttttatcg tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta
    31021 atgaagcgat tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag
    31081 atgccggtgg tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata
    31141 tcgtctacta tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag
    31201 caacaggaat cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg
    31261 atgccgaacg cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta
    31321 ttgctgaagt actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag
    31381 aaatcacagg tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc
    31441 ccgaagaact aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga
    31501 atattgctca aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa
    31561 tgggggcaga atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc
    31621 atttacgtac aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga
    31681 gtctttctcg agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc
    31741 gtgcgaaatg tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt
    31801 atttgcagtt gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct
    31861 ctcgccaact gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt
    31921 tatctcgtgg agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat
    31981 ccggatcaat tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg
    32041 aaagagaagg cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag
    32101 tcatcatcga gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag
    32161 aatacaataa gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc
    32221 cttgagcaat cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag
    32281 agtacttaat ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt
    32341 tttccgaata aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa
    32401 tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg
    32461 acctcttttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat
    32521 caaacatgag aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct
    32581 cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa
    32641 acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct
    32701 ttttcacaac ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa
    32761 tttcacaaaa atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca
    32821 cttctgttac aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc
    32881 gtttttataa aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg
    32941 atacaatggt tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt
    33001 agtaggtacg caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa
    33061 tgctgaagtg attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa
    33121 cggtgtgact tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag
    33181 tgtgatcgga aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt
    33241 acatgaaaaa ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt
    33301 gccttatcat atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg
    33361 tacaacgatt aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg
    33421 tattgcagac ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga
    33481 caaaaaccgt caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt
    33541 tgaagaatat tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat
    33601 cttgaatgat gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat
    33661 gctggatatc gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg
    33721 ggtaacaatc ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa
    33781 agcctatacg tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg
    33841 agaacaaatc cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt
    33901 aggctggttt gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct
    33961 atctttaaac tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta
    34021 tgaattagat ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg
    34081 caaaccaatc tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact
    34141 tgcagaactg ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt
    34201 acgtatctca acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt
    34261 ctgggcgcaa atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag
    34321 tcgaagtgtt tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc
    34381 cttattgccg ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa
//
LOCUS       DACCVU010000020        33818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCVU010000020 DACCVU010000000
VERSION     DACCVU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.020"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(25..1044)
                     /locus_tag="IVW29_000814"
     CDS             complement(25..1044)
                     /locus_tag="IVW29_000814"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7838314.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1677..2681)
                     /locus_tag="IVW29_000815"
     CDS             complement(1677..2681)
                     /locus_tag="IVW29_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7838315.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2694..3743)
                     /locus_tag="IVW29_000816"
     CDS             complement(2694..3743)
                     /locus_tag="IVW29_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7838316.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFFFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3760..4581)
                     /locus_tag="IVW29_000817"
     CDS             complement(3760..4581)
                     /locus_tag="IVW29_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7838317.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4568..5347)
                     /locus_tag="IVW29_000818"
     CDS             complement(4568..5347)
                     /locus_tag="IVW29_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7838318.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5364..5834)
                     /locus_tag="IVW29_000819"
     CDS             complement(5364..5834)
                     /locus_tag="IVW29_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7838319.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5846..6265)
                     /locus_tag="IVW29_000820"
     CDS             complement(5846..6265)
                     /locus_tag="IVW29_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7838320.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6346..9114)
                     /locus_tag="IVW29_000821"
     CDS             complement(6346..9114)
                     /locus_tag="IVW29_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7838321.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            9314..9538
                     /locus_tag="IVW29_000822"
     CDS             9314..9538
                     /locus_tag="IVW29_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838322.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10317..12032
                     /locus_tag="IVW29_000823"
     CDS             10317..12032
                     /locus_tag="IVW29_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838323.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12035..13798
                     /locus_tag="IVW29_000824"
     CDS             12035..13798
                     /locus_tag="IVW29_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838324.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13838..14374)
                     /locus_tag="IVW29_000825"
     CDS             complement(13838..14374)
                     /locus_tag="IVW29_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7838325.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(14697..15119)
                     /gene="mgsA"
                     /locus_tag="IVW29_000826"
     CDS             complement(14697..15119)
                     /gene="mgsA"
                     /locus_tag="IVW29_000826"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7838326.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            15328..16437
                     /gene="ugpC"
                     /locus_tag="IVW29_000827"
     CDS             15328..16437
                     /gene="ugpC"
                     /locus_tag="IVW29_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7838327.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            complement(16561..17190)
                     /locus_tag="IVW29_000828"
     CDS             complement(16561..17190)
                     /locus_tag="IVW29_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838328.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17641..18525)
                     /gene="rsmA"
                     /locus_tag="IVW29_000829"
     CDS             complement(17641..18525)
                     /gene="rsmA"
                     /locus_tag="IVW29_000829"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7838329.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(18518..19087)
                     /gene="rnmV"
                     /locus_tag="IVW29_000830"
     CDS             complement(18518..19087)
                     /gene="rnmV"
                     /locus_tag="IVW29_000830"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7838330.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(19080..19856)
                     /locus_tag="IVW29_000831"
     CDS             complement(19080..19856)
                     /locus_tag="IVW29_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7838331.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(20011..21333)
                     /locus_tag="IVW29_000832"
     CDS             complement(20011..21333)
                     /locus_tag="IVW29_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838332.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            21508..22095
                     /locus_tag="IVW29_000833"
     CDS             21508..22095
                     /locus_tag="IVW29_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838333.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(22574..24583)
                     /gene="metG"
                     /locus_tag="IVW29_000834"
     CDS             complement(22574..24583)
                     /gene="metG"
                     /locus_tag="IVW29_000834"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7838334.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            24851..25129
                     /locus_tag="IVW29_000835"
     CDS             24851..25129
                     /locus_tag="IVW29_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838335.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            25226..26047
                     /locus_tag="IVW29_000836"
     CDS             25226..26047
                     /locus_tag="IVW29_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7838336.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(26332..27342)
                     /locus_tag="IVW29_000837"
     CDS             complement(26332..27342)
                     /locus_tag="IVW29_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7838337.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(27354..28307)
                     /locus_tag="IVW29_000838"
     CDS             complement(27354..28307)
                     /locus_tag="IVW29_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7838338.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(28297..29367)
                     /locus_tag="IVW29_000839"
     CDS             complement(28297..29367)
                     /locus_tag="IVW29_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838339.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(29373..30416)
                     /locus_tag="IVW29_000840"
     CDS             complement(29373..30416)
                     /locus_tag="IVW29_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838340.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(30417..31358)
                     /locus_tag="IVW29_000841"
     CDS             complement(30417..31358)
                     /locus_tag="IVW29_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838341.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            31442..31789
                     /locus_tag="IVW29_000842"
     CDS             31442..31789
                     /locus_tag="IVW29_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7838342.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(31859..33520)
                     /locus_tag="IVW29_000843"
     CDS             complement(31859..33520)
                     /locus_tag="IVW29_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7838343.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         9877 a         6908 c         5992 g        11041 t
ORIGIN      
        1 gacgattaac tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc
       61 acgtaacatg atttcttttg ctgctttttt gatatcttct ccattataca aaacattata
      121 gattgtttga gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt
      181 tgtcgttgcc acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg
      241 tccctgtcct aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac
      301 gatcaagtcc cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac
      361 accaagacgg ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc
      421 gaatcctaat ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc
      481 agtttctacg ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag
      541 tttttgtaca taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc
      601 atgtactgcc acttcctcag catggctagg accagataat acaacaattg cctgtctttt
      661 ttcttctgga atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag
      721 tcctttactt gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc
      781 gactgaacga atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc
      841 ttctgccaat tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg
      901 ctgatttgta tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg
      961 gacatcatgc ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc
     1021 aagaactgcg attttttgtt tcatggatat tcctccaaat aaataattga taatttccat
     1081 tatagtatac atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact
     1141 tcttttatcg tttttacttg aaataatcaa tgatatgtta caatagtcta cattttattt
     1201 ttacactcgt tatgctttgc ttgttcctaa gaggaagcag ttttgacaat gttactacca
     1261 gcattactat taggaggtta ggaaacttct cctagtgagt attgatttaa aatacataag
     1321 agacagattg aacatttcta aatctgtcag gtcctgcaac ggacagattg tctctgttga
     1381 cggttgacat aggaactttt gttttagtca acattctgga tacaaaacgc tatgtaaact
     1441 ggcgcaactc gttgtacttg gacttttact acggatccat gcaacaacta aatagtacta
     1501 ttgcataaca aaaaatggtt ggcacccaca tgtcccatta aacagattat aagttgaact
     1561 gtagataatg caaaaaatgt gttttataac gtaattgatg ccaatatttt aaaaaaataa
     1621 agggagcagt tatcgctgct ctctttattt tttatattct ttttatccag tctatcctaa
     1681 taatcaaact gacgatagta gcggcgtatc tctaatggat ggcagttcaa ctgctccaca
     1741 tgagcatcga tgcgttgtgt cactacatgc atcaataaga aagacaaaac acgtccgcga
     1801 tatttttcac tgataccttc tactggatag tcttttgtgt ctatcaatgt ataattgctg
     1861 caaatttttg gcaaaagttt agccacacgt tcagataaca ctctctgttc atcttcgcct
     1921 aaaaataatg tgactggggt tgtttcttct acgatctcta acgtcccgtg aagaaactcg
     1981 gcagcatgga tagatttcga tggaagccag ctttgttctt cccagtaaca catggcatat
     2041 gaataaacag ctccccattg attccctgct ccaataaaat aatgcatact atcacgtcga
     2101 tgtttttctg caaatgataa gccaaatgca tctgcttttt tctctgcttc cactaaccct
     2161 gttggtaaat aacgttcgag ctgttcatag tattctgaat aagcttcgaa ttctccattc
     2221 aaatacatca atcgatctgc tgccataaaa aatttgattt gttctgttcc aggagctgga
     2281 taagtgacca caaaatcgca tagctgagac aacttactgt tcgctttatc gataaatcca
     2341 atcagtgtcg ctcctacttt cttgatttgt tctactgctt tcactacttc ttgagttgta
     2401 ccggtaacag aggacaaaat aacgatagaa tcttttgtta atcttttgtt tccagtagtg
     2461 taatattctg cagcatgctg tacaaagaca ggcaagttgc ttttcccatt catataagtg
     2521 acagcttgca tcgaggaagc atacgttcca ccgattccta gataatagac agcgtcaaat
     2581 ttttccgcat aaagtttatc gatgatttct tctacttgcg gacgtaaagc tagtgcgcct
     2641 tttattgctt tgatttgttc ttcttcattg aatttcagca tgatttccac ctcttataat
     2701 ttacttttca agactgtatc aaagtcttca aaaatctttt tgttcaagtc aataccttta
     2761 gctactgcgg ttttataagc gaatagttga aacggaacga caagcgctaa tacagacaga
     2821 tattcatcac attctattgc taatccaagt gttttctcgt ttcttgcttt ttttgtggta
     2881 atggtgagag tctctccaac gtaccgactc atatatcttt gcaacttttg cgaacgttcc
     2941 ttatttacac tttccccttc aataaagaat agcaaatgcg aagcatctgc ctccaggtat
     3001 gggccgtgca tataagcttc taactcaaat ccttgtgacg gaacgcggat tgtttcagtc
     3061 aatttcgtct ctgcttcttt agctgttccc cagtttggac catatccaat ggtgacgaaa
     3121 cgctgtgcta aacgaaataa actttgatat ccatcaaaaa attcttccgt tttttgaata
     3181 atcgctggta aatggttgat tattttgcgc aactgttgca tgtagtcttg ttctattttt
     3241 ttgctgattt tatttttact tattccaatt cctatcccta agagtagtaa ctgtaaaaca
     3301 gtcgcagaat agccttttgt aacgaagcct actgattcta ccccggcacc taaatcaata
     3361 atttgtgcag cttctaaagc aagtggactt tgcacgtcat ttgtgatagc aacaacaggt
     3421 aacccttgtt tttgtatcat ttttactgct tcaacagtag aagcactttt cccgctttgt
     3481 gacacagcaa tcacagtatc gattgaagga gaaagttttc cataattgaa aaaaaaaaaa
     3541 ggttcttgaa tatcgacagt aagatcacca taagattcca atgctggctt cgctgctaaa
     3601 caagcgttat aagatgaacc cgttgctaag atcagtaaat tcactgtttt tttcatatgt
     3661 tctcttgtcg aaaaatcatt ctttttcaag atcgtactta gtacttcctt ttcttcatag
     3721 atataatctt gcatcgtaaa cattttctct gctccttttt taaaaaatcc ctatccacga
     3781 accaaaaatt ccaaagaaag ctagtccgat caagatcgtt aatggtttca cttctttctt
     3841 caatagataa aacactatgc caaatgctcc aagacataag atatttggaa cgatatcatc
     3901 aaaaatactt tgaacagtca cagcattttc tcctgaaccg atttttaaag gaatattgat
     3961 ggtgatcatc gtggcagtca ttgctccaac gaccatcagt ccaataatag acgcgcctaa
     4021 tgtcaaactt tccatcatac cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg
     4081 atatccccaa cctgttgcta gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat
     4141 gaacaagata gggcctaaaa tatttccttg catggctaaa gaggtaccca cacctgtggc
     4201 aatcaatcgc aaagttcccc aaaagaaaga atctcctaac cctgcaagag gtcccatcaa
     4261 agatgttttg atactatcga tcgcagtcac atcaaattca ggatcatcct tattttcttc
     4321 ctccattgca gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa
     4381 ctctagatgc cgtttcaaag ctgctgccat ttcttctttc gactgatata actttttcaa
     4441 aatcggaatc attgcataaa cataagccat attcatctga cgttcataat tccaagaaaa
     4501 ctccatttga aaagaacgcc aaaatacgtg atttagctct tttttcgtta ttttgctgtt
     4561 tttgatctca gaattcttca tcttcttcta cctcccctgc agttgtttga agtggtagtt
     4621 gacggacatt cataatattg actaccacaa ctgctaaaat cgcaccaaaa atagctactc
     4681 cggtcacagg aatctctaag taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa
     4741 cttgtttgtt gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga atcaaccctg
     4801 tagctactga caaccccttt tgaacgaagt caggaatcat atttaataga tttccgatcg
     4861 tattgcttcc caccatataa gaaaccatta ccacaacacc aagcatcaag gaaagaccga
     4921 taccggcaag caagtgcata tgctcgactc ctttataatt tccttcttct gcataattgt
     4981 ctgctttatg gttcataatt ggaatcaaaa gccctaagta aatatttttc aatatcaatg
     5041 tcaacgtagc aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa
     5101 tagcaaaagc cgtccctaaa atcccaccgg tcacaacatc aggtggaatc gaagcaccta
     5161 ctgagaacga tccgataaat gctaactcta aagttgcgcc cataatgatc ccagttttta
     5221 catcacctaa cacgattcct gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg
     5281 tccctagtgc atattctgat tgtgcgacaa atgcaacaat tcctaataag actgcttgta
     5341 caatcattga ggttcttcct ttcttacaaa acttctctta catccacttt tttatctgct
     5401 ggtacttgac ggatttctac ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc
     5461 tcttctggta aaatatttac cgcttttccg atgctgcgag taccaggttt tgccttgatc
     5521 cctcctaagt tgatggtttt gatttctgga tacgcttctg ccagcttata tgcatcctcg
     5581 atcgattcca ctacgatgaa taacttatat ttatctgtca ccccgctttt caacgcggca
     5641 atcgagtcat caattgattt gatcactagt ttgactcctt gtggcttggc taatttgatc
     5701 gttgtctttc gtatctcatt cgtaggtaca tcatcattag caatcaaaat acagtctgcg
     5761 cctattgttt gtgtccatga aaaagctact tgtccgtgta ataaccgatg gtcgactctt
     5821 gtcaatacaa tcatttttct tcctcctaaa attcgtcttc ttctattttc tgattgagcc
     5881 gctcgttaca aaactggatc attttttttg aatcagttag cacctgctgg atcaaaacag
     5941 ttgtctctac ttcagcgtcc aaccgagata ctagctcaat caatagtggc aaattcaatc
     6001 cggcaacgag atagacatga tcccgattga tatattgtaa gaattcgttg ttgacacttc
     6061 cgccaaaaag atccgtcaga acaatgattt ctttatcttt gtacctcata aaaagctgcc
     6121 ttactgtttc cgctaaatca aaattctcat cagtatagca atcgaataca gtaatagaat
     6181 gatttttccc atagatcagc tctaatgaat ctaatatccc tgaagcaaat tttccatggg
     6241 aagctaagat aatttctcta tccacttttt catctcctct tttcacttac acatactaaa
     6301 aaagcaagtt gcatgccaaa attagtggca acaacttgct tatgttcaaa attcttcttc
     6361 tatcgagaga aactctgtat cttcactcaa aatatcatag atataggcaa tctcagaatt
     6421 ggggatcatg acactataat gctcttcaat gacactaaac gcagatttaa tagcagataa
     6481 cccttctttt tgacactgct tccatttctc taatccttga taggtttcga ttggcatttg
     6541 tcggatcaat cgttcaatga gacagctcac atggacatac aacgctaatt ttttgggatt
     6601 gctgatgctt cgattcaact gcacctctaa ttctctcata aaatgatcga tttctcccat
     6661 caccttctct gtatctagaa tagtcacgga atccagtact ttttccaatg aaaaattacg
     6721 aatgatgcgc tgattgaata tttccttctc tgaagcattc atagtagtgt ccaaccattc
     6781 agctagtaca gacatctttt cccccgagat gaggtcttct aatgaaagat agtcgatatt
     6841 ggtgatatca ggatctgtgg tgccaatcat tcctacgact tcgtatatgg aaaataatgg
     6901 gtcactgttt ttcttatctt ttaattgctg atattcatat gggatgattt tcaactgaca
     6961 agccgtaggt aagctttttt ctagcagttc acttaagtgg accgccgtac cgattccagt
     7021 gaagcaagta gtaatgagtg ctttgggttt gttttcctct ggatagagca gtcgccattc
     7081 tggctggatc tgtcgaacag tctcttctgc tatctcttct aatgatacat ttttttgcag
     7141 gcattcccct actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac
     7201 ggataactgt tttttaaaaa actgatggat ttctttcaga gatcccatgt ctactaatat
     7261 gatcaatcca tttgaaacat cgctgttttc gcaataatca aggatttctg aagcaatctg
     7321 ctgtggtgtg acatctagtg gcatatcaaa tgattcaaat agatcctctt gtaataaacg
     7381 atttactaca tttgccatac tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt
     7441 tggcagtcgt ttttcttttg accatttcgc tttttttaga tagatcgtca gtaaaatccg
     7501 atccatactt gtgtcttcaa tatctaattt gatcttactt aattccagaa ttcgttggac
     7561 atagtgataa ctagatggat aagactgaga aacttgttgt tctaattctt tgattagttg
     7621 gataatatca tgttcttccg gtaaccattt gatagtgctt cgctgataga gatagtaact
     7681 caaggcatag acactgtttc catcgaattt gatttgatag gcagtttcca tttgtcgaaa
     7741 ggtctctcgg atagattgtg tccaataaac cagcagctcg tgtttttctt gtctactggt
     7801 ctcaaacagc aaaaaatcaa ataattgaat cacttcttgc tttaattttc cttcgcaaga
     7861 atatagcaga cagttatttt tcttgaaatc aattaggatt tgctcaaaac ttttaacgat
     7921 tcgctgctgt tcgggctgtt gcttctccat taattgctct aaggtctggt cttgcgtaat
     7981 atagattcct tgctgtgctg tatttttagt aataggctgc gcaagtagtt ctttttgcaa
     8041 atgatggatc gtgacatgga tttcaggctg ttcacgctgt tcagcatatg cttttgctgc
     8101 tgaaactttg acgatatttt tcagctctcc tatgtttccg gataatttct gacttgttaa
     8161 aagtgacaaa gctggtccac tgactgacaa agggctcccc atttttcttt gttcttcgag
     8221 aaaaaaagag taaatcagtt ctagccgttc attacgacta cgctgattaa gtgcaggtag
     8281 ttcgattcgg ataggtattc ttcgaatgaa agttgttaag aaatgactag taatctcttc
     8341 agtcgtagca aaaaagagac gtgttttgat ccggcgtgac aaattaggtt ctcccatacg
     8401 ataaatcaca ccttgatcaa gataggtgaa cagtttttcc tgaccttctg gtgttaagcg
     8461 atgtacctcg tctaaaaaca agatcccacc atctgctgct tcaaaagccc cttttttatt
     8521 ttcttctgca ccagtgtacg ctcccttaac atgtccaaac aaattgctag tcaaaagttc
     8581 aggattatct gcatattgtg cacaattaaa agtgatgaat ggtgcagaat cttctagcag
     8641 atcatgaagc agacaatact gatagaccaa attaactaaa taacttttac ctgtgccact
     8701 ttcgcctgta atcaaaagtg gtaatccgcc atctggatag taaagagctg ttttgatttg
     8761 ttcgatactt tctttcaaac tggcgtcgta tccgatcata aaagagaata agtcttttgg
     8821 ttgcgcgaaa aaaggctgtt cctctttgag atcatcgata gaaaaataaa aattcgtgcg
     8881 taacttaaaa aattgttttt caaaagcttc tttgtggaaa taaatcactg gtcgagaatt
     8941 gattttgact agtagttgct catcatttaa ctggttcaaa taatgactga ctgtgtttct
     9001 ttttacccca aaatgatcag ctaacctttg tgctgtaaag acctcgttca tatgttctaa
     9061 atcgaaaaag gctgtctggt tctctaagta acataaaatt tccttcttca acaacttatt
     9121 cacccctcct ccatcataga aaacggattc attttgtcaa taaaatcttt aataaagaat
     9181 tgaaaaatat atcttattat agacagcagc ctacttatcc cttataattt ttattctaaa
     9241 aaacgtacag ttccttccag tttttatact ggaatttcaa catatgaaag aataaggagg
     9301 ctatgactat tcaatgaaat cggataaata taaaaacagg aaacatactt cctggaaaac
     9361 ttttcgagaa atcatcggat cttatctcgc tgtagttgta tttttgcctt tgctttatat
     9421 ggctatgaca gaacaaactc gttttattgg cgtagatacg caatatatag ccgaaacagg
     9481 atttttattc atctttatct tgatttttgc gttctataaa tccttccata caaaataaga
     9541 ttcacagatt tcaactttcc tgtctgtttt tagctattac aaatccttat atgtggtata
     9601 acaacaattt attggcataa tgaggaaaaa cgagttatga tgctttcaga gagtaaagag
     9661 gtactgttgt caccatcctt tttattgatt acgtacacca ctcttagttt tttcccattg
     9721 attacgaaaa caaggtagaa acattgatgt tcctaccttg ttttcttttt ttattttcag
     9781 atcaaaatct caagtagctt ttgacttgat gtcgggaggt tgtcttcgtt ccatcaaacc
     9841 tgatagaatc tgcgccaaac catagttttc tccactatta tttattcctc taagatagta
     9901 cccacaataa acgaataaat atttttctca ttttcatact ccttctttga aaatcaatta
     9961 gatttctctc aacccacttt tttacaaaaa ttacgtagca aaaaagattg caagaacaaa
    10021 ttaaaaaaga agtaaataga gtttcatcta taaacattta cttgatatat tttttatgca
    10081 gaattcattt atagaaaaat taagtaaaat agacgttcct atgaatgaag agactggcat
    10141 gttttctcat tttctttttt ttaaaaaaaa tagtctattc aatttctaaa gcttcttcat
    10201 aataaaactt tcatttatga aaaggcttgt tctcttttca ttctaaattt cctaagctac
    10261 aatgtggtag tacttgtttt ttcaattgtg gacaaataaa taataggagg aatcatatga
    10321 ttcagttatt acgttatgca aaagactatc gtaagcaaat cattttgggc ccgttcttca
    10381 aatttcttga agctgtcttc gaacttgtcc tgcctttaat gatggcttcc ttgatcgata
    10441 atggactaaa aatgaatgac cgaggaaaaa tcattgaaat gggcttatgg atggtcgcta
    10501 tgtctgtgat tggtctgatt tgtgctatca tttgccaata ctatgcttca gtagcttcac
    10561 aaggatttgg tacagagtta cgtaatcagc taatcaaaaa aatcaatact ttctctcaca
    10621 acgagttgaa tcattttgga acagatacct tgatcacccg aatgacaaac gacatcaatc
    10681 agcttcagct agctttagca atggtcattc gtttattgat cagagcaccg tttttaagta
    10741 tcgggtcagt ggtcatggct tttgtgatcg actgggaagt aggtctgttt ttcttagcat
    10801 tattgcctat tttctcaatc attttatttt ttatcatcaa aaaaaccgtt cctctctacc
    10861 aaaaagtgca agaaaaacta gatcagctca atgaacacgt tagtcagaat cttagcggtg
    10921 ttcgtgtcat ccgcgcattt gcgcagacaa aaaaagaaac agagaagttc gataaaagca
    10981 caaatgatct agcgaacaat tatttacgtg tttccaatat ctctgcccta ttatcacctg
    11041 ctacaacctt gatcatgaat gtcgggatca tctgtttgct gacagttggc gggatcaaag
    11101 taaatattgg tagcttgcaa caaggacaag ttcttgcttt gatcaattat atgaatcaaa
    11161 tgcttttggc actgatcgtt gtttcaaacc tcgtcgtgat cttcacacgt gccgaagctt
    11221 caggaaatcg ggtgaaagag gtgctagaca cagaaaattc gattcttgaa gctgaggtca
    11281 gtactactcc cgatttttct tctgaagcaa ttatcgcttt tgaccatgtt gacttccgtt
    11341 atactcctga ttctggtctt tctctccaaa atattacatt tcagctgaag cgaaatacag
    11401 tccttggaat cattggtcca accggaagcg ggaaaacgac tttgacacaa ttgattccgc
    11461 gtttctatga tgtcagttcc ggtacagtta taatggacgg aatggatgtt cgttcttggc
    11521 agctggatca tctccgtcag caaattgctc ttgttcccca aacttctgta ctatttaccg
    11581 ggactattcg tgaaaacctt caatggggta agaaagacgc cacagaaaaa gaatgttggg
    11641 aagctctgaa aatcgctcag gcagaagaat ttgtccgtca gcttccaaat ggtttagata
    11701 caaaagtcat ggaaaatggg aaaaatttct ctggcggaca aaaacagcga ctgacgattg
    11761 caagagcttt gattgccaag ccagcattac tgattttaga tgactcttta agtgcgttgg
    11821 attatcaaac agacttggat ttgcgtaagg cgctacagac ttatcttggc agtacagtga
    11881 ttattatctc ccaacgtgtc cgctctattc aagaagcaca tcatatctta gtgatggatc
    11941 aaggtaagat tgctgctcag ggtacccatg aagaattact tgctcaatca gcagaatatc
    12001 aggaaatcgt cgcatcacag gaggaagaat agttatgaaa gtaaaaaaat tatccttgca
    12061 gacgctccgc cgattcattc cttaccttac tgcctatgca aaagacattt ggctagctgt
    12121 gatactagga atcatcaatg ggattgcaac agttgtgatg acgttacaaa ttggtcaaag
    12181 tatcgatcaa atgattggag aaggacaggt ccgcttcaat cgactgttcc atttacttat
    12241 tctattcggt ggtatcgttt taatcaacgt agtcagtcaa tggctcatcc aagtgttaag
    12301 caatcggtta gcttacttat ccgttgcaaa attaagaaaa gatgcatttg ctcatctgaa
    12361 tcgattgcca cttcgttatt atgatcaaaa ctcccacgga aatatcgtca gtcgatttac
    12421 aaatgatatg gacaatattt ccattgcaat ttctgcggcc ttcaatcaat tattttctgg
    12481 agtggcaatc atcattcttt cgtttatttt tatgatgcgt ctaagtccac ttctgacatt
    12541 ggtcgttctt tgttctacac caattatttt ccttgtgagt tggatcgttg ctcgtgcttc
    12601 acaaacaaat ttcgatagac agcaaaggat tattggggat atctcgggct ttgtgaccga
    12661 acgaatcggt aaccagaaaa tcgtgaaagc ttttcagcaa gaagaagcta gtcaacagca
    12721 atttgaactg ctaaatgctg atctatatga aaaaggacaa cgggcacaat tttcttcttc
    12781 cttgacaaac ccgtcttctc gttttgtaga tcaccttgct tatttatcga ttggtttagt
    12841 tggcggccta ttggcattga agagtggttc gtcaatcact gttggtgtga tttccagctt
    12901 tacgatctac tcaagtcagt tttctaaacc atttatcgaa ctttctggaa tcacgacaca
    12961 actgcaaacc gctttttctg gtctagaacg agcatttgat ttgatcgatc agccagaaga
    13021 aaagcctgat ttgccagatg cctatgcctt ggaacccaaa aatatcaaag gaaaagtctc
    13081 ttttgatcat gtttcttttt cttatgaccc caagcagcgt ttgatcgaag atttcaattt
    13141 tactgcttat ccaggtcaaa ccattgctat agtaggaaaa acaggggccg gaaaatccac
    13201 acttgttaat ttgctgatgc gtttttatga cacaacaaaa ggaacaatta cagtcgatga
    13261 ccatgacatc cgctctatcc aacgtgatac attacgtaaa agttttggaa tggtgttaca
    13321 agatacttgg ctatttgaca gtacattgcg agaaaatctt cgttttggga atccggaagc
    13381 aagtgacgca gaaatcaatg atgccctaaa agcctcttat atgtatgaat ttgtagaacg
    13441 tttgccggaa aaattagata ctaagattgg cggtcaaggg ttaaagatat cagatggaca
    13501 acgccaactg ttgacgattg ctcgaacgat gatcagtaat ccccctatgt tgatcttaga
    13561 tgaagctacc agctcagtcg atacattgac agagaaaaaa atccaacaag ccttcttagc
    13621 aatgatggaa ggaaaaacca gttttgtgat tgcccatcgc ctgtccacaa tcaaaaatgc
    13681 ggaccagata ttagtgatgg atcagggagc tattgtcgaa atgggcaccc atgatgagct
    13741 gcttcaaaaa gatggttatt accgacggct ctatcaagca caattcacag cacaataaaa
    13801 acaagcagtc ttctgggatg ggaagactgc ttgtttttta tttagcgtgg atcgctaaac
    13861 ttcttaaaat ttttttgtaa ggaatactac gtcctacgta ataaatggca attgattgaa
    13921 tcggtaacca tattactgtt tttaacaggc ggggcgccca aacgacccag ctgaacaatg
    13981 gcacatggta catcaacgca agccataatg gtgtcaaaaa taagttgatg atcagcgtga
    14041 cactaagcgt agccagaatg gcatttttcc aagtgatctc ttttcgatag aaaaagaatc
    14101 cgtagattgc tccttcaata aatgcattca atgtaaatcc aataaagaat gcagatttcg
    14161 agaagagggc cattcctact aaatcagcta aaactgcgcc tatccctgac caaaaaggac
    14221 caaaaagaat gcccatgatc acttgtggaa taaatgtgac actgatttta ataaatgggg
    14281 tctcgattgc taccagtcgg gacaaagtga ccatcaatgc aatcaataag cccataatag
    14341 caatcatacg tgcgtctaac cggtgtttct tcatttttag catctccttt aagagctgct
    14401 acatcgtatc atgcagcgaa tgcgaaaata aaaccgcgaa cattacgtcc ttcgtccaag
    14461 aggcaacatc ccatcctctt agcacttaac gctttatttt ctactctgta ttatttttgg
    14521 gatcttttcc cacagaaact atagcatatt ttcacattta ctgcaaatac gaaaaactaa
    14581 tattattgtt tatattatcc aatacgttcg tattatatcg catattatca gtttgtttag
    14641 cttttatagc tacatatagg cgaacattaa acgttatttt tccatacgct tatccatcaa
    14701 aatgaaagtg gatgcttgtc tatttcatgg acgattcttc gaaaatctag gaagccggct
    14761 tccagtcctc ttagtagcac ctcagctgtt ccgatattcg tagctaacgg aatctcatag
    14821 acatcagata aacggataag tgctgttaca tctggttcat ggggctgtgc agccagcggg
    14881 tctcgaagaa aaatcaccaa atctaactga tcctccgaaa tcatggctcc aatttgctga
    14941 tccccgccta acggtccgga tttaaaccga tggatcggca agccggttgc ttccgtgatc
    15001 ctctgcccag tcgtcccggt agcaaaaagt tcatgttttt ccaatattgt tttatacgct
    15061 gttgctaatt tcaccatcat aggttttttt cggtcatgag cgatcaaagc gattttcata
    15121 agagacactc cttttgatat tgttaacata cttatttact ccagtgtatc agtttttatt
    15181 tcttatggat atttattaaa gcttaaaact agcacattga caaaaaaaag aaaaaattct
    15241 tgttcaacat gacattgaag ttagttaacg ctttcattta tactttaaat agttaataaa
    15301 actaaaaaag aaagtgtgga acgtattatg gtagaaatgg ctctgaaaaa cgtatataaa
    15361 aaatatgaca atgcagaaaa ttattctgta actgatttta atctatcaat caaagaccgt
    15421 gaatttatcg tttttgttgg tccttccggt tgtggaaagt caactacttt gcgaatgatc
    15481 gcgggactgg aagatatctc tgaaggagaa ctaaatatcg gcggaaaagt catgaatgac
    15541 gtggcaccta aagaccgtga tattgccatg gttttccaaa actacgcatt gtatcctcat
    15601 atgactgttt ttgataatat ggcttttggc ttgaaattac gtaaatatga taaagcagac
    15661 atcaaaaaac gtgtggacaa tgcagcagag atcttaggtc tgacagaata cttgaaacga
    15721 aaaccagctg ctttatctgg tggacaacgc caacgtgtgg cattgggacg ggcaatcgtt
    15781 cgggatgcaa aagtcttctt aatggatgag ccattatcaa atttagatgc taaattacgt
    15841 gtagccatgc gtgctgagat tgccaaactt caccagcgtc ttgaaacaac gaccatctat
    15901 gttactcacg atcagacaga agcaatgact atggctgacc gaatcgttat catgaaagac
    15961 ggatttatcc aacaaatcgg tacaccgaaa gaagtttatg atacacccgt caatatgttc
    16021 gttgccggct ttattggatc tcctgccatg aacttcttta atgtgacatt gaaaaatggt
    16081 gttatcacag atggacatgg gcttaagctt cgtattcctg aaggaaaaaa caaagtatta
    16141 gttgaaaaag gttatgaagg aaaagaagtt atcttcggta ttcgtccaga agacatccac
    16201 agtgaacaag tagcccttga agcttcacct gaagccgtag tgaaatcaga agttgtcgtt
    16261 tccgaattac ttggagctga aacgatgctt tacacaaaaa ctggttctac tgagttcatc
    16321 tcaaaagtag atgcaagaga tttccataaa ccaggcgaaa tagtcgacct tgccttcaac
    16381 atcagcaaag gccacttctt tgataaagaa acagaacaag tcatcaaact tccataaatt
    16441 gccccctgtt agagctgtgc cagaagatgt tggtgcagct ctttttagga caaaaaactc
    16501 tcatcttaga ggatttatta tccattacta gatgagagtt ttttttttga gagatcatgt
    16561 ctaatatgtc gttttcttcg cgtacccttg aatcgactct ttccgatttt ctttttttgc
    16621 tgccgtaaat acggggatat caaaatattt gctgagtgtt cctgctataa aataagcaat
    16681 attataatca actaaactgt ggataagccc accaatcccc atcaagatga atactgtata
    16741 aaaatatcct tgagaataat atgtttcagg catattacca ataaagtaaa agatgctgac
    16801 tactagcatt tctgctaaag aatgaatcac accgatcaca aaattgaacc cttggaaacg
    16861 ccaattagca aaagtaaact gtccattttt taaaacgata ctcggatgct tttgcaagta
    16921 ccaagcccca atcaacgcaa acacaagatg tgacaatgct cgcaatgcga tgataaccgg
    16981 cgttgttaaa aagaaaccaa atgtcgtacc tagcgcaaca gccactgcaa ctcctggtgt
    17041 aaaaaacatc gcaataaata atggcacatg agacgccaaa gtaaaggaag ctggtggaat
    17101 catgatttta ggcatgacca ttgggataat gacccccatc ccaatcaata aagcagaaat
    17161 cgttagtttt cgaactgaat tttttctcat tttttctctc tcctaatatg tcttgacaca
    17221 taaatgtcga cttagtataa ctcacttact aataactgtc aagacacata ttcttttcta
    17281 aatccataca aaatgagtgt gagtatcaag tataagaatt ttttgatcaa gaaacaaaaa
    17341 taggagatgt gacaaaattt gtgtcacatc tccttgttcg gcataatagt ggtcaaaagc
    17401 ttgcatcaag taataagacc aactaactca aaaacatgag aagctgtttt ttgaaattgt
    17461 tccttgtgac ttagagttaa acggcaggcc cacaacctct ttcttaaacg gtgttcaaaa
    17521 gcttgcatct gcggtaataa gcccaaacaa acgaaaaaca tgaggaacta tttttgtttg
    17581 gctgttctta ttacttgatg caggacagct ttttcacaac ttctgtcaca acctcttttt
    17641 ttactgcata ttttctgaca tcgcattgct taatgcggcg aattcttgta atgacaaagt
    17701 ctccccacga cgttttggat cgattccagc tgtttccaag ctttttgcta accatgcttt
    17761 tgtctggtca tctttgccat acgaatgctg caagttattc caaagcgttt ttcttctttg
    17821 ctgaaatgcc gcttttgtca atcggaaaaa tgctttttcg tctgtcactt cgactgccgg
    17881 tatatctctt cttgtcagtt tcaaaatcgc tgaatctaca tttggctgag ggacaaatac
    17941 tgtttttggt acgatgaatg ctaaactggc ttccatataa tattgaacag caatcgataa
    18001 tgaaccataa gctttcgttc ctggttcagc agagatacga tcagctactt ctttttgcat
    18061 catgacgact agttcatcca caaccaaatc tgattcaaga aaatgcatca tgatcggtgt
    18121 cgtaatatag taaggtaaat tcgctacgac ttttaacgga agctcctcat gaaatgtttc
    18181 tctaacagct gttgacaaat cggtctttaa aatatcttga tgtacaatag tcacattgtc
    18241 atagggttgc atcgtatcag ccaatacagg gatcaaacga tcatcgatct caaatgctac
    18301 gacttgtttt gcatgcttgg ctaattgttc cgtcaatgca ccgattcctg gtccaacttc
    18361 gatcacgttc gtatgttcat ctatttgcgc agtttctacg attttcctta aaatatttgg
    18421 ttcagtcaga aaattttgtc ctaaactttt tttaaatgaa aaaccatgtt tcaataaaat
    18481 ctcttttgtt ctagagggtg tcgcaatttc acgatattca ctcatgttgt tcctccacta
    18541 ttttcattgc ttgggcgagt tctttcggcg taatacgaaa catcttcaaa cgtttttcca
    18601 attgtttgcc gttcgtgtaa ccgatgcgca actcgtcacc tagtttttct cgtttttctt
    18661 ttgcttgtgc cccagctatt aaaccatagt ctaaaaggtc ttgtctagtg atttcagaaa
    18721 cagccgtttg ggaaatgtct ggcgtcacga cctttttcaa tgcttctaaa atagcagcat
    18781 cacttgcatg ctcgacacca aggcttccct ttttctttgg tttagcttgt gtgcgcggca
    18841 aaaaagcatg ttttgcctga ggaacagctt ccataatgat ttttcttatt ttttctcctg
    18901 aaaaatcagg atcggtaaaa acgatcactc cacgagtctc ttgggcatgt tcaatttgca
    18961 tcaggatctc ttcattgatt gcagacccta ttgtttctat cgtatctgca tcaactactt
    19021 cccgcagcct tcttgtatcg tcttttcctt caacgactat aatctcttgg atcttcatct
    19081 tatccatgtt cgcttagacc aaataatcgg tgcgcatttg tccatgtttg ggcagccact
    19141 tcttctgtcg gaagattccg aagttcagca atcttttcta ctgtataacg agtatagcct
    19201 ggttcatttc gctttcctct aaaaggaact ggtgccagat aaggggcatc tgtttccaca
    19261 agtaatcgtt cgattggtac atttgctgca gcctcttgga cgtctaatgc ttttttgaaa
    19321 gtgacgacac cgctaaatga aatatgcatc cctaagtcaa gaaatcgttt tgcccattca
    19381 taatcaccac tgaaactatg catgattcca ccgatatctc gaacatcttc ttcttttagg
    19441 atttgatacg tgtcttcaag tgcttctcta gtatgaatac tgattggtag gttcatttct
    19501 tttgcgatag caatctgtct gcgaaatact ttatcttgga cttctttcgg gtcttccatc
    19561 caatgataat ccaagccaat ttcccctaat gctacaacct ttggtaaagt caattgttct
    19621 tgtaatttat tttcgatttc tttcgtataa ctgccagcct ctgtcggatg ccagccgata
    19681 atactgtata tatttggata gtcatggctt aattgcaaag acttttcaat cgttggcgta
    19741 tcgaagccca cgaccgccat tttcgttacc cccatttctt gtgctcgctg aatcgtttct
    19801 ggaatatctt cattgaactg ttcagcattt aaatgggtat gagaatcaaa tatcatgttt
    19861 atctctcctt ttcattctta ctttagcaca taagcactac ttgcagaagt agcaaaaatc
    19921 aaaatagaag aacttgtttt attgaaatag aaaaagcacg aagatctaga ggacctagtt
    19981 cgtacttttt ctgtctgttg ttgaaatatt ttactcttct gagcgttttt tctttttttg
    20041 ataatagact gggataccta tcaaggtaac acctataccg atcaatgcta atttcgtttg
    20101 cgtcatcatc gtcataccta aaatgaaaat agcaccgaga atcgcaataa tcggaacgat
    20161 tggataaagt ggtactttat atggacgtgg caattccggc gctttttttc ttaaaataaa
    20221 gacaccgata aaaatcagta aactgaatag ccacatcaca aaaatcaaca tgtctgttaa
    20281 aaagtcgaaa gaaccaaggc tcatcataat acaagctact gctaactgga aaaccgcagg
    20341 aacataagga accgtaaact tctttgaaag ctttgtcagc tgtttactga aaggtagctc
    20401 gtcttctaaa gccatcgcat aaggcacgcg gatcccagtt agtgtataac cgttcaatgc
    20461 accgtatacc gagatcaaga tcccaatagt gaccagcttt ccaccgattc ctccaaaaat
    20521 gacgtctgat gcttcagaag ctgcattcaa attaccggca agatgatcaa tcggcaaagt
    20581 tttcaaaaaa acaaaattga tcaatagata aacgactgtt acaaaactca aaccaagaat
    20641 aattgcttta ggcaaatctt tttccggccg tttcatttcc cctgccattg caccgacacc
    20701 taaccagccg tcataagcaa acaacgttgc taacaacgca ctgctcaatc cttcgacaaa
    20761 agtaacatct tttccagctt cgattggaaa taactggaca gttccttgtc ctggggtcaa
    20821 taatccccat atgacaatca cagcaattgg tattaattta acaatcaacg tcactgattg
    20881 tacagaagca ccgacttttg tccctaacaa attcaaccct gtcacagaaa cagcggtgat
    20941 cactgcaatc aacaaaagat aatcggtaga taattgtaat aagttcgtca attgtgtcgc
    21001 aaagataatc gataatgcag caatattagc aggaaagtaa atcaagcttt gcgcccatcc
    21061 taataaaaag ctgggcaatt ttccatacgc agcttcaata tattttacag ctccaccagt
    21121 ttccgggatt gctgtagcta attcagccac tgtcaatcca gcacagatcg ttaagaaacc
    21181 acccaaaagc caagctagca atgtcaaact tgtagactga gtactagcag taacagcagc
    21241 agttttgaag aagacgcctg caccgatcac ggttcccata acggtagcta atgcgccaaa
    21301 cagattgatt tctcgtttta gtttgccttt cattcccatc ctctttcttc tttgaaattt
    21361 ggtataattt tcagtataac tgagaatatt ttaatttacc attctttttt ctccactttc
    21421 agtgaaaaac tccctcttaa gacggatgta tttctttttt atttttcgta tactaaacct
    21481 accttttcca cgaaggagga acagtttatg gattttaagc gttcttatcc gaaattagca
    21541 gattatttat ccaaccgggt cgatttttcc cgtaaagaac aacagcagtc ccattggcat
    21601 tggttcacat taattttatt tggattaatg actgttgcaa cgttatcaca aaaactaata
    21661 ttgctgactt tgttcatcgc aatcgttgca ttgatcaaag gtcctgtgat gatcttgtgg
    21721 gggatgttgt attccttttt ggttgggctt tttccgccta tcggtatttt gctttcagcg
    21781 attttctttt tattgaatat cggaacattt acaaagaact ggcggatgac cttagtcggg
    21841 atctattttt atttctatcc ttttggagtg atggcactta gtgaagtcat gcattgggat
    21901 aatcattggt ttatagctgg ctccttgctt ttagggttga tcttgttaca cgtgatgttg
    21961 acaaagctct atcaacatta tggtatcgga cgtacgattt tctggtatgt cttttcgatc
    22021 ccatttgcac tgcttactgc tctcttacca agccgtttga agacaaaaat aaaaggttac
    22081 cataaaatca agtgatagga tttttacaaa ataaagaaaa caatcatcga agtcaaagcc
    22141 gatgattgtt tttttatcta tattttcaat cacacatata agagcacaaa ctaattgaac
    22201 ataaagtgat agaacttata attaacacat caacgctact gttagatata aatatagatg
    22261 taataaagtc attgcattca aactttatga atgtaaggac aataaaaaag agtaccagat
    22321 gaatcaatta aaatctgccc gaccctttat cattatgctt tttttctgtt tttacgattc
    22381 ctattagtat tattctaaat taacaaaaat cagcgaagct cgtacttctt tttctaggat
    22441 ttattttgga tagttctctg tttctgtatt atatgattgg cttcaagtaa taagaaggaa
    22501 tttacgaaaa ttgcttctca aatttcggaa aaagggggta tgacacaact agtgtcatac
    22561 cccctttgaa tttctatgcg atttctgagc catttggcat ggattttggt gcttcgatta
    22621 cttgtaaaga gccatctggt gcttctgcag aaaggatcat tccttgactg atttgtccac
    22681 gcatctttcg tggtttcaga tttgctacaa tgacgacttt tttcccgatc aattcgcttg
    22741 gatcaggata aaattcagcg attcctgaca agatttgacg gtcttggctg tctccagcat
    22801 ctagtcggaa ttgcagcaac ttatctgccc cttctacttt ttgacagttg atgacttctg
    22861 ctacttttag ttctactttt tcaaatacat caaatttgat ttgtttttct ttcgttgaaa
    22921 caagttctgt ttcttctgga tcccatttga ctgtatcttc gtttgtttga gtaccttcag
    22981 acattttttc ttgaataaaa gcaacttctt cttccatatc taaacgtggg aagattggtg
    23041 taccttttgc aactactttt gttcctgaag gaaattcgcc aaagtgtaat ccttcaagat
    23101 tcattgtttc agagtccaat cctagttgat tgaagatctt ctctggcgtt tctgtcatga
    23161 ttggctgaag taatatcgct gcgatacgca aagattcagc taaatggatc atgacactat
    23221 tcaattcagc tttcttttct tcgtcttttg ctaataccca tggttctgtt tcatcaatgt
    23281 atttgtttgc acgagaaatc agcacccaga tttcagccaa tgctgtattg aattccattt
    23341 tttccattgc atcatggtaa cgtccgacaa cgttagcagc cgtcgtagac agttcactat
    23401 caaatggagt gacaagtgag gcatagtcag gaacgagtcc atcacaatat ttgttgatca
    23461 tagcaatcgt tcgattcaat aagttcccta gatcatttgc cagatcataa ttgacacggg
    23521 aaacaaaatc ttctggcgta aagacaccat ctgacccaaa cggaacagca cgaagcaaat
    23581 aataacggag tgcatctaaa ccataacgtt ccacaagcat ttcaggataa acgacatttc
    23641 cttttgattt agacattttt ccgtctttca tcaatagcca gccatgccca aatacttttt
    23701 taggtagagg caaatcaagg gccatcagca tgattggcca atagatcgta tgaaagcgaa
    23761 cgatttcttt accgaccatc tgaacatctg caggccagta tttttcgaat agactttcat
    23821 cttcggagcc atatcctagt gcagtaatat aattggataa tgcatcgatc catacataca
    23881 ccacatgttt cggatcactc ttgactggta ctccccaagt aaaagttgtt cgtgaaactg
    23941 caagatcctc taatcctggt ttgataaagt tgttgatcat ttcgttttta cgagattctg
    24001 gctggatgaa ttcgggatgt tcttcatagt attccaatag acggtctgcg tatttgctca
    24061 tgcggaagaa gtatgattct tcttttacca attctacttc gtgtccactt ggcgctttac
    24121 caccgatcac gttaccttcg tcatctttgt acacttctgc aagttgtgtt tctgtaaaga
    24181 attcttcgtc agaaacagag taccagcctt catattcacc taagtaaata tctccctgtt
    24241 ctaataaacg atcaaatatt ttttgaacag ctgctttatg gtaatcatct gtcgtgcgaa
    24301 tgaatttatc ataactgata tcaagcgttt tccataattt cttgacatca gccgccattt
    24361 tatctacata ttcttgcggt tttacaccta gttcttctgc ttttgtttcg attttttgac
    24421 catgttcatc cactccagtg agataaaaca catcaaagcc catcaaccgt ttataacgag
    24481 caattgcgtc acatgcaatc gtagtatacg agttgccgat atgaagtttg ccgcttggat
    24541 aatagattgg cgtagtgata taaaaagttt ccttttctgc cacggggctc ttcctccttt
    24601 atcgcatgat gcgaattcgt ttccttgcta gtataccata aaaaaagcat gctgttagct
    24661 ttttttcaaa atgaaaaaga ctggataaac gacttctatc tacgttcaat ctatccttta
    24721 aaaggatgaa taggttgaat aaatgcgggt ttttgttttg tttcatatct actaaaaaac
    24781 ggctttttat ttctcccctc acacgtggta taataaagac tatctatctt agtaagggac
    24841 tggaacaatc atgcagaaga aacatctttt ttttacactt agtattgctt ttctttcatt
    24901 agcacattta atattcagct atttttatat tcgtatgtat ggttatttta acctccatgg
    24961 gcatttgaac agttttatga cagcagcttg gattttgcgt tttatcattg acgtatatat
    25021 cgttatttgt ggcttctttg ccattcgtga agagcggtat aaagtattgc cgttttatct
    25081 gcttttcttt ttattcaatc tcatacttcc atttattttt catatttaaa tgataaccta
    25141 gatttttcaa gtaaatcctt ggtttttttt cagagtctgc tacaataagg taaaaagtac
    25201 ttttactgtc aatggaggtg gtcagatgaa ccgttttgct tctaaagaag aagaaaaatt
    25261 atattatgaa cataaagcaa gtgaagaaga atttcttaac tggtatcaaa aacaggaacg
    25321 tccagaatat gacaagccct ctttgactgt cgatatcgta ctgatgtgct ataacaaaga
    25381 agaagatcag ctgaaagttt tgttgatcca aagaaaaggg catccttatc gaaattcatg
    25441 ggcattgcct ggtggctttg tccaaaagga tgagtcaact ggagaaagcg tcttaagaga
    25501 aacaaaggaa gaaactggtg tggttatttc aaaagaaaat attgagcaac tgcatacatt
    25561 cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca gtcagctact tagcctttat
    25621 tggagaagaa ccgctaatcg ccggagatga tgccaaggaa gtacgctggt tcacactaga
    25681 acgtcatagt aacaagatcc atttatctag cggagaagtg gctatctcgc ttgatttagt
    25741 cactggagag tcttctggca aagatacttt ggcttttgac catagtcaaa tcatcttgaa
    25801 agcattcaac cgtgtagtca ataaaatgga acacgagccg caagttctac aagttttagg
    25861 taaagatttc acgattacgg aagctcgaaa agtatttgct aaattcttag ggatcgatta
    25921 taaaacgatc gatcattcca attttaagaa agctatgctc caatatttcg atgagatcgg
    25981 tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat cagctcaagc cggggcacca
    26041 tgaataaggt tgtgagcaaa agattcagac ctaatcattc aagaaaaaac gcaaaaacaa
    26101 ctctttgtga gttacgtttt ttgtttcaga attttgatgt attcgtaaac tttctgaata
    26161 aaaaattaac aatagcagaa aaagctcgta cgaacttttt ctgctattgt tttattggtt
    26221 tgtcataatt agtgagcttt tcttcaaaag aaaaattcct agaaacaaaa gtcattaaga
    26281 ctttttatta acaaggctaa ccctaacgct cctatcagtt tcattcattt tttatccctt
    26341 gataaccgaa tgtctgccca ttttttctac tacttggctc tttcttacgg aattgctttc
    26401 ttctgctccg tcggtattga tcaaaagaaa ttttttatta taccattggg acttataacg
    26461 caattttgtt attaatttat gccagtcgtg aatagaaaaa tcagcatgtg cctcttcttt
    26521 tggaaaagat tctacaaata agtagtcata ttgtttgacc aatttcccag ataattgttc
    26581 aagataatct tctcgctggt tcttttgttt cagataaaga tccattactt ttctcttttg
    26641 tttctgataa ttgcttgcct gatctaacct gatctttcta tggtgagcgg agcggacttt
    26701 cacattcaat ttccgctgtg catgttgcag cttttcttct gtctggtgaa attttggtaa
    26761 agttacttct agaggtcgag atgtttggat caatgtcttc ggatgatagg cgatcccgat
    26821 ccatttagct aaagattctt tttttactgg cgaaacatca caaagtagtg cgacataaaa
    26881 ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc gatccttcaa ctggccgatg
    26941 ctgatttatc ttgattagtt ctttttgttt tggtaacttt aagtagccat tttccagata
    27001 gatcgtacgg gtttggttgt tggtcgtata agattgccac atactttttt tcgttttaag
    27061 ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa gcccgatcca aattaagctg
    27121 tgcatttgct aaagcaagac tgtctacttc ttttaagaat gggtattctt tttttaatgt
    27181 agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc caacttttct gccgcttttt
    27241 cagcaaatgg ttataggtaa atcgagcaca tccaaaagta cgaatcaatt gctgtttttg
    27301 ttcttctgtc ggatacatac ggaatttaaa agcttttagc atctatgtcc ctcctagtta
    27361 gcaaccagaa gagattcctt ttgtcgtttt agtttttttg cgtaatatgg gacttcctgc
    27421 tcagttgcat agacaaaatg ttctggtgcg atttcacgaa gttgacgtac ttgtccgtca
    27481 tctggttcag gttgatattt gattcttctg cgttttctct catggtctgg atctggtaga
    27541 ggaatagcag ataacaaact ttctgtataa ggatgaacac cgtgattata gatttcatca
    27601 cttgttccca tctctaataa caagccgtta tgcattacac cgattcggtc actaatatgt
    27661 ttaaccatcg acaaatcatg tgcaataaat agataggtca attgatgttc tttttggaga
    27721 tcttgcaaca aattgaccac ttgggcttgg atcgacacat ctagtgcaga aattggttca
    27781 tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg caataccaat acgctggcgt
    27841 tgcccacctg aaaattcatg aggataacga gtaccgtggc ttggattcaa tccaacagtt
    27901 ctcaatagat catcgacttt ttttgcacgc tcttcagatg atttagctag tccattaaca
    27961 tcaattcctt cggcaatgat atctctgact ttcatacgag gattcaacga agcgtatgga
    28021 tcttgaaaga tcatctgaac atcatgccgg aatttcgtca aacctttttt atctttgatc
    28081 ttcgttatat cttgaccgtc gaataatatc tcaccatcag taggttcatt caagcgaatg
    28141 atcgtacggc cagtagtgga ttttccgctt ccagattctc caactaaacc gaatgtttct
    28201 ccttcataaa tgtgaaagct gatgtcattt acagcatgta cttcattctt ttttcctaca
    28261 ttgaaatact gtttcaatcc tttaacttca agtagtttat ttggcatcag caattcctcc
    28321 ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc cagcgacgtt ggatttcttc
    28381 tggcggtgtc actttgggtg cctgcggagc aagtagccaa gttgctgcct tgtgtgtttt
    28441 tgatagttca aaaaatggtg gttcttgttc tgcatcgatt ttcatcgcga attcatttct
    28501 tggatagaat gcgtctcctt ttggcgggtt caataaatcc ggtggagaac caggaatagc
    28561 atacaatcga tcgttcgcac tatctaatgt cggcattgaa ccaagcagtc cccaagtata
    28621 gggatgttgc gggttgtaga aaatttcttc cgatgttcca acttcaacga ttcttcctcc
    28681 atacatgaca gcgacacgat cagcgacatt cgctactaca ccaaggtcat gagtgataaa
    28741 gataattgat gtatcgattt ttgtctgaat ttctttcagc agctccaaaa tctgtgcttg
    28801 aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc aggatttccg gataacatac
    28861 cagtgcaatc gcgatcacga tccgttgacg ctgacctcct gagaactgat gcggataact
    28921 gttgatacgt ttctcagctt cgggtattcc aactaattta agcaattcta atgcagcttt
    28981 ttgtccctct ttttttgaaa ctttattatg ctttctaagg gactcagcga cttgcttccc
    29041 aatgggcatc gtcggatcta aagaggtcat tggatcttgg aaaatcatcg ctatttcttt
    29101 accacggatt ttttgcatct ctctttctga ttttccaacg atatcgttac ctttaaagag
    29161 gatttgacca gcatctatat tggcgttgct tgacagcaag cgcatgatgc ttctggtcgt
    29221 cactgatttt ccacttccag attccccaac gatcgccaat gtttctcctt tattcaaatg
    29281 aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac gtatcaaaag agatttctaa
    29341 gtctttcact tctaaaattt tcgtcatcgt cttcactctt tcattttagg atcaaaggca
    29401 tcacgcaatc catcagcaag taagttaaag ccaatcatga taaccgataa agttactgcc
    29461 ggaatccata atagataagg cagatacagg aatgtcttgt acccttcgct caacatcgtt
    29521 cctaatgaag ctgacggagg cgtaagacct aaaccgataa agcttaaaaa ggcttcgaag
    29581 aaaatagcag atggaatact aaacatcatt tgaataataa tgacactaga gatattcggc
    29641 aaaatatgtt tgaatgcaat tttcattctt gattcaccta atgtcaatcc agctaaaaca
    29701 tattcttgat cttttaattt cattgtttgt gcacgaacga tacgtgccat tggtatccag
    29761 tttgtgatgg ccatagcagc cacgattgaa aaaattcctg gttcaaaaac gaccaacatc
    29821 aaaatcatga caacgaggtt tggaattcct gataagattt ccaaaaaacg ttgcatcacg
    29881 ttatcgacac gtccgcctaa caaaccagaa accaacccgt aagctacacc aatcgttaca
    29941 tctagtaatg cagcaataaa tgcgatcagc aacgagattc tcattcccat aaataagcgg
    30001 cttaatacat cacgtcccaa accgtctgtt cctaaaaaga aatttacgtt tgaagggacg
    30061 tttgcttgag catatttatc aacaagttca ccaccaacca ttgcttttcc attcaatcca
    30121 tcgatattga ttcccggaat acgaggaggc aaattgatat aagccacgtt ttgggcagtt
    30181 ggatcatgag gagaaacaaa gattgtgatc acagagatca aaatgatgac caatagtaaa
    30241 accattgaaa taacggcagc tttgtttttc tttaaacggc gccatgaatc ttggataaaa
    30301 ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg tatttttttg taatggaaga
    30361 aattcatccg cagggatatc tgcaactgtt tcataacgct ttggtatcat ttccatttat
    30421 cctcggctcc cttctgaaac acgaattctt ggatcaacta gaccgtacaa tagatccacg
    30481 atcaaaataa caaaaatcag cataaatgag taaaggatcg ttacagccat gatcgtagga
    30541 taatcatttg ttgtgattga cttaacgaat tgttccccga taccaggaat agcaaagata
    30601 ttttctacca ccagtgatcc ggtcatcaat gctacagcta acggtcctaa taaagtcatc
    30661 aatggaatca aactatttcg aagaccatgt ttaaaggcga tttcccaacg gctcaatcct
    30721 tttgcttttg ctaattccac ataatcacta tgcagtactt ccaccatttc cgtacggata
    30781 aaccgagcag agtcagcaag cggcgacatg gccaacgcaa ttgttggtaa gattgtgtac
    30841 acaaagccat cccattttgc gattggcaaa acttgtaatt tgattgcaaa gatatattgt
    30901 aataaaacag caaatacaaa gtttggaatg gaacgaccta atatcgctac gagtgtagaa
    30961 gatgtatctg cccatgtatt ttgcttcatg gcagagatcg ttcctaaaat cgtccctagg
    31021 actgttccaa agataattgc ctgtaaccct aactgcaagg atggtccgat tcttccagct
    31081 aacaaatgtg cgactggctg gtttttaaat tggaaggaaa ttccaaaatc tccttgaagc
    31141 aaattagaaa gataaatccc atattgtacg ataactggtt tatccaaacc gacttgttta
    31201 tttagcatct cgattgtttc aggactcaaa cgttcctgat tcgtatatgg cgtgcccggc
    31261 agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc aaagcgtgat gatcatgaaa
    31321 aagacacgtt ttaatacata tttgatataa ctattcacta atgtttcccc ctagcttttg
    31381 acttctgaca gatgtttcgc tacattatta gataatgaac caaaaataaa ggagcaatca
    31441 aatgaaaaaa aaatctatcc cctacgcagt agcttttcta ctgattctgg ttattttaat
    31501 aaaaaatctc atcaaccatt cctttacact cattcaatta tctaatgatc tattcttatg
    31561 gtcattgccg tttctgatta tcggcggttt tttatgggtc ttttcttcgg gtttcttcga
    31621 tcattttcag cgatcggtcc atcttgcacg aacaaggaac cgaaagaaaa aaccagaatt
    31681 ttcctcgctc tcgtctgcca gctacggtat gtacagcttt tggctgatca ttgccggaat
    31741 actaattgct ctttctgcta ttttcatgct tttttctttg ttaggataag tgaatatgag
    31801 aacgaggttg cgacatttgc cgcagcctcg ttttttctca tgctcacggt attaacgatt
    31861 attcttcaac gtatgtccat ttgtaatccc atgaagcgcc tgccgcatgg tgaacgatac
    31921 ctttgatttt ttcattcact aggtgacctt ctgctttttg gtacaccgga acgacaccca
    31981 tatcatcaga gatgatcttg attgcttttt ggaaatcatc ccaacgggct tgttcatcgc
    32041 cagcgtctgt tgatgaagct gctttaactg cttcgtcata ttctgcattg ctccagcgac
    32101 cacggttgta tgaattacct gtgacaaaca aatcagtaaa gctgcttggg tctgcatagt
    32161 cagcagccca tccgcctagt actgcgtcaa aatcacccga agttgagcgg tcaagacgta
    32221 cagagaatgg aacaggtgtt ggtttgactg ttacgccatc caagttttct tggattgagt
    32281 tttgaacata ttcgattact tttttcgatg aatcatcatc agaagccatc aaatcaaatt
    32341 ctaaagaatc aacgcccaat tcttttttcg ctttttccca atattctttt gctttatctt
    32401 tgtcataaga aactaatttt ccagcttctt ttgcaaagtc ttcgttcgtt gttggacttt
    32461 ttaccatatc tgatgggatc aatcctgtag agacaactga tccgtcacct agtacttgtt
    32521 ttactaaagc ttcacgatcg atagaataag aaatagcttt tcttaggttc acattgttga
    32581 atggtgaatt cttttcacgt tgattgaatt cgatatagct tgtccgcgct tctttaacag
    32641 aagtataagc tggatcgttt gcgttttgct gtgctaactc accagacaag atcacgtcat
    32701 ctgcttggcc atctttgaat aagttcaatg cagtagaaga ttcttttact acactgactt
    32761 tgatttcaga aagttttact gtatctttgt cccagtaatg atcatttttc ttataagtcc
    32821 attctgtatc tgttcctggt ccatcaaaat cttctaatgt gaatggtcca ttataaacag
    32881 ctttatcact tgcagatgca taggcatccc cattttcttc cactacggat tgtttttgtg
    32941 ggaaaaatga tgggaaagca agcagatatt ggaaatatgg tgtttgtttc gtcaatttga
    33001 tttctaattc atagtcacca actgctttga tccctagttc tgatttatct ttctttccag
    33061 ctgtaatgtc ttctgcattt tcaactggcg catacaggta agcatattca gaagctgtag
    33121 ctgggtctac tgttcgttgc cagccataaa cgtaatccgc tgctgtaaca ggatcaccat
    33181 ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt taatccgtct tcactgactt
    33241 ctgccaactc gctagctcct gccggctgtg gtttgctgtc ttcatcaaga cggtaaattc
    33301 cttcataaac gttattcaat gctgtaaaac tgattgtatc tgttgcaact gataagtcag
    33361 ctgtaggcat ttcttgaggc acgactaagt taaatacgcc tcctccatct gctgaaccgc
    33421 tttttgctga actactgctt gtggctgaac tgccgccata acatccagca agtacaaggc
    33481 cgcataccgc aaccaaacca aatgtaaatt tctttttcat tttacgtacc cctttattgg
    33541 aattttcaga ataattaata ttttctgaag ttgtttaaga gtttaatgaa taattattaa
    33601 aaaatcaaca cttttttttg tctttttcat aaaataatga ttttttcgtg aggtttcaag
    33661 gaatttttca gaaaaaatag ctttgtacaa ggttttccat ggtcttccaa aagccattaa
    33721 ataattttcg ttatattgtc tattattgcc atacaaagat aaaaaagaat aaacaaaaag
    33781 aaactgtgac aaaagtcttg tcacagtccc ttatctga
//
LOCUS       DACCVU010000021        33676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCVU010000021 DACCVU010000000
VERSION     DACCVU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.021"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            494..1201
                     /locus_tag="IVW29_000844"
     CDS             494..1201
                     /locus_tag="IVW29_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7838344.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1260..1904)
                     /locus_tag="IVW29_000845"
     CDS             complement(1260..1904)
                     /locus_tag="IVW29_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7838345.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2031..2318
                     /locus_tag="IVW29_000846"
     CDS             2031..2318
                     /locus_tag="IVW29_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838346.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2426..2746
                     /locus_tag="IVW29_000847"
     CDS             2426..2746
                     /locus_tag="IVW29_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7838347.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2827..4140
                     /locus_tag="IVW29_000848"
     CDS             2827..4140
                     /locus_tag="IVW29_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7838348.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4137..4856
                     /locus_tag="IVW29_000849"
     CDS             4137..4856
                     /locus_tag="IVW29_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7838349.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4984..5535
                     /gene="raiA"
                     /locus_tag="IVW29_000850"
     CDS             4984..5535
                     /gene="raiA"
                     /locus_tag="IVW29_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7838350.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5757..8291
                     /gene="secA"
                     /locus_tag="IVW29_000851"
     CDS             5757..8291
                     /gene="secA"
                     /locus_tag="IVW29_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7838351.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8417..9518
                     /gene="prfB"
                     /locus_tag="IVW29_000852"
     CDS             join(8417..8479,8481..9518)
                     /gene="prfB"
                     /locus_tag="IVW29_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7838352.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9623..10309
                     /gene="ftsE"
                     /locus_tag="IVW29_000853"
     CDS             9623..10309
                     /gene="ftsE"
                     /locus_tag="IVW29_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7838353.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10293..11186
                     /locus_tag="IVW29_000854"
     CDS             10293..11186
                     /locus_tag="IVW29_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838354.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11338..12786
                     /gene="cls"
                     /locus_tag="IVW29_000855"
     CDS             11338..12786
                     /gene="cls"
                     /locus_tag="IVW29_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7838355.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13123..13980
                     /locus_tag="IVW29_000856"
     CDS             13123..13980
                     /locus_tag="IVW29_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7838356.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14095..15015
                     /gene="pstC"
                     /locus_tag="IVW29_000857"
     CDS             14095..15015
                     /gene="pstC"
                     /locus_tag="IVW29_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7838357.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15015..15899
                     /gene="pstA"
                     /locus_tag="IVW29_000858"
     CDS             15015..15899
                     /gene="pstA"
                     /locus_tag="IVW29_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7838358.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15914..16720
                     /gene="pstB"
                     /locus_tag="IVW29_000859"
     CDS             15914..16720
                     /gene="pstB"
                     /locus_tag="IVW29_000859"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7838359.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16736..17494
                     /locus_tag="IVW29_000860"
     CDS             16736..17494
                     /locus_tag="IVW29_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7838360.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17507..18184
                     /gene="phoU"
                     /locus_tag="IVW29_000861"
     CDS             17507..18184
                     /gene="phoU"
                     /locus_tag="IVW29_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7838361.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18407..19438
                     /locus_tag="IVW29_000862"
     CDS             18407..19438
                     /locus_tag="IVW29_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7838362.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19435..20202
                     /locus_tag="IVW29_000863"
     CDS             19435..20202
                     /locus_tag="IVW29_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7838363.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20302..21870
                     /gene="liaX"
                     /locus_tag="IVW29_000864"
     CDS             20302..21870
                     /gene="liaX"
                     /locus_tag="IVW29_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7838364.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22170..22487
                     /locus_tag="IVW29_000865"
     CDS             22170..22487
                     /locus_tag="IVW29_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7838365.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22487..22837
                     /locus_tag="IVW29_000866"
     CDS             22487..22837
                     /locus_tag="IVW29_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7838366.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23004..23942
                     /locus_tag="IVW29_000867"
     CDS             23004..23942
                     /locus_tag="IVW29_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7838367.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23957..24787
                     /locus_tag="IVW29_000868"
     CDS             23957..24787
                     /locus_tag="IVW29_000868"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7838368.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24831..25856
                     /locus_tag="IVW29_000869"
     CDS             24831..25856
                     /locus_tag="IVW29_000869"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7838369.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25871..26809
                     /gene="galU"
                     /locus_tag="IVW29_000870"
     CDS             25871..26809
                     /gene="galU"
                     /locus_tag="IVW29_000870"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7838370.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26934..27374
                     /locus_tag="IVW29_000871"
     CDS             26934..27374
                     /locus_tag="IVW29_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7838371.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27377..27979
                     /locus_tag="IVW29_000872"
     CDS             27377..27979
                     /locus_tag="IVW29_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7838372.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28042..29145)
                     /locus_tag="IVW29_000873"
     CDS             complement(28042..29145)
                     /locus_tag="IVW29_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7838373.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29355..30356
                     /gene="ccpA"
                     /locus_tag="IVW29_000874"
     CDS             29355..30356
                     /gene="ccpA"
                     /locus_tag="IVW29_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7838374.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30711..31106
                     /locus_tag="IVW29_000875"
     CDS             30711..31106
                     /locus_tag="IVW29_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838375.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31106..31357
                     /locus_tag="IVW29_000876"
     CDS             31106..31357
                     /locus_tag="IVW29_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838376.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31400..31795
                     /locus_tag="IVW29_000877"
     CDS             31400..31795
                     /locus_tag="IVW29_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838377.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31792..33048
                     /locus_tag="IVW29_000878"
     CDS             31792..33048
                     /locus_tag="IVW29_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7838378.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33060..33383
                     /locus_tag="IVW29_000879"
     CDS             33060..33383
                     /locus_tag="IVW29_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838379.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33376..33642
                     /locus_tag="IVW29_000880"
     CDS             33376..33642
                     /locus_tag="IVW29_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838380.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11283 a         5793 c         7246 g         9354 t
ORIGIN      
        1 cgttagttcg agagctcttt tttttgcttt gctaccttgt tccttgaaga ttctgctgaa
       61 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
      121 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      181 agtgaatatt ccgaaaaaag gcggaaaagc ggtccaggta aaatcaaaac gcccaacgaa
      241 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      301 attttcatcg attttagggt cttttgttat tttctaggtt gaacatatct aaaaaattgt
      361 ttctgacaat aggagtaggg agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      421 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      481 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      541 aaatgaaatc gaacagggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      601 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      661 tgaaggaatc ttagaaagaa agatcggttc gggtacgtat gttgcgcgag aaaaagtaca
      721 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcacaaa atcgagtacc
      781 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      841 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      901 ccctatttgt tttgaagttg ccagtgttcc acaaaagctg atcgatggct acagcaaagc
      961 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
     1021 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1081 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1141 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1201 aaaaacaggt gatccctgat tccttattag aaataaaaga gaatcagaat cacctgtttt
     1261 tatatatgat cagttatcag ttgctcgtga taagaaacag aaccctcttc aaattggact
     1321 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1381 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1441 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1501 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagaccgc atgagtgtat
     1561 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1621 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtacgcc cgccgttcca
     1681 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1741 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1801 tggtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc
     1861 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1921 ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc
     1981 gcaattgctg tagtatgcga taataaagac aaaggcaggt ggatctgggg atgcaggaac
     2041 gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa
     2101 cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag
     2161 caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg
     2221 gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt
     2281 tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt
     2341 tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcatag
     2401 tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc
     2461 tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat
     2521 cgactcggat attttcgctg tgtccgcttc tgacgcagat aacaaccttg caaataaaga
     2581 cgtggatgtg ttgttattag gcccacaagt tcgctttatg aaagctgatt ttgagaaacg
     2641 tctggaacca aaaggcattc ctttagatgt catcaatatg gctgattatg gtatgatgaa
     2701 tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa
     2761 ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg
     2821 aagaatatga atcatttttg gggtagaagg cttctgacga gagatattga agcaatagac
     2881 tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac
     2941 tggattcttt gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat
     3001 tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta
     3061 tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag
     3121 cttactgaaa aacaaacgga aatctcaaag agaatacttt gccattcaga aaaaagacat
     3181 catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg
     3241 gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat
     3301 gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg
     3361 aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac
     3421 cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac
     3481 ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc
     3541 tacttaagtg ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc
     3601 cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg
     3661 aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt
     3721 catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat
     3781 cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca
     3841 agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgatatactg
     3901 ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta
     3961 ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat
     4021 cgtaaagggc cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct
     4081 attcgaaaag cttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga
     4141 ggtgcagttg ctgcagtaaa gatatcttcc aaaatttaac attcagcgag attattggtg
     4201 tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg
     4261 aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa
     4321 agaaagtaaa gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc
     4381 cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc
     4441 tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca tataaatgga
     4501 cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa
     4561 ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt
     4621 ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg
     4681 aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata
     4741 aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag
     4801 attgtttgca ggtcgcacag ccgaaaatca tccgaaccat ttctttggcg agatgaatta
     4861 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
     4921 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
     4981 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
     5041 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
     5101 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
     5161 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
     5221 ttggttgttg ataaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
     5281 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
     5341 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
     5401 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
     5461 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
     5521 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
     5581 agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga
     5641 agttcttact ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa
     5701 agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg
     5761 ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca
     5821 ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac
     5881 aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac
     5941 ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattgggg ttatatccat
     6001 accacgtaca attgatgggt ggaatcgtgc ttcacgatgg aaatatccct gaaatgcgta
     6061 ctggtgaagg taaaaccttg accgcaacga tgccagtata tctaaatgct ctgactggcg
     6121 aaggggtaca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg
     6181 gcgaacttta taatttctta ggattaacag tggggttgaa tatcaactca aaatcttcgg
     6241 atgaaaaacg cgaggcttat aattgtgata tcacttatag tacgaataat gaattaggat
     6301 ttgactactt gagagacaat atggtcgttt accgccatca aatggtacaa cgtccgctga
     6361 attatgccat tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga acgccgctga
     6421 tcatttcagg gcaagcagaa aaatccacag ctttatatac aagagcagac aacttcgtga
     6481 aacgtttgaa agaagaagaa gattataaaa tcgatgtaca atcaaaaaca atcagtttaa
     6541 ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt ggataactta tacgacattg
     6601 aaaatactgc attgacccat cacttggatc aagcattgag agccaactat atcatgcttt
     6661 tagatatcga ttatgttgtc caagacggaa aagtactgat tgttgaccag ttcactggtc
     6721 ggatcatgga tggacgccgt tattcagatg gacttcacca agcaatcgaa gctaaagaag
     6781 gcgtagagat cgaagacgaa acgaaaacca tggcgacgat cactttccag aactatttcc
     6841 gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa aacagaagaa gaagaattca
     6901 gagaaattta taacatccaa gtataccaga ttccgacaaa tcgtccggtt atccgtgatg
     6961 atcgtccaga tttactttac ccaacattac aaagcaagtt ccacgctgtc gtgcaagata
     7021 tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat
     7081 ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga
     7141 aaaatcactt taaagaagca gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga
     7201 ttgctaccaa catggctggt cggggaacag atatcaagtt aggacttggg gtaagagaac
     7261 ttggtggtct agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc
     7321 ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acaattctac ttgtcattgg
     7381 aagatgattt gatgaaacga ttcggttcag aacgaatcaa agctttctta gatcggatga
     7441 agatcggtga ggaagatgct gttatccaaa gcaagatgct gacgaaacaa gtagaatctg
     7501 cacaaaaacg tgtagaagga aataactatg acacacgtaa aaacgtctta caatacgatg
     7561 atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg acaagaagta atcatggaag
     7621 aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag
     7681 atgcgcatac acaattagag aaagaaaatt ggaatttgga aagtatcatc gattttgccg
     7741 gcagtactct tgtccatgaa gatagtatta ctcttgcaga tatcgaagga aaaacatctc
     7801 aggagatcaa agagtatcta aacgaacgag cacaagaagt atttaatacc aaagcggctc
     7861 aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg
     7921 actctaaatg gacagaccat attgatgcaa tggatcaatt acgtcagtca atcggcttac
     7981 gtgcttatgg acagaacaat ccattagtcg aatatcaaac agaaggctat aagatgtttg
     8041 aagatatggt aggcgccatc gagtatgaag tcactcgaat ctttatgaaa gctgagatcc
     8101 gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc ttctcactca gaagacggag
     8161 atgccaaaga aaatagtaat actagcgcga aaaaacagcc aatccatgtg aacaaggtcg
     8221 gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt catggaaaaa
     8281 atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttta agaattcgtc
     8341 tcaccttctt tctgttattt gctgattctg ttagactaga tataatgaaa aaagtatagt
     8401 gaaagaagga aaaaacatgg aaatatccga aattcgaaat ctcctgacag aaatgcagga
     8461 aaaggtcaca agtttcagga ggtctctttg acttagatca gctggaagaa gacatcgcgg
     8521 aagcagaagc aaaaatggct gaacctggtt tttgggatga cagcgaagcc gcacaaaaag
     8581 tgatcagcga aaataacgct aataaagaaa cctatgacca attccattct cttgccgaag
     8641 aactagaaga aatcgaattg atggtggaga tgctgcaaga agaacctgat gcagcaatgc
     8701 aagaagaagc agaaacacgt atcaatgcat tagatgaaaa aatgaagacg tatgaattat
     8761 ctatgctgtt agacggacct tatgatcgaa acaatgcgat cgtcgaattg catccagggg
     8821 ctggcggaac ggagtcccaa gactggggca gcatgctttt acgtatgtat acacgttggg
     8881 cagagcagca tggctatcaa gtagaaacac tagattatca agcaggtgat gaagcaggga
     8941 tcaagagtgt gaccctgctg attaaaggac ataacgcttt tggttatcta aaatcagaaa
     9001 aaggtgttca tcgccttgta cggatttcgc cttttgactc agcgaaacga cgtcatactt
     9061 ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata gaaatcaatc
     9121 cagatgattt gaaaatcgat acgtaccgag caagtggtgc aggtggtcag cacatcaata
     9181 aaacagaatc tgctgtacgg atcactcact tgcctacagg aacagtagta gctagtcagg
     9241 cacaacgctc acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt
     9301 atcaattaga agtggaaaag aaagaacaag aagcagctgc acttcgagga gaacaaaaag
     9361 aaatcggctg gggttcacag atccgttctt atgttttcca tccctattct atggtaaaag
     9421 accatcggac aaacgtagag acaggaaatg tccaagcagt tatggacggt gaccttgatt
     9481 tatttattga cggctattta aaaatgcatc tacaataaga actcattgtt aaattgaaat
     9541 aaaattgtaa gattttgcaa gcatgttgaa acattgccat gctataatag ctaaagactg
     9601 aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga agtactcaaa
     9661 tggtacgacc gctatccgca atctctcgat tgtcatcgaa caaggagagt ttgtctatgt
     9721 ggttggtccg tctggagccg gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa
     9781 agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagcaatca aaaaccgcga
     9841 agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat tattaccaag
     9901 aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa aacctcgaga
     9961 tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca aagttcgagt
    10021 ctttcctagc gaattatccg gtggggaaca gcaacgtgtc tctattgctc gggcaatcgt
    10081 gaatacgcct aaagtactta tcgcggatga accgacagga aatctggatc cagaaaactc
    10141 atgggaaatc atgaaattat tagatcggat caatgcccaa ggaactactg tcgtgatggc
    10201 tacacataac agtacgatcg taaatacgat tcggcaccga gtaattgcta tcgaaaacgg
    10261 ccgcattatt cgagatcaag cggaagggga atatggatac gatgattaga acctttttcc
    10321 gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca gttgcttcag
    10381 tcagtgcggt aacgatcacg ttgactttag tcggtgtatt catggcagtt atcatgaatg
    10441 caactaaatt agctcaggat atagaaggaa acgttgatgt atctgtattc gtagatatcg
    10501 gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt gaatgactta gatcatgtta
    10561 aaaaagttga attttcaagc aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg
    10621 attcctggaa attgtttgaa ggtgacaata acccgttata tgatgtgtat gtagtaagtg
    10681 ctactgatcc ttcttataca aaacaagtag cagaagaagc agcagatttg aagaatgttt
    10741 ctcgttctga ttacggtgga gcttcctcag atcggatttt ccaaatcgct ggtgcagttc
    10801 ggacatgggg attagttgca gcggcattgc tgctgttcgt agcgatgttt ctcatttcga
    10861 acacgattcg gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg
    10921 gtgcaaaaaa tggatatatt cgctggccgt ttttccttga aggtggctgg atcggtctga
    10981 tcggtgcgat tcttccgatt ttgatcatca catttggtta tgcatggttt ttccaattga
    11041 tcaatccatc attattacgt tcccattatt cattgattcg tccacaaaat atcgtttgga
    11101 aaatcaatct tttaatggtc ggcattggtg tgatcattgg ttcacttggc tcgatcattt
    11161 ctatgagacg ttttttgaaa ttctaatacc acaatataaa taaaatgaga aagttggaca
    11221 gtttcgttcc agctttcttt ttttattggc taggagaata gaaggaagcg gcttttttcc
    11281 ctttacttta aaaaacaact tgggtattct taagttggta gaagaagagg tgaagccgtg
    11341 aaaattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt gctttccttt
    11401 atcatcgttt ttcgagaaag aaaggaaact gctcaaacat gggcatggct gctcgttttg
    11461 atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg gatttccaaa
    11521 gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga aattgaagaa
    11581 gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca agtagatgtg
    11641 aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac taaaaatgaa
    11701 atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga tatcaagcag
    11761 gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt aggtgaagaa
    11821 gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt ccttcttgat
    11881 gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa agcaggcgga
    11941 gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat caattatcgc
    12001 aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg gttcaatgtt
    12061 ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa tcatttgcgt
    12121 atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg gaattctcag
    12181 catacaaaag aagtaggcta ttctcccaaa tttttcccgt ccattcacac tactggtgat
    12241 atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat caaaatgact
    12301 tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc ttattacata
    12361 ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt aaaagttcat
    12421 atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta ttctttcgct
    12481 gcagagctga tcgagtatgg agcaaggatc gaaacatatg agaatggatt tattcatgca
    12541 aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat cgatgttcgt
    12601 tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat ggcttcacgt
    12661 gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa agaaatgtat
    12721 gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat ttcgccgctc
    12781 ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt gtccttttct
    12841 aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat gagcgaaatt
    12901 aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata tatgctatta
    12961 aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata gtttttttta
    13021 caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc aagtaatgaa
    13081 aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa acttaaatta
    13141 ttcttaccaa taataggatt agccattctt ttatcaggct gttcaaagtg gattgatcga
    13201 ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga aacagttgct
    13261 gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga
    13321 actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga tttgtttgcg
    13381 gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc agttgttggg
    13441 atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga aaacctgaaa
    13501 aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga tcagccagta
    13561 gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa atgggtattg
    13621 gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt gcgccagatc
    13681 gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc cgatgatgta
    13741 gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg
    13801 acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact gacaaaagaa
    13861 tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga actaggttat
    13921 atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt taaagagtaa
    13981 tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa ccaaattgat
    14041 gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc atctgtggaa
    14101 gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa
    14161 ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat tttttatttt
    14221 gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt tgattttctg
    14281 tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca
    14341 ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt tgccacgcca
    14401 tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg aacgaagatc
    14461 ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg ttttatcggt
    14521 ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggacagggtt tggtatctta
    14581 gctggtacat ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat gactgttgat
    14641 gcattaaagg cagtaccacg gcactatcgg gaagcttcat tagcgcttgg tgccacaaga
    14701 tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt aacagcagtc
    14761 gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt catcggtaat
    14821 gcagcattga tgccaacagg acttactact cctgcttcta cattgacgtc catcttaaca
    14881 atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg gtcattagcg
    14941 ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat tgggaagaaa
    15001 ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt gttttatata
    15061 tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc ttagttcggg
    15121 gaataccgca catttcatgg tccttcttga ccgaaccttc caaagcctac caagaaggtg
    15181 gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg atgatcatca
    15241 gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga aaaaactggc
    15301 taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg
    15361 gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg attatttctg
    15421 gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt gaggagtctt
    15481 tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg tcacgctggg
    15541 aaacagtgat gaaagtggtg gttccagaag cgacaccagg gattttgact ggtgtgatcc
    15601 taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct ggacaaagtg
    15661 cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg atcagcattt
    15721 tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa ggcacgatgc
    15781 ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gatcattgct gttttactgt
    15841 tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact tcagcctaag
    15901 gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca ccatgccaaa
    15961 agagaaagaa gtagcactat atacggatga tatacatgtc tggtatggcg acaatgaagc
    16021 aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga ttggtccttc
    16081 cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg ggatcgctaa
    16141 tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc ctcaagtgga
    16201 tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa
    16261 gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa
    16321 attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga
    16381 cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt tatgcattgc
    16441 tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa gtgctttgga
    16501 ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag actatacgat
    16561 catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata ctgccttttt
    16621 ctatctagga aaagtaatgg aatatgacaa aactaacaag atatttacca gaccaaaaat
    16681 tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc tcaacatgac
    16741 aaaagaaatt atttcatcta aagatcttca tctgtattat ggaaagaaag aagcactaaa
    16801 agggatcgat ctaagtttca atcaagggga aatcactgcg atgatcggtc cttctggttg
    16861 cggaaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc cgaatgtaac
    16921 gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa cagatgttgt
    16981 tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc ctttttctat
    17041 ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa aagtccttga
    17101 tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt
    17161 gcataaaagt gctttatctt tatccggcgg gcagcagcag cgtgtatgta tcgcacgtgt
    17221 tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt
    17281 ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata cgatgatcat
    17341 cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat tctttttaga
    17401 tggtcacttg atcgaataca acgatacgaa gaagatattc atgaatccag aaaaacaaga
    17461 gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc tacgtacaca
    17521 atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga tgatggtgag
    17581 cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct tagcccaaga
    17641 agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga agaaaagttt
    17701 tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca ttacggtcat
    17761 gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac
    17821 gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc
    17881 cgattatgtg aaaaaaatgg tggacaacgt cctagttgct tatgtcaaaa cagacgaaaa
    17941 agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca atcgaatcta
    18001 taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta
    18061 cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca tctgtgaatg
    18121 gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca atgagatttt
    18181 ctaaactgaa gctgtgatta tgccgtttgt ttatgaaaag aggttgtgaa aaagctgttc
    18241 tacatcaagt aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg
    18301 gacttattac cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca
    18361 aaacatgtca caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat
    18421 tagcatcatc actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc
    18481 cgctttatat gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca
    18541 attgattaca ggaaaactga tgaactcgga tcaaaaagga gaatcgattt atttatcggt
    18601 tccttatcaa aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt
    18661 ccaaatgcat ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt
    18721 cttcacaagt atcttcattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc
    18781 tttaggaagt ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa
    18841 aacagaaggg atttctctat taaataaatc tatcctgttc gtcgttatag ggatgattat
    18901 tagcatattg gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt
    18961 tacgagtgtt tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca
    19021 aggactgcgc tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc
    19081 tagcgtactg attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac
    19141 attagaagaa ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg
    19201 aaggcaagtg ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat
    19261 cagtgggtca gttccatcgc ttcttttata tttaggaaat caatggtctt tcaaagaaac
    19321 gatagaacag catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt
    19381 attatcgatc cccatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat
    19441 gtacttatta tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa
    19501 ggtatgtata ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc
    19561 ggttatcatc agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct
    19621 gtggttatct tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt
    19681 atactgatct ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc
    19741 caaggtttta catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt
    19801 gattttagat tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat
    19861 gcgagtgtag caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct
    19921 ttttaccaat tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta
    19981 atgacacttc tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat
    20041 ttagacattc cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg
    20101 gctattctta ccacattgtc cgcactaatt gttcttcctt taacctgcgt gactacagca
    20161 tatctcttta caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc
    20221 tatgacattt ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt
    20281 ttgcttaagg aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa
    20341 ggaatccttt cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac
    20401 gaaaagcaaa tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagataag
    20461 gcaagtcaat tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat
    20521 ccaaaggaaa gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca
    20581 actgaagcga ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat
    20641 aaggcagcac gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta
    20701 ggcgagttga cagaagaaga attagcacag cgtcaggcgt tagaggcaga aatcaaagag
    20761 ttagaagcta gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa
    20821 gatatccgta agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg
    20881 ccagatgatt ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca
    20941 gaagctggat cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca
    21001 gtgaatgaca acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg
    21061 aaatttgagc atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca
    21121 gcaaacggaa acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag
    21181 atcaaacttt acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag
    21241 attgaggtta atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac
    21301 cttgttttct acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc
    21361 aacatcatga ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac
    21421 atcatcgtgg accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc
    21481 gatattcgaa acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca
    21541 tttggtgcga cagcagaaaa tctttctgtg tctttagtaa atggtgatgt acgtcttacg
    21601 attaaagaag ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc
    21661 gctttaccag atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaat
    21721 agccgtctgt ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg
    21781 caattccgtc gtgtttcaga tgatgagatc gcgcaagtgc aactttcaac gacaacagga
    21841 agtatctatt taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga
    21901 gcaataagaa gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc
    21961 cgatagtcta tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg
    22021 agtcacaagg aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga
    22081 ccgagaattt tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat
    22141 ttaaaatagt agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa
    22201 atgttgtttt gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg
    22261 ttatccgtgt gctttatgta ttcctgagct tagttgcttt tggaagtcca attattttat
    22321 acattttatt agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata
    22381 acaatgcata ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac
    22441 gcaaagaagc ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca
    22501 acgtatttta gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt
    22561 ccatgtgagc agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat
    22621 gtttgtcaaa ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt
    22681 gtttacgttt gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga
    22741 acattttagt ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt
    22801 caatgtaatt gtcagcgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg
    22861 gcgcagtttt tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa
    22921 aacgtttttt ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact
    22981 ctaatagaaa tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa
    23041 aattatcttt ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg
    23101 gggacatctc acgcccagga ctagagttga ctggttattt taattattat tcccataatc
    23161 gtttacagct atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc
    23221 gtttgatcat catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag
    23281 gattaccagc tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc
    23341 gttctcccat ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc
    23401 ttgcccctcg aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga
    23461 ttcaaggtga cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc
    23521 atcgattgat cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg
    23581 gagaaccgcc taaaatcctg gaacatctga ttgaaatccg aggaatcggg atcattgatg
    23641 taatgaatct ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt
    23701 atctggaagc atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg
    23761 ttgaaatcgc ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg
    23821 ttgcaatcat cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg
    23881 caacgaaaac atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact
    23941 agcaaggagt gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat
    24001 tcctatctac tggtatgcat tgatcatcgt atcaggtatc attatcgcta tgtggcttag
    24061 cagcagagag gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg
    24121 gggattgcct atctccatta ttggcgctcg cttatattat gttttatttg atcttcccca
    24181 gtatatagct gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg
    24241 cggactgatt gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt
    24301 gtggactttt ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg
    24361 gggaaatttc atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag
    24421 tctgcattta ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc
    24481 aacatttctt tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg
    24541 aaagaaacca caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag
    24601 tttcgggcgt ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat
    24661 ccgtatttct caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg
    24721 gcgcagaaaa aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat
    24781 tatctgattt ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata
    24841 gagtagcggt tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa
    24901 acggacatga cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc
    24961 aacacacgaa tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca
    25021 ccaatatgga agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag
    25081 caatacgttc tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc
    25141 atgcaagtaa aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg
    25201 aaatcccagc agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag
    25261 aagtagcaaa acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa
    25321 aatatgtaca agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga
    25381 tcggtgtaga gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc
    25441 acggcttagg gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga
    25501 tcagccgttt aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggaa
    25561 taggcgatct agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact
    25621 tgttaggaca agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag
    25681 gtgtgaatac tacccaagca gcgatggaac tagcccaaat gatggaaatc gaaatgccca
    25741 tcactcaaac aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag
    25801 aaatcatgtt acgtgatgga aaaaaagaaa atgaattctc attaacaatt ttttgaagga
    25861 gcgaatgtat atgaaagtta aaaaagcagt tattccagca gcaggattag gaacaagatt
    25921 tttaccagca acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat
    25981 ccagttcatc gttgaagaag cgttgaaatc aggtatcgaa gacatcttga tcgttacagg
    26041 aaaagctaaa cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct
    26101 aagtgaaaaa ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt
    26161 gcatttcatc cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc
    26221 ctttattgga aatgaaccat ttgtcgttat gctaggcgat gatataatgg aagacgaagt
    26281 accattgaca cagcaattga tcgatgacta tgagcgtaca cacgcttcta cgattgccgt
    26341 tatgcaagtt cctcacgatg aaacttcaaa atacggaatc atcaacccag gggaagtact
    26401 ggaagatggg ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc
    26461 aagtgattta gctattattg ggcgttactt attgactcca gaaatctttg atgtattatc
    26521 agaacaacaa ccaggtgctg gaaatgagat ccaactaact gacgcaatcg atacgttgaa
    26581 taaaacacaa cgcgtatttg cccgtaaatt cacaggtaaa cgttatgacg ttggtgacaa
    26641 atttggtttt atgaaaacaa gtattgaata tggtctgaca catccagaag taggggcacc
    26701 tttgcgtgaa tacatcatgt ctttaggtaa aacattagaa gcagaaggaa gcaagatcaa
    26761 tcataatcca aaaggaataa cagaaatcaa agaagaaaag aaaaaataat ctttttttct
    26821 aacgagcttc agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa
    26881 aaacacgaaa atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag
    26941 gaggagaaat tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gttttcatcg
    27001 ttcgcgtatt gttgcaagtt tccaaaactt tagaaaaagt agaccagact gtcgcagaag
    27061 caaatacgac aattgagatc gtcacacaag acgtagatct tttatctaga caagtagaag
    27121 gcttgctagt caaaagtaat gagctgctct tggatatcaa tggaaaagtc gcaacgatcg
    27181 atccattgtt cacagcagtt gctgatttaa gtgaaagtgt atcggaactt aatcattcta
    27241 gtaaaaacat cgtgacgaaa gtcggcggaa tcggaaaaac gacagctaaa gcgactgttg
    27301 ccggaaaagt cggtggaaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga
    27361 caggaggaaa ataagaatgg ctaaaaaagg tggattttta ttaggtgcag tagttggagg
    27421 tacagccgca gcaatcgccg cattattgtt agcacccaaa tctggtaaag aacttcggga
    27481 tgaattggct gctcaagcag atgatttgat ggacatggct tcagattaca cagatatggc
    27541 aaaagaaaaa tcatctgagt taacaggtat tgcaaaagaa aaagcgcaag gtctttctca
    27601 acaagcaggg gatctagcag gcaatgttaa gcagaaggta agtgaatcag cagaggaagc
    27661 aggaagtgaa tcagatgata tcttgttaaa cctcaaagaa caatcagctg atttatctaa
    27721 tcgtttcaaa aaaacagcag aagatctgaa aaagccagca gacgaattag gtagtattgc
    27781 taaagatact tcagaagata tcatgatcga tgtaaaagat gctgcagata aagcaggaga
    27841 gacggtcaat gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgtt
    27901 gaaaatggca aaagaagatg caaaagaagc taaaaaagaa atgaaagata caatgacaga
    27961 ttcgactacc aatcactaag attactcatt tctagaataa aaaaggtgag agcggaccag
    28021 ctctcacctt ttttattttc attactctaa aacaagtaat tcttttggtg tagtcgtgaa
    28081 tggcacacaa ccatcacttg tcacatacac acaatcttcg atacgtaccc ctactttttc
    28141 aggaatatag attcctggtt caagagagaa gcacatccct tcttcaatca ctaagtcgtt
    28201 tccttctaca agagaaggga attcgtggac ggttgttccg attccgtgac ctaaacggtg
    28261 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    28321 acctgctgtc acacctgggc gaactgcctc ttgtgcggca agctgtgctt caagagtgat
    28381 gttatagatt ttttcttgga actcagttgg tttttgataa gcaactgtac gagtagcatc
    28441 actgcaatag ccattccaaa taacgccaag atcgaataac actagttcat ttggtgaaac
    28501 cttcgtgctt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    28561 gtcgaaagac atttgtgaaa caccttgttt ttttaattgg tattcgattt ctgcgataat
    28621 tgcttgttca gttacgcctt cttttactgt tttgaagcca atttcaaagg caacatcggc
    28681 ccatttacca gcttcgatca gtttgtcttt ttcttctacc gttttgatca gttgcagacg
    28741 ctggataata gaagttagat cggtgtaaga atctgtacct gtcaagatat cagagatagc
    28801 atcgaagcga tcgacagtca gagcattctt ttcaatacct acattaccag gttgcccata
    28861 gcgtgcgcga agctcttttc cgatgatctc ccaaggattt tcgctgtcta agtagccacg
    28921 tacatcgtac gcccagctgc tgttttgagc atcttcaact tccagagcag gagtgaaaag
    28981 gaaaggatct tgttccagag ggataaaaag agctagtaca cgctcgtgag gatcactttc
    29041 atatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt ctacttggtg
    29101 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    29161 ctaaattaat tttgtattca ataattgtac cactgattga ttaaaagacc aacgttctta
    29221 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attgttgaga
    29281 ttatttgata tcctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    29341 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    29401 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    29461 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaat gcagtcgcac
    29521 gtggcttggc aagtaagaaa acaacaacag ttggggtaat cattcctgat gtcagcaata
    29581 tgttctttgc ctctttagca cgtggtatcg acgatgtggc aacaatgtac aaatacaata
    29641 tcatcttagc aaactcagat ggcaacgatc aaaaagaagt caatgtattg aataatttat
    29701 tggctaagca agtggacggc gtgatcttta tgggtcacca tatcacagac gaaatccgtg
    29761 gagaattttc acgttcaaaa acgcctgttg tactagctgg ttccattgat ccagatgaac
    29821 aagtaggtag cgtaaacatt gattatgtgt cagcaacaaa agatgcagta aataaattag
    29881 caaaaagcgg caataaaaaa atcgcttttg tcagcggcgc gctgattgat cctatcaatg
    29941 gtcaaaaccg tctaaaaggc tataaagaag cattaagcga aaacaatcta ccatatagcg
    30001 aaggattgat ttttgaggca caatacaact ttaaagatgg tctgtctcta gttgaccgga
    30061 tccataatag cggtgcaaca gctgtctatg tttcagacga tgaattagcc attggtatct
    30121 tggatggttt attagaccgc ggcgtaaaag taccagaaga attcgaaatc atcacaagca
    30181 acaattcttt actgacagaa gtagctcgtc ctcgtttgac aagtatcaca caaccattat
    30241 acgatttagg agcggtatcc atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    30301 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatcaact aaataataaa
    30361 agatcaatca tttaaaggtt gttccaaaag tggtcaactt ctggaacaac gtttatacga
    30421 gaaagggggt tatgacaaaa ttttgtcata accccttttt ttttgtgact ttaaaaaaag
    30481 agaaaatggc ggaaacaaac cttacgtcca tcgtatacga cacaatctgt gcgtagatgc
    30541 tacattttgt tcactactat caaaattact ttaaaatgaa aacgtgataa aaaagaaagt
    30601 agaaaagtag ataagtaact ctaaaaaatc caaaaaaatc caggctcgat tgcgtactta
    30661 tcttaaagga gaaacaatcc atcatatata acccgaaagg agacttctat ttgcctaaga
    30721 aaagacgaaa gaccatagtc atcattttga ccattcttac tataggaatc ggagggaaat
    30781 tttacatgga caaaagagaa atgcaaagac agaaagattt actcgcagta gaaaaacagt
    30841 ccgtcaaagt tctgaaaaat acctttgcgg atatcaagga agtgaagatt gaaaaatctg
    30901 ctcgaaatga aatgacgggt tcataccgga tagttattct aatgactaat aaacaagatc
    30961 aatcgattta tttttcttat agtttttgga aagaaagaaa tgagatagga agttacggaa
    31021 tagttgatga aaaaaagcaa aaagaaggaa atacgctcaa taaagttaaa gtaacttact
    31081 ctaatggaaa tgaggaaagt atttaatgat tactgacaaa caatatagtg aattaagtga
    31141 tgctgtttac tggctagatc ctaaacatag agactatgta cctgaaatgc aagaaaattt
    31201 aagttttaaa attaacggca ctatgtataa aatcctaaaa atcaaaaact ccttcgacgg
    31261 catgcaggcg atggctgtag cgccaatcgt ccatagcaaa ttggagaaaa atttcaaaaa
    31321 taaaaaaatt cctgcaaact ttcgcgtact taaataatga aggtgtcaat acttacataa
    31381 aaagaaagga ggcacgttct tgtcaaggaa aataagaaaa ctcagcttag ccattttatt
    31441 aatcgtcata ttagggatcg gagggaaagt ctatatggac aaaagagaag cacaaaggca
    31501 gaaagattta ctcgcagtag aaaaacagtc agtcaaagtt ctgaaaaata cctttgcgga
    31561 tatcagggaa gtgaaggtag aagaatttaa aaaaaaccct gttaccggaa gttatggtgc
    31621 acttgtgaca atgacaaata atgaagggaa atctgtttat tttgattata gtttttggaa
    31681 ggagagaaat gaacttggtg gttttggcat tgagaacaga gcggtacaaa agacaggagt
    31741 cactataaaa agagtaaaag taattttttc taacggacag gaggagttag tatgacaata
    31801 aacgataagc attacaatga tattagtgag aaggtttatt ggctagatcc caaatatccc
    31861 cgctacaatg agggatacaa aaagaactct gttaaagaat ttgctggtat ggaatttcaa
    31921 atcctacaaa tcaaagactc attggacggc atgcaggcga tgtctgtagc gccaatcaag
    31981 aacggtaaag tggatctttc tcaagtagtg attgcttttg caggaacgaa cttaagtgat
    32041 tggaaagatt tggaaacgga tgcaagaagt atcatgaatc tctacgggaa tagaccagga
    32101 gcaagttttt ctgaaaaagc aacaacaaac aagattttat ccgttagtgg attgaaaaca
    32161 gcggggcaaa tcaattcagc gattgcattt gctaatgaag taaaaaagga atatggagaa
    32221 gctgctattt ctactaccgg ccattcatta ggtggattct tagcattata tgtagcagca
    32281 gagaaacaat ggaaaaatgt cggcttcaac gggccggacc cgtatgatct cttatcacca
    32341 gaagcaaaaa aatgggtgga agaaaatccc ggtatgctca ccaattaccg aaatcacgcc
    32401 gattttatcg gtaattttgg tggtaatggc acaggtgctg aagttcggat cagtatggaa
    32461 acagcattct tgaatctgct tgcttcccac agtttaacct cttggaagtt tgatgaaaat
    32521 ggaaagctga ttattcctga aaatgaaaac aatgttaaag ctcgaaaaca acagaaagaa
    32581 aaccaagcga cccagttgtt ttcctatcag ttgcaagaac tgaacaacat acgtaaaaaa
    32641 ctacgattaa gtggcggccg gctgacagtt aatgaacaaa tttacttaga tgacacacaa
    32701 gctaggttag tagtggaaaa gttatcggca aaaatgaaaa gcgctatgga agaagttatc
    32761 cgcatcaatc aaagaggaat ttttgagcta gagaaaaaat ggcaagaagg cttgcggcaa
    32821 gcacgtgttg tagccccttc gttgactgaa caggaaatca tagaagcatt agctagtgtg
    32881 ggcgccacaa aagaaaactt agtcgattct gcgaaagaag tctatctgct acgaatcaat
    32941 aaggcaagac gtatggggga ggcctttgat acattggcga aggagatcca agacaaaatc
    33001 aatgaaatcg tggcacgaga tcgagaatta gcacagcaat tcaattaaag gagaagtaga
    33061 tgaaactaga agaattggaa agagattatc gaagtgaacg aagacaatta gaagctcaag
    33121 aagatgaatt gatccacttt aagaatcaag gattacgatt gatggagcaa gaggagcagc
    33181 gactgcatca tgtattaaag aacttagctt tagataatga gcctctgttt caagcaagac
    33241 gaagttattc gaattatcag gagcattatc aacaagccct acaagtagaa cgacgaaaac
    33301 tctatcagca gcaagatgaa ctagaacgtt cctatcgtca aaaaatcaga caaataacta
    33361 tggagaaatc atcttatgaa tgaattttta atgcaattac taaaagaatt agagaaaaat
    33421 ctacatcgat caaatgtacg ttcagttggt atggggatat ctcagcaaat ttgggcagaa
    33481 aaacgattga cagatcaaaa ctaccaaatg actgcagaag aagctgctca agaattttct
    33541 aaccgacatg tacacttgaa aggatcagta aaagggcaat ttgcagaaaa actaactgaa
    33601 ttattccaaa gacaagaaaa gaaattaaaa cagtttcgtt agtaaagtta tatagaaaaa
    33661 ttttgtgaaa atggtt
//
LOCUS       DACCVU010000022        32309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCVU010000022 DACCVU010000000
VERSION     DACCVU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.022"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            15..1913
                     /locus_tag="IVW29_000881"
     CDS             15..1913
                     /locus_tag="IVW29_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAP7838381.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2243..2962)
                     /locus_tag="IVW29_000882"
     CDS             complement(2243..2962)
                     /locus_tag="IVW29_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7838382.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3299..3631
                     /locus_tag="IVW29_000883"
     CDS             3299..3631
                     /locus_tag="IVW29_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838383.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3618..5621
                     /locus_tag="IVW29_000884"
     CDS             3618..5621
                     /locus_tag="IVW29_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7838384.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5640..6110
                     /locus_tag="IVW29_000885"
     CDS             5640..6110
                     /locus_tag="IVW29_000885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7838385.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6131..6715
                     /locus_tag="IVW29_000886"
     CDS             6131..6715
                     /locus_tag="IVW29_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7838386.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6730..7716
                     /locus_tag="IVW29_000887"
     CDS             6730..7716
                     /locus_tag="IVW29_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7838387.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7706..8017
                     /locus_tag="IVW29_000888"
     CDS             7706..8017
                     /locus_tag="IVW29_000888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7838388.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8039..9820
                     /locus_tag="IVW29_000889"
     CDS             8039..9820
                     /locus_tag="IVW29_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7838389.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9813..11189
                     /locus_tag="IVW29_000890"
     CDS             9813..11189
                     /locus_tag="IVW29_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7838390.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11196..11831
                     /locus_tag="IVW29_000891"
     CDS             11196..11831
                     /locus_tag="IVW29_000891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7838391.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11861..13216
                     /locus_tag="IVW29_000892"
     CDS             11861..13216
                     /locus_tag="IVW29_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7838392.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13408..14802)
                     /locus_tag="IVW29_000893"
     CDS             complement(13408..14802)
                     /locus_tag="IVW29_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7838393.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14894..15865
                     /locus_tag="IVW29_000894"
     CDS             14894..15865
                     /locus_tag="IVW29_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7838394.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15862..16644
                     /locus_tag="IVW29_000895"
     CDS             15862..16644
                     /locus_tag="IVW29_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7838395.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16813..17184)
                     /locus_tag="IVW29_000896"
     CDS             complement(16813..17184)
                     /locus_tag="IVW29_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7838396.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17318..17761
                     /locus_tag="IVW29_000897"
     CDS             17318..17761
                     /locus_tag="IVW29_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7838397.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17804..18613)
                     /gene="yidA"
                     /locus_tag="IVW29_000898"
     CDS             complement(17804..18613)
                     /gene="yidA"
                     /locus_tag="IVW29_000898"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7838398.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18632..19999)
                     /locus_tag="IVW29_000899"
     CDS             complement(18632..19999)
                     /locus_tag="IVW29_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7838399.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19983..20813)
                     /locus_tag="IVW29_000900"
     CDS             complement(19983..20813)
                     /locus_tag="IVW29_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7838400.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20976..21413
                     /locus_tag="IVW29_000901"
     CDS             20976..21413
                     /locus_tag="IVW29_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7838401.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21489..22109
                     /locus_tag="IVW29_000902"
     CDS             21489..22109
                     /locus_tag="IVW29_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7838402.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22298..22804
                     /locus_tag="IVW29_000903"
     CDS             22298..22804
                     /locus_tag="IVW29_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7838403.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22821..23720
                     /locus_tag="IVW29_000904"
     CDS             22821..23720
                     /locus_tag="IVW29_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7838404.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23807..24691
                     /locus_tag="IVW29_000905"
     CDS             23807..24691
                     /locus_tag="IVW29_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7838405.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24883..24954)
                     /locus_tag="IVW29_000906"
     tRNA            complement(24883..24954)
                     /locus_tag="IVW29_000906"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24919..24921),aa:Arg,seq:ccg)
     gene            25014..26348
                     /gene="rpoN"
                     /locus_tag="IVW29_000907"
     CDS             25014..26348
                     /gene="rpoN"
                     /locus_tag="IVW29_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7838406.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26551..27588
                     /locus_tag="IVW29_000908"
     CDS             26551..27588
                     /locus_tag="IVW29_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7838407.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            27629..28630
                     /gene="gap"
                     /locus_tag="IVW29_000909"
     CDS             27629..28630
                     /gene="gap"
                     /locus_tag="IVW29_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7838408.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28740..29930
                     /locus_tag="IVW29_000910"
     CDS             28740..29930
                     /locus_tag="IVW29_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7838409.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30034..30789
                     /locus_tag="IVW29_000911"
     CDS             30034..30789
                     /locus_tag="IVW29_000911"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7838410.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30905..32203
                     /gene="eno"
                     /locus_tag="IVW29_000912"
     CDS             30905..32203
                     /gene="eno"
                     /locus_tag="IVW29_000912"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7838411.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10657 a         5703 c         6886 g         9063 t
ORIGIN      
        1 ttggaggcgt tttaatggac cgctatttag atttactttc agagaagttc cctacaacgg
       61 aagatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
      121 agttattttt gagtgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
      181 gttcaggaaa tcttaaagaa aagatcaaag accttttttc tgaacaaatg acggaaacgg
      241 atatcagcca attcactatt tttacggctt atccagaata tgcattaacg actgattggt
      301 acaaagaaca agataaatcg cagctgatcc atcatttgat cgatctttta cggtttacga
      361 cagtcaaata tacccggtca aaagttcgaa aaggattgcc gaaagaatat agctatatca
      421 ttgaggaact cttgtatatc gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa
      481 tcattgaaca acttgttttg atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga
      541 cgatccaaaa atctgtcatc gatcatttgc atatcgttgg agatattttt gaccgaggaa
      601 ctcaagcagc caaagtgatg gatcgaatca tggatttttc ttctgtagat atccaatggg
      661 gaaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttactgacac
      721 ttttacggat tgcagcacgc tataattatt tatttgaatt agagaaagaa tatgggttga
      781 atttacgtcc attgttttct tttgctcatc aaacttatca gaagaatcca gtatttgcgc
      841 ctaaaaacag taaaaatcta tccgaaagag agcaagaaga attagaaaaa atccatcagg
      901 cactttctat ccttcaattc aaatcagaac atcagttatt ggatcgtcga cctgaattta
      961 aaatggacga tcgtaaattg ctggaaaaaa tcgactatga agcatcaacg attgatttag
     1021 aaggtcagct ctatgagttg aaaggaactt gttttcaaac gatccagcca gatgatccaa
     1081 aaaaattctt ggaagaggaa caaaaagtga ttgatagttt gatgtattca ttccagcact
     1141 ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa agggggaatg tatctcacga
     1201 acaatcagca tgtgctgttt catggctgta tacctgtaga cgagaaggga cagctcttag
     1261 catttgaatt agaccaatct tatcgcggaa aagaactgct gggctttttt gaaaaacaga
     1321 taactgaaag cgcaaaagat ctgactgcaa aagaagattt agcaaccgat ttgatttggt
     1381 atgcgtggag tgggccattt tcgcctttat tcggcaagag taaaatggcg acttttgaac
     1441 gttattttct cacagagcct catacgcacg tcgagaaaag caatccttat tatcacttgc
     1501 gagatgaaga gtggttcagt gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt
     1561 cagcaatcat taatgggcac acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag
     1621 caaatggaaa agtatttgtc attgacggag gtttatccaa agcttatcaa aaaacgacag
     1681 gaattgccgg ttattcacta ttatgcaatt cttatggttt ccaaatcgtg acacattatc
     1741 cgtttttgcc tattgaacaa caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag
     1801 taatcgaaca gcaactacca cgcaaactca atcgtgatac aacaaaaggt atggaactgc
     1861 agcagcagat tacccagctc aaaaggctgt tggactatcg aaaggacgat tgagaagaat
     1921 tccagtgaac attgttccat agactggaga ctgagacaaa agtcgtgtcg cagtctcttt
     1981 ttccggataa acggtgttct tctgccaact cctcggtcac aagtcacact attcaacaaa
     2041 catgaaaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
     2101 acctcttggt ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca
     2161 aaaatgtgaa aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt
     2221 cacaaccccc tcagaaagtc tattaaaatt cattcacaaa gaaatcgaat aagcgctgtt
     2281 cagatggatg tacacggtgg gtaagttccg gatgccattg aacaccgagg attttatgca
     2341 tatcatcttt gctttgaacc gcttcaacga gtccatcgtt agaaaaagcg atggctttta
     2401 ataaaggtga aagatctttg actgcctgat gatggtaaga attcacaaaa tatggatcgg
     2461 ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac ttcgtgagtc gtaaattgcg
     2521 gggcagtggg ctgttgttca tgttttactg accactttgg atataatgaa agatcttgat
     2581 aaagtgtacc ttccaaaacg acattcaata gttggagtcc ccggcagact gcaaaaattg
     2641 gttttctttg tttcaatgct tccaagatca gtgcctgttc aaattggtct cgttgaatat
     2701 ttgtttccgt tagctttgga tgaggatctt ccatgtaaag ttgtggactg acatcttgac
     2761 cacctgcaag aataagtttg tcgatttgtc caatcagttg aggagctgaa tctggagaca
     2821 tgactggcag aataagaggc aatccttgag catctaaaac tgcatccacg aatccttgcg
     2881 gtgtatagct cacttgattt ccttggaaag tatctgttgc tcgaatcagt tgatttccgg
     2941 caatgccgat gattggtttc atttgtattc cccctaaagt agacttattg taacaaggat
     3001 ttaaaaaaaa ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac
     3061 tatctgtaaa gagaaatttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt
     3121 tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt
     3181 cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag
     3241 aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt
     3301 gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgaaaagcg
     3361 tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca gaaaaagagc aggaattgga
     3421 aacgatccaa acaaactatc gacaacaact aaaacagttg gtggaagaaa aagaacaatt
     3481 tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca
     3541 agcaactttg aaagatcaat atcaaaagca tcagagtcaa gcgatcggtg cgatcattga
     3601 aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata
     3661 aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaacgtt gaaatccggg
     3721 atctgtatca aaatagaaca aacaaccagt gggtggagaa gtattttcca gataccacga
     3781 ttattgataa agaagcaaaa ttaagcgctt taggtaatcg gttgaaagac atccaagaag
     3841 ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc
     3901 aagaactttc cttacaagat ttggaagcca attattccga agaaaatttc ataaataaat
     3961 tgaaagaagt ccttgattta aaagaaaagt gggaacaact aactgaagaa cgtgatcgtt
     4021 tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt
     4081 atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt
     4141 tcaaggaaga actgagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa
     4201 aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgttata gaagaaattg
     4261 ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaac
     4321 tgcaagagac caagcagcaa ttatccattg ttcacgagca gcaaaagaag ataacgacag
     4381 cgttaggtca gtgcagtggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg
     4441 ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca tgcgacttat ctgatcgttt
     4501 tgcaaggttg ggtagatgca gaagaaaaac agaatttgct gcatgtgtta aacgaaaccg
     4561 tatcagaaga agatatatac gtcagtttcg aaacaccaaa ctcagaagag attcagcagg
     4621 aagtccctac taaattgaaa aaccatccgc ttgtcgaacc ttttgaattg ctgactgaaa
     4681 tgtacagtct gccaaaatat gaagaagtag atccaacacc ttggatgaca ccattttatc
     4741 ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg tttgctgatg ctcattggga
     4801 caatactagc tcaaaaacta cttgttctac cacgcgggat gaaacgtttc gtaaaattct
     4861 ttgagattct ctcgatacca tcgatcatct ggggattgat ctacagttca tttttcggac
     4921 agtcattacc aaaagaactg tttggcatcc gcttgccttt cccaatccta tctacgacgg
     4981 atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag attttggttg
     5041 gattatttgt ggcagcaaag gaaaatatca ggagaaaaga ttacttgagt gctgtcagtg
     5101 atggatttgc ctggcaaggg atcctaattg ggatcgttat tgcgttggtt ggtgccatgt
     5161 tgataaaaaa tacagcattt gtttaccttg gcgctgggct atcgatcatt gctgccttat
     5221 gtatcttgat cgttccgatt atccaaacgc cgtctaaagt caaaggtgct gccaaaggtg
     5281 cgtataatct ttacggactg accggctata tcggtgattt agtcagttat acacgtttga
     5341 tggcattggg tatttcaggc ggaagtattg gagcggcatt caatatgcta gttgctttca
     5401 tgccgccagc tgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga
     5461 atatgttttt gacactgcta agtgcctatg tccatggcgc acgattgcag tatgtcgaat
     5521 tttttgggaa gttttataca ggaggcggac gtgcctttga tccgctgaaa acggcggaaa
     5581 aatacgtcaa catcaatcac aagaaaaaaa acaataaata attggaggat ttttatataa
     5641 tgatggatta cttaattagt caaaatggtg gaatggtatt tgcggtatta gcgatggcaa
     5701 cagcgacgat tttttcaggg atcggttcag ctaaaggtgt tggaatgaca ggtgaggcag
     5761 cagcagcatt gacaacaagt caacccgaaa aattcgggca ggctctgatt ctgcaattgc
     5821 ttccaggcac gcaaggtttg tatggattcg ttattgcctt tttgattttt atcaacttag
     5881 gaaatgacat gtcagttgta caaggactga actttctagg tgcttctctg ccgattgctt
     5941 ttacaggctt gttttcaggt attgcccaag gaaaagtcgc agcggcaggt attcaaattt
     6001 tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat
     6061 acgcgatttt aggtttcgtt atttccttcc tattagtgtt gaatgcctaa gcataaagga
     6121 ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaacgaag cagcagagca
     6181 ggaaagagcg gcgttagaac aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa
     6241 acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattactcga
     6301 aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa gtacgacaag aaaatttgaa
     6361 tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg gtaactgaaa tggaaaattg
     6421 ggatgaatct gaacaaatcc agtttatcaa aaatgcattg tattcattac ctttgacagg
     6481 aaaagtagca ttcattgctg gagaaaaatc agcagcctat ctttctcaga cgttgttgga
     6541 tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca
     6601 agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt
     6661 acaagatatc caaggaacga tgagttttga gattgccaat caattattcg aataaggagg
     6721 gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt
     6781 ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa
     6841 atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa
     6901 cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag
     6961 cctgaaatcg tttggatcta tacgatgcgt tatactttcc ataatctgaa ggttttgaca
     7021 aaagctgaga tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca
     7081 ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaacttcc tgaaagcatt
     7141 atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt
     7201 gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga
     7261 tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg
     7321 gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg
     7381 ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg
     7441 aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat
     7501 attgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa
     7561 gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta
     7621 gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga atcagaaaca
     7681 atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga
     7741 ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac
     7801 cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga
     7861 gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc
     7921 tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa
     7981 aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt
     8041 gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga
     8101 tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga
     8161 gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg
     8221 cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc
     8281 acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa
     8341 tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga
     8401 accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga
     8461 aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac
     8521 aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg
     8581 tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga
     8641 aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc
     8701 agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt
     8761 tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga
     8821 gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac
     8881 aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc
     8941 agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg
     9001 ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat
     9061 gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg
     9121 gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga
     9181 aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt
     9241 cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca
     9301 aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt
     9361 agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat
     9421 gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc
     9481 cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt
     9541 gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat
     9601 gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta
     9661 tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata
     9721 tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat
     9781 gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg
     9841 aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa
     9901 tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag
     9961 ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg
    10021 tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat
    10081 tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg
    10141 atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa
    10201 caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat
    10261 tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg
    10321 tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag
    10381 aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt
    10441 ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga
    10501 ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca
    10561 tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa
    10621 gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa
    10681 gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg
    10741 ataaaactca tccaatccct gacttgactg gttatattac agaaggacag atcatcttga
    10801 ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc
    10861 ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga
    10921 atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag
    10981 gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa
    11041 aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag
    11101 ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc
    11161 tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga
    11221 cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat
    11281 tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca
    11341 accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg
    11401 caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg
    11461 ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg
    11521 aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg
    11581 aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag
    11641 aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca
    11701 atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga
    11761 agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta
    11821 ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca
    11881 aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt
    11941 agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt
    12001 tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa
    12061 tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg
    12121 cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag
    12181 tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat
    12241 gaagctcatg ctttacatcc taaaattggc ctttgtgatc caattgattg gtacggttgc
    12301 tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt
    12361 ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa
    12421 tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt
    12481 aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca
    12541 ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt
    12601 tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa
    12661 cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca
    12721 tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg
    12781 gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc
    12841 gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt
    12901 gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat
    12961 tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt
    13021 cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat
    13081 agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt
    13141 cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag
    13201 tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa
    13261 ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg
    13321 aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa
    13381 gatttgattc ctttatttat caataggtta taattcatat ttttttattt tttcttttac
    13441 gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa
    13501 tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc
    13561 aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata
    13621 gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc
    13681 tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat
    13741 ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg
    13801 ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca
    13861 aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg
    13921 aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac
    13981 ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg
    14041 aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct
    14101 acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat
    14161 ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc
    14221 tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc
    14281 ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc
    14341 tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag
    14401 atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa
    14461 agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat
    14521 ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc
    14581 tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc
    14641 agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc
    14701 ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg
    14761 tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa
    14821 ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag
    14881 gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga
    14941 agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca
    15001 aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat
    15061 gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga
    15121 taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa
    15181 cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt
    15241 atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg
    15301 agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac
    15361 agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa
    15421 acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc
    15481 ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag
    15541 ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac
    15601 atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag
    15661 cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac
    15721 attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat
    15781 tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa
    15841 attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat
    15901 gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt
    15961 acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa
    16021 aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac
    16081 gcgacattag caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt
    16141 aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg
    16201 gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt
    16261 ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa
    16321 aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga attaggaata
    16381 ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca
    16441 aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat
    16501 tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg
    16561 gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt
    16621 tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga
    16681 gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg
    16741 aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat
    16801 gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa
    16861 gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccagcagc
    16921 tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc
    16981 gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc
    17041 cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct
    17101 ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg
    17161 aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa
    17221 caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa
    17281 tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga
    17341 aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga
    17401 tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat
    17461 tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat
    17521 tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg
    17581 tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg
    17641 agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa
    17701 attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg
    17761 aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt acatattttt
    17821 tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt
    17881 tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca atcatggata
    17941 agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg
    18001 cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg
    18061 gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg
    18121 catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca
    18181 tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat
    18241 aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac
    18301 tgccgacacg gcgtgccatt acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac
    18361 gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt
    18421 aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac
    18481 ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta
    18541 cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta
    18601 gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac
    18661 ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg
    18721 acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta
    18781 atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa
    18841 tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt
    18901 agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag
    18961 tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt
    19021 tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt
    19081 gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc
    19141 ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt
    19201 gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa
    19261 caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg
    19321 catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata
    19381 ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat
    19441 agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt
    19501 aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa
    19561 tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg
    19621 tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg
    19681 ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt
    19741 ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg
    19801 aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt
    19861 taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct
    19921 ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat
    19981 atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg
    20041 atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt
    20101 aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt
    20161 acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca
    20221 ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact
    20281 tgcccacgtt tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt
    20341 ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt
    20401 ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat
    20461 ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt
    20521 tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct
    20581 gaatttctga tcactggagc gtaacctgca tcttccagtg tttggatccc gtcacttaga
    20641 aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt
    20701 aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg
    20761 gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg
    20821 gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt
    20881 tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga
    20941 acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga
    21001 tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg
    21061 atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc
    21121 aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac
    21181 agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag
    21241 aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc
    21301 atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat gatctgttca
    21361 tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa
    21421 agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg
    21481 tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg
    21541 aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta
    21601 accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat
    21661 tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct
    21721 tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag
    21781 aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg
    21841 atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag
    21901 atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt
    21961 ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa
    22021 atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg
    22081 aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga
    22141 gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg
    22201 tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct
    22261 tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa
    22321 aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca
    22381 aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat
    22441 gcaagatagt tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt
    22501 tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa
    22561 aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac
    22621 ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa aagagattct
    22681 gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa
    22741 tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga
    22801 atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa
    22861 cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta
    22921 tcgaagagca atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag
    22981 tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa
    23041 gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct
    23101 ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga
    23161 aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt
    23221 ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag
    23281 attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa
    23341 cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc
    23401 agctctcact tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag
    23461 tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc
    23521 aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt
    23581 atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt
    23641 atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg
    23701 aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta
    23761 agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta
    23821 tttagttatc agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc
    23881 ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt
    23941 caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta acgtcaaaga
    24001 agccattcaa gaatcagcgg aaaaggtaca aagtcagtca tcacaatcaa cacaagacag
    24061 tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca atcagctttg atgatggacc
    24121 aggagcgacc acaacaccgc agcttctgcg aatcttaaaa gaaaaaaacg tgcatgccac
    24181 ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc agacagctga
    24241 agcagggcac gagattggca atcacacata cgatcatcag aatctggcca ctctttctgc
    24301 ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaag tcactggcaa
    24361 aacccccact tttgttcgtc cgccctatgg ttctgtcacg aatgtaggag caacgatcat
    24421 ccagcgaccg atcatcgaat ggtctgttga ttcagaagat tggaagacaa gaaatcctga
    24481 tttgattttg cagaagatcc aagcaacagt atacgatggg gcaattattt tattccatga
    24541 tatctatcct gaaacgattc gtgccgttcc tcaagtgatc gattatctga aagagcaagg
    24601 gtatcgcatc acgacagttg gggatttatt agggcatcca acggctgtcg agaactatta
    24661 tggcagaaac gatcatcggc cagttcagta agtgagacaa gtatctttaa taaataacac
    24721 attttaataa gtagttgaac acataactac cttacttgca aaggcttaac aatatctata
    24781 caggtacaac aaaaaactgt ccaccaattt tctttggcgg acagttttca cttaaaaata
    24841 aagaagcaac tattcgtaaa ttatgctaaa atagcagtag aacgcgtcct gagggaatcg
    24901 aacccccgtc tcaagaaccg gaatcttacg tgatatccac tacactaagg acgcaagcac
    24961 tgggataatt gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat
    25021 ttgagcaaaa tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt
    25081 tgcaacagtc catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga
    25141 atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca
    25201 taaaagcttc ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg
    25261 atacaaaagg gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata
    25321 cttttttgag gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga
    25381 cgattagttt agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg
    25441 cgttgactct gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat
    25501 gtctcatgct tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg
    25561 aagaatgttt tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg
    25621 ttgatctaca tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg
    25681 gccggatttt cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg
    25741 acgacgataa aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc
    25801 aagagagtta cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa
    25861 aagaacggaa acaggaattc gaatggttga agaaaacgat cctacaacgt ggggatacaa
    25921 ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac
    25981 gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca
    26041 cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga cttcggtgta tttgaattaa
    26101 aaaaattttt cactacacga attcctacga atagtacaga acagacagaa gacctttcag
    26161 cagatacagc gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt
    26221 tgtctgacca gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa
    26281 cagtagcaaa atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg
    26341 ataactaatc agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga
    26401 aaaacgagag caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg
    26461 tgaaattcga atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg
    26521 gacgtaaagt gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct
    26581 gttgcaccag atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat
    26641 tggatgcagc cgattggacg tcgaagttta tcggaaagta tggggatcac agaacgagta
    26701 ttgcgaacag aaacagatct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt
    26761 atgacgctga caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag
    26821 cttttaggta cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt
    26881 atcgttgttt caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg
    26941 ctgagcgaag ccctagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga
    27001 ggaactacta tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat
    27061 aacctgtttg tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct
    27121 gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc gtgcacttta tgttccggaa
    27181 caattaagtc tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta
    27241 aacttgatta gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca
    27301 gcacgtcgta agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg
    27361 gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc
    27421 ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa
    27481 tcaaaagcta aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc
    27541 actgatgaag ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt
    27601 tttgattttc ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg
    27661 acgtattgga cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc
    27721 aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca
    27781 aggacgtttc aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga
    27841 agttaaagta ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga
    27901 tatcgttcta gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac
    27961 agctggtgct aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt
    28021 ttataacaca aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg
    28081 tactactaac tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga
    28141 aggattaatg acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca
    28201 tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac
    28261 aggtgctgct aaagctatcg gtttagtaat tcctgaattg aacggtaaat tagacggagc
    28321 tgctcaacgt gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa
    28381 agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg
    28441 atacaacaca gacgaaatcg tttcttcaga tatcgttggt atgacttacg gttcattatt
    28501 tgatgctaca caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc
    28561 ttggtacgac aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc
    28621 taacttataa gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg
    28681 ggaagcaatc gcgcttcttc cgcttttttt gataataagt ttattcagga ggaaataaaa
    28741 tggctaagaa aacagtgaaa gatatcgatt taaaagacaa aaaagttctt gtccgtgttg
    28801 acttcaacgt tcctttgaaa gacggcgtga tcactgacga cactcgtatc aaagcagcat
    28861 tgccaacaat caactacgtt ctagaacaag gcggaaaagc aatccttttc tctcaccttg
    28921 gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt agctccagtt gcaaaacgtc
    28981 taggcgaatt attaggtaaa gaagtaacat tcgtacctga aactcgcgga gaacaattag
    29041 aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt attcgaaaat acacgatttg
    29101 aagatattga cggtaaaaaa gaaagcaaaa atgatacaga actaggtaaa tactgggctt
    29161 cattaggcga tgtatttgtt aacgatgctt tcggtacagc tcaccgtgca catgcttcta
    29221 acgtaggtat cgcttcaact ggtatcccaa cagttgctgg attcttgatg gaaaaagaaa
    29281 tcaagttcat cggtgaagca gtagaagaac caaaacgacc aatgattgca atccttggtg
    29341 gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt gattcctaaa gcagataaaa
    29401 tcttgatcgg tggcggaatg acttacacat tctacgaagc aaaaggaatc aagatcggta
    29461 actctctagt agaagctgat aaagtggaac tagcaaaaga attaatcgaa aaagcaggcg
    29521 acaaacttgt cttgccaatc gacaatgtct gtgcaccaga attttcaaat gatgttgaaa
    29581 cacaagtaat cgaaggagac attcctgatg gtttgatggc tttagacatc ggcccagcat
    29641 cagtaaaatt atttgctgat acattacaag gtgcaaaaac agttgtttgg aacggaccta
    29701 tgggtgtatt tgaaatgagt aactttgcta aaggtacaat cggtgtttgt gaagcaatcg
    29761 ctaacttaga aggcgcaaca acaatcatcg gtggcggaga ctcagctgca gcagcagaac
    29821 aattaggatt tgctgacaaa ttcacacaca tctcaacagg tggtggcgca agcttggaat
    29881 tgcttgaagg taaagaatta ccaggactag cagcaatcaa cgataaataa acgaccaagt
    29941 ttgctcaatt ctgacgagta atcgatcaaa aggtgaaggg agttttcatg actccctagc
    30001 catttttcaa taaattaagg agtgtgttcc tcaatgcgta aacctattat cgcaggtaac
    30061 tggaaaatga acaaaactgc atcagaagca aaagaatttg ctgaagcagt aaaaaacaaa
    30121 atcccttcaa atgacaaagt tgattcagtc atcggatctc ctgcattatt cttacaagaa
    30181 ttaatgacat cagctgaagg tacagaacta aaaatctcag cacaaaactg ctactgggaa
    30241 aactcaggtg cgttcactgg tgaaacttca ccagcagctc ttgctgatct aggtgttcat
    30301 tatgtgatca tcggacattc agaacgtcgt gaatatttcc atgaaacaga cgaagacatc
    30361 aataaaaaag caaaagctat ctttgcaaat ggtatgacac caatcctatg ctgcggcgaa
    30421 tctttagaaa cctatgaagc aggcaaaact gctgaatgga tcgaaggaca aattacagct
    30481 ggtttagctg atctttcagc tgaacaagtg tcaaacatgg ttattgctta tgaaccaatc
    30541 tgggcaatcg gtactggtaa atcagctgac gcacaaatcg ctgacgaaat ttgtggagtt
    30601 gtacgtcaaa cagttgaaaa actttatgga aaagaagtat cagaagcagt tcgtatccaa
    30661 tacggcggtt ctgtaaaacc agaaaatatt gctgaataca tggcaaaaga aaatgttgac
    30721 ggtgctttag tcggtggcgc aagcttgcaa gcagattcat ttttgtcatt gttagacgct
    30781 gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat gtttgcacta atgagcaaaa
    30841 ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa acaaactcaa aggagagaca
    30901 aaacatgtca attattactg atgtttacgc tcgcgagatc ttagactcac gtggtaaccc
    30961 aacaatcgaa gtagaagtat acacagaaag cggagctttt ggccgcggaa tggttccttc
    31021 aggtgcttca actggtgaat acgaagcagt tgaattacgt gacggagaca aagctcgtta
    31081 tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac aacatcattg ctgaagcaat
    31141 cattggctac gatgttcgtg accaaatggc tatcgacaaa gctatgatcg ctttagacgg
    31201 aactcctaac aaaggtaaat taggtgcgaa cgctatttta ggtgtttcta tcgctgttgc
    31261 acgtgcagct gctgactacc tagaagtacc tttataccac tacctaggcg gattcaacac
    31321 aaaagtattg ccaacaccaa tgatgaacat catcaacggc ggatcacatg ctgataactc
    31381 aatcgacttc caagaattca tgatcatgcc tgttggcgct ccaacattca aagaagctct
    31441 acgtatgggt gctgaagtat tccacgcatt agcttcaatc ttgaaagctc gcggattagc
    31501 aacttctgtt ggtgacgaag gtggatttgc tccaaacctt ggttcaaacg aagaaggttt
    31561 tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta cctggtaaag acgttgttct
    31621 tgctatggat gctgcttctt cagaattcta cgacaaagaa aaaggtgttt acgtactagc
    31681 tgactcaggc gaaggcgaaa aaacaactga cgaaatgatc aaattctacg aagaattagt
    31741 ttctaaatat ccaatcatct caatcgaaga tggattagac gaaaacgact gggacggatt
    31801 caagaaatta actgacgtat taggcgacaa agttcaatta gttggtgacg acttgttcgt
    31861 aacaaacact caaaaattat ctgaaggtat cgaaaaagga attgctaact caatccttat
    31921 caaagtgaac caaatcggta cattaacaga aactttcgaa gctatcgaaa tggctaaaga
    31981 agctggctac acagcagttg tatctcaccg ttctggtgaa acagaagatt caacaatctc
    32041 tgatatcgct gttgcaacaa atgctggcca aatcaaaact ggttctctat cacgtactga
    32101 ccgtattgct aaatacaacc aattactacg tatcgaagac caacttggtg aagttgcaga
    32161 atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa taattctttt ctaatgaaga
    32221 tacatgatcg actattgaaa gatagtcgag tatgatcact tagttaagta atcgaactct
    32281 ctacttgctg aaatatcagc aggtagaga
//
LOCUS       DACCVU010000023        31251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCVU010000023 DACCVU010000000
VERSION     DACCVU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.023"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            14..3292
                     /locus_tag="IVW29_000913"
     CDS             14..3292
                     /locus_tag="IVW29_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7838412.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3833..5740)
                     /locus_tag="IVW29_000914"
     CDS             complement(3833..5740)
                     /locus_tag="IVW29_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7838413.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5870..6745
                     /locus_tag="IVW29_000915"
     CDS             5870..6745
                     /locus_tag="IVW29_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7838414.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6825..7799
                     /locus_tag="IVW29_000916"
     CDS             6825..7799
                     /locus_tag="IVW29_000916"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7838415.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9124
                     /locus_tag="IVW29_000917"
     CDS             7988..9124
                     /locus_tag="IVW29_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7838416.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9261..9605
                     /locus_tag="IVW29_000918"
     CDS             9261..9605
                     /locus_tag="IVW29_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7838417.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9876..11162
                     /gene="efmA"
                     /locus_tag="IVW29_000919"
     CDS             9876..11162
                     /gene="efmA"
                     /locus_tag="IVW29_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7838418.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11300..11899)
                     /locus_tag="IVW29_000920"
     CDS             complement(11300..11899)
                     /locus_tag="IVW29_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7838419.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12303..13424
                     /gene="mnmA"
                     /locus_tag="IVW29_000921"
     CDS             12303..13424
                     /gene="mnmA"
                     /locus_tag="IVW29_000921"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7838420.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13670..15091
                     /gene="arcD"
                     /locus_tag="IVW29_000922"
     CDS             13670..15091
                     /gene="arcD"
                     /locus_tag="IVW29_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7838421.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15105..16223
                     /locus_tag="IVW29_000923"
     CDS             15105..16223
                     /locus_tag="IVW29_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7838422.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16283..17575)
                     /locus_tag="IVW29_000924"
     CDS             complement(16283..17575)
                     /locus_tag="IVW29_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7838423.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17725..18285
                     /locus_tag="IVW29_000925"
     CDS             17725..18285
                     /locus_tag="IVW29_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838424.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18414..18791
                     /locus_tag="IVW29_000926"
     CDS             18414..18791
                     /locus_tag="IVW29_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7838425.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18724..20796
                     /locus_tag="IVW29_000927"
     CDS             18724..20796
                     /locus_tag="IVW29_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7838426.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21007..21954)
                     /locus_tag="IVW29_000928"
     CDS             complement(21007..21954)
                     /locus_tag="IVW29_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7838427.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22200..22664
                     /locus_tag="IVW29_000929"
     CDS             22200..22664
                     /locus_tag="IVW29_000929"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7838428.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22726..23769)
                     /locus_tag="IVW29_000930"
     CDS             complement(22726..23769)
                     /locus_tag="IVW29_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7838429.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24146..25111)
                     /locus_tag="IVW29_000931"
     CDS             complement(24146..25111)
                     /locus_tag="IVW29_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7838430.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25345..26190
                     /locus_tag="IVW29_000932"
     CDS             25345..26190
                     /locus_tag="IVW29_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7838431.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26381..27568
                     /locus_tag="IVW29_000933"
     CDS             26381..27568
                     /locus_tag="IVW29_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7838432.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27574..27930
                     /locus_tag="IVW29_000934"
     CDS             27574..27930
                     /locus_tag="IVW29_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7838433.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27932..28270
                     /locus_tag="IVW29_000935"
     CDS             27932..28270
                     /locus_tag="IVW29_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7838434.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            28665..29699
                     /locus_tag="IVW29_000936"
     CDS             28665..29699
                     /locus_tag="IVW29_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7838435.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29692..30378
                     /locus_tag="IVW29_000937"
     CDS             29692..30378
                     /locus_tag="IVW29_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838436.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30402..31250
                     /locus_tag="IVW29_000938"
     CDS             30402..31250
                     /locus_tag="IVW29_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7838437.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT        10190 a         5707 c         6649 g         8705 t
ORIGIN      
        1 gggaggaata cacttgttaa aaaaacgtat aaaatacagt gcgctaatga tgaatctcct
       61 tatgctcagt accccggttt taatttctgt cgttgaagtt gctgctaacg aacaacagtc
      121 tgtgaacgaa gaaaatgcac acgtgaacat tgaagggatg tcaacaatgg aggaaatacc
      181 aagaactagt gaagatatgg atacggaaga aggaaatgat cctatgatga aagaatcaga
      241 aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa gagtggaaag gggttgtttt
      301 cggagaaagc acaagtgcta gtaggtatgc cattgaacca ttagaagacg gcgtgcggct
      361 ctcttccacg aataacggcg gaaaattcca gtcaagcgga tcagatggaa tgacctatta
      421 ttatcaagca atccccaaag acatcaactt taggatgcgg gcgacgatcg aaatagaatc
      481 ttggacctat acgaatgccc aagaaggatt tgctttaatg gtaagagacg cagtaccaaa
      541 aaatcatttg tttgggcagg cattttattc aaattcattt gccatactag gcagtcggat
      601 cgaatatgtc tgggactcgg atagacaaga agtggtcaac acgagcggca gcaaatatac
      661 gatgcgactt ggattgggga cacgggcaat cacaggaata tcatctgaag atccgcaagc
      721 tgcgccagct cctgggagtg tctcagtgga aacaacacct ttagagactt cagcagggtt
      781 cttggaaaaa gaaacaggtt cttataatat attcggaaac ggtcatggga atctgtttcc
      841 ggccacgaac tcgattacta aactggacgt agagatgaaa aaaacgaata ctggtttaga
      901 ggcatattat tacgatcctg aaacaggaga agaaatggct tctgacatca tgtatgactg
      961 ggaaaaatta tacgcttcag atgaatcggt tatttatgca ggatttgctg cagcaaggaa
     1021 tatgacgatc aaggtagaag gaatcgaagt tgcttactca gatcctgcga cagatcctcc
     1081 tgggcaagaa agacctactc gcttgattga tccgatttac actgtgacct ctagtaatca
     1141 ttcaggaaac caagatcata ctatgtcctt tagagcaaat gctgacggat tattgacaat
     1201 taaaaataaa gcaacaggag aggtgcttaa gaacgcaaaa aacttggcta tcactgcgaa
     1261 tcgggaattt gattatcaga caaaactgat ggaaggaacg aacgaattta ctttcagttt
     1321 tacgccagat aaaaactatc cgccagaaga atacgtagag atgactagtt atgaaacaaa
     1381 agagattctt catacagtgg actaccgtaa acctaaatat tcgacagttt atgtaacacc
     1441 agaagggtca gatgcaggaa acggaagcag acaaaatcca ttatctctga cacaagcatt
     1501 gagatacgca tatgctgggc agacaatcgt catggcgcct ggaacttatt ccttcgaaag
     1561 aggattgacg attccaaaag gcgttaacgg gacaagagaa aacccgattc agttgattgc
     1621 agagaaaaat ccagaaaaca agcgtcctgt acttgatttt aaaggaactg gaaatgggat
     1681 gacactgttc agtcactact ggggtttacg agggtttgac attacaaata gtgcagctat
     1741 gcaaaaagga ttacagattt ctggaaatca taatttgatc gaagagattc atgcttatcg
     1801 gaatggaaat acaggaatcc aaatcagcgg tagctcaaat gatcctttcg aagcatggcc
     1861 cgcgcataac ttagtcaaga actgtacatc ttttgaaaat gccgatccag gatttgaaga
     1921 tgccgatgga tttgcggcca aactgacaat aggagaagga aatgtatttg acggttgtat
     1981 cgcgtatcat aatgcagatg acggttggga cctttttgct aaaaatgaaa caggttcgat
     2041 cggaaaagta atcatcaaaa actcagttgc ttatcgaaac ggctgggtac caggaattga
     2101 gggagaaggt aacggtaatg gattcaaaat gggcggttct tctttaacgg gccctcatga
     2161 attgatcaat agtttgtctt tcgaaaatct tgcgaaagga atcgattcca atagcggaac
     2221 agatattatc gtcaatagca gtacaagttt taataacggt tcatataatg tggctcttta
     2281 tacctcttca gcaggaaaca cagactacca tgcttctggt atcttatctt tccgaacaga
     2341 gaatcttgag atgagagagc agatccgccc attgaatcaa aacgaagatc aaatctacca
     2401 ctcgttgaac tactattgga atgaacgaga gaaacaatca gaaaatactc ttggacatac
     2461 catatcaaaa gattggatcg agtctctaaa tacatgttcg aaagaaggtc agcagcctgt
     2521 tacgcgctat gaaaatggaa gtatccatgt tcatggactg atgcagatta aaccggggaa
     2581 tcgtcaaaca gaaaaggaaa gagtggatgg tcttccatta tctgtaggtg ctatttttga
     2641 cggagaaacc tcaccgtctt cggaatatcc agagtatgca atcgtacaag aaccaaatca
     2701 gacaaaacca aatgaacaaa ataatccgat tattatacaa gaagcaaatg gtgtggcgga
     2761 gaatgcagaa ggaaatatca ttgaactgaa gccttttaca gtttttgtgt caactttcac
     2821 accaaatcaa ggaacgcctg tctttgaaga tatgcgttat ttcccagaaa agatccaaat
     2881 cgttgaaaca cagtcaagtg ctcaattttc taatcatacc gctatttttt cagcaccttc
     2941 aaaaggagaa tatacgctag aagtcactta tcgttcggaa atatttgatg gggaaaaatg
     3001 gtctgctcat acagaatctg ttacccagca aaaagcgatc actgtgcgag aaactgccga
     3061 tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca ggaaatgaat caccaaatgg
     3121 aaaaccaaat ggaaaacctt ctcatccgga taggacagca aaagaaagtg aaaaaaatgg
     3181 cactcaagga ctcccaaaaa cgggagaatt taataacccg ttgctagcat tagctggagg
     3241 aattttgtta atcggtgtag ttgtttatgt catgaaacag cgaaaaaaat aagtggaatt
     3301 tctcctttta tgatgtaaaa caattgactg aggaaagtaa aaatagaagg aaatgagacc
     3361 aatagatgtc tcacttcctt ctatcttttt atagatgagg ttctccttgc tgcttttgat
     3421 tggctgttct tattacttga tgcaggacag ctttttcaca atctctccac taaacggtgt
     3481 tcaatcagct gaccctcggt attagctcaa caaacggcaa aatatgagga actatttttc
     3541 cgtttttttc tctaatactc aggtcaggac gctgatttca caacctctcg ataaacggtg
     3601 ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga aagccatttt
     3661 cacaaaattt ttcttactac ttgaaactaa gcagcttttt cacaacctct aatacatggt
     3721 attccagaag taacttcttc gaaaaataag ccgaaatgtc acaaaaattt gagaagcgat
     3781 tttcccaaat tccttcttat ttcttgaagc tgaccacttc tggcacaacc tcttaccaga
     3841 ttcgcactct tttttctggc gctagataca tagcgtcttg ttccgtgata ccaaatgttt
     3901 catagaattc gtcaaggttt tttggttgca cgtttgctcg gagtttagca ggggcatgga
     3961 catcgatttg cagtaaaagt tgttggtatt ctttttttgc ttttgtacgc caaatcgttg
     4021 cccaattgac aaagaattcc tcgatagaaa gatctgattc cgtttttgct gcttccaatg
     4081 cacagcttag accgcctgca tcagcaatat tttccgaaac ggttaatttt ccgtttactt
     4141 ttccgtcagc aaaaggcaga ccatcgaatt cttcgatcat tgcttgtgct ttgttttgaa
     4201 attgtgtaag atcttcttct gtccaccagt tgttcaagtt gccatattcg tcaaataaag
     4261 cgccgttatt atcaaatgca tgggaaatct catgagcgat aacagctccg attccaccat
     4321 aattagcaga gcttgattgt tctagactgt aaaatggtgc ttgaaggatc gctgccggaa
     4381 agacgatcac attccgaaat ggatgataat aagcattgac cgtgtcagca ctcatttccc
     4441 attctgaacg atccactggt ttgttccatt tgctgaaacg atcttttaat gtgagtttgc
     4501 tgaaaacaag agtatttgaa agcaaagtac caccctgatc tgcaggtact gttttgaact
     4561 ggtcgaataa tgctgggatc ttatcggggt agcctacttg gatacctaag ttatttaatt
     4621 ttatgacggc tttttctttt gttgcatccc caagccagtc attttcttct agacgttttt
     4681 tataaacggc gatcattttt ttcaccattt gttctacgtc ttttttagca gcttcgccaa
     4741 aatatttttt tccgtagtaa tcgccaacta cttgatcgaa ttgtccagag gcaaggtaat
     4801 acgctgcttt tttctgaggc attgcttcgt ctgttccaga caatacgcga gaatagattc
     4861 cgctgacttg gcggaattct tctgatagat aaccactaag agaacgtact gttttaatga
     4921 tcatccagct tttgaataac ggaaaatgag cttctgtcag gatgtcattc aaatgatcga
     4981 agtacactgg atcagttacg atcactttat ctggagttgt cccaatcaaa cccgtgacaa
     5041 gttttttcaa gtccagttta tctgtgtatt taataaatgt atcaaaagat tgcggattat
     5101 acattttgct gtaatctgcg ttttcttctg cactttttac atgaggagca atcaatttat
     5161 caaattgcac tgcttgttcc gtgattgctt ctgcctgact tttttctttg ccggcaagct
     5221 ctaataactg aacagccata tcaaagaaaa cgcctattag ttgtgcaccg ttgggatggt
     5281 ctgatgcata atacgtctta tctggaagga ataaagaagg cggataagca aagaaggcgt
     5341 tcgtttgcgc attcttcata tctgcatcta catccagatt aaatggcaat ggtaatgtat
     5401 ccaacagcca ttctggaaat tgcttattca attcgtcgaa agactgcaat gattcaactt
     5461 tttcgattat aggttgaagc ggttgagcat gcaactcatt tcttcggtca taatcgtttg
     5521 ccagttgata atatgcaagg aaatgtttca ttttttctga agacactttt tctggatggg
     5581 caagcagatc gtctgtttct cccatcaaaa gttggtcgat cccgtcaact aaatcttgga
     5641 aaccgcctgt agctggttta tctgcaggga tttttgctgt tttttgccat tcttcattga
     5701 ctgcttcata gaaatcttgt ttcaatcgtt ctttgttcat tcatcgatcg ctcctttttt
     5761 gtttcgtgtg cccattatct tacaaaatga aggaattaac aaatagaaca taaaaaattg
     5821 aaaattcgaa caatcaaact gatttgtttt tttcaatcct tcacttataa tgaaaaaaag
     5881 gaggcgatcc caactgaatc aagtagtgga taaaccttta tatttcaaag tagataaaaa
     5941 aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat tgcttttttt
     6001 agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac atccggtaga
     6061 atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat ggaagctgat
     6121 caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata ttcccgtttt
     6181 gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat tgccagaaga
     6241 acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg gtacttacgc
     6301 ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc ttccaaatga
     6361 gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc gtagccagat
     6421 ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt ctagtttttt
     6481 aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat ggcagcgagc
     6541 tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag tacttggcag
     6601 tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa caggagaacg
     6661 ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg ccagttggag
     6721 taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat cttggaaaaa
     6781 tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt aataagtatc
     6841 aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct gaaaaaatcg
     6901 cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt gatggcgaga
     6961 tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt caatcaacga
     7021 atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca atgaagcgag
     7081 caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg caagaccgca
     7141 cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc gaagaagcag
     7201 gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga ttctttgata
     7261 ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt caattaggtc
     7321 tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag cgtgcacgta
     7381 gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat gatgcttcaa
     7441 ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg gtagatgata
     7501 tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa aacggcgcaa
     7561 aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa agtattttag
     7621 acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa gaaaatcgtc
     7681 atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg gtcaaacgca
     7741 tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa ttcgaataaa
     7801 caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag ctgcgacaag
     7861 acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca aaaatgagct
     7921 cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt tggaagggat
     7981 gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc atccacaagt
     8041 aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa gcatacatgg
     8101 atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg cagacagtct
     8161 gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg ataatacagc
     8221 tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca ctacagctat
     8281 cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt ttgacgttac
     8341 atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa aagcgttacg
     8401 agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa atttgatgcc
     8461 aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata cagatgcggt
     8521 acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt tattaagtat
     8581 ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac gagacggagt
     8641 aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg caggtacaga
     8701 aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac ctgaagaacg
     8761 tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata cattagaagc
     8821 aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc atgtgttgaa
     8881 tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc ttaatggggt
     8941 cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac acgtattaga
     9001 agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca gctttggttt
     9061 gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg ctgtcaaaaa
     9121 ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt cattttttag
     9181 ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa agagaaacta
     9241 attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg ggcgcaccaa
     9301 ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat ggatttacag
     9361 aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg caaagcaaag
     9421 aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa atgtcgatca
     9481 ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag aaaatgagcc
     9541 aagataaatt ttattttctg atggatggga tgattagccg tgggttattc gaaaaagtcg
     9601 aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt agatgactaa
     9661 ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac acatgttgtt
     9721 atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt gaaacgattg
     9781 ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt gttgtgcaaa
     9841 cgctatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag tcggtcgttt
     9901 taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg tcaggaatca
     9961 ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc ggttcggcga
    10021 caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta agcccatttg
    10081 taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg gatattattg
    10141 tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc ttcccgattt
    10201 ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa atgccaacga
    10261 tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg aatggacagt
    10321 taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg gctttgtttt
    10381 cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt tttggtgtcg
    10441 gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa gttccgctta
    10501 ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa gggttatggt
    10561 acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc agtctgtatc
    10621 cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca gtggaagtag
    10681 tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga acatggaaag
    10741 accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc ggtgcaagcg
    10801 gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat gcaggagcag
    10861 gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag agttatgaat
    10921 cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg gcaggtccaa
    10981 ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa atgttcttgt
    11041 tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca gctgcccgaa
    11101 attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa aaagacgaat
    11161 agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat cctaatgttc
    11221 ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt tgtttaatac
    11281 ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca tagatttctt
    11341 tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag agttctcttt
    11401 ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa tgatggatat
    11461 gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa tgagtcaagt
    11521 aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa aatttgggac
    11581 ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg cgactgtcat
    11641 ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc aaaatttctg
    11701 catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa acatggtcgg
    11761 tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca tccacatttg
    11821 caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa aagtgcatat
    11881 cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta aacaaaatca
    11941 cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac gtgcagcttt
    12001 aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt attccttatt
    12061 gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg ctcaaaagat
    12121 gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta gaacagtatt
    12181 tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa tgtaaaatac
    12241 tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat gatggtgata
    12301 tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt gtcgactcat
    12361 ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata ttcatgaaaa
    12421 attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat aaggatgtag
    12481 cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa aaagaatatt
    12541 gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca cctaacccag
    12601 acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca atggatcttg
    12661 gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa aacggcgtgg
    12721 tccacatgct tcgtggagta gacaataata aagatcaaac ttacttcctt agccaattat
    12781 cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa tcagaagtac
    12841 gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca acgggaatct
    12901 gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca gcaaaaaaag
    12961 gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg atgtattata
    13021 cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa gaaccctggt
    13081 ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg ttccatcatc
    13141 ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg aatgaaccaa
    13201 tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac gttcctgtga
    13261 cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct gtacgtgcaa
    13321 tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt ggtggtttga
    13381 tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata ttaaatgaat
    13441 caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata gctgtttttt
    13501 tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa aagttcataa
    13561 tatccaataa tatgaataaa gttgcttttt gttaagcaag agcagttgca aaatagaagg
    13621 gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa tggaaaagaa
    13681 atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt caatcggaag
    13741 cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc cagcgatcct
    13801 ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga atcatttagg
    13861 agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt catttggtaa
    13921 attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg gaaatgttgc
    13981 ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca aaggtggaca
    14041 aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt tattagtaaa
    14101 caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta aattagtgcc
    14161 attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat tcactgctga
    14221 tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag gaacgatctg
    14281 gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga tcgaaggcgc
    14341 aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat cgatcattgg
    14401 tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg gcgtcttatc
    14461 tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa aaggaatcgt
    14521 gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca ttggttcttg
    14581 gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag acggtctgtt
    14641 gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt tagttgttac
    14701 tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg cctatcaatt
    14761 tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg ggatctatca
    14821 agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg ggattgctgt
    14881 agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt tatgtagtat
    14941 tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca aacatcaaat
    15001 tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc tttcaatcgg
    15061 gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa atcgcaaatt
    15121 tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat attgcacaaa
    15181 gctcgatcga agcattgaca ttagacgaac tcgttggctt agatgggact agcgtaagta
    15241 aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa ggatcagatg
    15301 ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac atcttacaaa
    15361 cgaatggagc gactggtgct aatcttttag ctctatacgc gttagtggaa gcaggagacc
    15421 atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa tctttaggtg
    15481 ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat ttagaacagc
    15541 tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca aacaatccta
    15601 caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct cgtacagtag
    15661 atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt gaattcttgt
    15721 cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa acctactcca
    15781 ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa gtttttcgaa
    15841 agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt gctgtacatg
    15901 cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca gagaaccttg
    15961 tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct cccaattacg
    16021 tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt tgtatcaatt
    16081 tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta ccgaagcatg
    16141 cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc ctcttatctg
    16201 agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg acaactgttc
    16261 aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct gaagctatgg
    16321 agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt gtattgtgtc
    16381 gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg ccgcgataac
    16441 caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca gttgcttgga
    16501 aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat tttgtgatca
    16561 atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa cgtaaggcag
    16621 ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc gcaaaaccca
    16681 aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca aatcttggag
    16741 cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc aacgtaacat
    16801 tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta atggaattgt
    16861 tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt ggatatttca
    16921 agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg acaaattttt
    16981 catggacgaa aaacagactc aacaagaaga cgaaaaataa gatgatccct gtaatgaaaa
    17041 aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt ccaagtaatg
    17101 ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt tcttttggcg
    17161 tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc ccttgaagca
    17221 gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa atgacgattg
    17281 ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg gctaattttc
    17341 cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc aatccgctcc
    17401 aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt gacataaaag
    17461 gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag acaaacaagt
    17521 tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca gccaattaga
    17581 ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa tatgtagttt
    17641 atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga gtgaaggatt
    17701 tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga gaaaatcatc
    17761 gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt tcagcattta
    17821 gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa tattcgtgat
    17881 gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct gcgacgggct
    17941 cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta tcgatccttt
    18001 gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac caatgaagct
    18061 gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt tgaggctttt
    18121 caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat gatccacggt
    18181 tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga tccagaagac
    18241 agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat cagattcatt
    18301 tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt cttgcaactt
    18361 tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg agtatgtatc
    18421 aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac tgggaagaag
    18481 atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg tacgaaaaac
    18541 aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac tttctcagcg
    18601 ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat ttacagcaat
    18661 acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc agaaaggaat
    18721 tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct aagcaaggtg
    18781 cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat cgttacttgg
    18841 gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga atggcagagc
    18901 tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc acttcctaaa
    18961 acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt ctcagaagca
    19021 gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat cttagagtat
    19081 gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga taaagcaatc
    19141 aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta tgaatctgct
    19201 cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc cttaactgtg
    19261 aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc attagtgaga
    19321 gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat cacgttgcaa
    19381 gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa aacacaagaa
    19441 gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac agaagtccaa
    19501 gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat ccaaagagaa
    19561 gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca aagtttgtat
    19621 gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac aaatgacatg
    19681 aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga ggtcaagcag
    19741 tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag taaagtgacc
    19801 gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt aagtaatgaa
    19861 gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc tcaaccccat
    19921 aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat ttttcggcaa
    19981 aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga aacagtcaaa
    20041 tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact atctgcaccg
    20101 cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag tctgggaacc
    20161 ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat ggaacgtacc
    20221 cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt aaatccctct
    20281 ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat tgctgcagga
    20341 aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg cttggtgaat
    20401 gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa gaaatgccca
    20461 gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta tgtctgcacc
    20521 aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca tcgatgcccg
    20581 caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt ccgttgtaaa
    20641 tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa aatgaccaaa
    20701 catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga agaaagccca
    20761 ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga ggttgtgata
    20821 gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt
    20881 gaaatttcgg cttatttccg aagaagctac ttctggaaca ccatttattc ggaaaaaagg
    20941 actgtgacaa gacttttgtc acagtccttt ttcatttaaa aaaatgttta cttcatttga
    21001 cgtatattat tggtgaggct tcaattcatt tggcttcact ttttctgttt ctcttatgat
    21061 ataaacagga cgctgtttcg tttctaagaa gattttccca atatatttcc cgatgattcc
    21121 aagacataac aattggattc caccaatgaa caggaagatc gagaccatgg aaggccatcc
    21181 tgatgtagga tcaccaaaca acaatgcccg caaaataatg aacaaaattg caataccaga
    21241 accgacacaa gacaaagcgc cgataaatga agcaatcgtt aacggtgcat ccgagaagtt
    21301 cacgatacca tcaatcgagt agttgaataa tttccagaag gaccaagatg tttcgccagc
    21361 cacacgttcg cggttttcat aagaaatata ttctgtttta aatccgaccc agctaaataa
    21421 tccttttgaa aatcggttgt attctgttaa ttctaggacg gcatccacca tttgacgagt
    21481 catcaagcgg aaatcacgag cgccatcgac catttctgta tcgctgattt tattgatcaa
    21541 ctggtagaat tttttcgcaa agaaactgcg tatcgcaggc tcgccttcac gagtcgaacg
    21601 ccgggtgccg acacaatcga tatcagaagt agtgatccgg ttgatcatct caggaagcag
    21661 ttcgggcgga tcttgcagat caacatccat cacggtcact aaatctcctg tagaagcttg
    21721 caatccagcg tacagagccg cttctttccc aaaatttctg gaaaatgaga caaagcggac
    21781 ataaggataa gctgaggcta gcttcttgat tgctgctaac gtaccgtctt ttgatccgtc
    21841 gtcaataaac aaatattcga actcatgggc acctttgaat ttttcaacct catcaaaaaa
    21901 aagaggaaca gttttttctt cattaaaaca tgggacaaca attgatactt tcatcgtttc
    21961 tatcctttca atcgtatgtt tatatatggt ttacattcaa aatttaataa accttaaata
    22021 acacctttaa aatttgattt tacgtaaaag aacaggtatt ctttttgtaa actaacgtat
    22081 ttagtttacc atgtttcctc ggtttatcaa acttttaaga acaattttta ttagttggca
    22141 agtagttttg gatagagaaa aaggaggaag ttccagtcta ttgactagga gcaaacaaaa
    22201 tgaaaataca cacacctatc ggctttttat ggttagatgc agatgaatcc ggattgactg
    22261 ctgtatcttt tcagccaatt tcagaaagcg aatcagcgaa tcagcgaatt ttaacgcgca
    22321 cacaagaaca gtttgaagaa tattttgcgg gaaaaagaaa agaatttgat gttcctttat
    22381 ctataaagag aggtacgcct tttcagcaaa atgtctggaa agctttgtcg accattcctt
    22441 atggagaaat ccgaacgtat aaagaagtag ccgttgctgt tgatagtccg aaagctgtgc
    22501 gagcgattgg gcaggcaaat cgaaccaatc cattgccaat cgttattcct tgtcatcgag
    22561 tgatcgggca aaatggaaaa ctgacaggat atatgggaaa cgcaatagat ggggttgaaa
    22621 tcaaacgcca attgttgttg ttagaaggat atctgcaaaa ttaaaaacag ttgtgattcg
    22681 gacattgttc catgcgagaa tcacaactgt tttttcatgt tcattctaaa ctttgatcag
    22741 ccaaataaat agtaaagcca tcactgtttg gagaagcccg acagctaatc cataagataa
    22801 gaagcgtgta ccttctttca tgaattttgc aaagtctaaa cgcagaccaa ttgccgctaa
    22861 agcagtgatc tcaaaccagc tgctgataaa atgtgccgat tggtcaataa ctacaggtaa
    22921 agaaaacaag ctgttgacca aacaaaagat cacaaaccct aatacatacc aaggaagcgg
    22981 taattttcgt tttttctgcg tatcagttgg cgtttcaaac agagctggtt gtttcttgtg
    23041 catgaatttt tcaaacagat agacaacaac aacgagcatc ataatacgta gaatcttaaa
    23101 tagcatggcg aattcgacaa cagaattgtc aagtaagctt gccccagcga ctacttgacc
    23161 aacagattgg agtgtaccac ccaaaagtgc acttttttca atgagatttc caccaaacaa
    23221 ctgtaatcca agaaaaggga gagtcaacat catcacagta cccagtagat tgactagtgt
    23281 gatgatctgt cctttttctt cctctttcgc atggatggaa ggggcgattg aagcaatagc
    23341 agaagaacca caaacagcgt ttcctccagc catcatcaaa gaagaagtat cgctgaattg
    23401 cagtttttta ccaatcaagt aagcaccgat gatagtgact gacatttgca gtaacacaaa
    23461 aacaattcct tttacaccaa tttgagcaat cgtttgagct gtgacagttg cacctagtaa
    23521 aaaaacagag aattctaata gtttgctttc agcaaatttt gtgccgattg ctagaacagg
    23581 ctgtttaaaa aaagtatttc ctaataaaat acccaataaa atagcaatag tcgctccacc
    23641 taactgtggc agataaatag caagaagcct gctgaaggca gcgatggcaa aagaaagcag
    23701 taaaccgggt aagatttcgc ggaccgctga atcctttaca gattttattg gatgtgacaa
    23761 tgatttcatt tgatcggttc ctttcgtcaa taaatactaa aagcatactc gaacatagta
    23821 gcactaaacc agtattttga caaaagaaca agaggtcgtg acaaaagtct tgtacgacct
    23881 cttgtttcga ataaacggtg ttccagaagt agcttctacg gaaataagcc gaaatttcac
    23941 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctt
    24001 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
    24061 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
    24121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
    24181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
    24241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
    24301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
    24361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
    24421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
    24481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
    24541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
    24601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
    24661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
    24721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
    24781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
    24841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
    24901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
    24961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
    25021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
    25081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
    25141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
    25201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
    25261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
    25321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
    25381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
    25441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
    25501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
    25561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
    25621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
    25681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
    25741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
    25801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
    25861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
    25921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
    25981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
    26041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
    26101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
    26161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
    26221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
    26281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
    26341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
    26401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
    26461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
    26521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
    26581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
    26641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
    26701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
    26761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
    26821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
    26881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
    26941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
    27001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
    27061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
    27121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
    27181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
    27241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
    27301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
    27361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
    27421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
    27481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
    27541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
    27601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
    27661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
    27721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
    27781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
    27841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
    27901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
    27961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
    28021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
    28081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
    28141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
    28201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
    28261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
    28321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
    28381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
    28441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
    28501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
    28561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
    28621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
    28681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
    28741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
    28801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
    28861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
    28921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
    28981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
    29041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
    29101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
    29161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
    29221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
    29281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
    29341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
    29401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
    29461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
    29521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
    29581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
    29641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
    29701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
    29761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
    29821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
    29881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
    29941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
    30001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
    30061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
    30121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
    30181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
    30241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
    30301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
    30361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
    30421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
    30481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
    30541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
    30601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
    30661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
    30721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
    30781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
    30841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
    30901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
    30961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
    31021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
    31081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
    31141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
    31201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa g
//
LOCUS       DACCVU010000024        30860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCVU010000024 DACCVU010000000
VERSION     DACCVU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.024"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(35..2023)
                     /gene="treP"
                     /locus_tag="IVW29_000939"
     CDS             complement(35..2023)
                     /gene="treP"
                     /locus_tag="IVW29_000939"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7838438.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2246..2959
                     /gene="treR"
                     /locus_tag="IVW29_000940"
     CDS             2246..2959
                     /gene="treR"
                     /locus_tag="IVW29_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7838439.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3036..3482
                     /gene="msrB"
                     /locus_tag="IVW29_000941"
     CDS             3036..3482
                     /gene="msrB"
                     /locus_tag="IVW29_000941"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7838440.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3652..4254
                     /gene="ruvA"
                     /locus_tag="IVW29_000942"
     CDS             3652..4254
                     /gene="ruvA"
                     /locus_tag="IVW29_000942"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7838441.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4267..5268
                     /gene="ruvB"
                     /locus_tag="IVW29_000943"
     CDS             4267..5268
                     /gene="ruvB"
                     /locus_tag="IVW29_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7838442.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5297..5875
                     /locus_tag="IVW29_000944"
     CDS             5297..5875
                     /locus_tag="IVW29_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7838443.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5927..6409
                     /locus_tag="IVW29_000945"
     CDS             5927..6409
                     /locus_tag="IVW29_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7838444.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6526..6900
                     /locus_tag="IVW29_000946"
     CDS             6526..6900
                     /locus_tag="IVW29_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838445.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7578..8930)
                     /gene="brnQ"
                     /locus_tag="IVW29_000947"
     CDS             complement(7578..8930)
                     /gene="brnQ"
                     /locus_tag="IVW29_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7838446.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9077..9667
                     /locus_tag="IVW29_000948"
     CDS             9077..9667
                     /locus_tag="IVW29_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7838447.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9795..11144)
                     /locus_tag="IVW29_000949"
     CDS             complement(9795..11144)
                     /locus_tag="IVW29_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838448.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11312..12052)
                     /locus_tag="IVW29_000950"
     CDS             complement(11312..12052)
                     /locus_tag="IVW29_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7838449.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12065..13657)
                     /locus_tag="IVW29_000951"
     CDS             complement(12065..13657)
                     /locus_tag="IVW29_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7838450.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14225..14521)
                     /locus_tag="IVW29_000952"
     CDS             complement(14225..14521)
                     /locus_tag="IVW29_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838451.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14697..15422
                     /locus_tag="IVW29_000953"
     CDS             14697..15422
                     /locus_tag="IVW29_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7838452.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15415..16257
                     /locus_tag="IVW29_000954"
     CDS             15415..16257
                     /locus_tag="IVW29_000954"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7838453.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16260..16781
                     /locus_tag="IVW29_000955"
     CDS             16260..16781
                     /locus_tag="IVW29_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7838454.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17034..17597)
                     /locus_tag="IVW29_000956"
     CDS             complement(17034..17597)
                     /locus_tag="IVW29_000956"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7838455.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17848..18117
                     /gene="rpsO"
                     /locus_tag="IVW29_000957"
     CDS             17848..18117
                     /gene="rpsO"
                     /locus_tag="IVW29_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7838456.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18305..20431
                     /gene="pnp"
                     /locus_tag="IVW29_000958"
     CDS             18305..20431
                     /gene="pnp"
                     /locus_tag="IVW29_000958"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7838457.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            20941..21207
                     /locus_tag="IVW29_000959"
     CDS             20941..21207
                     /locus_tag="IVW29_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7838458.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21351..23342
                     /locus_tag="IVW29_000960"
     CDS             21351..23342
                     /locus_tag="IVW29_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7838459.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23696..24997
                     /locus_tag="IVW29_000961"
     CDS             23696..24997
                     /locus_tag="IVW29_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7838460.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(25127..26497)
                     /locus_tag="IVW29_000962"
     CDS             complement(25127..26497)
                     /locus_tag="IVW29_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7838461.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(26679..27002)
                     /locus_tag="IVW29_000963"
     CDS             complement(26679..27002)
                     /locus_tag="IVW29_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7838462.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(27327..27935)
                     /locus_tag="IVW29_000964"
     CDS             complement(27327..27935)
                     /locus_tag="IVW29_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7838463.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            28265..28981
                     /locus_tag="IVW29_000965"
     CDS             28265..28981
                     /locus_tag="IVW29_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7838464.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            28994..29689
                     /locus_tag="IVW29_000966"
     CDS             28994..29689
                     /locus_tag="IVW29_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7838465.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            29773..30402
                     /gene="udk"
                     /locus_tag="IVW29_000967"
     CDS             29773..30402
                     /gene="udk"
                     /locus_tag="IVW29_000967"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7838466.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT         9986 a         5821 c         6283 g         8770 t
ORIGIN      
        1 tttttttatt gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga
       61 ctgcttcgcc cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat
      121 tattggtcaa agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat
      181 ccatttcagc aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga
      241 aaggaacacc ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct
      301 cattagaaag aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta
      361 ctggtgcata gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt
      421 gtgagaaaac tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga
      481 ataatgtttc ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg
      541 gcgttgctgt tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag
      601 caggatcaat cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa
      661 tgatgacagc aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta
      721 gtattcctgg caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa
      781 acatgccagc taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat
      841 tgattccgaa catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg
      901 agatttgttc ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc
      961 cttgggcgat atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa
     1021 tcagctgcat atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat
     1081 acaggaatcc gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta
     1141 cgatatttga gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag
     1201 ctaaaacagt tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga
     1261 tatatttgcg gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa
     1321 taacttgcgc ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa
     1381 tatctgctgc ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg
     1441 taattcctaa aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag
     1501 tgatacctac tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga
     1561 caccattcca aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac
     1621 cttcaagtac atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg
     1681 gtgtaaagat ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg
     1741 cagctgcttt tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt
     1801 tataaaaagt agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca
     1861 tcccttttac gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca
     1921 ggacaaaacg catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga
     1981 tatcatgtaa cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc
     2041 tcttaacttg tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg
     2101 caagcgtttt attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag
     2161 cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga
     2221 tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga
     2281 acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt
     2341 gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac
     2401 ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt
     2461 cgactttcct atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc
     2521 aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat
     2581 cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg
     2641 cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc
     2701 agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc
     2761 ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga
     2821 tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg
     2881 ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc
     2941 ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt
     3001 ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa
     3061 aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa
     3121 cagaacgacc gtttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata
     3181 tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc
     3241 catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg
     3301 gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta
     3361 ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat
     3421 tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat
     3481 aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt
     3541 tattcaagcg attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa
     3601 atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa
     3661 tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt
     3721 atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa
     3781 ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat
     3841 tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa
     3901 agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc
     3961 agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg
     4021 atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt
     4081 gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct
     4141 cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat
     4201 cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga
     4261 gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg
     4321 gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa
     4381 gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg
     4441 ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg
     4501 accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca
     4561 atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct
     4621 agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta
     4681 ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc
     4741 gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct
     4801 catatggaat attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt
     4861 tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg
     4921 cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac
     4981 gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct
     5041 ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga
     5101 ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat
     5161 atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt
     5221 gctactgctt atgcctatga acattttggt tacgactatc atatatgatt tttgctttta
     5281 gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt
     5341 gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga
     5401 agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg
     5461 ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa
     5521 ttgggaagag caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc
     5581 aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa
     5641 atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa
     5701 actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac
     5761 gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga
     5821 tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa
     5881 aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt
     5941 atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg
     6001 aatagcatta gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta
     6061 tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga
     6121 tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat
     6181 cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca
     6241 ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga
     6301 cccatactat accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca
     6361 ttggtggaat atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt
     6421 tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa
     6481 aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca
     6541 gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat
     6601 tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt
     6661 cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat
     6721 cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa
     6781 gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa
     6841 caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa
     6901 tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat
     6961 aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca
     7021 tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa
     7081 aggtcctgct agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag
     7141 ggttgtttgt tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt
     7201 tttcgaaagt tgttaagttt ttttgcgaaa actattttta tatcatatat atgtctaaaa
     7261 tctatattat aaaagaaatg ttgtttgatc agcttctctt cggtattagt tcaaaacaaa
     7321 acggcaaagt atatggagct actttgccgt tttgtttctc taatactcag gtcagtttat
     7381 agacgctcaa taaacggtgt tcaaaagcca gtttctcggt attaagtcaa atttatgcaa
     7441 aaatgtgaga agccattttc aaaaaatatt tcttaatact cgaaactgag cggctttttc
     7501 acgacctctc aataaacagt gttcaagaga cagcctatcg gcaataaggc tgaacgtctc
     7561 tttcacaacc tcttttatca atctgctact tcttctttct tagcgaaact ccaaatcagt
     7621 ccgataacaa aaccgataac tgctggtacg atccacccca tccccaattt gaaaaacggc
     7681 aagtagcttt ccgcaacata taaaagattt tgaacaatag gtgtttgttt gatagtttct
     7741 gggcaagcgt tcaacccatc aaaaatcgaa gcaatcaatg taaaataagt agtcattcga
     7801 taaacagccg tacgctgctt aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt
     7861 gctaatggat agatgaacat caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct
     7921 acgtttgccg ctaaacaagg aaggatacta gcaagtgcaa caaagaatgc gtaatttctt
     7981 tgcggaaaca aatctgtgaa tgtttctgca aaagctgtaa tcaaaccaat accggtcttc
     8041 aagcaagcta atataacgat aaatgctaat aagatactgc cataagtacc taaataatga
     8101 tcagcaatct gagccagcgt gatcccgcca ttttcactta gagcaaagct acctaagctc
     8161 atagcaccta aataagcgag caaagtataa atgattccca tcaatataat gctgatcgca
     8221 ccagatttga tcgtgtcttt agcgatttca gcaggttttg ttacacccat gttttggatt
     8281 gttgtgacaa tgatgatccc aaatgctaaa gcagcaagcg catctaatgt gttatatcct
     8341 tgagtaaacc ctgtgaaaaa agcatgctct tgataatttg gctgaatagg cgcttgattt
     8401 acagaaccta gtggattcaa aaaagcaagg agaagcaaag cacctaataa aaccaaaaat
     8461 gcaggattca ggaactttcc aacataatct aatatctttg tcggttttct tgaaaaccac
     8521 caagccagca caaaaaatac aatactgaaa attgctagat atagcgtctg tctgctagga
     8581 tcgataaatg gtgctaaacc aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa
     8641 aacggtccga taactaagta aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc
     8701 cgttttgcta attcatatac acctgagctt ttcgatacac caatggcaat cacccctaag
     8761 aagggaagac cgataccggt aaccaagaaa cctagattcg cccaaaaggt agcagcgcct
     8821 gcttcttgac ccaaatgaac gggaaagata agatttccag caccaaaaaa tagaccgaaa
     8881 agcatggagc ctataaaaag atagtcttta aatgataatt tttttgtcat acataactcc
     8941 tttacggttt ttttaaaagt aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt
     9001 tcctatataa aacgacggtt atttaataac gtaagtgata aaataaaagc gctgaatcat
     9061 ctgaggagga atcatcgtga caaagtcgag attagaagca tttactgatg gagtggtagc
     9121 cattgttttg agagttcttg tattagagat caagatccct aatacatcta gctttcattc
     9181 tttatgggtc atccgaaata cattattagc ttataccatc agctttatat ttgttgcggt
     9241 gatctgggtg aaccatcatc gtatatttca gatggcagaa cgaattaatt accgagtggt
     9301 ctggtcgaat atctttttgc tcttttggct gactttatgt ccttctgtta ctagttgggt
     9361 cgggcgtaac cctgaagctt tttggcctga gatggcttat gtcttagtat acacgatgtg
     9421 gagtttttct tttggtatct tgatgaggca aatcatgaaa gcaaataaac cagattccca
     9481 tgtcgtgaga gttttgtcaa aggaccgtcg aagtcttatc tccatgatga ttaacttatg
     9541 cttgattgcc ggtgtctttt ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc
     9601 tggtatttgg attttttcct ataaaaaagc agatgcctat tatcagcggt tgtttccggg
     9661 gaaatagaaa agaggctggg acagaagctt ttaactccaa gaaataagcc gaaatttcac
     9721 aaaaatttaa gaagcaattt tcgtaaattc cttcttattt ctcgaagctg accacttctg
     9781 tcacaactac tttcttactt cttacgttca tatttcatat gatagattag taatagaagc
     9841 aagaaccaca caggtgttag taataaagcg attctcgtat cttttcctag gaacaagacg
     9901 acacacacaa aggccatgta aagaaatacc agataattac taaatgggta caacggcatt
     9961 ttaaatgaat gagctttttt gccgaggata cttttacgat atttcatgtg cgtgtagaca
    10021 agcatactcc agataaacaa gaaacaagta gtggcaatac tgcttactag tgtgaatact
    10081 tcactaggca tcacgtaatt caagataaca gaaatgaaaa tgacgatagt agaaaatgtc
    10141 aaagcattcc ctggcacatg atgtgttgtt aactttttga attttttagg ggcactgcct
    10201 tcttgcgcta aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa
    10261 gctgctgcac ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc
    10321 gaaaatactt ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc
    10381 ataatgacaa aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata
    10441 gcttccggca acactttttc aggattttct gtttctccag cgactaatcc aactaattct
    10501 atcccagcaa aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt
    10561 ggaaagaatc cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta
    10621 tagtgggtga ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg
    10681 atcaatgcaa accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa
    10741 agaagaagac ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag
    10801 cgtacataca ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa
    10861 tacgtccagc cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga
    10921 aaagaatggt tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa
    10981 cagatgcatc cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac
    11041 tttccggaac ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca
    11101 tgtctatttt tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa
    11161 ctaaaaaagt ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc
    11221 caaaattttc agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac
    11281 cgcagctctt tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt
    11341 cttttgtacg ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa
    11401 tcacaccttt atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat
    11461 gcgtcacgat aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt
    11521 cgccaaccat ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat
    11581 tcattgataa tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg
    11641 gtttggcttc gatataacga tccataccaa ctttttccag attttcgatt gctacttttt
    11701 tggcagtttc tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga
    11761 cattcatatt gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt
    11821 taggtaaatt atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg
    11881 ttggtttttc cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac
    11941 cgatgatcgt cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat
    12001 tttcaccgaa gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc
    12061 ctccttattc ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac
    12121 catcgatttt cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt
    12181 agatgatccc gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt
    12241 ttccttggaa gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat
    12301 tgataacaaa ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa
    12361 cttttctcat cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt
    12421 catctactgc aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag
    12481 agacaataaa caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac
    12541 cgtagaagat gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga
    12601 tagaaagaag tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag
    12661 gtaccgaacg gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca
    12721 atgcgatgaa caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa
    12781 tcgtagtgaa atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat
    12841 ctttgactgc ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct
    12901 ggtttacttt ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt
    12961 ccgggtttgt ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca
    13021 cactagttgc cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag
    13081 ctaaagctgt tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta
    13141 tctgatcgat cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa
    13201 gatctgctaa actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt
    13261 gtgattcgta ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca
    13321 ttccggcgat gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt
    13381 tcgtttggac gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa
    13441 catcataacc gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg
    13501 tttgttgaga ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc
    13561 tcggtgtttt ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag
    13621 caaatagtac ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata
    13681 tgatacacga gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga
    13741 ccgatttata gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa
    13801 catagcgcaa cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac
    13861 atacactcca agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg
    13921 tttgcttcac tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc
    13981 tcgtattcag ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat
    14041 aactgctgtt ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa
    14101 caatcaaaat gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt
    14161 atgtaataca ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga
    14221 tcatttaagc tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga
    14281 cgccataaat ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc
    14341 atgttcgata tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag
    14401 caaaagccca aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc
    14461 ttaacgctaa ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca
    14521 tatcgattca cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt
    14581 gaacattgtg ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact
    14641 tttcaacaat aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga
    14701 aacaattaac agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt
    14761 tgaccggtgc aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa
    14821 tctaccacgg gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac
    14881 cggataaatt ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca
    14941 ttcatttgga aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca
    15001 tcgacggttt gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt
    15061 atcaatgtca ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg
    15121 acaaacatca agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt
    15181 ttcaagatgg agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca
    15241 ttgttggaac gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt
    15301 cagccaaaat cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta
    15361 ttcaagaaga cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact
    15421 gaattcaaaa ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct
    15481 gagctggttg ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa
    15541 tcagcggaac tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc
    15601 tatatggagc cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt
    15661 tatgcaaatt tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc
    15721 atgttcgctc cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt
    15781 aacagcggtc tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga
    15841 ggagttatcg tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg
    15901 gttgtgacca aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc
    15961 aagacagtag aggaagaact aacagaagag tctcttgaaa cattacgtca taagatcgat
    16021 atgatcgatc gagagttgat tgctttatta gaaaaaagaa tggatacggt aactaaaata
    16081 ggacaagtga aaaaagaaga aaaccaagca gtttatgatg caaaaagaga gcagcaagta
    16141 ttggataaag ttgttagtct gctgaaaaat aagggatata aggaaacaat aacagatacg
    16201 tatgttgatt tgatgaaaca ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg
    16261 tgatcgaagg agagacacgg cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac
    16321 cagtgagtgc tagcaagttt gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg
    16381 atattgcttt actacgagct gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc
    16441 tcgaaacaga agaaaccaat actgggcaca tcagtaaaat cgctgtccag catggaaaag
    16501 atcaaacaga agaagaactg agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa
    16561 ttgtggaaca tcccctttac ggtgagttag caggaacttt gcacatcaaa acgccacaag
    16621 atatcgattc ctttatgaga cgttatcaaa aaagtaatgc tactttgtta tccgaattaa
    16681 caggcggcat ccatctgcat acgatccgct acccagaaaa gcatatgctg aaaaacatca
    16741 aacaaaaact agctgaagca ggaatcctct acgaaggata acatcgtagc tgctactttt
    16801 tatcctagaa actattctat catctctgag ataaacaccc atatggaaga aataagaaaa
    16861 aataagaaaa gatgcgtatc caatacacag aattttttgg aattgcgatt tctagatgtc
    16921 tatctaaatt tcgattagtg gagaaacata aaaaagggaa gtgcagcttt tgagcactcc
    16981 ccttttttat aaagctttct tgtagcatag tatcgaagga acctgccaat tctctactcg
    17041 atcaccaata cgccttcttt tagtgcaaat ggattttgat cgttgatgtg gtcatagaac
    17101 atcacaccgt tgatatggtc gatttcatgt tgaacgacga tcgattcata attcttcaaa
    17161 cgaatcttgt gtttttcacc attcatatca taataagaca cagtgatttt ggcatggcga
    17221 acaacatatc ctggtacttc tcgatcaaca gataaacagc cttcaccttc ccctaaacaa
    17281 gcatcttgaa cagaatggct caaaatttta ggattataca taacagtact tagagaaggt
    17341 ccatctgcct ctgggtcagg acttggtacg tgaacagcaa tgattctttt agaaatatct
    17401 aattgaggag cagctaagcc aacaccgcct cgtaagtgta gttcttcagc tttgattgga
    17461 tcttggctgt tttttaagaa ctcaagcatc tcttttccta aagagatatc ttcttcgctt
    17521 aatggaagag aaacttcttt tgcaacttcg cgtaaggtcg gatttccttc acgaatgata
    17581 tcatccattg taatcatgga atatgtaccc ctttcttaaa agactgtgtt tcaagtctat
    17641 tttcgcatta tagtttacca caaaaatgga aaagtagtag taagattgtt tgaaaactac
    17701 cttttcttaa tgattaacta cttgattttc aacccggttt caggtaaagt atagctgtgt
    17761 gctctaggca catgacctgc cacttggtta agcgaatcac caacgtttta ctcagtcgca
    17821 ggggaataat cgaaagaggt gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc
    17881 atcaacgaat acgctcgcca tgaaggggat actggttctc cagaagtaca aatcgctgta
    17941 ttaactgaag aaatcaacca cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat
    18001 tcataccgcg gcctaatgaa aaaagttggt caccgccgta acttgttagc ttacttacgt
    18061 aaaactgacg ttcaacgtta ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt
    18121 tagtcaaaaa gagtgggatt cacatgaatc tcactttttt tactcgtata tcaattaagt
    18181 aaaattccgt gatcaagcta gatggactcc tactaaccaa atgaaagctg ctcaaaagac
    18241 ggctttcggc agttttcatt tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga
    18301 agttatgtca gaaaaacaag tattcaaaac gacatggggc ggccgtccct tacaaatcga
    18361 agtcggtcag ctagcaaaac aagcaaacgg agccgtttta gtccgttatg gagatacagt
    18421 agtcttaagt gcagctgtgg cctctaaaga agcaaaagat acagactttt tcccattaac
    18481 gatcaattat gaagaaaaaa tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg
    18541 agaaggccgt ccaagtacgg aagcaacatt gactgcccgt ttgatcgacc gtccaattcg
    18601 cccgatgttt gcagaaggct tccgtaatga agtccaagta acgaacatcg tgatgagtgt
    18661 agaaacagac tgctcgccag ctatggcagc aatgctaggt tcttctttag cactatcaat
    18721 ctcagatatc ccatttgacg gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga
    18781 gtacgtcttg aatccaacag tagaacaagc agaacaaaca gatatcgaat tgactgttgc
    18841 cggaacgaaa caagccatca atatggttga aagcggcgcg aaagaagttt cagaagaaga
    18901 catgcttggt gcattgctgt ttggttttga cgctatcaaa gagttagttg ctttccaaga
    18961 agaaatcgtt caagcagtag ggaaagaaaa aatggaagtg actttactgc aagttgatga
    19021 agtattgaaa aaagaaatct ttgatgcttc ttacgctaca atgaaagcag cagtaatgac
    19081 tgaagaaaaa cttgctcgcg aagataatat cgagcaggtg aagatcgata tccgcgaagc
    19141 ttatgctgag aaatttgcag gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca
    19201 aatcacagaa gatcttgaaa aagatgtcgt acgtgaattg atcaccatcg ataagatccg
    19261 tcctgacggg cgtaaattgg atgaaatccg tccattgtca tcagaggtca gcttattgcc
    19321 acgtgtacat ggatcaggtt tgttcacacg aggacaaaca caagctttat cagcttgtac
    19381 actcgcacct ctaggagagc accaaatcat tgatggttta ggggtggaag tcagcaaacg
    19441 attcatccac cactacaatt tccctcaatt ttctgttggt tctacaggac gtgcaggctc
    19501 tccagggcgt cgggaaatcg gacatggcgc attaggtgaa cgtgcgttag cacaagtaat
    19561 cccttcagaa gaagaatttc cttatacgat ccgtttagta gcagaagtat tggaatcaaa
    19621 tggttcttct tcccaagcaa gtatctgtgc tggcacatta gcattgatgg atgcaggtgt
    19681 accaatcaaa gcgccagtcg ccggtattgc gatgggactt gtttctgatg gtgaaaacta
    19741 tacgatccta actgatatcc aagggctgga agatcatctt ggcgatatgg actttaaagt
    19801 tgccggtaca aaagatggta tcactgcttt acagatggat atcaagattc aaggaatcac
    19861 agaacaaatc ctaactgaag cattaactca agcaaaacaa gcacgtatgg aaatcttaga
    19921 agaattgact tcaacgattg ctgctccacg tgaagaatta agccaatatg caccaaaaat
    19981 cgaaatgatc cagatcgaac ctgctaaaat caaagatgtt atcggtaaag gcggcgatac
    20041 gatcaacgga atcatcgatg aaactggtgt gaaaatcgat atcgatcaag atggaaaagt
    20101 aagtattgct tcagctgatg cagaaatgat caaaaaagct atcaagatca tcgaagattt
    20161 gaccaaagaa gtcaaagttg gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt
    20221 cggtgcattc gtcaacttga tcaaaggaaa agatggactt gtccatattt cccaattagc
    20281 aaacgatcgt gtcaataaag tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa
    20341 agtaactgaa atcgataaac aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga
    20401 agatggttca acgaaaaatg aagaaaaata atttaattca taggaataat gtacttgtga
    20461 tcgtgaaggt gacaggggga caataattat tcgaaaaaag gggactgtga caaaagccgt
    20521 gtcacagtcc ttttttttcg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt
    20581 ccagccaagt taaaaacatt aggagctgtt tttatttggc tgttcttatt acttgatgca
    20641 gaacagcttt tttacaacct ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta
    20701 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcaggt
    20761 caaaccgctg atttcacaac ctctgattca cggtgttcca gaagcagctt ctacggaaat
    20821 aagctgaaca cttttgttac aacttctttt tttctggagt ttcttggcgc atatttttgg
    20881 agtccttctg aataaagcgt aaaataaaag tgagaaatat ggataaagga gggaaaggag
    20941 atgctgaatt ttttatggtc gttatttatc ggcggtatat taggtttgat agctggcgca
    21001 attttaggaa aggaagtccc gggaggcgta tctggaaata tcattatcgg ttttttaggt
    21061 agttggctcg gcgaattttt actaggacca ctgggaccgg aactcggcgg tttttatttg
    21121 ataccagcat tattgggtgc aattttttgc cttgctcttt attcatttgt ggcaagacgt
    21181 atcagagaac gtccaaagaa agaataattt tattcaaata agttgacact tcagtgtgtt
    21241 acttggataa ttagaagcaa tttaggaaag cgggtgtcat ggataagaat atcatctatc
    21301 attcaagctt acctaaagga tatccatata acaagaaatg agggaaaaac gtggcaacaa
    21361 cgcacaatac caaaaagata tcaatgactg gcctgcttgt agcgatggga gtcgtctacg
    21421 gagacatcgg gacaagtccg ctttacgtta tgaaatcgat tgttgaagat aatggcggat
    21481 tgtcaggaat caatcctgat tttgttatcg gatcagtctc attgattttt tggacattga
    21541 ctctactgac aacaatcaaa tatgtcgtga ttgcattgaa tgcagataac catggtgaag
    21601 gtggaatttt ctcactctac acacttgtcc gaaaaggtgg gaaatatttg atcattcctg
    21661 caatgatcgg aggagctgcc ttactagcag atggggtatt gacccctgct gtgacggtaa
    21721 cgactgcgat tgaaggcttg cgaggaattc cgcagttttt tgaccggttc ggtaataacc
    21781 agaacgtgat cgtcattatc actttagtta ttatttttgt tttattcatg gtccaacgct
    21841 ttggtacaga tattgtcgga aaagcattcg gaccaatcat gttcgcttgg tttacttttt
    21901 taggcgtaat gggattaatg aacttcagtc aagactggac agttatccgt gcactgaatc
    21961 cttattatgc tatccagctg ctttttagtg cagataacaa attaggcttg ttcattcttg
    22021 gaaatatttt cctggctacg acaggtgcag aggcgcttta ttccgatctt ggacatgtcg
    22081 gcaaacgaaa tattcgtctg agctggcctt acgtcaaaat ttgtttggtt atcaattatt
    22141 ttgggcaggc tgcgtggttg ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga
    22201 acctgaatcc attcttccaa atgatgcctc aaagttggat cgtgatcggc gttgcttttg
    22261 caacagtcgc tgcagtcatt gcttcgcaag cattgattac aggttcgttt acgctagttt
    22321 cagaagcaat caagttgaaa ctgctgcctc ggttaaagat catgtatcct gggaacagta
    22381 tcgggcaaat gtatatccca gcagtcaatc tgattttatg gatcgcctgt tctctagtgg
    22441 ttgtcacatt ccgaacgtct catcatatgg aagctgctta cggtctatcg atcactgtga
    22501 cgatgttgat gacaacagca ctgctttact tttatcttat ccaaaatggg cattctcgtt
    22561 ggcttgcata tttagttacc tttttcttcg gtgcgataga agtcgttttc tttatctcaa
    22621 gtgttgtcaa attcttccga ggcggttttg tagccgttct gattggtttg atcatcttag
    22681 tcgtgatgtt tatttgggaa caagggaaca ttattcgcga atcgtcaacc gaggatgtgt
    22741 ctttgagaga ttatatcccg cagcttagag aattacgtga tgatacttct ttaccaatct
    22801 atcagacaaa tgtggtcttc ttggttcctg atatggctga agataaagtt ggccgacagt
    22861 tcatctattc gattttagat aaacggccta aaagagcacg agtctattgg tttgttcatg
    22921 tggaagtgac ggatgagcca tatacgaaag aatatcaagt agatatgatg gacactgatt
    22981 ttattgtcca agtgaatctg ttcttaggtt tcagggtcca acaggaaatc aacgtatatg
    23041 ttcgccaagt catccatgat ttgatgaaac aaggccgttt gccaaaacag ccacaaagat
    23101 actctctgac accagggcga gaagttggcg atttccaatt tgtcctgatt gatgaagtag
    23161 tttccaatgt gacaactcta ggaaaatggc aacgtcaaat catgcaggca aaactggcaa
    23221 tcaaaaaaat cgctacctcg ccagaaacat ggtttgggct agagtacagc gaagtaaaac
    23281 atgaaactgt tccgcttatc attggaaata ctcggaaaac atggttgaaa gagcggttgt
    23341 gaaagtagcg ctttgacctg aactaatgtc gaatgtcagc tacttgaaca ccgtttatca
    23401 gaggtcatga aaaagctgta ctgcaacaag cagtaagaac gagcagataa aaacagcttc
    23461 tcatgttttt catctgtttg ggcttaatgc cgtagatgca agcttttgaa taccgttttt
    23521 atcagaggtc atgaaaaagc tgtttagcaa caagtaacgc aatgattttg aagtgataag
    23581 gagatgtggc tgaaaaatgc cgcatctcct tttttattcc taaatatgaa tacgcattat
    23641 tttcaatctt tttgtagaaa aagggtacaa tagaaaaaga gaaaggcggg agaatatggt
    23701 tatcaatttc attattttat ttttgatgat tgcaattact tcattttttg tagcaagtga
    23761 atttgcttta gtgaaaatca gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa
    23821 gaacgccaag ctagctcttc atgttacaca ccatttagat aactatttat cagctagtca
    23881 attaggaatt acgctaaccg gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc
    23941 tttactagaa ccagtgatcg gtaaactacc ttttagttcg gctattagca gtacaatctc
    24001 tgtggcgctg ggatttattt tagttacgta tatcgatgtg gttattggcg aactgcttcc
    24061 taagagctat agtatcgtca atacggagaa ggtcgtcttg ttcgtagtta aaccacttca
    24121 ttatttttat aaagtaatgt ttccttttat ttgggtattg aatcattcag ctgcagggct
    24181 agggaagcta ttaggtgtgc gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga
    24241 gctgacatta gtcgcattga attcttatga aaaaggagag ttgaccaaag aagaatatta
    24301 ttatttggaa aacgtgtttg aatttgatga taccttggca aaagatatac aagtagaccg
    24361 tacgtccatg caagtattcg aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat
    24421 tgaacaagga catactcgct atccggttat cttagaatca aaagacaatg ttttaggtta
    24481 tgtcaccttg ccagatttga tcaagcaatc ttataaagat gaccagctga cagtagaaca
    24541 gttgattgaa gagccgatcg taacaactga aacgatccct ataaaaaaat tattgacgat
    24601 catgcgaaaa aagggtaagc atattgctat cttaaaagat gaatacggag ggacaagcgg
    24661 cttagtgacg attgaagata ttttagaaga aatcgtcggg gaaatccgag atgagacaga
    24721 tttggacgaa gcattgatag cggaacagtc agatggctct tatatcatct ccggcaaact
    24781 aacattagat gatttccagc gttattttca tgtagagatt ccagagtttg aagaaacgaa
    24841 ttttactaca cttgcgggct ttgcttctag ccgatataaa gaaataaaag caggaacaat
    24901 cattgagatt gcatcattcc gtttcacggt attagaatac cagcatgcac atatcgatta
    24961 tttcaaagta gagtccacgg aaagaaaaac agaataaaag aaaaaaggct gtgacatttt
    25021 ttgtcacagc ctttttgtta tctaaaaaac ctgttttcgg taaacgaaga agatattttc
    25081 aataaaagtt ttcattttta gccgcaacga tttcttttaa tcaaggctat cttgcttctg
    25141 catgttctaa tgtgaaatgt tcctcttcac ccatgtgttt caattgtttc atcgaacgca
    25201 tataagagta aagcaacacg gcaacagaac cgatccaagc taaaggagaa gctgctgctg
    25261 ctcctgggta tccccacatt cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt
    25321 ccatcatacc ggcgatcgta gggatcttgc tttgtccaag cccttgaagt gtgtagcgta
    25381 aaatgaacag gactgctaag atccagtaaa gagacccatt gatattgaag taggtttgtg
    25441 ctaattcaaa tacacgtgtt tctgatgggt taacaaacaa ttgaacaaag aaatgaccga
    25501 agaaaatgac gatagcacct gctaagagac taaatccgac actcatcagt aaagtttgac
    25561 gtaccccttg caagattcgg ccatattctt tcgcaccata gttttgtgca gtgaaagttg
    25621 ccattgcaat accaaatgac atcatcggct gatttgcaaa ttggtcgata cgaccagcag
    25681 cggcttgagc agctacgaca tctgtcccta gactatttag tgctgcttgt aacacgatag
    25741 caccaacagc gatgatcgaa gattgaaacg ccattggcaa agcagcattt aggtgtacag
    25801 caaactcttc tttttcaaat ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt
    25861 tcttgatata cactagacac agtacgctgg aagaaacttg tgcgattact gtagcaatag
    25921 ctgcaccttc cacacccatg ccaaaataga taataaagat caaatcaagt acgacgttga
    25981 tgatgaccgc aatgatcaga aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga
    26041 cattagaaag caaattgaaa ctgactgatg caaagatccc taataaaata attgaaataa
    26101 atgtttgtgc ttgatcaagt atttcaggag gagtttgcat caattgaagc atcggacgaa
    26161 taaacaggag acttaggaca gttaaaatca cagtgacgac aagactaatg acaacactag
    26221 cagcaaaact tttcttcaga ccccgataat cttttgcacc gtaacgttgt gcggtaataa
    26281 tggctaaacc agctgttaaa ccttgggcaa agccaatgat caaaaatgtc aggcttccag
    26341 ttgcaccaac cgctgccagc gcatttttac ctagcgtctg acccacgatg atcatatcga
    26401 tcatattgta gaactgctga aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa
    26461 tcagtttagc ggggcttcct tttgttaaat cgcgcatctt ttaaactcct tatatcttta
    26521 aaatttagtt agtagtatat agcgtttatc ctgagaatac caattagaaa tcaatgataa
    26581 tttatgaaac tccataaaaa gaagccaaga attgagtcaa agaatcaaca acttggcctg
    26641 gtctgtaaaa taagggacat ccacgtttat ctatcacctt aatattgtct aatctcactt
    26701 ggtaaaagga tcgtttctct cgtaccgtca tcataggcgt atacttgagc aggaaattgg
    26761 ttcgaatagg caaacggcaa atcaatatcc atttccacat tgcttcctgc ttgagaaaaa
    26821 tgcatcgtca cttttccacg attttcgatc aaatcaaagg ttaatatatt ttctaatgga
    26881 atgactccct taagatctag gtcaataata aaccatatac tgtcaatcaa ttcatctggc
    26941 aaacttgata cgacaccaac tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac
    27001 atctgtttaa cctccttcga atcaacgaat gttttatttt tataattaat cctaggataa
    27061 actagaaata cttacttcaa cgagggttca ttctaataaa gaatcaggaa aaatgcaaca
    27121 ataattgaat actttttttc aaagtataaa tttttataaa ggtacgataa aagtatgcga
    27181 ggaaaagctt ggtaaattag gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc
    27241 catatgaaaa taaacgatta gaaataaaaa aggcgtgtta aaagctgctg acccgccgct
    27301 tctaacacca cctgattgtt tcagtattat tcttctgttg aaccgatcac ttctggttcg
    27361 ccaccttcac ctgtgctttc ttcttcttca ctttgagcga ctacagcagc gatttgctct
    27421 tcactgtcgg tcacgaattt gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa
    27481 tcacctaatt ccaagtttgt cacgtcaact tcaattcgtt caggcaattt gtctggtgta
    27541 gcagatacga ctactgtata caagttttga gctaacactc caccagattt agcacccact
    27601 gattcgccaa caagaacgac ttctgtttcg acttctgttt cttccgacat gtttacagat
    27661 agaaattctg catgttcgaa atgattggta aaagtgttcg tttggatctt gtgaaccaat
    27721 gtattgactt tttgtccatc gatgtccatc gtgatgactg tgttcgctcc atgttcacgc
    27781 aacaatttgc ttagtgtcaa accatcgaat gagatagggg tactttctac tttataccca
    27841 taaacaacag ctggaacttt cccttcgtga cgaagtttgt ttcttaatga acggggacgt
    27901 acttctcttt tacttacttc taatgatact gacataacca aaattcctcc ttaaaatgtg
    27961 actgtctgct ttggtaacgg acagatttta tttaagtgaa ttctaaaggc taatctcgtg
    28021 cacatcatcg aaggcagggc gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc
    28081 ttagaaagtc aggattcaac ttttctccac caggtcatta cgctgtgtat gagaaatcca
    28141 attcacttta ctatttaacc gtaacgtgaa ctgttaatta agtcaaggaa taactatgat
    28201 gatgtatttg caagcaagaa tattcaaggt ataattaatt agtagtaata agaaaggaaa
    28261 cagcatgcgt ttagataaat taatagaaga aaaattagaa acgacacgca aggaaatgaa
    28321 gcggctgttt gctttgaagc aagtgatgat agatggcaca gtcgaatttc gacaaaatcg
    28381 aaatgtagat agtttactgc atcaaatcga agtagcaggt gaacacttag aaacaaatga
    28441 gtcctattat cttctcaata aacctgaaaa agctgttaca gctgtatcag atccagaaaa
    28501 gacgacagta atcgatctga ttgcaagtgc cgatcgtttc caaccattat atccagtggg
    28561 aagactagat agagatacaa caggactatt gctgatcact tcgaatggac aattgggata
    28621 tgaaatgcta cttccagaaa aaaaggtttc aaagacttat caagtagttg tgaacgaaag
    28681 ggtgacaccg gcagacgtaa cagcatttga aaatggaatc gtattcgctg atgggtatca
    28741 atgtaagccg gctgatttaa aaatactcaa agcagacaag caccaaagta cggttttatt
    28801 gacgatccaa gaaggaaaat tccatcaagt caaaaagatg ttcttagccc aaggaaagaa
    28861 agtgaccgca ttgaaacggc taagcatggg accattggaa ttagatgaac agctaggagt
    28921 aggcgagtat cgatcgttga cattagcaga gatgaaaaag ttaagtattt attttaaata
    28981 ggaggtcaaa aagatgaaat ataaaacttt attgtttgat gtagatgata cattgttaga
    29041 ttttcaatta actgaagcca atgctttaag tgctttgttt gcagaacaag gaatcacatt
    29101 gacaccagaa atcgaaacca gctataaggc tttgaatcat catttatggc aggagtttga
    29161 aaaaggcaga ctatcacgag aagaaataat aggcagtcgt tttgggttac ttttccagca
    29221 atttgggaaa acagtagata gtgatcaaat ggaaaaacgc taccgccact acttgaacca
    29281 aggtcatgat cttgttccag gaagtctaga aatcctgaaa aaggtccatc cgcatgctga
    29341 tctttatatt gtgacgaacg gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg
    29401 aatgcttgat tacttcaagg atatctttgt ctcagaagct gtcggagcgc aaaagccgat
    29461 gaaagaattt tttgatcatg tcttttccca cattcctaat tttgataaaa agcaaactgt
    29521 gatcattggt gattctttga cttctgatat caaaggagga aatatggcag ggatcgatac
    29581 gatttggttc aatagaaata aattaccaga aatcgaagaa atccagccta cttatcgaat
    29641 cgattcatta gaggaacttt attcattatt ggaaattgaa aaggattaat tttcagaaaa
    29701 atatagcatt ttcgtgtttt ttcgctataa tagattagaa tgaaaaaacg gatacgaaag
    29761 aggttttagt caatgacaaa aagcaagcca attatcattg gtgtgacggg tggatccgga
    29821 agcggaaaga caagtgtcag ccgtgcgatt ttcaacaact tcccggacca ttccatcatg
    29881 atgcttgaac aagattctta ttacaaggat cagagccatt tgagttttga agaacgattg
    29941 aacacaaatt acgatcaccc atttgccttt gataatgatc tgctgattca gcatgtaggc
    30001 gacttactga actataaagc cattgaaaaa cccgtgtatg actatgtagc gcatacaaga
    30061 agtcaggcaa cgatcattca ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt
    30121 ttagaagacg aacgtctgcg tgacttgatg gatatcaaag tatacgtaga tacagatgat
    30181 gacattcgta tcattcgtcg tatcaaacgg gatatggaag aacggggacg cacattggac
    30241 tccgtgatcg aacaatattt gacagttgtt aaaccgatgt atcatcaatt tatcgaacca
    30301 acgaagcgct atgcggatat cattgttcca gaaggcggag aaaaccacgt tgctattgat
    30361 ttgattacaa caaaagtagc tagtttctta aaccataaat agtgcgcagc gaaacagttg
    30421 aacggactgc gaccgtttga aaagcaagga ttctgctttt tgaacagtca ccagccaact
    30481 gtttttttat ttataacaag aggttgtgaa aacagcatct tgacctaagt attaaaaaga
    30541 agaaacggaa aatagctgtt tatattttac cgttttttga gctaataccg aaggtcagct
    30601 gattgagcac cgtgaagtag aggttgtaaa aaagctgtct ttgcatcaag cgataagagc
    30661 atccaaataa aacagctcct cacgtttttc gttgggttgg gcttattatc gtagatgcaa
    30721 gcttttgaac accgtttaac gagaggttgt aacagaagtg gtcagatgca ataaataaga
    30781 agaaacttac gaaaattatt tctcgaattt gtgtgagatt tcgacttatt tttttgaaga
    30841 agctcttttg tcgcaccccc
//
LOCUS       DACCVU010000025        30818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCVU010000025 DACCVU010000000
VERSION     DACCVU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.025"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            623..1951
                     /locus_tag="IVW29_000968"
     CDS             623..1951
                     /locus_tag="IVW29_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7838467.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2153..2986
                     /locus_tag="IVW29_000969"
     CDS             2153..2986
                     /locus_tag="IVW29_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7838468.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3002..3739
                     /locus_tag="IVW29_000970"
     CDS             3002..3739
                     /locus_tag="IVW29_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7838469.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3894..4862)
                     /locus_tag="IVW29_000971"
     CDS             complement(3894..4862)
                     /locus_tag="IVW29_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7838470.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5094..5927
                     /locus_tag="IVW29_000972"
     CDS             5094..5927
                     /locus_tag="IVW29_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7838471.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5961..6800
                     /locus_tag="IVW29_000973"
     CDS             5961..6800
                     /locus_tag="IVW29_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7838472.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIINELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6826..7386
                     /locus_tag="IVW29_000974"
     CDS             6826..7386
                     /locus_tag="IVW29_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7838473.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7585..9306
                     /locus_tag="IVW29_000975"
     CDS             7585..9306
                     /locus_tag="IVW29_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7838474.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRTLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9471..10613
                     /locus_tag="IVW29_000976"
     CDS             9471..10613
                     /locus_tag="IVW29_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7838475.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10629..11840
                     /gene="thiI"
                     /locus_tag="IVW29_000977"
     CDS             10629..11840
                     /gene="thiI"
                     /locus_tag="IVW29_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7838476.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12413..13060
                     /locus_tag="IVW29_000978"
     CDS             12413..13060
                     /locus_tag="IVW29_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7838477.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13453..16098
                     /locus_tag="IVW29_000979"
     CDS             13453..16098
                     /locus_tag="IVW29_000979"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7838478.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16408..17721
                     /locus_tag="IVW29_000980"
     CDS             16408..17721
                     /locus_tag="IVW29_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7838479.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17711..18364
                     /locus_tag="IVW29_000981"
     CDS             17711..18364
                     /locus_tag="IVW29_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7838480.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18419..19111
                     /locus_tag="IVW29_000982"
     CDS             18419..19111
                     /locus_tag="IVW29_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7838481.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19261..19461
                     /locus_tag="IVW29_000983"
     CDS             19261..19461
                     /locus_tag="IVW29_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7838482.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19715..21394)
                     /locus_tag="IVW29_000984"
     CDS             complement(19715..21394)
                     /locus_tag="IVW29_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7838483.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21396..21608)
                     /locus_tag="IVW29_000985"
     CDS             complement(21396..21608)
                     /locus_tag="IVW29_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7838484.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22001..23731
                     /gene="efrA"
                     /locus_tag="IVW29_000986"
     CDS             22001..23731
                     /gene="efrA"
                     /locus_tag="IVW29_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7838485.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23728..25488
                     /gene="efrB"
                     /locus_tag="IVW29_000987"
     CDS             23728..25488
                     /gene="efrB"
                     /locus_tag="IVW29_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7838486.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25583..25780
                     /locus_tag="IVW29_000988"
     CDS             25583..25780
                     /locus_tag="IVW29_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838487.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25933..26418
                     /locus_tag="IVW29_000989"
     CDS             25933..26418
                     /locus_tag="IVW29_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7838488.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26522..27115)
                     /gene="clpP"
                     /locus_tag="IVW29_000990"
     CDS             complement(26522..27115)
                     /gene="clpP"
                     /locus_tag="IVW29_000990"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7838489.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27295..27822
                     /locus_tag="IVW29_000991"
     CDS             27295..27822
                     /locus_tag="IVW29_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7838490.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27913..28650
                     /locus_tag="IVW29_000992"
     CDS             27913..28650
                     /locus_tag="IVW29_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7838491.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28654..29571
                     /gene="miaA"
                     /locus_tag="IVW29_000993"
     CDS             28654..29571
                     /gene="miaA"
                     /locus_tag="IVW29_000993"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7838492.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29573..30802
                     /gene="hflX"
                     /locus_tag="IVW29_000994"
     CDS             29573..30802
                     /gene="hflX"
                     /locus_tag="IVW29_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7838493.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10177 a         5273 c         6575 g         8793 t
ORIGIN      
        1 cgtaggttcg agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa
       61 tcacttgggt ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg
      121 tatgttgtaa tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat
      181 catcgctaaa tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa
      241 gaaaagggat gacattccca aagttatctg tattcataag tatgccacca cccacaaaca
      301 aaaagccgcc agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt
      361 tccttctttc aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct
      421 tctttattat acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact
      481 attgtatacc gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa
      541 aatttcatca tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac
      601 ttggatttgg aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa
      661 caaacagttt caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg
      721 aattactgct tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat
      781 agatttggct acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc
      841 tgcactgaac acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt
      901 atcacaaaat tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa
      961 catcattgca acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac
     1021 tcctcaacaa gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt
     1081 tgtgcctaaa tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa
     1141 ctacttgaac aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg
     1201 agaaacatta gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt
     1261 cttagctatc tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga
     1321 aaagaactat catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg
     1381 tgtaaaatta gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa
     1441 tcgttcttat acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct
     1501 gaatgttgat atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc
     1561 tgtttggttt ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt
     1621 agatgcttat tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg
     1681 tttagatttt ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt
     1741 agacgggcaa tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa
     1801 tggcttcttt gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag
     1861 aaaagattta ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc
     1921 tccgtgggat ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta
     1981 aagataaaaa gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt
     2041 ttatggtccc tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc
     2101 attaaaaata tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat
     2161 ccaaactgtt ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga
     2221 tgttaatcag acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc
     2281 gaatgtaaaa aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt
     2341 cgtacgtgta ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat
     2401 ggaatggatc gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct
     2461 ccatcagcaa acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt
     2521 tcagaaaaat tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc
     2581 ccagatttct ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct
     2641 gcgatttaaa gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt
     2701 gcacggagac ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt
     2761 cgacccagct gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg
     2821 tggtttccga ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg
     2881 gaaagaccga ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg
     2941 agaaagttac ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt
     3001 tttggcaaaa aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca
     3061 aacgatgaca tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga
     3121 ccagttggga agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa
     3181 ttattccatg gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca
     3241 agccatggag tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg
     3301 gaaagaatta gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat
     3361 gagcagtgtt aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa
     3421 gcgtcaggaa aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt
     3481 ttatgatatc gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga
     3541 cgtattagga tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga
     3601 taaagaagta gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa
     3661 tggtgtcaaa acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat
     3721 tatatggaaa aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag
     3781 aagaaattta cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga
     3841 agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt
     3901 tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt
     3961 aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct
     4021 tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca
     4081 agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg
     4141 agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct
     4201 tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca
     4261 gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta
     4321 gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg
     4381 gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta
     4441 tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca
     4501 cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca
     4561 atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc
     4621 actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga
     4681 gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc
     4741 ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc
     4801 gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc
     4861 attttacaga cttccttacg aataattttg catagccgtt aaatcttatt gtatactata
     4921 taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg
     4981 accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg
     5041 agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca
     5101 gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc
     5161 tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc
     5221 cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtag
     5281 tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag
     5341 aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt
     5401 tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact
     5461 tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt
     5521 cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt
     5581 ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg
     5641 tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag
     5701 caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc
     5761 aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt
     5821 ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa
     5881 atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat
     5941 tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg
     6001 tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa
     6061 acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcatta
     6121 atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc
     6181 gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag
     6241 atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga
     6301 tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc
     6361 cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt
     6421 atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt
     6481 cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt
     6541 atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa
     6601 atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg
     6661 agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac
     6721 atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac
     6781 acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca
     6841 ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc
     6901 acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt
     6961 gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg
     7021 cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat
     7081 ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt
     7141 tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg
     7201 aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct
     7261 gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag
     7321 attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa
     7381 gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc
     7441 gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca
     7501 cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc
     7561 atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca
     7621 atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa
     7681 gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa
     7741 gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa
     7801 tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt
     7861 ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt
     7921 gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta
     7981 aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc
     8041 tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt
     8101 gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac
     8161 acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat
     8221 gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc
     8281 cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta
     8341 ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca
     8401 ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct
     8461 gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta
     8521 aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa
     8581 ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc
     8641 cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt
     8701 ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc
     8761 ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa
     8821 ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc
     8881 actttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa
     8941 ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga
     9001 gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta
     9061 gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat
     9121 gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat
     9181 ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag
     9241 cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta
     9301 gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg
     9361 tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac
     9421 cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt
     9481 ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca
     9541 gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt
     9601 tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct
     9661 attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa
     9721 aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga
     9781 cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg
     9841 gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg
     9901 ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt
     9961 tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac
    10021 cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc
    10081 atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac
    10141 tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg
    10201 tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga
    10261 cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt
    10321 tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg
    10381 gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct
    10441 gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc
    10501 tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc
    10561 aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga
    10621 gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa
    10681 agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact
    10741 ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag
    10801 acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa
    10861 gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag
    10921 aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg
    10981 aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc
    11041 atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg
    11101 gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg
    11161 gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca
    11221 tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac
    11281 aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc
    11341 attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag
    11401 gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta
    11461 ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt
    11521 tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga
    11581 caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta
    11641 tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc
    11701 aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag
    11761 cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag
    11821 aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa
    11881 acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc
    11941 tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa
    12001 aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc
    12061 ccatgaaata gcgaggttgt gacagaagtg ggcagcttca agaaataaga aggaatttac
    12121 gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg
    12181 gaacaccgtg tatcagaggt tgcgaaaaag ctgccctgca tcaagtaata agaacagcca
    12241 aacaaaaata gttcctcata ttttacgttt ggttgagctt attaccgcag atgcaagctt
    12301 ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc ttgctcccgt
    12361 gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg ttatgcgaga
    12421 aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt tacgctactt
    12481 gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca gcgaaatcac
    12541 ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc taggcagaag
    12601 cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc ttaatactaa
    12661 agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat tgaaacacaa
    12721 caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg atccagcact
    12781 gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg cggagatagc
    12841 caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt cacaatctgc
    12901 tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc ctgacagagt
    12961 aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt tgcagacgct
    13021 tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga gtaggcttat
    13081 tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa atgaagtggt
    13141 tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag cataacgctg
    13201 aacaagagga gtaactggca cattcattct tagagagaga actatctgct gaaaggttct
    13261 caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta gagatagacg
    13321 agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa tttaggtggt
    13381 accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt tagtttaaag
    13441 gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc gcaagaagtt
    13501 gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag cggagataaa
    13561 aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa acttcattta
    13621 gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag aatgcaagga
    13681 tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca agcaaaagta
    13741 gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga aaagttcatc
    13801 gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca gtgggctaaa
    13861 atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg attatctgaa
    13921 gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg tggtgaatat
    13981 atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt tattcataaa
    14041 gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc aggcgtagta
    14101 gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc tgttcatcca
    14161 gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt agtcaataaa
    14221 caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg tgtagtaaaa
    14281 atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga tcttccacgc
    14341 gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata tgctggaatg
    14401 gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg tcgattgatt
    14461 aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa
    14521 cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat
    14581 aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca aacgttttta
    14641 cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg gggacatcaa
    14701 attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga agcaccagcg
    14761 gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag ttcagctttg
    14821 tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa gcgatacttc
    14881 cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag cagaatgatt
    14941 ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat tcatggattg
    15001 attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat cgatccgatg
    15061 gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa tggttctgca
    15121 ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa ctttatcaac
    15181 aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac cgtagaagag
    15241 atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg tttgaatgaa
    15301 acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc aggtcgacaa
    15361 ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag taaagaagta
    15421 ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt ccatacactt
    15481 gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga aatatgggaa
    15541 catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt acgtccagag
    15601 tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat tcgagccgtt
    15661 cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt gctaattaaa
    15721 acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat cgaacgcttc
    15781 tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct agcgatgtca
    15841 gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa tattgaagaa
    15901 gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa
    15961 ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt agaggcagag
    16021 cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg aatcgaaagt
    16081 ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc tattactaaa
    16141 agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta gcttcgattc
    16201 ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct tattatgtag
    16261 gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata aaatcgttcc
    16321 tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc tatttgttat
    16381 aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga atggatccat
    16441 agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt attagaagtg
    16501 gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt
    16561 tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt aggtacattt
    16621 acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca
    16681 gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa
    16741 tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc atttgattat
    16801 tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg cttattggat
    16861 tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact tgatcatacc
    16921 gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat catcaaacaa
    16981 ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat ccaacaaaca
    17041 gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat aaactatctg
    17101 catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg aaagatccct
    17161 caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt agccatcgaa
    17221 ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga tgttttgaag
    17281 ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga gccattagtt
    17341 gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa
    17401 gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa aaatgttaga
    17461 gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac gacctttgaa
    17521 catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag agtatcaatc
    17581 gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa tgaagacgta
    17641 ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt aatacaatta
    17701 ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc tgttcgacac
    17761 agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt ttccttatga
    17821 taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg cgaattatca
    17881 tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg attcatacca
    17941 ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg tgttggaact
    18001 cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta accaacgagc
    18061 agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga cgattgtttc
    18121 tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag ctaatcagaa
    18181 gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg gtattttagc
    18241 agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac catcccaaga
    18301 attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg cattgttaaa
    18361 ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag atgttttttt
    18421 gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac gtatggaaat
    18481 ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc gtaccggtca
    18541 acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg gaggactggc
    18601 ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag gacgcgtgaa
    18661 agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg atcatgaaag
    18721 agcggctcca ccggttcgta ccagctacga attggcccag caattgattc tggaaatgaa
    18781 agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc aagtgatctt
    18841 aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta gagaaatttt
    18901 ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc atccttctgg
    18961 taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga
    19021 aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt
    19081 gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa
    19141 gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct
    19201 atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt
    19261 atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt
    19321 gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca
    19381 ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct
    19441 gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt
    19501 tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc
    19561 aggtattttt ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt
    19621 atcaaggaat aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata
    19681 ttcaggacaa ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca
    19741 ttggcaaaat cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag
    19801 tcatcgcttc tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag
    19861 cgtggaataa gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa
    19921 atccacgaga gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg
    19981 cgacagctaa tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac
    20041 caatatcacc gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag
    20101 gacgcgcaga atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt
    20161 ctgcaggaac acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc
    20221 cgtggacagg aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt
    20281 gtccaccgtg accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag
    20341 cttcagacag caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac
    20401 ttgaaaagat cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga
    20461 tgcggctcaa cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat
    20521 tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat
    20581 atcctaaacg ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt
    20641 tacgaccagt tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa
    20701 agctggcaaa aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa
    20761 tcgttttttc tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc
    20821 acaagactcc ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag
    20881 gagtgaaatc gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca
    20941 cgatccctaa tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc
    21001 taaaacggat gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat
    21061 gctcatctaa tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat
    21121 ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca
    21181 ccaaggcttt tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt
    21241 caattccaag taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat
    21301 cttggaattg taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga
    21361 cgcctgtctc gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca
    21421 gcattttctt tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg
    21481 tatggggtat tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa
    21541 agagattttg tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta
    21601 tagatcattg ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt
    21661 tagacaacac aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac
    21721 tggattcgag tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac
    21781 cgtaccttct gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa
    21841 taagtataga aatcaactag attgaaagaa aaaatcaatg tttatcatcc cgctataatc
    21901 ataatgactt ttttatgaga gaagtgttct attaataggc tcgttgtgct atcattttta
    21961 gtaatgaaaa tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa tgtggcatta
    22021 taccatgcgt tataagaaat tcttgttttt caatttcata tgcgtatttg gttttatttt
    22081 gattgaatta ggacttccta cgattttagc acgtatgatc gatgtaggaa tccgcaatga
    22141 tgattttgat tatgtgaagc aacaagggat atttatgatc gttattactg taatcggtat
    22201 tattttgaat atcttgttag gttattttgg ttcgaggatt acgactaata tcgtagcaga
    22261 tattcgagat gatttgttcg aaaagatcca aagttattcg catcaggaat acgaaaatct
    22321 aggcgtatct tcattgatca ctcgtacaac aaacgatgct tatcagatca tgttgttcct
    22381 gcaaaatatt ttacgtatcg gttttatggc accaatgatg ttcattgtca gtttgtacat
    22441 ggtcatgcgt acaagtgtga cactcagttt gtatgtagta ggtgcattac ctttactatt
    22501 attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa
    22561 cttagataaa atcaacagta tcttgcggga aaatctttca ggattgcgtg tgattcgtgc
    22621 attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga attatgcttc
    22681 tagttcaaaa agcttattcc gtttgatggc tgttgcacaa ccgggttttt atttcttatt
    22741 taatatcgta atggtattga tcatctggag cggggcagtg caaatcgatg caggcgagct
    22801 tcgtgtagga gatttgattg cgtttatcga gtatatattc catgccttgt tctcattcat
    22861 gttatttgct agtgtgttca tgatgtatcc aagagcagct gtttcagctg gacgtatcca
    22921 agaagcccta gatgccaaac cttcgattgt agaagatcca aatggtatat ctgatacaaa
    22981 aactaaaggg tatatcgaat ttcaaaatgt aacatttgct tatccaggcc attcgcaaga
    23041 gccagtgata cgaaatgtca gttttacagc ctctccagga gagaccgttg cttttattgg
    23101 aagtaccggt agcggaaaat ctacgttgat ccaattgatc cctcgctttt atgatgtgag
    23161 tgaaggaaag atcttgatcg atggagtaga cgtgagagat tatcggctaa gcaagttgcg
    23221 agataagatt gggtttattc cgcaaaaagc gctgctattt actggaacga ttgcagataa
    23281 tttgcgatac gggaaagaag atgctacgca agaagaaatg gaacgtgcag ctgagattgc
    23341 tcaagcttca gaattcattt ctcgaaaacc agatggattt aatgaacatt tatcagaagg
    23401 tggttccaat ttttcaggtg gacaaaaaca acgattggca atagccagag ccatcatacg
    23461 ccgtccggaa atctatattt ttgatgacag cttttctgca ttagattatc aaacggatgc
    23521 gaagttacgt gctcgtctga aaaaagaaac gacagaatct actgtattga tcgtagcaca
    23581 acgtgtggga acgatcatgc atgcagataa gatcatcgta ttaaatgaag gagaagtcgt
    23641 cggtatgggg acgcatcggg agttgttgga aaaatgcccg atttattatg atattgccgc
    23701 ttctcaatta tcaaaggagg aattagcatg aaaaaaacct tttcttcctt aaaacgttta
    23761 gctcgatata tcagaccgta tcgtgagact tttcttttag tgatcctatt tacagttctg
    23821 acagtggctt tcaacgtggc tttgccatat gttacaggac ttcctactac agaaatcagc
    23881 cgtaatattg ccaatggcga atcaatcaat ttttcttata tattcaaatg tctcatttgg
    23941 atcacagttg ttggaatagg gtattgtatt tcgcaattac tttctggtgt tttgatgaca
    24001 aatgtcgttc aaagtgccat gcatgatttg agaagagaca tagaagaaaa aatcaatcgt
    24061 cttccggttt catatttcga taaaaaccag caaggaaata ttttgtctcg tgtgacgaat
    24121 gatgtagatg cagtcagcgg tgctttacaa caagctttta ttggtatcgt aaatgcgata
    24181 ttgggaatta cgttggctgc agcaatgatg ttttatatcc agccgatgat ggcgctgatc
    24241 tctatgatca tgattccaac atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag
    24301 aaatattttc aaagcatgca gaattctctt ggtgagctaa acggctacgt ccaagaaaat
    24361 atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc
    24421 aaaaaagtta atcacagctt gaaatacttt gggtttcgtg cagcattcat ttctggcttg
    24481 atgatgcctc tagttcaatt aactgcctat gctacttata ttggtatggc tgttctagga
    24541 agcttttatg ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt catccaatat
    24601 atttggcaaa tcagtcagcc aatggggaac gtgacacaat tgtcttcagt tttgcaaagt
    24661 gcatcagcgg caacgaaacg tgtattcgag attttagatg agccagaaga aaaagaaaac
    24721 aaagtagatg cttcactgcc agaaacgatt ttaggagatg tgacattcga tcatgtcgat
    24781 tttgcatatg atccgaagaa accattaatc caagatttga attttgaagt gaaagctggt
    24841 caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga cgacattgat caatctattg
    24901 atgcgttttt atgacgtaga caaaggagca ataaggattg atggaatcga caccaaatca
    24961 atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc atggctttat
    25021 caaggaacga ttgcagataa tatccgcttc ggaaagcttg atgcgacaga ttatgaagta
    25081 gttgatgctg cgaaaacagc aaacgtagat cattttatcc gaacgatgcc agatggatat
    25141 gatatggcca tcaattctga gggagacaat gtgtctttag gacaaaaaca gttgctgacg
    25201 attgcccgag cagtggtctc cgatcctaaa atcttgattt tagatgaagc aacaagttca
    25261 gtagacaccc gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg
    25321 acaagtttcg tcatcgctca ccgtttatcg acaatccgtg atgcagatct gattctagtg
    25381 atggatcaag gtcagatcat tgaaaaagga acgcatgaga gtttattggc acaaggtgga
    25441 ttttatgaaa aactgtataa cagccaattt gctgaggaag cagaataagt catataagaa
    25501 taaaacagtt taaaacttat tttttgttct ctattcttga taatggtaaa gtaaagttat
    25561 caagaaaagt gaggacacgg agatggaaca gtttttaaat gaaaaaatta ctgaagccaa
    25621 aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt cagctgattt
    25681 ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt acgaattaat
    25741 tttaaaaaaa gtaatagaag aaaatataga tgtacaataa aaacaggtaa aacgtcagtt
    25801 accagatact gtaaatagac atctggcctg agcaggtttt acctgttttt tttcttatga
    25861 aataaatata tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg
    25921 agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca
    25981 ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata
    26041 aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca
    26101 cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca
    26161 atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt
    26221 gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat
    26281 gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa
    26341 aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa
    26401 ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt
    26461 tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag
    26521 attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt
    26581 gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc
    26641 cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg
    26701 caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct
    26761 cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca
    26821 ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt
    26881 cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa agatagatat
    26941 ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat
    27001 catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat
    27061 cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag
    27121 agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa
    27181 tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat
    27241 aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata
    27301 gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg
    27361 atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa
    27421 gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa
    27481 cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg
    27541 attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat
    27601 tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat
    27661 gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt
    27721 acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc
    27781 cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg
    27841 ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg
    27901 gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa
    27961 aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat
    28021 gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg
    28081 actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca
    28141 gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta
    28201 ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag
    28261 tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct
    28321 tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa
    28381 ccggagatac agctggactt attgatgagt acttctgata aaaagatcca actagcagca
    28441 gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc
    28501 caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg
    28561 gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt
    28621 cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt
    28681 ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag
    28741 atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc
    28801 aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa
    28861 cgttactcag ccgttgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac
    28921 cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat
    28981 gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg
    29041 ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca
    29101 gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta
    29161 gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac
    29221 ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc
    29281 gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa
    29341 gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa
    29401 acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag
    29461 ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat
    29521 caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga
    29581 aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat
    29641 ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca
    29701 aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt
    29761 tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag
    29821 acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat
    29881 cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc
    29941 tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg
    30001 cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat
    30061 acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg
    30121 caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa
    30181 tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca
    30241 attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat
    30301 tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt
    30361 ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag
    30421 ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat
    30481 gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt
    30541 cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc
    30601 gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt
    30661 gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga
    30721 agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa
    30781 atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DACCVU010000026        29783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCVU010000026 DACCVU010000000
VERSION     DACCVU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.026"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(10..288)
                     /locus_tag="IVW29_000995"
     CDS             complement(10..288)
                     /locus_tag="IVW29_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838494.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            481..1137
                     /locus_tag="IVW29_000996"
     CDS             481..1137
                     /locus_tag="IVW29_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7838495.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1579..2376)
                     /locus_tag="IVW29_000997"
     CDS             complement(1579..2376)
                     /locus_tag="IVW29_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7838496.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2373..3557)
                     /locus_tag="IVW29_000998"
     CDS             complement(2373..3557)
                     /locus_tag="IVW29_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7838497.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3582..4829)
                     /locus_tag="IVW29_000999"
     CDS             complement(3582..4829)
                     /locus_tag="IVW29_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7838498.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5044..6789
                     /gene="dnaX"
                     /locus_tag="IVW29_001000"
     CDS             5044..6789
                     /gene="dnaX"
                     /locus_tag="IVW29_001000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7838499.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6809..7123
                     /locus_tag="IVW29_001001"
     CDS             6809..7123
                     /locus_tag="IVW29_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7838500.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7148..7744
                     /gene="recR"
                     /locus_tag="IVW29_001002"
     CDS             7148..7744
                     /gene="recR"
                     /locus_tag="IVW29_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7838501.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7808..8452
                     /locus_tag="IVW29_001003"
     CDS             7808..8452
                     /locus_tag="IVW29_001003"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7838502.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8467..8796
                     /locus_tag="IVW29_001004"
     CDS             8467..8796
                     /locus_tag="IVW29_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838503.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8810..9748
                     /gene="holB"
                     /locus_tag="IVW29_001005"
     CDS             8810..9748
                     /gene="holB"
                     /locus_tag="IVW29_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7838504.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9784..10608
                     /locus_tag="IVW29_001006"
     CDS             9784..10608
                     /locus_tag="IVW29_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7838505.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10601..10948
                     /locus_tag="IVW29_001007"
     CDS             10601..10948
                     /locus_tag="IVW29_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7838506.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            11017..11889
                     /gene="rsmI"
                     /locus_tag="IVW29_001008"
     CDS             11017..11889
                     /gene="rsmI"
                     /locus_tag="IVW29_001008"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7838507.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11998..13119
                     /gene="dinB"
                     /locus_tag="IVW29_001009"
     CDS             11998..13119
                     /gene="dinB"
                     /locus_tag="IVW29_001009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7838508.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13173..13775)
                     /gene="nrdG"
                     /locus_tag="IVW29_001010"
     CDS             complement(13173..13775)
                     /gene="nrdG"
                     /locus_tag="IVW29_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7838509.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14090..16279)
                     /gene="nrdD"
                     /locus_tag="IVW29_001011"
     CDS             complement(14090..16279)
                     /gene="nrdD"
                     /locus_tag="IVW29_001011"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7838510.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16605..17372
                     /locus_tag="IVW29_001012"
     CDS             16605..17372
                     /locus_tag="IVW29_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838511.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17362..19404
                     /locus_tag="IVW29_001013"
     CDS             17362..19404
                     /locus_tag="IVW29_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838512.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19685..19834
                     /locus_tag="IVW29_001014"
     CDS             19685..19834
                     /locus_tag="IVW29_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7838513.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19845..21359
                     /gene="dltA"
                     /locus_tag="IVW29_001015"
     CDS             19845..21359
                     /gene="dltA"
                     /locus_tag="IVW29_001015"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7838514.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            21356..22561
                     /gene="dltB"
                     /locus_tag="IVW29_001016"
     CDS             21356..22561
                     /gene="dltB"
                     /locus_tag="IVW29_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7838515.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22606..22839
                     /gene="dltC"
                     /locus_tag="IVW29_001017"
     CDS             22606..22839
                     /gene="dltC"
                     /locus_tag="IVW29_001017"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7838516.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            22842..24110
                     /gene="dltD"
                     /locus_tag="IVW29_001018"
     CDS             22842..24110
                     /gene="dltD"
                     /locus_tag="IVW29_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7838517.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            24251..25333
                     /locus_tag="IVW29_001019"
     CDS             24251..25333
                     /locus_tag="IVW29_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7838518.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            25333..26136
                     /locus_tag="IVW29_001020"
     CDS             25333..26136
                     /locus_tag="IVW29_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7838519.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            26206..26946
                     /locus_tag="IVW29_001021"
     CDS             26206..26946
                     /locus_tag="IVW29_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7838520.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26957..27961
                     /locus_tag="IVW29_001022"
     CDS             26957..27961
                     /locus_tag="IVW29_001022"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7838521.1"
                     /translation="MIFVPNENHEPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(28006..29769)
                     /locus_tag="IVW29_001023"
     CDS             complement(28006..29769)
                     /locus_tag="IVW29_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838522.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9421 a         5235 c         6111 g         9016 t
ORIGIN      
        1 acattcccct taagctgaat atgcttttaa tttttgtatt tgaacttcta catcgtgata
       61 attggatgga tagcgatcaa caagttggcg aataaataaa tctcttgtag acataaacag
      121 atttaaaaat tcttcgcatt cgtttgttgg attctcatcc actgctttga tcaattcgat
      181 cgagttttga tacgcatcat ttaaaaagac taaagaagct ttcatatcaa atcttgtttg
      241 atatgctgtt tctaatcgat taatgattgt gttcatccag taattcatgt tttttccctc
      301 tattcttaaa aaattttact tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat
      361 aagtgatgga aaaaggtact taacaaatgt taatatcatt agtaaaatac ctttggactt
      421 taaaaagttt taaagtactg tatgatcttt ttagatgaaa gtttggatgg gggagcatca
      481 ttggatagta gagtcttaaa ggaatatttg gataaaaatg attttccgat tatacgcaaa
      541 ggatatcata aatatttgac attcgaaggg atggaagatt cccatacgta tatcttgaaa
      601 gagggaacgg taaaagcaag tgttatttca aatgatggga gagaatttaa ccttcgctat
      661 atcaaagatc ttgaaatcgt ctctctttta aaagacgaat attcacaatt catcgattcg
      721 ccgtataata ttcgagttga atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc
      781 ttttggcaaa atatcaacca agacccaaag ctacaacact atgtaaaaga gtattaccgc
      841 tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga
      901 gctgtttgta cgcaacttta cgaattatat gatttattcg gtgtgcggac agaagaaggg
      961 atgctgatcg atttttcgat cacgaatgaa gaaatcgcca aattttgtgg catctcaact
     1021 gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag atggtattat caccgttaaa
     1081 gaccatcata tcgttatttt gaatatggac ttgttagaag acaatattat cttttgatga
     1141 acaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata
     1201 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
     1261 taccaagagg ttgtgagcct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
     1321 agcttttcat gtttgatgaa tattgtgact tgtgaccgag gagttggtag gagaacaccg
     1381 tttaccaaga ggttgtgaaa aagctgttct gcatcaagta ataagaagag ccaagtaaaa
     1441 acagtctctt atgtttttaa cttggctgga cttatcaccg tagatgtaag cttttgaacg
     1501 ccgtttattt gcaacaaaag gggctgtgac acgatttttg tcacagccct ttttttgcga
     1561 acattcattt agcaattttt agaaacttct gccgccaccg ccaaaagtcc cgccaccgct
     1621 agaatgggta gtacttccgc caccgccgct tccaggaggt gggcttttgg ggattcttct
     1681 tgtcgtgaca aaagaatttg tcagacgatc ggttttttca gttaatttga tactggattt
     1741 ttctcggaat ggatatttgt agatgcctga tttcaattga taacgcgaaa cagtcacaaa
     1801 ataaaagaca agactaagga taagagccaa aacaacagca atgatgattt ctactgtcgt
     1861 caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct acacgataat gtcctcctgg
     1921 aactccgtct tccacatatg ttgctgattt agacaggtaa gcttttgcag cattgtaata
     1981 ggcagcattt gtcatctgat cataaacatc atctaagatc gaatcgattc tgctatcagt
     2041 caaataatcg atcatattac cagaagtcga aatgtagatt tcacgctgtc ccatatctaa
     2101 tagcaatact gagccatttt gatcatttcc gactgcgtct cttaagtaat tatcagcaaa
     2161 gtctcttggt tcttctgaat tagatgtagt cgttacgatc aagactcgtc ctttgatttt
     2221 ttcattcagc ttttctgcct gttgttccaa agattggatt tgatctgttg tgaataaccc
     2281 agcctcatca tttaccgtag ctgtatctgc atgagtagcc accggccagc caacacttac
     2341 tagcaaaaga aagaataaaa aaaatttttt catcatatca agtaacctcc gatcagaaag
     2401 aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc ccaatttttt ctggctgatc
     2461 ggcaggacac cgctgacctt tcctgtttgt ccattcattg cgtagtagta gactttttta
     2521 cttgagtcat tgcttcgata agtaacaagc cataccggca gtaatacgta attatttact
     2581 tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg ttgtatatcc atttgcggta
     2641 tcttttaaca gcttctctga atagtctttt aattcagatt gaatctgtga tttgattgcc
     2701 tcatattcga tatctctttt ttctgcttgg aacccggcta aatactggct tttaaaatca
     2761 attgctttat ctaacgggaa aggttgcacg ccttcgacca tcttttgttg cgtatttttt
     2821 gataaggcat tttttactag ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat
     2881 tgcttcgttt ctgtgtattc gatgtcacct atccgccata tgcggataac tgtaccattc
     2941 gcctgcatgg caccatctac ttcagcatct gtagcccaat aagggaaata cacacctgtc
     3001 aacttatcga tttgatcctt attgaaaaaa gctttgggaa caaaccattt tttcttggtc
     3061 caagcgagaa atctgttgat tgcttcttct ttctcgacag caaaaggaag tactttattt
     3121 ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg gattatggca gtagtaacaa
     3181 taagtcgcag cagttgtcgc atcagtcacg atctctgcac cacagttggg acaaagaaaa
     3241 agatctatcg ctgcggtatt atctgcagat tcgtccgctg tatctgaaaa accgcctgcc
     3301 tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa
     3361 gtaaaatctt cggtaggtga aggttcttct ttgctttctt cgccagccat acgcgcttct
     3421 ttttgctttt gctcatattg gctgacttct tcttctgtat agatattcag gcagtattcg
     3481 caatgaaatt tttggtcttt gggatcaaaa gtcaacggac cgccgcaatt aggacattta
     3541 tgtgtaagaa catccatcag ctgcacacct ttctaaataa attaattaac ggggattcct
     3601 ttaaacggtt taccacagtt tggacagaat ttagggatgc cgttggaaag atccacgatc
     3661 tcattgcatt cactgcatcg catttgtagc ttaggctgaa cgttttctac aggtttggca
     3721 gctccgcagt tggaacagaa ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa
     3781 gtatccgcat ttccttgtgc agtttgttga ttttgttttt ctgcttgttg tttgatttgt
     3841 tcttggttat tttgagctgc ttgagaaaga taagaaccat ttgcattcat tcccatacct
     3901 acacccataa agccggtcat tgctcctgct tcattttttc cggctgcttc cataccacgt
     3961 gcaatggaac cttgcacgta tccttcacgt acgctaggat cacctagcat cgccccttcg
     4021 ttacgcatat tgatcagttt cacagaatca tcggtgtaag aaatgctggc tacagccaca
     4081 gaaacaatct ccatcccacg taattcacgc caagaatcat ccaatgcagt atccatgtat
     4141 ttgcttaatt ccaaactctt agatggtaca taagaaatcc gttgaccatc tgcagacatc
     4201 tgattgattg ctgattgtag cgctgttagg aattcagcta aatactgctc attgatatca
     4261 ttgatttcca cttgtgtttt gtttttaggg attgcgtttg tataaaataa aatcgggtca
     4321 acgacatgaa tagaataagt gccatgtgcc cgtaagaata attctgcgtt atagaaattg
     4381 tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc cctttatttc ttggagattg
     4441 atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt
     4501 tcagcaattg cttctttcag tgttccattg aacatggaag gagctgcgtc atttttaatg
     4561 gtgtaataac cttcctcagc ggtataatcg atgatttttc caccatcgac caaaagcatc
     4621 atcatgtttg gatagacatg gacgactgtt ccgtctgtta aaacatcttc tgttccttta
     4681 cgattggagc cacgtttgtc atctttgcgg actttgacac cttttgtcat gaccgttgta
     4741 tcactcatat tgtctggttc gatgacttct agccattgat ctgccagacc gccgccaacc
     4801 atacttgttg ctgctttgat aagacccata aaaaattcct cctctgattc agctagtgcc
     4861 aaacaattat tgtttattta ttataccata ggaagatgtg aaacttctcg gactagagac
     4921 ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag aaggatagta
     4981 gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa aagggggatc
     5041 cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt
     5101 ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa aacctcacat
     5161 gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa gattttcgca
     5221 aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt
     5281 cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac
     5341 ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg
     5401 tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt caatgccttg
     5461 ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac gaccgaacct
     5521 cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa acggatcaat
     5581 acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat cagctatgaa
     5641 ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga tgcattgagt
     5701 attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga tgccatgcaa
     5761 gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa
     5821 gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa agaatcacgt
     5881 cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca gcaagcacct
     5941 aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt ggcacaaact
     6001 gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca aaatgaaatt
     6061 cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact tgcacagcta
     6121 ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat agtaaaacag
     6181 ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag taatacagga
     6241 attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca gacaagtacc
     6301 tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag acagcatctg
     6361 aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac tcagcgagca
     6421 atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat tgcgtttgat
     6481 tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc ggtacacaac
     6541 aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac aagagaaagc
     6601 tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac agaaaactct
     6661 tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca agaagtacca
     6721 gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt tacagtggta
     6781 gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata tgcaaggcat
     6841 gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa
     6901 aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta caggcgatcg
     6961 tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata tcgagatgct
     7021 tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg tggaaactga
     7081 gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa ctgaaggagt
     7141 aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca tgaaacttcc
     7201 aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata tgaaagatga
     7261 agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag
     7321 tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga
     7381 tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg
     7441 agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg aagggacagg
     7501 tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag atgtaaaaga
     7561 agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt atctctcacg
     7621 tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt ctgtaggctc
     7681 tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa gacgtgagtt
     7741 ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa gagatggggg
     7801 caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag gaaaaaccag
     7861 catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa tcattcaaac
     7921 aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc ttgatccaaa
     7981 aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa gaagacaaca
     8041 tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat gcgatagatt
     8101 tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta ccgagattgc
     8161 tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt atcttgacgt
     8221 agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt
     8281 agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa agctggcaga
     8341 agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag aagtgctgca
     8401 aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact aggaaggtga
     8461 acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg tttatcaaat
     8521 gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg cttccttaag
     8581 gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga agcattggaa
     8641 atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt tacactggat
     8701 atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg tgccacagtc
     8761 ttcgtcttac ctgttgaagg attccatcaa ttttaggagg agctgagcta tgaatcaaga
     8821 aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga gttttgagca
     8881 tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa aacatgaagt
     8941 aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc tgccttgtgg
     9001 aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt tggttattga
     9061 accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg aattcagtcg
     9121 cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag
     9181 cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt ttttagcggt
     9241 gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt gtcaagttct
     9301 gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag agcagattcc
     9361 attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag cagttgaaat
     9421 ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt tcgtttactt
     9481 gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa aatcatttaa
     9541 agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc aggaagcaca
     9601 gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc agataattga
     9661 acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg ctgttgcgga
     9721 acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc aaggaggatg
     9781 gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta ttattttgca
     9841 ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca gcaatcctta
     9901 catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct tcctgaagct
     9961 atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa
    10021 aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa
    10081 atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt ttctttcaca
    10141 gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt atttcgtact
    10201 cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg cggaattggt
    10261 ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc
    10321 aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg tttatgcgga
    10381 cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa aaaagaactc
    10441 cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt agggttgaat
    10501 ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt cgaatatgaa
    10561 tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga gatcattata
    10621 cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac ttgttgaaat
    10681 gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg
    10741 tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg aaaatgtgaa
    10801 gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag gattccatgt
    10861 ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt tttgtctaga
    10921 agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga gcggagaaat
    10981 actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa aagtttcaag
    11041 ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa tttagaagat
    11101 atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg
    11161 cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa aagtttacat
    11221 gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg
    11281 attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca tgagctagtt
    11341 ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac agctgggatg
    11401 acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg cttccttcca
    11461 cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac agttacgctg
    11521 attttttatg agtcacctta tcggatcact gctacattga cgaatttctt agaaattttt
    11581 ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga
    11641 ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt
    11701 ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga agagttggga
    11761 acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc
    11821 aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa
    11881 ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat cagctactaa
    11941 cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga ggtgaggatg
    12001 atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca tattgatatg
    12061 gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg acatccatta
    12121 gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac agcaaattat
    12181 gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta tgagctttgt
    12241 ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc caaacaagta
    12301 cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga tgaagcgtat
    12361 cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc
    12421 caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag ttacaataag
    12481 tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt agttgcccct
    12541 caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg ggtagggaag
    12601 aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct atatgaatgc
    12661 acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta tcgtaaagtc
    12721 agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt gggaaaagag
    12781 catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta
    12841 gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac agtagttttg
    12901 aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc agaatatatc
    12961 tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat cttgggagta
    13021 gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat gacttatgaa
    13081 aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg agagttgaaa
    13141 ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt tttgatttgt
    13201 tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt ggattctaaa
    13261 gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag ttttaggtca
    13321 cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag gagttctaat
    13381 ttatcttctg aatcttgtag caattcatca aaggtatagc ctgtccatga ccagatatct
    13441 tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac atctgtattc
    13501 aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg caagtcagcg
    13561 atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct
    13621 ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact gttgcggaca
    13681 ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg gctgtagtct
    13741 tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc atttaaggtt
    13801 caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa caaactgacc
    13861 taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca aagatacaaa
    13921 gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg aataaacggt
    13981 gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg aggaactatt
    14041 tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct catttcatgt
    14101 gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga gcttgcggat
    14161 ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca tgatttccac
    14221 attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa ccacattgat
    14281 agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc
    14341 atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag tagtggatga
    14401 atccacctga acaatatttt ggataatcct tctcaaattc caatttttca aatggcgttg
    14461 ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct gtgatatttt
    14521 cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga ctttcacttg
    14581 gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct gcattagctt
    14641 ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc
    14701 caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat acagtagctc
    14761 gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc ccgaaagcac
    14821 catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt tctacacgat
    14881 agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag aatttattct
    14941 gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca cctaaattca
    15001 ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag aatgaacgac
    15061 agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa ttcagaatat
    15121 caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg taattaggat
    15181 cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg aagatagctg
    15241 tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt tgaatttctc
    15301 tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg
    15361 aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag atatcttttt
    15421 tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat tcttccgcgt
    15481 cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca tctgttcggt
    15541 cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt gagatttgtg
    15601 ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt ttgaacccat
    15661 tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta taaggatgat
    15721 aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata tgtggtggca
    15781 acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc tgtgtattga
    15841 aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg ttgattagct
    15901 tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt ttggtacgat
    15961 aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata tgttccacga
    16021 tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt tcttggacaa
    16081 ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag ccgtgaattt
    16141 tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca acatggcggc
    16201 catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg acagtttttt
    16261 cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc tatcataaaa
    16321 caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg tgctgtataa
    16381 ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga aaacttgtag
    16441 ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga ataaaatttt
    16501 ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta gatatgctac
    16561 tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa aagatgatcg
    16621 aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa gccttagata
    16681 atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca agtggtgcag
    16741 gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg ggcagtgtct
    16801 cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa
    16861 aaaaactagg attcattttt caagagttca atttgatcga taccctaaat gcaaaagaca
    16921 atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa aagagagtaa
    16981 gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc gatgaacttt
    17041 ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag ccacaagtga
    17101 tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa ttgttgaact
    17161 acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc catgatccat
    17221 atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt ttttctgaag
    17281 ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg caggcgacga
    17341 ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt ttttcggcag
    17401 tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat ctattattcc
    17461 ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga tacttcgata
    17521 gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt tttcgtattc
    17581 agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta tcagcttttt
    17641 ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat aattggattg
    17701 ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt ttcaatgatt
    17761 ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat tccatcagta
    17821 gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata ttcaatatgg
    17881 aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc agatagttca
    17941 acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact acttttaacg
    18001 agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat cagttatcat
    18061 tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg tgtggtcggt
    18121 acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag taaatggaag
    18181 atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca tttgctcaaa
    18241 agttggcgta caaattctct gattacagtc attctcggaa tttctttaat gacaattggc
    18301 ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc cccgatggac
    18361 tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga ggaagggcag
    18421 aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta tagtcaaaat
    18481 ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata tcaagccttc
    18541 agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca tactgttttg
    18601 cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag tattggactg
    18661 ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg tgacgaaaca
    18721 atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa agcaaaaggt
    18781 gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa
    18841 cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc ttatgatatc
    18901 gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga ccaaatgaag
    18961 gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta ttcgaaattg
    19021 cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt cgggatgatc
    19081 gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc agaggaagaa
    19141 aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat cagacggatg
    19201 atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat cactcacgca
    19261 gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg gttggcttat
    19321 cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac gagtcagtta
    19381 tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa tagaactaaa
    19441 ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca caacctcttt
    19501 attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg
    19561 ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag
    19621 acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa
    19681 tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg
    19741 aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat
    19801 tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga
    19861 ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg
    19921 atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag
    19981 aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga
    20041 ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata
    20101 cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta
    20161 tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag
    20221 aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt
    20281 tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg
    20341 ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc
    20401 atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat
    20461 tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat
    20521 tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg
    20581 atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt
    20641 tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa
    20701 atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc
    20761 agattgacca gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag
    20821 acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa
    20881 tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg
    20941 ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag
    21001 atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat
    21061 accggattga actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag
    21121 ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat
    21181 tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac
    21241 tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc
    21301 agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat
    21361 gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct
    21421 tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc
    21481 agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc
    21541 gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca
    21601 aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt
    21661 tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg
    21721 aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat
    21781 caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc
    21841 tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga
    21901 taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa
    21961 attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt
    22021 acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt
    22081 gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata
    22141 tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa
    22201 tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt
    22261 cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt
    22321 tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg
    22381 actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca
    22441 taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca
    22501 agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta
    22561 acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt
    22621 ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat
    22681 ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac
    22741 ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg gagcacaccg
    22801 aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg
    22861 ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct
    22921 atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga
    22981 acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt
    23041 tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa
    23101 aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc
    23161 aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta
    23221 tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt
    23281 ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct
    23341 atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga
    23401 ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca
    23461 gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc
    23521 gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat
    23581 tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga
    23641 aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa
    23701 aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat
    23761 tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg
    23821 attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat
    23881 taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt
    23941 ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca
    24001 gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt
    24061 ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg
    24121 cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc
    24181 aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg
    24241 gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg
    24301 agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc
    24361 tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg
    24421 ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac
    24481 cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc
    24541 tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa
    24601 accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt
    24661 cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga
    24721 tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa
    24781 agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa
    24841 agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg
    24901 aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg
    24961 tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg
    25021 cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt
    25081 tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg
    25141 aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt
    25201 tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac
    25261 agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg
    25321 gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt
    25381 tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg
    25441 agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag
    25501 cacttattga gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt
    25561 tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt
    25621 ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac
    25681 tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat
    25741 atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt
    25801 gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt
    25861 attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg
    25921 gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt
    25981 ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt
    26041 cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg
    26101 attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa
    26161 aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt
    26221 ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa
    26281 tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa
    26341 caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt
    26401 gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca
    26461 ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa
    26521 aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg
    26581 attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa
    26641 acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat
    26701 tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat
    26761 catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag
    26821 aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg
    26881 caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac
    26941 atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgaaccaa gaatcaattt
    27001 agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta
    27061 tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg
    27121 agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt
    27181 ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt
    27241 ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc
    27301 tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa
    27361 cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga
    27421 tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat
    27481 caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa
    27541 aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt
    27601 agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc
    27661 tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc
    27721 tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat
    27781 gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga
    27841 aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat
    27901 tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg
    27961 aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata caggattcgg
    28021 gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag
    28081 tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc
    28141 atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc
    28201 gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag
    28261 tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg
    28321 agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg
    28381 aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg
    28441 gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata
    28501 gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac
    28561 aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg
    28621 ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt
    28681 taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat
    28741 cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca
    28801 atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt
    28861 atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt
    28921 agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc
    28981 gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat
    29041 tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa
    29101 aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc
    29161 aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg
    29221 tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat
    29281 catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt
    29341 tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag
    29401 ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg
    29461 attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt
    29521 aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt
    29581 cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct
    29641 gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt
    29701 catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa
    29761 aaataacaat gataatataa tga
//
LOCUS       DACCVU010000027        27747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCVU010000027 DACCVU010000000
VERSION     DACCVU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.027"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(286..1230)
                     /locus_tag="IVW29_001024"
     CDS             complement(286..1230)
                     /locus_tag="IVW29_001024"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7838523.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1526..2470)
                     /locus_tag="IVW29_001025"
     CDS             complement(1526..2470)
                     /locus_tag="IVW29_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838524.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2691..3905
                     /locus_tag="IVW29_001026"
     CDS             2691..3905
                     /locus_tag="IVW29_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7838525.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3927..5942
                     /locus_tag="IVW29_001027"
     CDS             3927..5942
                     /locus_tag="IVW29_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7838526.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6025..7398)
                     /locus_tag="IVW29_001028"
     CDS             complement(6025..7398)
                     /locus_tag="IVW29_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7838527.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7424..7708)
                     /locus_tag="IVW29_001029"
     CDS             complement(7424..7708)
                     /locus_tag="IVW29_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7838528.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7710..9725)
                     /locus_tag="IVW29_001030"
     CDS             complement(7710..9725)
                     /locus_tag="IVW29_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7838529.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9904..10209)
                     /locus_tag="IVW29_001031"
     CDS             complement(9904..10209)
                     /locus_tag="IVW29_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7838530.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10298..11329)
                     /locus_tag="IVW29_001032"
     CDS             complement(10298..11329)
                     /locus_tag="IVW29_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7838531.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11536..12411
                     /locus_tag="IVW29_001033"
     CDS             11536..12411
                     /locus_tag="IVW29_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7838532.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12563..13405)
                     /locus_tag="IVW29_001034"
     CDS             complement(12563..13405)
                     /locus_tag="IVW29_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7838533.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13497..15374)
                     /locus_tag="IVW29_001035"
     CDS             complement(13497..15374)
                     /locus_tag="IVW29_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7838534.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15371..16792)
                     /locus_tag="IVW29_001036"
     CDS             complement(15371..16792)
                     /locus_tag="IVW29_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7838535.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16795..20184)
                     /locus_tag="IVW29_001037"
     CDS             complement(16795..20184)
                     /locus_tag="IVW29_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7838536.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20441..21832
                     /locus_tag="IVW29_001038"
     CDS             20441..21832
                     /locus_tag="IVW29_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7838537.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21905..22552)
                     /locus_tag="IVW29_001039"
     CDS             complement(21905..22552)
                     /locus_tag="IVW29_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838538.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22560..23246)
                     /locus_tag="IVW29_001040"
     CDS             complement(22560..23246)
                     /locus_tag="IVW29_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838539.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23252..24325)
                     /locus_tag="IVW29_001041"
     CDS             complement(23252..24325)
                     /locus_tag="IVW29_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7838540.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24547..25221
                     /locus_tag="IVW29_001042"
     CDS             24547..25221
                     /locus_tag="IVW29_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838541.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25226..26566
                     /locus_tag="IVW29_001043"
     CDS             25226..26566
                     /locus_tag="IVW29_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7838542.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26715..27701)
                     /locus_tag="IVW29_001044"
     CDS             complement(26715..27701)
                     /locus_tag="IVW29_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7838543.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8087 a         5244 c         5035 g         9381 t
ORIGIN      
        1 tttaaaaaat gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc
       61 aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt
      121 ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat
      181 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
      241 tgctgttaag cagttttagg atttttagtt catattcatt gtttcttata atacagtgtt
      301 caatacttct cttaaagcac ttgttgattt gctgaacatt gctttttctt cttcgttgat
      361 tgataattca acaacttcac gaacaccatt ttcgtcaacg attgatggca cacctgtaaa
      421 gatatctttt tcgtcgtatt caccagttaa ataagcagat acaggcaata cagcttgttc
      481 gttattcaag atagctttta cgatacgtgc agtactcata ccaataccat agtaagttgc
      541 ttttttacga tcaatgatct cataagcagc attacggact ttatcagcaa tcacatctaa
      601 ttcatcttgg gcaatacgat ggtctttttc tacaatttca aacactggtt taccaccgac
      661 agttgtatgt gaccatgcag caacttctga atcaccatgt tcacctaaga tatagccgtg
      721 gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct ttacggaaac gagttgtatc
      781 caatgttgtt ccagtaccga taacacgtga tgttggcaga ccagattcgt tccaagcgat
      841 atatgtcaaa atatctactg ggtttgaagc aacaacgata ataccgtcga atcctgatcc
      901 catgatttct tttacgattt ggcgcatgat tgacgcattt gttttaacta aatctaaacg
      961 agattgacca ggtttttggt tgataccagc tgtgataaca acgatatcag catctttaca
     1021 ttcttcatag ccacctgacc atacagccac attttcatcg ccccaagcca taccatctaa
     1081 aaggtccaat gcttcccctt ctgctttttc ttgattcaca tcgatcaata ctaattcatt
     1141 agagattcct tggttgatca ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac
     1201 gattactact ttacgacttg tttttttcat gaataaacag ctccttttca taactatttg
     1261 ttactttcat gaaaagtata gtcttgttta ttcattttat caacatgtta attataatag
     1321 ttgttattat aatttttgat ggaaacgaat tcctgaatac gcataacaga taaatgacaa
     1381 atgtttacac ctatatcccc tgataataca accaaaagtt tagaactgaa aataaatcca
     1441 aaaaaattcg ctgcaacgat ataataagac agcaatcgca cctaaaacaa ttttccttca
     1501 tatgctttag tgaaagattt tcttcttaat gtttatttct ccattcattg ggagtacatg
     1561 ttgaatactt cttgaataat ttgaataaag aagtcgtttg tttatatccg atacgattag
     1621 cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc caacgcaaag tcgaattttt
     1681 tctttagaac cagttcttga aaggaagcat caaaatacat tttcaattta ttactaagat
     1741 agaccggatt gtagccaaaa tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct
     1801 tatcgatata atctaatact ctaagcatct cattttctgc gcatccatct gctgatgaaa
     1861 agctttgttg ttctaataaa tgagtcaatt cgatcagtaa tagtgatgta tagagattta
     1921 acgaacggat cttatgactg tatgtatcaa aataaatcaa cagcatattt tcaatcaaat
     1981 gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt
     2041 cttttgatgc atttatcaaa aaagaagaaa gtagattatt tgtgcttgcc aaatgattca
     2101 atatctctgt agataaggtt tcttctttta ataagatgtt gattaaaata tcctcttgcc
     2161 ctgtgtattc gattctttga gtagcttctt tatcaatgat caccacgtca ctttcttcca
     2221 tctctatcct ttctccatta ataatttgta aacatgtacc gctatacata taattgattt
     2281 caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg acggtgttta ctgatatgaa
     2341 tatttccctg ttgaaataga tcccctcggt ggatttttgg cggtgtctga agatggtaag
     2401 ctaagtttaa atcattgata ttcattcctt gttcacgctg cagagtttcg attgattctt
     2461 cgttacgcaa aaaaaatgtc taattcagct agttccaatt gactccctcc tttttgtaag
     2521 cggtttcttt cgcttattag ttcacgctgt actgctatta tatgcaaagc tatttttggt
     2581 ttcataaaga ctttctatgg ttgggacatg aacttcctgt gattccgcta ttgagagaat
     2641 aaccacttca tactatattt ttgataagcg cttacaaaaa tgaaaggaac atgcttatga
     2701 ataaaaagat gatgaaattt tctctgctgt cagtctctct tattttgact tctgctggtg
     2761 cagtcacgtc tgcaattccc agtatgtcag ctacttatgc cacgcgttct ttatctgctg
     2821 ttgaattatt aacgactgct ccctctttta tggtcactat ttttgttttg attagtagtt
     2881 ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat cggattagtg attgcatcga
     2941 tttgtggtat cctccccctc ttctcacaaa gttacagtat tgtcttagct tcaagagctg
     3001 gactaggtat tggctttgga ttgatcaatt ctttggcagt cagcatgatt gctgcctttt
     3061 atcacggaga tgaacgagcc tctatgattg gttttcaaag tgcatttcaa ggatttggtg
     3121 cagctttaat gacatttgtt gcaggacagc tagtacagtt cggttggcaa tatacctttt
     3181 tagtctacgc catcagctta cctattcttg tcttgtttgt tttgtttgtt cctacacctg
     3241 aaacaactga acttcaatcg catggcacag ccccaacaag acaagtaatc aaaacgaatc
     3301 ctaaaatgat tagttatggt atttttctct tgctggtcat cgtggtatat aatgctgtat
     3361 cgattaaatt agccacagtc ctgacgactt attctctagg aaacgaaact aatgcagcga
     3421 atatcttaac aatcatgcaa ttggcaagta tggctgccgg agtttgtttt ggctggcttc
     3481 agacaaaagc acatcgctat atgcttacac tttctttact actgatggga atcggctttg
     3541 tattgattgc cactgtgata aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa
     3601 tttctttttc gttattcatt ccttacttat tcaaccaagt aagtatccaa gcgcccaagt
     3661 acgcggagag tttcaatact tcaatcttat tggttggcgc taatttaggt actttcattg
     3721 caccatatgg tttgctcttt ctctcttttg cttctcttgg gagtaaaaca cttgctgttt
     3781 tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat tagccttcta gtagtacaca
     3841 ggacgaatag aaagacagtg ataagagtag atgaagaaat aaaagaacaa ttgacaacaa
     3901 aataaatttg atggaggtcg tgtaaaatga caaatgaaaa acataaagat agatggcttt
     3961 ggtatcctgg agactttgag atccgccatg gactgctaca aaatttccaa cgggaagaac
     4021 gaggttttga ctggccagcg tactggtaca tggatgattg ccatcggaac gtcaagttta
     4081 agagatatta ctttcttgat caaccttcca tgtttaaagt gacgatccaa ggagttgggt
     4141 atgtagaaat taatggacaa aaacatccat gtggaaaatg gctgacttgt ccagctggta
     4201 aagcaaaaat tcgtatcttt gtaggacata catcagggct accagcgatg tttatcgagg
     4261 gagacgaagt aaaatcagat attggctgga cagcaagtaa ttttattgaa gaatatccag
     4321 ctggctggag tccgctttat gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa
     4381 aagaatgcgt ccatccagtc actgaaacgg agattggcgg aggaatctta ttcgattttg
     4441 ggcgagcagt gaatggctgt gtagaactaa tatctctgga tgaccatatc gaatctatca
     4501 ctctatgtta tggagagtcc gatgtagaag ctatggatat cgattattgc tactataagc
     4561 aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac
     4621 cagaggtttc tactggaatg gttcaacttc aagtttttca tgaattttta tctctttcga
     4681 aacaagctgc tttttcaagc gattcttctt tactgaatca gatctggtca gtatcagaag
     4741 aagccttcaa gttatgtagt gatttgtttt ttatcgacgg tatcaaacga gatcgctgga
     4801 tttggtctgg agatgcgtat caatcattca tgatcaatca atatcttttt tttgacgagg
     4861 aaatcaataa acgaacgata ttagcattaa gaggacaaaa tgaaatcaag cagcatatga
     4921 atactatcgt tgactattcg atcttgtggt tgattggtat cgaaaaccac tatatgatga
     4981 cgaaagacta tgaattttta cgcatcgtct accctaagat gcaatcatta atgagatact
     5041 tgatggagca aacgaatgaa ttagggttta tttatgggag agcaaaagat tggatattta
     5101 ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc agaacaagtc ttattactga
     5161 aagcctatca ttccatgatc acttgcggag atatcttagc tgaaacatat ggagagaacg
     5221 aaaattatca tactcatctc tatcaagaaa aatatcaact attgaaacaa aacctatttg
     5281 actacttttg ggatgaagaa cttggcgctt ttatcgattg ctatgaatct gggagaagaa
     5341 atgtgacgcg tcatgcgaat attttcgctg ttttattcga ttttgctaac aaagaccaac
     5401 agcaatcgat cttaaagaat gtcttattga atgacactat cacacagatt actactccat
     5461 atttcaaatt ctatgaacaa gatgctttat gcaaactcgg acagacgaat atcgtctacc
     5521 aaacgattct tgattattgg ggagggatgt tatctcatga tgcggtaact ttctgggaag
     5581 aatacgaccc tactcaaaca ggaaatgaaa agtacagtat gtatggagat ccttatggaa
     5641 aaagcttatg ccatgcatgg ggagcaagcc caatctatct tttaggacgc tattttatcg
     5701 gtttgtatcc aacaaagcca ggttatgaag aatttatgat acaacctaat ctagattcat
     5761 ttgaaacact gaattgtacg ctacctatca aagatggaaa gatttcgctt gaaaaaacaa
     5821 aagataagat cgtgattact tctgatagaa caggcggaac agtgaaaatg aatggtaaaa
     5881 gttatcctct tctagcaaaa cagccacttg tcttaacaag ctattcaaaa ataaatgcat
     5941 aaaacaaaaa actgcttgcg acatatcaga agactttaaa tcttcttctg atattgtcca
     6001 agcaattttt attttgaagt ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt
     6061 tccggcaaaa agaaagctag ataatacgac aagtaaaaag aaagcgatga ttccaactgt
     6121 caaggctatt tgaccgtatt ctcctagttt cccaagtaca agtcctggta ctagataatc
     6181 agtatcggca aatgtagaag aagcgatttt aagatctcct aatactggaa ggataaataa
     6241 tggcagccaa ctaatcaata agctattgaa agcagaacct aataccgccc ctcttctacc
     6301 gccagtcgag ttgccgaaga caccagaagc agctccgcag aagaaatgtc ctactacccc
     6361 aggaatgata actgtagttt gcaagcctat catagcaaac atggatagtg ttcctacaaa
     6421 gaagctaaca aaaaagccaa ttaatacagc attaggtgca tatggaaaaa cgataggaac
     6481 atctaatgct ggtttagaat cgggtacgag ttttttagca atcccttgga atgctggaac
     6541 gatttctgcc aacaccattc gaaccccttg aaggacgata acaaacccag ctgtgaatgt
     6601 tccagcttgg atcagtgcaa agattacagc gttgtctcca ttgcttagtt ctctttcaac
     6661 aaatccagga ccagctaaca aagcaagtgc gacatatacg atcgacatca agagcatgat
     6721 cgatacagta gaatctcgca aaaagctgaa acttttagga atctttatgt cctctgtaga
     6781 acggtctttg tttccaaaaa actttccgat ccatccacta gaagcataac cgatatttcc
     6841 ggtatgccct aacgcaactt gattgtttcc agtgatgttc ttcataaacg gttgacaaag
     6901 agcaggcgta atagttaata gtgttccttc gaagaccccg cctaataaga tcatccacaa
     6961 atttgcagta aaccccgcgc ttactaaaat gactgctgtt agacaagaaa cataaagcat
     7021 tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat ctagcaagta aaatattcac
     7081 gatcatccct actagcatga tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc
     7141 cactactgct tcattgctag gaactactcc ttgagtatgc agagctgttt ggaacatcga
     7201 agcaaaaggg gttagtgaat tactgattaa accagcaccg ccgatcagta ctaggaatcc
     7261 gacaaatgtt ttgatagttc cttggatcac ttctgaaaat ttcttcttct gaagaacgag
     7321 accaagcagt gcaatcaaag aaacgaggat ggagggctga ctgacgattg acacaaacaa
     7381 attcaacaat ttatccattg atagttcctc ccttgaatca tttttattct tttgacaaga
     7441 tcttcgttac tttttctttt acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct
     7501 tttcatcagg tatagaagtt agcgtatctt tcaaagaatg ctcaatgaag aaataatcag
     7561 ccatatcgct agatacactt ccaacatccg tatgacttac ttcaaactcg tccacatctg
     7621 ctccaagatc tgcaagtgct tgtttgatgt tcatctctac cataaaactt gttcctaatc
     7681 cactttgaca tactgcaaaa attttcatat cattcgtctc cttttcttat gatcgctaat
     7741 atttcttctt ttgttgatgc gtgtaataaa tcttgaagat tttgtttatt agaaagtagc
     7801 tttgttaaat ttgccaaagc ccgaagatgt gcctcattat cgatagcagc taaacagatg
     7861 aacaaactaa tgggatgggc tggattatca agcagatcca caggttgttg aagtttcagc
     7921 aaagacatcc ctacttcgtt tacgccatct tctggacgag catgaggtaa tgcgatgtga
     7981 tcgccaatat ggataaacgc accatactgc tccacgcgct cgatcatagc tttgatataa
     8041 ctttcttcta ttttattttc tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc
     8101 cagttttttg gttgatccga taattggatc atttcttctg ttaataattc agatagcata
     8161 ggacgttgat cctccttctt ttctaaaagt ttgttcattt tacgatttaa tacttgatag
     8221 agcttttctt cgtctacgcc ttcttttaat tcgatataag gcagtagcgc cgacaacaat
     8281 tcttgggcag aaggcattga aaatcctggt atcagccaat ctctttgtac ttgattgatc
     8341 agctgatttt tttccaagtc agtcatgatt ggatgcagaa catagagaga ctttttctta
     8401 tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg
     8461 tgtaagcggt cagctgaatt agtctctgta aaaaagatca tcgggaataa ttgctgaagt
     8521 tcacttttta agatcaatga agagctaatg ccacttggac agacgatcac ggctcttatt
     8581 tttctatttc tttcttctgc gtccgctttt ctgatctctc cgccaaatag tatagtgaaa
     8641 aaaccaatct cttcttcagg gatgctcttc cctattcgtc tttctaacgg aagtaatgct
     8701 tttcttgtca tttcgtagat ggaagcatat tgatgcttaa tttgatcgat catcacatta
     8761 ggtaaataaa aagaatagga aatacgaaaa taggcaggga ccaagtgata aaagacattc
     8821 atcaataatt cacgaaacga agcaaattct acggccatca ggaattccat gcgatggatg
     8881 acttctgaag aacattccag caaaaaatca agtgcaggct cctggacatc tccttcaacg
     8941 atcgatgcta agcaaagaga gaaaaatata atctccgaat tttgatgttc ggaatagcca
     9001 aggccagtca atacttgtcg actaatgtca gtgactgcaa gagaagccaa cagttcagca
     9061 tcttttacca gttccatgga tgtttttcca attcgaatgg ttgtcaaact caaataataa
     9121 gctaattcat ccattcgatt tggtgcgaat cgaagatctg atagtgaaat cgttttatct
     9181 agcaaatctc taactttggc gtaataatca atggaaaggg ttgcggttaa atacgtgagc
     9241 ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt
     9301 aatattgctt ctgaccctag cagcctaaaa ccagttttcc tagtatacga cagctgacaa
     9361 tcttccactt ctaattcatt tcttatcttt ttgatatctg acaggactgt ccctttagaa
     9421 acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc
     9481 aaataaagaa gatctcttcg ctcttgttcg gtatacacag cttcaaatcg agtaatatcc
     9541 aaaaattttt ctgcccagtc gattgctcgg aatttcttta tacggatacg ggtatcatcc
     9601 acatattcgg caaaaggagt taattgtttg atcgcctcgc ttgtatttac ttcgatgatc
     9661 tctgctgctt tttctagact gatttctctt ggtgttttca ggagataact aattttgtag
     9721 ttcacagcgc tgttcacctc ctacttatat tctagtatgg tagcgctttt aaatcgaggg
     9781 gtgtttggta caacttacag ttggattttt tttacgactg taccgaacga tttctatcga
     9841 atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc
     9901 cgatcaatga tgatcatgtt tattttttga ggtcagttct tcaaattttt ctttgacatc
     9961 ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc tcattaggct ttgtttcctc
    10021 ttctacagaa tttgaaccag ataaagctgt ttctttattt tctgtttcac cagagtttgt
    10081 gccagctaat gattcttctt tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac
    10141 ttcttcttct gttttagggg ccattgaacc tcttaatcgt ttgtgttttt caccgtcata
    10201 atttgtcatg ttggttcctc ctagtagttc tatacttttt catcgtacaa taagacagtt
    10261 tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca tatttttcct tctgattctt
    10321 gaatgctttt gattccttct tccaaaattt tcatttgtaa gattgtttct tcatctttga
    10381 tgactttttc tgcgccatta actaatgtct cgtaaatccc ttcgtagaca cggctgtaat
    10441 caccaacctc ggaaattact ttttcttcat gataactccc ttgttcatct atataggtca
    10501 atacaccata atgttcgggc aaatcgatac caaagtcttc atgatcaggc atgtaaaaca
    10561 ttttcagatg ttcttcttgt cgatcttttg tttgtttcac gaacatccct ttttttccgt
    10621 aaacgacgaa acttggacgt tctttgatgc ggaaataact ggattttatc gaaattttca
    10681 tatttccata gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc cctaaaagtt
    10741 gacgtacatc atagctgata tcatctggtt cgccgaaata agaaagaact tgatctactg
    10801 tatgacaagc atgtccatat aaatagctgt tcgcagtaga aaattcatgc gtattttctg
    10861 gtacttccgg tctgaaataa tcataatgca tttctacttc taaaatatcc cctaattttc
    10921 cgctttcgat tactttttgg actgtcaaga agtcagaatc gaaccgtctg ttttggtaac
    10981 attggataaa tagacctttt tcttttgcta atgcaaataa ctcttctgct tcctttgacg
    11041 tttctgcaaa aggtttctct actagtacat tcttaccatg ttcgagtgcc atttttccat
    11101 attcataatg aaattgacta ggtgtagtga tgacaactaa ttggatctca ggatcatccc
    11161 aaagatcaga cagctgatca gtatattcga tgtcttcgaa tgttttccac actggttcag
    11221 atggggaaaa aatttttttg atattgattt tgttcgtaag ttttcttgaa aaaggcaaat
    11281 gatagcgatt tgtactcttt ccattgccaa gataagcgat tgtaagcatg agtggtgcct
    11341 ccttttattg atgttttcag tatatcctag aaaagcgcag aaagtattta taatagctat
    11401 tctggtaaaa aagttcagaa aaacacttgt tatcattcgg tttggtgaaa ttaatgttca
    11461 aagtaaggat aaaaatcaaa aagatagtag cggaaccaag tgtttatcag taagatagaa
    11521 tttggaggtc agatcatgct tattgaagaa aaactaacaa aacaagagtt gtttaccacg
    11581 acagagaaac ggattgtcga ttacatccgt aggaatatcg aagctgcagt ctacatgacg
    11641 attgaagaat tagcaaaagc tacgtatact tcccattcag ctatcattcg tttatgcaaa
    11701 aaaatgggat tcagcggatt taaagaattc cgtttcgagc tggggcggga agttcatcaa
    11761 ttgattgctc agttcaatca aacgattgat cctaattttc ctttttcaag tgaagatagc
    11821 actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa aaaagcacaa
    11881 ttgcagattg aacatgaaga tttagatcaa atcggtaaag tattgaacaa ggcacagcga
    11941 atctttcttt ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca gaataaaatg
    12001 gtcaagttga ataagttttt gatcatggct gaagaataca gtgattcttc ttggaatgca
    12061 gccaatttga ctagcgagga ttgcgccatt tttattagct acagcggtcg catccatcat
    12121 tatgaacgaa taatgaccta tttgaagcat gtagaggccc ctaccttgct gattaccgga
    12181 aatcagcatt cagaaatggc caaacaggct gatatgtgtc ttgttatatc gcaggaggaa
    12241 tatgattttg ctaaagtagc gactttctcc tctcaaattg ccttcgatta cgtattaaat
    12301 acgttattct cagtgatata ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac
    12361 aaacaagaat tgatgcaaaa aggccttctt aaagaatatc ggaaaactta agaaaagatc
    12421 ctcatttagt aataagaaag atagtgtttg ataggctctc tttgatatca tcataaaaac
    12481 aggctgaagt tttctttttc aaacctcagc ctgtttgttt acggaacgat tcgaatagat
    12541 atattataga aatgaggaat agctaacttc tgtttcttat tttgaacgat tgacgaaaaa
    12601 gatatataag aaggaggaaa atcagtatac atgcagctaa caatccatac aggaaaaatc
    12661 gtttgttctc ggatactttc tgtacttctt tttgaacttc ttttgcatga taaggaaccc
    12721 ggtgccctct gacaagtaag cgatgactgt tgaccatata aggtgtacaa gttaggagtg
    12781 tcacgaaatc ctgccctttt tcgattctca gatcttttgt atcagtcggt tctaccgtct
    12841 tgatctgatc gacttgataa gcgagtgtcc gtccattgat ttcgatatag aaagaatcac
    12901 ctaaagtcag tttcggtaag tctgtgaaca actttgcttg aggcagtcct cgatggctgg
    12961 aaataacggc atgtgtattt tctccccccg taggataaga cgttccttcc aataatgagg
    13021 cacctttttc tagcagcaca gtattcgttt catcaaaaat tggcaaatgg acattgatct
    13081 cagggatcgt caaaataccg atcgtatgtt tctcaaaata ggttctatcc tttttcgcag
    13141 gttctttttt cttagtaaag ggatctgcac ctggatttcc accttctttt gctaattgct
    13201 ggtttttctt tgtcatgttt tcttggattt tagccatgac ttcttcattt tctttgaccg
    13261 cttgttgctg ataatgagag atgatctgct gatccagata attattcagc gcgtcgctaa
    13321 caaatggata agctagtatc cctattcctg agagaagcaa gaaaagcatc agcagatcta
    13381 gaagtttcct tttctttggt ttcatttgtg ttctccttgt gatgactgtt tgataataaa
    13441 agaaaaagga gcatttgcgt aatccgctgc tcctttttcc aattcttgca aaaagactaa
    13501 atctgactgt gcttgcgtct agcaaagtac agtccagcaa tcagtagaag gactactcct
    13561 gcaccgatat acacatagat tcccttaccg cctgttgaag gaagtgtacc tttgtgttta
    13621 tttggtattt tttcaggaga aatcaactgt cctgctgtta cataagattg ttcatcgata
    13681 gtaaatgcta cacggtttgt taatggaaca tatttttctg gcgctttcgt ttcttccaga
    13741 tagtacgtgc catattttag acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa
    13801 accgttgctg attctttcgc cgatacccag ctgacggctt ttgtggatgg gtcgatcgat
    13861 aaatatttcg ctgtgtcact atcttgatca cgaacgacga attctgctcc agcaagtgtt
    13921 tggtctgatg taacgtcacc atctacttta acaaatcgtt tgcccccagt aacgacatcg
    13981 actgacggtg gtgtttgatc atttgtatgc ccgttatcga catttgcttg gttagaaaat
    14041 cctttggttg gatctgcttt ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg
    14101 ccaggagtta atgaaggaat ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc
    14161 tcagtgacag aataatttac tgggtcgatt tctttatttc catcatataa ggcataagca
    14221 tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt catgagtatc gataagtttg
    14281 aatgttgtgt acttgttttg cgttccttct ttatcagcga tacctaatgg gatattgaca
    14341 gaaatctcat attgaatttt ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt
    14401 gttttatcaa ctttagacgt atcatttttg atggtctttt ctactggtgt ttggctattt
    14461 gccaggatct caaaaggcgt gattgtttgc ttttcaatca tttccgcatt atcaggagct
    14521 tttacttctt ctaaattata ttttccaact tctaaatgat taacgatcaa ctggcctttt
    14581 tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa
    14641 tgtttggctt tggtttgatc agttgtccaa gtgtacaatc catctgtgac actttggatg
    14701 tattttttga cgcttggtgt tccttcttct ttagaaataa taaattctgc tccatttagt
    14761 gcttcgtttt cggcagtacc gatttttgta actttcaacg ttccatcatt accgactgta
    14821 tttttagggt agagatggat agtatctagt tcttctgtcc cgtatttata agagccatct
    14881 gcttgtttga tcatctcata tacagggaaa gctaaaacca tgtttgcggc agctgacact
    14941 ccgtcttttg gttcttcttt gatcgtgtag actgcatctt tcccattttg ttttttaggt
    15001 aaagataaag tgacatttcc atcagcatct gtcgttcctg aagcaactgg tgtaccagga
    15061 gtcaaagact ggactgcttg ttttgctgca tccacggacg ctcctttatc tcgttgcgca
    15121 taaaattctt gagtgacgtt ataaactgaa aatgaaatat cggctaatcc ttggtattga
    15181 tcgaattcgc tcatttcttt cccgctgttt tggattaaag gatcgggtaa atcagtcatt
    15241 ttctttttat gaaggatgac ttgagctgct gtctcctctg caaacacttt ttttgcgccg
    15301 aagctgtttg tgagtaatgg taaaataagg aaaaggactc ctaacataac gtttattttt
    15361 ttatgatttt tcattttttc actcctgttc tttttttgat aaacaaccag attgcaaagc
    15421 ttgcgaatac cataccaaca acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa
    15481 gaaatggacg gctattttct tttttattga ctactttcaa tatactgtcc gcctctttat
    15541 cctcagaaag aaaagttgaa atggtaaacg gaatttctgt ttcacttaaa atataaccag
    15601 aaggtgcttc gatctctttt aatcgataac ttcctgtttt caacccggaa attgaaaaag
    15661 cgccattttt atcggaaatc aggtgaagcg catctgattc gttctttgtc cacttgtatc
    15721 cgttggctgt ttgctggagg taatttcctg ctttgttttt aataaggaat actgcgccag
    15781 ctaatcgttt atctggttta tttgtctcga ttttgacaaa ttgcttactc cctgtatata
    15841 cagagacttt ctttgtactg acaccaaaac caaaatccag tgttccgttg ttgttgattt
    15901 ccttgttcgc ctgtgctgta ctgcttagct gcatctgata agaaatggtc agtttttttc
    15961 cagcatattt ttccaacgaa gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt
    16021 gtatgacaaa gccttctgtc agcttttctc cattcgatga aatcgttaaa ctattaggta
    16081 aaaacgttaa tgcttcatcc gcactatctg acaattcgaa cttttgatag tcaaggatat
    16141 ttacaggaat tgtcgtatgc aaggaataag tgattttttc accaatcgta aaatcatttc
    16201 taggctcttc taataccttc tcaaaaggtg gatcagggta gtcgttttct tcatttttag
    16261 gatacaaatg gatagttgaa agtttttgat tgttttgtcc atgtacagga agaacaacta
    16321 ccatattctc tgccttttct ttgacgactt ctggtacttt ggattcaatg aaaagatacg
    16381 ctttatctct tttttcttga tctttagcgg ccagagaaaa agaagcgata ccatcttcgt
    16441 tgttaagagt tgtagttgtt gcttctgcgg taaacatacc ggaagacgca ccgttttttg
    16501 cgatctctgc ttgtgcttct tctaccgttt tgcccttttc ccgtagatgg taaaaagaat
    16561 ctgtgacatc ataaacttgg aatgtgacac catttaatcc tcgatacgtt tgtaataaag
    16621 ctttttcttg tccgtcattt ggatgatttt tcggcagttg cccgtttggg aaaagcaatt
    16681 tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt tgctacctga gtaaaagctg
    16741 gtttaaatag taacaagaag agacacatac taagccaacc aagttttttc atatttagct
    16801 gccccctctt tcttttttca aaaaaagata ccaaatagaa aacgcacaac caatcatccc
    16861 tactagatag attcctaaac ggccaattcc tcccgtttca ggtaatggta cttttgcctg
    16921 attcgtgatg tctaaagaaa tctggttgtt tttggctcct ggtgacagaa cagattcatg
    16981 atcttgtcca tccacttgaa ttgacccatc ttcgtgtata actatagtaa ctggctcttt
    17041 tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc gtataagttc caggttttaa
    17101 attttcgaat agaaaggtat ctgtttcttg tccatctttt ggcgattcta agtccatttc
    17161 tggtccctgc agtctgaatt ttgctccttt taatgtctgt ccgttgtctt cttttttgtg
    17221 taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg agcgtccata cattatttgt
    17281 catctcggta ttttttcccg tgtaaatgat ttttccatat ttatcgattt ggaattcata
    17341 atttcccggc gaagtatcat atccggcagg accagctgtt tcaacgactc gatatttacc
    17401 aggtgtgagt gaatcaaaac tggcaagacc tttttcattc gttgtttggc tgtcaattgg
    17461 ctgataagta ccatttgctt cttttttctg tagggaaaat gctgctcctt taagatttat
    17521 ctctttgtcc gtcccttgca tcgcatactt acgtactgct acaggaactt caacaaaagg
    17581 attttcaata gtcaactgga tcgtatcatc ggaagtagtg actgtttttc catcaatggt
    17641 taccgtacca tcagaagcga tcttgatttc ccaagtagtc tttttgctta atccatgccc
    17701 ttctggagct tttgtttctg tcagcgtata ggtcatttct ttttgcaatt tgacattttc
    17761 tggaagagaa taggttccgt cgccatgatc tgttagtaaa gtatcaatag aatctcctgt
    17821 taatttgaaa acagcgccaa taagatcctt ttcacctgta gaggaatttt tcgttatttt
    17881 caagtttaac ggtgtgaatt gtttagtatt tttccatgtc attgcatcta tttgttgaga
    17941 atcgtatcct ggtacaggta attctttgat tgtttggtaa ctgaatgctt gaccttgatt
    18001 attgtattga ggtagtgata atatctcttg ataactttct ccgctatttg cagataattt
    18061 gtcaatgtct gcacgttccc acgtatttgt tgtatcttta gctggtttaa tgattcgaat
    18121 atatccgttt ttccaagctg cagcatttgt cgtatgttcc cgttgaatct caaaagtaac
    18181 ttgatctgga cgatcagcta gattgttgtc aaattcttcc cataactttt tgatgtgaag
    18241 actgactccg ggagctttag cagatggtat accgaattca gctaattcat tggtatcaat
    18301 gtttggctgg aaagttgtcc tgccgttcat ttgataccag aaatttggat ggaagtcctc
    18361 attttctgtt tggattctca cttggtaatg gatttggatt tcttggtctt ttcctaaata
    18421 gatctgattg ctcttgatgg tatttccttc tatggaaata gatggagtga ccgttgtagg
    18481 acttgtcccc acactcttga ctgaaagtgt accaggctga taaatgaatg gttctgcgat
    18541 tggatcattt atttgtccat tgcttacagt tgctgagatc tgtacagctt ttttggctag
    18601 gtattcagag atatctgttg catgatcagc tgattcatag taaagatcgc ctttttcatc
    18661 tgatgaaacc atttgtcgca tacgagactc tacttctgct tttgagagac cagctgccgg
    18721 atcgctttga agttggatgc caagaccatg tatttcgatt cctcgttctt tgagtgccat
    18781 cgcttctccg atggttgcga taaacgtact gtcgattctt ctggatagat tgttttggtc
    18841 aggtgcatca tagattcttg agattagaga agtatttccc ggccgatctt gcgtattaga
    18901 aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt acccgctgta ctttatagga
    18961 aaatgttggt acaccatccg tcagcaaaac gatcactttt ttatgtccat ttggaacgga
    19021 tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga
    19081 aggtgtaatg gattttactt gatttttcac tgaatcaaat gaacccatct gtactgcacc
    19141 gttactgtag ctatatcctt cgcttgagta gccgacatat cccatattga ttttgtctgt
    19201 gataccgcta tctgctaaag tatcgacaaa acgatcgaca ccaatcttta cttcaccgat
    19261 ccgattattg tcgttcatac ttcctgacca gtctacgacc aatacgagat caagaggcgt
    19321 gatatctttt tgtacatttc cacgagcatt caaataaaca tcaaacaatc cgggtgtact
    19381 tgtttccttt gcatactttc gtatcgcata gtctgcttga ttaccggttc ctccatattc
    19441 gatatacgaa tgggtccgat cattaggatc tccattccaa gaatttatac catcccattg
    19501 atttgttccg tttttattgc cttgatgatc aagaacattt gtatttcctg taggttgcca
    19561 actggcttta ggataagtgc cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt
    19621 agatggcaca ctcacagctg gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt
    19681 ttcgttttct tttgatgatt cttcttctat ttcgtctggc tttggtactg acacatctgt
    19741 atctttctta ttttctgtct catcaataat gtcatattct ttattatcga cgatcagctt
    19801 gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa ctcagatcga tagtttgttt
    19861 agtgtttgat gagatttcca acgtaatttt cccgctattt acctgataag catagccatc
    19921 tccttctttt ctttccacat cacttaagta attttcgact agaccaattt ctttttctat
    19981 cttttcagca ctggtgttat tcgcatttag cttgatgttc agtgcttttt ttccagactg
    20041 atccgaaatg acttcagctg aaattgtttc taaaactgtt ggatcatctg tagtgtctgc
    20101 tattgcagat aatggcacaa agtttgataa actcaatatt atcagcatga aaataacttt
    20161 cagtttcttc cctgtggtcg tcatttttcc cctccttttt ctttttttct gttttgacgt
    20221 gaatagtgaa aattattaaa ataaacaggc gttacataat tttatttatt ttaatatgat
    20281 aattattata cgaagggcgc taaagggaat cacaccaatt ttcataccaa ttggtttcat
    20341 ttatgaagtt gattccttcg attaatgagc gtttcattga aagaaaaggg ttgagggaga
    20401 aaaacatatg atcaggttgt tggaattaaa ctatagaaag atggaaaaaa tcattcgaat
    20461 tctgtccatt tctgacaagt ggatgactaa aaaagaactt gccaaacgaa ttggtagttc
    20521 agaatctaca tttgtaaggt atatcgaaga gatcaaaatc cgctggggaa cggatgtaac
    20581 gattcaatct tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac cagcttattt
    20641 aaatgttctg atggaaatgt cgcaaagttc tacaaacagt cgtttattaa atgagattat
    20701 tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca gccggtcttc
    20761 ttttacacgt aagttgaaac aatgcaatca aattttagaa agctatttat taaaagtgat
    20821 cgttgaccaa ggttttcagt taactagcct taaaagtgag ctccagttgc gtatctttgt
    20881 tacctttttc tttttaagtt actacggaag acgcacgttg ccatttccaa tcgaaaaaaa
    20941 gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg aatttgtttc aggaagaaag
    21001 cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga gagaatccca
    21061 aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa
    21121 aaaagacttc ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa
    21181 agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga
    21241 aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa
    21301 agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat
    21361 tttcccatac gatacgactc atttgacgat ccgtgcaact tactttgcac agcagatgca
    21421 acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct
    21481 actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa
    21541 tcctgaaatt caccattata cttctgataa aaaaatactg attatcagcg acttaagtct
    21601 tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca
    21661 ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga
    21721 tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga
    21781 tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa
    21841 aaaacagcta tgtataaacc accctgataa aaatttcagg acaaatacac atagctgttt
    21901 ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact
    21961 cttcttttgt ccaattattt gcaatgagat ccgtcaaagt gttcattacg agggtcatgg
    22021 aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta
    22081 ataattccag ccatttttcg ttcattggag aatcagccga tagttctcta cgtttggttg
    22141 tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat
    22201 ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt
    22261 cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca
    22321 aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccccatct
    22381 tgtcaacgat ttctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta
    22441 ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca
    22501 tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt
    22561 cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca
    22621 ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg tttctcatga
    22681 gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt
    22741 ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc
    22801 tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa
    22861 gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact
    22921 agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat
    22981 cctatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagtgagtga
    23041 ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa
    23101 gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa
    23161 aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact
    23221 tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta
    23281 ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc
    23341 cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca
    23401 gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg
    23461 actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc
    23521 caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa
    23581 ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag
    23641 gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt
    23701 tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag
    23761 caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg
    23821 gagaaactgt gtaataagtt tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac
    23881 tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga
    23941 tgatttcatt ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt
    24001 ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt
    24061 acaatccgat tggtgctttc tctcctcctt caatgtattt cagatctcct cttgttaatt
    24121 gtgaagctgc aaatacttga tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat
    24181 catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata
    24241 cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc
    24301 gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca
    24361 aaatgtcact tgttgaatag tgtaactcta aagtgacaat ttgtcacttg tgttgttttt
    24421 ttcttatcaa taaattgtct aagacatctt tcgataaaac cttaacatga gtcataaaaa
    24481 atttatttaa gccttattta tgctttccat gataaactaa agtgaccaaa tcaatgaggt
    24541 gggaaaatga ttaaactatt agtagtagaa gacgataaag tattatccaa taatataaaa
    24601 gaaatactcg ctgcaatcgg cgagataaca caagtttatg atggtagtga aggactgttc
    24661 gagttggaaa gtggagttta tgatcttgca atcttggatt tgatgatgcc ggaaatgaat
    24721 ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac agactccagt attgattttg
    24781 actgcaaaag atagtttgga agataagatt gaaggatttg aacgaggtgc agatgattat
    24841 ctaacaaaac ctttctatcg agaagaactg atcatgcgcg tgaaagcatt attgaaacgc
    24901 tcgctaggtc tctttgatga aaatgttctg aagtataagg atatcagttg tgatttaaaa
    24961 agcaatgaag tcttgtataa agaacaagtt ttgcccatac aaggaaaaga gtttgaattg
    25021 ctcgtttatt ttttacaaaa taagggaatc attttgacaa aagagcagat atttgaccgg
    25081 atctggggat ttgattctga tacgacgatt acagttattg aagtttatat gagcaactta
    25141 cgaaaacatc tgcgaagtac tggattggat aaagaaatca aaaccttacg taatgtcggt
    25201 tatatactgc aaagggtgtg atgcaatgaa tggagatatt acacgaaagc aacgtagtcg
    25261 cttttttgcg acaaatgtag cagcatttgc ttttatcttt tttcttttgg gattcattac
    25321 tttacagata ttaaaccaat ctgcatacag agaaacagat ctgtcattag agaaaataaa
    25381 ttttgattct cgaacagtac aattagagat acaaagatat cagcaaggcg atcccttttt
    25441 aaacccgact caaacggaac aaaacagaac aaacatggaa cagccattaa tggaaccatc
    25501 aaatgaccgt tttaacactc aagtgatctt gtggtctaaa aatggtgaga tattgaataa
    25561 agcagctatt ggtgggcgtt tcaaccaaat caaagaatta aaaatggata cgaaaaatct
    25621 gaataccatc caaaatattg aattaaatga agacagtggc tcatctgccc tctctttccg
    25681 ttcgattacg aaaaaagcgg agacgaatca agatggcgtt gcctacatcc aaattttaga
    25741 aaatacaaat caaatcaaga attcattagc gacttttcgc acgattatga ttttgtgtat
    25801 gattgttttt tggattctct ctattggtat cagttattac ttgtctagtc taagcatgcg
    25861 ccctattatt ctttcttgga gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt
    25921 gcgcacaccg ttaacgatta ttcaaaatag tttggaacat ctttttacca agcctaatca
    25981 tactattatt gaagaatccg aaagcatcgc tcaagcattg tcagagacga ggcgcctcac
    26041 tggattgact tcggatttgc tgacaatcgc tcggaatgat tctgatcagc agctgctttc
    26101 aaagcaaatg atcaatacac aagaatatat cgaacaactt gttaaaccat ttcaagagat
    26161 ggccatcata gatggaaaag agtttattct ccataacgat gcaacattag aagtaccaat
    26221 tgatgaaaaa aaaatccatc aagttttggt tattttgctg gataatgcat taaaatatac
    26281 gaatccagaa gataagatca ttttggattc atctgttaac cataaagaat ggctgattca
    26341 cgtaaagaat actggccctt ctatcagtga agaagatcaa gcaaggattt ttgatcgatt
    26401 ttatcgggaa gatcaatctc gggcgaaaga gactggcgga tacgggcttg gcttggcaat
    26461 tgcaaaacag gtaatcgaac agcatcatgg acagatttct gtatccaatt atcttccaca
    26521 aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa aaataatgct tatccctaaa
    26581 aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca
    26641 gaagtagctt cttcggaaat aagccgaaat ttctttaagc tgaccacttc tgtcacaacc
    26701 tctttagctt acgcttacga ttttttattg cggtcttcaa tgtgtacatc accgcttgtg
    26761 tcaatatcca attcttctcg gctaacatct tctgtcactt tttcaacatc ttcatgttct
    26821 tctttatgaa tcccaacttc ttcacgaata actgtatgtt tgcttacatc tacttgctct
    26881 tctttgattg ggatagtaat cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat
    26941 tgatctgtta ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg tacttctacc
    27001 gtttcagtat cgttgaccac atgtttgtgg atgtcgactt ctccagttgt cacatcatgt
    27061 gtatttacat ctaatttttc ttcttttagt tgaattgtat catcatctag atttggtttg
    27121 cgatcgatgt cttcagtctt gcttgtaccc attgtatcag tctcgccagg tacgtccatc
    27181 ggatcagttc ctggtgtcat attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca
    27241 cctacgtcca ttggatctgt tccagtgcca gtttctaatc cagtccccat tgtgcttgct
    27301 gggctaacag tatcttgtgt atccattgta tcttctgttg tttctggctg acgatagcct
    27361 tttactgtga tgacaaattt tccatcagaa atatctcctc tatattgata taaaggatca
    27421 ttttctggtg tagccgtctc agaatcatag gtatcaaaag aaaaagtatc ttttattttt
    27481 tcccataaag aacggtcttc ttcaccatcc atccgatttt catctgtttc aacatctata
    27541 ccagacaatc cgtcaaatcc tagtctagat gctgcttcat cagtagtata aataacgata
    27601 tcatctcgtt catacccttc gttacgcaac cgttcaatta ctgataatgc ttcttccctc
    27661 gtactatagc ttccgatgac cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa
    27721 tagatttatg attggtatat tgttact
//
LOCUS       DACCVU010000028        27241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCVU010000028 DACCVU010000000
VERSION     DACCVU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.028"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            76..393
                     /locus_tag="IVW29_001045"
     CDS             76..393
                     /locus_tag="IVW29_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAP7838544.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            390..551
                     /locus_tag="IVW29_001046"
     CDS             390..551
                     /locus_tag="IVW29_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7838545.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            548..853
                     /locus_tag="IVW29_001047"
     CDS             548..853
                     /locus_tag="IVW29_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838546.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            853..1167
                     /locus_tag="IVW29_001048"
     CDS             853..1167
                     /locus_tag="IVW29_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838547.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1160..1690
                     /locus_tag="IVW29_001049"
     CDS             1160..1690
                     /locus_tag="IVW29_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7838548.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1687..1956
                     /locus_tag="IVW29_001050"
     CDS             1687..1956
                     /locus_tag="IVW29_001050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838549.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            2086..2373
                     /locus_tag="IVW29_001051"
     CDS             2086..2373
                     /locus_tag="IVW29_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838550.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            2860..3273
                     /locus_tag="IVW29_001052"
     CDS             2860..3273
                     /locus_tag="IVW29_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7838551.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            4469..5014
                     /locus_tag="IVW29_001053"
     CDS             4469..5014
                     /locus_tag="IVW29_001053"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7838552.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            5015..5332
                     /locus_tag="IVW29_001054"
     CDS             5015..5332
                     /locus_tag="IVW29_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7838553.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(5489..5653)
                     /locus_tag="IVW29_001055"
     CDS             complement(5489..5653)
                     /locus_tag="IVW29_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAP7838554.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            5755..6276
                     /locus_tag="IVW29_001056"
     CDS             5755..6276
                     /locus_tag="IVW29_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7838555.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            6248..6703
                     /locus_tag="IVW29_001057"
                     /pseudo
     CDS             6248..6703
                     /locus_tag="IVW29_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(6743..6901)
                     /locus_tag="IVW29_001058"
     CDS             complement(6743..6901)
                     /locus_tag="IVW29_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAP7838556.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            6994..7755
                     /locus_tag="IVW29_001059"
     CDS             6994..7755
                     /locus_tag="IVW29_001059"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7838557.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            7942..9294
                     /locus_tag="IVW29_001060"
                     /pseudo
     CDS             7942..9294
                     /locus_tag="IVW29_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            9310..10530
                     /locus_tag="IVW29_001061"
     CDS             9310..10530
                     /locus_tag="IVW29_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7838558.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            10565..11242
                     /locus_tag="IVW29_001062"
     CDS             10565..11242
                     /locus_tag="IVW29_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7838559.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            11239..12432
                     /locus_tag="IVW29_001063"
     CDS             11239..12432
                     /locus_tag="IVW29_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7838560.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            12447..12773
                     /locus_tag="IVW29_001064"
     CDS             12447..12773
                     /locus_tag="IVW29_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7838561.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            12777..13112
                     /locus_tag="IVW29_001065"
     CDS             12777..13112
                     /locus_tag="IVW29_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7838562.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            13109..13504
                     /locus_tag="IVW29_001066"
     CDS             13109..13504
                     /locus_tag="IVW29_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838563.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            13501..13872
                     /locus_tag="IVW29_001067"
     CDS             13501..13872
                     /locus_tag="IVW29_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838564.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            13888..14448
                     /locus_tag="IVW29_001068"
     CDS             13888..14448
                     /locus_tag="IVW29_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7838565.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            14573..14884
                     /locus_tag="IVW29_001069"
     CDS             14573..14884
                     /locus_tag="IVW29_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838566.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            14908..15099
                     /locus_tag="IVW29_001070"
     CDS             14908..15099
                     /locus_tag="IVW29_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838567.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            15099..18947
                     /locus_tag="IVW29_001071"
     CDS             15099..18947
                     /locus_tag="IVW29_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP7838568.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            18948..19820
                     /locus_tag="IVW29_001072"
     CDS             18948..19820
                     /locus_tag="IVW29_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838569.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            19817..20866
                     /locus_tag="IVW29_001073"
     CDS             19817..20866
                     /locus_tag="IVW29_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838570.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            20870..21694
                     /locus_tag="IVW29_001074"
     CDS             20870..21694
                     /locus_tag="IVW29_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838571.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21712..23772
                     /locus_tag="IVW29_001075"
     CDS             21712..23772
                     /locus_tag="IVW29_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP7838572.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            23935..24381
                     /locus_tag="IVW29_001076"
     CDS             23935..24381
                     /locus_tag="IVW29_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838573.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24383..24520
                     /locus_tag="IVW29_001077"
     CDS             24383..24520
                     /locus_tag="IVW29_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7838574.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            24555..24797
                     /locus_tag="IVW29_001078"
     CDS             24555..24797
                     /locus_tag="IVW29_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7838575.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            24813..25010
                     /locus_tag="IVW29_001079"
     CDS             24813..25010
                     /locus_tag="IVW29_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7838576.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            25021..26037
                     /locus_tag="IVW29_001080"
     CDS             25021..26037
                     /locus_tag="IVW29_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7838577.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            26152..>27241
                     /locus_tag="IVW29_001081"
     CDS             26152..>27241
                     /locus_tag="IVW29_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838578.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPID"
BASE COUNT         9556 a         4529 c         6000 g         7156 t
ORIGIN      
        1 ctgaccctcc aaactctcca ccaaaatctt cctaaatgct ttgataccaa cgattaaaca
       61 gtctggaggg ttacattgaa atcagaacat caaatacaag acgagatcag aattgcctta
      121 tcaaaacatg gttgcacgat ttttcgatcc aatgcgggac aagtgacaac gatcgaaggg
      181 cggcaatttt acgggatgcc aaaagggttc ccagatctat gtggacatcg taattctgat
      241 gggaaagcaa tttatataga agtaaaaaat gaacgcggta agttgcgtga agatcaaaaa
      301 aggtttgctg aatttttaaa cggtcaacct gtactatacg gtgttgcacg atcagtggaa
      361 gaagcattga aaattgtgga ggaatcatca tgacaacaga agaagtgatt caaatgcgta
      421 ttcgaagcct tcagcgtgag attgacgatc tggaacggac aaaggcagtg atggtcaatg
      481 aaacggcgag gaaggcaatc gatttgcaca tagagaattt aagaagggaa atccatcgat
      541 tggaggaatg agcgtggata agaaagcagc aatgaaacga atcatcgaac tgacacattc
      601 tgagaattgg caagaagaca aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat
      661 gtggagtgaa aaaaccaaac ggagaacacc gagaaaaatt gcaatctggc atggtgaccg
      721 aattctagta acaggtactg ctgaacagtt atctgaaatt actggattaa gcaaaaacat
      781 tatctgggat agagctaaac gcgagaacgt tgattcgaaa ggtcgtcaat ttaaacattg
      841 ggagaagaaa taatggagga actaatcaca aaagtagagc agtgggcaaa agataaggga
      901 ttggatcaag ctgattccag caagcaaatg ttaaaaacga tcgaagagat tggggaagtt
      961 gccgcttctc tagctagaaa agatgaatat ggtttaagag acggaattgg agacgtagta
     1021 gtaaccttga ttattttagc tatgcaaaat gatatggatt tgtacgagtg tctgaaccaa
     1081 gcatacaacg aaatcaaagg acgcacagga aaaatggtag atggtgtatt cgtgaagtcg
     1141 agtgatttgg aggacagcga tgaataaaaa agtattaatt gataaacaag cattgattga
     1201 tgaattaatg aaaatacctg gtgtgggatc taatagtgac gctttagaaa cgattaaacg
     1261 tttcccatcg tatgaaccgc agaagccagt tgtgcctaag tttgtggcgg aactactcga
     1321 ctattatcgt cagtcaacag atgttgattt attagcttta ttgataactt tccatgattg
     1381 gtattatcgc aaaactaaag acggtgagca tgaagaagca attgattggc tagttgatca
     1441 tcctgaaatt tatatgcgtg catggctgtt tggctacgag ctcgagaaag agccgttgta
     1501 ttgcgtaata atcaatggta gatatcttgt caaagtattc agcaatacaa acgttgtcat
     1561 attagttcca gcagatgaat tttcacagta cgttactcaa aaatttcaat taactgaaaa
     1621 acaaatcaaa gcaattgatg aaagatactg gccgtttgct gtgccagtgg atgaggtggt
     1681 agaaggatga gcgacccata cattactaaa ttagaaaagt tgtccgaaga ttttgagaag
     1741 aataaaaata aactaactaa agataaactt atagaactgg ttaggttggt tatcagagga
     1801 gctattacaa gagaaaaggc gcagcaaaac atagaaattt ttgctcgtag tgtccgagat
     1861 ggattagaga agaaagttaa acaaaatgaa caacagacac cgcagaataa caaaactaag
     1921 aaaacgggaa ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga
     1981 agaaacatat aaagtggcaa gtcagtgtgt tgctgatgcg agcggcgcta ttcgtaagtt
     2041 tggaatttcg atattaagtg atgatcgtaa atgggaggaa aaagaatgaa actaaaagac
     2101 ggattttact ccagcagtca tggtatcggc ggtttaatgc tagatatgcc gacaaagaac
     2161 cctaaaacac gtgagaaacc aaaattcaaa gtcggtgaca tggttcgctg tgaagcagaa
     2221 ggattcatct atccattttg tggatatgta gaacatctct ataatcactc agcaatcatt
     2281 cgtattgaaa acacgatgga atgtgacaag tggttagcga aaagcaaaga gaatttagca
     2341 gtagcgagat tggtggatat ggaggttata taaacagcga aaaaatgtac ttctaagaga
     2401 aaaatttatt ttttataaaa ataggaaagt atcattatga aaaagaccac agagataaat
     2461 aaacctacca cataaaaaat gaatatttct tgcgattcaa tataggtata tttaacaatt
     2521 tgtacaataa aaaatactat aggtaaaaaa gataacatga ggagcttatt tgttctagag
     2581 tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa cgggaatcca aaataacagc
     2641 atgctccaca tagtcactca accatctcct atacgcttta ttatcaatag ataatatcat
     2701 ttttcgtaat aaagataaaa aaataagtaa ggcttctcat taaaatataa aaaagacagc
     2761 cgaccactga ctgcctatat aagagtattg aaataaaaag ctgctgatat aataaattcc
     2821 acaagtttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta
     2881 gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag
     2941 cggatggcag gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag
     3001 gcgccgaagc acggcaataa ggcagaagac gcaatcattc agatgatgga tatagaagcg
     3061 gagagagacg cgattctagc agccttgatg gctcttagtc tgattagtcg tcagatactc
     3121 tactatagct tttgtgtgcc agatagcttc tcaaactaca gaattagccg tgaagtgggt
     3181 tattcagaaa gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca
     3241 tataaacacg gaagaataat tgcttataaa taatttggcg gctttttggc ggaatgatgg
     3301 cggtttttag ctatttacca gtgatattat ggtagtgtcg aaagattagt gataggtcta
     3361 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
     3421 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
     3481 attatctatc atccgttgct gtctattgtt tttttaatta ttcacacgat aactaaaggt
     3541 gagcgaagag aaatgattcc attaataatt tcaatttttg cgctttgtct taatgtctat
     3601 atgattggat ttaaaaatgg gcaaaataaa agatagtagt aactaagaaa gaaattttta
     3661 tattgtcact gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc
     3721 aactatgcaa tgttcgattc attgccagtg actttggcaa ccgaggcatc ggcggtttaa
     3781 aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata
     3841 ttagatcact cgttgagtgg tctttttatt ttacataaag gaggtaacaa cgatgtatag
     3901 accacaatac ttagaacaga agtatgaagt aatcactgtg caaaacggta acggtgagat
     3961 agtacgaaag tatagaagac caataaagag cgatacatat aaacgaaagg aaaacaatga
     4021 agttatttct tttcgcagaa ggagaaaagc caaatgagaa actactggta tgtatcgcta
     4081 actaatgaat atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa
     4141 atcaaaggga agtactctat tgtcgaaatg ctaagagaag ctacaccaaa cgaagtggat
     4201 aaatgcaagc tgatatattg cggtcatggc tattggaaag acgagtatat ccaacaaaac
     4261 attgagaggt ggatagatag atgagttacc tcgaacattt gaaacgttgc tacatgcatt
     4321 ctaagaataa acttcctgac agctacactg tagatgatgt tgctatacat gtattgaaga
     4381 ctgagagcca tagcagtcct gatggtagca gtaaggagca gacgctcgcg tggttcaagt
     4441 tctttaagtg gattaaggag gaagagtaat gcacatgcac ataggagaaa agatatataa
     4501 attgagacgg cttaataacg aaacaatgaa cgacttagtt aaacaaactg gagtgtcaaa
     4561 atcatggtta tctgatattg agtctggaaa aaagcaacgc gttgatttta ataagatatg
     4621 tgcaatagcc aaacattata aagttagctt agaatacttt cgggatgatt atttggatag
     4681 tgatgaacac gaggatatta ttcctttgac aggcgaaaca agagttaaat cagtgaaatc
     4741 gcttgaacaa agcctaaagt atctgaataa caaacaagaa cctaaccttc tagagatcaa
     4801 actaaaagat actgactcag taccagaggt ttggtacaag ggtgagaggt tggatgaatt
     4861 gcctaaagga ttagtagatg tctcgtatca ttggaagact gatgatttta ctaatgatga
     4921 tagaggagcg aacgacatca cgattcaata cttttctagc tttaatgata agtatccaga
     4981 taagaaaaca atcggacaca agagagatat gtaaatgaaa gaagttagac ctagagacga
     5041 aatagacaaa ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag
     5101 ggacttcggc atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat
     5161 ccatcacata gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga
     5221 atgtatctgt gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa
     5281 accaaaacct aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag
     5341 cgaaatgaag gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt
     5401 gctgtccttc cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc
     5461 gaccaattta agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag
     5521 aagtaggggg taatcggtcg cctggatcaa tttttacttc ttgtccgcct tggacatttc
     5581 caccacgagg acccacttct ttgtaggttc cttttggttt gttgtcttcg ccgggtttat
     5641 agagttctcc cataggaatc cctccttaac aaatttcggc acagcactgc cgataaccta
     5701 attataagga ttgtgataac gatttcaatc tatcttttga aagaaggtga tattatgccg
     5761 caaccagcga agagtgcaaa attacaatta ttaaacggaa acccaaataa gaagaatacc
     5821 gaagaactcc gcaagcgagc ggccgcagaa gacaaattaa aaatggctac tgacaaaatc
     5881 aaaccgccgt tatggctaga ttcgctagga aaggatacct ttgagtttat cgccgatgaa
     5941 ttgctgtctg tggatttaat cagtaatccg gacgtccata caatggctct atactccaat
     6001 tggtattcgc aatacgtttc tttagaaaaa cagcttcgaa aactacaacg agagtacaag
     6061 ttgaactatg cgcttgcgaa aaaggaggca gaggcgagag gtgagccgtt taatgaacct
     6121 aatgaattaa ttggtaatcc gctctctcgg cagatggata cagcgtctcg gaatctccgt
     6181 tcttttggcg ctgatttagg actatcacca gcagccagag ctaagttagc tattaagatg
     6241 gctgatgatg gtggtgatga cgatgacgac ttctaatatt ttggatatgt cttacacaga
     6301 acgtgtggac tattggcaaa gctatcttga ggagcaagct tcttggggtg gctttttaaa
     6361 atgtccatat ccggaattgt taactacttg gtatgcggaa cgattaatcg atggaagcat
     6421 accagccagc aaagaaaata ttcaagctgc taaacggcat atgcgtgatt tgcagcgcca
     6481 aggaacagat gattttcctt ggatctttga cgaagaaaaa ggtcaccggc ctattagata
     6541 tatcgaaaaa aaatgtaaac caactgaagg cgactttggt tcgtttgttt tgcaaccttg
     6601 gcagcatttc ataattggat ccatgtacgg atgggtacat cgtgatacag gagagcgtcg
     6661 cttccgcgag gctcttattt ttgttggacg taaaaacggt aagttttgcc gtttaaaaat
     6721 cgggcaaaat cggtgaaaac ctttattttt ggctttcttt tagtaataat tcgatagctt
     6781 tatcaagcaa tttagacata ggaaccatag tttcttccga catttttttt aatccttcgt
     6841 ataaatcttt atcaattgcg ttagaaattc taattctgtt ttttagtccg tatttgtcca
     6901 tgtgtgacac tcctttttat tttactataa catccgattc caccgcttgc aactaccatc
     6961 aattgatggt agtatataaa agaggtgagt agattgaaaa aatacattat ttacaaacac
     7021 ttaaataaaa taaatggcaa aatttatatc ggtgtcacaa atgatattgg cagaaggtgg
     7081 aggagcggtg ggattgaata taaaccacca aagaacgaaa cccaacacca cagaagtttt
     7141 tggaatgcaa tacaaaaata cgggtgggaa aattttgatc atttgattat tgaagaagac
     7201 ttaacgatga aagaagcttt tgagaaagaa aagttttata ttgaacttta tgactcaact
     7261 aataagaaaa aaggttataa catcgcaaaa ggcggaaatg gcggaataat atataaagtt
     7321 caccccaaag gaatgctagg gaaaaaacaa tctaaagaat tttcttcaaa tcacagtaaa
     7381 tgggcaaaga accataagaa taattgtatg acaaacggtg atgttgtatg gggtgttact
     7441 cacgaacacc caaaaggcat gttgggaaag caccacagca aagaatcaat tatgaaaaag
     7501 aaagcatata gtggtgaaaa tgctgtaacc tcaaaaccaa ttgtcgcaat tgaacgggat
     7561 ggaactaaaa gagaatttca cagcgctaag ctttgcatgg cttactattc gattagtaca
     7621 tgtgtttttt accgactttt aaaagatggc gccccttatg tgataaatcc taaagcgaac
     7681 tacagaaata aagaaaaaat attagctatc gaaggaatta tgttcaaaca taaagaagat
     7741 accgaggtaa gttagtagat tgcgaaaggt tactaactac cgtaacgcgt aggagttgaa
     7801 taaatataat gctcccaaga gtgtccgaca gcagataatt tgctgaaaat gtacgctaaa
     7861 ctgggccaga actgactgac cgatgaaaat gagggtgacc tccagagtgc gagataaaaa
     7921 gctcgcagat aataacaatc gaaaacatca ctaatttcag gcctttccac atacatggtc
     7981 gcttatgatg atgaacaagg cgccaacgtt tacgtattgg caaatgctcg tgatcaagca
     8041 agcttgttgt ttgataaggc cgcagaaatg gtcaaacaat cgccggcgct ctttaagaaa
     8101 tttggtaagc ctaaacgatc aagtattaat tatgctcccg ccttttctaa aatggaacca
     8161 cgcgcctcag atagccggaa attggatggg ctaaacactc actttggtat ttttgacgag
     8221 atccacgagt ttacgaatta caatctgatc aacgttatca agaaatcaag aggaaccaga
     8281 aaacagcctc tgatagttta tatcacaact gctggatatg tattagatgg tccgttgatg
     8341 tcttattttg agcaaggtgt ggactgtttg gaacatttgg aagatgacat cgatgaacgg
     8401 actttctatt atctggcaaa acttgacagt gcggaagagg ctgatgaccc aagattatgg
     8461 atcaaagcca atccgaatat ttgtctaatg aattttgttg gcatgctaga tgactatgtt
     8521 aaggataaaa aagatccaaa agaatatgct gactggatta ccaagcaatt taacttgttt
     8581 tccgatatcg atgagctgtc atttgtcgat atgcctacca ttaaacgaaa caataaaacc
     8641 atcgatattg aaacgctcaa aggtaagaag tgtgtcggtg gttttgactt gtccgaaacg
     8701 gaagacttta ccgcagccgt tttagaattt ccgcttgaaa caggcgaggt attcattttg
     8761 caacacacat ggatcccaca agctagattt gatcgagata acaatcaaga gcgtatcaaa
     8821 gcgtgggaga aggtgggaga tctaacgatt attcctggtg attacgtcaa ttatgaatac
     8881 gtcttaaatt ggtttgtaga aaattcgaaa atctatgaca ttgtaaaaat caattatgac
     8941 aaggccaagg cgctacgatt aaataaagaa ctagaaaatg caggatttga aaccaacgag
     9001 attcggcaag ggtttctatc attaggtggg ccaatgcaaa acttcaagga aatgctattg
     9061 gacggtaagg tgattttcaa caattccaag ctttaccgat ggtatctatc caacgtcaag
     9121 ctggtgatgg atcgcaactc aaactggatg ccgtctaagc agtccaagag tagaaaaata
     9181 gatggttttg cagcaagttt gaacagccac gccgaagtgt tgaatatgtt ggttaatcct
     9241 gtcggaaccg ggaaagtaac ctattactcg atttccgatt taatgaatat gtaagaaagg
     9301 tgtggaggaa tgagtatttt agatcgtttg cgttcttttg gccgagcgaa gccgaaagcg
     9361 agcaaacaag agtatttttt gaatgacccg ggattgatac cgtatttagt cggaaaagat
     9421 gaaatatcag aagggatttt ttccgtaatt agccgtgtat cgaacgtttt tgcgtctctc
     9481 cctctcaaaa tgatagatgt ggagtttggc caaccggacg actgtcctgc atacaacttg
     9541 ttgagcgaag gccctcgata ttttacaaag tttgattttt tccgggacgt ggaagttttg
     9601 agaaactacc aagggaatgc gtatgtgcag attttccgaa atatcaatgg agaagtagca
     9661 gatatggcgt tagtaaaacc tggtgcttgc catccagtga ttgatatgga tagcggggag
     9721 ctttactacc aagtaactgc gactgacaaa ggcagttaca agcaagttat ctatgtacat
     9781 tacatggaaa tgcttcactt taaacaaccg aggtttggcg gcttggaagg tacagacccc
     9841 acaaaagtat taacgaatac cctcggatat gatcgagaag tccgaaaaat ctctttaagt
     9901 cagcttaaag gaagtaatga agggctaaaa gttaagtttg ctagcaatat ggatgaagaa
     9961 gctaaaaaag ctacagttaa aaacattgct gatttttatc gacaaaacgg tggactactt
    10021 gtggaagaaa acggtgtaga aatcgaacgt ttacaacgag agctggtaga cagcaagctt
    10081 ttagatactg ataaagtatc tcgctccaga atcgctatgg tctacaatgt gccggagcat
    10141 ttcatcggga ataaccagtc gagttactcc tctcaggaac agctcaatat ggaatttttg
    10201 acgtacaatc tagtaccgac cgttaatcaa tatgaagcgg aactaaataa gaaaacacta
    10261 tcgagaaccg aaaaagcaaa aggttatcga tataagttta atgtcgcaag tttgctaaga
    10321 gctgacactc aggcaagggg acaattctat caaatcatgc gacgaggcgg agcatactca
    10381 gcgaatgatg tccgaagatt tgaggacttg caaccgataa ataaaaccgg tatggatgat
    10441 taccatattt ccggagacct atatccaatc gatatggatc caacattaag aaaaacaacc
    10501 tcgtctaaaa gcgtagccga aaacggttag gtttttttag tttgcaccga agggaggtgg
    10561 aaaaatgaaa aaagtgacgt taagcggcga tgtcgtggat aacgataccg cgtggcttta
    10621 tgactggttt gggatcaatt gtatctcacc agggaaaatt tctgccgctc ttacagaagc
    10681 agcgggggat gaagtagaac ttgatatctc atcgaacggt ggggatgtcc tagcggcaag
    10741 cgaaatatat accgctatcc gtgcctatcc gggaaaggta tccggaaatg ttgtgagtat
    10801 tgcagcaagc gctgcaagtg taatcgcttg cgcttgcgaa ccgcttagaa tctcacctac
    10861 ggcacacatc atgattcata acgcatgggt gaccactagt ggcaacgctg aggaattaaa
    10921 agccaatgca gaaatgttaa gcagtgtgga tgagtctatt gttaatgctt acgagatcaa
    10981 aacaggactc gatcggaaaa aacttgctga tttaatggcg aaagatactt ggttaaatgc
    11041 tcaaacagca gtagcagaag ggtttgcaga tgaaattatg tttgcagaag caccagtaac
    11101 ggtactcaat gcctctcaac cggttattcc aaaaaacgca gtaactaagt tgaaaaattt
    11161 aatactcaaa gcggaagcac cgcaagaaga aacactctta cagaaaaaac taaaagcctt
    11221 aaatggaggg aaaaacgaat gaatttagaa caattaaaaa atgcgtgggt cgaggcggga
    11281 agtaaagtct ctgacttaaa tgcacaactc aatgcagcat tggttgacga tgaaaaaaca
    11341 gaagaagatg tagtaagttt gcaagcacaa gtaaaagcag cacgggctaa acgggacgga
    11401 ttgaaagagc aagtggcaaa tatggaagcc gaacaagtct taaacgtcaa aaaagaacca
    11461 ttagataaaa aagatgaaaa cttgaaaaac aagtttatca aagactttaa agcgatggtc
    11521 aatggtgatc ctgctattat ggctactttg acatctaata cggatgaatc tggtaatgct
    11581 atcggattga ctattcctgt agatgtgcaa acgactattc atactttggt tcgtcggttt
    11641 gactctttgc aagaatacgt aaacgttgaa aaagtgacca ctaccagcgg ttctcgcgtt
    11701 tatgaaaaat ggtctgatat tacaccgttg actgctttgg atactgaaga cggtgaaatc
    11761 ccagcaaatg atgatcctgc actttacttg atcaaatact tgatcaaacg ctatgcaggt
    11821 atttccacag taactaacag cttgctaaaa gatactgccg aaaacatttt ggcatggttg
    11881 tctaaatgga tcgcgaaaaa agtagttgtt actcgcaata caaaaatctt ggcagctatt
    11941 gatggaatca aagcggcaca aaagaaagat gttacagatg ttgatggaat taaagatatc
    12001 gtaaacgtcc aacttgatcc agctatcgaa gctacatcta tgtttattac aaaccaagat
    12061 ggcttcaatg ttttagataa agtgaaacgt gctgatggat cttacttgtt acaaaaagac
    12121 gtagcttctg caactggata taccttctta ggtaaaccaa tcaagaaaat tgcttctcgt
    12181 ttcttgccaa acaaaggtac gcaagctact cctaaatatc cactgtacat tggtgatctg
    12241 aaagaagccg ttacattgta tgatcgcgaa aacatgagct tgctgacaac gaatattggt
    12301 ggtggagctt ttgaaacaga caccactaaa gtacgcgtca tcgatcgctt cgatgtgcaa
    12361 ctagttgatg atgaagcggt tgttttggct acttttacaa ctattgcgaa cgagacaccg
    12421 gcgaaagttt aaggagctga ttccttatga ttcttgatcc taaaatggat ttaggcgaaa
    12481 tcaaaaacgc actaaagatt gataccgatg atgacgatgt ggaagtaagc cgtgcggcac
    12541 aagctgcaat tgcatacatt agaggggcta tcgggaatga taagccctct ttttataagc
    12601 aagaaaatga aacggttgat ctgattaatt tagctatcct gcaattagca gatcattatt
    12661 acaacgctcg atcagcaacc gtgagtggga acttgcgaga gtacgattta ggttttacaa
    12721 gcctaatctt gcaactcaaa gcaagttatt tgctttttgt ggaggaggag tagcgtatgc
    12781 cccttatcca aacaggaaat ttaaatcaac gcatcaagtt tgtccgagat acgacggtta
    12841 aggatgagga cgggcaagtt gtcccgactt ctacaaccat tcttacttgc tgggcaagtg
    12901 tgcagacaca acgcctgaac gatattaaga cgtcgattgg tacggctttg gaaggaacac
    12961 tgacgttcat tatccgctac caacaaaaat cagagctgac caatgatatg aaagtgcgtt
    13021 ggaatggaaa aacgtttgaa atcattacga ttacgaaagg cgagtttgcg aaggacttca
    13081 cgacaatcat tgcaaaagag gtttcaaaat gagtgtagaa gtcgatgcaa ccgaagtgta
    13141 caaagcgctt agggaagtaa aagcaaacgt tcaacgagtg gaaagcccag cacttagaaa
    13201 agctggggag tacgctcaag aagagttacg acaaaacaca ccttactggg atggaacgaa
    13261 gtcaaacggt aaacgtggtt cgtatatgca agaacatgct aagaaccatg tggttacaag
    13321 ctcgataaaa aacggattgg tagaagtcgg ctatgacaaa gatgtttctt ggcggatgca
    13381 ctttatcgag ttcggaacaa tcaaacaacg tccaaaaggt ttcgtacaaa aaacacaaaa
    13441 gcaaatcgaa aaacaagtaa cacaaatcat tgctgacgaa gtaaaaagga ggctaggact
    13501 ttgaaaacgg cagtatcaca agtctattca attctgaata gcaatgaaaa aacaaagaac
    13561 attgattttt acaccaatag tgttccggaa tcagctcaaa cagtacctag ccttccagtt
    13621 ggcagaatta cagagatatc cggcaactat gaagattttg caagcaacaa tcctttgacc
    13681 attcaattta acgtacaggt agatgtatgg gtgtcaacca tgaaagaggt tgatgccttt
    13741 tattttgccc ttgatgaggt tatgaggggg aatggttggc aatgcgcata cacggaacaa
    13801 acagatgacg aggacttgga aggtgcaaag cggattatca aacgatatgt agcaaatatt
    13861 tcactaaatt aaaaggagag aaaatagatg gcaacagtag gattcgagag cgtcattttt
    13921 ggcgtaaaaa caggtgcagg cggcactcta aaagaattag tagcagataa gtcgaaaggc
    13981 ggagcgatcg aagctaaaat tactggatta ggcgcaactt ctaacacaac atacgcttca
    14041 aacgtaccgt tcttcattgc aagtaaaggg gtttcgtcgc caaaagttac gcttgacgtg
    14101 gcagacttaa tggataacgg catttacagc gaaatcattg gtgctaaaac cgtggatggt
    14161 gcaaatgtaa ttggttcaga aactgaagcg ccttacgtgt cggtagtcat ggttacagcg
    14221 aacaaagaag gaaaacgctt gttcatggga ttgacaaaag gaaaattcag tcatccagat
    14281 atcgacatga aaacagctga agacaaaggg gtagaattgc aaaccgattc catcgaaggg
    14341 gaattcattt ctgatgaacg tggctatgta tacttaacag ccgtagaatc agaaggaatg
    14401 accttacaaa aattcaagga cttggtaaat aacaaagagg gggagtagtt aaccctgcat
    14461 ctacaccaac gacagataca gggacaccaa aagaaccaga accaaaaatt gatacacaag
    14521 gttagccatt tttggctagc cttatttttt gtaaaaacaa ggaggaaaac aaatgattga
    14581 attgcaattg aaacttgacg gaaagaaaaa aacattcaaa caacaagata tttccgcacg
    14641 tgcaatgcgt gagtgtatca aattttacga gaaagcggaa aaagcagacc taactgattt
    14701 agaagcgatt gattcaatga ttgcaattac agcagatatt ttccaagatc cagcagttac
    14761 atttgatgct attttagacg gtttgactgc gagcgagtta gtaccggcat tagaaagtgt
    14821 ttttgaacaa ataaatgaac tgggaaacaa tgaaaaaaag cagacggcga gcaaaaagaa
    14881 ataagttttt ctgaagctag gaaagcaatg gatcaaatct acaaagattt aatcgaagca
    14941 ggttggacga tgagagatgt ggacgaagcc gactatcatt atttgttaca cctttttgga
    15001 gaagtggaga gtggcgaaga atatgtagat ggtgctgatt tcatcaaaca atttttatcg
    15061 gctgaagact tagtaaaact tgaggaagga ggtaaataat ggcaggaaaa ggacaaccgg
    15121 caggaaatat caagctaggg attagtttag atagcactag ttttggtaac acgctggacg
    15181 aaatcaatgc gaaagtcaaa caagctgagt cgaatatgcg tgccaatcta aaggcttatg
    15241 attcagcagg acgttcatac gaagcactta gtcaaaagac gaaagacttg tctacggtta
    15301 tggaagggca aaacgccaaa gtaagagaat taacaaagcg ccgtgatgaa gcgattagca
    15361 agtatggcga ggaatcgaaa caagttgcta accttaacac acagataaac aatgctaccg
    15421 caaaatataa tgcttacagt cgccagttga acgacacaaa aaaagaattg gtgtattcca
    15481 aaacagccgt caatgattta tctaatgaaa tcaaagaaaa tgaacgacaa atgaacgccg
    15541 aagtaaaagc gttgaaagcc gctggtgatg aatctggtgc ttttgaagca aaacaaaaag
    15601 ggctagccaa acaaacggaa ttatccgaga aagctatcga agaacagcgc aaagttgtga
    15661 aactgatggc tgatgagttt ggcgattcag caaatgaaac cgaagatgca aaaagggcat
    15721 tagaaaagtt agaacgacaa agccaaatat ctagcaggca attagaagca ctcaaaagct
    15781 ccagcgatca atcaggaaaa gaaatagaag attttggcga caagtccaca aggtcagcta
    15841 ggaaactgga cggactaaaa gacaaattaa gctcgctaaa aagcgcattt tcgtttggtg
    15901 cagttgctgg attagcgcat aacgctatta gcagtgtagt aagtggcgtg caaggtttgg
    15961 ttggcgaagc agtaaacgca tcggattcat tgatgaagtt ttccaaaacc atggagtttg
    16021 ctaactttgg gaagtcacag atagaaagct cgaaaaaaga aatgaaagac tacgccgata
    16081 agacggttta tggtttagaa gaaattctga acacaaccgc acaattggca tctaatggga
    16141 ttcctaacta tacagaacta accaaggcgg caggtaactt gaatgccgtt gcaggcggtt
    16201 ctagtgatac attcaaatcc gttgccatga tgctaacgca gacggcagga gctgggaaac
    16261 taacaactga aaactggaat caattagcag atgcgatacc gggtgcttca ggattgttac
    16321 aagacgctat gttgaaaaac ggagcttata caggaaactt ccgtgatgca atggcgcaag
    16381 gtcaaatcac ttccgacgag ttcaaccaag caattgtaca gttaggtatg aatgacggag
    16441 cagttaaggc agccacttct acagacacat tgagcggttc ttgggagcag atgaaatcca
    16501 ctgtaataaa tgggctacaa agtattatag aaaaaatagg cgttgaaaat atcactggtt
    16561 tcatcaacag agtaacaaaa gggattgaaa attctattcc taaaattact caatttatag
    16621 gttggttgag agatattgga acgtggatcg ttgaaaatag agagccacta acatggattg
    16681 tcggaatcat aggcggaatt acattagcag taaaagcatt gaacgtagca agtatgttgc
    16741 tggcaattac tggcggaaca ttggcagccc cttttgtggc gattggtgta gcattaggcg
    16801 cactagcagg tgctttggtg atagcttata caaaatctga aacatttaga aatatagtca
    16861 atgcggcttt tacagctgtg aaaaacgtag ttatgagcgt tatcaataat ttggtggaat
    16921 actacaaaat gttgtggagc gtgttgcagt ggctttggga aaaaataaaa gaatgggctt
    16981 catggattgg caataaattc attgaaatga agaacagcgt tgtgaatacg gttcagaacc
    17041 tatggaatgg cgtgaaaaac ttcttcagca atggcgttgg agatacgtgg aataaggtag
    17101 tcggttgggt aaaaaacatt ttcaacaaag caactgaatt gaagaacaac gtttctgatg
    17161 taatcggtaa cctgtggaat ggtatcaaag acacattccg tagaggtatc gatacggtat
    17221 tcaattggtt ttcagaacta ccaacgaaga tgaagaatgc cattattggc ggtaaaaacg
    17281 ccattgttga tgcgttcaaa agtattttca acgcagcact taaagcgata ggtaaaccag
    17341 ttaacgcaat catccatgga gcttcatggg tactagaaaa attgggcgca gaacctctac
    17401 aagaatggga tgtaccacaa tacgctacag gtacaccagc aggtgggcac ccaatcaatg
    17461 gtccaatgat ggttaatgat ggacgtggag cagaaacagt tatcacacca gatggtagag
    17521 ccttcattcc taaaggacgc aatgttgttc taaacgcacc aaaaggaacg catgtcttga
    17581 cagcagaaga gaccgcgcaa cttcaaggtt ctaaagctcc taagtatcgt tacaaaaaag
    17641 gtactaactt ctttggtaac atgtgggata gtgtgaaaaa ggttgctggt aatgtaggtc
    17701 acacaattaa aaacgtagta ggtgacgtgt gggactttat ttcagacccg ggagcgttag
    17761 ctagaaaagt acttgggggg ttagatgtat taggtggatt gacaaagtat ccattagaag
    17821 taggtaaagg catcctatct aaagcaacaa gtgcactgac tgaaaaaatc actgggttgt
    17881 tctcatctgg taacttagat acctccgtag gaacaaatgg cgtctataaa tatttggctg
    17941 atgttgctaa gtctgtgatg aagaaattcc caggatttgt ggcaactagt gggtatagac
    18001 caggtgaccc ttattcacat ggtaaacgta atgccattga tattgcatta ccaggcgtca
    18061 caggaggctc acctagatac acagaagcag caaactacgc ttttgacaaa ttcgcttcca
    18121 aaattggtta cgtaatcact aatgggaaag ttcgtgaccg ttcaggacaa tcaggtcaac
    18181 cagcaactgg tgcatgggag ccatggcccg atggagatca ctatgatcat gtgcatttaa
    18241 acggtgtgaa agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg
    18301 tagaaagatg gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg
    18361 caaacttaaa tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag
    18421 ttaacaattg ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga
    18481 ttgacccaac attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac
    18541 tatctaacat cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg
    18601 cctatcgtgg agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa
    18661 tcgcagaagg aaacaagcca gaaattatta ttccgttaga taaggctaaa cgatcaagag
    18721 cgatgcaatt attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa
    18781 atgcgacaac tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa
    18841 caacgaatca gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata
    18901 gagatttagc tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat
    18961 tactgagaaa tagaataaac gaagaaattg atttgtccac agaagactat tttgctactg
    19021 ggttgagtaa tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca
    19081 gagaaagcag tgagagcgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc
    19141 atgggtttcg agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt
    19201 ttgaactgga atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt
    19261 taagtaagac tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt
    19321 tcacatcaaa ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga
    19381 aaacacgtgg taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg
    19441 agaaaaaagg tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac
    19501 ggatgtcccc gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa
    19561 tccaaaactc acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga
    19621 ctctagaagt atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg
    19681 tagaatcttc tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccaa
    19741 ctggagaatt cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg
    19801 aggagcgtga tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta
    19861 taacgaacat ttattctcta acagttttga atttggtgtg gacgagatca acgaagaggc
    19921 tagtagcttt acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa
    19981 atatattcct agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa
    20041 aatcagttgt aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc
    20101 aggagataac tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc
    20161 aacgaaacaa ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta
    20221 tcaagctacc gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa
    20281 atacaacgta aaatggtatt tcaagggaat tcaaaacaga aaaatttata caggcatacg
    20341 tgctgtaaat gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt
    20401 gtttgttcag gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat
    20461 gaaagatata gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt
    20521 gacacaagat ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta
    20581 cgatcaaacc gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg
    20641 caatacgtat tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt
    20701 cgaaacgatt gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc
    20761 ggttttgaca gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa
    20821 cataagaagt cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa
    20881 tgtggatgga tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta
    20941 tcacgtttta tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt
    21001 atcttcaagc ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat
    21061 ggtcgttgtt cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc
    21121 attaacagtg gatttgacac aagaagttat acctggatct attcttccag aatcggaaga
    21181 atatgaatgg actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa
    21241 tttaaacaat ggtgataagg tctataattt accactatgc tcagttaatt ctactggatc
    21301 aactgtttca atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat
    21361 tttgtggagg gggactgcgt taatgcatga tactcaaact gtccaacctt caaaaaaaat
    21421 ttggcagaca gtttcaggtt ttttgttagt atggctccca tacgaaaacg ggcaagcaat
    21481 tgaggataga tatgtaacta cgccttttta taaggagcgt gtaactttta ctaatgtttt
    21541 aggagaaatt gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa
    21601 ctataattcg aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc
    21661 aaatatggtg cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat
    21721 ttagaaatta aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt
    21781 agagttggtg atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt
    21841 acaccttttg taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt
    21901 aactataccg agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg
    21961 ttcactaagt caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta
    22021 gatcctaacg gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagtg
    22081 gatattaatt cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa
    22141 atgcaaacca ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac
    22201 tcgaaatata acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt
    22261 attgatcaaa ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac
    22321 agcatcgact ttgggggcta tgattattcg gggaatccga atttgttaag taagctatca
    22381 tacgacttaa ttgaaaatca aaatacttca gctggaacac tttctaaggg tgaaaactcg
    22441 tttaaatata ataagatatc agctgaagtg gaaggtggag tagagttata ttataaacga
    22501 agaggtatag ctaactggtt accctctaat aaaacgcttg taatgactgt taagcttaga
    22561 gctggagcgg actatagtcc agttgacgga aaacttatac tgattagata taggtatgtt
    22621 gacagtggaa ctggcaagat tgttttagac ttacctatta acagtaattc gataactcag
    22681 gaatggaaag agtttagtat tactggaact actccaacat ttagcccaca agcataccat
    22741 ccttggatac aatttagggc tcaagatggg gtacttgggg aaatagaaat gagctatgac
    22801 atcaaaatcg aagaaggctc tacagctaca ccatatcagc ctaacttact tgatgactct
    22861 tactggctag gtaaaacacc gttgggtgag aatttgataa cgaatgccaa gttcccaatt
    22921 ataacacgaa gcaatcctat ttctggtttt gatatttcag aagaattaat aataggtgaa
    22981 acttatacgg tatctttaaa agggactaag ccagctacta aagaattcca cttatactac
    23041 ggagaagcaa attaccagca gttgcagtat caggcaactt tacttcctgt ggaaggatta
    23101 acagatgtgt ggagtgccac ttttacagcc agaaatacta gtgaaacgac aaatttactt
    23161 agagtagcgt tatggcaaaa acctaattct gcaacatatg atactgttca aattgactgg
    23221 ctcaagattg aaaaaggcga cacacgcaca ccaaatatca gtcagtttaa atactttggt
    23281 gaaggcttga aagacagtaa caatccgaac gactacagct gggatgtcac accagagtat
    23341 actgaaaaaa gcttgaataa tacggttagt ctgactgaac cgcaatccat cgaaggattg
    23401 aaaaatttcg aagatggtat tcaatcaaaa ggaaaatctg tattgacatc agacgacaac
    23461 aaatatgaag tcgtaacctt aacagttaca aacgggaata ccggatcagc aaagctttat
    23521 cgtgaaggaa aaaccgtcac tatttatttt tttgcgttga atgggaaaag cagtggtgga
    23581 aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa
    23641 ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat
    23701 ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact
    23761 tacacgattt agaaaggcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta
    23821 cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt
    23881 cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa
    23941 aacatttgga aatatggccg tactggcgga gagtatgcag gaaaagtatt ggacgacatg
    24001 cttgtatccg ttccttacac agatcagcca ccgctcgaag gggttcgttc agatggcgaa
    24061 ccgctaacga ttgctgatca gatgtttgat cctaaattga accaatggat tattttagcg
    24121 aacgcgttag atcacaacga tttaaacaat ctcaaagcga tgtacgaggc tcttgaacat
    24181 gaaaacggca acctaaaaca gctaaatgcc aaactcatgc taagcgatgt agcgattaaa
    24241 caggaaaata ctgcattgaa agaaaaagct gacagtttag cacaaatcaa ttcaaaaatg
    24301 atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca
    24361 gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg
    24421 gggctgtttt actgacgatc aagttcgtct attcgttcca ctatgtatca cagatgaaga
    24481 agcagataga attattagca aagaagagag cgcatcttaa gtgatgcgtt tttttgttgg
    24541 aaagttggtg gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga ttagtaagaa
    24601 ttgaaacaaa attagacaac tacgaatcac ttagagaaaa agctgatagt gcaaaagatt
    24661 tggcagacaa agcctactca gtagcgctaa acaacgcaga agatatcaaa gaaatgaaag
    24721 ccaataataa atggtcgtgg ggttacatga tcggtttagg cattacgatc attggctatt
    24781 tcttgactaa attgtaaagg agttaagaag aaatgatttt acccgataag tattatcaag
    24841 tcattaaatg gacggtttta acagtattac cagctgcatc tgttttagta gccacgttag
    24901 gaaaagcgta tggatggaat ggaacagata tgacagtact cactatcaat gcagtagcaa
    24961 catttttagg cgttatcact ggtgtgtctg catataactt aaaaaaatag gaggaaaaaa
    25021 atgaagaaga aaatcatttt atcattgagc ctaataatgg ctcttttttt attgccaatt
    25081 aacgggttcg cctatacgat caacaatgaa tttaatttag gtgtaaatga aggtagctca
    25141 caagtagcaa ataaccaata catcctattg catgaaacag caaacgaaac tgcgacagga
    25201 cgaaatgaag cgcagtacat gaaacgttct tggtacaatg cctatacagc gtatattgtt
    25261 ggcgatggtg ggatcgttta ccaagttgga caacctggtt atgtacaata tggcgctggt
    25321 tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc aacatacgca cgataaagta
    25381 acgtttgaaa agaactacaa ggcatatgtt gaattagcga gagattccgc taataaatac
    25441 ggtattccac ttacattaga cacaccgtat aatcaaccag gaatcaaatc acatttatgg
    25501 gtaactcaaa acatttgggg cgatcacact gatccgtatg gatacttatc agaaatgggt
    25561 gtaagtaagg aaaaactggc ttacgattta gctcatggat ttacagacga aaacccaacg
    25621 acttctgaga acaagcctgt cattgatcca acccgagcag gtgcagcaaa tccaacttta
    25681 tcagatggaa cgaaccattc tcacattgat cagtttggag aaatcgaaaa tgcgaactta
    25741 cacgtcgctg gatggcacat cgctaactat aaatacgagt atattttcat tatggactac
    25801 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc ggatgtaaat
    25861 caagcttata atacttctgg aaacgttggc tatcatgtat cttttaacat gcgcaatttc
    25921 cctaataaga aagtatacgt catgatgcgg gcaacgaatg atccagaagg aaacactaaa
    25981 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
    26041 tagctcctcg ttgaggagca gtacatagaa ttgaaaatta acgttaaatc aaaaaaatat
    26101 ttacaagatg tttatttaag tagataataa aagaaaaaag caggtgaaat tgtgacagca
    26161 gaaattggta ttatgaataa aagtggaatc gtgttagctt cagattcagc atctacgatt
    26221 ggagatagca aagtatataa tactgcaaaa aaattattta ctttggattc catgcattct
    26281 gtaggtatca tgatatatgg taatgctgaa tttaatggta ttccttggga aataatcatt
    26341 actcaatata agaaaagcat tggtagttct gtttttaata ctttggaaga atacgcggat
    26401 aattttatag aatttgtcaa aacagcatct tttattagaa gtgaacagac cgagcaagaa
    26461 caaatgattg gggtttttca aaaaataatt tcaggtttat ttgagagtat agaagtagat
    26521 attaactttt taataagcca aggtacacaa atagacaaag atgtcctagt gaagttatta
    26581 caaacaaaaa tgaataccaa tttgtctcaa cagtcccaga cattcatttt agatattgaa
    26641 aaggcgttat ttcttagtaa ctatggagaa atattaaaag atattcttaa tagtatttct
    26701 acaatggaag gtgtttcaga agctatatca gaggaaattc aaagttacgt ttatgaaatt
    26761 ataattagag acgatgttta ttcttcccct acaggaatag ttatagcagg atatggaaga
    26821 atggatattt tccctaaact atattcttat aatatgtttg gatttgttat gaatattctg
    26881 aaatactcag aatatgaaag tgctcaaata ggaaatgaca atggttcatt aagatctact
    26941 atattaccat ttgctcagtc tgatgtggta aatactgtag ttcaaggggt agatccacaa
    27001 attactaatt atctgtcgag tcaagtcgat agttttgacg ataacggaaa aaatacctat
    27061 attaatatta taaagaatat ttcagagttt caacaaaatc aattcatatt tccgttacta
    27121 aacatgattg ctttactccc agttgaagaa acagcaatta tcgcagagac tttattaaat
    27181 ttaaccagtt ttaaacgaaa atatactact tctgtagaaa cagtaggtgg cccaattgat
    27241 g
//
LOCUS       DACCVU010000029        26673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCVU010000029 DACCVU010000000
VERSION     DACCVU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.029"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(95..1327)
                     /locus_tag="IVW29_001082"
     CDS             complement(95..1327)
                     /locus_tag="IVW29_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7838579.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1582..2151
                     /locus_tag="IVW29_001083"
     CDS             1582..2151
                     /locus_tag="IVW29_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838580.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2329..2769)
                     /locus_tag="IVW29_001084"
     CDS             complement(2329..2769)
                     /locus_tag="IVW29_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7838581.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2927..3691
                     /gene="map"
                     /locus_tag="IVW29_001085"
     CDS             2927..3691
                     /gene="map"
                     /locus_tag="IVW29_001085"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7838582.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3723..4646
                     /locus_tag="IVW29_001086"
     CDS             3723..4646
                     /locus_tag="IVW29_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7838583.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4722..5861
                     /locus_tag="IVW29_001087"
     CDS             4722..5861
                     /locus_tag="IVW29_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7838584.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5854..6654
                     /locus_tag="IVW29_001088"
     CDS             5854..6654
                     /locus_tag="IVW29_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7838585.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6654..7481
                     /locus_tag="IVW29_001089"
     CDS             6654..7481
                     /locus_tag="IVW29_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7838586.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7459..8193
                     /locus_tag="IVW29_001090"
     CDS             7459..8193
                     /locus_tag="IVW29_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7838587.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8293..9159
                     /gene="rfbA"
                     /locus_tag="IVW29_001091"
     CDS             8293..9159
                     /gene="rfbA"
                     /locus_tag="IVW29_001091"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7838588.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9173..9745
                     /gene="rfbC"
                     /locus_tag="IVW29_001092"
     CDS             9173..9745
                     /gene="rfbC"
                     /locus_tag="IVW29_001092"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7838589.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9767..10795
                     /gene="rfbB"
                     /locus_tag="IVW29_001093"
     CDS             9767..10795
                     /gene="rfbB"
                     /locus_tag="IVW29_001093"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7838590.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10893..11744
                     /gene="rfbD"
                     /locus_tag="IVW29_001094"
     CDS             10893..11744
                     /gene="rfbD"
                     /locus_tag="IVW29_001094"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7838591.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11778..13811
                     /locus_tag="IVW29_001095"
     CDS             11778..13811
                     /locus_tag="IVW29_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838592.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13855..15134
                     /locus_tag="IVW29_001096"
                     /pseudo
     CDS             13855..15134
                     /locus_tag="IVW29_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15344..16150
                     /locus_tag="IVW29_001097"
     CDS             15344..16150
                     /locus_tag="IVW29_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838593.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16162..17382
                     /locus_tag="IVW29_001098"
     CDS             16162..17382
                     /locus_tag="IVW29_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838594.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17372..18958
                     /locus_tag="IVW29_001099"
     CDS             17372..18958
                     /locus_tag="IVW29_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7838595.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18997..21135
                     /locus_tag="IVW29_001100"
     CDS             18997..21135
                     /locus_tag="IVW29_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7838596.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21503..22486)
                     /locus_tag="IVW29_001101"
     CDS             complement(21503..22486)
                     /locus_tag="IVW29_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7838597.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22864..24255
                     /locus_tag="IVW29_001102"
     CDS             22864..24255
                     /locus_tag="IVW29_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7838598.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24271..25311
                     /locus_tag="IVW29_001103"
     CDS             24271..25311
                     /locus_tag="IVW29_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7838599.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25330..26415
                     /locus_tag="IVW29_001104"
     CDS             25330..26415
                     /locus_tag="IVW29_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7838600.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26448..>26673
                     /locus_tag="IVW29_001105"
     CDS             26448..>26673
                     /locus_tag="IVW29_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7838601.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEI"
BASE COUNT         8542 a         4440 c         5421 g         8270 t
ORIGIN      
        1 tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt attctatgct
       61 ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa
      121 tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca gatcctacca
      181 tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct tcatcggaca
      241 tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag tgattcgatg
      301 cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt
      361 caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca agcagttttg
      421 ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct ttaaaagtaa
      481 atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt gaaatgtaac
      541 catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata tttgccatga
      601 atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt ttaggcaatc
      661 cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt tctcgattca
      721 attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag acagggtctt
      781 cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact tgtttttctt
      841 ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc acgacagtcc
      901 aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg ccattcgctg
      961 attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga tcggcagaaa
     1021 caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg attttagatt
     1081 gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt tggatctggt
     1141 cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct tgtgtgatca
     1201 atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt cctttttctg
     1261 tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca ttaaaatcag
     1321 ataacataat aaaaattcct cctcaccatt ttttctggta actattagca tgataccatt
     1381 taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt tttaaggtta
     1441 agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc aaaaactaat
     1501 tttgcatatc aaaaaaaaca tgttacaatg aaattaatca atggtggaag cgttataaaa
     1561 aaattaagga gattaaaaaa tatggcaaga aaaaagacga ttactagaga acagattttg
     1621 gcagctgctt atgaagtagc tgccaccgat ggatttacac gatttactgc gagaaacatc
     1681 gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg agtttaaaaa tatggatgac
     1741 ttgagacaag ctctttttga gcagatctat gatcatttac aatatgaagt gttcactgtt
     1801 gagcatacag ggaatacgat tatcgatctt gcttttaatt atatccattt tgcaagaaga
     1861 gagaagaagt tgtatcgttc actgtactta gaagaatatg gtggaggaaa agagatgcag
     1921 aatttctctt atacttactt tgtgaataca gtgaaacgcg atccagctta tgtgaatctt
     1981 tcagaagagc agatcgaatc tttgtataat gggacatgga tcatagctac tgggatcgca
     2041 gctttgatgt cttcaagcat catccatcca actgatgaac agattgaaaa gatgatccaa
     2101 gacagtatag atgctatctt gaaattcaaa gaaccaatcg aaatcgattg aatatactca
     2161 aaagttccac aaatcagtag gtaacaagag atgtgtcaaa ataaggaaaa gttcgttttt
     2221 cttacagcgt atgcattttt aactcccgaa gaactcttcc atgaatgcaa tgaataaaag
     2281 agccatacag attgaataaa tctggatggc tcttttattg caagtatatt aaatttttgc
     2341 gactagttct ttagcgaaag cttctaaatt ttcgatatct tcttcttcag cattcagatc
     2401 cactttaaca ttttcggctc cttttgatgc gcctgttttt gcaaaagcag catcgaaatc
     2461 atccaccgat ttgcagaatt catcgtaaaa agtatcacca gatccgcaga caccgtaaat
     2521 ttttcccaat agatccaatt cttgcaagtc ttcataaaag tctacgatct catcaggcag
     2581 atcgccatca tcgtatgtat aagtagctac tacgcaaata tctgcctcct cgaattcatc
     2641 tgcatctact tgggtacatt cgttgatttc gacttcgata tccagatttt caaatgcttc
     2701 tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg ctagcatata cgatttttgc
     2761 taaagccatg atgtgcctcc tcgtttataa ttatttaaca attatgaatt ataccaaaac
     2821 atcattgaaa agaaaataaa acctagagac acctggggaa tgtcatttgg ctaaatgtat
     2881 gccgctatgt taaaatgaga gggatttgta aaaaggagac gaaaatatga ttactttaaa
     2941 atcagcacgt gaaattgaaa tgatggcaga atctggagct ttattagcag atgtacataa
     3001 aaatctgcgt gattttataa aaccaggaat cacaagttgg gatattgagg tatttgtcag
     3061 aaactatatc gagagtcatg gcggaattgc tgcgcaaatt ggctttgaag ggtatgaata
     3121 tgcgacttgt atcagtataa atgatgaaat ttgtcatgga ttccctcgta aaaagccttt
     3181 gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat ttgaaaggcg gcatttctga
     3241 ttcttgttgg gcctatgtag ttggagaatc tacacctgag atcgaccgat tgatggaagt
     3301 aacgaagaaa gctttgtaca tcggtattga gcaagcacaa gtagggaatc ggattggtga
     3361 catcggtcat gctatccaaa cttatgtaga aggcgaaaat ctagcgatcg ttcgtgattt
     3421 tattggtcat ggcgttggtc ctacgattca tgaagaacca gcagttcctc attacggaga
     3481 agctggaaaa gggttgcggt tgaaagaagg catggttatc acgatcgaac ctatggttaa
     3541 taccggaact tggaaaatga aaatggatcc taacggctgg actgcgtata caagagacgg
     3601 cggactttct tgccaatatg aacatacatt ggctattact aaagatggtc caagaatttt
     3661 gacatcacaa ggagaagaag gcacttattg atctatccag tcaataggta cgggagaggt
     3721 acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct gatgcgcttt attgagacga
     3781 ctcaaaagcg catcactgat tcagaaatga gtacgacctc tgtcgtcgtt gcttattatt
     3841 tactcttgtc tttatttcca ttgattattg cttttggtaa tattttaccg tttctccaca
     3901 tcgatcaaga gactgtactg acctatttac gagaagtgat tcctgagacc gtttaccagt
     3961 ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc aggaggcttg ttatccgttt
     4021 ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa tgctttgcaa attgcgatga
     4081 ataaagcgta tggcgtagaa aatagaaaaa attttttgat cgtccgtttc ttctcactaa
     4141 ttgtcattgt cttgttcatg attgctatta gtggggtaac tatcgttctt gggttgggac
     4201 agctgatttt ggatgctatt cagccaatcg tacaaatacc cgtgaacttt atcgatcagt
     4261 tccaaacttt gaagtggcca atcacattgg tagctttatt tgtcatcatg ttcttgattt
     4321 atttaattgt ccctaatgct cagttgaagt tgaagacgat cattccgggc acgatcttcg
     4381 ctactgtcgg ctggatgctg ttatcacaag tgtttagtat ctatgctagg tattttgcaa
     4441 cgaggatcag cggttatcag atcataggaa gttttattgt actcatgctt tggcttaatt
     4501 ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc agttgttcaa gaatacgtga
     4561 cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa atggttcaat aaattgaaaa
     4621 ctaaattttc tcataaaaaa aagtagaaac aagagttctt attgtaaatt tatatcagta
     4681 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
     4741 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
     4801 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
     4861 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
     4921 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
     4981 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
     5041 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
     5101 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
     5161 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
     5221 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
     5281 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
     5341 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
     5401 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
     5461 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
     5521 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
     5581 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
     5641 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
     5701 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
     5761 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
     5821 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
     5881 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
     5941 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
     6001 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
     6061 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
     6121 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
     6181 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
     6241 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
     6301 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
     6361 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
     6421 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
     6481 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
     6541 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
     6601 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
     6661 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
     6721 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
     6781 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
     6841 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
     6901 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
     6961 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
     7021 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
     7081 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
     7141 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
     7201 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
     7261 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
     7321 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
     7381 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
     7441 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
     7501 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
     7561 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
     7621 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
     7681 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
     7741 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
     7801 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
     7861 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
     7921 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
     7981 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
     8041 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
     8101 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
     8161 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
     8221 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
     8281 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
     8341 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
     8401 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
     8461 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
     8521 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
     8581 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
     8641 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
     8701 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
     8761 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
     8821 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
     8881 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
     8941 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
     9001 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
     9061 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
     9121 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
     9181 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
     9241 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
     9301 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
     9361 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
     9421 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
     9481 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
     9541 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
     9601 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
     9661 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
     9721 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
     9781 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
     9841 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
     9901 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
     9961 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    10021 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    10081 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    10141 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    10201 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    10261 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    10321 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    10381 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    10441 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    10501 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    10561 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    10621 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    10681 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    10741 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    10801 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    10861 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    10921 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    10981 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    11041 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    11101 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    11161 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    11221 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    11281 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    11341 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    11401 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    11461 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    11521 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    11581 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    11641 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    11701 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    11761 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    11821 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    11881 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    11941 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    12001 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    12061 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    12121 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    12181 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    12241 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    12301 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    12361 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    12421 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    12481 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    12541 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    12601 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    12661 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    12721 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    12781 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    12841 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    12901 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    12961 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    13021 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    13081 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    13141 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    13201 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    13261 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    13321 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    13381 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    13441 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    13501 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    13561 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    13621 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    13681 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    13741 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    13801 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    13861 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    13921 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    13981 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    14041 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    14101 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    14161 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    14221 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt ttatcgtgta tttacagaga
    14281 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    14341 tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    14401 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    14461 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    14521 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    14581 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    14641 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    14701 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    14761 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    14821 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    14881 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    14941 cattgacttc ataaccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    15001 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    15061 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    15121 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    15181 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    15241 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    15301 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    15361 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    15421 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    15481 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    15541 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    15601 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    15661 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccattt
    15721 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    15781 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    15841 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    15901 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    15961 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    16021 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    16081 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    16141 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    16201 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    16261 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    16321 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    16381 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    16441 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    16501 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    16561 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    16621 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    16681 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    16741 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    16801 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    16861 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    16921 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    16981 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    17041 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    17101 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    17161 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    17221 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    17281 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    17341 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    17401 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    17461 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    17521 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    17581 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    17641 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    17701 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    17761 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    17821 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    17881 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    17941 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    18001 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    18061 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    18121 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    18181 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    18241 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    18301 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    18361 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    18421 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    18481 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    18541 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    18601 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    18661 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    18721 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    18781 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    18841 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    18901 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    18961 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    19021 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    19081 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    19141 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    19201 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    19261 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    19321 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    19381 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    19441 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    19501 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    19561 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    19621 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    19681 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    19741 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    19801 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    19861 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    19921 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    19981 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    20041 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    20101 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    20161 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    20221 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    20281 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    20341 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    20401 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    20461 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    20521 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    20581 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    20641 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    20701 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    20761 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    20821 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    20881 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    20941 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    21001 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    21061 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    21121 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    21181 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    21241 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    21301 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    21361 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    21421 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    21481 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    21541 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    21601 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    21661 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    21721 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    21781 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    21841 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    21901 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    21961 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    22021 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    22081 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    22141 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    22201 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    22261 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    22321 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    22381 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    22441 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    22501 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    22561 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    22621 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    22681 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    22741 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    22801 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    22861 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    22921 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    22981 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    23041 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    23101 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    23161 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    23221 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    23281 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    23341 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    23401 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    23461 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    23521 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    23581 gaaacatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    23641 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    23701 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    23761 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    23821 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    23881 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    23941 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    24001 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    24061 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    24121 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    24181 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    24241 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    24301 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    24361 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    24421 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    24481 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    24541 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    24601 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    24661 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    24721 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    24781 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    24841 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    24901 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    24961 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    25021 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    25081 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    25141 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    25201 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    25261 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    25321 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    25381 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    25441 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    25501 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    25561 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    25621 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    25681 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    25741 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    25801 tttcaaaacc atccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    25861 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    25921 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    25981 ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt
    26041 agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta
    26101 ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct
    26161 ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc
    26221 ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta
    26281 gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata
    26341 acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga gagtatatat
    26401 agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg aaaatatgtt
    26461 atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat cgattgaacg
    26521 aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca tttattgaag
    26581 catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt
    26641 ggggtggttt ttccattgta caagcagaaa tca
//
LOCUS       DACCVU010000030        26224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCVU010000030 DACCVU010000000
VERSION     DACCVU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.030"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..955)
                     /gene="mdcB"
                     /locus_tag="IVW29_001106"
     CDS             complement(86..955)
                     /gene="mdcB"
                     /locus_tag="IVW29_001106"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7838602.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(980..2056)
                     /locus_tag="IVW29_001107"
     CDS             complement(980..2056)
                     /locus_tag="IVW29_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7838603.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(2080..2673)
                     /locus_tag="IVW29_001108"
     CDS             complement(2080..2673)
                     /locus_tag="IVW29_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7838604.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(2677..4353)
                     /gene="mdcD"
                     /locus_tag="IVW29_001109"
     CDS             complement(2677..4353)
                     /gene="mdcD"
                     /locus_tag="IVW29_001109"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7838605.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(4343..4642)
                     /locus_tag="IVW29_001110"
     CDS             complement(4343..4642)
                     /locus_tag="IVW29_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7838606.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(4744..6390)
                     /gene="mdcA"
                     /locus_tag="IVW29_001111"
     CDS             complement(4744..6390)
                     /gene="mdcA"
                     /locus_tag="IVW29_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP7838607.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(6397..7308)
                     /locus_tag="IVW29_001112"
     CDS             complement(6397..7308)
                     /locus_tag="IVW29_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7838608.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            7428..8285
                     /locus_tag="IVW29_001113"
     CDS             7428..8285
                     /locus_tag="IVW29_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7838609.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(8472..8834)
                     /locus_tag="IVW29_001114"
     CDS             complement(8472..8834)
                     /locus_tag="IVW29_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838610.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            9180..10619
                     /locus_tag="IVW29_001115"
     CDS             9180..10619
                     /locus_tag="IVW29_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7838611.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(10626..13178)
                     /locus_tag="IVW29_001116"
     CDS             complement(10626..13178)
                     /locus_tag="IVW29_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7838612.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(13292..14623)
                     /locus_tag="IVW29_001117"
     CDS             complement(13292..14623)
                     /locus_tag="IVW29_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838613.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(14589..16067)
                     /locus_tag="IVW29_001118"
     CDS             complement(14589..16067)
                     /locus_tag="IVW29_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838614.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(16372..18207)
                     /gene="tdc"
                     /locus_tag="IVW29_001119"
     CDS             complement(16372..18207)
                     /gene="tdc"
                     /locus_tag="IVW29_001119"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7838615.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            18677..20104
                     /locus_tag="IVW29_001120"
     CDS             18677..20104
                     /locus_tag="IVW29_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7838616.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(20196..20561)
                     /locus_tag="IVW29_001121"
     CDS             complement(20196..20561)
                     /locus_tag="IVW29_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7838617.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(20558..20923)
                     /locus_tag="IVW29_001122"
     CDS             complement(20558..20923)
                     /locus_tag="IVW29_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7838618.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(21115..21363)
                     /locus_tag="IVW29_001123"
     CDS             complement(21115..21363)
                     /locus_tag="IVW29_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838619.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            21362..21490
                     /locus_tag="IVW29_001124"
     CDS             21362..21490
                     /locus_tag="IVW29_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7838620.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            21547..23241
                     /locus_tag="IVW29_001125"
     CDS             21547..23241
                     /locus_tag="IVW29_001125"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7838621.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            23308..23514
                     /locus_tag="IVW29_001126"
     CDS             23308..23514
                     /locus_tag="IVW29_001126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838622.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            23892..25025
                     /locus_tag="IVW29_001127"
     CDS             23892..25025
                     /locus_tag="IVW29_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7838623.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            25056..25376
                     /locus_tag="IVW29_001128"
     CDS             25056..25376
                     /locus_tag="IVW29_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838624.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(25405..25869)
                     /locus_tag="IVW29_001129"
     CDS             complement(25405..25869)
                     /locus_tag="IVW29_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838625.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(26001..>26224)
                     /locus_tag="IVW29_001130"
     CDS             complement(26001..>26224)
                     /locus_tag="IVW29_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7838626.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         7988 a         5431 c         4545 g         8260 t
ORIGIN      
        1 aaaaaactat ttttatgata gccttgctaa aaggaagagt caataaccag atgcataaac
       61 tgttttttta gcaattttgt cttatttagt cattgagtaa catcggacaa atgtatgtgt
      121 cggcagtcca taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc
      181 ttcctccagg agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat
      241 ttggtaaagc actttgacta gcagcatgag caagtttttg catttccact aacgttttct
      301 gatcactgcg atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat
      361 gcatttgttt ctgtacatat gaatcattat ctagctcctt agctgcttga attgcaagcc
      421 tgatatgcgg ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct
      481 tcaccctatg accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa
      541 gcttgctgac atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt
      601 gtgcaatgac actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc
      661 catttgtagc aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc
      721 ttaactcctg atcaatagca tgatgccatg ctatttgagc catttcttca aaaaaaggag
      781 ttagcgtctt agcagactta agaaataaag catagtccat atcggaatgt gcacccgcat
      841 ccagtgcatc cactaatcca ggttttggac ttaatgttac ttctgcgatc aatgcttcct
      901 ctaccaacgc agcgagttgc tgagtcactg ctttttttcg ttcgtcattt atcatgacac
      961 tcactccttc ttcctactat tacttgctga aacctttttt gatagctacc caaatgatat
     1021 gaagtaacca gcttaaagca aatgcacaac ttgccgtcat tagataagct acaagaatca
     1081 tgactcctga aggtaactgc agccaactat ctttaaacaa taataacagt acttggaaaa
     1141 caaatacatt ggctaaatac gtacgataag cataaacaga caaccatttg aacaatggca
     1201 attttggcga atcgtttaca atcattttgg aagcgcctat gaagacagca aagatgatga
     1261 ctaaactgta taaactttgg atcgtattca agtaaggtgc attcgcaaag ctcaagtctc
     1321 ctggatgtgc gagcaatgaa cggacagaaa agaacgcgat aactaaggca acaggtaaga
     1381 aaagataacg tactctgtcc aaatattgat agactttttc gtgataaaca aatgcggcta
     1441 ccccataaat catgaaacta gcaacaaacc gatctaataa gtaccagctg tcatgataag
     1501 gcccagtcaa tacttgtgtt tgatagaaca gataccatgc gacatataaa atcgttgaac
     1561 caatcaatac tggccataca tattttttat tagcaaatac tttgtaaccg agccaaacaa
     1621 aaaacggcat gaacaattgg atctgcagca tcataacggt ataccataaa tgaggcgctc
     1681 catcacctgt gacatatttc agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt
     1741 ggactgctgg aaatagccaa agataagccg tcgtccatag aagatacgga acgactagtt
     1801 caaaaaactt ttctttcaaa aattcccaat aagacaaatg ctgacttgtt tttaccatat
     1861 tataaacaat tgcaaaaata aacattggtg ctgtgtattt aacgatgacg taaaaggtac
     1921 caagaaaagc tgtatttgtt gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt
     1981 gcatcataac agccgttaca gctgatactt tctggaagtc acttacgttg attttgattt
     2041 ttttgttttc aaacatctct ttctctctct ttttctttat taccaaagtt ctttggttaa
     2101 tttaggtcct tcgactgttt tcaatagaat ttttttctct ggctctctca aatactcttt
     2161 tagtgaaaaa ccgccttgcc gagtcactac ttgggtatcg aattgctcaa aaaaagcagg
     2221 atgcgattga attacctgaa ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc
     2281 aaaatcactg gtttctttta ctgccggaat cccagtcgat aattcaaatc ctaagctgcc
     2341 gccgattccc catttacatc ctgataatat gtttctagca gcatgaaatt gctgataaac
     2401 aggataatcc atgattttct cttgtctata cgattcttgt tcgatgatgt cccacggatg
     2461 aatgacttgt ttgacggcat gttttgagat ttctgttgac catctctgat tgcgggcagg
     2521 tccacgtaaa ccaatcggta gtatttctgc gtgatccgtc gacgcacgac gaactgttcc
     2581 gaacatcttt tcagttagaa cgacccattc aggcaaggtg aacccttcta tttcatgttt
     2641 atcaaaaagg acaatatcat gtgcttgaat cattttttat gcatcccatt cttcgtccat
     2701 tttggcacgg actttatttg tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta
     2761 tcgatttgtc agcatcgtgt catttgtatt gcggacatct tgaatggctt cattgattgc
     2821 ttcgatgact ttgctgacat tttgttctga agcagcttgg tcggtgatat cttctaggaa
     2881 atgatagatc gctcctagtt ttgtataatt attgatatcg taagcgatag ctggaacttt
     2941 ttcagcagct gcttcgattt cagcaaccga acgttttgtc acacgtgcag cactttcttt
     3001 agacatcgct tggacggtaa tttctttatt gtttaaagca atcaagcggc ttgattgtaa
     3061 tccatgtgct aaaaatgcac ctgaaatagc atttcctgca atcaatccaa taactggatg
     3121 tccagcttga cggagttttg cataagcagc agcactatta gccaaagcca catggatacc
     3181 gatcaattct tcgttatagc cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg
     3241 ccgtttcact tttttgtctt tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg
     3301 gaaaccttca actaaaccaa cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc
     3361 aggtacgatt gatgtcacga tggcttcttc tatgaattta cttgatttac cataatacac
     3421 agtactaata gaagaagttg gatttttcat gccagttagt gccgcaaacc aatcgtaacc
     3481 aacacttgct gcagctggtt catttccttc tgctacttct ggtaagtcaa ttgattttgc
     3541 ttgatggtta gcaattgctt cattgtaatc gtcaatggcc attggttttg aaagatctaa
     3601 agaatcaagt aatgataaat aaaatgctga gctttcactg cgatgcgtgt cttttcgttc
     3661 gtccattgct gcgatgacag cagctttgat tgcttctaca ctatcagtca ctaattcatc
     3721 aatgattttt gattctaccc gttgacgaga tcctaatgta ttccaaatca agtctttgtc
     3781 actagaatca aattctcgaa cgcctgcttc ttgttcaata acttctggtc cattcaaacc
     3841 gactctcgct ttttttgttg cgattaaata actcattaat gctgaagtaa tcgacatacc
     3901 gccaaatgat cctacacgtc ctggaatcaa accgatgact ggtacatatt gttttagagc
     3961 aatgattaca tttccgattt ctgaaataga taacagtccg tagtttgctt cttgcaaacg
     4021 aacaccacct gtatctagta caatgactgg aaagatctca tttccttttt tattttcttc
     4081 taacacatgt tcaagtgcag caacgatttt tgcaccggaa acttcaccga taccgccacc
     4141 ttggaatttt ccttccatag cgattactac tgcttgtttg ccattgatcg tgccacggct
     4201 gacaacgatc ccgtcatcac tttcagggac gattccttgt gctggtaaac ttggcgcgat
     4261 gatttgatcg aatggatcaa tcaattcacg tccattgtca agtagagcgt tcactcgttc
     4321 tctggcgttt aattctacaa aactattttt catctgaaag ttcctccatt gcttgcgtca
     4381 atcgtaggta cacaactcct ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa
     4441 ttggataacg atcaaaaaat ctcgttaata cattgttcca tacttcgccg aagccatcgc
     4501 ttcctgtaca aatttttaca gtagattctg ttttttctgt tggaaacatc aagatttcca
     4561 aatctcctga accgaccact cctacatgga ttttcttatt gatcggctgt ttcgttggat
     4621 atttataagc taattgttcc atggatctct cccctttttg taaaacagag catacacgtt
     4681 ctgcttattc gtctttcttc ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca
     4741 attttatgac caagttctga atttcattgg tggttcatac aggccatctg accattcgat
     4801 caaatcatcg atggtttgtg cagctaataa tgaacgtttc gcttcactac gtttgatgcc
     4861 tagatcttct ggtaatttca cgatgccggc tgcacgcaag ctatctagtg attctttcgt
     4921 gctagtcatt ccaagaggtg taacaccgcc aattgctgca attgctgctt gtcgttcttc
     4981 ttttgttttt gctttgtaca aataagcaat tccttcttct gtcacgatat gtgtagtatc
     5041 ttcgctataa atcatcactg gtgcattggc taagcctgct tgtttttgaa cagctagtgc
     5101 atctaattct tcgacaaaga ctggtttttt atttgaacca tatgtttcaa ccatttgaac
     5161 aactaatttt tggcctttcc ctaatggatc atcattgtcg cgaagtgatt gccatgctgg
     5221 tgtagaatga cgacgaccgc caggattatg tcccatgtta ggcgcaccgc caaaaccaga
     5281 taaacgtcct tttgttacgg tagaagagtt tccttcgtaa tctaattgca aggttgaacc
     5341 gataaacata tcgatggcat attgtcctgc agcttgtgat aatgttcggt tactgcgcat
     5401 cgtgccgtct ttaccgacaa aaaagacatc tgggcgagca gcaatatatt tttccatacc
     5461 aacttcgcca ccaaaactat ggatgctttc aacccaacct gattcgatgg ctggaatcat
     5521 cgttggatgt gggttcaata cccagttctt cgcgattttc ccttttaacc ctagtgattc
     5581 accataagtt ggcagtaaca attcgatagc tgctgtattg aatccaatac cgtgattcaa
     5641 agattgtacg ccgtgttctg cgtagattcc tttgattgcc atcattgcca ttagaatttg
     5701 caattctgtg atgttttgag gatctcgtgt gaataaagct tctaattgat aaggtttatc
     5761 tgctttcacg acaacgtcta cccagtcacc agggatatct acacgtggta aagaatcgac
     5821 aacttcgtta acttgaacga tcacgatacc atctttgaaa gctgcagctt cgatgattgt
     5881 tggtgtttct tccgtattga aacctgtgta caagttgccg tctttatctg ctttatctgc
     5941 agctactaaa acaacatttg gaatcaaatc gatgaataag cgtccataaa gttccaaata
     6001 agtatggata tcacctaatt tcattgtgcc gtcttcaatc atttgggcga cacgcaagct
     6061 ttgtgcacca gcataagaga aatcgatctt gcttgcgatt cctttttcaa aaatattcaa
     6121 atgttctggt cgtgagatac tagacatgat caaatgcaga tcattcactt tttctgcatc
     6181 tactttttca agactttctg aaagaaaact tgcttgtttt tgattgtcgc cttctaatac
     6241 aactttgtcg ccgggagcaa tcagtttttc cattacttct acgatttttt cagtttctac
     6301 atacttgcca ttgagcaagt tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt
     6361 agaagcccag ttgcggtttg gtttttccat aattagtcat ctccattttt gatatagaaa
     6421 cgtggcgtca tcaataccat gttggtctag attgatcgta cggatccctt tatctacacc
     6481 aaattttgta tgaatcagct ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc
     6541 cattcctaat tcttttgcca agtccatcat ttggttccac tgcacaggga aagctaggtt
     6601 ttccaacaaa tctttacgaa tctcttctgc gtcctgagtg gctcttccat ccgtattttt
     6661 tatatacaaa catctagcat cagatagatg gacagactgc atatacggct tgaatttctt
     6721 gaccgtatca ttcatcaaga gacagtgaga tggcacaggg actttcagca gcttcgctgt
     6781 ttgcgcttgg tgccgtttgg cttctgccaa cgtaacggat aaaccttcga tactcccaga
     6841 taacgtatgt tgcatcggac aattttcatt tgacagataa actggatggt caggatcaaa
     6901 tgtttttgct acaatttcct cagcttcgct tctcgtcaca cctactacta cgcccattgc
     6961 ataaccgctt ggataagctt ctttcattaa gactgcgcgg tgatagacta gttccgctgc
     7021 attctcaaat ctcaaagatc gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc
     7081 tgctgcaata tcaggctgaa taccgttttt ttccatttca tctaaataaa atactgcttt
     7141 tgtcagtaat gctagctgga taaaaaccgt atcttgatag ttttcttctt tatcaactaa
     7201 ccgatacccc gtcacatttt ctaaacgttc catgtacatt tcagggatat ccgctaacat
     7261 ccctaacttc tgactgcctt gtccaggaaa taagaaacct gtactcatga gcctcgcctc
     7321 ctcagcactt actttttcca ctgctatctt agtcaatttc tgctataata aaaaatgcca
     7381 aaaaaagaaa aaaccataac aaaaaggtat ggatgggatg gataattatg aaattgcttc
     7441 atctgcagta ttttaaaaaa gtattcgaaa caaaaaatgt tacccaagca gccaaagaat
     7501 tatttatttc tcaacctgcc ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg
     7561 tccctttgtt ttaccatagc ggcagaaaca ttgagacgac cgtttatgca gaagaatttt
     7621 atccatatgc agtcaagaca ttagacacat tagaagaagg aatcaaatta ttgtcttcgg
     7681 tcaatgagaa gatcgttact tccgtcatat tgtatttaga ggtagcctct gtttctattc
     7741 cgaacttggt ccgaattttt atggaaaagc atcctgatat ccaattaacg atcatgcaac
     7801 atcatttacc agatgatatt accgatcctg tactgtacat cacttcggag atgaagcctg
     7861 gcatgaccaa tattcctatc atgaaagaac ctgtttatgt cgccattcct aagaaacatc
     7921 ctcttgctaa aaaagaacat ctgcaattag aagatatcca acgcaccccc atcttgatgc
     7981 tgtcaaagag caacgccttc cgtcatacac tggatgcagc acttgaagcg aaaagtatcg
     8041 aattgacgat cagttcgacg acagatgatc ctgcgacatt acgttctatt gtcaagcaag
     8101 gattagctgt tagctttttc ccaaaagttt cttggtctta cgataaaaat gatcctttcg
     8161 tcttgcggga aatcaaagat ttgcccttga cacgaacaat ttatttatct tctagcttca
     8221 cagaagatca tcctttgatc aaaatgattg caaaaacgat gcgggaattt tatttaggaa
     8281 aataaaaaaa aaagagctgg gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt
     8341 gttccagaag taatttctta gaaaataagc cgaaatttcc caaaaatttg agaagcaatt
     8401 ttcgtaaatt ccttcttatt tcttgaagct aaacatttct gtcacaacct cttctctatt
     8461 gtcctagaca ttcattgcgt tgatttaaaa cttccatcaa tcaaatctgc tagttcatct
     8521 agcttttcta ttttacgtag atcaatagag atccaatgtt ctttattcat gtgataggct
     8581 ggaaaatacc cctcttttcc tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca
     8641 agaatattga tgatcctatg gtcacttgca tcatcataga ttttttcttc tggaacagac
     8701 atcaggaccc caaaccattt tttgcttttt tgatgtctga atacgcaata atcaggaaat
     8761 tttttaaaaa tatattctgg agacaaattg tatgtttcat tgatgtgatc gattagttct
     8821 tttttcgtta gcatctgctt ccatctcttt cttattgatt cgctttattc aaagatacca
     8881 cgtaagaata gaaaaatgaa tacaagtagt tgctaatcaa atacttcgat atttttcttt
     8941 tttatcaact cttaaatgtt caatccgcct gtgttggtct tgctaccatc tactattaaa
     9001 atgtttatta ttgtttgccc acctattttt tgattttagt ggtatcattt ttcaagttca
     9061 tgatgaaatt cttatcatca gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag
     9121 ttgaaacttg accaccgttt attttctccg tattcttaga atagaaggga gtctgattga
     9181 tgtattcact aatgaaaaaa atcattaccg aaaaagatat tcgacgtcat gtttctttag
     9241 tagaacaatt gctgaatcat acaaagataa ccgtaaatga attagctgaa atcataggga
     9301 caaccgaacg aacgattttt tctgaccttc aatctattcg ctctcagctg cccgaaggct
     9361 gggacatcat cagcgatcaa gcaggaatca gtttgcaaaa ccagcaaaat ttattaacaa
     9421 atgacctttg ggagattttt ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc
     9481 tttttacaaa aaaagttgct gtccctgatt ttctagccga ccatgggctt tcttatggga
     9541 cattgaaaag acatgtgact aaaatcaatc agcgtttagc aagttacgac ttgcagatcg
     9601 atttgacgaa atatacagct tgtattcttg gaaaagagcg tgcgattcgt actttttatc
     9661 atcgattgct gattcctttc actcataaca actatttttt cgaggattat tcgatccatg
     9721 aatcccatta ttttcaattt ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag
     9781 aagagatttt cggaacatgt tggttcttta tcaatacgat ccgtataaaa gcgaattgcc
     9841 gattagattc ttctatccat accaacagca cactctcctc cctgtatgac tctgcattga
     9901 aaaaattgta tttaacggaa ggtatttatc ttaaagacac tgagctttct tttgcttctt
     9961 tctgctttct ggaaagttgg aattacaaca ataactatgg acaagaaacc gctcgctgcc
    10021 tgcatcattc tccattttta gaggtcctcg aaacgtttgt agaggaacta gcttcagagc
    10081 tgtctcttga tcaactaaaa aaaacatctc tgactgataa cttggctctc ctcatattaa
    10141 aatatcatga atcgccgata ttgattgaag cattggaccg tcaataccat cgttttttag
    10201 aaacatatga tcagcaatat gcacacttgt atcaacagaa agagaattta ctgaacgatc
    10261 taaaaaaaga aatagaaatc aatgaaccaa attactttct ccaactcttg tctctactga
    10321 tccaacaaac gattttttct atcaaaccga atttgttcaa tgtctatttt attttccaag
    10381 gagaaccttc atggaaggcg ttcttgcagc aggaattaaa agattactta ggaaaacgtg
    10441 tctgcttctt acctatcgaa ctaaccgatc tttccactat ttcttttgaa aaaacagata
    10501 tcttgatttc caatttccct ctagattctc tagatatccc tattgtatac atttcttcta
    10561 ttccaacgaa aaatgagctc aatcgtttga ctgaactcac tttcaaaact tttttatagt
    10621 aacggctagc gactgtattc tctagctgtt ttttcttttt tgcgaaacag tttgttcatg
    10681 atcccttttt ctatttctac aactaagaat agaagaatca catttgctcc aatcaataac
    10741 tgttgtgtca tcgttaaact tactgtaccg atcagctgct ggccgactgg tagaaaaata
    10801 accatccctt gcaataaagc aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa
    10861 agaccttggt tcaatgacgg cttggacaac tctctgcaat tgatcatgta aacagcttga
    10921 gctaagacga tattttgcag aagaatggtt tgctgtaagc tttgcccttc aaactgcata
    10981 gccaataaat aggcaggaac catgatcagt agggagacat agatgatccg aaacaaatga
    11041 taaccagata ggattccttg ctttggatct cttggtgggc gcttcatgat ttctggatct
    11101 ggcgactcga accctagtgc ataagataaa gtgattgtcg ttaccatatt gacccataaa
    11161 atctgtactg gggacaaagg tagcgggcga ttcagcataa gtgcccaaat caagatcaat
    11221 ccttgagcta atgcagttgg caagaagaaa ttgatggttt tttgcaaatt atccaaaatc
    11281 cgccggccct cttttacggc ttttgcaatc gtatggaaat tatcatctcc taaaaccata
    11341 tcagctgcct gtttgcttac ttcactacct ttgattccca tagcaattcc tacatcggat
    11401 cttttcaaag caggtgcatc atttacgccg tctccggtca ttccgacgat ttctccattt
    11461 ttctgcaaag ctttaacaat tcgcagttta tgttctggtg tcgttcgagc aaagacatcg
    11521 actattggca cttgctgaat caactcttca tcagacataa gatccaaatc gatcccttct
    11581 aaaactttgg cagtatgttt taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg
    11641 tgatcccctg taatcatttt cactgagatc cctgcttgtt ggcattcctt tacagctttg
    11701 actgcacttt cttttggcgg atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt
    11761 aaagtctcat gcgtgagttc ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca
    11821 cgctgacctt ttctcgcaaa ttctgcagct gtctgactcc atgcttcttg ttctgcagaa
    11881 gaaagagttg atttagcaaa taatacttct ggtgcacctt tgacgtaaat caccgctcct
    11941 ttttcctccg catgcatcgt tgccatatat ttgtattcag aactgaacgg aatctttgct
    12001 tctatttttt tcaaaggtag attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc
    12061 ggattgccac ttaaattttc tgttttctgt tcttcttttg tttttaattc ctgacagttt
    12121 ttcataatgg acaacacgca ggcttcttct tttgcagcta catccacaac tgtcatttca
    12181 ttttttgtca atgttcctgt cttatctgaa cagatcactg tcatagaacc taatgtttct
    12241 accgaaggaa gtcctttgat gatcgccttt tcttttgcca tttcatgtac tccaacggat
    12301 agaatcatgg tcaaaacagc tggcaatcct tcgggtacca ttgacaccac caaagcaatc
    12361 gtagccgaaa gaaggatatg ccaatccata ccatatcgaa atgtcgtaaa aataactaag
    12421 aataggctca aacaaagaat gccttgaaaa atttgtttgt ttaatcggtt gattttttta
    12481 atcagaggcg tcgtttgttg ttcgaccgat tgcaatgctt gattgatttt accgatttct
    12541 gtcgtgtttc ctgtttctac aacgacaccg actgcagaac cagattgtac aagcgttcct
    12601 gaaaaaccca tatttttctg gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt
    12661 ttttcagatg cttctgattc tcccgtcaaa atcgattctt ccactaataa atcatgtgct
    12721 tcgattaatc ggatatctgc tggaacgaca tctccggcac gtaagacaat gacatctcct
    12781 tttaccagtt ctttcgcagg gattttttct tgcatcccgt ttgatagtac tatcgcttct
    12841 tgtcccatca gctgcttcaa accattcaat gattcttccg cttttctttc ttgccagtaa
    12901 ctaacgagtc cattgacgat cactacgaat aaaataatac ttccttcaat atattcacta
    12961 gaagcaaatt tcaaaaaaga ggcaatgatc aacacgatca tcagcagatc agtaaaatgc
    13021 tttgctattt ttttccatcg ttttgtctgc tgccttgctt ttatttcatt atatccttga
    13081 tcagtcaggc gtttctgtcg ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt
    13141 ttcaatacat cttctaccgt caatctgtac cagttcatcc tatcccctcc tgtttaattg
    13201 atactgttat tatacgcagg gaagtttttt caggcgtatc gattatttca ggggggtctg
    13261 aaataattcg ttttgcaaaa tgtatctggg cctaactgat atttctattg cttattcttt
    13321 tatatacaaa atttaagaat acaaaccaga taaccaataa aaaaggaata aagcgtgtcg
    13381 tgctttcgaa taacaagctg aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt
    13441 tatatctttg tgtagtgttc cgtttctttt ctttaataaa ttgaacatgt gcacacaaga
    13501 tcgaagacca cacaaataga aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca
    13561 atcgactatc agctacaaaa aaatgtagac aaagaccggc aaaaaataac attcctgtaa
    13621 ttaaaatacc gtgtaaaggt acgcctttct tagaaagcgc acaaaaccaa tggggcaaac
    13681 ttttttcttt tgctgtttga tataaaatcc tgctagtact gtagactgca ctatttgctg
    13741 aggacaaaga agcaaccaaa atcattagat tgacaattgc tgctccgcct tggcaatgga
    13801 tcacttgcag catagtcact aaaggacttt cttttaaatc taaacaatgc catggaacaa
    13861 caaataaaat cgacaaaatc gttcctatat agaaaaagag aatacgccaa ggcagacagt
    13921 tgattgcttt tttcaaattc ttttgcggat cacttgtctc gccaacggtt aagccgatca
    13981 tttcaattcc agcaaaactg aataaaacca taggaaatga gactaccatt ccagcaaagc
    14041 caaacggtag taattcatcg atctgtccaa aattttcttt gattccgata gactgggtat
    14101 gaaaatctga gacactcaat cctataccca ataagataaa aaggaaaatc cccaagatct
    14161 tgatgattga gaaaaaagat tccaattcac caaaccactt gactgcaaca aggttgactg
    14221 ccattaagat cattagtgcg ctacctccgc ttatcaaagc tggtacatgt acaaaccaga
    14281 aatggatata caatcctatt gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc
    14341 aataagtcca attgacaaaa aatgcccact ttttgcctaa ataatagtca acgaaatcac
    14401 tgatagagtt aaatgtacta tttgctaaaa ataaatcacc catggcccgc atcaaaccat
    14461 aaatagccaa accgcatatc aaataaacga gaataacagc tggccctgct gatgcaattg
    14521 cctttgctga acctaaaaat aatcctgttc caattgcgcc acctaatgcg attaactgga
    14581 tatttcgatt agttagagtc cgtttcattg ccggtttttc catcaagttc acctttttct
    14641 ttttcaagca gcatggcgac ttcctgttcc caagaaggtt tctttatttt ttcaatacag
    14701 agtggtacta caaacaaaat gacactgatg gctgtgactg caattagatt gatccatgtt
    14761 ccagctttca tcgtcagtcc gacagaaaat ccgattgccg caaataaaac aataacgaca
    14821 gtccaaactc caagattgct ctttttctct ttttttccaa ttctaaatgg tcgattcaac
    14881 tttgcatgtg tataacgcaa tctcaaaact gcgatcccca tgatgacata gacgatacag
    14941 tagataacag tagttgaatt tacgagcatc aaaaatgctt gattgacgcc aggaatcagc
    15001 aaataaaaaa gtgcaaagat tgagataatg acagactgag tcaaaataac ggatttggat
    15061 acgtcaaatt gattcgtctt ccaaaaattg aattttggtg gatacgcacc ttttctagca
    15121 ctttctgtca ctgtctttgc tggtcctgag gcccaagcag ataattggac tgctacacca
    15181 atgaagacaa gtaaactaaa cagattcaca ataagatgtg gcaaaccaag aatttggcaa
    15241 taaatcgaaa tagattgcgc gatattattc aattccattt gtccttttgg tacgacattt
    15301 gctacaacta aagcattcaa aatattgacg aaaaatataa aaattaacgc ggcaaatact
    15361 ccgcgaatat acgttttaac tggattttcc aatcgagtga tatacacaga ggacatttcg
    15421 ataccagtaa aaataaacat gatcggcgca aaataaacaa atgaggatgc agtcgtagta
    15481 tctggtatta atttgtccca tgaaaaactg cctaatgttc cggttggtac gatcccaact
    15541 ttgatccacg cagcaaaccc taataagagc atcatcgtca aaggcacgta caatcctaac
    15601 caaacaccga cttttccgcc gatttttgcc atatcaaatt tcaaattcaa aaaagtgatg
    15661 atccaataaa cgactaaaat gacaactaat gtaaatttgc tgttcaaacc aagcggcaca
    15721 ttatcgataa tattaccaaa caacggagcg agtgctgaag cgaccatcac catcccagga
    15781 aacatttgga cccataacag ccatgagaca acgaaccccc atttttttcc tagagcattc
    15841 gatacccaaa gttctggacc acctttttgg ggaaacattc cagcaaattc accagagatc
    15901 aatgagattg gaaaagcgta caataatact gcaaccagca tgtaaaaaat catcgtccag
    15961 ctcgttgctg ctacatctgg aatatttcgt acacttgcta caagggcgca ggtcattccg
    16021 ataaaggtca tggtcgtgat ttgctttgat gaaccagtat ctttcatctt tattccttct
    16081 ttcaatagcg acatatgttt ttgttttctt ttatttttgt tcaagaacag aaatgacaag
    16141 gtttcatcga ttcagtacaa aacccagtat caccgaaaca tccattgaac gatcttagtg
    16201 aactgggttt gatacatgaa acgaacgatc caacttatag aacgttttat aaaaaagaat
    16261 atcttggcga ggaatatatg aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata
    16321 attgtctgtt ctccatatgt ctcttgttat tctttcacac aaaaatcagt tctattggat
    16381 gatttcgttt agtttctttt ccattgcttt ggcgattttt tttgtataaa aattgaaaat
    16441 tcgatcatca ttcaaataag gagtcatgat agccgcacgt agcagagtga ttttctgttc
    16501 ttttttccat tcttctttcg ttagacccat tctttcaaca aaacgaatcg gagaatcacc
    16561 gtaatcttct tgtgtaaatg tagtatgaga agtaataaac cgttcaccat aaacatcgcc
    16621 atccatataa gaagaccgat cgaacatttt ttcatttaat tcattgatag cgttcaaatc
    16681 tgtacagcct tgttctttga atacccaatt gaccatatta aaatcaggat gattcaatgg
    16741 atagacttcg attgtttttc ctttaacagt aaaagtcaga tgatcgagaa attctctgaa
    16801 tctttgagct gcctcgattg aagccccaat caattgacca tatccggtca cattcaatgg
    16861 caatacacga tgtgctgtcc agactgcggc ggcggttgct ccagcttttg atccttccaa
    16921 aatataagct cctaacatat ctggagcctt tactgatttt tcaaacacat atggcgcgaa
    16981 ataagaaatg atatttctca tatttttatg tttgattacg attcctccag cagcataagg
    17041 gacatagccc atcttatgag gatctatcgt gacagaatcc gcttctgaaa tagctctgaa
    17101 cccttcatat acctctttgt caatcgttac gcagtggtgg aaaacatgat gttcttcaaa
    17161 gaattcagct aaggaagcat acggcacaaa ttctcctgct tcgtttagaa acagtgaacg
    17221 cgcatagccg ccatatgcgg catcaacatg taaatagaaa tagatccctt catcttttaa
    17281 cctttctctt aattggacga ttttatctac actgtctact tgaccttctt ctgtcgttcc
    17341 tacgacagca accacaccta gaattgggat tttttgtcca gctaattcac gaattgtttt
    17401 ttccaaagca ttaatatcca ttcgataatc ctcttgaact gggatagcaa ccatctgatc
    17461 taagccgact ccgcaaatat ctaacgcctt catccaagaa tagtgttttg tttgagggac
    17521 cagccatttc cctaatcttt ggatattttt tccgctacgg gaagaagctg ctttgacctc
    17581 atccatttct tcgtccgtaa agctttcggt catttccaaa atttcttcaa cagataaatt
    17641 caaaagttcc cactcagaca tttttttcac tttttcaggc agcacttcct tgacagctaa
    17701 aggaatcgat ttgatgcaac gggcgtacca taatccttca agattagcaa tagaaccatc
    17761 tgctgcaatg tgtccccatc cgtctgtcat attgaataat gaagcaaaat cctgtcctac
    17821 ttctgcttcc atttgcgaag ttgccataga agattccaat gcaacattat ttgggttcca
    17881 cagcatcgca tagttgtaag ctaataaagc aggcatcaat gtttcagcat tcatttgtcc
    17941 ccagtaacgg cctgctgaat gccatgggat cgatcctgcg cgtaatcgct gactgacttc
    18001 atccaagacc tctatcatat ggtcgcgggt ggctagataa tctggagtga gcttatctcc
    18061 ttcgctaatt gcaggcatat ctgaaggaat ataattttct ctccatccaa gatgttcatc
    18121 taccatcttg ttcaacaaca ttttataaac tggtccattt tcagctttgt cgcctataaa
    18181 gacggccttg atatccatat ctttcatttt tcttccctcc atattttgtt ggtcaatgtc
    18241 ttgcagatac atgtgtttat ctgtaacacc tcttcactat agcgccaaaa atgagaattt
    18301 tttttttata acatcaaatc cacgtagatt tttacaaaca aaaataaata aagtcggatt
    18361 tttctgataa ttcaacgcaa aaaagcgaac aatttttcaa taaatcagtc ttctaaaata
    18421 gataacctaa aaaaagcgaa caaacaaata aaatgactgt ccttcattca ctaaacatat
    18481 ttttattttt ttatattgtt aaaaattcgc aatttatttt ttaatagaat tatctgtttt
    18541 ccaaatttta taacataaaa aacctttttt aacggcattt atatgctttt tcagaaatta
    18601 actgatgatt tcaaaaagaa aacatgtcat aatatttccc atattgtgca aaaaaataca
    18661 aggcggaggc tctactatga ttgaagaata tattgagaaa aatattttaa gacagctgtt
    18721 tttatgtggg caattctatg taaataaaga agtcaatcta gaaaagctct ccaatctcct
    18781 gcatgtttgc aaaaccactt tactcaatga tatcaataac ataaaaaaag aatttgaaga
    18841 acagatcgct tatacacatc gggaaaagga ccgttacact ttatattttt cagaacatat
    18901 acctcgatgc aagatcatgc agcaattgtc tcaaaattcg ttgtttttaa aaacttgtct
    18961 gctttatttg gaagaagatg aacctgatta tcttcaatta actgaatgtg aattcatctc
    19021 tgtcagcaaa gcttattcat taaaaaaaca agtattagct tattttaatg actgcgggat
    19081 cgaaatcgac cgctactccc ctcgattcac tgaaatggaa agacgtctgc tgcttcttaa
    19141 tgtgtcgtat cgtttaggcg gttttaattc ttgggagcta cctgaatcgt tttttgaacg
    19201 tgccgatcgt ttcattgaat ccgtaacgga aaatagtggc agattttatg ataaagaaaa
    19261 taaagaaatc ttatcgattg gttttgctat cagtttccta cgccagcaag tgtgtgctgt
    19321 gacaatagat tcaaaattta tcgaagaaat caaaaaacgt cctatttaca attatgtcga
    19381 atcagcatgg gaaaacactg atttccaaac ctattataaa aaagaagaat tcgcctttat
    19441 tttgacacta ttcaatcttt gtaattatgg tttccattct tatcagttga ttgcggaaga
    19501 ctttcaacag ctacaccaag tgtttattga caatactccc gaaataaaag aactagttgc
    19561 tacctttgaa tctcacttca atcaagaatt attcggaaac caaccgttcg aacgtgcgct
    19621 gattcatctg atgcgttcgg cttgggataa ctatcaatta ttcatgccag aaaaatttta
    19681 tcttttaaat gaggaacaga caaatttata taaagaagtc cagacaattt tttccagctg
    19741 gtccagccag ttaccttatg atctacggtt gaatcctaat tgcatgcggg cttttgtgat
    19801 cgaattgagc ggaattctcc gtctgacaaa agaacatctg actatctata tcgtcacaaa
    19861 ttcggatgtt cattatctga tttatcggga agccttggaa gctgtaacta ctttcgattt
    19921 tcaagttgca ccaacaattt attcaagtat atctgatata aagaagtatg cgcagcagtc
    19981 ctcaaaccgg gtattatgtg aacgtacatt atatacacca gatgcggtcc aatatgaaaa
    20041 tattattcct atttcgatca atacgatcga tcgagctatc atttcagctg tgcaaaacaa
    20101 ataaggggct gtgacaaaaa tcatgtcaca gcctcttatt tgttgaagat gcccatttct
    20161 gtaacaatct cttgatacac gtcaggctca caacctcact tattttggac atctacgggt
    20221 ccgatctctt tatttaaaaa ataagaaatg atggttcgga taactaccaa cgccgctagt
    20281 tggaaaatat cctgcaaggt aggtttgata atcgattcga tgatcccagc tgaaacaaga
    20341 atttccaaac ttagaagtat atagcctccc aacatctttt taatagcatt gttctcgctg
    20401 actcggtcgc tttgcacttt tgtatggaca aataaatatt tcaaaaaatc cttgattgat
    20461 agacaaacac cccaaaccaa taaacagata gcaaagatat caagtcccag tacaagtttt
    20521 tcgaaatatg gaagaagaaa ctgcatcaac tctgtcgtca tcctacctct cctcttcttt
    20581 gtttttcttt gtcttctatt tcctgattca agaaataaga aatgactgtt cgaatgataa
    20641 taataagtgc aagcttcaaa atatctgtcc aagtcggctt gataactgac tcgatgatat
    20701 cagcaacgac tagtacttcc aagcttaaca aaatataact tgctagcagt tttcttgtat
    20761 ctgcattacg agtattacgt tctgcatagt ttttcttgac atgagaccaa tcaaaaagtt
    20821 tgaatagaac aagaacgatc ccgacaatta agataccgat agaacaaata tttaaaatag
    20881 caatgatcca ttctagaaat tcaattaaat ttgagcgaaa catatacgct acctactttc
    20941 ttttaatttg ttcctattct tttattgtaa aagaaagtta ccttgttcgt ctatttaaaa
    21001 gttgttctaa tgataacggt tcgcttaaat ttttcttttt cactctccca tttttctccc
    21061 tccatccata aatcgtggaa gtatccaaaa caacgtagca agttaaaata aggcttagtc
    21121 cgtcatcagc ttttcaaaga agcactgtct cggtgctttt tttgtaccca aatttctaaa
    21181 aatagaacca tacacctcct caaatctttt cttcttacct accctatttt tatttacagg
    21241 aaaatcagac acttcttctt ttttgtcaaa atttcctttt tcttctcgtc ctatcatttg
    21301 gcattctaat aaaaacgaac tatccttgga tatagaaaga gaaaacaagg aggcgttttt
    21361 catgagtaaa aaattaatca aagtcggcat cggtttaggt ttgcttgccc ttggcgctgc
    21421 atatctagga aaaaagacag gactattcga agatgacagt catttatatg atgaattcga
    21481 atcaatctag catcctattt agttgaatca ctacggacat acttggaaga cattggagga
    21541 atcacaatga gaaaaagtaa agcaataatg attggtgccg gtattgccaa tatggcagcg
    21601 gctgtttatt tgattcaaga aggcaaatgg aaaggggatc agatcacctt ttattcattg
    21661 gatgatcacg gttcaaatga tggtgcaccg accgacactg tagtggatga atattggaat
    21721 aaaaaccatc cattagaaaa ccaaaaaggc tatattgctc gaggcggtcg catgcttaat
    21781 taccgtacct atgtagattt gatggatctg ttaagtcgaa taccttctgt tactgagcca
    21841 gggatgactg ctgaagaaga tacgagagat tttgatgcaa aacaccgaac ctttgacaaa
    21901 gcacgtttga tggaaggtgg caaaggaatc attgatggcg gaaaactggg attcaataat
    21961 aaagaccgta ttcttctgac taaactgatt gctatgcctg attcagaaga agaaaaacta
    22021 gacaatatca ctatcgaaga atatttcaaa gatgatccac acttttttga aacaaacttc
    22081 tggtttatgt gggaaacaac atttgctttc agaacacgta gttctgctca agaattgcgt
    22141 cgctatatgc atcaaatgat ttatgaattc actcaaatcg aacatctagt gggcgtcaat
    22201 cgaacacgtt acaatcaata cgaaagtatt atgcttcctt tgatcgagta cttgaaagaa
    22261 caaggatgca aaatcatttt gaaccgccgt gtcactgact gggaattcaa agaaacaccg
    22321 atgcaagatg aaatcactgt gactggtttg aaaattacca atacactgac tcaagaagaa
    22381 gaatacgttg tggtcgatga agatacagca gttattttca ctaatgggtc cattacagat
    22441 tcagctactt taggagatta cgaaacacca gctgtagaaa atatggatta tggtgcagct
    22501 tctagtctct ggaaaaaagc cagtgaacgt ttctacaact tgggaaatcc tgacaaattc
    22561 tttgctgatc gagatgcaag cgaatgggtc agctttaccc tgacgacgaa aaaccatttg
    22621 ttgttgaatg aaatcacccg tatcacgact caagaaccag gaaatgcctt gaattcattt
    22681 atttcaacca caccgatcac gccgttaggc caaaaagatg tcaatatgtc tattgtcgtg
    22741 catcatcaac cgcattttac gtctcagaaa ccaaatgaaa cagttatttg gggttatttc
    22801 ctttatccaa gacgacgtgg cgaatttgtg gataaacagt atattaaaat gaatggaaaa
    22861 gaaatgctag aagaactgat tggacaactt tcaaaagtag atcctggacc agtgaatatt
    22921 cgtgaaaaag aaacagaaat tttcgacagc gtaataaata atatccctgt ctatatgcct
    22981 tatgcttccg cgttgttcaa taatcgtgcc aaaagcgatc gaccaaaagt cattcctaaa
    23041 cattcgacca atctggcctt tacaggagaa tttgtcgagc agccttatca gatgatcttt
    23101 actgaacaaa gtgctgtacg ttctggagaa atcgctgctt tccattttgc gggtattcct
    23161 atgtcccgtc ttgtcaaaac accgcgttac gacaaagata ttcctactct agctcgtgcc
    23221 gcaaaaaaaa tgtttgaata atcaagaggt tgtgaaaaag ctgccctgca tcaagtaata
    23281 agaacaacca ggctaaaaca gcttcttatg tttttgattt ggttggactt attaccgcag
    23341 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aagctgccct gcatcaagta
    23401 ataagagcag ccaagctaaa acagcttctt atgtttttga cttggttaga cttattaccg
    23461 cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaaggcgt ttagtttcga
    23521 gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt tgcataaatt tgacttaata
    23581 ccgagaaact gatcccttct gtcataacct ctttttattt atgtctgttt ctttttgaaa
    23641 acaactattt tttcaacgtt tactctcttg ccaatccaac atcatctaac ggatacaact
    23701 atttcaatga ttaccattac actaaaaaat cggaaacaca cctctccgaa cacaaataca
    23761 taagactata gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc
    23821 tcctttaaaa tgaaattatc tgtagtacta ataatgcaga caattttatg aatctgaaag
    23881 gagcatctcc aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg
    23941 tagatgatcc aaaaatatta gaaccaacag atgcaatcat ccgtatcaca gcaacagcca
    24001 tttgcggctc cgatctgcat ctttaccatc atggtgaagc agttttggaa aaagggtatg
    24061 ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt caaacattga
    24121 aaaaagggca acgtgtggtt atccctttta atattagttg tggtcattgt catttctgcc
    24181 tgaatcacat ggaaagccaa tgtgatgaat caaatccaga tcagttatat ggcgggcttt
    24241 tcggatttgg caagttgaac ggtaatcatt ggggtggtca agcggaatat ctaagagttc
    24301 cttatgcaga ctctacttca tttgttgtac cagataatga tcttcctgat gaaaaagttt
    24361 tatttctatc agatattttg cccacagctt attggagcgt tgaaaacgca ggagtaaaaa
    24421 aaggcgatac agttgtgatt ctaggttctg gtccagtcgg tctattcgct caaaaattcg
    24481 ctgttatggc tggagcagag cgcgtaatcg cagtagatcc agtacagcac cgtctagata
    24541 aagcggctaa ttacaatggg gttgaaactt atttcttaga agacacggca caagctggaa
    24601 cagaattgta tgaactggct aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg
    24661 acggattaga gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa agcggcacaa
    24721 tctctccatt acagatggca tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg
    24781 gtgtttatat gactccagct tcttcttacc cgctgcaaga attttttatg cgggatatcg
    24841 ctgtcaagca tggacaggca ccaattatcc atctaatgcc aaaaatttac gcaatgattg
    24901 cagaaggact attcgatcct acacaaatca ttactcattc gctgccacta tcagaagcag
    24961 cacaagcata tgagatcttt gacaaaaaag aagataaaaa tataaaagtt gttttgaaac
    25021 cttgaattca gtaaaaaata caggtgattt tctttatgcc caaaatcaaa cgaaataccc
    25081 atgagatcga aataggaaaa gaagaagata tcgaaatacg aggacgtcgc ttccgcttct
    25141 atttccaaaa tcgttacaca ttgatctcac tggctgtcga tttattaacc gggattttct
    25201 atatttttgg aagccttgct tccttaacta aaatccccga tcgatatggc acctattttt
    25261 atttggctgg tgctattttc ttgacgattc gtcctattct cagaatcgta aggaacattt
    25321 ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat
    25381 ttttcaaatc tcatgcctct tttttcaccc tatcgtatca ctttgataca gacgagcggt
    25441 cgtcccactt tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt
    25501 ggtatgatct gtaattaagt aaagtttttc gtacccaagt tgatgcattt cttttctgat
    25561 ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa caaacagggc
    25621 acaaagattt ggtgtcagat cagggcggtt atggaagtcg ttttcaatga cgcccgctcc
    25681 tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac tttccttatt
    25741 agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc attttgatga
    25801 aaaccaattc gcgctttgtt ttactaattc tgggtgttct cttaatttga tccagttata
    25861 tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt acaaaaatta tacaagtctt
    25921 tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aaaacggaaa acaatcactt
    25981 gttctccgtt ttttgattaa tcaagcatct aaagcttctt ttcttctttc ttggatcgta
    26041 gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac agcggctagt
    26101 acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg agttacccat
    26161 ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta agcaacacta
    26221 acta
//
LOCUS       DACCVU010000031        25834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCVU010000031 DACCVU010000000
VERSION     DACCVU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.031"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            254..808
                     /locus_tag="IVW29_001131"
     CDS             254..808
                     /locus_tag="IVW29_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7838627.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(981..1454)
                     /locus_tag="IVW29_001132"
     CDS             complement(981..1454)
                     /locus_tag="IVW29_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7838628.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1466..2002
                     /locus_tag="IVW29_001133"
     CDS             1466..2002
                     /locus_tag="IVW29_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7838629.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2042..2413
                     /locus_tag="IVW29_001134"
     CDS             2042..2413
                     /locus_tag="IVW29_001134"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7838630.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2697..3968
                     /locus_tag="IVW29_001135"
     CDS             2697..3968
                     /locus_tag="IVW29_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7838631.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4213..5238
                     /locus_tag="IVW29_001136"
     CDS             4213..5238
                     /locus_tag="IVW29_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838632.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5532..6641
                     /locus_tag="IVW29_001137"
     CDS             5532..6641
                     /locus_tag="IVW29_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7838633.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6724..7620
                     /locus_tag="IVW29_001138"
                     /pseudo
     CDS             6724..7620
                     /locus_tag="IVW29_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7636..8460
                     /locus_tag="IVW29_001139"
     CDS             7636..8460
                     /locus_tag="IVW29_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838634.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8492..9802
                     /locus_tag="IVW29_001140"
     CDS             8492..9802
                     /locus_tag="IVW29_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7838635.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9950..11209
                     /locus_tag="IVW29_001141"
     CDS             9950..11209
                     /locus_tag="IVW29_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7838636.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11219..12091
                     /locus_tag="IVW29_001142"
     CDS             11219..12091
                     /locus_tag="IVW29_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838637.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12103..12933
                     /locus_tag="IVW29_001143"
     CDS             12103..12933
                     /locus_tag="IVW29_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838638.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12973..15156
                     /locus_tag="IVW29_001144"
     CDS             12973..15156
                     /locus_tag="IVW29_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7838639.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15217..16437
                     /locus_tag="IVW29_001145"
     CDS             15217..16437
                     /locus_tag="IVW29_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7838640.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16434..17894
                     /gene="gtfA"
                     /locus_tag="IVW29_001146"
     CDS             16434..17894
                     /gene="gtfA"
                     /locus_tag="IVW29_001146"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7838641.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17971..19077
                     /locus_tag="IVW29_001147"
                     /pseudo
     CDS             17971..19077
                     /locus_tag="IVW29_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19399..19608
                     /locus_tag="IVW29_001148"
     CDS             19399..19608
                     /locus_tag="IVW29_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7838642.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19601..19750
                     /locus_tag="IVW29_001149"
     CDS             19601..19750
                     /locus_tag="IVW29_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7838643.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19785..20569
                     /locus_tag="IVW29_001150"
                     /pseudo
     CDS             19785..20569
                     /locus_tag="IVW29_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20853..21938)
                     /gene="xerS"
                     /locus_tag="IVW29_001151"
     CDS             complement(20853..21938)
                     /gene="xerS"
                     /locus_tag="IVW29_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7838644.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22272..22505
                     /locus_tag="IVW29_001152"
     CDS             22272..22505
                     /locus_tag="IVW29_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7838645.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22502..22909
                     /locus_tag="IVW29_001153"
     CDS             22502..22909
                     /locus_tag="IVW29_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7838646.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24046..24564)
                     /locus_tag="IVW29_001154"
     CDS             complement(24046..24564)
                     /locus_tag="IVW29_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7838647.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25682..>25834
                     /locus_tag="IVW29_001155"
     CDS             25682..>25834
                     /locus_tag="IVW29_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7838648.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         8810 a         3948 c         4816 g         8260 t
ORIGIN      
        1 tagctattct aagaacttct acggataact gcccgtctaa aatttaaata agagcttaga
       61 atcttcatat aagtaaggtt aatttttttg tacaaaaaag tcggaatatt ctgagttact
      121 gaaatgatat aaaatcataa actaaatccc ttgattttat tgactttcaa aagtttatat
      181 atataagtta atgattatgg taaaatatat acataatcat taactcagaa tatttttaag
      241 aagggaaaca atagtgagct ataatgtaca accgttaagg acaaatgaag agatacaaga
      301 ttttcagttt tggctaagaa gaacaagtaa tccggaaaga gatagttttt tatttttgtt
      361 tggaatcaat aatgggttac gtatgtcaga tattatagga ttaaaagttg gagatatacg
      421 aggcaaaagt aagcctatta ttgttgaaca taaaacaggt aagcgaaaac cgatttttat
      481 agataatttg agagaagaaa ttttattata tactgaaggt aaagaagaaa atgattggtt
      541 atttccgagt cggcaacaag gtcgacatat aacaagggat cgtgtctatc agatatatgc
      601 agatatagca gaaaaattag gacgagatga tataggaaca catacattaa gaaaaacttt
      661 tgggtatcac tactacaaga aaactagaga cattgctacc ttaatgttta tttttaatca
      721 tagctcgcaa gcaattacaa aaaggtacat tggtataact gaagatgaaa taggagcatc
      781 acttcgtgga tttaaactag gtgtataatt taaaaatttt tataaacagt aaagacaaag
      841 attaaaagct atttttatct agaacgattt tatttcataa agttcgcgga ataaaagctg
      901 gggttactgg tacttggtat aggttcgtgt tatataaact aagtaattaa ataacatttt
      961 taggttggaa aaggatgaaa ttaaaaaaag agaatcaata cggtctcaaa aacgcaaacg
     1021 ctgcggaata agaaaaatac tgataactca acgtttagca gactatctaa tagggatagt
     1081 ctgctttttt cgtttttgac catacttttg accttctgca aacttccttg cttttcctct
     1141 gtagaactca tgaaatctgc aaaacgaatt gccgtttcct ctgtctgctt tttcgcgata
     1201 tgggtgtata tatcatccat agtggtttta atatctgtat gtccaagtcg aacatacact
     1261 tctttgacag acgcaccaga ttcaaagagc agactacaat gggtatgacg aaaaccatga
     1321 agtgtgatat gttttaagtt attctcttta atgatgattt tcaaattgtg atttacatag
     1381 tctaaataaa ggggcttatt ttcttctgat gaaaacaaaa gttgttcacc atcttttata
     1441 ggtttgattc tcatttttag atattatgcc cgcacgtatt catcacattt ctattgtgaa
     1501 tcgctttatt cgaccgacat ttgactttta tcataatata ctcggtttaa aattactaat
     1561 gaaaacaatt aatcaagacg atcataccat gtatcacctg tttttttcag ataatcacca
     1621 ccgtgtggga actgaactga ctttttttga agtacaagaa gggaacaacc agttttttgg
     1681 aaccaatacg attgaacgaa cgatactaaa agttccttcc gaagcatcat tgcatttttg
     1741 ggaaatatat tttgaaacac aaggcgtttg ccattatgga atcgaatcat tttctggacg
     1801 acctatctta cgttttgaag ggccagatgc aacacgatta gctttagttc ccttacgcga
     1861 atttgaagat attgatgatt atttcccgta tgtcacagac cagatcccta cagagcatgc
     1921 cattttagga attgatgcca ttcaactacg cgtacaatat tccgatgcga ctgaacgaga
     1981 gttattgtcg gtaggctggt agcacaaaga atacgtcagt ttcttttcgg gtcatcaacg
     2041 agtgacaatc ttagaaaatc aagacactca gttctatcaa gaagttcaga ttattcaaga
     2101 tcgtgagaat ccagttgcca ttgaagggat tggtagcgtc catcatgttg cgtttggcgt
     2161 agaaaataaa tctgatttac aacgcatcga caaacaatta caagagcgca attttataaa
     2221 ttcaggaata aaagatcgtg aattttttgt atcactttat tatcgtgacc caaatcaatt
     2281 attgattgaa attgctacag gggaaggaaa cctagatgca aaagcgtatg aaaatcaaag
     2341 tccacgcttt gaagaaattc ctttattttt accgcagtac ttaaatttca ttcgtgaaca
     2401 agtggagcaa taattagcta atcaaatcca tgacgaagag taagaggttg actcataagt
     2461 caaaaaataa agaaaaacgc atcaaatcca ttctaaagaa tgttgatttg atgcggttcc
     2521 tatttttata aactatcatt ttcgactaat aaaactactt atgagtcagc ctctcttttt
     2581 ttcttgattt ttatacctct ttctaggtaa actaaacatc tgccaaaaat accgtgtctc
     2641 attgaaacat tctcaactat ttcaaaataa aggggagtac caaatgaacg agtatattga
     2701 aattatccac gcaagagaaa caacttaaaa atgtcagtct caaaattccc aaaaataaaa
     2761 ttagtatttt taccagcgta tctggttcag gaaaatcatc gattgttttt gatactatta
     2821 cccaagaagc tggtcgtcaa ctgaatgaca cctatgatag ttttacccgt ttatttttac
     2881 ctaaatacag tcgtccagag gttgatgaga ttgataatct ttctacggcg attgtggtca
     2941 atcaaaagaa gattggtggc agtgcgcgct caacgttagg gacgattact aacatcaatg
     3001 cgttatttcg tgtgttgttt tcatgttttg caacacctag tttaggatat gcaaatgctt
     3061 attcatttaa tgatcctagt ggaatgtgtt ctgtctgtca gggaatcggt aagacgattg
     3121 catttttaat aggattgctt tatttttcgg ctattgataa tgctgatttt gtattttctg
     3181 atccatttac cgcagaagtt ttgtctggaa ttgaggaaca ggcaaagaat catggttatt
     3241 ttgtattggt gcataatgtt aattcagcag aagatgttaa aactattcag agaaattggc
     3301 gttttgaggg gttcattgtt gttggtgtac gtgtaaaaga atttttggaa ttaaataacg
     3361 tttttgatgg tcctgttgtt tatattgata cgcatctttc aaaagagaca ttaagagaac
     3421 ttgaacaaca aatgaatcgt ttatttgtaa atacaaatga ttaccaagct agtacattgg
     3481 cagtggaaca tttgattcat tatggtcatg agaaaatagc gtttctttct tttgactaca
     3541 ttgtaggtga accaagtgtt attcaagaac gacatcaagc ttatatcgat acattaaaga
     3601 aatatcattt aacctttaaa tcgcattgga tatataacaa ctctgaattt gagcgaattt
     3661 atcaagaatt agctgaattt actgcaattg ttgttactgg agattattta gcggctaaat
     3721 tagtaaaatt tttaaaaaat aagaataaac gagtgatgga acaattatcg attattggat
     3781 ttgatgatat tacttttgca gaattaatgg atcctgcgtt gacaacaata cgactaaatc
     3841 caggtaacaa gggggcgatt gcttttcacc aactgattgg actggccaaa caagaagaaa
     3901 taaaaaatca agtactacta ttagagggag aattgatttc tcgagactct gttcacaaaa
     3961 tcaaataaaa aagctctgtg aaataagtat agttaacaat aaaatatccg aacgatagag
     4021 agaatgaact gtgatctagc agttcctcgt cgcaaattca ctagaattag cattatttac
     4081 tctaaattgt tcggatattt tttcttgttt ttaattatga ttacagtgcc tatttttaac
     4141 gaaatttgct tattttcaaa atgtatgatt atatgttagt aaacactttc aaatatgaaa
     4201 agaggagcta ttatggtagg aattagggat gttgcaaaac gggcgggtgt gtccattgca
     4261 acagtatcgc gcgtgctaaa tgaggatccg acactttcag taaccgatga aaccaaacga
     4321 aagattgcag aatcggttaa tttttatcat tatgtcaaaa aaagtcccgt tgcaaaaaat
     4381 ttaacaaata ttgcattaat aacgacagtt tctgaagtag atgagttgga agatccctat
     4441 tttcgaacga ttcgcagagg gattcaagtc gaagcagaac aaagcaaagt caatttgaag
     4501 aaaattattc gtttgtcaga aacaccgttg gagcaagacg atttgctaaa atgtcaaggt
     4561 gtgttgatta ttgggcaggt gctatcttct gtgattgaag aagtatcagc aattaattcg
     4621 aatgtcgtag tgattgatga ccctagtatt gaagctgaag tagatgcagt ttatactgat
     4681 ttaacaaaag caacgaatca gcatttagat cgattgtata aaaaaggaca ccgaaacatt
     4741 gcgtttatcg gaggaggacg tgttcgttta gatagccaag gaaataaaca cgtgtcagat
     4801 gatgattatc gaaaaatagc ctatgagacg tggatgaaac aaaaaggatt agaaaaatat
     4861 agtcaaattt atttagaagg ctggaccaca cttgacggaa tgcaggcagc agatttactt
     4921 ttgaaaaaac atgctaaaga taagttgccg actgctatca tggtaggaaa tgatcctatt
     4981 gctgtggggg tttatcgttc acttcaaaaa aatggtctac atattccaga agatatttcg
     5041 attgtgagtt ttgataatat tgaagttgct gaatttttaa cacctgcatt aaatacggtg
     5101 aatgtgaata cagaagaaat tggaaaacta gcagttcgca tggttaatga acgaataaaa
     5161 gaagttagaa atgtttcgat caaagtgatt gtttctaata atattattgt tagagaaagt
     5221 gaaaaaaata tcgattaaaa aagtaaacat ccaaaaaagg gtgtttactt ttttgtttac
     5281 taatattttt agtaaaaaat ctcgacaaaa cattaagtaa acagttatgg tgttgttaac
     5341 aaaaagaaaa cgcattcaaa acaggaggaa ttaagatgaa acatttcaaa aaaatagctt
     5401 taacagggat agctgtttta actgggacat tactcgcagc atgtggtggg ggagatagta
     5461 attctaaaga cgggcaagta actttaagct atgcgagttg agatactgac caagctccag
     5521 gactaagaaa aatgttagat gaatttgaaa aacaaaatcc tgatatcaaa gtagaaatgg
     5581 agacaacgcc ttgggatcaa tattgggtta aattagaagc tgcaacaacg ggaggtaata
     5641 tgccagatgt agtaaccatg cactctagcg aatcctataa atatatgtca cagaatgctt
     5701 tgatgggact tgatgatgtt atcaaagaaa atgatattga catgggaaac tttactgaag
     5761 gaattgctga tttctatacg cttgaagacg aactatacgc aatccctaaa gatgcttctg
     5821 tagtggggct atggtacaac aaagaattat ttgataatgc aggtgtcgat tatccagatg
     5881 aaacatggac atgggaaaca ctcagacaag ctgcaattga tttaacagat gaatcaaaag
     5941 gcatctatgg ttttgcatca catattggaa cagagactgg ctactggcca tttatttatc
     6001 aaaatggggg agaagtattt gccgatgaca atacaaaatc aggtttgaat accccagaag
     6061 cacaagaagc tttagctttt tatcgagata ttgcacttgt tgatcatgct tcaccaacaa
     6121 ttacagaatt acaagatcct gataagttaa gtcgttttca atcaggtaga ttagcaatga
     6181 tgattgaagg aaactggttt gcaccgggat ttttgaaaaa tgactatcta gttgaaaatg
     6241 ttgcttctgc cgtgttacct aagggaaaag ttaatgcaac aattacaaat ggacttggtt
     6301 gggctgccag tgcgagtaca aaatacccag aagaagtgaa aaaactaatg gcatttttag
     6361 ctagtgagga cgccaataaa atccaagcgg aaacgggggc atcaatccca gcactaaaag
     6421 gctttgaaga cgagtgggct gcttcttcag atacaattga tttgagtgct tttggaaaaa
     6481 tgttggagta tggagtccct cgtccataca ataaagatgg attacgagct gaacaagcag
     6541 aagttcaaat tattaaccgt attctgtctg gtgaagtaac tgttgaagat ggaaccgctc
     6601 aaattgaaaa agaagtcaat gcgatattga gcggaaatta aattagttaa ttatacaata
     6661 tacaggcgaa aggattctat ttcgtctgta tattaatgga tgaataccaa aaagggggat
     6721 attatgaaaa atcgaatttt tggaaaatat cgtaaagatc aagtgatatg gggctatatt
     6781 tttatttttc caacggcatt aggattaatt ttattaaata tatggccagc aattcaaaca
     6841 gcaattttaa gttttcaaaa aacggtaggg ttcggtgata ctgaatgggt cggattaaga
     6901 aattaccaaa aaatgtttgc agacggtgaa gtttttcagt ctttattaaa cacattaatt
     6961 tatgcagtgg tttcgatacc tagtattgtt gtattatcct tgttggtggc agttttaatg
     7021 aatagaaaag ttaaaggatt gtcgatttat cggacaattt attttctacc agtcgttgct
     7081 gcaccatccg cagtcgcaat gatttggcgc tggatgttca ataatgatta tggactgatt
     7141 aataacatgt tggctaaaat aggtctcgat ggaccagcat ggttaacgga tccatcgatt
     7201 gcgatttatt ctattatcat tgtaggagtt tggagtgcta ttggttataa catggtgtta
     7261 ttactagccg gcttataaga aattcctaaa gattattatg aagcagcgga aattgatggt
     7321 gctagtccaa tccagcagtt tttcaacatt acgttaccat tagttacacc aacattattt
     7381 tttgtagttg taacaacagt tatcaatgct ttccaagtat ttgatgttat ttttatgatg
     7441 attactccta atagtaccgc catgtttaaa tcacaatcat tagcctattt attctataaa
     7501 cattcattta ttttaaatga caagggatat ggttcggcaa tcgttatgtt ccttttagtg
     7561 attatattaa ttattactgc aattcaaatg aagttgcaga aaaagtgggt aaactattaa
     7621 ggaggctatc aaattatgaa aaataaattt ttttcaaatg cgccaatcca tatcatgctt
     7681 attttaggtg gtattgcaat ggcattacct tttatttgga tgattttaac ttcagggaaa
     7741 acgttggttg aatctaccca aattccacct acaattttcc ctgagaaatt tcagctaagt
     7801 aactatcaac aagtacttga tttattacca ttcatggctt tttatatcaa tacattttta
     7861 atggttgttg gacgagtgat tggctcggtt ttattttcag caatggctgc atatgcttgt
     7921 gcgcgtctaa gttttccagg gaaaaatatc cttttcagct tagtacttat tcaaatgatg
     7981 attcccggtt ccatttttat tactccacag tatttattag cacaaaaatt gggtatttta
     8041 aatacaattc cagcattaat attaccaggg gtagttagtg cgtttggtac gtttttatta
     8101 agacaatttt tcttaggttt acctgatgaa ttggaggaag cggcctattt agaaggtgcc
     8161 aatatttgga cgatcttttt caaaattatg ttgccgcttg ctaaatcagg attggttgca
     8221 cttggaatat tcacagcgtt gtttgcattt aaagatttaa tgtggccatt gattgtcaat
     8281 acttcaattg ataagatgcc tttagctccg ggattagcaa acttacaagg acaatatgcc
     8341 acgaacttcc cacaattaat ggcaggttcg ttattagcaa tttggccaat agttgtgata
     8401 ttcatcgttt tccaaagaaa attcattgaa ggaattgcta caacaggtag taaattataa
     8461 aactattaat tagtgattgg agagtaagta catgacaaaa attacattta ttggtgcagg
     8521 aagtactatt tttgcaaaaa acgtattagg cgatgcgatg ttaactccaa gtctacaaga
     8581 tgcacatatt gcattgtacg atattgatga aaaacgacta aaagaatcaa atttaatgct
     8641 taaaaatatt aataaaaatt cgaatgaaaa tcgtgctgat attgtttctt attatgatcg
     8701 caaagaagcg ttaagagagg ctgattttgt tatcaatgct attcaagtgg gtggttataa
     8761 accttcaaca gtgattgatt ttgatattcc taaaaaatat gggttgcgac aaactattgg
     8821 tgacaccatg gggattggtg ggatttttag aaccctacgg acactaccag taatgttaga
     8881 ctttgctaaa gaaatggaag aagtttgtcc aaatgcttgg ttattgaact atacaaatcc
     8941 gatggcgatg ttaaccatgg gtgttttaaa agcgacgagt attaagactg ttggtttatg
     9001 tcatagcgtt caagtatgcg taccagaatt gtttgagcat ttaggaatag ccgatcaata
     9061 tgacttaaat gactttcaat ggaaaattgc aggcattaat catatggcat tcttgttgga
     9121 aattacaaaa aatggggaag atttctatcc aacaattcgt cagttagctg cagaaaaacc
     9181 aaatccacat ccagattctg tacgttttga attgttgaaa cgttttggtt actatattac
     9241 agaatcaagt gaacacaatg cagaatacca tccatacttt attaaatcaa aatacccaga
     9301 attgattgac gaattaggga ttccgttgga tgaatactta cgtcgttgtg aacaacaaat
     9361 taatgattgg gaaagcatgc gagaggacat tgtaaataat ggggagttaa cacatacgag
     9421 aagccgtgaa tatgcttcat atatcatgga ttctattacg acaggaactc cgtcagtgat
     9481 tgcaggaaat gtcttaaata aaggcttaat cacaaactta ccgacagatg tttgtgtgga
     9541 agtaccttgt ttagtcgata aaaatggcgt gcaaccaacc tatgtcggtg aattgccaac
     9601 acaattagct gctttaaatc ggacgaatat caatgttcaa gaactgacag tagaagccgc
     9661 tttaacacta gataaatcaa aaatttatca agcagcgtat ttagatccac atctatcatc
     9721 tgaattgtct ttaacagata tcaaaaattt agtggatgat ttaatcgaag cacatgggga
     9781 ctatttacct gaatataatt aaaaagaagc tgaaagagta aggaattttc aattccttac
     9841 tcttttctta gttaaattta gtaaaaatat tgacaaaaca cttagtaaac agttacatta
     9901 gtgttaacaa aaagaaaacg cattcaaatt gtataaggag gagcataaaa tgaagtggtc
     9961 taaaaaaatt attttagctt gtatagcact aggagcaacg atctctttga ctgcttgtgg
    10021 aaaaaatcag gaagatgcaa gcggtaaggt agaaatagag ttttttaatc aaaaaaaaga
    10081 aatgactcaa acgattcagg agattgctaa agattttgaa gcaaaaaacc ctgatattca
    10141 tgtaaaagtt gtggatgtac cgaatgctgg tgaagtaatt aaaacacgta tgcttgcagg
    10201 cgatgtacca gatgtcatta atctttatcc tcaaagtata gaattgaaag aatgggcgaa
    10261 agctggttat ttagaggact taaccgaaga accgtattta gagaatatca aaaatggata
    10321 tgcacaacgt tttgctattg aggatcgtgt atatagcata ccattaaccg ctaatgtcta
    10381 tggtttttac tataacaaaa ctgcttttga ggaaatgggg attcaagcac cagaaacttg
    10441 ggcagaattt gaaaaaatag tagctgagat taaagatcaa aacaaagtac cgtttgcgat
    10501 tgcaggagca gaaggctgga ctttaaatgg ttatcaccaa ctagctatag cgactgctgc
    10561 tggtggtgaa gaggaagcga acaatgtttg gcgtttctca gaagttaatg gaattaatgc
    10621 tgaaagcaaa gaaatgcaaa aagattttaa tcgattagat ttgttaagag aaccaaaagc
    10681 tttgcaaaac aactggcaag gtgccggtta taacgataca gtagttactt ttacaaaagg
    10741 tgatgcgttg attatgccga atggttcatg ggcaatgccg atgattcatt cacaaaatcc
    10801 agattttgaa gtaggaactt ttccttttcc agcagatgaa gcaggtcaaa gtctaacgat
    10861 tggcgcagga gacctagcct tatcagtttc tgcaacttct caacataaag aagaagccaa
    10921 acgttttgta gaatatatga caacacctga agcgatgcag aaatattatg atgtagatgg
    10981 tgcaccgtgt gctgttgagg gcgttatcga gaatactcag gattcaccat taagtggttt
    11041 aactgaactt gcttttactg atcgtcattt agtttggtta gccaaagatt ggaatagtga
    11101 aaatgatttt tataccttaa caacaaatta tttacacaat ggcaatcaaa aagccatgat
    11161 taacgcatta aatgctttct ttaatccaat gaaagctgac ggaaggtagg aaaataaaat
    11221 gaaaataaaa gaaattttaa atcgttactg ggcaatcatt tttgtagcaa ttccaattat
    11281 tttgcagatg atttttttct actttccaat ggttcaaggt gctttttaca gtttaactaa
    11341 ttggactggt ctgacttata attttgattt tgtgggtttg aacaattata agattctttt
    11401 ttctgatccg aagtttatca aatcgattgg cttcacttta attgtgacgc tttgcatgat
    11461 tgctggacaa attggttttg gtattttgat tgcgcgggcg ttgaacgcaa atattaaagg
    11521 gaaaacattc tttagagcat ggttcttttt tccagcggtc ttatcgggcg tggttgtttc
    11581 tttagtattt aaacaaattt ttaactatgg tttaccaaaa attggtagta tgttaggcat
    11641 tgaattttta caaaccagtc ttttaggaac aacatgggga gcgatttttg cagtaatttt
    11701 tgttttactt tggcaaggaa ttgccatgcc agtcatcatt ttcttagcgg gttttcaaag
    11761 tattccacaa gaaattattg aagctgctag aatggatggg gcgaatgata agcaaatttt
    11821 ttggaatatt gaaatcacct acttattacc aagtatttca atggtattta ttatggcatt
    11881 aaaaggtggc ttaacagcat ttgatcaaat ttttgcttta actggtggtg gaccaagcga
    11941 tgccacaaca tctttggggt tacttgttta taattatgct tttaaaaaca atcagtttgg
    12001 ttatgcgaat gctattgctt tagtcttatt tatcttaatt gggattgttt cactggcaca
    12061 attaaaatta tcgaaaaaat ttgaaatcta ggaggtttcc caatgaaaac atcaaaagaa
    12121 aaaaatattt ctaaatactt gcttttatta ttagggggct tcatgatttt agttccctta
    12181 gtttcaacgc tattcggttc atttaaaaca acacccgata ttatgaataa tttctttcgt
    12241 tttcctaatc cagcgacttt ggaaaatttc cgaagattgt tagcggatgg aattggtcat
    12301 tacttttgga attcggcaat catcacaatc attgcagtag ttgctgtgac agcaattatt
    12361 cctatggctg cttattcact agcaagaaaa atgactcgtg ggaaagcata cggattgatg
    12421 tatttatttt taattttagg aatttttgtt cctttccaag taattatgat tccgattacc
    12481 gttatgatga gtaaaattgg tttagcaaat atgtggggat taatcttact ctatctcact
    12541 tatgccgtac cacagacact atttttatat gttggtttta ttaaactaaa tgtgcctatc
    12601 agtctggatg aggctgctga aattgatggg gctagtcact ttacaactta tttcaaaatt
    12661 gttttcccaa tgttaaaacc aatgcatgcg acgactatta ttcttaatgc tctatggttt
    12721 tggaatgatt tcatgctacc attattaatt ttaaatcgtg attctaatat gtggacacta
    12781 ccattgtttc aatataatta tacgggacaa tattttaacg actatggtcc aagttttgct
    12841 tcctatacag taggaattgt cacgattaca ctagtttatc ttgctttcca aagaagtatt
    12901 atagctggaa tgagcaatgg agcagtgaaa taaccaataa attattctca ttataaagga
    12961 gttcataaac ttatgattat atttgacaat cacacacaaa catttcattt acaaaataaa
    13021 aaaattagct atctattagg aatcgaagaa aataattatt taacgcatct ttattttgga
    13081 aaaaagatta cgcactattc tggtgggtat cgctaccccc gaacagatcg ttccttctca
    13141 cccaacaatg caaatgcgca agatcgttta ttatctttag acacattacc attagaattt
    13201 ccaggatatg gacatggtga ttttagagaa ccggcgcata atattaaatt aggaaacggt
    13261 agtcgtgtaa atgattttcg ttatgatagc tacgaaattc aaaaaggaaa gcccagctta
    13321 gcgggattgc cagccttata tacggaagat gatgatgaag ctgaaacgct gattattcaa
    13381 ttgaaagacg cgattagtgg attgacttta cagttgttct acacaattta tcaaaatcac
    13441 agtgtaatta ttcgttctac gcaattagta aataatagtg atgaaacagt ggagattaac
    13501 cgcttagcta gtcaatcaat tgattttcca aatcgtccac tcgagttaat tcatttaaat
    13561 ggtacctggg caagagaacg tcaaatgact agagaaaaaa ttcatacagg gataaaagta
    13621 ttagatagta aacgcggatc aagcagccat caacaaaatc cgtttgttgc tttagtagat
    13681 gccaaaacag atgaatttca aggagaggca tatggattta atttagttta tagcggtaat
    13741 cacgaaacgg tgattcaaaa agatccattt gaccaaacac gggtcattat gggaattaat
    13801 tcatttaatt ttggttggca actaaaaact ggcgactcat ttcaaacgcc agaagtcgtg
    13861 atggtttata gtaatgaagg tctgaatacg ttatcacaga cgtatcatga attgtataat
    13921 caccgtttaa ttcgtggaaa ctatcgttta aaagaacgtc ccacgttaat caacaactgg
    13981 gaagcaacgt attttgattt taacgaagaa aaaatcatga atattgtcga tgatgcgaag
    14041 aacttgggca ttgaaatgtt tgttttagat gatggttggt ttggcggtcg tgaaaatgac
    14101 ttcacttcat taggtgattg ggttgaaaca gaaggcaaac ttgcttgtgg tttggaaaat
    14161 ttagccaagc aagtgcatga taacgggatg aaatttggga tttggtttga accggaaatg
    14221 atttctgaag atagtgaact atttcgagca catccagatt gggcattggg tattccaggt
    14281 cgtgaccgct caattagtcg aaatcaatat gttcttgatt tttcaagaca agatgttcgt
    14341 gataatatct atcaacaaat gacggcgatt tttgatcgtg taccgattga ttatgtgaag
    14401 tgggatatga atcgaaatat gacagaagtc tattctgtct tgttggactc aacgatgcaa
    14461 ggtgaagtgg ctcatcgtta tatgttaggg ttatatgaat ttttagaaaa gttaacaacg
    14521 aattatccaa ctattttatt tgaaagttgt tcgggtggag gcggtcgttt tgatgcaggt
    14581 ttcttgtatt acatgccaca aacttggacg agcgacaata ccgatgccgt ggctcgtctg
    14641 aaaattcaat atggaaccag cttagtgtat ccaatttcaa gtatgggtgc gcatgtttca
    14701 gcagtcccaa atcatcaaac aggaagagaa acgagtttaa ctattcgtgg caatgctgcg
    14761 atggctggtg tttttggtta tgagctagat ctaacgcaat taacttctga agaaaaagca
    14821 gaaattaaag aacaagttgc cttttataaa aagcatcgcc aacttttgca atatggaact
    14881 ttttatcgct tggaaagtcc gtttgaaggc aatgatactg catggatgtt tgtctcaaaa
    14941 gaccaaacag aagccatggt cttttatttc cgagtattag cagaagctgc gcatccatta
    15001 gtcacattga aattagcagg attagcagaa gaagcaactt atcaaatgga agatgtaaca
    15061 tttgctggcg atgaattgat gaatctcggt ttttatattg actcagaact acatggtgat
    15121 tatgcgacac aacgctttta tttgaaacag atatagtagc ttgaatttac aatataagtg
    15181 caacaccaaa agagactcat tccaaacgaa gaaaatatgt tttatttaca cgcatttact
    15241 aaaaagcagc ctgatttgaa ttgggaaaat gaagaagtac gtgaagaaat ctatgcaatg
    15301 attaactatt ggttagataa aggactaggc ggatttagaa tcgatgcgat attaaacatc
    15361 aaaaaaaaac tagagtatgg catctttgaa tctgatggag aagatgggtt agccttcatt
    15421 gggccctgga ttcttaatca gtcgggcatt gaagtgtggt taaaagaaat gagagagcgg
    15481 acatttaaac cacataatag tatgacggta gcagaagccg atgttcctaa tgaacgttta
    15541 gatgaatata ttggtgaaga tggctattat tcgatggttt ttgattttag ttatacggat
    15601 attgatgtac ctgaaactgg tgaatggttt aaagatagcc aatggacatg gacggatatg
    15661 cgtaccaata tttttacgaa tcaattagtt acgcaagata aaggttgggg cgccttatat
    15721 ttagaaaacc acgatcaacc acgttcaatt aataaatata ttccagaaga atggatcaat
    15781 gattatagta aaaaaatgtt ggcgacgctt tttatgttac taagaggtac cccttttatt
    15841 tatcaaggac aagaactagg aatgaccaac atagaaatgg catcattaga agattttgat
    15901 gatgtagcta cgcattctca gtataaacgg gcattagaat atggattggc accagagcaa
    15961 gcattgaaag ctgtcaataa acgaagtcgg gataattctc gcacacctat gcaatggaat
    16021 acgcaaaaaa atgccagatt ctcaacttct gataatgtat ggttaaaagt aaatcctaat
    16081 tatccagaac tcaatgcaga agctcagtta accaatcaac agtcggtatt caattattat
    16141 catcaattaa ttaaattaag aaaaaatagt gtatacaaag acgtgctaat ctacggacaa
    16201 tttctaccag tgaaagataa aaatgaacat attttgcttt atgaacgcca attagaagat
    16261 aaaatcgtat tagttatctt aaacgttaca gcaacagaac aagaatatta tgtagatgaa
    16321 cagtaccaac aagtattaat caataattat gaagatgttt tattagatca aagtcgaaaa
    16381 ttgcgtttac gaccgtttga aagtattgta ttagctaatt atggaggaca attatgaaaa
    16441 ttaaaaacga agcaatgctc atcacctatg cagatagttt aggaaaaaac atgggcgaat
    16501 taaaacaagt cttagacaaa catttacaag gtgttattgg tggtgtacat ctgttgcctt
    16561 tttttccatc gacaggtgat cgaggctttg cgccgagtga ttatacgaca gttgatccag
    16621 ctcttggaac atgggaagat gtggaagcat taggtgaaga ttattatttg atgtttgatt
    16681 ttatgattaa tcacatttct cgtgaatcaa aatttttcca agatttcaaa aaaaaccatg
    16741 agcagtcacc atacaaagaa atgtttatcc gtattcatga atttttccca gaaaatcgac
    16801 caacacaaga agatattgat ttaatctaca agcgtaaaga caaagcgccg tttcaaacgg
    16861 tagaatttgc ggatggtacg acagaagagg tttggaatac ctttggggaa gaacaaattg
    16921 atttagatgt gaccaaagaa gttgtgaaag aatttattcg agaaaccatc aaagatatgg
    16981 cgaaccatgg ctgttcttta attcgattag atgcgtttgc ttatgcgatt aaaaaattag
    17041 ataccaatga tttcttcgtg gaaccagata tttgggaact tttagacgaa gtgcgtcaag
    17101 aagcagcgaa atatgacgtc gaattattac cagaaatcca tgagcattat tccattcaaa
    17161 tgaaaattgc caatcatgat tattatatct atgacttcgc cttgccaatg gtcactctgt
    17221 attcacttta tagtggaaaa tcagaacgtt tagccaattg gttgaaaatg agtccgatga
    17281 agcagtttac aacattggat acgcatgatg gaattggtgt tgtggacgct cgtgatttat
    17341 taacagatga agaattggat tacacctctg aggaattgta taaagtcggg gcgaatgtga
    17401 agaaggtcta ttcaagtgcc aattacaata atttagatat ttatcaaatt aacagcacct
    17461 attatagtgc attaggcgat aatgaccgta gctatctgtt agcacgtgcg attcaagtat
    17521 ttgcgccagg gattccacaa atttattacg ttgggttatt agcaggaaaa aatgatattg
    17581 aactgttgga agcaacgaaa gaagggcgta atattaatcg tcattactat gatttagccg
    17641 aaattgaaga agaagtgcaa cggccagtga ttcaacaatt atttgcactt ttaaaattta
    17701 gaaatgaatc tgcggcgttt gatttggatg gaacgattga agtagcaacg ccatcagaat
    17761 cagaaatagt gattacacgt aaaaataaaa acggtagtga tagtgcaaca ttaacggcta
    17821 acttggatac gaaagaattt atcattgagt ctaaagatat aaaaattttc actagtgttg
    17881 cattaaaatt ttaacctatt ccatagaaca aaaaatcctt tatactagca cttggatgac
    17941 ttatccaaga ccaataaaaa ggactcatac atggataagt ataaaacaaa tacagcattt
    18001 aataaatggt tttctgccat aaaattagga aaactttctg tcaactccca aaaatgtatt
    18061 tttgattaca attcgtatcg taaaaaactg tcttttgaag ctgtgctcaa gctctttctc
    18121 tacgcagtta atgaagaaaa agaaagtttg cgtgatttga gtacagctct tatcgaccga
    18181 tcgctccaac tagaaaccga tgtgtcatcg attagtcata cccaattgtc tcgagcattt
    18241 aatgcacttg aacctaaagt gttagaagaa atttttcaac aactgttaga aaaagttcaa
    18301 catcaaatca aaccaaccaa gcgaaatcat ctgtatctga ttgattccag tacgttttcg
    18361 cttagtttaa aacgtcataa gtgggcgact ttccgtcaaa caaaatcagg tgtcaagtta
    18421 cacttaaact attgttatat ggacgatact acgatgtatc caactgattt taccttaaca
    18481 aatgcccatg aacatgatct caatcaacta ccagtattgg tgaatcaacc agaggcaact
    18541 tacgtgtttg atcgtgggta ttaggacttt gaaaagatgg atcagatgca ttgggatggc
    18601 tatttcttcg tcactcggat taaaaaaaac accaaagttc acgtgataga tactctggaa
    18661 acatcgccag aaacagaaat tctacgagac gagctggttc gtttaggatc taaaacctat
    18721 ctaaccgcca atttccgact agtcactgtt caagataaga acggacgtgt gtttcaattt
    18781 atcaccaatc gaatggacgt ctcttcaaaa gaaatctctg atatgtatca cgctcgttgg
    18841 caaatcgaat tgtttttcaa gcacatcaaa caacacatga ccattaaaac cttcttttct
    18901 caaagtgaaa aaggcgtgca gaatcaattg attttaacga tgatttccgc tctcctcacg
    18961 tttctcatca aattggagac gaaaacggaa aaatcagtgt tccaaatcaa acgattcttt
    19021 cgttatttac tgtttcagcc ctttgaatgt tgtctagaaa aattaattcc cacatgagaa
    19081 aaaagaaatt tggctcgtta cttttggttt tcagttgaat aacagataaa aaattttctg
    19141 gaaaaagtca tcctctgcgt cagcacagtt ttttttgact tattttcaga ggaaatagtt
    19201 ttcagaatat tctaaaatgt tttatgcaac actagtgata ttttttagaa ttagatttct
    19261 aatgtgcatt ttttgagtat taaggagcaa gtatacaaag atttataaag ctgattaaat
    19321 ttgaataaaa atatgcagga acgctactat ttttagtagt gttctttttt atatttgaat
    19381 aaaaaatgtg gtggttaaat ggtggttata acaaaaaatg aattaatgaa attaggttat
    19441 gggaagtatc agtctgagag aatcatacga caggcaaaag caattatggt ttcaaaaagc
    19501 tatacctatt acgaaaatag aagattagga ctagtacctg ttgaatctgt agaagaagtt
    19561 ttaggtatga agatagaggt agaggaggat tttcagaatc atggttaatt ttaaaaaaga
    19621 taaaaatggg aaatatcatt ttactgctag tttgggattc gacaaacgaa cagggaaaag
    19681 agtacaaaaa aacgtagagg ggttaaaaca gttaaagaag aaaaaaagcg tataccgaac
    19741 ttgtttctaa ttatggaaaa gaagcagaaa cttctcaaag tactatgttg tttgaggagt
    19801 ttttttataa attattccta ccttattatc aagggaaagt agaagaaaga acatataaaa
    19861 atcggttatc tacaatcaaa aaaacttttc ttatttcttt aaaatggagt taagagaaat
    19921 acgtccaatt gacattcaaa agtggcaaaa taatttattg agcaaatata aaaattctta
    19981 tgtaagaaac atgtatggga tttttcagat gtcattagat cgagcagttg ttttgggact
    20041 acttgaaaaa aatcctgcaa aagttattgg aaatgtgaag aaaaggaaaa ctacagttga
    20101 tttttggaca aaagaagaat ttacaaaagt tatttcaact ttttatattg atgatttgta
    20161 ccaacatttt ggttttattt gtatgtggtt actttttatg accgttatgc gtttgggaga
    20221 agccactgca ttaacttgga aagatattga ttttgaaaat aaaaacttgc gtgtgaataa
    20281 aacactctat tataagagag ccaaaaccta tgagtttgta gaacccgaaa cggcttctag
    20341 tattcgtaca atcgaactag acgacaatac aattaccttt ttaaaagctt ggaaggagcg
    20401 tcaagtttca ataggaggca tgtcgtttgt actttcttat aatggaacac ctactcagaa
    20461 atatacaatc cgtcatatta tgagaagaca tgcaacgatc gttggtgttc atataatccg
    20521 catacacgga ttaaggcata gtcatgcttc tttgcttata tcacaataaa aagatgcact
    20581 tcgaattaaa gaaaggttaa aacatgctga tgtacaaacg actttaggaa cttatggtca
    20641 tttgtatcta aattcattga gagagataac aaatgatccc acagattata tccaaactaa
    20701 tttttctaaa atcaaaccga cacctattta cgataataaa aaataaatga taaacaaaag
    20761 taggatcaca ccaaaagaaa cacactagaa aaactataat acaggctttt ctagcgtgtt
    20821 gtttcttatt tccgcctatt tatacattat actcataaag catttaatgc atcgatcgta
    20881 gccgcattgt ctatatgggt atataaagtt gttgtattgg tacttgaatg tcccaattgt
    20941 tgggcagtta ataccaatga atttgtctgt tggtagagtc tagtagctaa tgtatgacgc
    21001 agtttgtgag ggctgacttg aattctaaag ccttttgaat attttgctac catccgttcg
    21061 attgcaatac cggatatacg cttggcttct ccttggtaaa ttgctagaaa taaagctttt
    21121 tcgttttctg ttactttata tcgttctttc cgaatagcta gataattagc taaatattcc
    21181 attgcgaatg ctgctatatt gacagcatct cttttatttc cttttctagt tactaccaca
    21241 ttattcttat ctaaagatag gtcatctaaa ttaatattta taacctcgga gagtctcaat
    21301 ccagatccta aaatcagtgc attgatcgcc aggtctcttt ctacatctcg ttgataatag
    21361 atcaaagctc gtttactcaa agtattttta tattcattgg caatataatc tagataattt
    21421 tgtgtatcct tatctagaaa taattttttc tcaatggcat tggcacgtgc agctaacgta
    21481 gttttatctg tgatggttgc tacttgatcc ataggattat tgtctaagta tggttttcct
    21541 agaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc cgtgatattt
    21601 ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt
    21661 gtctcttttt ttacattttc ttttgtgcga cgtttcaaat gagatttgta caagtttagg
    21721 tcataggcag ttaactgtct tatcgaggtg atagaagtat caggaacgat agactcactc
    21781 agaagccatt cataaaagag cttataattt tttagatatt ctaaagtggt tcgaggtgat
    21841 tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg
    21901 attgtatcta ttttttgata tatttctgta tttttcatgt cattcatcct ttcaataagt
    21961 aggggagaag agtatctcta taattggact ataccaatta tagaatgttt tgtcaattgg
    22021 tatgctctaa aatttattaa tttgtttcaa aaaaagcata taaacaggta tacgtttaat
    22081 aatttaaaat aaatggtata cataaatagg gaaggaaata gaagggagca atctaaattt
    22141 tttgaatttt cttaattaat tatatagaaa gaacaatctt tctatagagt tagacatgag
    22201 agggactatg cttgatttaa aatcaacaag cgtatatact gtatatacaa agaaaaagga
    22261 gggagaagac tatggcaatt aaagaaagaa aatttaggga agtggggaac tcagtagtat
    22321 taactatttc aaaggagttt cttaaagaaa atgggttaaa tcccggagat acggtttttt
    22381 tagatgaaga aaaacttcga gcggcaatta caaaaaaaca agccttatct gatgaaattg
    22441 atttagcaat atctcaagca ttaactgaat atgatactac ttttagagag cttgtcgata
    22501 aatgagtatt cattacctag aagccaaaga tattataaaa atgaatgtaa tccaaataaa
    22561 aaaatactct cctaatgaac cccttggtgt aaaggatgcg aatgctttag aaatgtgtgt
    22621 agggcaactt cgagcctcag cttttggtga agaggtttat ccttcgattt atgaaaaagc
    22681 agctatccta cttatacaat tgataaagaa acatcctttt cacaatgcaa ataaacgtac
    22741 tgctttctta gctacttttg tctttttgaa aatcaatgga cagcttttag ctattgatca
    22801 aaaagatgct gtaaatttag cggtatatat tgctacgtat gataaggatt tcgatcaact
    22861 aaaatatgaa gtgatcaaca ctataaagac acatacgcag cccatttaag gagttactgt
    22921 atgtgtcttt atagggttat tagctgctat agcgaaataa ttgaagtaaa ttacaaatag
    22981 ctataattca aatccaacta tatagaaaac gtaaattttc tatatagttg gattaagata
    23041 acgactattt attttatcat atttttacta taccctatac ttttacttgg tatatttatg
    23101 aataatcgca aatttacccc tatactacta gtttgtaagt tagattaata aaaatactaa
    23161 agttatttgt tcttttatta aatacaccta caacttataa attgatggta tttagataac
    23221 acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta ctcaatcgaa
    23281 agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga ccttttacat
    23341 cttgcagaaa tcgttactgg gattaaacta agcaataaaa actaatgttc ccctcattct
    23401 ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc ttcaggatct
    23461 tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg ataggcgata
    23521 ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt
    23581 tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat cgttcttaac
    23641 aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg ccttaccctt
    23701 catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg gcggtctgaa
    23761 aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc tttgtagcgg
    23821 atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa
    23881 aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca cttgcattca
    23941 cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt actgttaaca
    24001 tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca aagctaatct
    24061 cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat ttacatgaat
    24121 aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg
    24181 tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat catcaatatt
    24241 acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat ttgtaaacca
    24301 cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt taacagataa
    24361 tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa cttgatatgc
    24421 taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca attcttcagg
    24481 aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt tttttaattc
    24541 ttctttattt ttatatgttc tcattatatt acctccataa attcctaatt gtctccttta
    24601 attcatctat aatttcatcc acgatatttt aatccgttct acaatgcata tctgatatgc
    24661 attgactgtc taactcatat gtgaatgatt tataaaatat cccaacgtct catctaaaga
    24721 aatgttgatc gtcagcaagg agctaaaaaa tacgaatata tttcttttgt agtaaaatcg
    24781 gactggtttg catatcattt tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta
    24841 ctaataaaaa ctaccacttt aagcgttaag aactgttaag tttagttaag tgaggatagt
    24901 tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga
    24961 ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt
    25021 gacgatgaca cgttccttac ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa
    25081 ctcgaagaaa atttaattct aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact
    25141 attaaaggtc aagatttacc tgaagatcat cctttttagg aaattgaagc tttttatgtt
    25201 gcacaatgca tttataatac tacaatgatg ttttcaccag atattcttct agggtgcggt
    25261 gtaatgaaac agatacactt gcaaaagaaa attcaccgcg aatttactaa aattatgaat
    25321 aattatgtta atcttccgaa atttttttcc aaaattaagg gatcaagctg gtgttataga
    25381 ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa ccgatactct
    25441 gttaagttgg ttgacacggt gtcgattttt atgactttct tattctgaaa cttcactaac
    25501 ggacagtacc taaaaagtct atagtatgga cgtttacgag tactatttta tcaaaaatgc
    25561 tgctcatttc aacttatatg ttttagtgaa ctacccccac ttagcttgga taggctacaa
    25621 ttaactagac agaaaaatta aggtgtgtag actagaagaa aacataccag gaggaatttt
    25681 tatgtctaag agaacacgaa gaactttttc acaagaattc aagcaacaaa tcgtcaatct
    25741 ttacttagct ggaaagccac gtgtagaaat cattcgagaa tatgaactaa cggcttcagc
    25801 atttgacaaa tgggtaaagc aatctaaaac gagt
//
LOCUS       DACCVU010000032        25319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCVU010000032 DACCVU010000000
VERSION     DACCVU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.032"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            237..1877
                     /locus_tag="IVW29_001156"
     CDS             237..1877
                     /locus_tag="IVW29_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7838649.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVW29_001157"
     CDS             2031..3353
                     /locus_tag="IVW29_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7838650.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVW29_001158"
     CDS             complement(3415..3846)
                     /locus_tag="IVW29_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838651.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVW29_001159"
     CDS             complement(3910..4746)
                     /locus_tag="IVW29_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7838652.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IVW29_001160"
     CDS             complement(4936..5238)
                     /locus_tag="IVW29_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7838653.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVW29_001161"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVW29_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7838654.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IVW29_001162"
     CDS             complement(5692..5958)
                     /locus_tag="IVW29_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7838655.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IVW29_001163"
     CDS             complement(6212..6937)
                     /locus_tag="IVW29_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7838656.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IVW29_001164"
     tRNA            complement(7303..7373)
                     /locus_tag="IVW29_001164"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IVW29_001165"
     CDS             7490..8242
                     /locus_tag="IVW29_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7838657.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IVW29_001166"
     CDS             8258..8737
                     /locus_tag="IVW29_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7838658.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IVW29_001167"
     CDS             complement(8988..10568)
                     /locus_tag="IVW29_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7838659.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IVW29_001168"
     CDS             complement(10561..11304)
                     /locus_tag="IVW29_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838660.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IVW29_001169"
     CDS             complement(11480..12256)
                     /locus_tag="IVW29_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838661.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IVW29_001170"
     CDS             complement(12504..13340)
                     /locus_tag="IVW29_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7838662.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IVW29_001171"
     CDS             complement(13351..14280)
                     /locus_tag="IVW29_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7838663.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IVW29_001172"
     CDS             14472..14825
                     /locus_tag="IVW29_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7838664.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IVW29_001173"
     CDS             complement(14899..15555)
                     /locus_tag="IVW29_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7838665.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IVW29_001174"
     CDS             15781..17715
                     /locus_tag="IVW29_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7838666.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IVW29_001175"
     CDS             complement(17801..19024)
                     /locus_tag="IVW29_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7838667.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IVW29_001176"
     CDS             complement(19018..20916)
                     /locus_tag="IVW29_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7838668.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IVW29_001177"
     CDS             complement(20910..22028)
                     /locus_tag="IVW29_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7838669.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IVW29_001178"
     CDS             complement(22111..22944)
                     /locus_tag="IVW29_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838670.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IVW29_001179"
     CDS             complement(22937..23854)
                     /locus_tag="IVW29_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838671.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="IVW29_001180"
     CDS             complement(24081..25205)
                     /locus_tag="IVW29_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7838672.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7808 a         5047 c         4308 g         8156 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggc
//
LOCUS       DACCVU010000033        25223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCVU010000033 DACCVU010000000
VERSION     DACCVU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.033"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(191..1210)
                     /locus_tag="IVW29_001181"
     CDS             complement(191..1210)
                     /locus_tag="IVW29_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7838673.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1207..1431)
                     /locus_tag="IVW29_001182"
     CDS             complement(1207..1431)
                     /locus_tag="IVW29_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7838674.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1428..1721)
                     /locus_tag="IVW29_001183"
     CDS             complement(1428..1721)
                     /locus_tag="IVW29_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838675.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1758..1895)
                     /locus_tag="IVW29_001184"
     CDS             complement(1758..1895)
                     /locus_tag="IVW29_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7838676.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1897..2328)
                     /locus_tag="IVW29_001185"
     CDS             complement(1897..2328)
                     /locus_tag="IVW29_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838677.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2342..2836)
                     /locus_tag="IVW29_001186"
     CDS             complement(2342..2836)
                     /locus_tag="IVW29_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838678.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2836..3285)
                     /locus_tag="IVW29_001187"
     CDS             complement(2836..3285)
                     /locus_tag="IVW29_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838679.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3289..3906)
                     /locus_tag="IVW29_001188"
     CDS             complement(3289..3906)
                     /locus_tag="IVW29_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838680.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3906..4814)
                     /locus_tag="IVW29_001189"
     CDS             complement(3906..4814)
                     /locus_tag="IVW29_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7838681.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4827..7586)
                     /locus_tag="IVW29_001190"
     CDS             complement(4827..7586)
                     /locus_tag="IVW29_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7838682.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(7583..8287)
                     /locus_tag="IVW29_001191"
     CDS             complement(7583..8287)
                     /locus_tag="IVW29_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7838683.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8299..10608)
                     /locus_tag="IVW29_001192"
     CDS             complement(8299..10608)
                     /locus_tag="IVW29_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7838684.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(10848..11267)
                     /locus_tag="IVW29_001193"
     CDS             complement(10848..11267)
                     /locus_tag="IVW29_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838685.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQ"
     gene            complement(11267..11893)
                     /locus_tag="IVW29_001194"
     CDS             complement(11267..11893)
                     /locus_tag="IVW29_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7838686.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(11900..12265)
                     /locus_tag="IVW29_001195"
     CDS             complement(11900..12265)
                     /locus_tag="IVW29_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7838687.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(12255..12593)
                     /locus_tag="IVW29_001196"
     CDS             complement(12255..12593)
                     /locus_tag="IVW29_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838688.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(12583..12909)
                     /locus_tag="IVW29_001197"
     CDS             complement(12583..12909)
                     /locus_tag="IVW29_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7838689.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(12887..13168)
                     /locus_tag="IVW29_001198"
     CDS             complement(12887..13168)
                     /locus_tag="IVW29_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7838690.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(13170..14534)
                     /locus_tag="IVW29_001199"
     CDS             complement(13170..14534)
                     /locus_tag="IVW29_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7838691.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(14547..15122)
                     /locus_tag="IVW29_001200"
     CDS             complement(14547..15122)
                     /locus_tag="IVW29_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP7838692.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(15088..16275)
                     /locus_tag="IVW29_001201"
     CDS             complement(15088..16275)
                     /locus_tag="IVW29_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7838693.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(16339..18066)
                     /locus_tag="IVW29_001202"
     CDS             complement(16339..18066)
                     /locus_tag="IVW29_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7838694.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18063..18515)
                     /locus_tag="IVW29_001203"
     CDS             complement(18063..18515)
                     /locus_tag="IVW29_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP7838695.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(18627..19007)
                     /locus_tag="IVW29_001204"
     CDS             complement(18627..19007)
                     /locus_tag="IVW29_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7838696.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19004..19390)
                     /locus_tag="IVW29_001205"
     CDS             complement(19004..19390)
                     /locus_tag="IVW29_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838697.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(19392..19670)
                     /locus_tag="IVW29_001206"
     CDS             complement(19392..19670)
                     /locus_tag="IVW29_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838698.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            19718..20446
                     /locus_tag="IVW29_001207"
     CDS             19718..20446
                     /locus_tag="IVW29_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP7838699.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(20592..21068)
                     /locus_tag="IVW29_001208"
     CDS             complement(20592..21068)
                     /locus_tag="IVW29_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP7838700.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(21028..21375)
                     /locus_tag="IVW29_001209"
     CDS             complement(21028..21375)
                     /locus_tag="IVW29_001209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838701.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(21362..21547)
                     /locus_tag="IVW29_001210"
     CDS             complement(21362..21547)
                     /locus_tag="IVW29_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838702.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(21548..21820)
                     /locus_tag="IVW29_001211"
     CDS             complement(21548..21820)
                     /locus_tag="IVW29_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP7838703.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(21817..22020)
                     /locus_tag="IVW29_001212"
     CDS             complement(21817..22020)
                     /locus_tag="IVW29_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838704.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            22145..22357
                     /locus_tag="IVW29_001213"
     CDS             22145..22357
                     /locus_tag="IVW29_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838705.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(22390..22584)
                     /locus_tag="IVW29_001214"
     CDS             complement(22390..22584)
                     /locus_tag="IVW29_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838706.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(22592..22780)
                     /locus_tag="IVW29_001215"
     CDS             complement(22592..22780)
                     /locus_tag="IVW29_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838707.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(23407..23772)
                     /locus_tag="IVW29_001216"
     CDS             complement(23407..23772)
                     /locus_tag="IVW29_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838708.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(23812..24114)
                     /locus_tag="IVW29_001217"
     CDS             complement(23812..24114)
                     /locus_tag="IVW29_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838709.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(24114..24413)
                     /locus_tag="IVW29_001218"
     CDS             complement(24114..24413)
                     /locus_tag="IVW29_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838710.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(24576..24845)
                     /locus_tag="IVW29_001219"
     CDS             complement(24576..24845)
                     /locus_tag="IVW29_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838711.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(24857..>25223)
                     /locus_tag="IVW29_001220"
     CDS             complement(24857..>25223)
                     /locus_tag="IVW29_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838712.1"
                     /translation="RELPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDYQE
                     RIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQE
                     AYLKKKALERANRQSKAPF"
BASE COUNT         7028 a         5382 c         3904 g         8909 t
ORIGIN      
        1 actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg gcaacaaacc
       61 tgttatcgca tagcttggag tgtgactatt atgggtgata gtgaagatat gcgataacat
      121 cactatttta tcgaatgatt tttatagttg tcaatatagt tatgtaatgc tcctcaacga
      181 ggagctattt ttatcgttta ggaatattta aataccaacg tttgtcatgg aaatcttgcg
      241 caccgccttt agtgtttccc tctggatcat tcgttgcccg catcatgaca tagactttct
      301 tattagggaa gttacgcatg ttaaaagata catgatagcc aacatttcca gaagtattat
      361 aagcttgatt tacatctgat ctataaattc catcagctct tactcgagct aattctttcc
      421 cagtattgta atccataatg aagatatact cgtatttata gttagcaatg tgccatccag
      481 ctacatgcaa atttgcattt tcgatttctc caaactgatc aatgtgggcg taattcgttc
      541 catctgtcag tgtaggatta gctgcaccag ctcgtgttgg atcaatgaca ggcttgtttt
      601 cagaagttgt tggattttca tcggtaaatc catgagctaa atcatatgct aatttttctt
      661 tacttacgcc catttcagaa agataaccgt aaggatctgt atgatcgccc cagatgtttt
      721 gtgttaccca taaatgcgat ttgattcccg gttggttata aggagtgtcc aacgttaatg
      781 gaataccata tttcattgct gaatctctag ccaattcaac gtatgccttg tagtttttct
      841 caaacgttgc tttatcatgt gtgtgttgta actcaatctg cacaggactg ttggcattag
      901 catacgaacc agcaccgtac tgtacataac caggttgacc gacttgataa acaattccgc
      961 cgtctcccac aatataagca gtataagcgc tagtccatga acgttgcata tactgcgctt
     1021 cattgcgtcc tgttgctgtt tcattagccg tttcatgcag taaaatatac tgattatttg
     1081 ctacttgtga gctaccttca tttgcgccca aattaaattc attgttgata gtataggcaa
     1141 atccgttaat tggcaataaa aaaagagccg ttaacaggct catcgcagta atagtaattt
     1201 tctttttcat ttgtttcctc cttcttcgct ttcagccgag aacattttgt aggttcgatt
     1261 tgatacaccc aacacactcc ctaaaaacgc gccaaaacca gtaatgatga caacacagat
     1321 atctgtgtac tgccaattga gcgctttacc aactaacccc acgaaagtag ctagtgcggg
     1381 aataattacc agtgcgaacc attttagtac ttcgaacgtt ttattattca ttttcttctc
     1441 tccctaaaca aagttttgat ttgttgcgtg tgttccacca atttttctgt atgtgtatct
     1501 aatctttcat cgtgtttctt tagttcttca tgaatcatca atcgatctga tttgctcgat
     1561 tctaaatctt tagtcagcaa atctaaattg tggcttactt ttgaaagagt ctcagtaatt
     1621 ttcgagaaag atgcagcaat cggtctaatt actaataaaa tcaaagaaac gatagcggtt
     1681 attgatcctg ctatcattcc ccattcccct aaattaatca tgtgacaact cctttacctt
     1741 aaataaaaac gcatcaatta agatgcgctc ttatctttat taatgatttt atctgcttct
     1801 tcgtctgtaa tgcataatgg aacgaaaacc attacttgtt cgttagtgaa acagccccag
     1861 tcatacatta gtttgatatc ttcatatgaa tacatgttac ttcccacctt gtaattgttt
     1921 tttgatttct tcgatttcct tggtattttg tacagaagca agcatcgttt ttgaattcaa
     1981 ctgcgctaaa ctttctgact tttctttcaa taagacattt tcttgcttga ttgctacatt
     2041 gtttagcatt gctttagaat ttagctgttt caattcatca ttttctgcct tcagcgtttc
     2101 gtataatgcc ttaatattag ccaattcatc tacgctatca acagcattcc cttttttctc
     2161 ttcttcagta gctaactcaa cccactgttt tttatcaaaa tcaaatcttg gtctccagtt
     2221 aggaactggt ggtttaatct ctgtacagtt ttcagggata ttttcttgat tgttcaaaat
     2281 gatttgctcg aatccgtaag gcttgattga tttgtaaact actttcatta gatttcctcc
     2341 cttaaattga ataagtgata acaaatgaat aagctgatcc ataacttgaa tttcttctcc
     2401 atttaatggc tccgtctgcg ccaatagata gttgagcact gttcaaagta gaacggtcta
     2461 ttgacccaac cagttgttca aaactaattg gtgtccgata gccttctgga acagttaaaa
     2521 taaccgaatc attcccgcca ctactttttc cgtttaaagc cacaaaatat atagaaactg
     2581 tttttccttc acgataaagc tttgctgtcc ctgtattacc gttagtgaca accagattta
     2641 ccacttcata tctgttatca tcagtcgtca aaacacgttt attattgatt gttacagaac
     2701 ctgaaaaatt cttatgtcct gcaatggctt ggtctccatc tgtctgcacc aatgcttctt
     2761 taccatcaat agcttctaca tgcgttttta agtatttagc aactccgtct tcttttaatt
     2821 gaacaatatc agccattaaa ccgtccccac tttctcaaat gtaatattcg ctaatccatc
     2881 aagtttcact ttatcggttg ctgacattaa acctgctgtc gtagttgtag cattacctgg
     2941 atttttctgt gctccagctg caattccatc caacttagtt ttatctgtgg acgacatcaa
     3001 cccatttgct gtagttgtag ctacagctgt agttgtggca tttattccag gatcaccttt
     3061 atctcccttt ggtaaaacaa aattaaatct agctgcagat gatgttccta cattcgtaac
     3121 agaagcggtt gaaccactag aaacggttcc tatggtaatt gttgctgctt ggccaggatc
     3181 gcctttatct cccttcaccg ttgttggttt gccttctata gcattccaat gagtttgtgg
     3241 ataaacctgt acaccgcttt gttttatttt tacgatatct gtcattttct atacctcccc
     3301 gattttttca aaagtaaaat ttggaattct ttcgtttgtg taattttctg cttgatttac
     3361 agcttcttgg aatttttgat ctacatatga ctgattaaca ccaccagtcc cactaccacc
     3421 tgtagaacta attgttccat cttctgcaat tgatatattt gcacctgctt ttaatatttt
     3481 tagagattct aattttccct ttaattcttc agagaaattg aagtctgttt gcttagttgc
     3541 agataaaaca ccttcttcag taacttctaa gagttcccca acttttatac ctcctaattg
     3601 ttcaggagta gcaatcggca aaatatatgt tccaccttct ccatttacga tcttttgaaa
     3661 catttcagca gtaagaatac catcgcttgt ctcgcttgca taaggtagtt cagtaattgc
     3721 attctctaat cctaaatcag ctttcgttaa aatgactgca ccatatttac cattaaccga
     3781 aagaactttt gattgtcctg atatcatttt ttctaagcct aaaactgcag aaacatgtgt
     3841 aattggcata aattgacgtt taacaccaga ttcatcagtc tccatcattc tctttacttt
     3901 aaccatcaaa tcaccccaac tttctcaaga gtgaaaacat tctgttttgg atcatcaaca
     3961 gttgcaataa tcaaagctcc ttcttcaatt ggaaattcta ctgtttcaat tttttcgact
     4021 tcgtgattat ctgaaaagag atcgtcttga agaatatcag tcacttcaat ttcaccgtat
     4081 tcaatcgtga aaagtgctgc tttcattttt tgatacaaat aatttagatc ggataggaaa
     4141 cgttcagaaa tcgattcatg gcgtacacct tgaagatcta cacgtgcatc cattaattcg
     4201 gctaacatta ctccacctgg atcgatagac ttcaaaatat ccttgattga ttcaaaccat
     4261 gacaaataat ctgattcttg tccttctctc caatcttgga aactattctc ctgttcttct
     4321 ctccagcgat caaattcttc tttcctttca ttcatccagt ccgtaaaatc gcctttattt
     4381 tcattaataa aatctgtcat atcagcaatt aaatcttcaa ttgattgcca ataagaaccc
     4441 atttctcctt ctgtttttga aacagcattg atgacaaaat aggagaagtc ttgagttgtt
     4501 ccaatcaagt cttctccttt aaatataatg aaattggctg tttgtcgatg caaacactgc
     4561 atggaatatt tatcaaaaat atatttgatt ttcccttttt tagcatccac aattcttgtt
     4621 tctaattgga ctggatattt tccgccaaca actgattcaa aatatacctt acattctgat
     4681 aaatcatatg gaagaccatt ttcgacaatt gtagcttcca taacttcagt gtttttattg
     4741 ccttgtcgaa cttgaatcat ccccacgtaa ttatagggtt ctgttgtact taatatgaca
     4801 ttccactttg ccattaaatc acctccctat tttggtggta tgacaatgga agaaattgca
     4861 cttgcactat aatactggcg atctaacttt ccacaaatca tacctaactc tgtgttttgt
     4921 tcataagttt gcatacgacc attagctaag cctcggataa cgccagtatg tccataagtt
     4981 ccgtctgcaa accatgaacc cacttgtcca cctcttgccc aattaataat tgcaccaact
     5041 actaattgat catatctagg gttttgaatc actttccaac caacagcaga ccaatcatag
     5101 gcaataccaa tatcagatgc tgcagaagta tttcctatca catgagttaa accatatttt
     5161 gttccagcac ccatgccaca accacccaga tatcctgaat attctgcaga caagccataa
     5221 cactgaccat tgccaattcg ttgtccaatc aaagactcta aatgttttag tcctgcttct
     5281 ccagtagctc ctcctggttt taaatcttta aatttgttat accagttaac tgcataatct
     5341 tgacgttcag gatgtgtcgc tgccggacgt tcatagtttc gttcaaaagc atatgctgct
     5401 tgtcttggat cagtacaggc cttaaatcca tcaaccgtcg taggttgtac tacgcccatc
     5461 cattgtccat ttgtaaatgt ccaaataagc aatcgaactt gtgcatctaa gctcgttatt
     5521 ggttctttaa tacctgcagc attaaataag ttttgaacat aaacttttcc atcccatgtt
     5581 gctggaccaa caagaggata tgaagaacca tcccactgaa ctaatccgta tgctggtcca
     5641 cctatttgaa cagtatcagg atcaaaagtt cctcctgttt cttgttgaat gttccccaat
     5701 attccacatg cagattgttt cgtaaatccg ttattacaca aaatatcgta tattctccaa
     5761 gctctctttt ctgcatctgt ttttaattca tcaggatacc caccaggtgt ttctcctccg
     5821 ccagatggac cgccattttg tcctggtatc acttctttac ctctaacagt aagttttccc
     5881 tgaatatcta aatcaccgaa ataatatgct tttccgtttc ctaataacac aaatcctttt
     5941 tctgcagttg gagaaattaa aatgtatttt ccatcgccat ttgtacgtat taccaaagaa
     6001 ttttcctcaa gaggtgtagg tgttacagca tttggaaatg gatttcctgc tgaatctgtt
     6061 gttccgatcg ttccaatgga ttcttttcta ttccagaatt ccataccttt tttggttagc
     6121 tccatgattt ttttgttttc attccaaatt tgaagcgtgc cttttacaag ttttaacaca
     6181 tcaccataag cattaaagga agtttcgaat acttctgcat ttattgttcc tactttgatg
     6241 aagtcagcaa caatttctcc ttttgatgtg atagcaatac cgaacgggcc atttactccg
     6301 ttgtctgaat aacctaagcc atttaaattc caacgccaca ctcgctttgc attcgccaca
     6361 tttggagtat ccatgataag aatctctgat ggagcttttt ctggacgaaa aacgacatgc
     6421 ccaccagaat taccagtgat ccatgccgtc gcattcaaca cattttgaac taacgtttct
     6481 gttcgattgt cgattttctt tttcaattct tgtgcttgag tatttactgc cgaagtgtac
     6541 agtgataaat cattacctaa aacaatatcc ttatatttcc caagcgttgg gaaataagtg
     6601 tattcgacca tacgctcttt tatctcgata tctagctctt tcgccctaac atgagcgaca
     6661 tcaccaaagt gtaaagacgc tagctcttga tacatgtcac cgtattcgag tgtgtgttcc
     6721 aacgccacca tactgactgt atgagtagct tttggctcat ggatacgatc attgtcaaat
     6781 aaggttttgc cccatttgat caattcatca actgttttac aatctccgtt ttcacgtttg
     6841 gcaatacgtc gattctcgtt ggttactcca tcaatttcta aataaccata ttcgataggt
     6901 tctttgtctt catcataatc gttatcagga acaccgccaa ccaaatacaa actattgaca
     6961 attgattctt catcaatttc ttcttcgatt gcttctaaat tgatgccaaa atcaatccga
     7021 aaaccattat ctgatccaat ctgttttacc agtttcaaat catagttatc catatcaagt
     7081 tccgcgctag tcacgccagt caaattttga ttaccgttat ttgaaccaat gattgcttca
     7141 actggcgcga cttgtcttgc tgtaaattgg tgcgttgtcc ctacattcga aagatagtta
     7201 aatttttgat caaatgccaa actgttttta agattggtca tgatctgaga cccattgccg
     7261 ttatccgtaa acgacttgac aataaagttc ttattggcca taaaaccaat atgccgagct
     7321 gttacagaaa cggaggtcaa attctttttg acattataaa tttcaaaata ttgccatgat
     7381 ccatctggta ccttcgcttt tataaaattt ccttttttta aataagagcg atactgtccg
     7441 cttcttgaat agttaccata aaaacgatat tggccattca gcacacgatt gatttctggc
     7501 aaatcttccc aatcaactaa cgacgctccg ttcacgctca aatcatctgg cattttttta
     7561 taagcataaa taaattcttg cgtcacagat acacactcct attccaaaac cgcacttctt
     7621 taaattttcc ggatattttt acctgattcc attcaggctg caatactggc caatcaccac
     7681 gcgtaaataa attcaaacct tcttgtattg cttttccaag ctgagtatca accacgattg
     7741 ttgcagctaa cgtattaagt attgttaggc ttttatcacc gacagcgatc gttatatctc
     7801 ctccgttcga ttctatttcc agatagggat gtgcaatttc gtcaccatga tcaaaaatat
     7861 ccataacact agaatgaaaa attttaggtg cttcaccgat ttttcttttg agtggctgac
     7921 aacgaaaagt aacatcgaat gtataaaaga agccccactc gttttcgaaa gggacttctt
     7981 tatccatgct gcagatagcc tctagatact tgtctggatc attatgtgtg attaattggc
     8041 ttttaccagt gagccatcgt ttgacttctc tcagtttcga atgtggaata gtgatccctt
     8101 ctatttccaa atcaaaaggt tcatagtcat caaacgtctc cgtcaattct ccgcttcggc
     8161 cctgaatcgt gtaagtttcg tatcgcttgt tcggcataat atctggaagc tctgcctcaa
     8221 tgatgcaatt catatcaatt actgcattcc gatttttcca aataaaattt ggttcatctg
     8281 gattcataaa ttgtcgactc aaataggaac acctcccaaa tcacgaatag cctgtttgtt
     8341 tgctttagca aatttacgat tcatacgatc taactcagat ggattgtttg catcgacttt
     8401 gccaatgtgt atatgctgtt caatgctacc tccagaaact cttccactga tccctttgcg
     8461 tttttcttcg tctgataatg gagtaactgt ggttttacca ttcttggcag tcagtaactc
     8521 aggtccagct tcaccaacaa tcgcttgtcc attgattaga tgaccgcctt gtgctaaata
     8581 aggcagtttt gaaatagaaa atgattttcc acctacacca ggaacccatt ttggtatctt
     8641 tatattattc aatccaccaa taaatccatt gatcaatcca atcatggcat taataggagc
     8701 tttccctaca gcaacaatac catcgaaaat acctccaaaa atatcaacta gaccttgcca
     8761 tgcttttgac caattccctg taaatactcc agtgataaaa tctagaaaac cactgaaaat
     8821 tcgttttcca gcattataaa aattattgaa atttgctata acaccatcga aaaatccacc
     8881 aaaataacca cttaaaaaat taaatatttc aacagctaca tctgaaacac ctttgaagaa
     8941 agaattcact ccatcatgaa accatttaac gttattatat gcccaaatta gtccagtaac
     9001 taatgcgcca atagctataa ctgctaatgc aaaccagcct cccgacattc caaataaacc
     9061 agcaagtcct tgccaaactc ctataaaact tttaacttcc ccaacgattt ttgtaacaga
     9121 tcccataagt gaaccaagaa cgattagtac tggtccaact gctgccgcta taccagcaat
     9181 tgttatgatc caattctttg tatcttgatc tagagacccc caccatttag agaattcctg
     9241 taataaggaa gttgcttttt caaaaaaagg caaaaggctt acttggactg cttcacctac
     9301 gtcagccatt gctaattttg cattgttcat agcttgatcg gcttgatcaa ttgggtcgag
     9361 agtagcatcg aatgtgtctc ctacagctcc cccactttct ccagcagttt ttgctaaatc
     9421 ttccaggttc aaagtacctc tacgaattgc atcggccatt ctaggaccgc ctttagtccc
     9481 gaatacttct gctgctgcat tgatggcttc tgtttcagaa cctgcatttt ttaccttgtc
     9541 ttgtagttct tctagtcctt gacttaaaga ttttccgtct ttcgcataag ccactgttgc
     9601 tttagataaa ctacttaaag ctgcgcttga atcaacacca gattgctcaa attgtccaag
     9661 taaagtgacc ccttcaccaa agctcaaccc taattgttta atttgtggtg cgccatcaat
     9721 tgctttttgc atcaagtcgt ctacagattg accagtattc tgcgatgttt tcgttgttac
     9781 atcaaggaca gaattcaaat catcatattc tagtccataa gcatcaatag cttgtcgagc
     9841 agatattgct gattgcgaaa catctgtatc attgatttca gcgtactgta ggagataatt
     9901 ggttgaatct tccagttttt tatccatgaa tccaaattgt gtatttacct caccaatagc
     9961 ttccccaacc gtttgtaaag gtaaatgagt atttgaacca acgtttttga aagactgtga
    10021 aagtctatca gcttgatcac ctgttgctcc ggtttttgta atgatggtat caagtgcctc
    10081 gtcaacttca ccaaatgctg caagtcctgc tgctcctgcc gctacaatag gcgcggttac
    10141 tccaacagac attttttcac caacgccttt tactttctca cctgtttctt cgattttctg
    10201 gagttttttt gctgtatcaa cagaaacgtc accttgttct ttaagtgcat cgttcgtctg
    10261 ttctaatgct gtacgaagtt tattttctcc ggtttcagaa ttgagtaatt gtttataaag
    10321 tttttctgat tcagcagaat attcaccggt ttctttgaca gatttttcat attcctctct
    10381 cagcatttga gatcgttgtt cagccaatcc taattgtttt tctaattttt gtttagccgc
    10441 tcgcaatttt tctgtttgag ttgcatcctt gtccatcgca gacacttggt ttttgtactc
    10501 ggcagcggct aagttcattt ctttgttgat ttctttgatt gttttcgaat aactgacttc
    10561 gccattcgtt ttaaaattaa gaacgacatc agattcttta ccagccattt atcttagcgc
    10621 tcctttctac caccaaggcg atttatccat agttacagat tgaggtggtt caaaatcggt
    10681 gttttgttgt aaccactgta aataagattt gagccacaag ttaggtgtag actttaaaaa
    10741 gaaactctca ctccaattca atagagtgag agcaacgtaa atataaaaac tccaaggagt
    10801 tcctatctct tccgattctt tttgtttttc tttctttttg cttgcgctta ttgaaagtct
    10861 tgtggcttct tggatttttt taggtcttcc acctgaaatg tctggctagc aaaaatttcc
    10921 atacaggtac tataagcaga caacacctct ccactcattc ccaaaaattt gaaaatagtt
    10981 tctggatctt cctctaaacc accagtacgc agcatggcat aaatcaaggc acgcatgatt
    11041 tttagatcac taggcgataa gttagcagaa gaaatttgtc cttctttttt agatagcatg
    11101 gcgttcatat cagattcaaa ttgcgaataa tctccaccat acacatctgc tataaattcc
    11161 atcgtttgca tagtaaatga aatagggaat tctgcgcctt gaataattac aacagcagaa
    11221 ttttttaagt cttcaacgtg aattccataa tcagataacc gagccattaa cccgcacctc
    11281 caactttagc caatgttttc cactgttctt catcgtatac aggttgagca atgaatttat
    11341 taaataattc tagagaagct ccgtctctat ttgaatcaaa acttgcatac atcacattat
    11401 tgtatttcag tccgatggaa accagatttg cagttacatc gtcaattttt gtttcttcct
    11461 ctgcagtggc atactcttcg tcaatcacat tggatagttg tgtattagga taccaaactg
    11521 cttttttacc gccattttca atatttccaa taaatccaaa ggcgaagtac ggaaattctc
    11581 gtgcggtatt tttgctaaac gttactccgc ttttagcaac taatcctttg atttcatcca
    11641 tcacttctat cggaataccc acgtgatcca acgcaagttc atgttttgtt tctcgcgaca
    11701 cacgacgaaa cattttgctg gatgcccatt tttctaatgc tgttccattc cctttgacac
    11761 cgatttttgt ggcaattggc aatcgtaaaa cttcactata agtcggtgca gttccaacag
    11821 aatctggcgt tgccatcatc gcaattaaaa tgtcgtccaa tccttcaaaa taaaaagtat
    11881 cttgttttcc caattaaaat cacccttccc ataaatcaag tatttttcta gtcataattt
    11941 cttcaatttt ttctttgttt tgttcatacg taccacttgc aaaatgttgg gcacgttgat
    12001 ttgttgtgcc attttcggca aagcgccagt aaaaggccgt ctcttcaaat tgaactttca
    12061 cttgatcgtt ttcaactact acttttattt gatcgcgcat atgtttcttt tttaagagcg
    12121 acttaggaat attaggaagc aactgcttta gataaaagtt agctgcttct tctaaagatt
    12181 ccaacgataa ttttttcgga tcaacttttg aaaggtttcc taaataatcc gatatttcag
    12241 aaaaaccatt cttattactt ggcattttcc acacacctca catatgtgta atagttggtt
    12301 acggtatcgt cgttctcatc accttgtata cctgtaaaat ctgaatatgg aatgccagca
    12361 ttttgcagcg cttgttcaat aacaactaaa tcctgttctg ttccaagtgt aaaaaaagag
    12421 acttggtaat aaggtaattt tttatagact ttaccggatg ccattttttt gctgttactc
    12481 acatttgagt acacaatgta tggatacatc gtccctaatc tggctttgtc tctgaacact
    12541 gctaactttg ttgatttcag cgctgttttc aattcatcaa agctaatcga cataagctaa
    12601 actcaactcc atttctcttg catcgggatt cgtataaatg cgagtaatgt tatacgttac
    12661 agaatcaatt ttgagcgcac ttaatttctc tgtgatggat ttatcccatc tgactttaat
    12721 tcgtctgaca acgtctgtct tggcttgctg tgataaatat ttttcttgag aagtcacacc
    12781 gagttcttca tagaatatta gacgcttaaa ttcgtaaatt gtagttggac gatcgtttcc
    12841 gtctgttcct gttttgatgt ctagcaattc ggctttccat ctgagattat tggtctgtct
    12901 cttcggcatt ttgaatcgct ccttgcacga taaatggcgt catggcattc atagctttgt
    12961 cgagttcatc ctctgaaact ctatattcat aggcaatgcc ggcaaccatc aaaataagat
    13021 attcttgttg gccaccagtt gctgttttga cataatcttt tgccatattt aaataaaaag
    13081 agagcaaaga atcatccatg ccctcttcaa aatgaatatg tgatttgaat ttttcctcta
    13141 aagacaattc tttagtttgc tcttccatct taaccaccaa ctggttttgt aatttcgtag
    13201 cgatatactg ccggttcaaa tggagaataa accaattgac catctagcaa gttgtaaatt
    13261 tggaatccaa tttgattttt accagagaat ttttcaacaa gtttttgaat ttccaaggca
    13321 ccaataactt cttgaatttt aaatgcagaa aaatcgccaa aatataaaac tggcgtgtct
    13381 ggttcaccct ttttatctgc tgcatctgtc caatccacag gatagccaac taattggtaa
    13441 ccaattccac cttctgcttg tgtgaatggc cgcaacaaag gaaatccatc atctgttttc
    13501 attttttcaa tggcagtcaa agcagctcga ttaataataa agcgtccctt tttcatcact
    13561 tctgtcactg gtgtattttt aaattcgatt aatgcatcat ataatttttg cccagcacct
    13621 gaagcagtta gatctaaagg ttttttaaat gctacagcct ttttggctaa tgcaccagga
    13681 ttttcatttc cagcgtcatc accattgaac atataattga tttctttacg cacataagct
    13741 tttttcaatt cttccacaac aatatcttca actggaacac cagacatttt taataatttt
    13801 ttagttactg ttgccaaagc atcgaattcg gcaggattaa gcaaaatttc atcaaactga
    13861 atagctgttt cagcaatatc agttgaacgc tctttcttgt ttacattcgc atctgctttc
    13921 ttcacaagaa ttggatattt gacatctcct gatgttcgca ccactgttcc gtatttacga
    13981 agtaaatttt cttcttgagc ataagtaata acttcagatg caattacttc tgggacagta
    14041 actgaaccgt tgccagcttc aatccctaaa gctcgagctt ctgcttcaga aatatttcca
    14101 gctacaaaat tagcaaatgc ttttcgtagt tgttgatctt tttgtttctt ggtcattttt
    14161 gcacgtgcct ccaatccgtt cttaattgat ccaagtaatc catctcgttg ctgttgagta
    14221 atcattccag aacgattttc tgaactatct tcactgtctg aatcttcttc ttgatcgttg
    14281 tcttcaccag aacggctttc gtctgaatcc gtactattcg attgatcatc tgtattgtcg
    14341 tctgtttcat cagttccaga atctgcgcct aattcgtctt taattccgtt caattcatca
    14401 ataacactgt caatttcttc tttcacggct tctaaatctg cttcgcgtaa ttctcctgat
    14461 tcaacttttt cacgtaattc agtcaatcgt tgctcacgac gtgccttcat ttttttcaat
    14521 aattctttat ccatgtattt ttcctcctac gcttctagcg cttgattaat ttttttgatt
    14581 aatttttttc tttcttcgac gtttttttct aattcatcac gacttcttaa tgcagcttct
    14641 gtatcttcgt atgctggcaa aggcacaata gaaacctcgt ataattccac ttcatggatt
    14701 gttcgaagca ttggttcaga attgtagtcc cacgtttctt cagtcggcac aaaaccaaaa
    14761 ctacattggt tgatgtctcc tcgttccata gatttaacta agtcccttgc taccgttgtg
    14821 tccggtaaat caacttcgaa tttcaaacca cgttcatctt cttcaaggcg aagggttccg
    14881 cttttagtac gtcctagcac attggaccaa tcgtgattga acaaacaacg aacatccgaa
    14941 ttactaattg cctttgagaa tgcaccagga acaatcactt cgcttaagtc atcccataaa
    15001 agtgtttgac tgttgaatac agcagcgtag ccgctaatgg tcctcgtatg agtttcttca
    15061 tcggaacgtg ttgaaagatt ggtgatgtca atcgtgcgaa tttccttctt cttcatttcc
    15121 atcacctcct ttcaagtctt gatcattcgt tggtaaggaa tcatctgttg catttttctg
    15181 gccaatccta gataagtcat ttgaaatata gatagcttgt gtttctggag tattctgttt
    15241 aggaaaacca agcatttctg ccacgttatc tggacttgta atcccggtac gaacgatgtt
    15301 gtagccaata tttgtttttg ttgagtaagg aacaaaatcc aaaatattaa ttttccattc
    15361 cactctatag ccagaattag gcataaaaaa aagagcggtg taatgttcgc tcttgttttt
    15421 caatattggt ttgattgctt tgttgtgcag atacatcatc gctttttcaa tatctgtctt
    15481 catcagcgat tgatacgtat caacattgat tcctagaaat tttcctaagt cttttttgta
    15541 aacacctaaa taattcaaaa ttgccgcgtc atcaacagga ctttttaatg tctcgatgga
    15601 atatcctttt cccaaaggaa tcatcttaac agaatgatca ctctcatttt gcgttccttc
    15661 cagttgatcc aatatagctt tgacaatttt tgtttgggcg ctattatttg gattgatgtg
    15721 ggcgtctagt ttaagcatga acgcaagtaa accgccttta gtatatttgt ccgtcaaaac
    15781 tttttcagcg ctcagaacgc cttctagagt gtttcttgca agatcaatta ttccagcacc
    15841 ttttaatgaa tcagttccga tgttcttaat gtgtcgaatc atttgaccag gtattttttg
    15901 accattcatt tcaaattctt cttgaagtcg ttcattgatt ttagttgtta caccgtacgc
    15961 caaatgaagc tgatccctat ccgttaatgg gaatgtctca ccattgatca ataaagtatt
    16021 tgtttccaat ttggtaaatt cgaatcctgt caaataatca ttgggactct tcaaaatatt
    16081 tagtaagtga tgatctttga cttcgttgcc gtcgggacca ataacaattg gtgtggccaa
    16141 tgccacctga ttagatatgt cttgaactaa ttcataaaca tcagaagatt ccatgataga
    16201 tgaatccgtc acatatcttt gaccataacg tgtgtagtgt ccaaaaatat cctcgatata
    16261 tccacgtttt tccataaaag aatagactgc attcgataac cgatcacgta atttcaattt
    16321 ttttcaccgc ctttctattt atctatagat ggaacttagg taatcatcta actcatctga
    16381 atcaatatct gtcatttgat tcatcgtttc cttatgacca cacaaaaacg ccacgaaccc
    16441 atcaatcttt ttctttgatt gacgtttact tggcgctttt tgtccattga tgttagtaat
    16501 tgctacaacg ttcaaagtgc aataaaggaa taatggatta tcaaattgaa ttcgtttctc
    16561 ataaaacaac cgttcgacat cgtcaaaagg agcattcaat actttaggat attgagcaac
    16621 ttcaacgcat tccaatccta agttttctaa tttctcaaca agtttgtcac tcatcgctgg
    16681 atcataattc acttgctgaa tatcatataa attcatgcag tcctctatgt actgcaaaat
    16741 ctgatcttga tcaatcattt ttccgtcgca aaattcaacg aaaccttgtt cagccaaatc
    16801 actgtaagga acattatctt ctttttctcg aaactctaaa tcttcattcg gaataaaata
    16861 aagttgcttt actttaagaa ccgctttacc atcttcatcc catgttggga agtttaaaga
    16921 tacacaggtc aaatctcgtg tacgtgataa atctaaaccg atataacatg gttcgccact
    16981 taaatcacca agttcttgtg tggtaactaa acatggctct acttgatcct gttcaaagaa
    17041 attatccgcg ccgttcacaa atacatccaa gtgtttcgtt agaaattcag ctttggaatg
    17101 agctgaccgt tgcgctgttt taaatgccga ttctaaagca gataaatcga ctgatattcc
    17161 ccagttagga ttgcacattt cccagacttt tctatccgtc caatcataat ttttatttgg
    17221 ctcatagatt agaacaaagt ttgaatcatt atcatcacgt ttcaacactt cttttgcttc
    17281 gcgatataca cgcattccaa cagacgacga acctttccca gcagttgaga tattaaacat
    17341 caatggctgt ggtaaagata tctgagcaga cttaaaattg tcatactgtt ccattttctc
    17401 ctgtttatgc aattcatcgt tcaatacaaa atatggattg gatccctcga tattatcaat
    17461 attcttggtt tgaacaataa atttatttga gtaagccata tcttcatgaa tatagtcata
    17521 agtaatacta gaaacagttc cctttggccc cctaaatatt ttagttcctt caagtaggat
    17581 tggattgttt aagattgtaa ctgcaaaagg tttggcagca tattgcgctt gagcaaaatc
    17641 agatgcgcaa gcatagcaat caactgataa cgccccttct ccgtacattg catatcctaa
    17701 tgaaccgaca gcaatcaatg ttttaccatt cttttttgga atctggacat aagcctcacg
    17761 agtaacgcgt acaatttggc ctttttcatt ttctttaacc caaccataaa tccaagaata
    17821 aataaatttt tcccagggtt ctaaaagaaa cggttttccc accatttctc cttttgtgtg
    17881 aacaataaat gattctaccc aatccatcat ttcattcgcg cgatctacat caaaccaaat
    17941 atcttttctt tttttccact gataccagcg atctattgct aaacgaacgg tcttaggata
    18001 cttcttaggt ttctttctta cttcttttgc aaataaatca gcataattta caccaggttc
    18061 aatcattttt cagcacctgc cttcttacgc catttatttc tgtgcgctgc tagttcatct
    18121 actggcttta cttctgggcg tgtaatttct tcatcttttc ttgcggtcga tcctccagca
    18181 attagtctac cagtttttgc tttgtttgtt aatcctaata aatctagagc tttcatcttc
    18241 ttatctgccc aagtttctac ttgttgtgcc aatggatgtt ttgattgatt agtagctcct
    18301 gatttattcg tgaatttttg cgtctccgga aatccttttt ccttccacaa taaatatttg
    18361 tgttggtaaa tttcaaaaat atccaagtat gattcgatta atggatcaag agtgatggtg
    18421 tacaaatcag acaaattcat tatttctaag atacgagctt tttcagcact tactttttca
    18481 tcaataatcg ctttacgttg cgctttagtc gtcatttttg tatacacccc cctttgtttt
    18541 ttgaaaaatt tgacctaacg atgcgcgtga ctccccgcta ccctatctcc ccacgcgaaa
    18601 aaattttgaa aatgaatagg ggggcttcaa ttgaaatacg aaggaaaaac ttttttgtct
    18661 tccgtttcgt tttcaacaat tggatgacat tttgaacata atagcatgag attgtttgga
    18721 tcaagcttaa gcagttcgtt gtctttgatt ggtacaatgt gatggacgtg tgctctcttt
    18781 ccaaatatga actgaccaca ccgctgacaa tgaccacctt ctctttcata aataaattga
    18841 cgcatatctt tccatgcttg cgttcgatag aatggtttgt tctcatgatg ataaacagac
    18901 tttgcttgct gcttcttctt gcgtgatctg cttgatctct tgtgttcagt acagtagata
    18961 ccctttgcta ttttgttcgt gcatccgtca aactgacagt atttcattct acttcacgaa
    19021 taagatttat gatatcgcct tttgcacgga tagcaccagg aatatcaata ccatgtttct
    19081 tagcatatgc acgcaattct tttgcagtca tattgtccag ttcatccgta tcttcagtgg
    19141 actgatcatt agtagcttcg tctccatcaa aattagcagc agtgtttcca tcgctatcaa
    19201 gaatttcact gctatgagct atgagttcgc cattgacagc aacaaatgat ttgccatcac
    19261 tcaaaattcc tttttcttct tttacttcaa aatttggttc ttgatctttc ggaacaacta
    19321 ctgtcctttt cttttctgaa tcccaatact ctgttccact aatggatgtt ctaattttga
    19381 tcattgccat tttaattctc tcctttgtaa tttgtacgga ttacttttgc gcccattcgc
    19441 tcataccatt caatcgtttc ctgcagattg ggtagagtgt gtgacaataa agaaataact
    19501 aacgtattca ttccagttct gtaatcatca ttctccactt gcacatgtgc atgattacca
    19561 ttccaatttt ctctgtacgg actattctct gcttcaattg ctttcttata aactttagcc
    19621 aaacttgatt ctacttcaat actaagtacc gcttcatgaa aatcattcat ttgtttactc
    19681 tcctatcaga tattttgtgg tatgatttat aaaaaagatg cgaggtgttt taatgcttca
    19741 agaagttaat attaagtatc gtgatcgtca aaatacagag acaatttatt tgccaaatag
    19801 ttgtccgcat tgcggaaata taatgactcc gtatgtgtat tttggggttt catcatatag
    19861 tgcgttcgat aatgatagaa tctttggcgt attagtacag tgtataaatg atgagtgcaa
    19921 caaattttat agtttacaat ttataaatac gtttaatgaa gatgaaggat attctgaaaa
    19981 tattttaatt aaagattatt accgtccgcc gattaaaata gatttaccag aaaatattga
    20041 aaaagtttct cctacatttg ttgatattta tactcaagcc acacaagcta aatctgagca
    20101 attggaacaa attgctggag ttggttatag aaaagctcta gaatttctaa ttaaagatta
    20161 tgcaatatct aaaaattcag aggatgaaga taaaataaaa aaaatgttat taggtaaaac
    20221 aattgaaaat tatttgaatg attttcctaa actccaaaga ttagccaaag ctgctacttg
    20281 gattggtaat gatgaaacgc actatgttcg tagacatact aataaagatg ttcatgatat
    20341 ggatgcattt attaaatcag cagcgcaatt tatcgcggct gattatgatg ctgatttagc
    20401 tgatcaattt ataaatggaa ataactttat gccaactact aattaaaaga ataaaagagc
    20461 cacagatacc aattaataaa acggttggga tatcttggct ctttcatatt tttttgacac
    20521 taatagaata tcatgctgct aacaatgttt acatagctac aatgaccgaa catttagtga
    20581 acagtgtaat tttagaataa agagagttgt tctttgtact tctcgctatt gtaaaaatca
    20641 atttcgtttt cttttttcaa tcggtcagct tgtctgtgtt catactcatc taaaaagttc
    20701 aatgtctttc taatttctgc atgtcgctgt ctgacatacg atgagctata cccaacagat
    20761 tcagcaatat cttctaagct catttgatca acatatttca tcttaacaat ttgattatcg
    20821 ataccagaaa agctatctat cagcaataac atttcttctt tttgctcgat caacaattcc
    20881 aattcatttt ctatcttttg gatattctct tctaaagaag aagttctcga attcttttcg
    20941 atgcggacgt ttgctaaatc accacagacc catcgattca attcaagctt acttttatta
    21001 agattccact ttaagtaaag aatctgctca tctagttctt ggtaatcttt taaccaatga
    21061 aatctcacaa acgccacccc ttatggtaga atagtattgc gctgctatct cgaaaggggt
    21121 agctttttta ttttttctta gccgttccat tctggattgc cttttgttcc aaacgtcgtt
    21181 ttttcttctt aatctttgat tttgtttttc ccatacaatt ctcctttccg aaaacacatt
    21241 ttgttcgatt tttaatacta aataaaagtt ggccagaatt tttcgttcgt ctttactcat
    21301 ctttttttga ataagattac tagcataaaa atagttagta ccacgaaaat gaacaggtaa
    21361 atcaattacg ttcaccttct tcatgccagt tatcagccac tagaatcttt gcgttagtgc
    21421 cgccaccgtt ttcttttttg tatttttcaa atgctttata agcagctgtt tttgatttat
    21481 agatttttcc ggaaccgact gggaacatcc atgtgccctc tgtgatagct aagatgtata
    21541 ctttcattta attcacccct ttaatcccca acactacata tccggacttc tgcatgtaat
    21601 cagtaatata agtgatctct gctttgtatg agtcaccagt gtaactaaat gttggtctcg
    21661 ttagtccagg taattcatgt ttaatgtatt ttttatattc acgtagtatt aactgatcac
    21721 caactttgaa gtcacgatca ttcttgcgga tctcgaattg tttgcgtccg ctcgtcactg
    21781 cttcaaaata ttctggtagt atttttagtt cgtggatcat tctgtttcct ccttcaaaat
    21841 tcgctcatca tcaaaaagtg aatctccaca aaatccttct cccaaatatt tatcgttcat
    21901 gtataagtca accaaatatt tgtgatctgc ttctcgatat tcattaatat taacgatctt
    21961 tattttgtaa acattatctt cgtcgctcac tatcttgaat tgttctctga ctttcaccac
    22021 cgttcctcct ctttttgaaa aaattttagt gtttttgtaa gaccccctaa atcagctttt
    22081 ttatttgata taatacttgt atcgattaat caaatcgaaa ttaaatttaa aaaggtgatt
    22141 tatcatgaat atcaacaatt tagtaaaact cgttgaacaa cgttttaaag aatcaggatt
    22201 agaacaatta aaaaaagata attctccaga attattttct aattttaccg aagacgacat
    22261 tcctactctt aaacaattaa aatattattc tgattattgg acgcaaactc gtgagaatga
    22321 attaaattta gaaaaactca ttattaaaaa gaaatagcaa ctccagccca cacggctggt
    22381 tttttagttt caacttccct ccttacgggt caaaatcatc ggttccggca taatctgatc
    22441 atcctttagc aacaaatcag gatatttttt ccttagcttc ttattgttcg tgtaagtttt
    22501 caatagccat tggttggctt cgcttttgct ttctccagaa aaataaattt tccctgtttg
    22561 gatgcctgca agttgaatca tcattttcac ctcaaatcaa atttttttct gctagatgga
    22621 gcaaaagcaa tccgacatca agaatctttt gacccattat ttcagctact ttgctcggct
    22681 taactccttg agaaaagagc aagcgtgctt gctctatctc ttcgtgagtc caaataaatt
    22741 tggcttcctc aaggataatt actggatcag ttgctctcat tgttcctctg gaaaaatgat
    22801 gtcattctta tgcttggacc aggtatctgc atatggaaca aagtattgtc tagctaattc
    22861 gatttgatca attaaagcag tttctgataa ttcatggtct gcagcaattt cattcatact
    22921 atcgccttca tctaaacaga taagaccgtt gacgttatcg atttttacat ttgtgactgc
    22981 ttgactttct ccacgctcat aatattctac agttgatact gatacatcag cttcggagtt
    23041 taattgcaga atctgttact gccgggataa tttaatattc ttttcataat tcaccttttt
    23101 tcctactgac atcgaatatt tttcatgtta tgattagccg tgaaaggaga gctatattat
    23161 gtgtttacac atgatgtcct tagctcattc ggttctttgg aagctttttt ggaaataact
    23221 ctcaggttct tgacgcaaat ctcatttatt ctttgaaaca aaaaacttcc attttccgag
    23281 agctttggat cacttcaaaa gagatttgca tttatatttc tgtgaccttg agtcgcgtaa
    23341 agaaccttac agcaatggcg gcaattccac tttcaggaaa ccgccttttt tcattgatat
    23401 tggtggttag cggactaccc attttctttc ggcagtgcca aaccacaaat gatctgtgtt
    23461 gatgtttagg tagttgtata ccagataagc taaatccaaa gtgcctgcat cttcaagacg
    23521 acaatattcc aaaaattctc ccatagctgg acataaaaat cctggagcta tcatatccca
    23581 acagttaata gtgcaaagaa attcgtttag cttctgaacg ttttctttgg tttcacgaat
    23641 tacaacatac tccaattctt ctccgctggc agagcattcc aaaacttcaa attgattaag
    23701 aaatggtaga acatatgctt caaacatcaa cattccatct ttctgttttt gcacttgttt
    23761 tgcatcaatc atgtctatcc tcctattcag ttatttcttc cattaatgga attatttcag
    23821 gtcactggac ttaacgaaaa caccattgac catctttcca gtccgacctt tgatttcgtc
    23881 ataagcaaaa tccaagcact cgtataaatc catatcattt tgcattgcca agatgatcaa
    23941 ggttactact acatcgccaa ttccgtctct taaaccatgt tcatcttttc tagctagaga
    24001 agcggcaact tccccgattt cttcgaccgt ttttaacatt tgcttgctga aatccgcttg
    24061 atccaatccc ttatcttttg cccactgctc tactttcgcg attagttcgt ccattatttc
    24121 tcctccacat acctaaactg tcttcctttt gaatcaatcc ataagctcct agctctatcc
    24181 caaataatat ttttgctcag accagtaatt tcagataact gtttagcggt acccgttact
    24241 agaattcgat caccatgcca gattgcaatc tttctcggcg ttctccgttt gggcttttca
    24301 gtccacattg atttaccgag cttttggact tctgcaacta tttttttatc ttcctgccaa
    24361 tcttctgaat aagtcaattc gataattcgc tgcattgccg ctttcttatc catcccgaca
    24421 ttctcctttc agtaatttga gtacttgatc aagtgcgctc tcacgtccgc catgaaatgt
    24481 gttgagccac ttgtcttcgt acgacacact ttgtcttaaa gcttcttgat gcattaattc
    24541 gatctgtgct gtaaatgtct ttaggtccat cttgtttaca cctgctcaag ttcattaaga
    24601 tgtttttgca agcctttaac acaatcaatg aatagtaatt tggtatatgc taaatttctt
    24661 aattgcgttc catcgatata gagtgcgaaa tagtatctga gtttactcca actcgaacga
    24721 tcattcttaa ttccttcaat tccagcttct tcgagttgat catatacgtc tctcaggacg
    24781 tctaattcct cgcccttttt ataattcgct atttcattaa ttagttctag ataatcgatc
    24841 ttcatttttc cacctcttag aatggtgctt ttgattgtct attagctcgt tctagcgctt
    24901 ttttcttgag ataggcttct tggtcgattg cccattcagg aagcttctct cgtcttcctg
    24961 tgcgcttgta tccactgctt gcgttcttag gttcactttt ttctttcctt gcccaacttc
    25021 gaatagttgc caaatagttt ttataagtct taccagatga ttcacaatac tctgacagtc
    25081 gttcgattcg ctcttggtaa tcattaggga attctgtttt gagtttctcc atctgctcat
    25141 ctgacaaaag aacattttta tactctccgt atttatgacg gacgggctta gccttcgatt
    25201 ttttcgaagg cggtaactct ctt
//
LOCUS       DACCVU010000034        23390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCVU010000034 DACCVU010000000
VERSION     DACCVU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.034"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(43..2187)
                     /locus_tag="IVW29_001221"
     CDS             complement(43..2187)
                     /locus_tag="IVW29_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7838713.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="IVW29_001222"
     CDS             complement(2544..3917)
                     /locus_tag="IVW29_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7838714.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="IVW29_001223"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="IVW29_001223"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7838715.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="IVW29_001224"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="IVW29_001224"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7838716.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="IVW29_001225"
     CDS             complement(5365..6378)
                     /locus_tag="IVW29_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838717.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="IVW29_001226"
     CDS             complement(6459..7058)
                     /locus_tag="IVW29_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7838718.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="IVW29_001227"
     CDS             7244..7945
                     /locus_tag="IVW29_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838719.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="IVW29_001228"
     CDS             7955..10666
                     /locus_tag="IVW29_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7838720.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="IVW29_001229"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="IVW29_001229"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7838721.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="IVW29_001230"
     CDS             complement(12805..13986)
                     /locus_tag="IVW29_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7838722.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="IVW29_001231"
     CDS             14238..15761
                     /locus_tag="IVW29_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7838723.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="IVW29_001232"
     CDS             complement(15830..16558)
                     /locus_tag="IVW29_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838724.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="IVW29_001233"
     CDS             complement(16571..17851)
                     /locus_tag="IVW29_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7838725.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="IVW29_001234"
     CDS             complement(17992..18594)
                     /locus_tag="IVW29_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7838726.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="IVW29_001235"
     CDS             18839..19045
                     /locus_tag="IVW29_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838727.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="IVW29_001236"
     CDS             19357..20076
                     /locus_tag="IVW29_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7838728.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="IVW29_001237"
     CDS             complement(20157..20840)
                     /locus_tag="IVW29_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7838729.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="IVW29_001238"
     CDS             21058..23244
                     /locus_tag="IVW29_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7838730.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7781 a         4211 c         4590 g         6808 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataaa ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
//
LOCUS       DACCVU010000035        23241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCVU010000035 DACCVU010000000
VERSION     DACCVU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.035"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..637)
                     /locus_tag="IVW29_001239"
     CDS             complement(59..637)
                     /locus_tag="IVW29_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7838731.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(714..1244)
                     /locus_tag="IVW29_001240"
     CDS             complement(714..1244)
                     /locus_tag="IVW29_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7838732.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1248..2108)
                     /gene="rlmB"
                     /locus_tag="IVW29_001241"
     CDS             complement(1248..2108)
                     /gene="rlmB"
                     /locus_tag="IVW29_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7838733.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2101..2496)
                     /locus_tag="IVW29_001242"
     CDS             complement(2101..2496)
                     /locus_tag="IVW29_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7838734.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2493..3905)
                     /locus_tag="IVW29_001243"
     CDS             complement(2493..3905)
                     /locus_tag="IVW29_001243"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7838735.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3902..4438)
                     /gene="cysE"
                     /locus_tag="IVW29_001244"
     CDS             complement(3902..4438)
                     /gene="cysE"
                     /locus_tag="IVW29_001244"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7838736.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4760..6220)
                     /locus_tag="IVW29_001245"
     CDS             complement(4760..6220)
                     /locus_tag="IVW29_001245"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7838737.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6390..7547)
                     /locus_tag="IVW29_001246"
     CDS             complement(6390..7547)
                     /locus_tag="IVW29_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7838738.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7581..8951)
                     /gene="radA"
                     /locus_tag="IVW29_001247"
     CDS             complement(7581..8951)
                     /gene="radA"
                     /locus_tag="IVW29_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7838739.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9014..9541)
                     /locus_tag="IVW29_001248"
     CDS             complement(9014..9541)
                     /locus_tag="IVW29_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7838740.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9771..10577
                     /gene="proB"
                     /locus_tag="IVW29_001249"
     CDS             9771..10577
                     /gene="proB"
                     /locus_tag="IVW29_001249"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7838741.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10574..11821
                     /locus_tag="IVW29_001250"
     CDS             10574..11821
                     /locus_tag="IVW29_001250"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7838742.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11897..12241
                     /locus_tag="IVW29_001251"
     CDS             11897..12241
                     /locus_tag="IVW29_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838743.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12358..12684)
                     /locus_tag="IVW29_001252"
     CDS             complement(12358..12684)
                     /locus_tag="IVW29_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838744.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13299..14501)
                     /locus_tag="IVW29_001253"
     CDS             complement(13299..14501)
                     /locus_tag="IVW29_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7838745.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14618..15133
                     /locus_tag="IVW29_001254"
     CDS             14618..15133
                     /locus_tag="IVW29_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7838746.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15332..15778)
                     /locus_tag="IVW29_001255"
     CDS             complement(15332..15778)
                     /locus_tag="IVW29_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7838747.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15825..17303)
                     /gene="msr"
                     /locus_tag="IVW29_001256"
     CDS             complement(15825..17303)
                     /gene="msr"
                     /locus_tag="IVW29_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7838748.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17657..18286)
                     /locus_tag="IVW29_001257"
     CDS             complement(17657..18286)
                     /locus_tag="IVW29_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7838749.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18270..18734)
                     /locus_tag="IVW29_001258"
     CDS             complement(18270..18734)
                     /locus_tag="IVW29_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7838750.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19221..20489)
                     /locus_tag="IVW29_001259"
     CDS             complement(19221..20489)
                     /locus_tag="IVW29_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7838751.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20720..21457)
                     /locus_tag="IVW29_001260"
     CDS             complement(20720..21457)
                     /locus_tag="IVW29_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7838752.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21524..22093)
                     /locus_tag="IVW29_001261"
     CDS             complement(21524..22093)
                     /locus_tag="IVW29_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7838753.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22221..23093
                     /locus_tag="IVW29_001262"
     CDS             22221..23093
                     /locus_tag="IVW29_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7838754.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         6549 a         4952 c         4203 g         7537 t
ORIGIN      
        1 ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt tttctaaatt
       61 attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc ccgtaagcgc
      121 tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa tcaacgcctt
      181 gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg caattttcga
      241 ttgcttcttt taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg atctctgcta
      301 aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct ttttgatcac
      361 gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt ataacgcttt
      421 tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat gcctgatata
      481 aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa taataggtgt
      541 ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat gcttcttcgt
      601 caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc cataaattcg
      661 tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat atgttattta
      721 ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc ttcattccat
      781 ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc tattgttttt
      841 tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc tccttgggaa
      901 aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt caaacggtca
      961 ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc tttcgtaaaa
     1021 acaacatcca actggtattt cttataattt tgttggatgc ccggaactag ttgggcatcg
     1081 aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg atgtaaaaga
     1141 gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc cggccacgaa
     1201 ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta ctccccctga
     1261 cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt taagctctgg
     1321 acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa tcctggactc
     1381 attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt ccatttttgg
     1441 aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt gagctgttgc
     1501 acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc cccagtggaa
     1561 gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat cacaccatcg
     1621 acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc ttcgatgcta
     1681 tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat cagttggtca
     1741 agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac gccgtgatcg
     1801 ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg ttcacgtgcg
     1861 atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa gagtttattc
     1921 cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa attatcttct
     1981 aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt tttatttgaa
     2041 tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt tgttctttct
     2101 ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt ccatccgctc
     2161 ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg cgacccgata
     2221 cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc ctctgcgata
     2281 ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa tcaaaaaggc
     2341 ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg gttttgtttc
     2401 tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc caacataagc
     2461 caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca tcgggttcct
     2521 tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat ttcgtcgctt
     2581 cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg ttcaatctct
     2641 tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca ttgcgagaat
     2701 aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag ataagtgttc
     2761 agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa atcatcgtcc
     2821 atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc cgcatcatct
     2881 gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt attcttcagt
     2941 cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat cggacgacgg
     3001 tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt gatcatttca
     3061 tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc gccaatcgtt
     3121 acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc ttcactttgt
     3181 gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg gatatcgatc
     3241 gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc gggacgtcct
     3301 ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca taaagcgaaa
     3361 tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc tccgacttcc
     3421 agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg ggtacggtaa
     3481 tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg aataaatgct
     3541 aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggttc gacattcaac
     3601 gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg tgctgtaata
     3661 cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt cgatacgaaa
     3721 tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc aatggtgcta
     3781 cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata cataccgaca
     3841 tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt gtagattttt
     3901 atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct ggtactccta
     3961 ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg attttggcgt
     4021 tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg gctccttgtt
     4081 taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt acgccatgga
     4141 ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc attccatggt
     4201 cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca gttaggaaac
     4261 gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat aaaaaatgag
     4321 agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc tcagctgtcg
     4381 aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac caactcatga
     4441 ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa agaaaagaag
     4501 cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca cttttcttca
     4561 taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta ctttgtttcc
     4621 tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga ggcatttcgg
     4681 caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc agctgcttac
     4741 ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat gatttttcac
     4801 gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct gatacagcta
     4861 cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga acagctttga
     4921 tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt gctttgaatg
     4981 cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct tcattcaata
     5041 caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca tagctcattt
     5101 gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca tctgggtttt
     5161 ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat gcatctagat
     5221 ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg aaagctgcgg
     5281 gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt tcgaagattt
     5341 cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt gcatctggta
     5401 gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca cgtttgctta
     5461 gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt ggtggtgtcc
     5521 agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg tcatctccac
     5581 gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa ttataagttg
     5641 gcataccatc gcgtttttgg atgacaaagt cgccaccgat attatcggat tcgaacgaaa
     5701 tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca cggaaacgta
     5761 caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc aagttcgcac
     5821 atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt tctgcttcta
     5881 attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc tgatcaatca
     5941 gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct ccaggatttt
     6001 caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg cttttttcgc
     6061 catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag tctccatcat
     6121 tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata tgcaggtgcc
     6181 cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc ttctttcaaa
     6241 ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata tcatttattt
     6301 aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca actggttgaa
     6361 tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt tttctctgtc
     6421 tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat catgcgtcca
     6481 gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat gtaatgctgt
     6541 ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg ttggcgttct
     6601 gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac tggttttacg
     6661 gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga gactttattc
     6721 aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt gatcaatttg
     6781 ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga tataccctct
     6841 tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg tttcaaacta
     6901 tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat catcaaggtt
     6961 ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat cacgcttgta
     7021 tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag ttgactctca
     7081 acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac tttcttccat
     7141 tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat catcagtaaa
     7201 atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt cgagatcaca
     7261 acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata agtcaagctg
     7321 acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt ggccaacagc
     7381 aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt attattcagc
     7441 catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga aatacccaag
     7501 cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt ttttcctccg
     7561 tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc caaagttgct
     7621 actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt tttaggtagg
     7681 tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat atgactgaca
     7741 cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg ctgtgttcct
     7801 ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc tggttcattt
     7861 aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag caacccagct
     7921 cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc tgttgttgtt
     7981 cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac caaaattgga
     8041 cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag gcgttcttct
     8101 aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat ttcaaaaatc
     8161 ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg aaaactatga
     8221 tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat tcttgggccg
     8281 gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc atttgtttta
     8341 gcggttttga gtagttctgc agtggtttct cttacttggc tgacacttcc tgccacactt
     8401 gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc aggggagata
     8461 tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata aaattctgtg
     8521 ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc ttcgccggat
     8581 acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa cagcaaagtg
     8641 gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac cacacctccg
     8701 cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt tttagggacg
     8761 acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg agttctgcgg
     8821 tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca atttgggcag
     8881 cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg tacttttgat
     8941 ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat caagcttaag
     9001 tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga gcagtcgttt
     9061 gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc tcgccttttt
     9121 tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg ccttcatttg
     9181 cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac actaaaaacc
     9241 gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct cccatgtaag
     9301 ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct gatacatctt
     9361 ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc cagatacttg
     9421 gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt gtagcgcgct
     9481 gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct ctttttctca
     9541 ccggttttcc tccctaaata aactactcct attctaacat atccgaggct gtaaaagact
     9601 ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa ttcgttcttt
     9661 ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta ctcgtcaaaa
     9721 caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac atgcgtaaag
     9781 aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg atttatccca
     9841 atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct gatcttagaa
     9901 atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt atgcataaat
     9961 tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg gctgttggtc
    10021 aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa cagacggcac
    10081 agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat gtgaggaata
    10141 cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac gatactgtag
    10201 cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg tcagccatca
    10261 tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac gggttttatt
    10321 cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat aaaatcgatg
    10381 aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc ggtatgcaga
    10441 gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt ttagcaaatg
    10501 gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt actttattta
    10561 aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag atagcgcgca
    10621 acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa tggcacaaac
    10681 catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag aaaaagcagt
    10741 cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg agcgaatcaa
    10801 tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg gaaaagtaga
    10861 tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc cactaggcgt
    10921 gatcggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta gtctagcatt
    10981 caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct cgaatcaatt
    11041 attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt atgccatcca
    11101 gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga cagaatactt
    11161 agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag aacaggcaac
    11221 ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata aagaagcaca
    11281 gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat ctgtctgtaa
    11341 cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc cagctattga
    11401 aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt tagaatatct
    11461 agagacggct gctttagcag aagacacaga ttgggatact gaatatctag attatatcct
    11521 tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc ggcataatac
    11581 taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt tcttaaatga
    11641 agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg gatcggtatt
    11701 tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg gtccaatggg
    11761 gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa tccgcgaata
    11821 atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg ttctatccaa
    11881 ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc ttatttctct
    11941 gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat tcaaaaaacg
    12001 aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg ggcaaaaagg
    12061 gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag cgacaatcaa
    12121 ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga ttgctgggat
    12181 ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga ctttccgcta
    12241 aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg ccggactttt
    12301 tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg cgttcgccta
    12361 ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt caaatgtttc
    12421 gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt ctttttcttc
    12481 ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag ctattttttg
    12541 ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt tgatatagcc
    12601 acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg tcaacccgac
    12661 ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta aatgaatttt
    12721 agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg aatgtgacaa
    12781 aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac tcctcaatca
    12841 taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc ttgtgattca
    12901 gagttaaacg acagtctcac aacctcttgg taaacggcgt tcaatcagct gaccctcggt
    12961 attagctcaa aaaacggtaa aatatgagaa gctatttttc cgttttgttt ctctaatact
    13021 taggataaaa cgctgatttt acaacctctg ttaaacggtg ttcaatcagc tgtccttcgg
    13081 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
    13141 caggacaaaa cgctgatttt acaacctcct gataaacggt gttcaaaagc ttgcatctgc
    13201 ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatg
    13261 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
    13321 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
    13381 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
    13441 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
    13501 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
    13561 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
    13621 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
    13681 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
    13741 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
    13801 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
    13861 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
    13921 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
    13981 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
    14041 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
    14101 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
    14161 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
    14221 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
    14281 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
    14341 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
    14401 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
    14461 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
    14521 tcatacatga tttattttac atgtcaatat gcacatgtaa aacgtggaaa ctttactgtg
    14581 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
    14641 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
    14701 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
    14761 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
    14821 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
    14881 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
    14941 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
    15001 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
    15061 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
    15121 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
    15181 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
    15241 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
    15301 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
    15361 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
    15421 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
    15481 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
    15541 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
    15601 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
    15661 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
    15721 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
    15781 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
    15841 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
    15901 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
    15961 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
    16021 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
    16081 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
    16141 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
    16201 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
    16261 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
    16321 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
    16381 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
    16441 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
    16501 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
    16561 tgtttagagg aagacaaccg atccagtctg atttgttgct ttttcttttt actggacact
    16621 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
    16681 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
    16741 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
    16801 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
    16861 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
    16921 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
    16981 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
    17041 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
    17101 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
    17161 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
    17221 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
    17281 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
    17341 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
    17401 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
    17461 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
    17521 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
    17581 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
    17641 agtaactgaa aaccttttat tcgaacatag tgatcttttc tggtgctaaa cgaacagact
    17701 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
    17761 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
    17821 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
    17881 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
    17941 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttgtagattt
    18001 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
    18061 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
    18121 cagcgaccac aaagcgccat ggtttcatat tgacagaaga aggtgcagtc gtcgcttctt
    18181 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
    18241 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
    18301 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
    18361 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
    18421 atttcacaat gcttgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
    18481 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
    18541 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
    18601 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
    18661 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
    18721 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
    18781 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
    18841 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
    18901 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
    18961 gcagtagata attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
    19021 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
    19081 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
    19141 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
    19201 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
    19261 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
    19321 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
    19381 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
    19441 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
    19501 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
    19561 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
    19621 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
    19681 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
    19741 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
    19801 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
    19861 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
    19921 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
    19981 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
    20041 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
    20101 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
    20161 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
    20221 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
    20281 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
    20341 ctttctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
    20401 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
    20461 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
    20521 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
    20581 tgtttctttt taataaattt gctttttctg cataaaaaga cgtaaatgaa gaatattctt
    20641 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctctct
    20701 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttgctga
    20761 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcagatg
    20821 ttaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
    20881 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
    20941 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
    21001 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
    21061 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
    21121 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
    21181 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
    21241 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
    21301 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
    21361 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
    21421 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
    21481 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
    21541 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
    21601 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
    21661 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
    21721 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
    21781 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
    21841 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
    21901 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
    21961 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttccgct tggatctgat
    22021 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
    22081 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatgatg
    22141 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
    22201 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
    22261 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
    22321 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcga ggctcccgag
    22381 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
    22441 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
    22501 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
    22561 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
    22621 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
    22681 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cggaatcatc
    22741 ctttagcaaa gcaaacagtc atccatccgc aggaactgaa aactgttcct ttgatcattt
    22801 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
    22861 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
    22921 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaagtattca
    22981 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
    23041 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
    23101 ttttttcctt cgctttcagc tttttaacaa cgaataagtt aagcaaaaga tccaatgatt
    23161 aaataacata agtttcttaa tttgttcctt ccttttattt ctgataattc cagtaaatta
    23221 ctataattat aaaaaaagaa t
//
LOCUS       DACCVU010000036        22961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCVU010000036 DACCVU010000000
VERSION     DACCVU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.036"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            144..1337
                     /locus_tag="IVW29_001263"
     CDS             144..1337
                     /locus_tag="IVW29_001263"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7838755.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1467..2939
                     /locus_tag="IVW29_001264"
     CDS             1467..2939
                     /locus_tag="IVW29_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7838756.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3022..3810
                     /locus_tag="IVW29_001265"
     CDS             3022..3810
                     /locus_tag="IVW29_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7838757.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3877..4545)
                     /locus_tag="IVW29_001266"
     CDS             complement(3877..4545)
                     /locus_tag="IVW29_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7838758.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4660..6372
                     /locus_tag="IVW29_001267"
     CDS             4660..6372
                     /locus_tag="IVW29_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838759.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6375..8144
                     /locus_tag="IVW29_001268"
     CDS             6375..8144
                     /locus_tag="IVW29_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838760.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8349..8915)
                     /locus_tag="IVW29_001269"
     CDS             complement(8349..8915)
                     /locus_tag="IVW29_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7838761.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(8915..9871)
                     /locus_tag="IVW29_001270"
     CDS             complement(8915..9871)
                     /locus_tag="IVW29_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7838762.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9871..10527)
                     /locus_tag="IVW29_001271"
     CDS             complement(9871..10527)
                     /locus_tag="IVW29_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7838763.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            10897..12915
                     /locus_tag="IVW29_001272"
     CDS             10897..12915
                     /locus_tag="IVW29_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7838764.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13342..15756
                     /locus_tag="IVW29_001273"
     CDS             13342..15756
                     /locus_tag="IVW29_001273"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7838765.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16104..17657
                     /locus_tag="IVW29_001274"
     CDS             16104..17657
                     /locus_tag="IVW29_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7838766.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18038..19822)
                     /locus_tag="IVW29_001275"
     CDS             complement(18038..19822)
                     /locus_tag="IVW29_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838767.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19837..21558)
                     /locus_tag="IVW29_001276"
     CDS             complement(19837..21558)
                     /locus_tag="IVW29_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838768.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21658..22419
                     /locus_tag="IVW29_001277"
     CDS             21658..22419
                     /locus_tag="IVW29_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7838769.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7315 a         4105 c         4732 g         6809 t
ORIGIN      
        1 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
       61 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      121 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      181 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      241 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      301 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      361 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      421 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      481 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      541 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      601 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      661 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      721 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      781 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      841 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
      901 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
      961 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1021 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1081 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1141 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1201 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1261 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1321 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1381 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1441 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1501 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1561 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1621 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1681 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1741 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1801 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1861 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     1921 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     1981 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2041 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2101 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2161 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2221 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2281 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2341 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2401 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2461 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2521 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2581 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2641 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2701 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2761 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2821 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     2881 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     2941 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3001 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3061 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3121 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3181 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3241 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3301 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3361 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3421 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3481 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3541 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3601 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3661 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3721 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3781 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3841 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     3901 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     3961 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4021 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4081 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4141 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4201 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4261 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4321 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccattat
     4381 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4441 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4501 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4561 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4621 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4681 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4741 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4801 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4861 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     4921 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     4981 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5041 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5101 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5161 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5221 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5281 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5341 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5401 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5461 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5521 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5581 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5641 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5701 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5761 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5821 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     5881 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     5941 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6001 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6061 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6121 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6181 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6241 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6301 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6361 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6421 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6481 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6541 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6601 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6661 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6721 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6781 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6841 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     6901 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     6961 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7021 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7081 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7141 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7201 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7261 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7321 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7381 aaccagaaaa ctgtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7441 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7501 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7561 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7621 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7681 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7741 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7801 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7861 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     7921 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     7981 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8041 tcttgcgtga tggggaagtc attgaagaag gcagtcatca cgagttatta gcagaaaatg
     8101 gattttatgc ggaactttat cataatcaat ttgtatttga ataaagaggt tgtgaaaaag
     8161 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgattt
     8221 ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga ggttatgaaa
     8281 tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt atgttttgcg
     8341 ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt cttttggttg
     8401 ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca aaaagaatca
     8461 accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag gatcaatcgt
     8521 ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt ctctggcata
     8581 gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc tgctttgaga
     8641 ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa caatgagaca
     8701 cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc ttgttgctgg
     8761 atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa aaccgtatcg
     8821 tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat ttcttgcagt
     8881 aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt ttccgtacat
     8941 catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca ttcaagactt
     9001 cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca tcccctgtca
     9061 aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg acattgacat
     9121 agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac attctcgttt
     9181 tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct aaaatggtgc
     9241 gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg atcaaatgcg
     9301 tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa gtttgataat
     9361 catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat ccaagttcta
     9421 cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca tctggcagac
     9481 aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact acttgctcaa
     9541 atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg aaataaacga
     9601 tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc tggaattgga
     9661 ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca ctgcaaaaag
     9721 tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct ccatcgatcg
     9781 gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa gaatgatatc
     9841 ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt aagagttatc
     9901 tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag aaagactgag
     9961 taagtaaccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc ggcttatccc
    10021 gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg ttcttttttg
    10081 aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc ctgctgcatg
    10141 tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag gacgctctcg
    10201 catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa atccagaaac
    10261 gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg cacaagcaaa
    10321 taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt agctgttcag
    10381 atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag aaagccacga
    10441 cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga agctaccagc
    10501 cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg acttattata
    10561 gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg attcctcttt
    10621 gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt tttggtaatg
    10681 caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa acaagaacgt
    10741 agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat tacaatatga
    10801 ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga agttataatt
    10861 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
    10921 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
    10981 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
    11041 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
    11101 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
    11161 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
    11221 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
    11281 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
    11341 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
    11401 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
    11461 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
    11521 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
    11581 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
    11641 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
    11701 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
    11761 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
    11821 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
    11881 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
    11941 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
    12001 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
    12061 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
    12121 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
    12181 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
    12241 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
    12301 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
    12361 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
    12421 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
    12481 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
    12541 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
    12601 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
    12661 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
    12721 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
    12781 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
    12841 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
    12901 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
    12961 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
    13021 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
    13081 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
    13141 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
    13201 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
    13261 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
    13321 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
    13381 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
    13441 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
    13501 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
    13561 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
    13621 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
    13681 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
    13741 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
    13801 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
    13861 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
    13921 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
    13981 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
    14041 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
    14101 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
    14161 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
    14221 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
    14281 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
    14341 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
    14401 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
    14461 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
    14521 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
    14581 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
    14641 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
    14701 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
    14761 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
    14821 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
    14881 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
    14941 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
    15001 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
    15061 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
    15121 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
    15181 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
    15241 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
    15301 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
    15361 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
    15421 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
    15481 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
    15541 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
    15601 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
    15661 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
    15721 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
    15781 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
    15841 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
    15901 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
    15961 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
    16021 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
    16081 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
    16141 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
    16201 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
    16261 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
    16321 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
    16381 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
    16441 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
    16501 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
    16561 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
    16621 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
    16681 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
    16741 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
    16801 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
    16861 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
    16921 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
    16981 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
    17041 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
    17101 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
    17161 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
    17221 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
    17281 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
    17341 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
    17401 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
    17461 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
    17521 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
    17581 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
    17641 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
    17701 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
    17761 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
    17821 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
    17881 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
    17941 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
    18001 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
    18061 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
    18121 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
    18181 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
    18241 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
    18301 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
    18361 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
    18421 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
    18481 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
    18541 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
    18601 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
    18661 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
    18721 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
    18781 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
    18841 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
    18901 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
    18961 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
    19021 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
    19081 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
    19141 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
    19201 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
    19261 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
    19321 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
    19381 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
    19441 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
    19501 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
    19561 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
    19621 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
    19681 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
    19741 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
    19801 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
    19861 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
    19921 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
    19981 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
    20041 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
    20101 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
    20161 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
    20221 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
    20281 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
    20341 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
    20401 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
    20461 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
    20521 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
    20581 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
    20641 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
    20701 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
    20761 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
    20821 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    20881 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    20941 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    21001 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    21061 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    21121 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    21181 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    21241 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    21301 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    21361 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    21421 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    21481 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    21541 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    21601 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    21661 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    21721 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    21781 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    21841 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    21901 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    21961 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    22021 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    22081 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    22141 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    22201 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    22261 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    22321 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    22381 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    22441 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    22501 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt
    22561 taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa
    22621 gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga
    22681 gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat aaattaaata
    22741 ggtgcataaa agcattacac ttaaaataca ttgttgctta cattattaca atatgttaat
    22801 aaggcaaaaa taaatagatg gattgaataa acatattttt taatcaaata gtccctactg
    22861 tgactttcct cagctatcgg tgatactacc gatagcattt ttttgcttat aaaataatta
    22921 taagtattta gagatttttc ttctaacatt cttcaaacaa g
//
LOCUS       DACCVU010000037        22573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCVU010000037 DACCVU010000000
VERSION     DACCVU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.037"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            118..2559
                     /locus_tag="IVW29_001278"
     CDS             118..2559
                     /locus_tag="IVW29_001278"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7838770.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2889..3593)
                     /locus_tag="IVW29_001279"
     CDS             complement(2889..3593)
                     /locus_tag="IVW29_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838771.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3780..4025)
                     /locus_tag="IVW29_001280"
     CDS             complement(3780..4025)
                     /locus_tag="IVW29_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838772.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4042..4893)
                     /locus_tag="IVW29_001281"
     CDS             complement(4042..4893)
                     /locus_tag="IVW29_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7838773.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5059..6063
                     /locus_tag="IVW29_001282"
     CDS             5059..6063
                     /locus_tag="IVW29_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7838774.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6304..6660)
                     /locus_tag="IVW29_001283"
     CDS             complement(6304..6660)
                     /locus_tag="IVW29_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7838775.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7028..8215
                     /gene="tuf"
                     /locus_tag="IVW29_001284"
     CDS             7028..8215
                     /gene="tuf"
                     /locus_tag="IVW29_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7838776.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8403..10994)
                     /gene="mprF"
                     /locus_tag="IVW29_001285"
     CDS             complement(8403..10994)
                     /gene="mprF"
                     /locus_tag="IVW29_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7838777.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11020..11739)
                     /locus_tag="IVW29_001286"
     CDS             complement(11020..11739)
                     /locus_tag="IVW29_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7838778.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12059..12580
                     /locus_tag="IVW29_001287"
     CDS             12059..12580
                     /locus_tag="IVW29_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7838779.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12639..13499
                     /locus_tag="IVW29_001288"
     CDS             12639..13499
                     /locus_tag="IVW29_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7838780.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13606..15999
                     /locus_tag="IVW29_001289"
     CDS             13606..15999
                     /locus_tag="IVW29_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7838781.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16181..18022
                     /locus_tag="IVW29_001290"
     CDS             16181..18022
                     /locus_tag="IVW29_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7838782.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18046..19155
                     /gene="rpoD"
                     /locus_tag="IVW29_001291"
     CDS             18046..19155
                     /gene="rpoD"
                     /locus_tag="IVW29_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7838783.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19375..21474
                     /locus_tag="IVW29_001292"
     CDS             19375..21474
                     /locus_tag="IVW29_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7838784.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21670..22533
                     /locus_tag="IVW29_001293"
     CDS             21670..22533
                     /locus_tag="IVW29_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7838785.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT         7601 a         3967 c         4701 g         6304 t
ORIGIN      
        1 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
       61 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
      121 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
      181 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
      241 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
      301 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
      361 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
      421 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
      481 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
      541 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
      601 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
      661 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
      721 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
      781 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
      841 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
      901 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
      961 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     1021 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     1081 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     1141 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     1201 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     1261 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     1321 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     1381 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     1441 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     1501 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     1561 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     1621 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     1681 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     1741 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     1801 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     1861 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     1921 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     1981 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     2041 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     2101 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     2161 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     2221 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     2281 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     2341 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     2401 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     2461 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     2521 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     2581 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     2641 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     2701 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     2761 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     2821 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     2881 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     2941 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
     3001 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
     3061 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
     3121 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
     3181 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
     3241 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
     3301 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
     3361 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
     3421 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
     3481 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
     3541 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
     3601 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
     3661 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
     3721 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
     3781 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
     3841 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
     3901 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
     3961 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
     4021 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
     4081 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
     4141 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
     4201 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
     4261 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
     4321 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
     4381 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
     4441 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
     4501 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
     4561 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
     4621 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
     4681 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
     4741 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
     4801 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
     4861 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
     4921 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
     4981 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
     5041 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
     5101 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
     5161 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
     5221 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
     5281 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
     5341 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
     5401 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
     5461 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
     5521 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
     5581 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
     5641 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
     5701 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
     5761 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
     5821 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
     5881 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
     5941 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
     6001 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
     6061 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
     6121 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
     6181 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
     6241 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
     6301 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
     6361 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
     6421 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
     6481 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
     6541 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
     6601 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
     6661 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
     6721 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
     6781 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
     6841 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
     6901 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
     6961 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
     7021 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
     7081 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
     7141 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
     7201 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
     7261 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
     7321 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
     7381 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
     7441 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
     7501 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
     7561 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
     7621 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
     7681 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
     7741 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
     7801 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
     7861 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
     7921 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
     7981 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
     8041 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
     8101 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
     8161 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
     8221 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
     8281 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
     8341 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
     8401 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
     8461 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
     8521 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
     8581 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
     8641 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
     8701 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
     8761 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
     8821 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
     8881 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
     8941 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
     9001 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
     9061 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
     9121 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
     9181 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
     9241 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
     9301 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
     9361 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
     9421 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
     9481 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
     9541 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atccggcaga
     9601 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
     9661 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
     9721 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
     9781 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
     9841 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
     9901 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
     9961 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    10021 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    10081 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    10141 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    10201 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    10261 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    10321 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    10381 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    10441 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    10501 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    10561 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    10621 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    10681 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    10741 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    10801 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    10861 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    10921 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    10981 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    11041 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    11101 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    11161 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    11221 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    11281 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    11341 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    11401 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    11461 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    11521 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    11581 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    11641 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    11701 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    11761 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    11821 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    11881 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    11941 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    12001 gacggttatt ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg taaaacgagt
    12061 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    12121 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    12181 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    12241 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    12301 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    12361 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    12421 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    12481 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    12541 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    12601 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    12661 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    12721 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    12781 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    12841 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    12901 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    12961 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    13021 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tgcggcgctc
    13081 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    13141 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    13201 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    13261 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    13321 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    13381 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    13441 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    13501 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    13561 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    13621 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    13681 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    13741 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    13801 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    13861 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    13921 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    13981 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    14041 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    14101 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    14161 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    14221 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    14281 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    14341 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    14401 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    14461 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    14521 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    14581 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    14641 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    14701 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    14761 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    14821 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    14881 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    14941 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    15001 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    15061 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    15121 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    15181 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    15241 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    15301 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    15361 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    15421 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    15481 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    15541 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    15601 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    15661 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    15721 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    15781 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    15841 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    15901 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    15961 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    16021 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    16081 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    16141 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    16201 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    16261 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    16321 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    16381 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    16441 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    16501 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    16561 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    16621 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    16681 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    16741 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    16801 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    16861 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    16921 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    16981 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    17041 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    17101 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    17161 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    17221 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    17281 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    17341 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    17401 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    17461 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    17521 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    17581 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    17641 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    17701 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    17761 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    17821 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    17881 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    17941 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    18001 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    18061 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    18121 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    18181 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    18241 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    18301 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    18361 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    18421 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    18481 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    18541 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    18601 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    18661 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    18721 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    18781 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    18841 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    18901 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    18961 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    19021 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    19081 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    19141 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    19201 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    19261 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    19321 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    19381 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    19441 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    19501 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    19561 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    19621 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    19681 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    19741 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    19801 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    19861 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    19921 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    19981 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    20041 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    20101 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    20161 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    20221 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    20281 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    20341 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    20401 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    20461 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    20521 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    20581 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    20641 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    20701 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    20761 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    20821 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    20881 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    20941 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    21001 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    21061 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    21121 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    21181 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    21241 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    21301 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    21361 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    21421 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    21481 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    21541 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    21601 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    21661 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    21721 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    21781 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    21841 cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact
    21901 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    21961 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    22021 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    22081 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    22141 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    22201 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    22261 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    22321 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    22381 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    22441 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    22501 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    22561 gcttataata att
//
LOCUS       DACCVU010000038        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCVU010000038 DACCVU010000000
VERSION     DACCVU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.038"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..1371)
                     /locus_tag="IVW29_001294"
     CDS             complement(61..1371)
                     /locus_tag="IVW29_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7838786.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IVW29_001295"
     CDS             complement(1383..2801)
                     /locus_tag="IVW29_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7838787.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IVW29_001296"
     CDS             complement(3041..4609)
                     /locus_tag="IVW29_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7838788.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IVW29_001297"
     CDS             complement(4613..4894)
                     /locus_tag="IVW29_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7838789.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IVW29_001298"
     CDS             complement(4891..5814)
                     /locus_tag="IVW29_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7838790.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IVW29_001299"
     CDS             complement(5811..6689)
                     /locus_tag="IVW29_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7838791.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IVW29_001300"
     CDS             complement(6676..8013)
                     /locus_tag="IVW29_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7838792.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IVW29_001301"
     CDS             complement(8016..9119)
                     /locus_tag="IVW29_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7838793.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IVW29_001302"
     CDS             complement(9193..10701)
                     /locus_tag="IVW29_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7838794.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IVW29_001303"
     CDS             complement(10667..11359)
                     /locus_tag="IVW29_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7838795.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IVW29_001304"
     CDS             complement(11381..12355)
                     /locus_tag="IVW29_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838796.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IVW29_001305"
     CDS             12655..13527
                     /locus_tag="IVW29_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838797.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IVW29_001306"
     CDS             complement(14109..16307)
                     /locus_tag="IVW29_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838798.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IVW29_001307"
     CDS             16539..17531
                     /locus_tag="IVW29_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838799.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IVW29_001308"
     CDS             17591..19012
                     /locus_tag="IVW29_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7838800.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IVW29_001309"
     CDS             19005..20504
                     /locus_tag="IVW29_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7838801.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IVW29_001310"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IVW29_001310"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7838802.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4493 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 atttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACCVU010000039        21961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCVU010000039 DACCVU010000000
VERSION     DACCVU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.039"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            380..1282
                     /locus_tag="IVW29_001311"
     CDS             380..1282
                     /locus_tag="IVW29_001311"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7838803.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            1493..3268
                     /locus_tag="IVW29_001312"
     CDS             1493..3268
                     /locus_tag="IVW29_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7838804.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3334..4920)
                     /locus_tag="IVW29_001313"
     CDS             complement(3334..4920)
                     /locus_tag="IVW29_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7838805.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            5171..5395
                     /locus_tag="IVW29_001314"
     CDS             5171..5395
                     /locus_tag="IVW29_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7838806.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5537..7288
                     /locus_tag="IVW29_001315"
     CDS             5537..7288
                     /locus_tag="IVW29_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7838807.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            7288..9072
                     /locus_tag="IVW29_001316"
     CDS             7288..9072
                     /locus_tag="IVW29_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838808.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            9407..10259
                     /locus_tag="IVW29_001317"
                     /pseudo
     CDS             9407..10259
                     /locus_tag="IVW29_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            10356..12242
                     /locus_tag="IVW29_001318"
     CDS             10356..12242
                     /locus_tag="IVW29_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7838809.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(12283..13185)
                     /locus_tag="IVW29_001319"
     CDS             complement(12283..13185)
                     /locus_tag="IVW29_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7838810.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            13415..14818
                     /locus_tag="IVW29_001320"
     CDS             13415..14818
                     /locus_tag="IVW29_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7838811.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            14818..16251
                     /gene="ascB"
                     /locus_tag="IVW29_001321"
     CDS             14818..16251
                     /gene="ascB"
                     /locus_tag="IVW29_001321"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7838812.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16396..17322
                     /gene="trxB"
                     /locus_tag="IVW29_001322"
     CDS             16396..17322
                     /gene="trxB"
                     /locus_tag="IVW29_001322"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7838813.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17535..19262
                     /locus_tag="IVW29_001323"
     CDS             17535..19262
                     /locus_tag="IVW29_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7838814.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            19335..19628
                     /locus_tag="IVW29_001324"
     CDS             19335..19628
                     /locus_tag="IVW29_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7838815.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(19722..20582)
                     /locus_tag="IVW29_001325"
     CDS             complement(19722..20582)
                     /locus_tag="IVW29_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7838816.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            20674..21102
                     /locus_tag="IVW29_001326"
     CDS             20674..21102
                     /locus_tag="IVW29_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7838817.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21264..21440
                     /locus_tag="IVW29_001327"
     CDS             21264..21440
                     /locus_tag="IVW29_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7838818.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21467..21913
                     /locus_tag="IVW29_001328"
     CDS             21467..21913
                     /locus_tag="IVW29_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7838819.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7115 a         3853 c         4718 g         6275 t
ORIGIN      
        1 aaaacagtca atgattgaaa cactctaaaa ataatcttag gaacaaacca tttttctttt
       61 tttcttctat tctgtttttt tcgagtattt ttgcgtggca aaagggtcac ctcaactaat
      121 ggttttattt cgctaattat agcaaataat aggacaaaaa aacagacaag gctgtttttg
      181 acaggaattt ctttttcgaa tgagataaaa cgtgaggaaa tgatcttttt tatgaaagag
      241 ttcttaagct tttctttagg gaaagtttca gttactatgg agaacagggc aaagaaaaac
      301 aaaccatttt ctttgtacat catcttatca atatacagga attgttcgaa acaattgcct
      361 ttcaaggagg aatccatcca tgttattagg ctcacatgtc agcatgagcg gaaagaaaat
      421 gctgttaggc gcggcagaag aagcagccag ttacggatct tcgaccttta tgatctatac
      481 aggcgctcct caaaatacta gaagaaaagc tatcgaagag atgaatattg atgccggaaa
      541 agcatttatg gcagaaaacg gactaacaaa tatcgtcgtt catgcgcctt atatcattaa
      601 tttgggaaat acgatcaaac cagaaatgtt tccatttgct gtacaatttc tgagagaaga
      661 gattgtccgt gcggaagctt taggtgctac tcaaatcacc cttcacccag gcgctcatgt
      721 aggagcaggg gcagaagctg ggattgctag aattattgag gggttgaatg aagttttgac
      781 aaaagaacag acggcacaga tcgctttgga gacgatggca gggaaaggta cggaaatcgg
      841 tcgaacattt gaggaattag cgcaaatcat cgatggagta aaattgaatg aaaaattatc
      901 cgttactttt gatacatgtc atacgaatga tgccggatat aatataaaag aagatttttc
      961 aggtgtaatg gaagagtttg ataaggtcat cggattagat cggcttaaag tactgcatat
     1021 caatgattct aaaaatccac aaggttctca taaagatcgg catgccaata tcggttttgg
     1081 aacaattgga tttgacacat tgaacaaaat cgtccatgat ccaatgtttg acaacatttc
     1141 gaaaatatta gaaaccccat atgtcggtaa agataaaaag aaagcttacg caccatatgg
     1201 aaaagaaatt tccatgttca aagcgcaagc attcgatccc gaagtgttcc ctgagttatt
     1261 agcagaagaa gtcacacttt aagttttcta cctgacagca acaaaaaagg attgggcagt
     1321 ctgccttgtc cttttttgtt taagaaacaa aggagtgctt tttgcattcg ttttcgccat
     1381 gctccttttg ccccctcttt ttaaattcag acaaacaggg tatactgata gaaaagtaaa
     1441 aaacgaaaag tttcacaaac aagtagcaaa acgtgattga aaggacgaaa agatgctgaa
     1501 cattttaaac cccttgatcg accaactaaa aaagaaacat acatacttta ttcttggcat
     1561 agccgtatta tttacaatta ttcggttgta tttatatagt aaagcaatct atggaacaga
     1621 tatgtcggcg acctatgatg atcagctgct gattcactat gctcaaaata ttttggatgg
     1681 taaatggcta ggggagtata cgactacaac actttccaaa ggaatctcct acagcttatt
     1741 tttagtactt gcacataaac tgcatctttc ttatgcagtt ttgttcggca tgttcagtat
     1801 ttttgccagt atattggttg cactcgcgtt taaaccgatt gtgaagaaca agtatcttct
     1861 tttaagtatc tatttttatt ttttgttttc tccgattagt tttacacatg aatattccac
     1921 tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag atcattatcg cttgtttatt
     1981 agctatttat taccgcaaat acggttctag cagaaaattg ctgccatggt ttttagggct
     2041 ttctgtcgct tttcctttct tttggttcat tcgcgaagat tctatctggc ttcttccttt
     2101 tactgtagta gctttgggta tcagtttcgt ccagatcttg ctagggcaag acttttcttt
     2161 caaagaagta aagaagttaa aaaaacgact ctttcttctc tcgaaacggg aatggctgaa
     2221 actttcattg tttttacttc ctatagttgg cttttccatc gtctatcagt ggatcgaata
     2281 taaaaatgag acaacatacg gtattgcaac catcaatgac cgttccagtg gagaatttgg
     2341 acgtttgatg aaacaactga tccgaataga tgatgaaacg aacttgaata aagaaaacag
     2401 tgagatatgg gtctcacatg ctgcactaaa taaagcatta gccgcatctc ctacattcca
     2461 tactgtttct acccgtattg acgaattata tacgactcac gcgtggactc aaggtggaaa
     2521 agtaaaagaa ttacccggag atatcatctt ttgggcattg cgagatgtgg taagcgaatc
     2581 tggttattac caaaacaatg caaaagaaac caatgatttt tggaaaaaag tcaaccaaga
     2641 attagaagaa gcctatcaca agggaaccat caaaaagaaa aaagagttct atctgatggg
     2701 atcaggagat ggaaagacga tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg
     2761 atacgcttat gcatggcgat ataaagattt ccatcaagga ggaaattata gtttcggaac
     2821 gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt ccgctaatgg agaattggat
     2881 cgatagtaat gaaccgagaa cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa
     2941 tgtcatcata aaaatttacc aagttctggc tattcccgtg ctgattgctt caatagcagg
     3001 gatattactt atgatactag gaaccgtatt tgcaaaagct gatcgtgcct attatagttc
     3061 ctctttgatt gtggtcaccg gattaatact gacacaattg gtttttcttt tcggtgtctc
     3121 atggttttgt tcttatgcgc cagaaaaaaa ggactatttt ttaagtgtct acactggtgc
     3181 aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg ctagttttca gtaaattaaa
     3241 tctgaaaaaa gctttatctt ctaaataaaa agacccttgg tagctagatt tttaacctaa
     3301 ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg atttcttcta ccgtttcctg
     3361 caaaagctgt tcatattttt tgtttccttt atataaatca ccaatcgtcc atgccaacag
     3421 gagaaggaca agaacaatga ttacataagc gatgtgatca gccaatgcta gttgagcgga
     3481 agtggcttgc tcgccgaaga aactttcgat ctgtcctggt aatcctcgta agttcaagta
     3541 agttagacta atcacagaga accagccaag aagttttacg atgaatgaat ttttaaatcg
     3601 gctacccatc tccgcttggc tgttggtcat catcagtaaa ggaatcatcg aaaatggcaa
     3661 cgcaaaagcc aagaacacct gggattcatt cattaagtta ttcaaagcgg catgttcttc
     3721 gatcgttcct tttgtacttg taaacaaaac acaaatcaat acaggaatca cagaaagcaa
     3781 tcgtgtgacc agtcttctta gccaaatcgg gatacgcata tggatgaatc cttccatgat
     3841 gacttgaccg gtcaacgtac cagtaatagt agagttctgg ccagaagcaa gaagtgctac
     3901 agcaaataaa gtggacaaga taccagattt tgctacagca atcaaaatac cattgctcat
     3961 agctgatgga tcagataaag cttcatacag accaaaaaag gaaggatctt ggacagaacc
     4021 ggttttgaag acagcgacac ccatgatcaa tagcagtgca ttgactagaa aagccattga
     4081 caactggata ttagaatccc aagtagaaaa acgtacagct tgtgctacat cttcttcgtt
     4141 ttggtgatcg atcttccgag tttgtgaaac tgctgaatga agatataagt tatgcggcat
     4201 gactgttgca ccaataatcc ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg
     4261 tgagtctgag aatgtatgac tgttagggat aaagccagca aataaactgt gccaatctgg
     4321 gttagacaag ccaacttgat agacaaaaac gaataaaatg acgaaaatta agcagacaac
     4381 cagtgcttcg atcttacgaa acccgacttt tgtcagtagt aagagaacga aaacatctaa
     4441 tacagtaata aagacggcaa tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc
     4501 tgcaccaatg acttctgcga tgtctgttgc cataatggct aattcagtca agatccataa
     4561 aatgacaccc aatgtcttac ttgtccgtgc tcggattgct tgggccaaat ccatttgggt
     4621 cacgattcct aattttgctg ccatatactg taaaagcata gcaattagac tagacatcaa
     4681 aataactgac atcaataaat attggaaatt ttgtccacca gtgatcgaag tggaccagtt
     4741 acctggatcc atataaccaa ctgctactaa tgcacctggt ccagagtaag caagaagtgt
     4801 tttaaaaaaa ctttttccct ttggtacatc cactgttcca tttacttcag ctaaagataa
     4861 accatttgta tgttcaataa gtcgatgctt atgaaattgt ttgttggatt gattattcaa
     4921 gaaaaaacca cctttcctat ttatctacga ttacattcta cacctcagaa agaaaaaatt
     4981 aaagaaaaag attcggtatg cctaattttt tttcgtggtg tgtcttgctg gttaatgaaa
     5041 gaaattttgt tcttctctaa aaaaaccact attttttgca tgaaactaaa gaatacgcct
     5101 ttcttttttc aataaacttt agtaaaataa atcattgaga gcaattcgga aatagaaagg
     5161 agagatttat atggtatcaa caggtattgt cgtacttatt gctattattg ctttgctaat
     5221 cggcgcagta ggtggctttt tcctagcgcg taaatatatg aaagactatt tagaaaaaaa
     5281 tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc
     5341 tgagaagaaa attcgccaga tgatgcagca aatgaagaac caagggaaaa aataggttct
     5401 gacccaaaga ctttttgcgg gctgattttt tcagtccgtt ttttatttcg taatttgtta
     5461 gactattcag actattcagc aaaaaagaca agcacagaca atcctatgct cttgataaag
     5521 acaaaggagg agtaaaatgt cgatttttag aaaattaggt tggttttttc gtcaggaaaa
     5581 gaaacattat ttaattggga ttttctccct attcgcagtg gccttggtac agttagttcc
     5641 tccaaaagtg attgggatca tcattgatga gatcgccgat aagaatttat caataaagat
     5701 catttcctgg tggatcttat tattgatcct tgcagctttc ttacaatatc tttttcgtta
     5761 tatctggcgg acgaatattt ggggaagtgc agctcggttg gagagagaac ttcggcagca
     5821 gctattcaaa cattttacag aaatggacaa tctcttttac caaaaatatc ggactggaga
     5881 tttgatggct catgcaacga atgatttgaa tgcaatccag aatgtagcag gagctggaat
     5941 cttgaccttt gcagattcag tgattacggg cggaacaacg atcattgcga tgattttgtt
     6001 tattgattgg cgcttaacac tgattgcgct gattcctctg cctttgctag ccgttacttc
     6061 acgagtgctg gggtcaaaac tccacgatgc ttttcgtgat tcgcaagaag ctttttctag
     6121 tatcaatgat aagacacaag aaagtatcac tgggatcaaa gtcattaaaa catttggaca
     6181 agaaaaagaa gatctcgcgg attttgaaga aaagattgaa gatgcaatta ttaaaaacaa
     6241 acgagtcaat tttttagact ccttatttga tccattcatt accttgatta tcggtctgtc
     6301 ttatgttgca acaattattt taggtggaaa gtatgtcttg gaaggcacga tctcaatcgg
     6361 gcagatggtc tcatttatta gctatatcgg tatgcttgtt tggccgatgt ttgcgattgg
     6421 tcgattgttc aacgtattgg aaagaggaaa tgcttcttat gatcgcgtca atgaactttt
     6481 gcatgaaaag acgcatatcg tggaacaact gggtgcagtg cagaccccgg caaaaggcga
     6541 gctgaatgta gctatttcct cttttaacta tccgaaagaa gaatcaccag cattagaaca
     6601 aatcaaattt gcgatccaag aaggagaaac attaggtatt gttgggaaaa ctggtgcggg
     6661 aaaaacgaca atcatcaaat tactgatgcg tgaatacgat gattatcaag ggaaaatcca
     6721 atttggcgga cgttcgatca aagaatattc attgaatgct ttattaggat caatcggtta
     6781 tgtgcctcag gatcacttcc tgttttcgat gaccattcga gataatatcc gtttttctaa
     6841 tccaaaactt tcagaagaag aagtagagca tgcggcttct ttagcattta tcaacaatga
     6901 aatcaaggaa ttgccaaaag gttatgatac cttagttgga gaacgtggag tctctttatc
     6961 tggcggacag aaacagagga tttcgattgc tcgagcattg atcaaaaatc ctgaattgct
     7021 gatcttagat gatgcacttt ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa
     7081 tttaaaagaa ataagaaaag agaaaacaac catcattgca gctcaccgtt taagcagcgt
     7141 gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa atcattgaac gaggaaccca
     7201 ccacgagctg cttgcattag gtggctggta tgaacgaatg tgggaaaaac agcaattaga
     7261 agcgaagata gaaggaggcg aggcctgatg gatgaaaatt atcaatcgga atggacaaaa
     7321 tcgattccac ttaaagaaca gaagaagatc atcaaacgac tgatgaagtt cgccaaacct
     7381 tttagaagaa cgttcatcgt tgctatctta tttgcgtttg ccctgtctgt gatcaatgtc
     7441 ctattgccaa ggatcatcca aacttttatg gatgatcatc tggctaaaca gtcagctacg
     7501 acacaagtta ttttgttttt tgctggtgtg tacttgtttg gagtcatagt caaaagtatc
     7561 atttggtttt tccaatggta tttgtattcc atggcttcat tgaagaccta tcagtatgtt
     7621 cgggtaagat tatttgaaaa gctgcataca ctaggtatgc gttattttga ccagacaccg
     7681 gcaggatcga ccgtttctcg agtgacgaat gacacagaaa cgctatttga attttggtat
     7741 gtctttttga tggtattgac tgggatcttt gcagtcgttt cttctttcgt tgccatgttt
     7801 caaatcaatg gagaaattgc cttgtggact ttggtgttct tgccgatttt aggaatcgtc
     7861 atctggtatt accagaaatt cagttctcgt atttaccgaa atatgcggga aaaattaagt
     7921 cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc gtattatcca gcagtttcgc
     7981 caagaagctc gtttagctaa agaatttgaa caagtgaaca acgagtattt agacacgcgc
     8041 tatgcgatga tcaaaacgaa ttcgattctg ttaagtccaa tcatcaattt tctctatgca
     8101 ttggcgattg cgttggcgtt gactttgttt gggatcgatg cgttgcattc tcccgtagaa
     8161 gttggactga tctatgcatt tacgacctat gtccaggaat tttttaatcc aatgtctcaa
     8221 atgatggact ttttgagcac ctttacagat gggatggttg ccggtagtcg tattttgaaa
     8281 atcatggata cagaagaata tgcaccgcaa caaaatcctg gagcagatca agcgattact
     8341 tcaggtaaga tcgagtttcg caatgtaagc ttttcttatg atggagagca caatgtattg
     8401 aacaatatct cttttgtcgc taatccgggt gagaccgttg cattagtggg gcacaccgga
     8461 agcggaaaaa gctcgatcat caacgtattg atgcggtttt acgaatttta tgaaggccaa
     8521 atcttgattg atgatcatga cattcgagaa tatccaatgg aagagttacg taagaaaatg
     8581 ggattggtct tgcaagatgc cttcttgttt tatggagata ttgcaggaaa tatccggatg
     8641 atgaatccag ctatcacaga cgaacagatt cgccatgcag ctgagtttgt tcaagcaaac
     8701 aagttcatcg atgaattacc aaaaggatat cactcaaaag tgatcgaaca cggtgccagc
     8761 tattctagtg gacaacgaca gctgatcact tttgcgcgaa caatcgtgac agatcctaaa
     8821 atactagtat tggatgaagc aacagcaaat atcgatactg aaaccgaagg attgatccaa
     8881 gaaggattgg caaaaatgcg acaaggacgg acaacgatag cgattgccca tcggctgtcc
     8941 acgattcggg atgcagaatt gattttagtt ttagacaaag gtcatatagt agagcgtggg
     9001 aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc
     9061 agtggtgtgt aaaaagaggt tgtgacagta gcatagtagc attttgccct aagcgctaga
     9121 gtgaaaataa aacagagctt cgcagattct tcatctgtga gcgttagtgg cgtagtcacc
     9181 cgatttaaaa aagacgacga cagctagtca aaagcggcta ataaccacta agaaagcgtt
     9241 gacaaaagcg tttgctttcg ataatataaa gaaaacgata caacttggat agtgattgcc
     9301 tgatgcaagc taaacctgat gagtctccca tggttccatg gacggctttt tgggtttttc
     9361 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
     9421 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
     9481 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
     9541 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
     9601 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
     9661 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
     9721 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
     9781 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
     9841 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
     9901 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
     9961 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
    10021 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
    10081 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
    10141 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
    10201 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
    10261 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
    10321 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
    10381 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
    10441 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
    10501 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
    10561 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
    10621 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
    10681 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
    10741 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
    10801 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
    10861 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
    10921 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
    10981 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
    11041 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
    11101 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
    11161 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
    11221 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
    11281 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
    11341 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
    11401 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
    11461 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
    11521 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
    11581 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
    11641 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
    11701 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
    11761 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
    11821 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
    11881 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
    11941 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
    12001 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
    12061 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
    12121 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
    12181 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
    12241 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
    12301 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
    12361 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
    12421 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
    12481 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
    12541 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
    12601 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
    12661 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
    12721 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
    12781 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
    12841 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
    12901 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
    12961 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
    13021 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
    13081 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
    13141 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
    13201 ttgttttttt acgtaaatca taactttttt gaaaatgatt tgtaaacgga tttatgttat
    13261 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
    13321 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
    13381 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
    13441 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
    13501 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    13561 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
    13621 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    13681 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
    13741 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
    13801 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
    13861 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    13921 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
    13981 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
    14041 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
    14101 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
    14161 catgatccct tgtggctacc accgctatta ctatcgtgaa gaagaaatgc tggaacatgc
    14221 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    14281 gctgtttgaa ttatacaaag atccagaatt ggatcataaa cctgaacaat tgcaaaaacg
    14341 aggtggtgct tattattcag atgctgcctg tgaaacgatt gcttcgatct atggaaataa
    14401 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgatc tgccggcaga
    14461 ttgtgtagta gaagtcactg cctatgttgg cgcacaaggc gcacgaaatg tagcgtttgg
    14521 ttcccttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    14581 gatcgaagca gccgtgactg gtgattatgg aacagctttg caagcgttta cgatcaatcc
    14641 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacatca
    14701 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
    14761 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
    14821 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
    14881 tatgatgaag gcggacgtgg gttagcgaac gtagatgttg tgccaatcgg agaagaccgt
    14941 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
    15001 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
    15061 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
    15121 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
    15181 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
    15241 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
    15301 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
    15361 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
    15421 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    15481 atcgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    15541 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaagtcaga tcgagaaaat
    15601 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    15661 gaacgaaatg gcattcagat caaaatggaa cctggggatg ccgcattgct gaaagaacat
    15721 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
    15781 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
    15841 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    15901 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
    15961 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccag
    16021 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
    16081 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
    16141 gtcgatcgtg acaacgaagg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
    16201 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt
    16261 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    16321 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    16381 aaggagaatc tacatatgta tgatgtaatc gttattggcg cagggcctgc tgggatgact
    16441 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgctcct
    16501 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
    16561 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
    16621 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
    16681 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
    16741 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
    16801 gcatttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
    16861 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
    16921 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
    16981 gatacagtcg ttgacgaaat cgttggaaat gaaatggtag tgacaggtgt gaaagcacgt
    17041 aatgtcaaaa ctggcaatga gtttgatttg gcagcagacg gagtattcat ctatgtagga
    17101 ttggatccat taactgcacc atttagagaa gctgggttga cgaacgaagc cggctggatt
    17161 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
    17221 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggattgc tggacaacaa
    17281 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtgaaag
    17341 aacggtttca attgttcttt tactcttttt tttgcgtgaa atctactgaa tccgatttat
    17401 gcgaatactt gtaaccgtgc ttgataaaag aatcttttct tgatttaagc gttttttggt
    17461 cctcatactt tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa
    17521 gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt
    17581 tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag
    17641 atgcttttta tgcaccgttg gaattcggta ctgcaggcat gcggggaatc ttaggacctg
    17701 ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggactt gcacgtttca
    17761 tggatacaca aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc
    17821 atcaatcacc agaatttgcc atggaagcag ccaaaacatt agcacatcac aatatccctt
    17881 cttatgtatt cgaaagttta cgtccaacac cagagctttc ttttgcagtc cgtcatttga
    17941 agacgtttgc gggaatcatg gtaactgcat cccacaatcc agcagcttac aacggatata
    18001 aagtctacgg agaagatggg ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat
    18061 atgtccgtga agtcagcaat ccgttaaaag ttgaagtatt atctgacgac gaagctgaac
    18121 atagcggatt gatcacgatc atcggagaag aagtggatgc cgcttatcta gaagaaatca
    18181 aagcagtaac gattgatcat gaattggtgg aaacaatggg taaagatttg aagcttgtgt
    18241 acacaccact tcacggaaca ggaaaaatgt tgggcgaacg tgcgttgaaa caagctggat
    18301 tcgaacaatt cgtactagtt ccagaacaag ctgtcgcaga cccggatttc actacagtca
    18361 aatcaccgaa tccagaagaa cactctgctt tcgagtatgc gatgcgactt ggcgagaaag
    18421 aaggcgctga cttactgatt gcaacggatc ctgatgcaga ccgtttaggc gcagccgttc
    18481 gtttgccaaa tggctcctat gaagtattga caggaaacca aatcggagcc ctgtttgctc
    18541 aatatatttt ggaagcacac caatcgcgag gcactttacc tgaaaatgca gcggtattga
    18601 aatcaatcgt ttctagcgaa ttgcctacag caattgcgga acactatggg gctaccatgt
    18661 tcaatgtatt gacaggattc aaattcattg ctgaaaaaat tcaacaattt gaagaagatc
    18721 attcccatac ttttatgttc ggattcgaag aaagttacgg ttatttagtt aaacctttcg
    18781 ttcgcgacaa agacgcgatt caagcattgc tcttattagc agaagtagca gcttactata
    18841 aaaaacaagg caaaacattg tatgacggct tgcaaacgat ttttgaaaca tatggctact
    18901 acgcagaaaa aacgatttct gtgacaatga gcggacaaga aggctctgca aaaatctctg
    18961 ctttgatgga aaaattccgt gaagaagcac cgacagaatt tgccggagca aaagtcatcc
    19021 aaacagaaga cttcaaagag ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa
    19081 cgacaccgcc atctgacgta ttgaagtata cgctggaaga aaatggctgg atcgctatcc
    19141 gtccttcagg aactgagccg aaaatcaaat tttatatcgg tgtaaaagga aactcttcag
    19201 aagacgcagc tgaaaaagta gccgcttatg aagcgattat caaaaaaatg acagaagaat
    19261 aaaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt
    19321 tggaggtgaa tggattgcca gacacgcaaa aaacaaaagt agagaaagtc agccaactat
    19381 tcaaaatgtt gagtgacccc actcgtctca aaattctgct gtatctgaaa gacggggaac
    19441 aaaatgtgac agcaatcacc caagcagtag aaatggaaca atctgctgta tcgcatcaac
    19501 tgcgtttatt aagagaaaac catgtcgtca agtcccatcg tgagggaaaa gcgattttgt
    19561 atagtcttga tgattctcac gtactggata ttttgaacca gacgctcaaa catgtggaac
    19621 atcaataacg ataggtgcaa atagagacaa actggactgt atcaacgaat cttcttcgct
    19681 gacacagtcc agttttttta agaaaaagac aagaaaaact gttagtctgt ttcttcggtg
    19741 gtttcagttc cataataact atgatcttct aggtctaaag cattcaaaat aagtgaagct
    19801 gtttcttcga tagataaaga agcgacattg attactacgc agcccaattt ttcataaaga
    19861 tcattcgcaa aatcgagttc agctcgtatt ttttcgatat cggaataagc agtatcagga
    19921 tttaatccgt aagagcgcat ccgttcttta cggatattgt ttaaaatatc tgggttattc
    19981 gtcagtccca cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt
    20041 gggatcaaag gtagattggc aacctttaaa tttttgttag ctaaaaacaa gctcaaaggc
    20101 gttttggaag ttctggatac gcctagcaaa acgacatctg cttctagaaa cccacgagga
    20161 tctttcccgt catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc
    20221 tcattcaata aatgaagggc gccaggttgc cgaatgggct tgataccagt taagcgctcg
    20281 atttctgata tcggcggagt catcacatca aaactaaata attgatgttc ttcacaaaaa
    20341 gaacgcgtca tctcgatcaa ttcttgattg atcaatgtgt gtaggacaag ggctttttct
    20401 tttagtgcat cttgcagtgc ctgcatcaat gttttctcat ctgtcacaaa tgttctgcgg
    20461 aataaattga actcaacggt aggatattgg gccatagtgg cttgcgctaa ttttgtcgca
    20521 gtttctccag cagagtcaga gatgacaaaa aaagtaatga tttctgtaga atcggaagac
    20581 atggataaaa cctcacttgc atttttttta tgtttattat accatactct agaatgtgta
    20641 cttcgtaatc attttgattt ggaggaataa gtcatgaaaa aacagacatt gattgaacaa
    20701 tcactagaaa cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta
    20761 acttatttag tgaagcgcaa tcgctacgta tccgctaaag aagtttttga ctatatgaat
    20821 caggaattca aaggagtaag ttacgatacg atttatcgaa atctccatga ttttgcagaa
    20881 atcgatttac tggaagaaac cgatttaaat ggagagatga agtttcgttt tcattgctgt
    20941 caaggggaaa tcggccatca ccatcacttt atctgtacgg tgtgcggaac aacgaaagaa
    21001 atccatatgt gtccaatgaa ttatttcgaa gaacagctcg gtggatgtac gatcgaaggg
    21061 catagattcg agattttcgg aagatgtgaa aattgtcaat aaggatttaa ataaaataac
    21121 atttgataat tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa
    21181 tatttacagg acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt
    21241 aagctcggag ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg
    21301 acgatgctct tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc
    21361 gtaaacgtga attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta
    21421 gaaaacgcaa aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag
    21481 tagattaaac gatgacatga aaacagcgat gaaagcgaaa gataaggaat ctttacaagt
    21541 catccgaatg atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac
    21601 agaagaagaa gaattaacgg tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca
    21661 tgagtttgaa gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtgat
    21721 agtcgaaaaa tacatgccgg aacaactttc cgacgaagaa attcgacaac ttgttcaaga
    21781 agcaatcaca caaactggtg cttcatcaat gaaagaattt ggcaaagtca tgggtgcgat
    21841 catgcctaaa gtcaaaggaa aagcagatgg aaaccaagta aatgccattg tgaaagaact
    21901 attacaatct taattttatc atttaaaggc tcttcgtcaa tgagtgttgg tgaagaaatt
    21961 c
//
LOCUS       DACCVU010000040        21941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCVU010000040 DACCVU010000000
VERSION     DACCVU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.040"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(39..482)
                     /locus_tag="IVW29_001329"
     CDS             complement(39..482)
                     /locus_tag="IVW29_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838820.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(498..1658)
                     /locus_tag="IVW29_001330"
     CDS             complement(498..1658)
                     /locus_tag="IVW29_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7838821.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2165..3865
                     /locus_tag="IVW29_001331"
     CDS             2165..3865
                     /locus_tag="IVW29_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838822.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3967..4800)
                     /locus_tag="IVW29_001332"
     CDS             complement(3967..4800)
                     /locus_tag="IVW29_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7838823.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5104..7269)
                     /locus_tag="IVW29_001333"
     CDS             complement(5104..7269)
                     /locus_tag="IVW29_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7838824.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7529..9823
                     /locus_tag="IVW29_001334"
     CDS             7529..9823
                     /locus_tag="IVW29_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7838825.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9816..10481
                     /gene="pgmB"
                     /locus_tag="IVW29_001335"
     CDS             9816..10481
                     /gene="pgmB"
                     /locus_tag="IVW29_001335"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7838826.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10474..11481
                     /locus_tag="IVW29_001336"
     CDS             10474..11481
                     /locus_tag="IVW29_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7838827.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11503..12522
                     /locus_tag="IVW29_001337"
     CDS             11503..12522
                     /locus_tag="IVW29_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7838828.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12803..14170)
                     /gene="nhaC"
                     /locus_tag="IVW29_001338"
     CDS             complement(12803..14170)
                     /gene="nhaC"
                     /locus_tag="IVW29_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7838829.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14279..15700)
                     /locus_tag="IVW29_001339"
     CDS             complement(14279..15700)
                     /locus_tag="IVW29_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7838830.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15943..17820)
                     /gene="tdc"
                     /locus_tag="IVW29_001340"
     CDS             complement(15943..17820)
                     /gene="tdc"
                     /locus_tag="IVW29_001340"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7838831.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18111..19367)
                     /locus_tag="IVW29_001341"
     CDS             complement(18111..19367)
                     /locus_tag="IVW29_001341"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7838832.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            19947..21248
                     /locus_tag="IVW29_001342"
     CDS             19947..21248
                     /locus_tag="IVW29_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7838833.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21317..21916)
                     /locus_tag="IVW29_001343"
     CDS             complement(21317..21916)
                     /locus_tag="IVW29_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838834.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6612 a         4588 c         3922 g         6819 t
ORIGIN      
        1 aaaattaggc tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc
       61 catttcatca caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta
      121 aaaccttgtg aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc
      181 agtgttttat tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg
      241 tattcccttt tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata
      301 atttcttgat ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat
      361 tctacttgat tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc
      421 agccaaatcg atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc
      481 attttttctc tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct
      541 tttacaagtt ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca
      601 aaagaaacag caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat
      661 tgatccagct tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta
      721 gtaagtgttt ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac
      781 gaatcactaa taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt
      841 agtcgtccga taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac
      901 cattgccgca tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta
      961 taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat
     1021 gtaagcaagg taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc
     1081 agtaccactt gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata
     1141 aacgtgtctc cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg
     1201 ctgtgagcag agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc
     1261 aagacgcctg ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt
     1321 aattggatct taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt
     1381 gtactttcat ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca
     1441 gttaatcgtt ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc
     1501 cgtttttgta cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa
     1561 aatggatata aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac
     1621 aaaagtcgac gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt
     1681 ttttgttctt tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag
     1741 gttgtgaaaa agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat
     1801 attttttgtt gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
     1861 gaggttgtga aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc
     1921 tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc
     1981 gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa
     2041 ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc
     2101 ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg
     2161 atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc
     2221 atttttagca atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa
     2281 actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca
     2341 aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag
     2401 gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct
     2461 tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg
     2521 attttctatg ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt
     2581 agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac
     2641 tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca
     2701 taaaaagttc cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt
     2761 actgtcttcg atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa
     2821 ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt
     2881 tctgacagca aatatttggg caggattagc tattgtttac agtgggaata agattcttcc
     2941 tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct
     3001 ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt
     3061 tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt
     3121 tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag
     3181 tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct
     3241 tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg
     3301 cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga
     3361 aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa
     3421 aacattcttc agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga
     3481 ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat
     3541 actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg
     3601 gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct
     3661 acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt
     3721 gaagcagcaa aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta
     3781 tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca
     3841 tagatacaag cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct
     3901 ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta
     3961 tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg
     4021 actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa
     4081 aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac
     4141 agtagctgct tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact
     4201 ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa
     4261 aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc
     4321 acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc
     4381 tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg
     4441 ggagacggta aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc
     4501 atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc
     4561 ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt
     4621 taccggacag aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt
     4681 gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg
     4741 caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat
     4801 tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac
     4861 ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag
     4921 ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc
     4981 actaaacagt attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga
     5041 ggagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc
     5101 tccttacatt ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat
     5161 cactacttct tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc
     5221 gttttggatc acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc
     5281 ttctttgaca gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg
     5341 gatcaaaact tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat
     5401 acttgtcact tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa
     5461 gccgtcaccc atcatttttt gtgagaatac cggatttttt acttcagata aaggaatcac
     5521 ttctccagaa gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc
     5581 ttgtccattt cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag
     5641 atcttcgata tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt
     5701 gtctttgacg atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt
     5761 ttctcgatca tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt
     5821 ttcttttcct cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc
     5881 gctgttagat acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt
     5941 agcaaagttg aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc
     6001 gatcacacac aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg
     6061 tgtccgagtc agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc
     6121 catcgcaaat gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa
     6181 cataaacatg aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga
     6241 aaaatacatt gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa
     6301 agccattccc atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg
     6361 cgtccagtta tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac
     6421 taaatcagtg gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg
     6481 tgcgcctgat aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag
     6541 catatgatgc aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc
     6601 tagaatcggt gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc
     6661 ctgtacgttt ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac
     6721 aacaaacgga acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata
     6781 attatagtac ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac
     6841 tcccatattc aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt
     6901 cgtaccgaac agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc
     6961 aaagatagcc ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc
     7021 gcctgctctt tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt
     7081 accaataatt gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca
     7141 atcaggattg atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat
     7201 aactgctacc acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat
     7261 ctttttcatt ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc
     7321 gttttctttt ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc
     7381 tataatacgt gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt
     7441 tctatgaaat attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg
     7501 tataaaacag ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga
     7561 tccatggaaa atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt
     7621 aacaagtatt ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg
     7681 ggaccaccat caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg
     7741 ctggtggaaa aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat
     7801 cgcgatagat atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga
     7861 cttttactgg gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac
     7921 ttctactaat aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc
     7981 agcttatatc tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc
     8041 aaaattggat ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga
     8101 gattgatcgt ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa
     8161 ctttgggatt gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc
     8221 agtcatccct gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt
     8281 atctcaaggt gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat
     8341 tccagaagcg cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta
     8401 tatccaagca aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt
     8461 ggtcatcgaa ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt
     8521 ctctacttac tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga
     8581 aaaatacggc ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc
     8641 tttagccaaa cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca
     8701 agcaatccac aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt
     8761 cactggtgtc gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg
     8821 cgcgattgcc tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa
     8881 aaatgaagga ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt
     8941 ttcaaaacga gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa
     9001 caatatcaat aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg
     9061 agaaaattat ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc
     9121 caaatggaaa gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt
     9181 tgtccagcat gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag
     9241 cgatcttcct ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa
     9301 gcaggcagat gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa
     9361 gcaaaaaaac tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag
     9421 tatccattcg attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca
     9481 gcgaacagct cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat
     9541 cacttccatg actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc
     9601 ccatgatcag ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca
     9661 tatcaattat cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga
     9721 gttattagaa ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga
     9781 tcgtctagaa ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt
     9841 tagacggtgt cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc
     9901 aggaaatcgg cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag
     9961 aagactcact ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag
    10021 aatttgcaga attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg
    10081 aaccaaaaga tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa
    10141 tcaagattgc tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc
    10201 ttacacctta ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc
    10261 cagatatctt cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga
    10321 tcgaagacgc gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg
    10381 tgggacgtgc tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta
    10441 cgatcgatta cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta
    10501 ccagttccag agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt
    10561 cttctcaatt acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca
    10621 gaaaatgttt tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt
    10681 ggagcaactg tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga
    10741 ttggagaaaa actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa
    10801 ttttgggaag tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa
    10861 gatgaatttt ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa
    10921 aacgaattag agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc
    10981 aaccatgcct acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta
    11041 acggtctttt tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg
    11101 acaaaaaaag aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt
    11161 gcatgttatc cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa
    11221 acttccttac aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat
    11281 aaaagtatgg tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa
    11341 cagatgcatt caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat
    11401 ttccccaaat ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt
    11461 tatcggttct ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa
    11521 gatgtggcga aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat
    11581 cctagtattt ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat
    11641 gttcctaaca tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc
    11701 ttaccccctc ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa
    11761 gcaatcaacg aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat
    11821 tttgaattgc tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt
    11881 attctagtgt attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc
    11941 cctttcactt tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat
    12001 gataaccagc ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat
    12061 atcctgtttg tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat
    12121 caaaaagcaa tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg
    12181 gaggattatc tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc
    12241 attgacgata tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt
    12301 ccagagacac tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct
    12361 taccttacaa gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg
    12421 cgcgaattga tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg
    12481 atcaaaagag agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat
    12541 acgctgacca tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac
    12601 attgtttatc tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    12661 taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca
    12721 aaaatttaaa aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt
    12781 cccagcctct tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc
    12841 ctaatattgt aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg
    12901 cgctaacttt catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat
    12961 tgacggtcgc acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt
    13021 catcaaaaga acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa
    13081 cgatcagatt gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt
    13141 ccatcttttc ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc
    13201 cacctaatgc caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc
    13261 ctcgagaaag cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca
    13321 agatgtctgc tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag
    13381 cagctgtcga tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa
    13441 ataaaataac tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt
    13501 ggatcatttc attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat
    13561 aaaaaatcaa cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt
    13621 cgaatagatt aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc
    13681 agttattccc taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc
    13741 caagtaaatg accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac
    13801 tgccgacagt cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca
    13861 cagaaacaag tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg
    13921 aagcaactaa gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc
    13981 ctttgatgat cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca
    14041 acaaacacat gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca
    14101 gtactcctag gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat
    14161 ttttattcat cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc
    14221 acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt
    14281 atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga
    14341 tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca
    14401 atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc
    14461 tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg
    14521 gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa
    14581 taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat
    14641 aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc
    14701 cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt
    14761 ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa
    14821 ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa
    14881 tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta
    14941 caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt
    15001 ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc
    15061 caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag
    15121 ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga
    15181 aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata
    15241 caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc
    15301 agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac
    15361 caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca
    15421 tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta
    15481 cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg
    15541 atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga
    15601 ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa
    15661 aggtcccaag acccatttta cttacttcag atttactcac cgttgacact ctccttgatt
    15721 ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg
    15781 aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa
    15841 aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga
    15901 aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc
    15961 caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc
    16021 agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa
    16081 tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa
    16141 ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc
    16201 attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt
    16261 attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa
    16321 atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc
    16381 attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata
    16441 tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc
    16501 tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc
    16561 aaatacatat gtcgcaaagt aagagataac gtttctcatg cggatatctt tgatgacgat
    16621 accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc
    16681 ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt
    16741 aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc
    16801 ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa
    16861 gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat
    16921 tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc
    16981 tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac
    17041 tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga
    17101 atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg
    17161 tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat
    17221 ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt
    17281 cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa
    17341 gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt
    17401 tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc
    17461 aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt
    17521 ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg
    17581 catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact
    17641 agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg
    17701 ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc
    17761 agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat
    17821 agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt
    17881 ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat
    17941 ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact
    18001 ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt
    18061 ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt
    18121 actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat
    18181 tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata
    18241 gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca
    18301 agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat
    18361 ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt
    18421 attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc
    18481 aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg
    18541 atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct
    18601 tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg
    18661 gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt
    18721 aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg
    18781 atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga
    18841 tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact
    18901 tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg
    18961 tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat
    19021 gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta
    19081 tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg
    19141 atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt
    19201 ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg
    19261 ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt
    19321 tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc
    19381 gtgtagtttt tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg
    19441 ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt
    19501 accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg
    19561 tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc
    19621 agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct
    19681 tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg
    19741 atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt
    19801 attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga
    19861 agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa
    19921 aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca
    19981 cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta
    20041 caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag
    20101 atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc
    20161 aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc
    20221 ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct
    20281 ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg
    20341 cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac
    20401 tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag
    20461 gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac
    20521 aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg
    20581 tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg
    20641 actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc
    20701 gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat
    20761 caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag
    20821 tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga
    20881 atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag
    20941 ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg
    21001 actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg
    21061 atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct
    21121 atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa
    21181 atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat
    21241 ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt
    21301 tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc
    21361 attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca
    21421 ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt
    21481 tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca
    21541 tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa
    21601 attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt
    21661 cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa
    21721 atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg
    21781 ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt
    21841 ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct
    21901 tttcaaaacc gttcatttgt agagcctccc gtaattttaa a
//
LOCUS       DACCVU010000041        21809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCVU010000041 DACCVU010000000
VERSION     DACCVU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.041"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..671
                     /locus_tag="IVW29_001344"
     CDS             <1..671
                     /locus_tag="IVW29_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7838835.1"
                     /translation="QYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKE
                     IANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKI
                     CCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISI
                     SNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQV
                     ILLF"
     gene            686..1438
                     /locus_tag="IVW29_001345"
     CDS             686..1438
                     /locus_tag="IVW29_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838836.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2141..2356)
                     /locus_tag="IVW29_001346"
     CDS             complement(2141..2356)
                     /locus_tag="IVW29_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7838837.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2375..2611)
                     /locus_tag="IVW29_001347"
     CDS             complement(2375..2611)
                     /locus_tag="IVW29_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7838838.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2869..5022
                     /locus_tag="IVW29_001348"
     CDS             2869..5022
                     /locus_tag="IVW29_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP7838839.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4991..6406
                     /locus_tag="IVW29_001349"
     CDS             4991..6406
                     /locus_tag="IVW29_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP7838840.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6439..7473
                     /gene="sppA"
                     /locus_tag="IVW29_001350"
     CDS             6439..7473
                     /gene="sppA"
                     /locus_tag="IVW29_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7838841.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7475..7996
                     /locus_tag="IVW29_001351"
     CDS             7475..7996
                     /locus_tag="IVW29_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7838842.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7993..8379
                     /locus_tag="IVW29_001352"
     CDS             7993..8379
                     /locus_tag="IVW29_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7838843.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8875..8947)
                     /locus_tag="IVW29_001353"
     tRNA            complement(8875..8947)
                     /locus_tag="IVW29_001353"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8912..8914),aa:Lys,seq:ctt)
     gene            9328..9897
                     /locus_tag="IVW29_001354"
     CDS             9328..9897
                     /locus_tag="IVW29_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7838844.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9884..10744
                     /locus_tag="IVW29_001355"
     CDS             9884..10744
                     /locus_tag="IVW29_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7838845.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10934..11638
                     /locus_tag="IVW29_001356"
     CDS             10934..11638
                     /locus_tag="IVW29_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7838846.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            11643..13478
                     /gene="walK"
                     /locus_tag="IVW29_001357"
     CDS             11643..13478
                     /gene="walK"
                     /locus_tag="IVW29_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7838847.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13475..14791
                     /locus_tag="IVW29_001358"
     CDS             13475..14791
                     /locus_tag="IVW29_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838848.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14792..15661
                     /locus_tag="IVW29_001359"
     CDS             14792..15661
                     /locus_tag="IVW29_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838849.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            15716..16525
                     /locus_tag="IVW29_001360"
     CDS             15716..16525
                     /locus_tag="IVW29_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7838850.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHINGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(16628..17257)
                     /locus_tag="IVW29_001361"
     CDS             complement(16628..17257)
                     /locus_tag="IVW29_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP7838851.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17427..18719
                     /locus_tag="IVW29_001362"
     CDS             17427..18719
                     /locus_tag="IVW29_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7838852.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18795..19457)
                     /locus_tag="IVW29_001363"
     CDS             complement(18795..19457)
                     /locus_tag="IVW29_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7838853.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            19682..19966
                     /locus_tag="IVW29_001364"
     CDS             19682..19966
                     /locus_tag="IVW29_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7838854.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20017..21642
                     /gene="groL"
                     /locus_tag="IVW29_001365"
     CDS             20017..21642
                     /gene="groL"
                     /locus_tag="IVW29_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7838855.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7486 a         3506 c         4373 g         6444 t
ORIGIN      
        1 aacaatatga acagcaattg aaaagaaaag aattggttta tctaaaaaaa tatactgatt
       61 cactggaaaa agatcaagaa aaatttgcca gatttagaca tgactataaa aatttattgc
      121 tgagtttaaa agaaatcgct aatcaaaacc atgattcgat attggaaaaa gaaataaaag
      181 aattagagat ctattctaat tcttatctaa ccaataagtt tgaatatcgt tatttaaaaa
      241 atataaaaaa tgaatatttg aaaagtttgt tgatagcgaa gctataccaa gcaaataaca
      301 ataagatttg ttgtaaattc gaatgtccaa gtactattat tgaagttcct gttccaatat
      361 ttgactgtat tagggtgctt ggaattattc tcgataatgc aattgaagca tctatagaga
      421 gtcaagatag agaactgtct ctagctattt accgagatga taaacagata gaaatttcga
      481 tatcgaatag ttgtagagag atactactct ctatagatac tctaatgaaa aaaggaataa
      541 caacaaaaaa gggacatcaa gggttaggtt tatctagtat agaggaaatc aataaaaaga
      601 atcccaatat gtttgtgaac tatcaaaacg atccgtatca atttactact caagttattt
      661 tgttgttttg agggaaggag gatttatgag ttatccaata atattatgtg aagatcaaat
      721 tatacaatta aatcagttag aacgaattat tgataatttc attctttttc acgataaagt
      781 atttaagata gtattgaaaa cacaaagtcc gttagaagtt aaaaaatacc tcaaacaatt
      841 ccgaccaaag caaggaatat atttcttaga tattgattta aatcatgaag ttaacggtat
      901 agaattagca gaagtaatca gaaaatatga tgttcaagca aaaatcattt ttacaactac
      961 tcatgatgag atgttacccg taacaataaa aagaagagtt gaaacgttag gatttgtaac
     1021 aaaagatcaa acactagatg agtatcgaaa cgagattgtt gagttattgt tattagcgca
     1081 agaaaggata gatgcaacaa aacaggttac gaatcaagca tttgtttttt ccataggttc
     1141 tcaaacattt acttttgata taaatgacat ttattttgta gaagcttcaa aattaccaca
     1201 tagattatca ttgtgtacta aagatgggca gtacgaattt tatggaagaa ttagtgagct
     1261 tgaaaagaaa tatcctatgt taacaagaat tagtcgagcg tgtttagtaa atatctttaa
     1321 cgttaaagaa atagatttta aaaaacgttc actatatttt gattcagagt tggccagaaa
     1381 ctttacttta gggaaagcac aaaaaattaa ggagaagttg aaagaaagca ctgtttagtg
     1441 gttttttctt tagggagatt gcatttttct aataaaatag attccttcac tagtgcaaat
     1501 ttttctgaat cctattaaag ttgtaccaat ttttatagct gttattagtt acgtgcataa
     1561 cagcaatatt agttactgga ggaatatttc tatggtctgg aaccaaaaaa gggccaaatg
     1621 aatagttctt ttcttgtttt tattaagtac cataaagggg aattaaaatt aaatgtatgg
     1681 tggaagatgc gaaagatttg gcaaattcta ctaattttta gaaagtgttt ttcatttttt
     1741 agttaaaaaa tttgtcctgt caagtgcaat cctatgattg tacttgatag gatttgagat
     1801 aattactttc ctgtttttat aaatttaact tgcaggaaca ggctttaatg cacatccgac
     1861 aagtacatta ccaatacctc ctgtaacggc cgaaataggt cctcctgcag caccaactac
     1921 agcgctgcaa gtccttttgt aaaaatatct ctaccattac atttccaaag aatttttatc
     1981 tgactttaat aaaagataaa aatttaatat aattataaaa actagcacaa gaagctttag
     2041 taaaaagttc tgtccattcc aaacttttga aaagataaag aacataccta tgttcgtaat
     2101 cgacacaaac caatcttgag gtttgatatt ttttaattgt ttacgcaccg gtccacatgc
     2161 tgaatccagc acctaataaa gctccaccta caccacaagc agctattgca taaggtcctc
     2221 cggctgaaca aagttttact cctgctgttg ccccagaaaa agtagtgcct aaaactccca
     2281 cggcactccc accttgaata tttttcaact cttctgaaga taatataaat gttgtttttg
     2341 tgtttttaat atgcatacta cttattctcc ttttttattt ttttcgagca tcccagccag
     2401 ctgcagcacc tccagcgaca gctccatata accctccaac aacaccgcaa gctggtccgc
     2461 aaattgctgt tcctaaacct gcaccaccta ctgcccctgt ggtaagtcca cctacaactt
     2521 gccccaattt tccccaagca ttattgcctc caccaacagt ttgtgcattt ccacaatact
     2581 tagttccttt acattttgca tgtttaaaca cgcttcttta aataatattt tttacggtgc
     2641 tactgtaaca tacgtatttg attaacagta aaaatcttcc tgaaacttat caagtttatt
     2701 cttgaatagt aaaatttcca aaataaattt gattcaggaa tgaaaaagtt agtgttcaag
     2761 aactgttttc aaagtttttg tggaattatg agacaatatt ttcactgtat taaagctata
     2821 ttgagaattt ttctataaaa attttttaga aggagttgtg ttagataagt gataaaatca
     2881 tttccttttg tgcagcaaca agatgaaaaa gattgtggtg ttgcttgcct ttcgatgatt
     2941 ttaaaatatt ttaaaactga tatcccgatt cataaattac gggacctatc tgggactgat
     3001 ttagagggta cttcagcatt tggattgaaa aagacatttg aaaaattgtc ttttgattgt
     3061 atggctattc aagcagatag tgagatctgg aaagaaagag atcttatctt accattaatt
     3121 gcacatatat tggttgataa cagctatatg cactacgttg taatttataa aacgaaggga
     3181 aacgtactat ggattgcaga tcccgctaat ggaaaaaata gaaaaacgat tgaagcgttt
     3241 caaaaagaat ggacaggtat tcttttatta cctatgccta gagaaactta tattcctatt
     3301 aaagaaaaag tagctggatt aagttctttt ttgcctataa tttggaaaca aaaaggatta
     3361 gtattccaca tcgttttagc atcgattttc attacgtttt ttggcatagg aagctcatat
     3421 tattttcaag gtattcttga ttttttcatc cctaatcaag cacgttcaac tttaaatata
     3481 atttccattg gcttgatcgc tgtctattta tttcgggtat tgtttgaata tagtcgtagt
     3541 tatcttctta ttattttagg acagcgaatg agtatggcaa ttatgcttca gtatttcaaa
     3601 catgtgttaa ctttaccaat gaattttttt gctacgagaa aatcagggga aattatttct
     3661 cgttttcttg atgccagtaa gattattgat gctttagcaa gtgctacgtt atcaatattt
     3721 ttagatattg gtatggtgtt gcttgttggg gttacgttag ctattcagaa tagtacgttg
     3781 tttttaatca ctctatgctc tttgcctttt tatgtagttg caatattcgc tttcgtgaaa
     3841 agctatgaaa aatcgaacag ggaagaaatg gcagccggtg cagctgtaaa ttctagtatc
     3901 atagaaagtc ttaaaggaat agaaacaatt aaagcatata gcggtgaaga gaaagtatat
     3961 aaccgtatag accatgaatt tgttaaatta atgaaaaaat catttcgtac ggttacgctt
     4021 gataatattc aacaaggaat taaacatttg attcaattaa ttagcagtgc attaatttta
     4081 tggctaggtt cttattttgt tatggataat agaattagtc taggtcaact tatcacatac
     4141 aatgctttac tagtattttt gacagatcct ttacaaaaca ttattaattt acaagtgaaa
     4201 atgcaaacgg ctcaagttgc aaataatcgt ttaaatgaaa ttttcgcgat tgattcagaa
     4261 aaatcgaacc agtcttttga atatatgctt tctgaacaat tatttcaaca agatatcact
     4321 gtaaaaaacg tttcattctc gtatagcatg aaggccccaa cgttgaacga aatttcatgt
     4381 acaattcctt cgcacagtaa aacggctata gttggagtta gtggttctgg aaaatcaact
     4441 cttgcaaagt tatttgttcg tttttatgaa gcatcagaag gatcgatcca ttatggaaac
     4501 attaatcact tagacattcc ttttcagata ttacgcgatc gagtgacata tttgcctcaa
     4561 gagtcatttt tctttagtgg aacaatcatt gagaatctta tattcggttt agatagccca
     4621 ccaagtttcg aacaaataat agaagtttgt gagatagtcc aactaaatga atttattaat
     4681 caacagccat tacgttacga gacattgttg gaagaaggtg gaaataatct ttcaggaggg
     4741 caacgacaac gattagcctt agcacgggct ttattaaaaa atccggacat attaatttta
     4801 gatgaagcaa ctagtggatt agataccttg ttagaacatg caattatgaa ttatctcctc
     4861 aatttaaaag aaaaaacgat tatttttatt gcgcatcatt taccaatagc aaaagcatgt
     4921 gatcaaattt tagttctcca tgaagggcat ttggtagaaa aaggaacgca cgatgaactt
     4981 cgttttgccg atggtatcta tcaaaaatta tgggaaatct aggagaggga aacaatttat
     5041 gcagaataat aattggttcg aacattctag tatctatact aaacaacatc atagcttcta
     5101 ccgttgggtg ttataccctg taattctttt tttatgtatt attggattgt ttttaacctt
     5161 tgctaaaaaa gaggttgtta ttcggacaac ggctcaattg aaccccgaaa aaatagagaa
     5221 gttacaagtg ccattagaag ctaaaataat ggaaaacttc ctaaaagaaa atcaatttgt
     5281 tcataaggac gatgtgttgg ttagattgga ttgttcttta atagagaatg aaaaggctca
     5341 gatagaacaa gaaaaccaac gtattactca acaaataaag atggctcagc tatttattga
     5401 aagtataagt aaaggaaaaa atttgttttc aacggatgac agttttggct acagtaatca
     5461 attaaagagc atgttgtcag aaaaagaatc actccgctac gctttgaagc aaagtgaatt
     5521 aaatgatcaa aagcaattag aagtatacga aaagacaaaa agacaactag aaaaacaaat
     5581 tgagagttca gatagtaaat tacaagaatg gcaacaagta caggtagctt ggagtaataa
     5641 tcaatcatta aaagattttt caaaagaaat gatggcaaac tatgagaatt ggcaagaaca
     5701 actaaataat gtttctgatg atcaaaaaaa tcaagtgaaa ctgacaattt cagcaagcat
     5761 aaatgaacaa attgagcaac taaaaaaaga agtagaacag tatcagtcag aaaaagctaa
     5821 attagttaaa ccaactactt ctgagaatga cagaattagt caaacggaaa aaggaaagca
     5881 agagctagaa caagcaataa caactgtaaa acaaacagtt acagagctaa atgaaaaaca
     5941 agagaagaac cagtcagtta tcaaaagttt ggacgatcaa ctttcaaaag gaatactaaa
     6001 agctcctgta tctggaacgg ttcatttaaa tgaagaaaca aagggacaag cagaactagc
     6061 caaaggaaca gtgcttgcag aaatttatcc gatccacgaa aaatcaaaaa tgaaatttac
     6121 agcactttta cctgcgaatg aaagtaccta tattaaagaa gggatgaagg ttcattttaa
     6181 attggatcaa aaaggtgggg ctccgattac gatcgacgga actttagatg aaatttcaga
     6241 aaattctact gccactgaga aaggtgtttt ttatgcagtg aaaggaacgt taaaaacaac
     6301 tcaatcgttt ccgtttcgtt atggattaac tggagagttg tctttaattg taggagaaaa
     6361 aacatattgg caacaaattg tcgatgtgtt tctaaataaa aaataattat ttttactaag
     6421 aatataggag gagtttatat gaataaaaga agatggattg ctgtgggagt agcgtgcgga
     6481 ttattgttac tatccattat cgtcgcgcta attccaggaa aagataaaga ggaagcatca
     6541 aatacaaccc taacaggaat aaataagatt ttttacggat ctaacgaaat cacagaagaa
     6601 acgttagaag aaggcgcatc aaataaaaag attgtcaaac tatccgtcaa tggagttatt
     6661 gcagatacag gtgaaagcaa tctttttagt agggaacaat ataaccatca gaatttttta
     6721 acacagctaa agaaaattca agaagacaaa gcggttaagg gagtgttatt agaagtaaat
     6781 tcacctgggg gaggaatata tgaaagtgct gagattgcta aagaaatggc aaaaattaaa
     6841 aagctagata ttcctatcta tactgcattt aaaaatacag cggcaagtgg agggtactac
     6901 atttctgctg gttcagataa aatatttgct acggaagaaa caacaactgg atcaattggt
     6961 gtcattattt ctggtttaaa ttattctggc ttattagaaa aacttggcgt aactgatgcg
     7021 acgtataaaa gtggtgcatt aaaagatatg atgtcacctc aacacaaacc ttcagaggaa
     7081 gagaataaag ttatacagga atttgtgatg agtgcttatg atcgttttgt caatgttgta
     7141 gcaaaaggaa gaaatatgga tactaatgca gtgaaagaac ttgcggatgg aagaatttat
     7201 gatggtaacc aagcagtaga aaatgggttg gtggatcaaa ttggttactc tgaagatgca
     7261 ttagattccc taaaaaaaga gaagaaatta actgatgcga cggttataga atataaaaat
     7321 gacacaactg gctttgcttc ttcttggtta ggtaataaga ttgctgaatg gcaagggcta
     7381 aaggcaacta catcagatcg tgtaatttca gtttttgaaa aacttggatt ttctgatact
     7441 ccaaaaccaa tgtattacta tggagggcta taaaatgaat aagaagagtg gcattgaaac
     7501 tttgcctaat tatgtatttg cgggattttg gttacgattt tttgcttttc ttattgattt
     7561 aatttgtatt agcatcatca cccatgctac tataggtctc ttttacaaaa tcatgggact
     7621 ggaatctgac aaatccatta ttacttttta tggtatagcg tcattagcta tttatttaac
     7681 ctattttgta cttttgacta agctgaattc aggacaaaca attgggaaaa tgatttttgg
     7741 gataagagta gtgagctttg aggaaaaaga gctaagttgg caaactatct ttattcgtga
     7801 gtgtatatgt aggtttatat taaaaaattt tgtatttcaa attgggtatt tggtggctgc
     7861 tttcacgccc aagaaacaac atattgggga ttatttttct aacacgtcag tggttgttat
     7921 tcgttcatta gaagctatta actatggtca aaaaatttct aattctaaaa cacttaatct
     7981 ggagagaatt aaatgaataa gagaaaaaca gagtttattt tgattttaat tagtggaata
     8041 acagggattt tatgtagtct tttagcattg atgggtggta tgtttctatc ttctattgat
     8101 tcaactactt taaatactac agaactaacc agtaaggata tagatatgtt agtcaatcta
     8161 agcgggaatg ttgttttatt tggaggaatt accctagttt tttcgataat gattttcata
     8221 ataggttttt tagtaagaaa aaatcaaaaa acagtggtct atggtattag tttactcatt
     8281 ttatctatta ttccattttt tcttttaaca ttattgtggg taattcctgg agttttaggt
     8341 attattgcgg gattgatgct tctaataaga aaatcttaat taggcaatgt gtagacaacg
     8401 tgaaacgatt aatcaattaa aagctaaata cgttttgaga aatgataact tgtagtcgat
     8461 ggaagacatg tagataaaaa aactacgtgt cttttattta ttctaatttt ttcgaaaaaa
     8521 agacactctc tgattgttgt attaatggaa aaggggaaag cagcaagtaa atttagtttt
     8581 tctattaaat attcttcact ttttaacctt ttatagtact tagatttaat cctatatgtt
     8641 tagagagttc attggcagca tgattttgca aagcttctcg aattattgtt atattagaaa
     8701 ctttactgtt cataattatg cttctttttt aactcaaagt ccaaagggct acagtggact
     8761 ttgagttaaa aataaaaagc cctagttatt ttgaaaaata aaaaaagaac agcgtaaatg
     8821 ttttcgaaat gctttcgcac caacatttac ctgttccgtg ggtttggcac ccaatgggcc
     8881 gtgaggggct cgaacccgcg acccgctgat taagagtcag ctgctctacc aactgagcta
     8941 acggcccgta caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta
     9001 catttaaaga aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc
     9061 acatttgctc gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc
     9121 ctaacacttt tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct
     9181 taacgataaa agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta
     9241 atcaaatcac ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt
     9301 tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg
     9361 gggaaggaac tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt
     9421 tatatggacc aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa
     9481 aagcatccat tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca
     9541 gcaccagtta aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg
     9601 ttgaaacaag tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca
     9661 gtaggaattc gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg
     9721 gtcagccaat tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta
     9781 gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa
     9841 acgattgttc tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat
     9901 cgcaatttta gtagattcag gtacggatgt tccagaagaa ttgataaaaa aatacgagat
     9961 gtatatgatc ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat
    10021 tacccctgaa gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga
    10081 tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact
    10141 tgcggtaaca atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca
    10201 acagcaagaa gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg
    10261 tttttctgcg atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat
    10321 cagtaatctg aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga
    10381 atatcttcaa aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa
    10441 gcttaaccca atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag
    10501 gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc
    10561 aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga
    10621 agcgagactt aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc
    10681 ggcattagta gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg
    10741 ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt
    10801 agaacaaaaa ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag
    10861 gcaaagatga aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa
    10921 gaggtggctc tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat
    10981 cgtcaaattc aatttagcca aagaaggcta tgacgtttat actgcatatg atggggaaga
    11041 agccttggaa aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc
    11101 taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat
    11161 tatggtaaca gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga
    11221 tgattacgtg accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct
    11281 tcgtcgcggt gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat
    11341 tggtgatttg acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga
    11401 gttaacacat cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat
    11461 gacaagagaa catttattac aaacggtttg gggttatgat tattttggag atgtacgaac
    11521 agtggatgta actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa
    11581 ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt
    11641 ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta
    11701 ttctgatttt attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac
    11761 gttccacgat caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa
    11821 caatcagccc ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata
    11881 tcaaaagatt tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg
    11941 aaaaagggat catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa
    12001 atgattatgt tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg
    12061 ataaacgggt gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg
    12121 gggctcttta cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag
    12181 tcattttctt tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag
    12241 ttgctcgttc gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta
    12301 gaggagacta cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg
    12361 ataccttcaa tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac
    12421 gcaatcgttt ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca
    12481 ggggtaaagt cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg
    12541 aagcaatcgg acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga
    12601 aattattaga agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg
    12661 atcagatgat cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg
    12721 gattagtagc tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg
    12781 aattcgtctc taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata
    12841 tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg
    12901 taacgctaga tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc
    12961 gcatggatac aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga
    13021 attttgttct tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc
    13081 gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag
    13141 tagtcgataa tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt
    13201 gtcgcttatt agaaacgcat aataacgtga ttttaagtat caccgatcaa ggattgggga
    13261 ttcctaaaaa agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg
    13321 caagagcaca aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc
    13381 atcgaggcgc tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc
    13441 tgccatatga accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg
    13501 tataggactc attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag
    13561 cccagcagga aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca
    13621 aagcaatcga aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg
    13681 aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa
    13741 agcaagattt ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt
    13801 gaaaaatctg aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata
    13861 caaggaagca tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat
    13921 gtattttact agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc
    13981 gaaccatctc gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt
    14041 aacagcaaaa gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca
    14101 atatgctacg aaacattcga tcaaactgaa gaagtatagt tatatcctgt cacagcaacc
    14161 ctatacactt tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga
    14221 aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca
    14281 agtagttgat tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga
    14341 aagtttcccc tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg
    14401 cagtgacaat acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga
    14461 cagcagagga cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa
    14521 tatccaaacc agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact
    14581 tgcaagtacg aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat
    14641 tggttcagtg atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga
    14701 tctcttacct gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct
    14761 ggaaaaatta cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt
    14821 tttcgtcgct tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata
    14881 ccaagaaaac aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag
    14941 cgacgatatc acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag
    15001 cgcggagcag accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg
    15061 cttgctggca agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc
    15121 agatgctgaa aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt
    15181 atctaacaaa gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca
    15241 tttagaagaa gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa
    15301 tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac
    15361 gaaatacatg cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta
    15421 tacggaagag gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa
    15481 aattttatgg cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta
    15541 tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt
    15601 caacgctttc agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata
    15661 aaaaagcgag tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc
    15721 tgaagaaaaa ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact
    15781 ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat
    15841 cacttccttg atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac
    15901 gcatgaacat cgagatcata taaatggcgt gggtgtcttg gctcgtaagt atcatttgga
    15961 tgtttttgcc aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt
    16021 ggaacaaaaa catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga
    16081 gagtttcggc gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa
    16141 tcggtcattc gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat
    16201 tgaaaatgcc gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg
    16261 accttatccg tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga
    16321 agacggtgca cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg
    16381 ccatcttagc aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt
    16441 gaaagaaaaa gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga
    16501 agcaactgag ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga
    16561 taaaatcaat cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat
    16621 aaccgtatta tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct
    16681 catctactaa tgcaatcgcg taatctgcca tgctgatcgt actgtcgcta gcagcgtttc
    16741 tttgtaaata atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata
    16801 aagaagctgg actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc
    16861 cttcgtacat attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca
    16921 tacgagtcgt cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc
    16981 gtgtatcggt tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt
    17041 cttttccttt gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag
    17101 ttgtcaactc aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat
    17161 gacgcacgat tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac
    17221 ctgcatggcc agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct
    17281 gtccattgta agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg
    17341 cgcattcctt tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata
    17401 aaaatagaag ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa
    17461 tgataacatc tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca
    17521 cgatcccaat aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt
    17581 acatcgctca gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg
    17641 ctacatttga tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag
    17701 aagaagacat cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta
    17761 ttcagccact aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag
    17821 cagttacaac tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag
    17881 ggtggaagca aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc
    17941 caatcattgt tatagtcact tttacgctta tcggttcagc ggtttttcaa catttgttca
    18001 gaatgactgt tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag
    18061 acacgttgtt tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc
    18121 ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag
    18181 caaatgcaga aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg
    18241 ttactcagca atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg
    18301 ctgtattttt tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg
    18361 ctcttttacc agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga
    18421 tgaacccgat gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct
    18481 attccgcttt atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa
    18541 caccgccgat cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta
    18601 tcgtgttgtc aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa
    18661 actgcaaaaa ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa
    18721 aaacaaaaat actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt
    18781 atttatagtc atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat
    18841 gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct
    18901 aggctgaaga agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac
    18961 gcaaacagca aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc
    19021 cccagtatac tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc
    19081 aaaataaaag cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg
    19141 gttgctttgc caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg
    19201 acaaagattc caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct
    19261 gattcgatcg ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct
    19321 agtatataca tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc
    19381 gaagctagga tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta
    19441 tattttttta aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct
    19501 tatatcgatt gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc
    19561 ttttctttga tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg
    19621 ttagcactcg aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat
    19681 cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac
    19741 tgtcggagga atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt
    19801 tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc
    19861 aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga
    19921 atatctgatc gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa
    19981 aaaataatca aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca
    20041 gaagacgcac gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta
    20101 acattaggac ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc
    20161 acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg
    20221 ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca
    20281 acgacagcaa ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca
    20341 ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa
    20401 gaattgcata atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct
    20461 gtttcttcgg gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga
    20521 aacgacggtg tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta
    20581 gaaggtatgc agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa
    20641 atggaagctg ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc
    20701 caagatattc tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt
    20761 gctgacgatg tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca
    20821 ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa
    20881 gatatcgcta ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa
    20941 gatgcaacaa tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca
    21001 acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa
    21061 aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct
    21121 aaattagcag gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa
    21181 gaattaaaat tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga
    21241 atggtctcag gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa
    21301 gcagaaggag acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt
    21361 cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac
    21421 gtggaactag gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca
    21481 ggtatcgtag acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct
    21541 gccttgttat taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca
    21601 gctcctgcaa tggatccatc aatgatgggc ggtatgatgt aagaaaaagc aacttgctta
    21661 tctacaactg atcattagtg aaagacagat aaaaagaggc tggaacagaa gcgtttaact
    21721 ccaagaaata agaagaaatt cacggaaatt gcttttcaaa tttttgtgaa tttcagctta
    21781 tttccgaagg agttgcttct gcttccgct
//
LOCUS       DACCVU010000042        21807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCVU010000042 DACCVU010000000
VERSION     DACCVU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.042"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IVW29_001366"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IVW29_001366"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7838856.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IVW29_001367"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IVW29_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7838857.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IVW29_001368"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IVW29_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7838858.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IVW29_001369"
     CDS             complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IVW29_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7838859.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IVW29_001370"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IVW29_001370"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7838860.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IVW29_001371"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IVW29_001371"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7838861.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="IVW29_001372"
     CDS             complement(8558..9028)
                     /locus_tag="IVW29_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7838862.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="IVW29_001373"
     CDS             complement(9074..9499)
                     /locus_tag="IVW29_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7838863.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="IVW29_001374"
     CDS             complement(9566..9835)
                     /locus_tag="IVW29_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7838864.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="IVW29_001375"
     CDS             complement(9846..11441)
                     /locus_tag="IVW29_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7838865.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IVW29_001376"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IVW29_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7838866.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /locus_tag="IVW29_001377"
     CDS             complement(14994..15554)
                     /locus_tag="IVW29_001377"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7838867.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="IVW29_001378"
     CDS             15907..16881
                     /locus_tag="IVW29_001378"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7838868.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="IVW29_001379"
     CDS             17051..18451
                     /locus_tag="IVW29_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7838869.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="IVW29_001380"
     CDS             complement(18529..19251)
                     /locus_tag="IVW29_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7838870.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="IVW29_001381"
     CDS             complement(19330..19836)
                     /locus_tag="IVW29_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7838871.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="IVW29_001382"
     CDS             19959..21758
                     /locus_tag="IVW29_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7838872.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6054 a         4752 c         3910 g         7091 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 aaagagttag tccagaatgg gctaact
//
LOCUS       DACCVU010000043        21489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCVU010000043 DACCVU010000000
VERSION     DACCVU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.043"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(75..593)
                     /locus_tag="IVW29_001383"
     CDS             complement(75..593)
                     /locus_tag="IVW29_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7838873.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(595..1212)
                     /locus_tag="IVW29_001384"
     CDS             complement(595..1212)
                     /locus_tag="IVW29_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7838874.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1209..2072)
                     /locus_tag="IVW29_001385"
     CDS             complement(1209..2072)
                     /locus_tag="IVW29_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7838875.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(2059..2970)
                     /locus_tag="IVW29_001386"
     CDS             complement(2059..2970)
                     /locus_tag="IVW29_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7838876.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3011..4345)
                     /locus_tag="IVW29_001387"
     CDS             complement(3011..4345)
                     /locus_tag="IVW29_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7838877.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4553..5383
                     /locus_tag="IVW29_001388"
     CDS             4553..5383
                     /locus_tag="IVW29_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7838878.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5627..5876)
                     /locus_tag="IVW29_001389"
                     /pseudo
     CDS             complement(5627..5876)
                     /locus_tag="IVW29_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6067..6261)
                     /locus_tag="IVW29_001390"
     CDS             complement(6067..6261)
                     /locus_tag="IVW29_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838879.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6766..6951)
                     /locus_tag="IVW29_001391"
     CDS             complement(6766..6951)
                     /locus_tag="IVW29_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838880.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7801..8142)
                     /locus_tag="IVW29_001392"
     CDS             complement(7801..8142)
                     /locus_tag="IVW29_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7838881.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8142..8372)
                     /locus_tag="IVW29_001393"
     CDS             complement(8142..8372)
                     /locus_tag="IVW29_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7838882.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8659..8997
                     /locus_tag="IVW29_001394"
     CDS             8659..8997
                     /locus_tag="IVW29_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838883.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9202..9441)
                     /locus_tag="IVW29_001395"
     CDS             complement(9202..9441)
                     /locus_tag="IVW29_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7838884.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9417..9764)
                     /locus_tag="IVW29_001396"
     CDS             complement(9417..9764)
                     /locus_tag="IVW29_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7838885.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10496..11077)
                     /locus_tag="IVW29_001397"
     CDS             complement(10496..11077)
                     /locus_tag="IVW29_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838886.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11260..11886)
                     /locus_tag="IVW29_001398"
     CDS             complement(11260..11886)
                     /locus_tag="IVW29_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7838887.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11908..13239)
                     /locus_tag="IVW29_001399"
     CDS             complement(11908..13239)
                     /locus_tag="IVW29_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7838888.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13394..13795)
                     /locus_tag="IVW29_001400"
     CDS             complement(13394..13795)
                     /locus_tag="IVW29_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7838889.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13881..14087)
                     /locus_tag="IVW29_001401"
     CDS             complement(13881..14087)
                     /locus_tag="IVW29_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838890.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14182..15684)
                     /locus_tag="IVW29_001402"
     CDS             complement(14182..15684)
                     /locus_tag="IVW29_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7838891.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15702..16157)
                     /gene="argR"
                     /locus_tag="IVW29_001403"
     CDS             complement(15702..16157)
                     /gene="argR"
                     /locus_tag="IVW29_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7838892.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16255..16872)
                     /locus_tag="IVW29_001404"
     CDS             complement(16255..16872)
                     /locus_tag="IVW29_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838893.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17093..18466
                     /locus_tag="IVW29_001405"
     CDS             17093..18466
                     /locus_tag="IVW29_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7838894.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18541..19653
                     /locus_tag="IVW29_001406"
     CDS             18541..19653
                     /locus_tag="IVW29_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7838895.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19725..21362)
                     /locus_tag="IVW29_001407"
     CDS             complement(19725..21362)
                     /locus_tag="IVW29_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7838896.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         6289 a         4397 c         3731 g         7072 t
ORIGIN      
        1 tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta
       61 ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact tcctaaaata
      121 gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc
      181 aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct
      241 actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt
      301 gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg
      361 ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat
      421 tctctagtat caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca
      481 ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc
      541 ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc
      601 ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa
      661 caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag
      721 aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa
      781 aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa
      841 gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc
      901 cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc
      961 taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga
     1021 accgagcttt ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact
     1081 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
     1141 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
     1201 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
     1261 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
     1321 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
     1381 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
     1441 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
     1501 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
     1561 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
     1621 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
     1681 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
     1741 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
     1801 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
     1861 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
     1921 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
     1981 attcctatta ctgtcatgag gacataactt agtatttttt tgtttgccga ttcacactgg
     2041 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
     2101 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
     2161 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
     2221 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
     2281 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
     2341 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
     2401 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
     2461 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
     2521 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
     2581 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
     2641 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
     2701 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
     2761 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
     2821 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
     2881 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
     2941 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
     3001 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
     3061 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
     3121 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
     3181 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
     3241 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
     3301 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
     3361 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
     3421 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
     3481 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
     3541 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
     3601 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
     3661 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
     3721 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
     3781 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
     3841 atatcttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat
     3901 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
     3961 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
     4021 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
     4081 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
     4141 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
     4201 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
     4261 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
     4321 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg
     4381 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
     4441 aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta
     4501 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
     4561 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
     4621 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
     4681 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
     4741 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
     4801 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
     4861 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
     4921 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
     4981 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
     5041 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
     5101 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
     5161 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
     5221 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
     5281 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
     5341 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
     5401 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
     5461 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
     5521 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
     5581 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
     5641 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
     5701 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
     5761 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
     5821 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
     5881 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
     5941 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
     6001 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
     6061 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
     6121 agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
     6181 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
     6241 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
     6301 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
     6361 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
     6421 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
     6481 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
     6541 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
     6601 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
     6661 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
     6721 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
     6781 aaaataacca acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat
     6841 aaatgttgaa aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata
     6901 ccctataaaa gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca
     6961 cttccttaaa taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga
     7021 ctcaaaataa aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa
     7081 agctctactg acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt
     7141 aatcaattag agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg
     7201 agattctact ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg
     7261 gtgtcgttag aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt
     7321 ctgtatggat gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat
     7381 atgccttttt gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc
     7441 caaattttac gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag
     7501 tttagatttt ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt
     7561 gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa
     7621 aaacgaatga cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag
     7681 tttaagatac ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg
     7741 ccttttttta atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc
     7801 ttaaaacata tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc
     7861 tatttttttg agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg
     7921 tccttttata tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga
     7981 agtaattgga catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac
     8041 tagtcctggt cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac
     8101 aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct
     8161 tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta
     8221 aatggatctt caatttttgg aactaacgta attgttccat catcagaata cacaacgata
     8281 tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa
     8341 cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac
     8401 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
     8461 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
     8521 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
     8581 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
     8641 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
     8701 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
     8761 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
     8821 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
     8881 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
     8941 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
     9001 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
     9061 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
     9121 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
     9181 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
     9241 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
     9301 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
     9361 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
     9421 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca
     9481 caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg
     9541 gcattaatca tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga
     9601 gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct
     9661 ccagctgcta attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc
     9721 tgaaggagct ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat
     9781 ctttttttct atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt
     9841 gtagaactaa aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca
     9901 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt
     9961 ccttgtgact cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc
    10021 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt
    10081 tttcttaata ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
    10141 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt
    10201 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctccact aaacggtgtt
    10261 caatcagcta accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    10321 gtttgtttct ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt
    10381 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt
    10441 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa
    10501 gaaccttttg cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg
    10561 atagatcctg tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt
    10621 tggcttgacg gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt
    10681 gtttcgtata cttgacccat tgattgacta attctagtag caacagacgg tattcttcat
    10741 ccactgtact caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg
    10801 tctcatgatc agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc
    10861 cagcatttaa gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca
    10921 ccgctagaca aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat
    10981 cagaagctgc tagaatatct cggattgacg tttcatgata gcctttttta taaataagat
    11041 ctttggtcgc ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct
    11101 catagtccta tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac
    11161 tgtgccaaat tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc
    11221 attgattcaa gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac
    11281 ttcctctgtt gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat
    11341 tgcttcttgg ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc
    11401 taccaattca cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac
    11461 tgtgtcgatt ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat
    11521 tttatgagga gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa
    11581 atcggctcct gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg
    11641 ctcaagcatc atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa
    11701 ataatgagga gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat
    11761 catattttcg atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc
    11821 tgctggtttc aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt
    11881 gaacatggtg gattctccat tcgttattta ttttaataca tcatttagag cttcactcat
    11941 tgtcggatga gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc
    12001 tagcgaaatc aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat
    12061 caagtcagtt tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg
    12121 tgctttaggg acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt
    12181 ggcttgtttt tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg
    12241 cacagctttc cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat
    12301 tcgataatca tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc
    12361 ccaaatgttg tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt
    12421 tagtttaaca tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc
    12481 agccaaaact tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc
    12541 agctgttgat tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc
    12601 cgcatttgtc atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa
    12661 ttcagaatgg gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa
    12721 ttccaagcca atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc
    12781 catcgcttgc gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat
    12841 gattggttca gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc
    12901 attattcgtc acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt
    12961 cccagttgcc tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt
    13021 ttctttccga gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga
    13081 tttagaagga atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc
    13141 aacgactaaa accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt
    13201 tccaaaaccg atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta
    13261 cctaccgatt ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct
    13321 ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat
    13381 ttcctttatt cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg
    13441 agtaagggca gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc
    13501 catcgatatg cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa
    13561 gagagacacg tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt
    13621 gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc
    13681 cttctgcatg gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga
    13741 cttctccatt gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac
    13801 tgctcctttc gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc
    13861 gctttctaca aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca
    13921 aaaaacaaaa aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca
    13981 atcttttcta cataatcttt tatatgattg attttaagat acatatatac cagaaagttg
    14041 accccataaa ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt
    14101 tgctggtctc cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac
    14161 tagcctttca aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc
    14221 gaataatttt gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat
    14281 ctgtaatact agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta
    14341 tggctcttct agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt
    14401 ttcttcttcc aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc
    14461 atcgctgaac aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat
    14521 ttgttcatct aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc
    14581 gatgacatat gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt
    14641 ttcgatggct agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac
    14701 atttcgatag ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg
    14761 tagatgagaa gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac
    14821 gattgtactt aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac
    14881 aactgcagaa cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa
    14941 aacagcctgc ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg
    15001 aaatcggcca tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa
    15061 tactttttca gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc
    15121 atttagtggt ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg
    15181 cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt
    15241 ttgctgctgt gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa
    15301 gaattttatt ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc
    15361 gcacgagaac tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa
    15421 atcgatgaca tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg
    15481 tccgcttatc ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga
    15541 tttcttcttt ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt
    15601 cgtcaagttt acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt
    15661 ttgcagcgct ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc
    15721 agcttttctg caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct
    15781 acaccggcca tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc
    15841 acatcggcac catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc
    15901 aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg
    15961 ttaaataatg tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct
    16021 cgaatgtctc tagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca
    16081 agcagctctt cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt
    16141 cgctgtttct ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat
    16201 atttcttcat attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc
    16261 tttttctatt ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc
    16321 gctgatgtaa atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa
    16381 tgcttctaat ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg
    16441 ggcacaaaga ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag
    16501 tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg
    16561 gatcatcaat tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc
    16621 ttgcagtccc cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag
    16681 ccaatttttt ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct
    16741 tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt
    16801 aggaacctct agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc
    16861 aattttttcc atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct
    16921 ttttgagtga cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga
    16981 agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt
    17041 ttataataga aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa
    17101 acaaaaacag ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg
    17161 cgtattgatc gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt
    17221 aacgactgca caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt
    17281 tccgctgtct acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa
    17341 tctgcttttc ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt
    17401 gtttggtcga ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat
    17461 tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac
    17521 aacggccatt gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac
    17581 atggaatcat atcttcctgt ttcttgtgcc cgttttgttt ctttctttga ttatcggctg
    17641 gtttgctatt cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt
    17701 aggaatttgt cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc
    17761 cagctggatt gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa
    17821 aaaagtggag catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt
    17881 tttgattggc ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa
    17941 tttccttcag atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg
    18001 cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc
    18061 gaagaagcca atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct
    18121 actcatagga cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg
    18181 tatcggatta tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact
    18241 acaaggagac gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac
    18301 ttcattagta gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt
    18361 gtctactaca ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag
    18421 ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc
    18481 aggtgttgca tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt
    18541 atggaaaaaa ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta
    18601 gatacatcaa aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct
    18661 agcgctggga ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg
    18721 gctaaagacg gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg
    18781 atttacgcac aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct
    18841 gttcttgccg agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca
    18901 cctgaacaga catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt
    18961 ggtggtggtc gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa
    19021 aaacaaatgg agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc
    19081 atcaagggac cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca
    19141 cttaagcctt tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag
    19201 ctcttagcat ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac
    19261 ggagaagctg atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa
    19321 aacgatcaag gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt
    19381 acagaacagt tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta
    19441 ggtacccgtg cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca
    19501 agcttactgg ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc
    19561 tatctattag ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac
    19621 atctccacct atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc
    19681 cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga
    19741 atcaaattct ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct
    19801 tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc
    19861 tcaaaagaaa caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac
    19921 gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt
    19981 ttgatttttt tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc
    20041 aaaaacaact ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta
    20101 gcaaatggtt caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct
    20161 gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg
    20221 ataattggcg gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta
    20281 agatgatcga ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt
    20341 cgtatatctc ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc
    20401 tgacaagtaa ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca
    20461 aagtaagaag gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg
    20521 tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt
    20581 ccagaaccgg acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt
    20641 ttcatcccac cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc
    20701 aaagtcgctt tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg
    20761 agagcatcaa acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa
    20821 aagatataaa gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc
    20881 gaattcgctg gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga
    20941 atcagcaaat gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg
    21001 taaaactggc gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt
    21061 tgttccttgt ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt
    21121 ccatcattga atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag
    21181 attgttaaat ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc
    21241 ctatcaaaaa cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata
    21301 ctggataaca gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc
    21361 atggctcctc ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt
    21421 tttatttgta ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga
    21481 gaacttgaa
//
LOCUS       DACCVU010000044        21294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCVU010000044 DACCVU010000000
VERSION     DACCVU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.044"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..373)
                     /gene="trxA"
                     /locus_tag="IVW29_001408"
     CDS             complement(59..373)
                     /gene="trxA"
                     /locus_tag="IVW29_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7838897.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(460..2820)
                     /locus_tag="IVW29_001409"
     CDS             complement(460..2820)
                     /locus_tag="IVW29_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7838898.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(2935..3483)
                     /locus_tag="IVW29_001410"
     CDS             complement(2935..3483)
                     /locus_tag="IVW29_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7838899.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(3541..3978)
                     /gene="zapA"
                     /locus_tag="IVW29_001411"
     CDS             complement(3541..3978)
                     /gene="zapA"
                     /locus_tag="IVW29_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7838900.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4180..5097
                     /locus_tag="IVW29_001412"
     CDS             4180..5097
                     /locus_tag="IVW29_001412"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7838901.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(5475..6386)
                     /locus_tag="IVW29_001413"
     CDS             complement(5475..6386)
                     /locus_tag="IVW29_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7838902.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(6787..7365)
                     /locus_tag="IVW29_001414"
     CDS             complement(6787..7365)
                     /locus_tag="IVW29_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7838903.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7385..8053)
                     /locus_tag="IVW29_001415"
     CDS             complement(7385..8053)
                     /locus_tag="IVW29_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7838904.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(8068..9138)
                     /locus_tag="IVW29_001416"
     CDS             complement(8068..9138)
                     /locus_tag="IVW29_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838905.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(9309..10673)
                     /locus_tag="IVW29_001417"
     CDS             complement(9309..10673)
                     /locus_tag="IVW29_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7838906.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(10670..12229)
                     /locus_tag="IVW29_001418"
     CDS             complement(10670..12229)
                     /locus_tag="IVW29_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838907.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(12285..14765)
                     /locus_tag="IVW29_001419"
     CDS             complement(12285..14765)
                     /locus_tag="IVW29_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838908.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(14749..15084)
                     /locus_tag="IVW29_001420"
     CDS             complement(14749..15084)
                     /locus_tag="IVW29_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838909.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(15098..16132)
                     /locus_tag="IVW29_001421"
     CDS             complement(15098..16132)
                     /locus_tag="IVW29_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7838910.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(16222..16902)
                     /locus_tag="IVW29_001422"
     CDS             complement(16222..16902)
                     /locus_tag="IVW29_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7838911.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(17072..18031)
                     /locus_tag="IVW29_001423"
     CDS             complement(17072..18031)
                     /locus_tag="IVW29_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7838912.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(18252..19355)
                     /locus_tag="IVW29_001424"
     CDS             complement(18252..19355)
                     /locus_tag="IVW29_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7838913.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(19577..20047)
                     /locus_tag="IVW29_001425"
     CDS             complement(19577..20047)
                     /locus_tag="IVW29_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7838914.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            20304..>21294
                     /locus_tag="IVW29_001426"
     CDS             20304..>21294
                     /locus_tag="IVW29_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7838915.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYA"
BASE COUNT         6081 a         4450 c         3669 g         7094 t
ORIGIN      
        1 aaaaccgagg acggaactgg attcagtccc accctcagct tatttttgct gtaagttttt
       61 ataaatattg atcgatcatt tgacgtagtt gttcttttga atgaacacca acagcttttt
      121 ctactacttg accatctttt ttcaacatta atgttggaat gctcatgata ccgaattgtt
      181 gaggagtttc tggattttca tccacatcca ttttagcaat tctgaattct tcttcatcat
      241 attcttgttc caactgatcc aaaatcggtg cttgcatacg acatggacca caccaagttg
      301 cccaaaaatc aatcaataca agtcctttat ctgtttcttc attaaatgtc ttatctgtaa
      361 ttacttgtga catatcaaag acctccattt ttaaaatcta tgactagtat agcatttcta
      421 tacttggata gcgaaccatc tgcttacctt tagtaagctt tacttaaatt taacaattgt
      481 cgcaccattt ccgccttggt ttgccggtgc aaactcaaag cttttgacgc tccgatggtt
      541 tttaagataa tcagtaattc ccttgcgaag tgcgcctgtt ccttttccgt ggacaatagt
      601 cacttgagga taaccagcta gaatggcaga atccaaatat tgatctacct cacttagcgc
      661 ttcttcgtat cgttttcctc gaaggtccaa ttgattcggc acgtgactac tagattcaga
      721 tcgtacagca tggataactc gttgtgtcgg ctctttttga ggagcaacag gcgtcagctc
      781 gtcttcggac acattcattt ttaatatacc gatttctact tgccactgat tcccgttttt
      841 acgtaaaagc gtccctcttt ggccgtaagt cgtaaccaac acttcgtctc ccggtttcaa
      901 cgttttttgt tctttcgcct tcttcaatac tttatttttc ttcaaagttt cttcttgatg
      961 tagatttgct aattgggtct ttgcatcgat caactgatgc tctttcacat ttccttgccc
     1021 aatctgctgt tgcattttac gaatatcagc aatgattttt tctgcttttt cttccgcctc
     1081 tgacacgact tcatttgctt ttttctttgc tttttccatt tctttttcac gttcttcaaa
     1141 gaaatagctg taagcttctt tcaaatcatc gtgaagttct tgtgcctcag aaacaaaatg
     1201 acgcatttcc agatattcgg tctctgccat tttccggcga ttctctaaat ctgtgatcat
     1261 ttcgtttaaa tcttggctct catcattcat caactgcttc gcttcatcaa taacttctgt
     1321 atcaagacct aatcggctag agatttcaaa cgcattgctt cgtccaggaa cgccaatcag
     1381 caaacgatac gttggactta atgtatccac atcaaactcc atgctggcat taatcgtatt
     1441 tgcccgatta tatccataaa ctttcaattc aggataatgc gttgttgcca tcacataagc
     1501 agacttttta ccaagatcat ctaagattgc gattgctaga gcagcacctt cttgcggatc
     1561 tgttcctgca ccgagctcgt caaataatac cagacttttt tcattgactt ttgataaaat
     1621 atctacagtg ttcgtcatat gagaagaaaa ggtcgataga ctttgctcaa tcgactgttc
     1681 gtctcctata tccgcaaata cttcctcaaa aataccgatt tgactttctt cacctgcagg
     1741 aatcggtaat cctgcttgcc ccatcagttg taacagacct aaagtcttca acgtgatcgt
     1801 tttcccacct gtgttaggtc ctgtaatgac gatggcttga tagtcttttc cgattgtgat
     1861 gtcattagaa actacttttt cctgatcgat cagtggatgt cttgcttgtt tcaagacgac
     1921 atgattgtct tcactgattc ccggcacgat ggcttttact tctttaccaa atcgtgcttt
     1981 tgcattcatc agatccattt taccaatcac ataagtattg tggatgattt cttgacgatg
     2041 gggaacaagt tcagcagaaa gttcacttaa aatacgttga atctcatttc gttcagcgat
     2101 ttggtattga cgcaaacgat tattcaaatc caccacttgt tttggttcaa taaataaagt
     2161 ctgacctgaa gcactttgat cgtggactac accgccaaat acaccgcggt attcttgttt
     2221 gactgggata acgtagcggt catttcgcat ggtgatgatc gcatcactta aatactttgc
     2281 atttttccca cggacgatcc catctaactg ttcacgaaca gcttgttcac ttcgccgaat
     2341 gttctgtctt atgctcttta actcaggaga agcgtcatca gtcacgcgtc cgtcttcatc
     2401 gattgcttcc tttaaacggc ggctcaaaac agggatcgtg actaactgat ctgcccacat
     2461 atacaaacgt gcaaattcga tttcactgtc agaaagatca tcaatgaaac gattcaattc
     2521 gcttgtcgta gacaatactc tggaaacttg tgcaagctcc actccattca agtctgctcc
     2581 gatctcaatc cgtttcatat ggggacggat attttcaatt ttaggtatag ggatcccgcc
     2641 tcgcagacgt tgcactttca atccatcctc agtttcttga agccagtttg tgatcgtttg
     2701 tttctcactc actggtacca attctgccag ttcttctttt ccttgtgcag tcactacaaa
     2761 ttgtctgacc atctgtttga ctttttcaaa ttccaatgtt tcaaggattc ggttattcat
     2821 tttatcacct tatctttcac tatgaaaaaa gagctgtgcc ggacatacgc tacgcagcct
     2881 tcttaaataa actactctgt acaaaaaacg aaatattttc cagcactatt ttccttaatt
     2941 caaaatattc gttacccaaa gctcatacat cttgttggta agaaatggcg ttctctcaac
     3001 gataaaacga gcgaaactat gctcgccgaa ttgattttgg accacatcta atggaacaaa
     3061 ggcaagtaac tgcaaaatca aaaatatagt aacgtaagcc atgattagtg acaggatgcc
     3121 tcctgcaatc cagtctacct gtctcaagat cggcacatac gtcaatccat ggacaaaaac
     3181 ccccacaaat cgggtaagca gccagccaat tagcaaaata atcagaaaag cagttcctgc
     3241 ataaaaagct tcatctaggt gaaaggcaaa tacctgatca aaaaatgcta actttgatgt
     3301 tggggtgaca gctgggtatg gaatgtataa ttccaaatga cttgctaatg gtttgtaaaa
     3361 atgctgggca gcaataaatg acaatacata tccaatcgcg aatatgattt gcaaagcaaa
     3421 cccgcgacgt gctccagaaa aaaacgcaat tagcaataaa aataaaatca gcaaactcag
     3481 catgctacgt tcctttctct tctaactttt ttaaattttt cttattttga taaaaggatc
     3541 ttaattagaa gatttttgtt taatttgtcc ttttcgtttc tcgtttaaaa tctgttgtgc
     3601 ttccacatga tttttaatgg tatgatctgc ttgaccattt tctttcaata cttcccgagc
     3661 ttcgttctcg attgcttcaa tgcgtttcac acgattttca agctctgtaa atttgatcat
     3721 ttttttcttt aattctgctg tttcttcttc cagctccaat attctttctt gtttcttcaa
     3781 ttgatccgaa agcgcattga cagccatcaa gattgctgct tgttcattat ccatttgtgg
     3841 tgataattgt ttcaattctg ctaactgttc attgatcagc ttcgttacga tatccatatg
     3901 atgcttggtt tcccgtccga taatagtata tgtttggttg gcaatcaccg ctttgtagcg
     3961 tgttttttcg tgcgccatac gaatcatttc ctcctatttg atgaaagttt tatttagtgt
     4021 accatgaagt tggttctttc accgttctcc acaaatattt ttcacatact taactattat
     4081 atgataaact ttaaaaaaat gctatgtctc gcaatctctt agaaaacgaa aagtcgtgag
     4141 ttgcggtata gttttatgta taaaaacgag gagaatttta tgagcaacgt cattatcaaa
     4201 gcaacaaacg accaacttag tcaaatgaaa gcatactatc gctcttattt gttaaataaa
     4261 cagattcctt atactagctt tgctgcaaaa aaagacggga cgaccattac cgcttataca
     4321 tccggaaaag tgatgtttca aggtacgaat gcagcaatag aagctgataa atggggggag
     4381 gcacaggata aacaagccaa gccagcaaaa acagcctctt ctttgccaaa aaatatcagt
     4441 caattatccg ttttaggtag tgatgaggtc ggaaatggca gctatttcgg tccgctaaca
     4501 gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat tacgtaaact tggtgttaga
     4561 gattcaaaag agttaaaaga tagccaaatc atccagcttg ctgacatctt aaaaaaaacc
     4621 atcccctata aactgcttgt cttatctcca gaaaaataca atgagatcca accaaaatac
     4681 aatgccgttc ggatgaaagt cgctttgcac aatcaagcga tccatttgct gctaaaaaaa
     4741 atcgagccag aaaaaccaga agcgattttg atcgatcagt ttacgccgga agcaaatttt
     4801 aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa aactgtactt cgttactaaa
     4861 ggagaacaat atcatttagc tgttgctgct gcttctatca tcagtcgtgc cagctttttg
     4921 gaagagttag acaaagcatc agctgaagct ggaatcaccc ttccgtcagg agctggaacg
     4981 aaatctgacc aagtagcggc aaaactattg gagaaaggcg gcatgcctat gcttgaaaaa
     5041 tatgccaaac ttcattttgc caatacagaa aaagctttga agctactaaa aaaataatgg
     5101 aactgtgaca aaagtcatgt cacagtccct tgttccgaat aaacggtgtt caatcatctg
     5161 atcctcggta ttagctcaac aaacggcaaa atatgaggaa ctatttttcc gtttgtttct
     5221 ctaatactca gaacaaaacg ttgatttcac aacctttttc ttaaacggtg ttctcatgcc
     5281 aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt ttgaaattgc
     5341 tccttgtgac tcagagttga acggcaggct cacgacctct tccttaaacg gtgtttaaaa
     5401 gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta tttttgtttg
     5461 gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact tgcagcaatc
     5521 tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt catataaccc
     5581 atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt cccagtggag
     5641 attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg tgctaatgtt
     5701 tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc atattccgta
     5761 gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta gtcgcatata
     5821 tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc tccagcatcc
     5881 tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc ttctgtttgt
     5941 tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa tacaatcact
     6001 gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt tccatcttcg
     6061 agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt ttttgtttca
     6121 gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt tagcgagtca
     6181 tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg ttctgtatca
     6241 tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt ttgtccaact
     6301 atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat gattgcaaat
     6361 acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc agactaaggg
     6421 ccaaaactta aaatcagctt aagtaagcaa aaaaggggct gtaaaagata taaccgtaga
     6481 aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa atctcctttt
     6541 gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac aaacaattaa
     6601 tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat aaaaatatac
     6661 cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta tataaagaag
     6721 caagatataa cgatcttcgt catatcttgc ttcttcacct aactattttc tccctatata
     6781 acagctttac gcttctccta atacaccatt caaatataaa tcgatcaatt gcgccacatt
     6841 tagcggttct tccacaggtc ttttcgaccg agtgatgtaa ggtgctaaca ctaagaaaaa
     6901 atgccttgct gcgatttctt ttgaaatccc atttcttagc acagattggt ttgctgaaaa
     6961 gatttcttca aaaggttcac gataattttt cttccataga aagaaaagat gatctcttgt
     7021 ttcctttgaa agattgcttt caagatcgtg aagcatcaga aacaaatcga ccagatgtgt
     7081 ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga aatcccgttt cgcttgtttg
     7141 aacgatttct ctcaaagctt ttccaacatc ttttaagtgg ttttctaaaa ctgccgtata
     7201 tagtttttct ttatcaccga aataatgata gagattgggc tgggtgatcc caactttttg
     7261 tgcgatttct cttgttgaag tgccgagata tcccttggac ataaacaagt cagtcgcttc
     7321 tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg accatttttc cacctgcttt
     7381 ttggttattc tttttctttc aatacaccat cttccatcat aaatattcta tcgcatttgt
     7441 caatcagccg tgtatcgtga gtaaccatga tcgttgcttt atttttgtct tttgattctt
     7501 ttgccaagat atcgactact tctcgtgcct tttctgaatc caaactagca gttggttcat
     7561 ctgctagaat aatggacgga tcattgtaca atgcacgagc gattgcgacc ctttgccgtt
     7621 ctccaccaga tagttcttct ggatacttgt tgatcaactt atccacgcca agctgctcaa
     7681 ataattcgtt tgcttctttt tgacgatgtt catgtttaat cttatctact aataataact
     7741 gcttttttac tgtcagaaaa ggaaccagat tggaagcttg caaaataaag ccaatttctt
     7801 taaaacgtag tttaacgcgt tcgctttctt tttgattact gaattcttga tcattgataa
     7861 atacagagcc actgctaggc gtctgcaatc cgcctgccaa agtcaaaaaa gtacttttac
     7921 ctgaaccaga aggaccgatg attgcaacaa attccccttt atcgatactc atggttgttt
     7981 ctttcaatgc tttaactact tggtcgccat cttggaaact tttttctact tctttaaatt
     8041 ctattgcttt catgttcttc ctccttctta tccaattgcc ttcaacggat cgatttttac
     8101 gattgtccga acagaaaata gtgcgcctaa cacagctact actaacatca acaagctaat
     8161 gacgccaaaa aacagccaat tgctttggaa tggaaccgca tcaggtagaa caagggatgt
     8221 tcccactgtt cctaatagac caagaagaat accaacaaat gataaaatga atgtttgtgc
     8281 cactacagaa cgcgcaataa atcctcctgt gatcccttgt gctttcatta caccaaagat
     8341 attaatcttt tgcatcgtca gtacataaat gaaaatcccg atcacaatag ccgcaataac
     8401 gatcaaaaat ccgatcataa atccaaaagt caatacttgc gcattatatc caggtaattc
     8461 attgatgaat ttagaaattt tgatctgttc taaatcttca ggtaaattac tgattttacc
     8521 tctgacaact attgcgttaa tccgtgcatt ttcacttgta tcttcttttt cgaagcggat
     8581 ttcttggtaa gcattgatgg ttgtatagag aactggtgag acattgaatt tggcatgatc
     8641 cgtaaagcca gttagtttaa acgtcttgtc actaccagat aacttaactg tatctcctat
     8701 ggctagacca tattcttctt tcaaactaat atcgcctact gcctcatcat cgttatcaaa
     8761 agcttttcct tcaacaagat tgggcatgat gaattgattt ttatcgatac cgaagaagct
     8821 aacattgatt tttccttctt ctgtactgtc tttactagtc acgactccag gtgtttgggc
     8881 aagatacgcc actttcccac cttcgacttc ttctgctatc ttcttagtaa tcatggacat
     8941 ccctagattt gaatttgcat ctttcgaaag cacaatcgaa tctgcctccc acttgtcgac
     9001 tgctgttcga ttatcttgag ctaagccata agctaaccca gtcaaaaaaa ataccaggta
     9061 ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt aactttgaat gcataatttc
     9121 atttaatgct aaaaacattt aactcctcct cctgaaataa tcattttata caacaatttt
     9181 atcacttgat aaatttttgg cgaatattat gctcaaaaaa ttcttccgct atttcattat
     9241 atgaattttt tattctcgat tgattaatat ttattatatc cccacacaat aaaagaccgt
     9301 gatatttctc acagtctttt atctactcta ttgctatttg ctctcttagc tagtacttta
     9361 aaagctaact cattgaggta gtgaaaacga gaggtgtatt cagacagaaa tccaaaatcc
     9421 tcaactgcta gctgtggaat cgtagttttg tgtttcacga tatccgcaag caggttcaaa
     9481 tctgataata agaattcttg gcttatcagc tgtccgccaa ttatttctaa tgtatccgga
     9541 tggtagatca gctttgcatt tactgttttg ttttcttcgt cgtattgtgg aaatagatca
     9601 aattctcctg aacacgaacc ggtattttgt tccaagaatg gcgcttcatc ttctgtcagc
     9661 cctacagttc ctaaaaagta gtctataatg actgctgctg ttatcttttg tctgaggtcg
     9721 taagcggttg gctgaccact caaagttagc gctactgctc ttgccatatg tacagctcga
     9781 gtaactaaag gtaaaaagag atgccgttta tttttgtcaa aactgactgg gacgatatct
     9841 ccaatcgcat agatgtccgg atcacttgtc tgcagatatt cattgactat cacagtctca
     9901 tcctcataca aagccacttt ttcttttaac aatgtcgtat taggacgagt ttgggcagaa
     9961 aaaatgacca tatctgcctt tattgtttga gacagaagtt gtacggatac tactttctct
    10021 tctgattttt tccaatcgat caagaattca tttggataaa aatttatttg cttagtttgc
    10081 agctgatttt gaaaactgtt gatcatatcc ttatcgaaat atcttgataa aacattcggc
    10141 atataatcaa ctagatgaat aatgtatcct ttatttctca aagcatctgc taattccaat
    10201 ccaatgtaac cagcaccgaa gatcactact tcttttgctt cttttaaagc ggcaagtact
    10261 tgcttagatt gcactagatt tttataggtg tagacatttt cagcttcaat ttgtttatac
    10321 aaggtgacag gagaggaacc tgtcgctacg attaatttat cataggttat ggtctcgttt
    10381 tttccttctt tggatgcgag gatgagctga tgtttgtctg gatcaagttc agttactctc
    10441 cattcggtga tcagttttgc cccactttct tgaagagtcc gcatgttatg ggcaacttca
    10501 gctaaactac gtatctttcc ttttaaataa aggttgattc catttgaaac atagcctagt
    10561 ccatctttat gatcttcatc gatcagtaaa acttctgctt tcggttgaag ttttcttaga
    10621 tttagggcag ctgtgatgcc ttcatgggaa gcaccaatga tcacgatttt catgttttat
    10681 tcatccttaa attcaatttt tctaaataac attcttttaa aaattggttc aaatagggga
    10741 aatcataatt atattcgttg ctagtgaata caaaaacttt agcggacttc tgtgacgggt
    10801 actctttcaa ttggctagtc aaaatgatat catactgctt ttctttctcg tattcgacta
    10861 aatcatattt gatcgtattc agcaattgtt cctggatgat ttctttcacg atatccgcca
    10921 ttagcctttc ataaggaaga tcataagcaa tttgcagacg taattctaac tgcttgatca
    10981 gcgtccgtaa aatagatgca tagcgagcaa ataattcaaa gcgggcattt tcctcgtcta
    11041 cttcagtaaa atcaacaata gcaaatgttt gtctgaccaa tctttcacat aaaaccagga
    11101 attgtttatc ttctacttca gttaaccgct gttttaatcc ttcttggcca aaaatattca
    11161 tccatcctct aaaataggca aaatgagcgt gattttgcat cactgtcaac gcaatctttt
    11221 gcgaaatctc ttctggaaaa acataaaaag agaaatattc ttctatcgat tgtttaaacg
    11281 ttcgattcaa ttgagctact acgtcgtctt tgtatttttc gcttatcaaa aactcctgga
    11341 catgcgagtt atgtgtatct aatgtaaagt tactgatcat gaaaagataa aaaaccaacg
    11401 tttcattttg attccaagga aaggcatatt gaaagaagta acgggtcaaa aatgtatgga
    11461 ggtccaataa aaattgtgtc gtttgaaaca atccaacagg aagattgcta taatctggtt
    11521 tggatttttt cgtacagcgc attcgcttct tcataatgga aagccatatt tcaaatttca
    11581 atcgtccttc ttctgtaata gcaaaactaa actctttttg cagcagatct aaaaaagtgt
    11641 ttcctgataa atcactgtat tcttttttgt tttcaagata gggacgtcca aaccaaaata
    11701 attggaaata aaaataacga atttgacttt ctggaccggt cagccgcccg ctttttattt
    11761 tcagatgaaa ctcatttaaa gaatcattta attccttcac tctgcgagac aaagtagccg
    11821 aactgatatt aagttccatg acaagtttct gccgatcaaa tgagccattt tccaacagat
    11881 aaagaagtat ctgatatttt gtagaatagc gcaagtaaca atgataataa acatcgactg
    11941 ctgctatatt tccatgtctg attaataaaa tattcccttc ttggataaca atttccaatt
    12001 gtttgatatt ttccaagtta gcttgtaaat aaaacagatc ttctttcaag gtagcccgat
    12061 caactgataa gcgggtaagt acgttattaa cggaaacctc tcctttttcc tctaataaaa
    12121 gttggaaaag tttgatttgc cgttgttcat ttttttcaag caaactgaaa agttccaagt
    12181 ccactccttc aagcatttct gattttaaca gcagaagttt ccttctcatc cttgaaaaag
    12241 aggatctgtg acagttatca cagatccata caaattctaa tcacttagct ggttggccca
    12301 gctgtcagtt cccatgtgac catgccttga tacgttgcgc caggttgagc attcgtactg
    12361 ctatttacct ctaagagtaa accatcggta tcgggaaagt caacgagaac ctctccttta
    12421 gctgttccat cactctcata aatcaacgtg ttttgacctt gctgaatcaa ttgctttgat
    12481 ccagaatgat tgaagtataa ttttgttccg cttaattctt gaccttgatc attcttaaat
    12541 aaagaagtca atgtacctgt cagttgccaa ggagacgtgc ttggaagccg agtatcccgc
    12601 acatcaaaag aaaaatcgcc ttttgattca taataacttg tcttcaacgg tttggggaga
    12661 tcgttaccaa atgtaaaatc atctggtaca ttaactaatg ttaattcttg tttggggaca
    12721 agaattgttt tagcagatgt ttggacaact gggttaggaa gcttagcacc atctgctaat
    12781 gtaatattgc tggaaagtac agctgccttt gtcagtatgc tgacggattg taaagaagta
    12841 tctacagtat ttaattgata gttgtaagta agatgaatgg tatctcctat tttgctcaat
    12901 agatctgctg ggacagaaac tccttgttga atgccggtat ctgcgatagt tgtaccattt
    12961 aattttaagg aaccattgat ataagtagta ttttcaggaa gtgagatcgt gatttttttg
    13021 tcggttatcg catccccaga ttttgttaac gtgaatgtca cattttcttg aatcgtatct
    13081 cctgatagag cagacagggg atctcctgtc tcaggttgtc tgctaaggtt ttttagctga
    13141 ctatctgcac tcacagatgg aaaattagcc actgttattt gaggaagagt ggtgattcca
    13201 gcttgttcag actgccattt gtacgcaaaa gacggataca tgttagtaag tacatcgtcc
    13261 accaagcgat aatcaaaata atattgccct gaagccaatg caggtagtgt agaagtagct
    13321 gtatttacac ccccgttatt tgattggatc gtttggaaaa gttgatctgt ccatcctgtc
    13381 gctaattttt gggttccttt cgaataaagc ctgtaatgga tcgaaccctt ttttagttca
    13441 gttgctgacc aattggccgt gacttggtac gatgcagaag aaaggagctt tccccactga
    13501 ctatcggctg ttaagtttgt tggtaaaacc gttccgtcta gcttgtactg ctccaatacc
    13561 tttaaagcaa caggagaaag caatccgcct tgcgcatctt tcccactgta cacgatattg
    13621 gctgtctgat tcaatccact tgtgcttgct ggagtatgag tcagtattgc tccttgtgta
    13681 acgttttttc ctgtaaaata gttgactacg cgatcaaagc gactaaatgg caactgatca
    13741 aaacgtcctt cagtcagccc catcgtcaat gtccgactat tggtagagcc tggaaggttc
    13801 tggttatttc catccacgta ttctgtggta tcagcaaatg tattatattt tcctgttgaa
    13861 tcacttggtg agatgtaact attccacgta tttatctctg cacttgcaga aaatgcatat
    13921 gctcctggat tcaagtttcc gccagttaat ttaggataaa atctacctgc agctggagct
    13981 aaaatcgaat tgaacatacg atcttggata atattattcg aatcataaaa atcaacagaa
    14041 aaaacggcct ttttagacat agaaggttgt aatttaccct tggtcaaaat tttgtttgga
    14101 ctcgtatcat atttatctcc gttttctgtc atcttcgcga cattagctgt aacatcaaat
    14161 tgaagcggga tatcgtccac gttgaattcg cctcgcgtaa taatagcaat aatatctgtc
    14221 aatagagtat ttgtatccca cgaccccatc ccaaaacgca aggtatggct tcctgctgat
    14281 aataaacttc cgctagtaac cttgatgggg gcaccggcaa tatacaagta ggcgttgttc
    14341 cagtcaattg cttttaatac atcactgctt gatacatgtt ccggcaaatt gatgatcgcc
    14401 acaaatgcaa atccttgtgc agtgatattc accaaatcaa gtacatttgt tagtttcaca
    14461 tggctttccc aatgaaaacg gaccgtgcca cctttccaag gatcatttga accatcgttc
    14521 aaataagtag cagtcaattt aggcaattgg ttagccccag ttagttggac aagcttcccg
    14581 ccattgactg cactacgtcc accatttgga ggtacgatat tttcatacgt gtatggatca
    14641 taagaaatgg caggaggcgt tgctccaaca gcttctgccg aaggtgaaaa gataaatata
    14701 cttaaaacaa aaagcgaagt aaaaaagaag gagcgaaaaa atgaaaattt attttgtagc
    14761 aacatgttca tcctcctttt cctttcttct ccataacaac aaccacgcaa gaagccataa
    14821 ttctatacca atgatcaaat aaagaaaacg gatcttttgt tcattcgtct ccgggaatac
    14881 gccatgttga ttagattctt tataaatcgg tagctgtgtc tgctgttttg atgcgccatt
    14941 gactgaacca cttggatgcg gaattggact tagatcataa aatgtaatcg tacctgaact
    15001 tttggtatct gcttggactg gctcttgcca gctgaagaac agcaaacttg caataatccc
    15061 tcccaataag atcgatgttt tcaacgaaat cacctcatca tgttgtttct ccttttttct
    15121 ttttcttgtg gacccacaaa aacagcaaaa tcaaccataa cactagaact gcagcaacca
    15181 atcctgcata aagccatggg aaagattgtt cttgtactgc aaaaacggaa ttttcattta
    15241 ctttttgtgc tttttctttt ttgatcgtaa agttttgtgt aaatcgccat tcattactgt
    15301 cgcctgattt tgctatgatt tctgcgcggt aatcgccggc ttttgcttta tcaccagcta
    15361 aatcaagatt caagtgaaaa ttcgaatttg gtgcaactaa aaatgcatta gacgtatttt
    15421 caatataaag ctgattttca taaaagatct tgctcgtaaa tatcaaattg ctgataatcc
    15481 taggctgagg attttggaaa cgaagctgaa cagttggctt tgcgttgacc tcgtccaaat
    15541 ctcctgataa aagtttcaga tccggttcta tcgtctcttg actttcttgt aaaacgactg
    15601 cgagattata agcaatacga tttttgattt gtgctccttc tgtagcattt tctgtttcag
    15661 cttcttcttt aggtgaaaca tgaatacccg ctaatacacg tcccttaaag ctttttgctg
    15721 ggattttcac ttctataggt acagttgttt ctgcatgtgc tgatacattg atttcctttt
    15781 tcgcaatgag caaaacgtca cggacatcaa acggcaatgt ctcatctaac gttgggtttt
    15841 tttgactata atcgatcgtt ccaccatcac tcgttaccgc cggattgaca gagacctcaa
    15901 atgttcgttc ttcatctgaa gaattgctga taattacttc tagtgtctga ttttgttccg
    15961 gtgccactaa taaatcaaaa taaccgattg ctttatctat ttggttctct ggaaataccg
    16021 gtttgattcc aaagtctcct gctgaatcct ttgcttggct gattgacgga aaaatgaaaa
    16081 atagacttaa aatagaaaaa aacgaaacaa acagtcgttt actttgattc attgtccacc
    16141 acatcctttc aacaacaact gcggtaaaaa atccttcacc tatagtattt tcaccgcagt
    16201 ttgttgaatt tatttcatac tctatgctac tgggccagcc acgaggttcc aagtcaaatc
    16261 agccgtgaat gttccagctg ctgctttcgg cactttcagt gtgatatttg ctggatcata
    16321 aacagaaagc catgttccca gaccttgatc ttttgctgct gtaaatattg gtgtcgctgc
    16381 acttgcttta ctcaacccag ttacagtttg actagttggt ccagtggatt cgttatctga
    16441 cgtactttta acagtggatg ttccaaaatc taattcagcg ccttgcaaaa cttgtgaacc
    16501 gtttacaaaa tctgaaaccg aaactgtcac gttccaccct tgtgcttggc catctgcacc
    16561 tacacctcga cgatctgata cttggagata aggtgtatcg ttttttgaag cgtctacttg
    16621 atacgttttt gttccgcttt caatctcgtg tgtaccaaat ggcaattcgg gaactacatc
    16681 cagtgttaaa ggacctgtct ggccagttgc tggattttcc ggatctgctg gctctactgg
    16741 ttttgaagga tcagtagggt taagaggctc cacggtattt gttggttcag taaaggtaat
    16801 atctcctttt gaagtcgctt gaccctcagc aaaagctggt gttgccaagc cgcctagaag
    16861 aagtgttgaa agtaataatg tcccaatcat gttctttttc ataataaatc actccttttt
    16921 cttagtgacc taagtatagt agagacagta tgcaaaaaat cctcatgaat tcatccgctt
    16981 acttgaaatt atgacccttt ttgtataaaa gagaaaaaaa caataaaatc gtttacatga
    17041 atattcttcc ttttttatta agtttcgttt gtcaaattaa gttagacaaa tcatcttttg
    17101 tgacgtaact caaaaatact tccattggtg tccgatagtg taatgatttt ctaggaatat
    17161 gatttcgttt cgatgccacc gattggataa aggtttcaga tacaccatta aaatccatag
    17221 ctttatacaa accatcttta cgtaataagc catttgaatg ttcattcaat cctcgttgag
    17281 aaggtgttcc tggatccgca aaataaattg aaatatcgtt ggtattacta atctttttcc
    17341 aatttgagaa ttcttttcca caatcaaatg tgatggattt aaataagttt tttgggacac
    17401 aacgaaacca ttgattcagc ctattttcaa tgtctttagc ctgtctgcct tcgggtttca
    17461 aggtaatgat gactttagat agccgttcaa ctaatgtgat gaccgcgctt ttatgttttc
    17521 cacccacgat agtgtcacct tccaggtgac caaattcatg atcaaactgg gtatagtctg
    17581 tctttctgtc tcggattgac cggcgaaacc cttgttttcc tcttttttct ttatgaccgt
    17641 ttggttttct ttttcccttc attggtaaat cgtgagtgtc aaaggtttta tctttaaaca
    17701 ttcgatacag tgtgcgtacc gaacaagcaa taggaaactc tgctcgacca ataaggacat
    17761 caggagccca cccttgaaca accttttttt gaatgtattc ctgttgctca tcaggtaaaa
    17821 caatcggatg tcttccgcag ttacgtttgt ttttcttata ttgtttatag tagctcattg
    17881 cgcttccacc agatttaaga aaacgataga ccttataaat agtttgtcga gaacgttgta
    17941 acagattggc tacttttgaa acaggttgtg attgattgaa ataagcttct atcattacga
    18001 gttcagttgg tgtaagatgg ttataggtca tttgtactca ctccttataa ttctttcgtc
    18061 ggaactattt tgagtgtagc acaaatgact ttttttagtt gtctagctta attttacaat
    18121 cggcgaagta tgaaaaaata tttcaatttt attttttcgt ttcaaattat tctattagtg
    18181 acatccttga actataaaaa taggagtacg gcatcatctg ccgcactcct atttttttcg
    18241 tcatttgttt cttaaataaa acgattctca cgcaatttta tttttttgat atcttctact
    18301 gtttgtgttc cagctaattg catgaccatt tccaattctg ttttaaagaa atcaaacact
    18361 tgatggactc ctgtacttcc tcctaaagac aatccataaa taacgggtct gccaatagca
    18421 acaagatctg cacctgatgc gattgctttg aaaacatgct gtccgcgtcg tacaccgctg
    18481 tcaaacacga ttggtacacg tttatctact gcttctgcca catattgcaa cgaatcaaac
    18541 gctgctggac caccatctag ttgacggcca ccatgattag atacccagat tccgccagca
    18601 ccagattcta atgaacgata gacatcttct tctgactgga cacctttcac atagactggc
    18661 aaatcagaat gagcagcaat aaattctaca tcttttggac taagcttctg tttggaagat
    18721 ttgtacacag catccatcgt ttggcctact cctgattgat aagcctgaac gatcggcatt
    18781 ggcagtggga aggtaaaccc atttctgcgg tctgtttcac ggtttccgcc aactgttgca
    18841 tccgctgtca atacgattgc ttttgctcca ttacgcttgg ccacttcaag aatatccaaa
    18901 ttgatcccat catctttgct catataaaat tgaaaccatt gcggtgcttt ctccccgcct
    18961 gcttctctta tctcttctaa tgtacaagaa gcgtaagaac ttgctgtata gatcgtccca
    19021 aaatctgcta caccttttgc agaagctttc tccgccttca catgagctaa gccatgagca
    19081 gcaactggtg ccatgatgat tggtgcagtc agcgtttctt tgtcaaaatg tgtagttgta
    19141 tcgggtaatt ctacatctct taatacatga ggaataatca gctgatgatt gaatgcccgt
    19201 tcattttctt gataagtaaa taagtcgcca gcaccactgc tgatgtaacc ataaccacca
    19261 gctggaatca cttgtgaagc ggctatttct aaatcttcca tattgataaa atccactatt
    19321 ccttcatttg tacctgcttg atacgttttt tccatttcaa tgccttccct ctataaaaaa
    19381 ttgaatgaat aatatgatag cgtttacaca ctttatatta ctcttccgta ttgatacagg
    19441 caaataatct gtttacattt cagtcattcc agttgttact actctgaaag aaatgtttag
    19501 aacacaaata gataaaaaac gatgggattt tgacaaaagt cgtgtcaaag tcccttttac
    19561 gaaatactgt ggatgcctat tgaaataagt gcgggttgtt tctttgtatt tcaatcgttt
    19621 cttgccattc ttcgtttaat tttggattac cgttttcttc cttgaacgca taaggtccca
    19681 atccaaaaag ctcagttagt atttgattca cgattccata tagctcggtg tgatcggaca
    19741 catgattatt gaagatcatc tcattatcac cacgtttgaa tctcacattc gggctctcat
    19801 gatgaacctc tatgcagttt tccccttttt ttaactgacg gatttctgaa aacatctcat
    19861 aaatatcttc tccaataaaa ccttcagcaa gcgccagact gtccacaaga ccgcctactg
    19921 tcaactcaga gattatcgct ttgttggcac caagctttaa tgtatagctt gttgcttcta
    19981 ctacgccgca tgcattgcaa ccaccttctg atagttcgct ttttctgatc aattgaactt
    20041 ctttcatttt ctgttcctcc tcttagtttt ctacttatgt attttttatc acctgacgta
    20101 agtgtatttt atcgtcacga tcacgtgatt tattcttcag agattctacc tgaataattc
    20161 atacttttaa ttaaaaccat gtgaaactgc ctcacggcag cttacaatta ctttttcatc
    20221 ttcacccgtt aagtgatgaa aaaagaaccc ctttctgcta tggttaaagt gactaaacca
    20281 accaaaagaa aggagttctt ttcatgacta gcttacacaa aaaccaagta aaattcaatt
    20341 caaatatcac tatttctcat acaggtggtc aattatcgag tgattcgggt ctcgtcctag
    20401 tgaaagagct gatgaacatc tttggttttt ctgaactggc taagcaacac attcacattg
    20461 aagacgaacg agcgtatttt actcatgaca acttatccat acttgagcag ttcattatgc
    20521 aattaattgc tggttactca gctgattctg cagctaatct cctgagacaa gatcctgtgt
    20581 ttaaagctgt tttagatagg aaagaactcg cttctcaatc ttcactttcc cgatttttag
    20641 atcgactatc tgaggagaat atccatgaac ttcaagcttt gaaccaagaa cttatcgata
    20701 aagcacgcct catccgtaat gatacggaat taatcattga tttagattcg acccattcag
    20761 atacatttgg tcaccaagaa caaacgaatt ataatacaca ctaccaaacc tatggttacc
    20821 atccattggt agcttttgac ggattgactg gtgacttctt aaaagctgaa ctacgttcag
    20881 gaaatcaata tacatcaaaa ggcgtaaagg agtttctcac acctttatta gagcactata
    20941 atcactctct accaaacact gacatcttgg ttcgtggaga cagcgggttc gctacacctg
    21001 gtgtgtatga ttcgtgtgaa tcaaaaaaga gtcattatgt tattcgactg aagaataatc
    21061 gtagactagg tcaaatagct gagaaatcag tactttatgg cgataatcaa aagtgggaag
    21121 aacgagaagt tcagtacttc tcgaccactt atcaagcaca atcctggtca caaagtcgtc
    21181 gcgtgtgtat acgctcaaca cgtgaagcgg gcgaactact ctttcgacat gaatttatcg
    21241 tgacgaatct atcagaaaat gtttctcctg ataccatctt ttctatctat gcca
//
LOCUS       DACCVU010000045        20996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCVU010000045 DACCVU010000000
VERSION     DACCVU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.045"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(6..335)
                     /locus_tag="IVW29_001427"
     CDS             complement(6..335)
                     /locus_tag="IVW29_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7838916.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="IVW29_001428"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="IVW29_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7838917.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="IVW29_001429"
     CDS             1256..2269
                     /locus_tag="IVW29_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7838918.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="IVW29_001430"
     CDS             2269..3546
                     /locus_tag="IVW29_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7838919.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="IVW29_001431"
     CDS             3609..6311
                     /locus_tag="IVW29_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7838920.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="IVW29_001432"
     CDS             6463..6780
                     /locus_tag="IVW29_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7838921.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="IVW29_001433"
     CDS             6810..7130
                     /locus_tag="IVW29_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7838922.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="IVW29_001434"
     CDS             7238..8698
                     /locus_tag="IVW29_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7838923.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="IVW29_001435"
     CDS             8766..8987
                     /locus_tag="IVW29_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838924.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="IVW29_001436"
     CDS             9018..9200
                     /locus_tag="IVW29_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7838925.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="IVW29_001437"
     CDS             9200..9613
                     /locus_tag="IVW29_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7838926.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="IVW29_001438"
     CDS             9736..10917
                     /locus_tag="IVW29_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838927.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            11306..11379
                     /locus_tag="IVW29_001439"
     tRNA            11306..11379
                     /locus_tag="IVW29_001439"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="IVW29_001440"
     CDS             complement(11448..12587)
                     /locus_tag="IVW29_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7838928.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12886..13521)
                     /locus_tag="IVW29_001441"
     CDS             complement(12886..13521)
                     /locus_tag="IVW29_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838929.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13634..14269)
                     /locus_tag="IVW29_001442"
     CDS             complement(13634..14269)
                     /locus_tag="IVW29_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7838930.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14303..14764)
                     /locus_tag="IVW29_001443"
     CDS             complement(14303..14764)
                     /locus_tag="IVW29_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7838931.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14894..15325)
                     /locus_tag="IVW29_001444"
     CDS             complement(14894..15325)
                     /locus_tag="IVW29_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7838932.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15343..15663)
                     /locus_tag="IVW29_001445"
     CDS             complement(15343..15663)
                     /locus_tag="IVW29_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7838933.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15962..16738
                     /locus_tag="IVW29_001446"
     CDS             15962..16738
                     /locus_tag="IVW29_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7838934.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            16753..16956
                     /locus_tag="IVW29_001447"
     CDS             16753..16956
                     /locus_tag="IVW29_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838935.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            16972..17310
                     /locus_tag="IVW29_001448"
     CDS             16972..17310
                     /locus_tag="IVW29_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7838936.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            17297..17476
                     /locus_tag="IVW29_001449"
     CDS             17297..17476
                     /locus_tag="IVW29_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838937.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17519..17989)
                     /locus_tag="IVW29_001450"
     CDS             complement(17519..17989)
                     /locus_tag="IVW29_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838938.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18076..18774
                     /locus_tag="IVW29_001451"
     CDS             18076..18774
                     /locus_tag="IVW29_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7838939.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18952..19293
                     /locus_tag="IVW29_001452"
     CDS             18952..19293
                     /locus_tag="IVW29_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838940.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19286..19957
                     /locus_tag="IVW29_001453"
     CDS             19286..19957
                     /locus_tag="IVW29_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7838941.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19963..20649
                     /locus_tag="IVW29_001454"
     CDS             19963..20649
                     /locus_tag="IVW29_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838942.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            20652..>20996
                     /locus_tag="IVW29_001455"
     CDS             20652..>20996
                     /locus_tag="IVW29_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7838943.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNT"
BASE COUNT         6998 a         3457 c         4076 g         6465 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt ctaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    12241 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    12601 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    12661 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    12721 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    12781 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    12841 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    12901 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    12961 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    13021 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    13081 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    13141 tacaagaaga taatactcct cgttatccca tgaagcgctg ttaattcgaa gtaaacaatt
    13201 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    13261 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    13321 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    13381 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    13441 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    13501 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    13561 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    13621 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    13681 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    13741 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    13801 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    13861 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    13921 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    13981 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    14041 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    14101 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    14161 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    14221 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
    14281 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
    14341 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
    14401 ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc tctatatatt
    14461 tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg ttttcatctt
    14521 tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta aattgtatat
    14581 aaccattact catacctggt ttttttaatt gtacggctga tatattttta aaggggatag
    14641 ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta ataattgaaa
    14701 tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa atctcttttt
    14761 ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca tatatgagga
    14821 aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    14881 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    14941 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt
    15001 tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
    15061 ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat
    15121 cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca tgagataaat
    15181 gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt ctgggttttg
    15241 gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg tattcgctca
    15301 acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat tttcctctaa
    15361 agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
    15421 agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc tttcttctct
    15481 acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta attcatctga
    15541 attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa aattaatttc
    15601 taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt cataggtatt
    15661 catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat acgcagaaat
    15721 aatttgacta attctgagta atgagctata ctatatcttg taaacagatt taacaactaa
    15781 aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag ctttaaaatc
    15841 aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca gatactgtca
    15901 attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag gaatgagaaa
    15961 tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa
    16021 tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa
    16081 agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa tcacgacctc
    16141 aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt taatcttaaa
    16201 atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag tgttgccagc
    16261 aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt tgcttaatcc
    16321 agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa gattaatcgc
    16381 agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca tattatcttc
    16441 tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc cacaacagat
    16501 gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat ggttattgtg
    16561 caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc cgaaagccga
    16621 tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc tatttatcta
    16681 cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa ttggttagaa
    16741 aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga aaaaatcaaa
    16801 gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag atttggaatt
    16861 agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga agcaaacaga
    16921 attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat
    16981 ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt ggattatgag
    17041 gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt gctagaacgg
    17101 attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag atatagaaat
    17161 attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga aggaagagac
    17221 acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt ttcggaaatc
    17281 ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg caccgctaat
    17341 catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga ttgtttacat
    17401 ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga aaaagccaac
    17461 cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt actattactc
    17521 agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata tcaacaattt
    17581 tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta aggagtttat
    17641 taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa
    17701 tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct cctttagcaa
    17761 ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat gaaagttcta
    17821 aattaactaa catagctggt tctgttgaat agttagaaat gattactttt gtataaatca
    17881 tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc attcctttta
    17941 tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc cattgaaaaa
    18001 ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat tataccaaaa
    18061 aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc tgtgactacg
    18121 agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag agatattgat
    18181 gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa tgaaccagat
    18241 tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt cttgctggct
    18301 atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga agcattcaac
    18361 gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga tatgatgatc
    18421 tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg
    18481 cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggga
    18541 gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa
    18601 ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag
    18661 agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc gatccgtatg
    18721 gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga atagagctga
    18781 agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata aaccaatcat
    18841 cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa agtgactccg
    18901 ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca tatggaaaaa
    18961 gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc gaaaattgag
    19021 gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact agcttgggat
    19081 caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat ccatctatcg
    19141 aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat tgatgcgcta
    19201 gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg taagaaatac
    19261 attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga actttgaaaa
    19321 actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg attatttatc
    19381 ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg taactattaa
    19441 tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg ttgaggaata
    19501 taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa cggttaaagg
    19561 acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag ttgttaaacc
    19621 aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat tggctctaca
    19681 cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta gaatcgatac
    19741 aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga tgggtaaaga
    19801 tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag ggctcttagc
    19861 tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga tggagcgagc
    19921 aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa accattaatc
    19981 gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc aaccatcaat
    20041 gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg tgatcgagtc
    20101 attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa cttcatctat
    20161 gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg gaaggaattc
    20221 ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga cgaatcgaat
    20281 acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat ctttgaacat
    20341 catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata ttacttctac
    20401 aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga catcgatcac
    20461 tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga gtacacattt
    20521 gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat taatttcaaa
    20581 aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt
    20641 ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc aacgtaaggt
    20701 acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg gccttgtgta
    20761 atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat ggtgtgaata
    20821 aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg aatcgggaaa
    20881 tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt aatcaatacc
    20941 ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat aataca
//
LOCUS       DACCVU010000046        20180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCVU010000046 DACCVU010000000
VERSION     DACCVU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.046"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="IVW29_001456"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="IVW29_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7838944.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="IVW29_001457"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="IVW29_001457"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7838945.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="IVW29_001458"
     CDS             1947..2645
                     /locus_tag="IVW29_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7838946.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="IVW29_001459"
     CDS             complement(2813..5452)
                     /locus_tag="IVW29_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7838947.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHDIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="IVW29_001460"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="IVW29_001460"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7838948.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="IVW29_001461"
     CDS             7295..8470
                     /locus_tag="IVW29_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7838949.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="IVW29_001462"
     CDS             8474..9115
                     /locus_tag="IVW29_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838950.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="IVW29_001463"
     CDS             9112..10029
                     /locus_tag="IVW29_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7838951.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="IVW29_001464"
     CDS             10026..10697
                     /locus_tag="IVW29_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7838952.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="IVW29_001465"
     CDS             complement(11238..11726)
                     /locus_tag="IVW29_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7838953.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="IVW29_001466"
     CDS             complement(11927..12193)
                     /locus_tag="IVW29_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7838954.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="IVW29_001467"
     CDS             complement(12193..12456)
                     /locus_tag="IVW29_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7838955.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12449..12721)
                     /locus_tag="IVW29_001468"
     CDS             complement(12449..12721)
                     /locus_tag="IVW29_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838956.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12932..13117
                     /locus_tag="IVW29_001469"
     CDS             12932..13117
                     /locus_tag="IVW29_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7838957.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="IVW29_001470"
     CDS             complement(13259..15082)
                     /locus_tag="IVW29_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7838958.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="IVW29_001471"
     CDS             complement(15680..18385)
                     /locus_tag="IVW29_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7838959.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="IVW29_001472"
     CDS             complement(18691..19638)
                     /locus_tag="IVW29_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7838960.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="IVW29_001473"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="IVW29_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7838961.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         5753 a         4271 c         3776 g         6380 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgtcgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag
//
LOCUS       DACCVU010000047        19648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCVU010000047 DACCVU010000000
VERSION     DACCVU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.047"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..669
                     /locus_tag="IVW29_001474"
     CDS             22..669
                     /locus_tag="IVW29_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7838962.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            753..1697
                     /locus_tag="IVW29_001475"
     CDS             753..1697
                     /locus_tag="IVW29_001475"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7838963.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1832..3283
                     /locus_tag="IVW29_001476"
     CDS             1832..3283
                     /locus_tag="IVW29_001476"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7838964.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3304..4140
                     /locus_tag="IVW29_001477"
     CDS             3304..4140
                     /locus_tag="IVW29_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7838965.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4140..4799
                     /locus_tag="IVW29_001478"
     CDS             4140..4799
                     /locus_tag="IVW29_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7838966.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4925..5614
                     /locus_tag="IVW29_001479"
     CDS             4925..5614
                     /locus_tag="IVW29_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838967.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5620..6576
                     /locus_tag="IVW29_001480"
     CDS             5620..6576
                     /locus_tag="IVW29_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7838968.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6620..7132
                     /locus_tag="IVW29_001481"
     CDS             6620..7132
                     /locus_tag="IVW29_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7838969.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7388..7936)
                     /locus_tag="IVW29_001482"
     CDS             complement(7388..7936)
                     /locus_tag="IVW29_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7838970.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8025..9206)
                     /locus_tag="IVW29_001483"
     CDS             complement(8025..9206)
                     /locus_tag="IVW29_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7838971.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9442..10950)
                     /locus_tag="IVW29_001484"
     CDS             complement(9442..10950)
                     /locus_tag="IVW29_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7838972.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11109..11300
                     /locus_tag="IVW29_001485"
     CDS             11109..11300
                     /locus_tag="IVW29_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838973.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11503..12420
                     /locus_tag="IVW29_001486"
     CDS             11503..12420
                     /locus_tag="IVW29_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7838974.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12413..13486
                     /locus_tag="IVW29_001487"
     CDS             12413..13486
                     /locus_tag="IVW29_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838975.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13634..14152
                     /locus_tag="IVW29_001488"
     CDS             13634..14152
                     /locus_tag="IVW29_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838976.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14133..14525
                     /locus_tag="IVW29_001489"
     CDS             14133..14525
                     /locus_tag="IVW29_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7838977.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14601..15536
                     /locus_tag="IVW29_001490"
     CDS             14601..15536
                     /locus_tag="IVW29_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7838978.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15669..16448
                     /locus_tag="IVW29_001491"
     CDS             15669..16448
                     /locus_tag="IVW29_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7838979.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16441..16758
                     /locus_tag="IVW29_001492"
     CDS             16441..16758
                     /locus_tag="IVW29_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7838980.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17002..18612
                     /locus_tag="IVW29_001493"
     CDS             17002..18612
                     /locus_tag="IVW29_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7838981.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18769..19617
                     /locus_tag="IVW29_001494"
     CDS             18769..19617
                     /locus_tag="IVW29_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7838982.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6350 a         3513 c         3956 g         5829 t
ORIGIN      
        1 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
       61 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
      121 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
      181 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
      241 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
      301 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
      361 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
      421 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
      481 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
      541 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
      601 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
      661 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
      721 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
      781 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
      841 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
      901 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
      961 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     1021 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     1081 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     1141 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     1201 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     1261 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     1321 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     1381 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     1441 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     1501 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     1561 tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     1621 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     1681 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     1741 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     1801 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     1861 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     1921 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     1981 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     2041 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     2101 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     2161 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     2221 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     2281 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     2341 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     2401 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     2461 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     2521 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     2581 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     2641 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     2701 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     2761 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     2821 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     2881 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     2941 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     3001 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     3061 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     3121 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     3181 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     3241 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     3301 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     3361 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     3421 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     3481 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     3541 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     3601 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     3661 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     3721 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     3781 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     3841 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     3901 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     3961 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     4021 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     4081 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     4141 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     4201 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt
     4261 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     4321 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt
     4381 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     4441 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     4501 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     4561 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     4621 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     4681 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     4741 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa
     4801 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     4861 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     4921 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     4981 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     5041 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     5101 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     5161 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     5221 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     5281 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     5341 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     5401 ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     5461 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     5521 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     5581 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     5641 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     5701 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     5761 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
     5821 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     5881 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     5941 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     6001 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     6061 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     6121 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     6181 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     6241 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     6301 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     6361 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     6421 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     6481 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     6541 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     6601 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     6661 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     6721 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     6781 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc
     6841 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     6901 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     6961 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     7021 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     7081 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     7141 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     7201 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     7261 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     7321 tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc
     7381 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     7441 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     7501 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     7561 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     7621 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     7681 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     7741 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     7801 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     7861 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
     7921 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
     7981 aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca
     8041 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
     8101 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
     8161 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
     8221 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
     8281 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
     8341 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
     8401 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
     8461 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
     8521 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
     8581 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
     8641 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
     8701 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
     8761 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
     8821 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
     8881 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
     8941 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
     9001 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
     9061 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
     9121 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
     9181 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
     9241 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
     9301 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
     9361 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
     9421 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
     9481 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
     9541 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
     9601 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
     9661 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
     9721 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
     9781 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
     9841 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
     9901 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
     9961 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    10021 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    10081 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    10141 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    10201 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    10261 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    10321 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    10381 cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    10441 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    10501 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    10561 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    10621 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    10681 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    10741 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    10801 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa
    10861 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    10921 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    10981 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    11041 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    11101 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    11161 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    11221 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    11281 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    11341 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    11401 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    11461 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    11521 aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa
    11581 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    11641 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    11701 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    11761 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    11821 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    11881 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    11941 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    12001 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    12061 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    12121 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    12181 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    12241 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    12301 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    12361 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    12421 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    12481 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    12541 agaggaaaca aaaatacatg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    12601 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    12661 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    12721 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    12781 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    12841 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    12901 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    12961 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    13021 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    13081 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    13141 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    13201 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    13261 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    13321 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    13381 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    13441 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    13501 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    13561 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    13621 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    13681 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    13741 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    13801 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    13861 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    13921 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    13981 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    14041 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    14101 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    14161 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    14221 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    14281 gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt
    14341 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    14401 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    14461 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    14521 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    14581 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    14641 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    14701 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    14761 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    14821 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    14881 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    14941 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    15001 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    15061 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    15121 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    15181 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    15241 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    15301 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    15361 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    15421 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    15481 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    15541 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    15601 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    15661 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    15721 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    15781 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    15841 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    15901 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    15961 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    16021 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    16081 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    16141 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    16201 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    16261 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    16321 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    16381 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    16441 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    16501 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    16561 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    16621 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    16681 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    16741 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    16801 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    16861 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    16921 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    16981 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    17041 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    17101 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    17161 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    17221 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    17281 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    17341 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    17401 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    17461 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    17521 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    17581 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    17641 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    17701 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    17761 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    17821 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    17881 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    17941 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    18001 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    18061 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    18121 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    18181 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    18241 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    18301 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    18361 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    18421 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    18481 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    18541 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    18601 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    18661 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    18721 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    18781 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    18841 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    18901 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    18961 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    19021 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    19081 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    19141 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    19201 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    19261 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    19321 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    19381 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    19441 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    19501 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    19561 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    19621 cttttattca aaaaacatac caaaaata
//
LOCUS       DACCVU010000048        19001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCVU010000048 DACCVU010000000
VERSION     DACCVU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.048"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..561)
                     /locus_tag="IVW29_001495"
     CDS             complement(<1..561)
                     /locus_tag="IVW29_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7838983.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="IVW29_001496"
     CDS             complement(748..1398)
                     /locus_tag="IVW29_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7838984.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IVW29_001497"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IVW29_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7838985.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="IVW29_001498"
     CDS             complement(2910..3908)
                     /locus_tag="IVW29_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7838986.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IVW29_001499"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IVW29_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7838987.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="IVW29_001500"
     CDS             complement(4924..5655)
                     /locus_tag="IVW29_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7838988.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="IVW29_001501"
     CDS             complement(5659..6924)
                     /locus_tag="IVW29_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7838989.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IVW29_001502"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IVW29_001502"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7838990.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IVW29_001503"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IVW29_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7838991.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="IVW29_001504"
     CDS             12266..13768
                     /locus_tag="IVW29_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7838992.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="IVW29_001505"
     CDS             13770..14507
                     /locus_tag="IVW29_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7838993.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            14513..14671
                     /locus_tag="IVW29_001506"
                     /pseudo
     CDS             14513..14671
                     /locus_tag="IVW29_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(14678..15871)
                     /locus_tag="IVW29_001507"
     CDS             complement(14678..15871)
                     /locus_tag="IVW29_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7838994.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15950..16156)
                     /locus_tag="IVW29_001508"
     CDS             complement(15950..16156)
                     /locus_tag="IVW29_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7838995.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16149..17291)
                     /locus_tag="IVW29_001509"
     CDS             complement(16149..17291)
                     /locus_tag="IVW29_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7838996.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17803..18438
                     /locus_tag="IVW29_001510"
     CDS             17803..18438
                     /locus_tag="IVW29_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7838997.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18592..18669
                     /locus_tag="IVW29_001511"
                     /pseudo
     CDS             18592..18669
                     /locus_tag="IVW29_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18914..>19001
                     /locus_tag="IVW29_001512"
     CDS             18914..>19001
                     /locus_tag="IVW29_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7838998.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYH"
BASE COUNT         5501 a         3991 c         3319 g         6190 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattcctc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaaaat aaattatata
    14521 tgtctttttg actatctttt tatatcttct taaatgtccg caaagcctta ttttataggc
    14581 tctacgggca ttttgctttt gtggtaaacc tcacatatct aggtctatct tcttatattt
    14641 tcactatcaa gcgtggttaa aatcgtggta aatacttcta tgcaattaga cgctgaacct
    14701 ctgattttgc ggtatctatg gacgcatgag cataccagtt cattgtgata ctgatatttg
    14761 aatgccccat gatatactgt aaatcttttg ggttcatgtt cttgcttgcc agtcttgtgc
    14821 agaatgtatg gcgtagcgta tgcggtgtga tatgtggcaa tggattgtcc ttgtggtgct
    14881 tgttgtattt ctttaccata cggataaata aggcgttata atcaatcgca actttgggct
    14941 tgcccttatg attgacaaat aggaagttgc cccgtccgtc tatcacaaat ggttctgcct
    15001 ttggacgttt tttcataacc tgttgaaatg cctgtatcgt ttctttgctt aatggtactt
    15061 gccttattcc gctttttgtt ttaggcgttt caatataata gccctgttcc ttgctcttta
    15121 gtaactggtg gtcgataatc acaacttcat tcttgaaatc aatgtcggct actgtcagtc
    15181 cgcacagttc cgaaatacga agtcctgtct ttaacagtat cagcacatca tcataatgct
    15241 tgtgatacac attgtccgtc ttgatgaatg agagtaaggc ttgttcctgt tcctctgtca
    15301 atgcgacttt ctttttggta tcattttcta ggacttcact caacttgaaa tcaaagggat
    15361 ttttcctcac ataatcatct tgaatggcta tgtagaatga cgcttttaac gagcgtttat
    15421 ggttgttaat ggtattgtag gaaaagcctt tgtctttcat gcgtaacgcc cattccttag
    15481 cgtcagaggg tttgattgta tcaatgctcc tagcacctaa cttgtcctct ttcaataacc
    15541 gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac atttgccctc tgtgcgttct
    15601 gtttggcata aagttggcaa agcgtcattt tcttgccagt gctgtcgata ccgtcctcaa
    15661 tatcccgtct tatctgctgt tccagttctc gaagtgaggg tttttcccgt tttccctttg
    15721 gtgtcgggtc tgtgggtgtc aatctccaag catacacata ttttgtgttt ccaaatgcgt
    15781 ctacatattt atataagtat tttctgtctg ttcgttggct ctctccagta tgcaggatac
    15841 gacctttgtt atcccgtctt ttttctttca tggtgtctgc tcctttcctt gttggaaaga
    15901 gccttgatat gacttgtgtt catcataaca catacaaggc tcatttgcat tagatagcgt
    15961 ccaatttatc aatgacctgt tcaaactgtc ggcgtttaat ctgtatgcgg ttgccattca
    16021 taatgagcca gccagcgtcc ttgttttcct ctgccaatcg tcttaacttg ttttctccga
    16081 tacggaaata ctttgacgct tcttcaatgg taagggtgta tttttcccat acgggaatat
    16141 cctgtgcatt actcataaaa caccccctca ctttcgatta cgtctgctat gtccgtacac
    16201 atctgttcca caagcatttc ctgcttttca gagaggtggg tacattcatc aatcattttc
    16261 atgtagtttg agcctgtcag cctgtcgatt tcctctatca tcacgattga cggcatgacc
    16321 tgtctttcca gccattgcag agtgcgttcc atattgacag gtacggggtt catttcaaaa
    16381 ggtaaactgt catgggaaat gaatatctcc cacagcggat agtcgggaaa ttgcacaaaa
    16441 tcagtaataa ggtaaaacac cagcccatga gggttatatg tcaataacaa ttcctcaact
    16501 gcctgtactg cttttgtacc acgcagacga atttcaaaac gattgataat atccgtatgt
    16561 ttctttttgg ttgcctgttc cttttgtttt tcatacaggc agaagtattt tgtgcttgcc
    16621 tttgaaccga tataaagggt gcttgctaaa ttacggtttt ttccgcttag ttttccaccc
    16681 tgcacatttt cataattttt acagcgacaa tccgcaccgc catttctgta tttttcagac
    16741 aaaacgggaa tatccaacag accacacata tcattgattg ccaagtcaaa acgtcggata
    16801 acaccgtcac agtcaagaca gcggtttaaa aatgaatacc aatctctgtt ctgtgcctgc
    16861 aagacatatt ccatgttgcg tgagcctgcc ccttttaatt ccaagaatac gcccatgtgt
    16921 tcgtcatcag aagccatgac cttgatttct ccgtaggcgt aatgctcttt atagccataa
    16981 tacccatagt cataatggat aaaataatct gctttcatgc caagcacctt tcggataatt
    17041 tccaatgcgt cagtcgttgg ataacggaca gacagataat caacgagcag ataaaaaggc
    17101 ttatcgcaga tataataatt cagacaacgc aggataatat tttgtgtttc cttgctggcg
    17161 ttggcttttc cgttctcaaa gcggttgata ctatatcttg atacatgggt aagacttgca
    17221 agttctcctt gtgaaagacc gcttgcaaga cgtttgttct tcatttcaag tgcaaattct
    17281 aaattgttca ttgtattcct ccaatcccac aatagccttg aaatacctgt aaacctctat
    17341 tcaaaaagtg tgatatgcac tatcttctta aaacttctca ttactactca caactttggg
    17401 attgtatggt ttttgacttg tcttttgtga aatagtgccc ccctgttaga taacgggggc
    17461 acagacaagg ctcgcaggct cgccagccga caaagcattt cttccccttg acacaaggtg
    17521 tcatggcggt aagcctgaga ggcttccgcc accgtgtcag aaatactttg tcagcaagtt
    17581 ctgttttgcc ttttctaaca agttccattt caaccgatat tcatatctac ggatttaatc
    17641 agataggtat ttctttttga atgtttttat atttcattct attgtgctta ttcgattaca
    17701 agaacatagt aattcttata tgcttatttg tcaatatctt tttttgcctt tacaaatcat
    17761 attaagcatg atagacttat aatagcgaag atataacgag atatggaggg ttatgcaatg
    17821 aaaataagcc atttaggaaa taatatacag accataagaa aatttagggg aatgaaacaa
    17881 caggaacttg cggacaaaat cggtatcaat atgcagagcc tttccaagat tgaaagaggg
    17941 ttgaactatc ctgcctatga aacgctggaa aagataatgg aagtgctgga cgttacgccc
    18001 aatgaattgt tgtcgggaga atggaagtat gtcaatcaat ctgaaaagga agtctgtcag
    18061 tttttaagag ccgaagaacg tctaaatgca gaactgaaac atggacacta tgataacttc
    18121 tttgacagcg aggaagaatg gctggaatat gagttagaaa agttacggga atatattaca
    18181 gactatatca acgggaaaag cattgaagcg tccgaccttt acccaatcaa agaatttatc
    18241 cagcatttga agtttcaaaa attattagac cgctatgatg atttatacag tatggatatg
    18301 tttggggaaa gcatagaggg acataagtat aagacacctt atcaagtcgt gaaaatgata
    18361 aacccgaact caaaagaaga tatggaactg ttacgagaag tattaaggaa taacaatttt
    18421 gatgatgagg acgagtaact tttttcctct tgcgagaaga tacagcaaac aaaaagcccg
    18481 atcaagagtg caaagcacca ccaatggtgg caaagctctt gacagggctt tttcttttct
    18541 gttgtgggca atcaagagga agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag
    18601 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
    18661 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
    18721 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
    18781 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
    18841 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
    18901 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
    18961 ggttatgcat aaaaatccca gtgataaaag tatttatcac t
//
LOCUS       DACCVU010000049        18938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCVU010000049 DACCVU010000000
VERSION     DACCVU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.049"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..435
                     /locus_tag="IVW29_001513"
     CDS             <1..435
                     /locus_tag="IVW29_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7838999.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IVW29_001514"
     tRNA            complement(759..842)
                     /locus_tag="IVW29_001514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IVW29_001515"
     CDS             1370..1870
                     /locus_tag="IVW29_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839000.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IVW29_001516"
     CDS             complement(2060..2404)
                     /locus_tag="IVW29_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7839001.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IVW29_001517"
     CDS             complement(2558..3478)
                     /locus_tag="IVW29_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7839002.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IVW29_001518"
     CDS             3665..4156
                     /locus_tag="IVW29_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7839003.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IVW29_001519"
     CDS             complement(4180..6555)
                     /locus_tag="IVW29_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7839004.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IVW29_001520"
     CDS             6881..7507
                     /locus_tag="IVW29_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7839005.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IVW29_001521"
     CDS             7530..8234
                     /locus_tag="IVW29_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7839006.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IVW29_001522"
     CDS             8426..9259
                     /locus_tag="IVW29_001522"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7839007.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="IVW29_001523"
     CDS             9456..11384
                     /locus_tag="IVW29_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7839008.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="IVW29_001524"
     CDS             11522..11977
                     /locus_tag="IVW29_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7839009.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="IVW29_001525"
     CDS             12447..12716
                     /locus_tag="IVW29_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839010.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="IVW29_001526"
     CDS             13116..13370
                     /locus_tag="IVW29_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7839011.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IVW29_001527"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IVW29_001527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7839012.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="IVW29_001528"
     CDS             complement(14543..16816)
                     /locus_tag="IVW29_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7839013.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="IVW29_001529"
     CDS             complement(16809..17348)
                     /locus_tag="IVW29_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7839014.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="IVW29_001530"
     CDS             complement(17484..17702)
                     /locus_tag="IVW29_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7839015.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="IVW29_001531"
     CDS             complement(17699..17944)
                     /locus_tag="IVW29_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7839016.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="IVW29_001532"
     CDS             complement(18060..18788)
                     /locus_tag="IVW29_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7839017.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5815 a         3666 c         3329 g         6128 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaaca aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gatttttgac gaaaaagagg
    18901 ggttgtgaca gaactcgtgt cacaacccct ctttaatt
//
LOCUS       DACCVU010000050        18019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCVU010000050 DACCVU010000000
VERSION     DACCVU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.050"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(24..722)
                     /locus_tag="IVW29_001533"
     CDS             complement(24..722)
                     /locus_tag="IVW29_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7839018.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IVW29_001534"
     CDS             complement(715..2067)
                     /locus_tag="IVW29_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7839019.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IVW29_001535"
     CDS             complement(2290..3690)
                     /locus_tag="IVW29_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7839020.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IVW29_001536"
     CDS             3851..4588
                     /locus_tag="IVW29_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7839021.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IVW29_001537"
     CDS             4713..5192
                     /locus_tag="IVW29_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7839022.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IVW29_001538"
     CDS             5189..5956
                     /locus_tag="IVW29_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839023.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IVW29_001539"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IVW29_001539"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7839024.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IVW29_001540"
     CDS             complement(6859..7461)
                     /locus_tag="IVW29_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7839025.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IVW29_001541"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IVW29_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IVW29_001542"
     CDS             8142..8726
                     /locus_tag="IVW29_001542"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7839026.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IVW29_001543"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IVW29_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7839027.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IVW29_001544"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IVW29_001544"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7839028.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IVW29_001545"
     CDS             10627..10806
                     /locus_tag="IVW29_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839029.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IVW29_001546"
     CDS             10828..11853
                     /locus_tag="IVW29_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7839030.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IVW29_001547"
     CDS             12028..13272
                     /locus_tag="IVW29_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7839031.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IVW29_001548"
     CDS             13425..14372
                     /locus_tag="IVW29_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7839032.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IVW29_001549"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IVW29_001549"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7839033.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IVW29_001550"
     CDS             complement(15274..15516)
                     /locus_tag="IVW29_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839034.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IVW29_001551"
     tRNA            15637..15709
                     /locus_tag="IVW29_001551"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IVW29_001552"
     CDS             complement(15828..16466)
                     /locus_tag="IVW29_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7839035.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IVW29_001553"
     CDS             complement(16589..17221)
                     /locus_tag="IVW29_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7839036.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IVW29_001554"
     CDS             17572..17871
                     /locus_tag="IVW29_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7839037.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6171 a         3155 c         3557 g         5136 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaaca
//
LOCUS       DACCVU010000051        17908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCVU010000051 DACCVU010000000
VERSION     DACCVU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.051"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..480)
                     /locus_tag="IVW29_001555"
     CDS             complement(<1..480)
                     /locus_tag="IVW29_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7839038.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     "
     gene            585..1136
                     /locus_tag="IVW29_001556"
     CDS             585..1136
                     /locus_tag="IVW29_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7839039.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1289..1762)
                     /locus_tag="IVW29_001557"
     CDS             complement(1289..1762)
                     /locus_tag="IVW29_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7839040.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1900..3372
                     /locus_tag="IVW29_001558"
     CDS             1900..3372
                     /locus_tag="IVW29_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7839041.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3369..4079
                     /locus_tag="IVW29_001559"
     CDS             3369..4079
                     /locus_tag="IVW29_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7839042.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4431..4715
                     /locus_tag="IVW29_001560"
     CDS             4431..4715
                     /locus_tag="IVW29_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7839043.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4996..5415)
                     /locus_tag="IVW29_001561"
     CDS             complement(4996..5415)
                     /locus_tag="IVW29_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7839044.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5417..6001)
                     /locus_tag="IVW29_001562"
     CDS             complement(5417..6001)
                     /locus_tag="IVW29_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7839045.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6048..7523)
                     /locus_tag="IVW29_001563"
     CDS             complement(6048..7523)
                     /locus_tag="IVW29_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7839046.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7704..8015
                     /locus_tag="IVW29_001564"
     CDS             7704..8015
                     /locus_tag="IVW29_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839047.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8130..10466)
                     /locus_tag="IVW29_001565"
     CDS             complement(8130..10466)
                     /locus_tag="IVW29_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7839048.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10608..11276
                     /locus_tag="IVW29_001566"
     CDS             10608..11276
                     /locus_tag="IVW29_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839049.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11279..12322
                     /locus_tag="IVW29_001567"
     CDS             11279..12322
                     /locus_tag="IVW29_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7839050.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(12559..13440)
                     /locus_tag="IVW29_001568"
     CDS             complement(12559..13440)
                     /locus_tag="IVW29_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7839051.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            13599..14327
                     /locus_tag="IVW29_001569"
     CDS             13599..14327
                     /locus_tag="IVW29_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7839052.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            14483..14800
                     /locus_tag="IVW29_001570"
     CDS             14483..14800
                     /locus_tag="IVW29_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7839053.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            14793..15401
                     /locus_tag="IVW29_001571"
     CDS             14793..15401
                     /locus_tag="IVW29_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7839054.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15417..16247
                     /locus_tag="IVW29_001572"
     CDS             15417..16247
                     /locus_tag="IVW29_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7839055.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            16385..16873
                     /locus_tag="IVW29_001573"
     CDS             16385..16873
                     /locus_tag="IVW29_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7839056.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            16924..17796
                     /locus_tag="IVW29_001574"
     CDS             16924..17796
                     /locus_tag="IVW29_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7839057.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
BASE COUNT         5556 a         3133 c         3641 g         5578 t
ORIGIN      
        1 atctggcatt ccatcgtatg gttcttcgct aataacatct tcattgtcta tacctgcatc
       61 acccatataa gtgatcgtag caaattcagg aacaagtaat tttggataag tttcatattt
      121 ttcacgagct gcttccattt ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg
      181 atgttcttca acccatttag ggaaaaagat cattccgtga agtttctttt catttggcat
      241 aaagtccaat gccacgtctg tccctgttgg ctctgtccaa gtgattttga atacgccgtc
      301 cgtcaattta acgatatcag cttcttggtc ttttacccaa cggcctttga ccattccgcc
      361 atggatacgg taatcaacag tatggtcatt tttggcatac cattcatatt cccaaccatt
      421 gtcatacgta tagataaagt gagttccaat aaaatcttcc aattctttaa attctttcat
      481 gtttaagcat ctccttataa attaaaacat gtaataaact acatgttttc gattactcat
      541 ttatgataga atggatttaa agatttgtca aggagggaaa actgatggca agaaaaaata
      601 cacttcgata tattttgtta gggttattaa gtaagaagaa gatgacagga tatgaattga
      661 atcaatcgtt caaaaatgaa ataggagaat tttggcaagc aaaacatagt cagatttatc
      721 ctgaacttgc aaaaatggaa gagcaaggaa tcattcagca tcaagtagag atcacaggtg
      781 aaaaattgga aaagaaagtt tatgaattaa cagaagaagg gaaggaacaa ttggcagagt
      841 ggattcatac acccaccaat gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt
      901 atttcgtcaa agacgtgaat gatccggcat tagtagaaat tattcagcag cagcgtgacc
      961 ttcatgaaga aaaacgactt catctattag agcgaaaaaa aacgatcttt cctactgaaa
     1021 aagaaaaaca tgaaaattac ggacactacc tgattttagc gcatgcgatc aatcgggaaa
     1081 cagaatatgc aaattggcta acagatgtat tagaagaaag taaccgaaaa aaataaaaaa
     1141 atatccgaac agtgatggag aattcctttc tatatgaaag aaacgcagat caccgttcgg
     1201 atattcttat gttcaaacag gccttttttc aagtatttat gtagcatcta cttattaaaa
     1261 agcttggtac ttaggagatt agtggcgttt agagttcttt taacaactgt tcgtagtcag
     1321 ctttttcatc gacaggaagg agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga
     1381 tgtacatctt agcattctct tctttgaagt taccgatttg taaaatcgta tagcgttcgt
     1441 cgatcaattc gatatctttg attgcttttg aaaaaacttt acggacatgg tgcattttct
     1501 tttggcggat ttctgccttt tctgcagctt ttgacaaagt aaatccgtca tctaaatatt
     1561 ttttgatcag ttctactttt atgactgtag gtaaacgata ttttctagga caattcgcat
     1621 cagcttcgat cgaacgaatg aatccctttt gttcccaata ccttaattga cgtggagaca
     1681 cacctacgat atcacttaat tcactgatgc caacaaccaa ttgatcattt tccaacaaac
     1741 gtttaaggga agccccaacc atttttgtca ctccttttgt cctcttgctt cccttaatta
     1801 tatccgtatt aacttatgtg tcaagtaggt tgacaaatag cgaatttagg actaaagtat
     1861 tctagtagtt tcagaaaaaa ttcagaaaga aggagaaata tgagtaacaa ccaacccgta
     1921 gatatttacg gaaaaccata taatcggaca cttttagttg ttgtcttatt aattggtaca
     1981 ttttgtaccg ttttgaatca gaccttattg acgacagctt ttcctacatt gatgaaagct
     2041 ttcgatattt cagcgtctga tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg
     2101 atcatgatcc caataacagc atggctgatc aataaattca gttcaagaaa tttatatctt
     2161 tctgccatga cgatcttttt gatcggaacg attacttgct ttacagcacc taattttagt
     2221 acattactaa tcggtcgttt gatccaagct tgcggtgtgg gagtatcgat gccattattg
     2281 caaaatatca tgctatctat tttcccgcca gaaaaaagag gatcagcaat ggggatgtca
     2341 gggatcgtta ttggacttgc tcctgcttta ggccctacat tatctggtta tatcattgat
     2401 aactaccatt ggcgtgactt gtttggtatg gtactgccaa tcgttgttct tgtcttggtt
     2461 ttagcattct tcctaatgaa aagtgttatt ccattgtcaa atcctagcat tgatatttta
     2521 tccgctattt tatcaacgat tggattcggt agtttacttt acggattttc tagtgtagga
     2581 gatgacggct ggggaagctt gaaagtaatt ggtttcttag ttgttggtgt gattttcatc
     2641 gccttgttct catggcgtca attacatttg gaacatccat tcttggaact tcgtgtattc
     2701 aaatcatcta cattcacgat tgcagctgtt ttagctggtg taacaaatat ggcgatggta
     2761 ggggcagaaa tggtcgtacc attgtatatt caaaatattc gtggagaatc agcattccat
     2821 tctggcttga tgttgttgcc gggtgcattg atcatggggg ctatgatgcc tgtgacgggt
     2881 gcgattttcg ataaatacgg cgcgaaacgt ttagccatca cagggatgtt cattttgaca
     2941 gcggcaactg caccatttgc tttcttgaca aaaacgactc cagttttata tatcgtgatc
     3001 ctttatgcga tccgtatgtt tgggatctcc atggtcatga tgccggtaac gacttctggt
     3061 atgaatgcat tgccaaataa cttgatcagt catgggacag cggtaaataa tacattccgt
     3121 caaatcgcaa gctcgattgg tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa
     3181 gcaaatatcc caagcaaatc tgtattggat aatacgccat tagcttataa agatcaggca
     3241 atcaatgcca cactgtcagg ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt
     3301 cttggcttta ttattgcctt tttattgaag aaaaaagaac ctgttctaat gaaagcgggg
     3361 gataatgcat gattattgga gttttgattg tcagcttgct tgcttttgct ttaacgaacg
     3421 tgttcgctaa aaaaacatgg caaacctttt tatctttgat tttcggacta atcttcgtcg
     3481 cttcactaag tttgatcgtc gctaatttgt ccaatcactt tggaatggaa aaagtaacgg
     3541 aaacaaaaac agaaaaaatt gtttccagtg ctgattccca aggagcagat atgcttttgt
     3601 ataaagcttt agggaatgga aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag
     3661 aaaaaccaaa agcaactggt acggataacg aaacaaataa agtagaaaag acagacggcg
     3721 atgctgaaaa agtgacgaaa actacttatt gggaatacaa aaacgacatg tataaattct
     3781 ggttcaatat tgctgataac aatcacgaat atgacagccg agtgaataca ttcaaaatcc
     3841 cagagacatg ggtagaatta agtacggacc aagcagccaa gttagctgaa ttagtgaaga
     3901 aacagcaaag tacgatggaa agtgaagcaa aagcttatgt tcaagacggt atggtaaaag
     3961 caatgactga aaatccaaaa atgagcaaag cagagcaaga acaacgtacg aaagccttag
     4021 ctgctgaatt ccaacaacaa gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat
     4081 attttgaaca ccatgtagga ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa
     4141 tcagataaaa atagcactcc atatttttca tctgattggg cttagtgccg tagatgcaag
     4201 cttttgaaca ccgtttaagg aagaggttgt gagcctgccg tttaactctg agtcacaagg
     4261 agcaatttca aaaaacagct tctcatgttt gttgactatt gtgacttgtg accgaggagt
     4321 tggcaggaga acaccgttta ttcgaaaaaa ggaactgcga caagactttt gtcacagttc
     4381 cttctttttt ttgctatgct atactagatg taaaaaatag gagaaataaa atgagggaaa
     4441 tttattcttt tgaatcaaaa atctatgctt ccgaagtcgg caagggcggg gcatatatta
     4501 tttttccgta tgatataaga aaagaattcg gtgctggaag agttaaagtc caagcaactt
     4561 ttgacaatgt gccgtatgaa ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt
     4621 cagtctgcta catccttgga ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg
     4681 gtgatattgt agcagttaca gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc
     4741 acaacctctt gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     4801 aaaaatgtgg aaacccatgt ttacaaaatt tttcttacta ctcgaaacga agcggcttta
     4861 tcacaacctc ttgataaacg gcgttcaatc agctgtccct cggtattagc tcaaaaacgg
     4921 caaaatatga gaagctattt ttccattttt ttctctcata ctcaggtcat aacgctgatt
     4981 tcacaacctc ttctttcata atggtttttc accatcttta taataaactg gttctttgat
     5041 atggaaatct attttttcca accaatctaa aatcttgtaa cctaactctt ctgacatcgg
     5101 aaaagaggcg ttgatttgtg cattggagat atggtcaaca tggataccgg aagtgatcgt
     5161 acaacttccc ggaacggcag ttttgatgat cacagctatt ttttcggcta agacatcgtc
     5221 tttatggaac cgaccatcgt ggctagggaa acgtacagtt tgcatctctg tatcacgaga
     5281 aaatgtagtg actgttccga tatgtggtgt atctccgcct gttaaagtga tcaataagtc
     5341 tttaccgatt actttaacag cagcttggat cgtgaaacct agtttggtta cttcaactat
     5401 cgtttctttt tccatattaa tcaccctgcc aacgttttga atgctcattt acaatttcaa
     5461 attgatccaa aagtttcatt gtatggtgat taacaagatc ttggatcgtt tgaggctgat
     5521 tataaaaagc aggaattggt ggtatgattt gcacacctag tcgtgctaat ttagtcaagt
     5581 tttccaagtg aatggcattt aatggcgttt cacgaggaac aatgactaac tttcgttgct
     5641 ccttcattgt tacatcagca gcacgtgcaa tcaggttgtc gcctaaacca acagagatac
     5701 ctgcgactgt tttcatactt gccggtacga tgatcattcc atcggtcaaa aatgatccgc
     5761 tggcgacagc agcacctaaa tctttgtttg aataatggta atcaaacagg ctttcgaatt
     5821 cagttagtga taagtcggat tccagcttta agttctgttt tgcccatgga ctgatgattc
     5881 cgtgtgtttc tatcatcgga tattctttta gtttcttcac taggttgatg gcataaatgg
     5941 tgcctgaagc gccacttaca ccaatcacga tttttttctt ccgttgtgcc aattgttcca
     6001 ttttccgcac tcctttttat gatgtcaaaa cagttgcaga tcattcatta tttcaaatat
     6061 ttttcccaat cttctacttc ttggaatttt gcacgtacaa aatgttcttt catatcgaaa
     6121 ggaactgtac cgtcaaagat agtttttgca cttatcccac ggaaacgaat agagccagga
     6181 ttatattctg gtaattctga tgggtccaat ggatggttcc tcattcctgg gatcacgatg
     6241 atatctttat ctccttggaa acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa
     6301 atatcaacat cttcatcgac taaaatgact gtcttcaatt ctttgaaggc tgataaagca
     6361 agaatagctg cttgacgctg aataccttca tccgcttcgt tttctttacg gatttgcatg
     6421 atggtcatca atttaccacc acctgcagga ggattgtaga catttactac ttttcctgga
     6481 atagctttat cgaccaaggc aagaatactt gcttctgtag gaataccggc catagatacg
     6541 tgttcttcac tgggaccaat cgttgtttgc atgattggat tgtcttttcg gtgagtaact
     6601 gctttgactt tcactacttg tacagctgga ttagcatccc cagtatagcc aggaaattct
     6661 ggcattgctt ttcctgagtt cgtgttgata tcttcttgca ttgtttcatt tggcatgatt
     6721 tctgcttcaa tgatgaattc agcacgagca atacccactt catcgactgc tataccatca
     6781 acgatttgaa taggttcgtt acgcaaagct cctgcacacc atagttcatt gtagccaagt
     6841 ggagtggtag gtggttcgaa agtcgtgcca atggcaattg ctggatctaa accaatattg
     6901 atagtgatag gcattggttt attcaaggct tcgaattgtt tttggaagtg accaatgtgg
     6961 cgtccgccag gcatgatgta cataccgatc gtatctttat cttctagaac catacgatgg
     7021 atcgttacat cgctcattgt tttttctgga tctgatccta agactacacc cattgtgatg
     7081 tatggaccag catcttcagg agtatttgta ggtgcaggca aaatgttgcg aatatcgaag
     7141 tctgcatcgg tagctagatg aacgacttct tgtgctggag cgtctgcttt gtctactttc
     7201 acaggtttga ttggattttc tacagcgtct ttcaagaaac gaccaagtgt tcgataatca
     7261 tggtgcaaaa tttcacctac acgtttacga ctagccattg ttccgatgtt cacacgtgtg
     7321 ccggggaaac ctttgatatt gttgaaagtc atagctggtc cttcttgagt cggacgtttt
     7381 actgtaccgc cagcaccgat ataacggtaa acaccagata attctgcttc aggatcgatt
     7441 tcaatatctg tttcgtgata ttgacctgga agtgttttta gttcttcgat gacttttctt
     7501 aaatcatacg gttgatctgt catgagagat tcctccttag ttgtctttac ctatctatag
     7561 taaaaggtga aaaagaggaa aacaattcaa cttgttcggt aaagtattct acaatggaat
     7621 ggatgcgttt ttttgataat agaataaact atgatatact tatcactaaa agaaagcgca
     7681 tacttagtgt tggaggaagg aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa
     7741 aagtggaaaa tatagcgaat attttgaaag ttacatttga aaacggagaa gtaaaatacg
     7801 tgaaatctca ttggacagaa gagataaccg atgccttgca atttggaaaa aaaggaagag
     7861 gaaaaagaaa aaatcttctc gcactttctc gtaatatgtg gattgggact gaagtaacga
     7921 ttgaagcaga tggaacagta ttcataaatg gaaaagacag gtatacgcca gaagagttgt
     7981 ggtacaaagg aaagaaaagt ataccagaat tataaaaaat aattaaaaaa aacagcgcga
     8041 aacaaggaac atattacttc ctcgtttctt gctgttttct actatttcag cgctcttatg
     8101 actcaaatct aaatgtggtt gtcacaacct tattctgcac gcaaagcgat agcggcgtct
     8161 tttttcgctg ccattcgtgc aggaagatga ccgccaagca tcgtcaatat agtagagacg
     8221 atcactagaa taatcgcatg gacaggattt agttgagcaa cattttttag atccgtcatg
     8281 ttgtataaga tgctattgat agggaacgta gctagccatg caataaacac acctaatatc
     8341 ccagaagaaa tacctaaaat acacgtttct gcgtcaaata cgcgagtgat gtctttttta
     8401 cgtgcaccta aagcttttag tacaccgatt tcttttgtcc gttcaatcac agatgtataa
     8461 gtaatgatac tgatcatgat catacttgtt actaaagaga tccctgcaaa agcaataagg
     8521 acataagtga tagcatccat caatccgcca gtcagttcag tcatcgttcc agctaaatca
     8581 gtataaatga ttttatcttc atctgatttg cctttattgt aatcatctag ataatccaag
     8641 atcttttctt tgtcttcaaa attgttcgga taaatcatga tacttgaagg caagctgtct
     8701 ccacctaaat aactaagcaa tgtttgtttg gcattttcgt caactttttc agtagttaag
     8761 acatttacac cactgtctct ttgagctttg acgatttctg attctttgtt ttcgttgacg
     8821 atttgagtcg ttaattgatc gctataagcg attcctggag ataagagatt cattgtagaa
     8881 gaagatttca cgcgtaggat ccccgagatc ttcaattcat cagaagcatt ctgatacatc
     8941 gcatcataat ctgtattagg gataaaattc ccagttggaa gttttgtata gtaagtattg
     9001 ttgtttacta atttgaatgt tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct
     9061 ttcacatcaa atccaagatt cttcaatgcg ttgatattag tattattatt gccgtctacg
     9121 atcagcacga catctgttgc tgaggctgga taacttccgg caagtaaaga ataattgtct
     9181 tttaaaaagt tttctttgct agtgtcaagc tgtgtaggga aacttgacac tcccacacct
     9241 gtcattgctg acatcgtact agataaagaa agagattccg tatcaggatt ttgattcgag
     9301 aagctgacag gctgtacttt gccatttaca tcacgtaaaa gattgattcc tgtagtacgg
     9361 gtaaatccga tattattact taagttagga tcgatgtctg ttacgtaatc gacgtatgtt
     9421 tgatcaatct tattcgtgtg ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt
     9481 ttgctgtctg gataatccgc tttgcttgca cccaatccgg catcgtcacg agtctggtta
     9541 gtcgttacct tagaaatagt aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg
     9601 atttgttttt ggaatccaga agaaagggac agaacgatcg cgattccgat gatcccaata
     9661 ctggaagcaa aggaggtcaa gaaagtacgt cctttttttg ttcggatatt gttgaaggaa
     9721 agctttaacg ctgtccaaaa actcatcttt gttctgcgta agttaaattg atcatctttt
     9781 ggtcgttcga tgtgaggatg ggaatctgtt aagatcttac catcagaaaa ttcaatgatt
     9841 cgatctgcgt attgatgggc cagttcagga ttatgtgtga ccatgatcac taatttttct
     9901 ttcgataact cttggatcaa ttccatgatc tggatacttg tttctgtatc taacgctccg
     9961 gttggttcat cgcacaaaag gatatctggg tcattggcta gtgctcgagc gatggccact
    10021 cgctgcattt gtccaccaga taactgattt ggctttttat gcatatgatt tgtcaagccg
    10081 actcggtgca atgcatcttc tgcccgtttt cgtttttcgt ctttagaaac accacttaat
    10141 gtcatcccaa gttcaacgtt ttcaataatg cctagatgac caatcaagtt gtaactttga
    10201 aaaatgaaac cgatagagtt gtttcggtag gcatcccagt cactgtcctt aaagtcttta
    10261 gtagactttc cgttgattac catatcaccg gaatcataat tatctagtcc accaatcaca
    10321 ttcagcatag tcgttttacc agaaccacta ggacctaaaa tcgctacgaa ttctttttgt
    10381 ctgaaagcga cagacacacc atccaatgct ttggtggtgg tttcgccgac tttataatat
    10441 ttttttatat cttttaattg aagcataatc acccttctcc cgtattgaaa tattcggtat
    10501 actaataagt atatcagaac aatgatcgta gtatacgaaa taaatgttaa ctgaagatga
    10561 actacaagaa aaaatctaga aatttttcaa aaaacagagg gagatcaatg aatcgtattt
    10621 tagtagtaga agatgatccg caattacagt tgctttaccg ttctgtgttg gaacgagcgg
    10681 gcttttcagt catcaccgca ttgaatggag aggatgcgct gaaaaaacta gaagcgacac
    10741 gtatagaatt aatcattaca gatatcatga tgccgaaaat ggatggctat gacttgctag
    10801 actcattacg tagcaccaga tttgacacac ctgttctaat gattacggca aaggccgatt
    10861 ttgaggataa gaaacgcggg ttcaaattag gagcagatga ttatatgaca aaacccatcg
    10921 atgtcaatga gatgatccta cgtgtggaag cattgcttcg acgttcaaag atcaattaca
    10981 gcagtagctt gataatcgga caaacaaaat tagagctaga tgcttataca gtgttacaag
    11041 atcagcattc gttgattctg cctcaaaaag aatttctgct tctgtataaa ttactgtctt
    11101 atcccaacaa gatcttcact cgccaacagt tgatggatga tatttgggga ttagatacga
    11161 atacagaaga acgaacgatc gatgtacata tcaaacgttt aaggacccgt tttgaaaaga
    11221 cggaagattt tcaaatcatc acagtacggg gactaggcta taaggcggtt atttaaagat
    11281 gaaaaaaaag ctattttcac aattatggat ctattttgct agtattgtct tcatttcgat
    11341 cttagtgaca ctgctgtgtt ttctcggttt tattttctta ttgtctcgac attctatctc
    11401 gcctgctgaa tcggctggaa aaccaacgtt atacccgtta ttcgtctttg cggggttcag
    11461 tatggtcgtg ggtacaggaa tatcgatttt tgttgggcgg cgtatcctgc atccaatcag
    11521 tgcgctaggg acaaatatga gtctggtagc aacgggagat ttttcgattc gaatggatga
    11581 acagcagaaa gtagcagaag tacagcaact gtataaagat ttcaacgtta tggtccaaga
    11641 actcaatagt atcgagacac tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa
    11701 aacaccacta gcaacaatcc aaggatatgt tcaattgtta caagccccaa atctctctga
    11761 cgaagaacgc caaatatttt tatatcggat tatcgaaagt atcacgcagc tttcacaatt
    11821 aacagagaac atcttaaaat tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa
    11881 agagtatcga ttagatgaac aaatcagaga agtaatcgtt ttttttcagc ctaaatggga
    11941 gaaagaacag ctagaattgg atatcgaact tgctgcagta aattacacag gcaatgaaga
    12001 gtttttgtac caagtgtggc tgaatatcat ggataacgcc ataaagtaca atcaaatcaa
    12061 tggacagatt catatcaaac tatttgagac agccacagag attgtactgg aagtgaccga
    12121 ttcaggtgta ggaatgaatg aagagaccag agatcgaatg tttgaaaaat tttatcaagg
    12181 agatacaagt cgtcaaattt ctgggaatgg gctaggactt tcactggtta aaaaaattct
    12241 agaactgcat gatggacgta ttgattatag cagtatcgaa ggagtaggaa cgacagcaat
    12301 gatacggtta agtaaacaat aggaaaaaga ataaaacccc taaaaattct attgaaggtt
    12361 tttaggggtt tttagtcgtt tttttgtgat tagggaaatg ttcttacggt tcttttggca
    12421 taaaaatcgc tgctttctta ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg
    12481 acatgatgca agaaagagag cgaaagtcat gcgtcgctct ctttttttgg gtggatagat
    12541 ggacagaaaa tgtagggctt agttttcttc ttgatttaaa tactcccaaa atgtatctga
    12601 agccaaagaa tgctcaccga ctttacgagt aaatgcaaga taactctctg ttgttggaga
    12661 aatagggaga atcacagtat tttcttttaa tagattcttg actgtttttt ccataacaat
    12721 agcaatcccc atgtcatttg caatcatatt gatgatcaag tcgattcttg ctcctttata
    12781 agagattttt ggttcaaatc cagcttcgcg acacagatct agtacaggtt gatacaagtt
    12841 cgtagtgggg ccaaggagca aaaagttctc ctttttcagt tcgcttagat ccaatttctt
    12901 tttactagct aaaggatgat ttttaggtag aactgctaca aaatcgtctg tttcagtagg
    12961 caaccactca atcgaacttg gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact
    13021 atgttcattg gcgtcattgt aagttaattc gacactttcc acttctttca actggactgt
    13081 aaattcagga tggcgtttca aaaactcggt gattttggaa aaacctttat aattgatcat
    13141 tgtcggtatc gttaagatat ttaatgaaag attgttgtct ttcgtatagg tttcaagcac
    13201 ttcttgcata gcatggtatt gttctacgac attttttaca tatgggagca ataagcttcc
    13261 agcttcggtc aattcgattt ttctatgggc gcgttcgaat aatttgacgt ctaattctgt
    13321 ttctaacgaa agaatctgtt tagaaatatt tccttgggtg gtgaattgtc gatcagcagt
    13381 tttggtgaaa ttcattgttt cggctaaatc aagaaaaact tcgaatttat ggatatccat
    13441 catattcctc ctacttattc caataaagaa tactttaaca caaaaaatga attggtgtaa
    13501 tcttctctgt ctgttacaat ctttcttgca gcgaaagcaa taattacaga aatttttctg
    13561 atttttttcg cttcgtagaa aaagagagga gttttttgat gccaactgga gttttgatca
    13621 atgtaggttc cgttttgttt ggcggactaa ttggcggatt agtaggaaat aaattaagtg
    13681 aacattttaa agcacagctg acgatggttt ttggtgtgtg ttcgatgggg atgggaatct
    13741 attccattgc gccgatgaaa aatatgccgg ctgttatttt tgctttagtg ataggaacag
    13801 cgattggttt gattgttcat ttaggtaatg ggataaataa gggggctgcg ctgatgcaag
    13861 tacctatttc taaaatcttc cctagtgaaa agcttggtat gacccacgat gaatttatca
    13921 gcacattagt gacggtcatt gttctatttt gtgcaagtgg tacggggatc tatggaagtt
    13981 tagattctgg gatgacagga gacagtacga ttttgatttc taaatctgtc cttgattttt
    14041 tcacagcagc tatttttgcg tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc
    14101 aattcattat tttttatatt ttgttcttat tggcaaaatt tatttatcca ttgactacac
    14161 cagacatgat cttagatttt aaagcttgcg gcggattctt gatggtcgct accggtttta
    14221 gaatgatcaa cgtgaaaatg ttcccagtag ctgacatgat acctgcaatg atcgttatca
    14281 tgccactgag ctggatgtgg acaaattgga ttatgccact gttataataa aaatcaaaga
    14341 gaagccattt tttgctgact aaaaaggatc aaatctttta gtcagttttt ttgtatttga
    14401 caaactgatt ttgaaactat attatatgtt gtataatata gattgaaata aaatattaca
    14461 cttgagaata gaaggtgacg taatgcacat ccagattcca actgttcttc ttgatggtac
    14521 tgtgctagca gcattaagca aggaagatat gtatgggtat gccttaacaa aaatcgtaca
    14581 agaaagtctc tctgtcagtg aatctacact atatccggtt ttgagaagat tgaaaaaaga
    14641 aggatatctt gagacctatg atgaaccttt tgaaggcaga aaccgtcgat attactcgtt
    14701 gacaccaatg ggaagtcgtc atttagaaga aattattgat gaatggattg tttttcgaga
    14761 ttctttagac cggttattgg aggagagcac aaatgaatga atatttgaat gaagttgtgg
    14821 atcatcttca agaaattcct gaagacgatc gttttgattt gatccagtat tatgaggaat
    14881 attttttaga ttctggaaaa acattagatc agatcattga tgaatatggt acaccaaagc
    14941 agtttgcctt aaaattaaaa atcagttact tttcagaaga agaacaagca gaaaacgagt
    15001 tagtgaaaaa tccaaaaaga caaattcgcc tgatttggtt aatcattatc ggtttatttg
    15061 cttctcctgt cctcattcca ctagcactgg ggtttatatt ggttattggg ggactattgg
    15121 ttgctttact agcgctcatt tttagtatct atgttttatg tatcagtatc ttaggtgttg
    15181 gcctattcat gatcatctca ggaatcagtg tgtttggaca atcgattctg agtggattat
    15241 tctttatcgg tgtcggtctg ttcgccacag gaagcgcagt atttatttcg ccactgttgc
    15301 tgaaaggaac aaaagggctt ttttatgtca tgatgaactt tgttaaatgg atgggacgac
    15361 gttttattac aaaaagagat ctgcattcag cgggcgtttg aaggaggaca cataaattga
    15421 gattgaaaca ttttttgatt accggtattg gtctgatgct tactggtggc ctcctagcag
    15481 gtgcaactta tacattaggc gctcaaaaga gtctgacttg ggataatggt cctcggttga
    15541 tcgatatggt tactgagaca aaaaaaatca aggatgcaaa caaaatcgtc atccatgcag
    15601 aaaatcaaac agtttcggtc atacgcggat tggattttga gataaaaacg acttatgata
    15661 agacagaaaa gccagaaata aaggagaaaa atggcgtgtt gactgtgaat gcagtcaaaa
    15721 aatcaacagg tgcaatcatt agtttagaag acacaagtgc acaaatcact attgttgtcc
    15781 ctgcagatca tgaattatct gaattgacaa tcgaagggaa taatagtgca attgatatca
    15841 acgaagtatc aatcaaaaaa gtgaatacta agacacatga tggctgggta tcgttcaatg
    15901 aatttacagg aggaacaata actgcgaaag atgaattagg ctcacttcga ttagatgagg
    15961 ccaatgtgga taacttgatc gtcacaggtg aaaacagcaa cttccatcta gaagacagtt
    16021 tgatcagcaa agaaggaaaa gtcatcctaa acggtggcaa tttatctttg catgagatgg
    16081 aaaacagtgg ctatactatc caaacgtctg gtgaatctta tattgaaaaa gactaccagc
    16141 caataacaaa tactttgtta gaacaaggac agaaaaagct aaagatcact gctaaagaca
    16201 catcgatcaa tattacgacg gatcaagagg acaatgaaac agattgatcc tgtactcaac
    16261 tggatatttt aagaaaaaac agcaaagatc cttccaatct ttgctgtttt tcatcgctta
    16321 acgcacataa ctatagtaaa ataagaaaga aaatatctaa aaagaaaaca gggtgaaaga
    16381 gtatatgaag gtagaagaag gtcaaagaga aaagttaaaa acagaaatgg aaagattaca
    16441 tacttatatc actcaactaa gccaaacctt ctatgatcca gacaaagaaa aagtgatggt
    16501 taattacccg aacaattcag aaggtcgcca attagagcaa gtgtaccatg aagttttcaa
    16561 acatttattg acggtgaaga aagagctgga ttattattct ttaccgatta tcgatacagg
    16621 aatattgaag tatgatggga agaaggaacg atttatcttt aaatctgttc gggaagattt
    16681 gcctctttca gcagggatgg atctagaggt cttggtagaa gactatttta cagaagaaaa
    16741 gcactgggtt cgaacaaaat tggattattt accgcaggca gccggaggga cacaggcaag
    16801 cggttggtac atcactgaag acaaagagtt agaactagaa ggtgtaatgg cgcgtattcg
    16861 aaaaaaaaat taaaaaagta acattagata tttattgaag gataaaggag cgcaggaagt
    16921 agcatgagag aaaaaagagc attttatatc aatggttatg cagggattgt cggattggtc
    16981 gtattagctt tagttggatt gtttttcttc tatttaggta tgtggcaagc gaaagtttgg
    17041 gcactttttt taagtatttt tctttggttg atcacgttat tgttgttaag ttcggcaact
    17101 gtagtcagtc ctaatcaagc aaaagtaatt ttgttttttg gacaatattt aggtacgatt
    17161 cgagaaaatg gctttttcct tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa
    17221 gttcggaatt tcaatagttc tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa
    17281 atctcagcag tagtagtctt taaagtcgtt gatacagcaa aggcattatt cgatgtagcg
    17341 tattatcaag attttgtaga aatccaaagt gagacagcca ttcggcatat cgctagccaa
    17401 tacccgtatg atacattcaa tgagaatgat ttgacgcttc gaggaaacac gacggcagtg
    17461 tctgacgagt tgcaaaaaga gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc
    17521 gaaacacgct tgaaccattt agcctatgca acagaaatcg ctagtgccat gctccaacgt
    17581 caacaagcaa aagccatttt gtctgctcga caaaccattg ttgaaggagc tgtcacaatc
    17641 actcaaatgg ccttagagca aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg
    17701 aaagtccaat tgatcaataa tttacttgtc tcaatcatta cagacaaagg aacgcaacct
    17761 gttatcaata caggcgacat gaaagaccgt aagtgacata ctttttgtaa gataggaaga
    17821 aggagtttag gacggattat tgtcttgaac tcttttttta gttgttttaa agaaaatgac
    17881 aaaattgaaa gaagaatgat agttgatt
//
LOCUS       DACCVU010000052        17470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCVU010000052 DACCVU010000000
VERSION     DACCVU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.052"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            27..545
                     /locus_tag="IVW29_001575"
     CDS             27..545
                     /locus_tag="IVW29_001575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7839058.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            673..1356
                     /locus_tag="IVW29_001576"
     CDS             673..1356
                     /locus_tag="IVW29_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7839059.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1665..1738
                     /locus_tag="IVW29_001577"
     tRNA            1665..1738
                     /locus_tag="IVW29_001577"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Arg,seq:tct)
     gene            1897..2256
                     /locus_tag="IVW29_001578"
     CDS             1897..2256
                     /locus_tag="IVW29_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7839060.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2379..4010
                     /locus_tag="IVW29_001579"
     CDS             2379..4010
                     /locus_tag="IVW29_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7839061.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4072..4713)
                     /locus_tag="IVW29_001580"
     CDS             complement(4072..4713)
                     /locus_tag="IVW29_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7839062.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4995..5303
                     /gene="rplU"
                     /locus_tag="IVW29_001581"
     CDS             4995..5303
                     /gene="rplU"
                     /locus_tag="IVW29_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7839063.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5330..5674
                     /locus_tag="IVW29_001582"
     CDS             5330..5674
                     /locus_tag="IVW29_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7839064.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5687..5980
                     /gene="rpmA"
                     /locus_tag="IVW29_001583"
     CDS             5687..5980
                     /gene="rpmA"
                     /locus_tag="IVW29_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7839065.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6073..7221)
                     /locus_tag="IVW29_001584"
     CDS             complement(6073..7221)
                     /locus_tag="IVW29_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7839066.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7233..7544)
                     /locus_tag="IVW29_001585"
     CDS             complement(7233..7544)
                     /locus_tag="IVW29_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839067.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7597..7992)
                     /locus_tag="IVW29_001586"
     CDS             complement(7597..7992)
                     /locus_tag="IVW29_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7839068.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8028..8372)
                     /locus_tag="IVW29_001587"
     CDS             complement(8028..8372)
                     /locus_tag="IVW29_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839069.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8673..8894
                     /locus_tag="IVW29_001588"
     CDS             8673..8894
                     /locus_tag="IVW29_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839070.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9021..9797
                     /locus_tag="IVW29_001589"
     CDS             9021..9797
                     /locus_tag="IVW29_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7839071.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9794..9988
                     /locus_tag="IVW29_001590"
     CDS             9794..9988
                     /locus_tag="IVW29_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839072.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            9990..10274
                     /locus_tag="IVW29_001591"
     CDS             9990..10274
                     /locus_tag="IVW29_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839073.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10295..10483
                     /locus_tag="IVW29_001592"
     CDS             10295..10483
                     /locus_tag="IVW29_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839074.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10809..11048
                     /locus_tag="IVW29_001593"
     CDS             10809..11048
                     /locus_tag="IVW29_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839075.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11054..11740
                     /locus_tag="IVW29_001594"
     CDS             11054..11740
                     /locus_tag="IVW29_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839076.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11743..12573
                     /locus_tag="IVW29_001595"
     CDS             11743..12573
                     /locus_tag="IVW29_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7839077.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12590..13441
                     /locus_tag="IVW29_001596"
     CDS             12590..13441
                     /locus_tag="IVW29_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7839078.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13438..13797
                     /locus_tag="IVW29_001597"
     CDS             13438..13797
                     /locus_tag="IVW29_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7839079.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            13812..13973
                     /locus_tag="IVW29_001598"
     CDS             13812..13973
                     /locus_tag="IVW29_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7839080.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            13970..14275
                     /locus_tag="IVW29_001599"
     CDS             13970..14275
                     /locus_tag="IVW29_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839081.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            14275..14631
                     /locus_tag="IVW29_001600"
     CDS             14275..14631
                     /locus_tag="IVW29_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7839082.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            14591..14836
                     /locus_tag="IVW29_001601"
     CDS             14591..14836
                     /locus_tag="IVW29_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839083.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            14857..14946
                     /locus_tag="IVW29_001602"
                     /pseudo
     CDS             14857..14946
                     /locus_tag="IVW29_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            15021..15488
                     /locus_tag="IVW29_001603"
     CDS             15021..15488
                     /locus_tag="IVW29_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7839084.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            15763..15999
                     /locus_tag="IVW29_001604"
     CDS             15763..15999
                     /locus_tag="IVW29_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839085.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            16023..16364
                     /locus_tag="IVW29_001605"
     CDS             16023..16364
                     /locus_tag="IVW29_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839086.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(16357..16536)
                     /locus_tag="IVW29_001606"
     CDS             complement(16357..16536)
                     /locus_tag="IVW29_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP7839087.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            16758..17405
                     /locus_tag="IVW29_001607"
     CDS             16758..17405
                     /locus_tag="IVW29_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839088.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
BASE COUNT         5955 a         2765 c         3460 g         5290 t
ORIGIN      
        1 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
       61 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
      121 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
      181 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
      241 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
      301 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      361 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      421 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      481 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      541 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      601 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      661 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      721 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      781 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      841 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      901 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      961 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
     1021 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
     1081 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
     1141 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     1201 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1261 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1321 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1381 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1441 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1501 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     1561 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     1621 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     1681 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     1741 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     1801 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     1861 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     1921 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     1981 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     2041 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     2101 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     2161 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     2221 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     2281 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     2341 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     2401 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     2461 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     2521 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     2581 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
     2641 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
     2701 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
     2761 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
     2821 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
     2881 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
     2941 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
     3001 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
     3061 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
     3121 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
     3181 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
     3241 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
     3301 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
     3361 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
     3421 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
     3481 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
     3541 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
     3601 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
     3661 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
     3721 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
     3781 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
     3841 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
     3901 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
     3961 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
     4021 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
     4081 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
     4141 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
     4201 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
     4261 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
     4321 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
     4381 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
     4441 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
     4501 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
     4561 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
     4621 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
     4681 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
     4741 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
     4801 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
     4861 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
     4921 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
     4981 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
     5041 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
     5101 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
     5161 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
     5221 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
     5281 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
     5341 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
     5401 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
     5461 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
     5521 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
     5581 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
     5641 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
     5701 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
     5761 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
     5821 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
     5881 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
     5941 aaaacaagtg tctgtttatc cagtagctca agaagcttaa taaataaact gctctatctc
     6001 ttgtgtagca agggatggag cttttttgta aattatggga ttttttttga ccatactttt
     6061 gaccatactt tcttagaagt taatataatt tgttagtttt tgtgttgttt cttctttttt
     6121 gctgttagta atgtgtgtat atgtgtccat agtagttttt atatttgcgt ggcccattct
     6181 ttcttggact tctttgtgat ctgcgcctgc ctcatatagc attgatgcat gtgtatgtct
     6241 aaaaccatgc agaccgatat aaggtaagcc ggcacgctca cgatatatat tgtatctttc
     6301 tgccactgac tgattaattg tgaaattatt ttcgtcattg gtaaacaaat aattagcttt
     6361 agtaaatcct aattgaaata agtactttct ttgttcgagc ttccaaaatt ttagttgctt
     6421 tatagttttt tcatctaaat aaattattcg atttgatttt ttggttttag gagtttcaga
     6481 tatataatat ttatctgaac gagctacagt ttttttaatg gccatttcat ttgttttaaa
     6541 attaatattg tcccaagtga gtgctaatat ttcgccgatt ctacatccgc tgaatgctaa
     6601 tagtctaaag agcgtatagt ctctgttttt aaaatatgga ttctgttctt gagatacttc
     6661 atttagaaat aattccaact gttcttttgt atataatttt aatttctttt ctgttttaat
     6721 ttgtggcttt ggaataagta aatttaatgt tggattatca gatgttaaac caatatttat
     6781 agcatactta aaaactttat tcatatagtt tatgaaaaga gggtactgct ttgaagttcc
     6841 tttttcactc caatcattta ctaccttttg acaatatgcc gtattaattt tagaaagctt
     6901 aatatttcca aatttgggat atatatgttt attaaagatt attttagtcc ttgaatatga
     6961 actttctttt acagtttttt tgtagctttc tagccataat tctgctgctt ctttgaaagt
     7021 agtatttgta cttgtgggca ttcctgtttt ttgtaattct agttctaacc tagaaagtgc
     7081 tgtttttgct tctttttgtg ttctaaatcc tcgtttagta gtatattttt tctttccagt
     7141 tagcggatca ataccgagat aagttttaaa gtaccatgct ttttcgccat ttttcttttt
     7201 atattgtttt atcattgcca tcaatattac ccctattcaa attctaattc tgccatgcta
     7261 tcattgaaaa attcgatcag tccttgttcc tctataaatt caagccatat ttccttcccg
     7321 ttaattaatg gaactccttt ttctttacga attgtttgat acaagttagt cacgacttgt
     7381 ttccacattt ctaatacagc tttatctatt ttcttatcat cccagcgttt tttttggggt
     7441 ctatttttgt ttttttcaat ttgataatta gtagctttga tgataaatgt ttcgtatccg
     7501 gttgtgcttt tttctaacca ttctttgaat tctgtatata tcatttttgt tcacctttct
     7561 atttttgaac gtatgttctt ttttgcttaa aaaattttag agagctgcat aacaatagat
     7621 gcgagctttt aataattctt tcacaagtgg ttcgaagcga acgcttaaat tgttatcttc
     7681 tataaatttc atgtaattga tattgtgtgg ttctaattca gactttgcaa tatatctatc
     7741 taataatttt tcaatcatga agcgatcagc ctcatattcc atttttgaat ggagagagaa
     7801 tgctaagtta tataaaaaat aattatcatg atgtttagaa gcgtgtccta attcatgtaa
     7861 taaggttctt tgtcttctat acttagataa attagctttt attactatgg tattcataca
     7921 agcaacataa tggccatcgg catcaaggtt ttcacgctct tctaccttaa cgccgagttc
     7981 attaattatc atttcaattt gactgtccaa atgactcacc tacttattta gttatctttt
     8041 ttctctaaat atgcttcaat catagctgat agtatttccc tatcattatc ggtgagcggt
     8101 tttccgtcgc tactcataac agatgctaaa gcttcttcca cggttagttt tctttcctct
     8161 ggtgcaactc ccgcgttagg atcatctgtg cgtcctagca aataatctgt tgatacatgg
     8221 aagtagtcag caactttttg caaagtttct attttaggac tttgtgtctt ccatttataa
     8281 atagagtttt ttccaaatcc tattctattt tctaattcag aaatagaaat acctcttttt
     8341 ttacagagct ctttaaccct ttcgaaaagc atgtcaaatc aaccttccta gagcgtacga
     8401 taataattta tacaaaaaga tagaaaggta gttgacaaat ctatccaatt gtcttatatt
     8461 taactcgtaa gctaatttaa taagctaaca agcaacaaga aatacaccta ataaaataaa
     8521 cgaattcgag gtcggcaaac taagaatgtt aatttttagg gtttttctag gcttatttaa
     8581 ctgtatataa atataagaca gttggataga aatgtcaata aaaattagct aattttatta
     8641 gcttacatat ttaaagaaaa ggaggaaaca tcatgaaaca aaataaaaaa actccttccg
     8701 caaaaggagc tttaataact ttaaatttta aagaccaaaa gtttagtgct gttgttgatg
     8761 aaattcatcc acaagagcat caaaaaaatt cttttgcaac tgacgagaaa aagactcaga
     8821 aacgctctga gaaagttctt cgaataattc aatttctgtt ggaaaagatt cgtcattttg
     8881 gaagtctatt ttgaattctt tattaacagt cttgaatacg gtttgtttga ataaggcgtc
     8941 gacatccata ttaaattctt ccatactatt ttcacctcgc tttctattca aaattatatc
     9001 aaagaaagga atgagcaaaa atgaacacac cgcaaatttt taactttgaa caacatgaag
     9061 taagaacagt aacaatacat gatgaaccat tttttgttgg aaaagatgtg gctaaagttc
     9121 tagggtacca aaatggtagt agagatatta acagacacgt cgatgtagaa gatcggcaaa
     9181 actaccaaaa cggtactttt gaatcgccta gagggttaac tattattaac gaatcaggtt
     9241 tgtactcctt aattttagga agcaaacaac caaatgctaa gaaatttaaa cgttgggtga
     9301 ctagcgaagt gttgccagca attagaaaac atggaggtta tctaactcca gaaaaagtag
     9361 aagaagcttt gcttaatcca gatacaatca tccaattagc aactaagttg aaagaagaac
     9421 gtactggaag actaatcgca gaacaaaaaa ttgcagaata cgaacccaaa atttcgtatt
     9481 tggatagtat cttatcttct acagattcag taactattag tcaaatcgcg gctgattatg
     9541 ggatgtctcc acaacagatg aacaaactac ttcataagct aggtattcag aaaaaagtag
     9601 gcaaccaatg gttattatgc aaaaaacaca tgagacaggg ttacacaaaa tctcatacaa
     9661 ctgagattcc gaaatctgat ggcggaacaa aagttgtgat gaatacaaaa tggactcaaa
     9721 aagggcgtct gttcatttat gagtcgctga aaaaagaagg atatattcca gaaatcgatt
     9781 tattagagga ggaatgacga tgtcacaaga atttattttg aaagtcagaa ttcaattagc
     9841 aaaatacgga aagtctcaga actggttagc tgatactatc ggaatttcta gaccatatat
     9901 gtcggacatt atgaatgggc gtagaaaacc agataaacaa atcaagccaa ttgaggcagc
     9961 actagcagag ttagagaagg agaaataaca tgcaaataac aattccagat aatttagtag
    10021 tttccgaatt aactacacag attacgaatg ctgttcttaa ttcattggac gaacgattac
    10081 accttatgaa caaatcagta gagcttcctc catatccaaa caaatcagag gtaaaaaaag
    10141 ttttaggcat tggtgatgac aaattaacac attggataag cctaggctta aaaacacagc
    10201 agtggagcaa gttagacatc agaattgaac gatcggaact ccaaagattt ttgaaagaaa
    10261 actttgagtt ctaaaggcaa aggagaatga ttttatgtcc tacacattgc aacaagaaca
    10321 tcaaattcta ggtctgatta aacagcgtag aaaacaatta caagatgatc gtgcagcgct
    10381 tagaaaatcc gatgagctat cagatagaca agctgaacta attgcttctg aacttgagga
    10441 tttgagaatg ctagaaataa aaaataggga gattagatta tgaagaagac agacacactt
    10501 tttataggat tcattttggg gttattagtg attgtagcgc accaaagtat tatcggagga
    10561 agcttgttcg cagcattgat ggttttaatc aatctgcttg actcaaaaga aaggagcaac
    10621 tatggcacga gaagaagcgc taaaaatcgg taaagtgatt gctgataatt ggtgggcaaa
    10681 tagccgttct attattttaa gcaagcaaca tatcgaaaag aaaaaagcat ggcaacaaat
    10741 aaaaaagtga ctccgccgac caaagcaatg agtcactcaa aaaatcataa ctaaggagat
    10801 tttaacatat ggaaaatgaa ctttccactc tagatcaata tttggccgat cctgattggg
    10861 gcaaatcgaa tttacaactg gttgctaaca agtacgaaaa atcaattgat gaagcaattg
    10921 ttgcattgac tgatgcagct tccataaccg ctgatgacag taaatggacc aagagagact
    10981 tgggcattct aaaacgaggc gttaattggc ttgaagatca gtacagagaa gaaacaaaag
    11041 agaagtgata tgagtgttta aaccactaat agattcatac tcggcggtac taaagaaatt
    11101 taaaggtaac gacattggtg caacaatcaa cgaagaagta aatatcgaac ggctgaaaac
    11161 gatgtatgac ggatatgatg gcgatcgagt cattgaaatt cgctttattg atccacgacg
    11221 gttcacagta cagcaaagaa acttcatcta tgcgctcata ggcgatattt tcatcgatac
    11281 aggcatgcca acggacttct ggaaggaatt cttctacttc cgttttgaag gtgtcacagg
    11341 gcgcgaaata agcctcaaag acgaatcgaa tacaaccgtg agtgatgcca atatcttagc
    11401 gaatatcatc ctagatttca tctttgaaca tcatattcct ttcaaagaag gatatgagat
    11461 tttaccagcg aatcaagagt attacttcta caaatgcatt acaaaaagag tctgctgcat
    11521 ctgtggcaaa acaggagctg atatcgatca ctttgacaaa gcgctgggaa gacgaaagcg
    11581 caaagaagtt gatcattcag agtacacatt tgcagcactc tgcagaatcc atcacacaga
    11641 gaaacacaaa ataggtgtga tcaatttcaa aaataaatat caaatcaaag ggatcaagtt
    11701 aaaccaggaa acaattaaaa agttaaggat aggaggataa atttggctga gataagttgg
    11761 atcaaactta aaactactat gtttgacgat gaaaaaatac gattaatcca agctgttcct
    11821 gagtcggatg ccatcatcgt tatatggatt cgattactag ttttagcagg aaagactaac
    11881 gacgatggtc tgatatatat ccagaggaac atgccttata ccgaagaaat gcttgctaca
    11941 ttgtttggca aaaacgtaaa tacggttcgc ttagcgctaa ctacattggc aaatttcaac
    12001 atgattgatc taagcagtga tggactaatt gccatcagta attgggaaaa acatcaaaat
    12061 atcgagggta tggataaagt aaggctaaaa aatgctgaaa gaaaccgtaa atacagggaa
    12121 agaaagagac aggaacgtct caaattggaa aatgacgtta gcgtgacgtc acgtgacggt
    12181 acagataaag atatagaaga agataaagat atagataaag aagaaaagaa aggtaagtat
    12241 tctgacgaac acttacgcct tgctaaaaag ttgcaaagta atttaactga agattttcca
    12301 aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa
    12361 agagataaag catctataga agcgattgag tatgtgatca attggctacc tacaaatgaa
    12421 ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa
    12481 ttcgaaatca aagcagacaa gaagaatcat aaaaagcaaa gtcaaaaact acagtacagc
    12541 aatcctagtg aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct
    12601 agcaaatgct atggagaaac taataagaag agtattagtg caaagcggaa aatgtccaga
    12661 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
    12721 accaacatgc atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
    12781 acggatatat aacgatagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt
    12841 attaacagat aaaactttgt ttaaatgcaa aatggaaaat tatcacgtag tggatcaaga
    12901 aacaaaaatc gctttagaaa aagcaaaaag ctatacgaat gaggttctgc tgaaccatcc
    12961 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttat caatggccac
    13021 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
    13081 agaattatta gagcaaataa agttttctta taacaatact gaactgagaa aagaaattga
    13141 aggatcgctt atagccgata ttaaaacaac tgatttggtg gtttttgacg atattggagc
    13201 tgaattaggt agtggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct
    13261 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatctg gtcctgaact
    13321 aagagaagcc tacggtgaaa gaattgtttc taggatattt aagaattcag aaggttatgc
    13381 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
    13441 aataaatacc gtaataaaaa aactgttcat cgaggtatca agtttgattc tatcgcagaa
    13501 gcagagtatt acgatctagc cttgtggcaa gctgaagcaa atggctggaa agtaaaactt
    13561 caggaaagat ttgagctgat gccgaaattt gaactagacg gaaagaagta tcgcaagatc
    13621 gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc
    13681 aaaggaatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa
    13741 gcgccgttga tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaacga
    13801 ggtggtccat catgacaaca gaagaagtga ttcaaatgcg tattcgaaac attcagcgtg
    13861 aaattgacga tctagaacga acaaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
    13921 tcgatttgca catagagaac ttaagaaggg aaattcgtag attggaggaa tgagcgtgga
    13981 taagaaagcg acaatgaaac gaattgctga attaaccaag tcagaatctt ggcaggaaga
    14041 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcctaa
    14101 acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacaggtac
    14161 agctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctag
    14221 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat
    14281 ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaagac
    14341 aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac aggagcagga
    14401 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
    14461 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
    14521 catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga gtatttgagg
    14581 aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg atatcgaatc
    14641 aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg gaaagcgcca
    14701 gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg atttcgaaga
    14761 atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg aaaaatacga
    14821 ggaggacagc gaatgatacc gaagtttaga gcgtgggata gagaaaaaga cagaatgaag
    14881 gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat ttcacgtaca
    14941 aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa gtattatatc
    15001 ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag acaccaagcg
    15061 cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt tcagcatatc
    15121 tatcagctag atgtagagta gggcaacctc gagaaccaaa agtgacagca tcttattcct
    15181 tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg atcgataaag
    15241 aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc tattcagcga
    15301 tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc tatgatcgtt
    15361 atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc gaagaatcgg
    15421 ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa ctggttgctt
    15481 tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt ttccatttta
    15541 tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt gtcgaaagat
    15601 taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
    15661 agcttcgata ggcagcagcg gaaatattaa gaataaggat gtgaatttca actccttcta
    15721 aattgttctt attatctatc atccgttgct gtctattaat ttatgtattg gagggtatat
    15781 aaaatgaaga tcgcttccga aaaaattgat caactaacaa ttttagtaaa ccaaagaatc
    15841 gaagatatta tcaaagaatt cgaaggtgaa aagattaaga acataaagct tactttaatg
    15901 gatcataagt tcgaattaaa ttcgacaaaa aggaagatcg ttgagcttat agaagaagaa
    15961 atagctctcc agatcaatga gcaacaatta gctaattaat ttatgtattg gaggggaaat
    16021 gaatggaagt cgaacaaata aagtatcaag gtatggatcg ggaacggttg attgatatta
    16081 ttgagcaaca acgtttagaa ttgatccaaa aagaagctgt atgtaaagag tacaaaaaac
    16141 atttggaaca agtaattgag tatcattcag tagaaaagta cagatcagtg gtgcaaaaaa
    16201 atagagcaac gagtcctgat tctcgtcaag atgtatcgat tagtatcaag acgccagtat
    16261 ttaattctgt taaatcagaa aagctagaaa gtatctcaac tgctttggta gaaaaagcag
    16321 ctgaaatgaa ctccctagca aatgaagtta gtcgtcttat ttagtcatat cgataacttg
    16381 agagtctttg gtgattccgt tttttataga attaatggtt tgttcagcgg aatatttagt
    16441 caagtatgtt tcgctagttg ctactacttc attgttattg gacttaataa cgaagtaata
    16501 ttggccatta gttgcttctc ttataacaaa gtacaaattt ttcaccacct ataatttatt
    16561 tcagcggacc actcgctgat aattaaaatt ataagctatg tataaaattt cacaatatca
    16621 atttgtcgct gtggcggaaa gggtagacgc aataaaggat gaatgggaaa agtcaatgag
    16681 taattatctg aaatttacaa gtcccctgtt tagttatcta agattatttg ataatattat
    16741 ttctgaggtg atagaaaatg gtgggcgtag ttaaatttct tgggtttaca tttgatggat
    16801 atggtacgtt aggcgaatgg gtaggtatta ttttgatagt gattacaatt caacaaacat
    16861 caaagtacta ctggtattct aatagttcta aaattgtatc aagaataact gaaggaataa
    16921 ccaaaaaaga tattactgtc gtcggtaaat atgggtttac tataacttta ttaaatgaag
    16981 ggaaatctga aaccgcaata aagttttgcg ggattatagg taaatataac attttccaac
    17041 gttttatcag aaagcattgg tacaaatata tttacaaata ccctgtagtt aaaaagctta
    17101 ttgaaaaata tgtggaccac gaaatatctt atctcaatcc aacagacctt attaatcaag
    17161 aattccagaa tattggatac ttagaaaaag gtctaaaaat accaattgta aatgaaagtt
    17221 tagttactgc aatggtagct aacataaaga acagcaagaa attagaaaag agatataaaa
    17281 aatgtaaaaa attgaaattt tcctttttat tcttagaata taatggcaaa gaatattatc
    17341 aagatattta tgttttgcga ggctcgaact tatataaaga cattaaaaat aagatttcag
    17401 tatgagattt tttaaaattc gtagatcact ctttgagtgg tctttttatt ttgcgtaaag
    17461 gaggcacgac
//
LOCUS       DACCVU010000053        17408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCVU010000053 DACCVU010000000
VERSION     DACCVU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.053"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(172..717)
                     /locus_tag="IVW29_001608"
     CDS             complement(172..717)
                     /locus_tag="IVW29_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7839089.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(729..1421)
                     /locus_tag="IVW29_001609"
     CDS             complement(729..1421)
                     /locus_tag="IVW29_001609"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7839090.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1440..1724)
                     /locus_tag="IVW29_001610"
     CDS             complement(1440..1724)
                     /locus_tag="IVW29_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839091.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1737..2300)
                     /locus_tag="IVW29_001611"
     CDS             complement(1737..2300)
                     /locus_tag="IVW29_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7839092.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2528..3190
                     /locus_tag="IVW29_001612"
     CDS             2528..3190
                     /locus_tag="IVW29_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7839093.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3314..4510
                     /locus_tag="IVW29_001613"
     CDS             3314..4510
                     /locus_tag="IVW29_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7839094.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4611..5987)
                     /locus_tag="IVW29_001614"
     CDS             complement(4611..5987)
                     /locus_tag="IVW29_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7839095.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6024..6890)
                     /locus_tag="IVW29_001615"
     CDS             complement(6024..6890)
                     /locus_tag="IVW29_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7839096.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6979..8004)
                     /locus_tag="IVW29_001616"
     CDS             complement(6979..8004)
                     /locus_tag="IVW29_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7839097.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8022..8696)
                     /locus_tag="IVW29_001617"
     CDS             complement(8022..8696)
                     /locus_tag="IVW29_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839098.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8880..9773)
                     /locus_tag="IVW29_001618"
     CDS             complement(8880..9773)
                     /locus_tag="IVW29_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7839099.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9847..11544)
                     /locus_tag="IVW29_001619"
     CDS             complement(9847..11544)
                     /locus_tag="IVW29_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7839100.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11537..12850)
                     /locus_tag="IVW29_001620"
     CDS             complement(11537..12850)
                     /locus_tag="IVW29_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7839101.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12850..16887)
                     /locus_tag="IVW29_001621"
     CDS             complement(12850..16887)
                     /locus_tag="IVW29_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7839102.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16900..17358)
                     /locus_tag="IVW29_001622"
     CDS             complement(16900..17358)
                     /locus_tag="IVW29_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7839103.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4894 a         3897 c         3028 g         5589 t
ORIGIN      
        1 ataaaaagaa aaaatataac ttaagttgtt gataataaag agatttatta acggaaaaag
       61 aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt ttgttttatg
      121 ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt tttataaaat
      181 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
      241 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
      301 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
      361 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
      421 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
      481 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
      541 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
      601 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
      661 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
      721 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
      781 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
      841 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
      901 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
      961 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
     1021 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
     1081 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
     1141 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
     1201 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
     1261 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
     1321 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
     1381 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
     1441 tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat aataagagat
     1501 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
     1561 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
     1621 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
     1681 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
     1741 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
     1801 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
     1861 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
     1921 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
     1981 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
     2041 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
     2101 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
     2161 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
     2221 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
     2281 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
     2341 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
     2401 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
     2461 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
     2521 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
     2581 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
     2641 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
     2701 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
     2761 ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca
     2821 gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag cagcgaaagc
     2881 tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc agtttcttta
     2941 tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca atgaccatga
     3001 gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca tcgaccagct
     3061 gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag atgatatcga
     3121 cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg aaccagaaca
     3181 aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga tgtctggctg
     3241 tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca gattcaacaa
     3301 ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac cagttaacga
     3361 tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc tgaccacttc
     3421 acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt taattgacaa
     3481 actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg atgctaatga
     3541 tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg aattctcaca
     3601 atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt ccttaactga
     3661 actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag aagggaacat
     3721 cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag caaaatatca
     3781 aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa aagacggtct
     3841 gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact actttgatgc
     3901 actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa caacgatcat
     3961 tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc agattgccaa
     4021 tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc gcttagctag
     4081 acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa atcaggcctt
     4141 ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa accaagtcgt
     4201 gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga
     4261 aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag ccatcgaaac
     4321 agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga
     4381 tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg
     4441 tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac tgttggattt
     4501 aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca gtttattaca
     4561 actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt ctatttctca
     4621 ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat aaccattacg
     4681 ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat acaagtcaag
     4741 atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc ttttggtttg
     4801 ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata ggcgagcgcc
     4861 atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa gaatacagct
     4921 gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc gatttctgtc
     4981 ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc aagtgaatac
     5041 atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat caccgcgccc
     5101 actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac tcctagttca
     5161 gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac agtagcaatc
     5221 agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac ttcctcggct
     5281 gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc accatctagc
     5341 acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac gaaaaatatt
     5401 cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc attattcaaa
     5461 cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac tacgagcacc
     5521 gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa tgcttgtgga
     5581 agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc ggaagcaaca
     5641 gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat
     5701 tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac tcgcgaagca
     5761 aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac aaatgaaagt
     5821 gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa tattcccgta
     5881 ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc ttttttcatc
     5941 tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga agttcctccc
     6001 cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag gataccacct
     6061 aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg
     6121 ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag
     6181 ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat
     6241 aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca
     6301 aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc
     6361 aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat
     6421 gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca
     6481 tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat
     6541 acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa
     6601 attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca
     6661 tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa
     6721 atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca
     6781 ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa
     6841 ccaatactac cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc
     6901 cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt
     6961 ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa
     7021 agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc
     7081 ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc
     7141 atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat
     7201 ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat
     7261 gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga agataaaagc
     7321 gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt
     7381 tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc
     7441 atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg
     7501 ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc
     7561 aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc
     7621 atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat
     7681 tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc
     7741 tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt
     7801 tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc
     7861 ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca
     7921 catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc
     7981 tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc
     8041 tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac
     8101 cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct
     8161 cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga
     8221 cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca
     8281 acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa
     8341 gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc
     8401 acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg
     8461 gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca
     8521 tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct
     8581 gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc
     8641 tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa
     8701 aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg
     8761 gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa
     8821 tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat
     8881 tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg
     8941 aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat
     9001 ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc
     9061 actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc
     9121 tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg
     9181 acttcataag cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt
     9241 tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa
     9301 tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct
     9361 aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag
     9421 atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc
     9481 gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc
     9541 gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt
     9601 tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct
     9661 gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat
     9721 tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc
     9781 accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt
     9841 tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat
     9901 caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga
     9961 cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat
    10021 ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg
    10081 aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc
    10141 ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc
    10201 ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa
    10261 tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc
    10321 actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg
    10381 ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt
    10441 gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa
    10501 atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc
    10561 ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc
    10621 ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc
    10681 aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac
    10741 agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc
    10801 tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct
    10861 aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata
    10921 caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac
    10981 catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg
    11041 agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc
    11101 cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat
    11161 acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat
    11221 cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa
    11281 atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc
    11341 accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc
    11401 ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac
    11461 tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc
    11521 cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag
    11581 cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg
    11641 ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt
    11701 tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag
    11761 agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt
    11821 gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg
    11881 cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag
    11941 gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat
    12001 ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag
    12061 caggagccat atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag
    12121 caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt
    12181 atggatctct tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg
    12241 ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga
    12301 tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat
    12361 cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa
    12421 ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta
    12481 gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca
    12541 tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg
    12601 ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga
    12661 tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca
    12721 tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat
    12781 gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca
    12841 gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc
    12901 tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc
    12961 tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt
    13021 tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt
    13081 tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc
    13141 attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt
    13201 tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga
    13261 agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata
    13321 ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt
    13381 catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac
    13441 ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat
    13501 gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc
    13561 agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag
    13621 atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata
    13681 gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta
    13741 tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc
    13801 taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt
    13861 gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg
    13921 ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg
    13981 caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat
    14041 gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg
    14101 ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc
    14161 tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc
    14221 tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc
    14281 tgcgccattg attggcatat tactgccttc aattagttta ttgatattca tgtggattgg
    14341 cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat
    14401 gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc
    14461 tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg
    14521 acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc
    14581 tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc
    14641 tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt
    14701 agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc
    14761 aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg
    14821 gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc
    14881 attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc
    14941 gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc
    15001 taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt
    15061 cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg
    15121 gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc
    15181 agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt
    15241 tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc
    15301 attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt
    15361 tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat
    15421 ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac
    15481 tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga
    15541 agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg
    15601 atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg
    15661 agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc
    15721 attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct
    15781 cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac
    15841 gattaggatt ttttctggat ttctattttc tccgccattg tctttataca atgtttcata
    15901 gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt
    15961 attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat
    16021 ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat
    16081 atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc
    16141 agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc
    16201 tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag
    16261 atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg
    16321 cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc
    16381 aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc
    16441 gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt
    16501 tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg
    16561 atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa
    16621 cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg
    16681 gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat
    16741 gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc
    16801 ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg
    16861 gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg
    16921 gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc
    16981 gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata
    17041 ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc
    17101 gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca
    17161 ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa
    17221 gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg
    17281 atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt
    17341 caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac
    17401 aatcaaca
//
LOCUS       DACCVU010000054        17356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCVU010000054 DACCVU010000000
VERSION     DACCVU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.054"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(40..2490)
                     /gene="parC"
                     /locus_tag="IVW29_001623"
     CDS             complement(40..2490)
                     /gene="parC"
                     /locus_tag="IVW29_001623"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7839104.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(2502..4547)
                     /gene="parE"
                     /locus_tag="IVW29_001624"
     CDS             complement(2502..4547)
                     /gene="parE"
                     /locus_tag="IVW29_001624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7839105.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4713..5147)
                     /locus_tag="IVW29_001625"
     CDS             complement(4713..5147)
                     /locus_tag="IVW29_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7839106.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5274..5912
                     /gene="plsY"
                     /locus_tag="IVW29_001626"
     CDS             5274..5912
                     /gene="plsY"
                     /locus_tag="IVW29_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7839107.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(6044..6919)
                     /locus_tag="IVW29_001627"
     CDS             complement(6044..6919)
                     /locus_tag="IVW29_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7839108.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(7001..7792)
                     /gene="codY"
                     /locus_tag="IVW29_001628"
     CDS             complement(7001..7792)
                     /gene="codY"
                     /locus_tag="IVW29_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7839109.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7810..9210)
                     /gene="hslU"
                     /locus_tag="IVW29_001629"
     CDS             complement(7810..9210)
                     /gene="hslU"
                     /locus_tag="IVW29_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7839110.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9224..9772)
                     /gene="hslV"
                     /locus_tag="IVW29_001630"
     CDS             complement(9224..9772)
                     /gene="hslV"
                     /locus_tag="IVW29_001630"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7839111.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9809..10714)
                     /gene="xerC"
                     /locus_tag="IVW29_001631"
     CDS             complement(9809..10714)
                     /gene="xerC"
                     /locus_tag="IVW29_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7839112.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10912..12288)
                     /gene="trmFO"
                     /locus_tag="IVW29_001632"
     CDS             complement(10912..12288)
                     /gene="trmFO"
                     /locus_tag="IVW29_001632"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7839113.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12257..14335)
                     /gene="topA"
                     /locus_tag="IVW29_001633"
     CDS             complement(12257..14335)
                     /gene="topA"
                     /locus_tag="IVW29_001633"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7839114.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14457..15314)
                     /gene="dprA"
                     /locus_tag="IVW29_001634"
     CDS             complement(14457..15314)
                     /gene="dprA"
                     /locus_tag="IVW29_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7839115.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15370..16137)
                     /locus_tag="IVW29_001635"
     CDS             complement(15370..16137)
                     /locus_tag="IVW29_001635"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7839116.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16130..16990)
                     /gene="ylqF"
                     /locus_tag="IVW29_001636"
     CDS             complement(16130..16990)
                     /gene="ylqF"
                     /locus_tag="IVW29_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7839117.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4447 a         3848 c         3015 g         6046 t
ORIGIN      
        1 taatacagat ggcgtctctt cattaaaata ctacaacagt tacgactcga tgaaaatctc
       61 aggagcatcg ataacttcga tgacttctcc accttttttc tcatccatcg caaatgatcc
      121 attcgatgtt cgatcaccta tagggaaatc agttgggtca atcgttaact gctgcccttt
      181 ttgattcatc aaggttattt cttttgatct tccttccgac atatagataa tacggtgcgg
      241 attttttttc agctcacgca tgaccgtcac tccccgcttc gcacgtgaag tttggctgat
      301 ttcttgagca agcatgcgtt taacagctcc tcgctgagta acgattacga tcggcgtatc
      361 accttctgct aacacaagca gcccgttgat aatcctatct ccatctttca aattcataga
      421 cttcactcca gctgcctttg ctccaacaac gggaacatct gttaaaggat agcgcaaacc
      481 cattccttga tgcgttacta agaaaacatc taagcctggc tgatcttctt tcagatacac
      541 agcaatcagt tcatcttctt ccgcctttag cttcatacag ctgagcggac ggctcttata
      601 ggtgcgccat ggttcgaact ctgtcattcg tgtttgcttg atcaatccat tcttgctgat
      661 gaatacaaat gttttctctg cttgaatttt ttgatatggg aaaacagcta aaatcgattc
      721 gtctacagac agattggaga ttgtttgaga aatgtgctca ccagcatctt tccatttcaa
      781 atcgggtagt tcatggacag ggcggtagat cacatttgct ttatttgtga tcagaagaac
      841 atggtccaat gtactcaatt cacctgagta tagcaagtag tcgccttctc gcatcccgat
      901 ttcttctggt ttggaagcac tataagaacg aaggcttgtt cgcttcacat agccttctcg
      961 cgtcacactg acaattacgt cttcttgtgc cactaagact tgcgtatcaa ttttgatttc
     1021 ttcgatttcg tcttcgattg tcgttagacg agcggaagaa taattttttt tcacttctcg
     1081 caattctttt ttcataacag agaaaagctc tttgtcattt gaaaggattt tattcaactc
     1141 tgtgatttgc gcaatcaagc tttcggattc cttttgaagc tctgtgatat ccgtgttcgt
     1201 caaacggtat aattgcaatg tgacaattgc ttctgcttgt tcttctgtaa attgaaagac
     1261 gtcgactaaa ttctttttgg cgtctttttt gtctttactt tcacgaatcg ttgcgattac
     1321 ttcatccaga atcgataaag ctttcatcaa tccttctacg atatgctgtc ttttttgagc
     1381 tttggctaaa tcgaattgac tacgttttgt tatgacttgc tttcgatgct cgatataact
     1441 tcttaaaatg tctttcagtc caacttgatg cggagtcata tggtcgatag cgaccatatt
     1501 gaaattgtaa ttgatctgta gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc
     1561 attggcgtct tttttcaatt cgacgacaat ttgcagacct gttctatcac tttcatctcg
     1621 tacttcagct attccatcga ttttcttatt caaacggatc tcatccattt ttttcaccaa
     1681 ggtcgcttta tttacttcat atgggatttc tgtgatcacg atttgctgtt tattaccctt
     1741 cattgcctct atctttgttt tcgaacgaag aataactttt cctcgtcctg tttcataagc
     1801 ttttttgatt tcatcttttc cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat
     1861 atattccatc aatttttcaa ggctcgcttg tggatgatcg atcatataga ttgtgccatc
     1921 gatcacttca gctaagttat gagtcgggat ttctgttgca tatccagcag atatacctgt
     1981 cgatccattg acgagaaggt ttgggtatcg agctggaagg actgttggtt ctttttctgt
     2041 atcatcaaaa ttccacacga gatcgactgt ttctttctcg atatctgcta gcatctctcc
     2101 acttaacttg gataatctgg cttcagtata acgcatcgca gcaggtggat caccgtccat
     2161 actcccgtta ttcccgtgca tttcgatcag tacttcccgc aacttccagt cttggctcat
     2221 gcggaccatc gcttcataaa tactgatgtc accatgagga tgataattcc ccatgatatt
     2281 tccgacagat tttgctgatt tacggaatcc tttgtcatac gtattgccat ctttgttcat
     2341 ggcaaataat atccggcgtt gcactggctt caatccatca cggatatcag gcagtgcacg
     2401 ttcttggata atatatttgg aataacgtcc gaagcggtca cccatcactt cttctaaagt
     2461 taattcttgt acttcttgtc gattttccat caggtgacct cctattctac ttcaaataac
     2521 tgattgtttt catttttatc tgctcgttct tcatctaata aatcattaga aactgaagga
     2581 gagatttcag tatcctcttt tttatctaag atactgccat cttcttcgag cgtaaattgc
     2641 acgtgatttt cgatccattt tcttcgaggt tctactttgt cacccatcag tgtggttact
     2701 cgacgttcag cttgcgcagc atcatcgata cggacgcgga tcaacgtacg ggaagtcgga
     2761 tccatcgttg tttcccacaa ttgttccgca ttcatctctc caagtccttt atatcgctgc
     2821 agcatatatc ctttgccgac ttttttaatc attgctgcca attcgtcatc tgtccacgca
     2881 tattcaatga cttgcttctt tccttgtcct ttggatactt tatataaagg cggtaacgcg
     2941 atatatactt tcccagcttc aatcagcggt ttcatataac gataaaagaa agtaagcagt
     3001 aatacttgaa tatgggcgcc gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa
     3061 ttacagtctt cgatagaaaa ctcaggaccg actcctgcac caatcgtata gatcatcgta
     3121 ttgatttctt catttttcaa gatatcttgc attttagctt tttcggtatt gatgactttt
     3181 cctcgtaaag gtagaattgc ttggaacttt ctgtctcttc cttgtttagc agatccgcca
     3241 gcagaatccc cttcgactaa atacaattca tttttcttag ggtttcggga ttgtgcgggt
     3301 gtcaattttc ctgaaagaag agattcgcct tttttacgct tcttcccatt tcggctttct
     3361 tctctggctt tacgtgctgc ttctctcgct tctctggctt tgattgcttt acggaccaac
     3421 atctggctca tttcgctatt ttcctgcaaa taaaagccca tttgttcact gatgacgtta
     3481 tctactacgg tacgagcaac tggcgtacca agtttttctt ttgtttgccc ttcaaattgc
     3541 agaagatttt caggtacacg gatagaaagt acagcagcta acccttctct gaaatcacta
     3601 ccttccagat tcttgtcccg ttctttcagc aaaccaactt ttctcgcata ttcattataa
     3661 gatttcgtca tcgccgcttt cataccagct tcatgggttc ccccatcttt tgtccgaacg
     3721 ttattgacaa aagataaaac attttctgaa taaccgtcat tgtattgata agcgacctct
     3781 acttcgatac cttctttttc tcctgaaaaa taaacaactg gtgtcaatgt atctttttct
     3841 tcattcagat aatcaacaaa ttctttgatt ccttcttcgt aatgaaaaat ttccttgact
     3901 ggttcttctc cacgaagatc agaaagagag attttaacgc cttttagaag aaaggcagat
     3961 tctcttaacc gttcggctaa aatttcatat gaaaatttcg ttgtactgaa tattgtatca
     4021 tctggtaaaa aatggacagt cgtcccgttt gctttttttg tttttcctat ctttttcaaa
     4081 gtaccgtcag gtttaccgcc gtttttaaat ttctgctgat actctacgcc atctctaacg
     4141 atcgttacaa ttagccattt cgataaggca tttaccacac tggctcctac accgtgcagt
     4201 ccaccagaag ttttgtaccc tccttgacca aactttccgc cagcatgcag cacagtaaaa
     4261 atcacttcaa ctgttggtat tccggaagca tgcattccta caggcatccc acgtccgcta
     4321 tctgcaacag ttatactatt gtcttcatgg atggtcacat caatctcact gccataacct
     4381 gataatgctt cgtcaactgc attatcgacg atttcataga ctaaatgatg caagcctcgg
     4441 ctgtcagtgg atccaatgta catccctggt ctttttctaa ctgcctctaa tccttccaat
     4501 acttggatgg acgagtcatt gtattcgttg tttacttttt ttgccaaggc aaacgctcct
     4561 tcaataatac tttgcggcaa aaccgcacca cgattatcat actagaaaag gtcgtgaaaa
     4621 aaaagagggt agacgagaaa tcaattttaa ttatctccta accgcttttt cgacattccc
     4681 ctctatcgat catctcttct ttgtggtcat ttttattttt ggttttcttt caacttacca
     4741 agttcaatct taatacaacg atccattacg atatctgtac gtcctgcttt tcttagcatc
     4801 tcagctgctt ctttgttttc aatccctaat tgtgcccaga acactttagc atcagttttc
     4861 aaaaaatcac ctgctatttc aggtaaaaat tcactccgtc taaatacatc tacgatatcg
     4921 attggatcat cgatttctgt caaattcgca tagactttct cgcctagaat ttcttcacca
     4981 gccaataatg gatttactgg tatgatttta tatccattgt cttgcaatac ctgtgcaacc
     5041 atgtaacttg ttcgttctgg tttattgctc aagcctacga cggcaatatt ttttgcttct
     5101 tccaaatagt tgaaaatcgt ttcttgtgtt ggatttttta cagacatttc tgccacccct
     5161 tgcttttctt tttattccat ctattatact tgtatctttc attttttcaa ttgttcctgt
     5221 tacctattca tgctaaaata aaggatagtt ttcaacttga aagagggtaa aaaatgaaat
     5281 tgatcttatt acttatttta gcatatcttt taggttctat tccttccggt gtctggattg
     5341 gcaaattgtt tttcaaaaaa gatatccgac aacacgggag cggtaatact ggaacgacca
     5401 atacatttcg tgtattaggc aaaacagccg ggattgttgt cctgcttatg gatattttaa
     5461 aaggtacatt ggctacttgt ttgccgatga tcttccacct ttctgggatt aaccctcttt
     5521 ggtttggggt atgcgccatt cttggccata catttccgat ttttgctggg ttcaaaggag
     5581 gaaaagctgt tgcgacaagt gcaggaatgc ttttaggatt taatcctgta ttttttgttt
     5641 attcttgtat catctttatc gttagtttat tttgtacaag tatggtgagt ttgagcagta
     5701 tgatcagtgc agtattgatc actttatcta cgatcgtcct tccttatgta gctcctgtga
     5761 ttttggctaa gcccaattgg ttactgacga tcattgcctt tttagtttct tcctttatct
     5821 tttatcggca taaagacaat atcaagcgta tcagaaacca tactgaaagt cgaatcccgt
     5881 ttggtttgaa tagcagcaag aaaaaatcct gatacttttt cattccaaag agataaaacg
     5941 ggagtgtgac aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac
     6001 cttggtaaac ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg
     6061 aatattttgt cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca
     6121 agtttccact actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag
     6181 gcgcttcttt tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag
     6241 ttacgctatg ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa
     6301 aaaccaaggc atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac
     6361 ctaacgtctt ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt
     6421 tcaatggaga aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat
     6481 tgaaagcagg atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt
     6541 gataatgaac cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa
     6601 atggatactt ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc
     6661 catgttcaat gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg
     6721 ttttcccttg ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg
     6781 gtgcatgtct gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt
     6841 ttgatttcag actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat
     6901 tttgaataat aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag
     6961 gctggaacaa acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta
     7021 actcgtcttt aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac
     7081 gagattcaat gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag
     7141 tgatgccgat actatcggca atattagaag cagtcagacg accttcatct ccatccaatg
     7201 cttcaaagat agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt
     7261 ggacagccgt tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct
     7321 gcataccgac aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt
     7381 gttccatacg agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga
     7441 cagtcgtcag accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac
     7501 tagtaattgg gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt
     7561 caggaaattg tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt
     7621 cattataacc tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac
     7681 cacctagtgt gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct
     7741 tttgctgcaa aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc
     7801 tccgattttt tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt
     7861 gtcatccaca tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa
     7921 taatagatct tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc
     7981 tgtgtcacgg ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa
     8041 cgtaactgac acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt
     8101 tggttcggtc agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat
     8161 cgggaagcgt ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc
     8221 tgaagcaatg aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga
     8281 accttcgaca attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg
     8341 ctgtgattta gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc
     8401 taatttgatt gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt
     8461 gatcaataac tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt
     8521 caaagcacct agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat
     8581 tggtgcaggt ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc
     8641 cttttgtagt tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc
     8701 ttgcggttca gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg
     8761 attagtggat tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt
     8821 ttctgcattt tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg
     8881 gatcgcattt tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac
     8941 ttcagtaaat tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg
     9001 tcgtgcgatt tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt
     9061 aatatcttgt tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac
     9121 ggcaacagat ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat
     9181 ttgtcttggt gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga
     9241 taatattatg attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa
     9301 tttctttcgc actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg
     9361 caaaattccc tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg
     9421 tacctgaaac aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt
     9481 gcatagattg ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc
     9541 cgttatattc gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac
     9601 ttccggcgaa acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc
     9661 ctttcatgac aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc
     9721 cgtctttttc gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt
     9781 cctcctaaat ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt
     9841 tttgtaaact ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc
     9901 ctagcaattc ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg
     9961 tatggcgcag catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat
    10021 tcaagacata ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga
    10081 tatcatgctc ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt
    10141 gcagagcatc ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat
    10201 ggatcaacat gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac
    10261 ttaaacggat gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag
    10321 gactatctcc tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg
    10381 gcagtctcaa atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt
    10441 cttctttgag aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat
    10501 ttctgctata gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat
    10561 attccgcctt cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa
    10621 tgtcttcttc atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac
    10681 gcaaaaattt ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag
    10741 aataatttag cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt
    10801 ctaagtttgt tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa
    10861 acgcaagaaa aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt
    10921 tgggattgca gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg
    10981 tgcaattgct tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag
    11041 accatagttg gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc
    11101 catacttcca atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg
    11161 tgcggcgttg attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc
    11221 agtcatctga cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag
    11281 caattctggc gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc
    11341 attttctaac ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt
    11401 ctgaaagccc acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac
    11461 agcataagga cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa
    11521 aagcatcgtt ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata
    11581 tttttctttc tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa
    11641 agctttgaat tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata
    11701 gcgggacttc aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc
    11761 tgcatcatag aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg
    11821 atgagaagtc aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc
    11881 ttcgtttatc actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa
    11941 agaatcgcgg tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt
    12001 gattactacc gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa
    12061 actgtttcct cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc
    12121 ctcagtcgat ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat
    12181 ctgccaagca gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga
    12241 aagaacctct tttctattat ttttggatgt cttcttcgta atctccattt acacagacta
    12301 cttgtttacc gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc
    12361 tgcccactgg tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga
    12421 atagtctgtt tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag
    12481 tcacaccgat ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag
    12541 cataaaactt cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat
    12601 cgaatcctgc tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca
    12661 gttctttttc aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt
    12721 cttcaatttt atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag
    12781 ggaagaattc acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt
    12841 tttgtgtcaa ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg
    12901 aaggtcgccc taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg
    12961 ctggtggttg agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt
    13021 cttcaagttc tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat
    13081 acacttgcat gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat
    13141 tttgttctaa tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa
    13201 aacgtgacca gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt
    13261 catctggtgt acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt
    13321 gagcattttt gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa
    13381 taaatgctgc ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt
    13441 atgtaatcaa gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg
    13501 ctaccatcat ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc
    13561 tacttgtagt gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa
    13621 cttcgaagtc tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta
    13681 atttcttttt cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt
    13741 taaaattacc gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac
    13801 ggtcaataat aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga
    13861 ccttacgcca gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag
    13921 cttgctgtgc atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga
    13981 ctgcgtcttt tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa
    14041 gtaggtacgc taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt
    14101 aaactttttg agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc
    14161 gtatagaaat ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg
    14221 gtaaatctct tatatggcca acactggcca ttactttata gtttcgtcca agatatttct
    14281 caattgtctt ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg
    14341 aatggactcc tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag
    14401 ctaatcatat ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat
    14461 tctttaaatt ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc
    14521 aaatgatgac atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt
    14581 cttccaagat ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca
    14641 aagacacatg ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc
    14701 tttttcgggc cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc
    14761 tcttgcaagt cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg
    14821 attgtccgtc caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg
    14881 cagccactga cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt
    14941 tttttggcta aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt
    15001 aaagatagat caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga
    15061 gggggatatt tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag
    15121 ttagcgtgaa tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct
    15181 atcttaatga tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca
    15241 agtgcccatt ttccttgact actgattcct tttgtataag caagtttcaa taaattttct
    15301 tctatttgat acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc
    15361 ggctgttttt tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt
    15421 aattccttgt cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga
    15481 gaaaccatat ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt
    15541 agcaatgata cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc
    15601 ctgctgaata ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga
    15661 caatttttct actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc
    15721 gattgtctgc tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg
    15781 ataaagaagt tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat
    15841 ctcatttttc ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc
    15901 tacttcatca atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca
    15961 aaacatttct tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga
    16021 agccagtgct tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc
    16081 atctattctc tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac
    16141 ttttttgctc catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa
    16201 tatcatgcac gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg
    16261 taatatccat cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac
    16321 gatcgctgat tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat
    16381 catctaaatg gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt
    16441 cttcatcttc aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac
    16501 ctaaacgaag ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt
    16561 tcttgcctac tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca
    16621 ttgcccgaat agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca
    16681 atgcttcttt agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag
    16741 ctaatgcaag atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat
    16801 ctgccagatc cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta
    16861 gcattggatt acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca
    16921 catatttcat tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt
    16981 ggatggtcat tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt
    17041 ttttcttttt caatataaat tcactttttt attataaaga aattccatct attagttaag
    17101 tgaaagtgaa ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta
    17161 ttttggtttc tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag
    17221 acgaacaaaa cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg
    17281 ttgctgatgt caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag
    17341 aattttctac tgaaga
//
LOCUS       DACCVU010000055        16610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCVU010000055 DACCVU010000000
VERSION     DACCVU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.055"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(54..788)
                     /locus_tag="IVW29_001637"
     CDS             complement(54..788)
                     /locus_tag="IVW29_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7839118.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IVW29_001638"
     CDS             1115..1525
                     /locus_tag="IVW29_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7839119.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IVW29_001639"
     CDS             1518..2219
                     /locus_tag="IVW29_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7839120.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IVW29_001640"
     CDS             2219..3805
                     /locus_tag="IVW29_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7839121.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IVW29_001641"
     CDS             3824..4045
                     /locus_tag="IVW29_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7839122.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IVW29_001642"
     CDS             4042..4803
                     /locus_tag="IVW29_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7839123.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IVW29_001643"
     CDS             5183..5692
                     /locus_tag="IVW29_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7839124.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IVW29_001644"
     CDS             complement(5767..6306)
                     /locus_tag="IVW29_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7839125.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IVW29_001645"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IVW29_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7839126.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IVW29_001646"
     CDS             7373..8170
                     /locus_tag="IVW29_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7839127.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IVW29_001647"
     CDS             8198..8833
                     /locus_tag="IVW29_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7839128.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IVW29_001648"
     CDS             8853..9626
                     /locus_tag="IVW29_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7839129.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IVW29_001649"
     CDS             10034..10831
                     /locus_tag="IVW29_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7839130.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IVW29_001650"
     CDS             10809..11222
                     /locus_tag="IVW29_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839131.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IVW29_001651"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IVW29_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7839132.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IVW29_001652"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IVW29_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7839133.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IVW29_001653"
     CDS             15999..16559
                     /locus_tag="IVW29_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7839134.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5403 a         2967 c         3596 g         4644 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt
//
LOCUS       DACCVU010000056        16188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCVU010000056 DACCVU010000000
VERSION     DACCVU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.056"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(49..546)
                     /locus_tag="IVW29_001654"
     CDS             complement(49..546)
                     /locus_tag="IVW29_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7839135.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(676..1386)
                     /gene="budA"
                     /locus_tag="IVW29_001655"
     CDS             complement(676..1386)
                     /gene="budA"
                     /locus_tag="IVW29_001655"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7839136.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1399..3063)
                     /gene="alsS"
                     /locus_tag="IVW29_001656"
     CDS             complement(1399..3063)
                     /gene="alsS"
                     /locus_tag="IVW29_001656"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7839137.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3268..3930)
                     /locus_tag="IVW29_001657"
     CDS             complement(3268..3930)
                     /locus_tag="IVW29_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7839138.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3940..4755)
                     /locus_tag="IVW29_001658"
     CDS             complement(3940..4755)
                     /locus_tag="IVW29_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7839139.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5017..5460)
                     /locus_tag="IVW29_001659"
     CDS             complement(5017..5460)
                     /locus_tag="IVW29_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7839140.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5594..5932)
                     /locus_tag="IVW29_001660"
     CDS             complement(5594..5932)
                     /locus_tag="IVW29_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7839141.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5920..6297)
                     /locus_tag="IVW29_001661"
     CDS             complement(5920..6297)
                     /locus_tag="IVW29_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7839142.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6521..7714
                     /locus_tag="IVW29_001662"
     CDS             6521..7714
                     /locus_tag="IVW29_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839143.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7880..8302)
                     /locus_tag="IVW29_001663"
     CDS             complement(7880..8302)
                     /locus_tag="IVW29_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7839144.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8697..8891
                     /locus_tag="IVW29_001664"
     CDS             8697..8891
                     /locus_tag="IVW29_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839145.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8888..9382
                     /locus_tag="IVW29_001665"
     CDS             8888..9382
                     /locus_tag="IVW29_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839146.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9544..10716)
                     /locus_tag="IVW29_001666"
     CDS             complement(9544..10716)
                     /locus_tag="IVW29_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7839147.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10922..11737)
                     /locus_tag="IVW29_001667"
     CDS             complement(10922..11737)
                     /locus_tag="IVW29_001667"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7839148.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11887..12333)
                     /locus_tag="IVW29_001668"
     CDS             complement(11887..12333)
                     /locus_tag="IVW29_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7839149.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12330..13334)
                     /locus_tag="IVW29_001669"
     CDS             complement(12330..13334)
                     /locus_tag="IVW29_001669"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7839150.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13374..13703)
                     /locus_tag="IVW29_001670"
     CDS             complement(13374..13703)
                     /locus_tag="IVW29_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7839151.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13829..16156)
                     /locus_tag="IVW29_001671"
     CDS             complement(13829..16156)
                     /locus_tag="IVW29_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7839152.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4623 a         3241 c         2869 g         5455 t
ORIGIN      
        1 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
       61 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
      121 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
      181 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
      241 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
      301 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
      361 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
      421 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
      481 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
      541 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
      601 taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
      661 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
      721 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
      781 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
      841 tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca actccttgga ataattcagg
      901 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
      961 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     1021 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     1081 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     1141 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     1201 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     1261 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     1321 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     1381 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     1441 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     1501 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     1561 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     1621 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     1681 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     1741 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     1801 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     1861 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     1921 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     1981 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     2041 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     2101 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     2161 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     2221 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     2281 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     2341 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     2401 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     2461 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     2521 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     2581 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     2641 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     2701 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     2761 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     2821 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     2881 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     2941 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     3001 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     3061 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     3121 aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta tttcatttgt
     3181 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa actgtcattc
     3241 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     3301 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     3361 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     3421 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     3481 tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag caagtaaaac
     3541 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     3601 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     3661 gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg ctaattcttc
     3721 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     3781 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     3841 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     3901 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     3961 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     4021 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     4081 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     4141 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     4201 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     4261 ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca caccaaaagc
     4321 attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca cttttctcat
     4381 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     4441 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     4501 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     4561 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     4621 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     4681 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     4741 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     4801 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     4861 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
     4921 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
     4981 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
     5041 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
     5101 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
     5161 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
     5221 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
     5281 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
     5341 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
     5401 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
     5461 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
     5521 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
     5581 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
     5641 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
     5701 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
     5761 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     5821 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     5881 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     5941 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacactc
     6001 ttgcatttca gcaatttctt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     6061 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     6121 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     6181 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     6241 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     6301 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     6361 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     6421 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     6481 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     6541 ggtattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     6601 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     6661 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     6721 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     6781 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     6841 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     6901 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     6961 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     7021 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     7081 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     7141 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     7201 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     7261 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     7321 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     7381 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     7441 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     7501 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     7561 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     7621 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     7681 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     7741 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     7801 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     7861 tccttctatc tggcggcgac tattctttga cgtccgtcat ttcaaagtac tttactaata
     7921 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     7981 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     8041 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     8101 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     8161 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     8221 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
     8281 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
     8341 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
     8401 attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc gtttattata
     8461 taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca tacttaataa
     8521 cagaactata gcaaaaaatc ccgtgtatca acacgggatt agattaagtt tcctcaatca
     8581 ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa
     8641 aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg
     8701 tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt
     8761 cagatcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag
     8821 aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag
     8881 aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga
     8941 aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac aagagttgac
     9001 aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag aagaacggaa
     9061 aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag caatcgcaca
     9121 gcaacaagca gaacaggcga ggcaagaagc aatcgcacag caacaagcag aacaggcgcg
     9181 acagcaatca gcagctgaac aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa
     9241 taatgaacaa actgtctatg tcacaccgac cggttcaaaa taccatacac ataaatgtgg
     9301 aaatggtacg tatacccctg ccactttaag cgaagctcaa gctcgtggtt taaccccttg
     9361 ctccaaatgt tatggaaact aattcaaaag ttatactata ataataaaat tcttctgaaa
     9421 cataaaaaaa tagaagatgg cgttccagat agtaaaccaa aaataacagt ttacacgtcc
     9481 atttcgtcag cttctctctt ttttcattta gtctgttccc tctgcttttt tggacatctt
     9541 cattcattct ttctcaatca aatagtacac ctctttttct tttcgcagtt tatatcttcc
     9601 ttttttcaat ttcattgaaa actgtttcga atgtttatgt tttacatgga ttttaagcag
     9661 gagctcctca tgttgcaagc gatagttcct gccttttaaa tcagaaaatc tcactagtcc
     9721 gtattcatca gaagctttca caaatggaac tttttctcta gtaatatagt gtttgccgtt
     9781 gagcgttttt acagaaaaga ccattccttc gattggtcgt ccgtttgtgt tctttacata
     9841 aaaagataaa ggatagtttc gcttagcgat ggccaaagaa tctaaataag cgatgagtag
     9901 ccaaactaag ccgattgatc caagtagtat accgataaac caaataaaaa atttccattt
     9961 tttccattga tcaattcctt ggatcgcttt ttttgcctct tttgcttgat aaggaatacg
    10021 atgccccgtc acaagcagtc gatgtgtatt gaccatataa ggagtacagg taagcaaagt
    10081 cacgagatct ctgccttttt ggatacccaa agaatctact tcatcaggta agaccacttg
    10141 gattttttct acttgatacg ccaatgtctt tccattgatc tggatgaaaa actggtctcc
    10201 ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct gggagtccac gatgcccaga
    10261 aagaacggca tgggtgtttt tcccgccagt cggataagaa ctttcttcca ataatgacgt
    10321 tcctttttgc aaaaatattt ccgttgtctg atcgaatata ggtaagctga cattgatttt
    10381 tgggatttcg atgactccaa gcatatgttc cataaaaaag gcattactag gtaaatcttt
    10441 taatgctttt gcatcaactg cttggttgaa actgcctatc cctggtgata cgttttgctc
    10501 tgccaactgt tggtttttct ttgtcatttt ttcctgctgt ttttttattt cctctgaatt
    10561 cttattcgat gcttgctttt gataatggat gatcatttgc tgggcaagaa aatcattcag
    10621 tgaatcttct acaaaaggat aagaaaagat cccgatccca acgactaaaa tgattgccat
    10681 gaagatgtcc agtattctta ttctgctttt attcatcatg tccacctatt ttctaatatg
    10741 tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa
    10801 atagttcctc ctattttgcc gtttgttgag cgaataccaa gagaccgacc acttgtcaca
    10861 acctttctcc ccatttgaat cactatcgtt tatttatcgt atgatttttg tttccaatag
    10921 tttaagcttc tgtttcttga tggcgttttt tgaaatagat cactgctcct ccaactaaag
    10981 cagtacctac caagatgaat acgatgatcc ccataccacc agttgaaggc aagattcctt
    11041 taggttggtt atcgatcgtt gtttcatcag aagttgttgg atcatcatct actgtaaatt
    11101 cagtgtacgc agcttcattc ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa
    11161 ggcagtatgc cccttgtttc agcccttcga ttgaaaattt cccttctgaa tcagaaatca
    11221 atttcacttc ttcccagtca gaacctgtga cttcatctga ccaagcatat gggtattctc
    11281 ctgaggcaag gtttgctatt tttttcatta atttaacagt tggatctttt gctcttagaa
    11341 tagaaaattc tgcatcggct aatttttctt gcgtgttcac atcttgtttg atgaagtgga
    11401 aatcaccgta gttttggaca ttttctacag taatcgtgtt atccgtagtt gtattttccc
    11461 gattgactac accgtttgga ttcccaagat tggcttcatc tgcgccatgg atgataccgg
    11521 ttgtttgttg caattttccg ttttttacta taaaagtagt ttctttttgt ggattggtta
    11581 attgataacc atctggtgct tttgtttcaa ctaaagtata attgccgtct attaatccat
    11641 tgacactgat ggatccttca gtatcagttg tgaatgtcaa cgctgactca gcaggatcat
    11701 agagagcgac accagtgttt tcatcaaaac cggtcacgaa ttgattctct gaatttttga
    11761 tctggaattg cgcccctgct aacttatcta cccctgaaaa gttcccagct ttctggattt
    11821 tgatattgct ttctttgatg acatttttag catataggtg aatcgtgtct aaaggtttgt
    11881 cagggtctag ggtaagctta acacgatatt tggcgacgtt tcgacaccgg gctttggtgt
    11941 ttctacaaag acgtatacag catctttccc atcagattta cctggaagag tcaggttagc
    12001 taatccttca ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg gaactgctgt
    12061 gtctttactt ggatagtacg tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa
    12121 cggttcgcta ttcggaaatg tcaattccgt tccgttgttt atttgatttt ctggtgctga
    12181 agtgaatttc aatttatgca aattaaccgt aacattttct ggtgtaggtg atacggctgc
    12241 agaaacctct gcagttccta ggccgagaac gaaaggaagc agcatgatag cagttcctaa
    12301 aaacgtccat atttttgttt tgatgttttt cattttattt ccctcctatt gtttcctttt
    12361 tgttgttttt tataaaacca agctgtccct gttgaaagga ctagcaataa ccctaaaatt
    12421 gagaaacctc caagcatctc ttcgcttgtt tttggaaagc ttcctccatt tttttcattt
    12481 gatggaccat ttgtctgcat ctgaactggc gtttttccat tattcgtcgg atttgctttt
    12541 ggttcgttga tgatttccaa tggtatagga tgaagcggat cataactgcc tttttcaact
    12601 tcaaagagaa tggttgattg actcaatata tatcctttag gcggtttaat ctccactagt
    12661 tcgtaacttc caaaagacaa tccatggaca gaaaatgttc catctttact ggaagtaaaa
    12721 atcgttgctc ttgaatcatc tttggctacc caatgattta tcccgttttc ttgcgctaag
    12781 tattggtcat tttttttgac tagaaattga gcatctgcta gtttttgttt cgtttgccga
    12841 tccactttga caaattgttt gcctcctgtt tggatggcaa gatgtgtttc tactgtttga
    12901 ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt tttcactgtt ttctttcaat
    12961 gtcatttggt aacggatcgt taattttttt ccagcaaacg aactaagatc ttttccagaa
    13021 tctgataaga caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca
    13081 ccaatcaaaa aatcgatcgt ttctggttct agccataact gaggatcagc tgtatctttg
    13141 atcgtatagg ttcctatctc gttgatgttc tcaggaattg ttacggtgat gagataaggg
    13201 actttatccc catctgcaaa atctgtctgc ttattctcta ctgtttttgt taatgtgagt
    13261 gcagcaggta ttttttgttc gctctttgga tacaagtgga tggtcgtcaa tttatgatta
    13321 gatgaatctg tcacaggaag cacaacgaca aatggtgctg atttttcttt tacttaatct
    13381 gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat
    13441 gcactaaaag aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt ttctgccagt
    13501 attttttcac tatcactttt ttgtgctagt tttctttgtg cgtcttccac actacttcct
    13561 tctgaccgca gcttgtaaaa ttcatccgag acatcatata tagcgaaagt aacgccgttc
    13621 aaccctttta ctccttgaaa gttatccggg atatttattc cgtcgttttg actgatactt
    13681 ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg tgattctcta
    13741 gctgacacag tcggagaacc tataaaaaag accagtagga taaaaaagag aacagcaaat
    13801 atatccacac gtacattttt tgtttttttc atcgctgttc acctgctttt ttcttacgat
    13861 ggataaaata aaagaagcca gaaaaaataa agatcagtcc taaaaagaca aagggcagta
    13921 gacctatccc acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat
    13981 ctgttgcgat aatcgtataa ttattgttta cacttccttc gcctaaacta atcgttacta
    14041 tttgtgatcc aatatctgtc cggctgttaa ctgttagggt ataatccaat gattcgccta
    14101 agacaacacc ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg gcatcataat
    14161 taaaggcagc gtcatcgaca gtaatcactg cgttagcttt atagatggta taagttcctg
    14221 atgtgttggc tatatcagta aatgacaact tgggatttgc tgctttgact ttgtctattc
    14281 cgctattcgt tagtttaatt gtatagctgc cagcattaac catatttttt gctgattgtg
    14341 catctgctgt gtaatccata ctgctaagtg ccacttgtac tggagtaagc gaactagtga
    14401 caccctctgc tagcttgaca tggattgacg ggacacctgc ttttccatca tatgtcttac
    14461 tgccactgag ctctaccgca ccgctgattt gcttaatcgt atagacatca gatgtctgca
    14521 cgatagtaaa taaatagttt gttctggcag ctagtttagc tagtccgctt tttgtcaatt
    14581 gccaagacgt ataggttcca atttcagtag gcgcagttac ttcaactccc ttttcattca
    14641 tcgctttgaa atcagttgga ttccatgtga tttgatcaat actgagatca ttgtttaaaa
    14701 gggatgttga ttctgtttta tcattaaagg catatttaac gatcggtaca acagaaagac
    14761 catcatagta cttactgcca tttgcattga ttgatactgt tgcatttgtc ttataatata
    14821 ggatgacttc agaaatctgt tttccatttg cgtcctttac gattacattt ccatttgtat
    14881 ctaaactagc taaaacactc attgctgatt ggttctttgt ttctatcgct gaaacagctg
    14941 taaaggtata tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt
    15001 aagtaagatt cattatttta gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa
    15061 tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc gacgttactt tctagggctg
    15121 tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac tgctgttgga gtgataacca
    15181 ctttgtcacc cacatggatt ccagataatg tagtcccgta cgttccgatt tttgctgctc
    15241 caccattgac tgtataagaa aaagttcctg tccgattcgc attgacctga accgttgctg
    15301 cttgataggt tggaaaattt gaaacatttc ccttaccatc aacgatctgc ccatttttga
    15361 tcgagtaccc cgtgaatgga atatcatttg ctaactgtat accgattgaa aaagtactca
    15421 tacctcctcc ggcaggaagc cctccccatg ttgagggtgt aaaagaataa cttttatccg
    15481 cattgggtgt aactgaatcg atcgtatttc cagcggtagc cacccccata tagatacttt
    15541 ttgggaatgt gatagtagta ccatttactg tggagccaat aaacagatta tggatatctg
    15601 gacggttatc aataaaagcg attgctttgg ttacgccttg taacgcagtt ttatccgcac
    15661 tcatcgatcc gttaaccgca acaatgactc catctttggc atatatcact gtatatccat
    15721 ctggtatggt atgtccgttg aaattcgcag tggccgcaac agtactaaat gtctggcttc
    15781 tacctgtttc tgaataagct ttctcccctg ctgcttttgc tgctgtgatt tctttttctg
    15841 tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat
    15901 tggtgctttc tatggactgt atttcagaat agggtggact atttgtctcc tctgtcgttt
    15961 ggttagcaaa atttgacgtt tctgtataaa cgtttacata aatacttgat aagctcccta
    16021 agaaagtaaa aatcaagatg atcgaaatca taatgggatg taagtatttt cttttagata
    16081 cagctttgag tctagaaaca aaatgttttt gacatatacg ttggatttta ttttgagttt
    16141 tcttaatcat tttcatccct cctctcaata aaaaatcatc aaataatc
//
LOCUS       DACCVU010000057        16096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCVU010000057 DACCVU010000000
VERSION     DACCVU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.057"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            226..894
                     /locus_tag="IVW29_001672"
     CDS             226..894
                     /locus_tag="IVW29_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7839153.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="IVW29_001673"
     CDS             complement(1094..1324)
                     /locus_tag="IVW29_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839154.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="IVW29_001674"
     CDS             complement(1451..2833)
                     /locus_tag="IVW29_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7839155.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="IVW29_001675"
     CDS             complement(2989..4176)
                     /locus_tag="IVW29_001675"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7839156.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="IVW29_001676"
     CDS             complement(4183..5214)
                     /locus_tag="IVW29_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7839157.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="IVW29_001677"
     CDS             complement(5230..6684)
                     /locus_tag="IVW29_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839158.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="IVW29_001678"
     CDS             6859..7764
                     /locus_tag="IVW29_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7839159.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="IVW29_001679"
     CDS             7855..8532
                     /locus_tag="IVW29_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7839160.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8813..9709
                     /locus_tag="IVW29_001680"
     CDS             8813..9709
                     /locus_tag="IVW29_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7839161.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="IVW29_001681"
     CDS             complement(9764..11134)
                     /locus_tag="IVW29_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7839162.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEILYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="IVW29_001682"
     CDS             11372..12118
                     /locus_tag="IVW29_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7839163.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="IVW29_001683"
     CDS             12115..12954
                     /locus_tag="IVW29_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7839164.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="IVW29_001684"
     CDS             12955..13905
                     /locus_tag="IVW29_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7839165.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="IVW29_001685"
     CDS             14046..14798
                     /locus_tag="IVW29_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839166.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="IVW29_001686"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="IVW29_001686"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7839167.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3003 c         3233 g         4640 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agtatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatg
//
LOCUS       DACCVU010000058        16033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCVU010000058 DACCVU010000000
VERSION     DACCVU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.058"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            71..1495
                     /locus_tag="IVW29_001687"
     CDS             71..1495
                     /locus_tag="IVW29_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7839168.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1794..2501
                     /locus_tag="IVW29_001688"
     CDS             1794..2501
                     /locus_tag="IVW29_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7839169.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2488..2811
                     /locus_tag="IVW29_001689"
     CDS             2488..2811
                     /locus_tag="IVW29_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7839170.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2900..3937)
                     /locus_tag="IVW29_001690"
     CDS             complement(2900..3937)
                     /locus_tag="IVW29_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7839171.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4258..5241)
                     /locus_tag="IVW29_001691"
     CDS             complement(4258..5241)
                     /locus_tag="IVW29_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7839172.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5656..6426)
                     /locus_tag="IVW29_001692"
     CDS             complement(5656..6426)
                     /locus_tag="IVW29_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839173.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6419..7354)
                     /locus_tag="IVW29_001693"
     CDS             complement(6419..7354)
                     /locus_tag="IVW29_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839174.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7670..9319)
                     /locus_tag="IVW29_001694"
     CDS             complement(7670..9319)
                     /locus_tag="IVW29_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7839175.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9436..12039)
                     /locus_tag="IVW29_001695"
     CDS             complement(9436..12039)
                     /locus_tag="IVW29_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7839176.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12051..13100)
                     /locus_tag="IVW29_001696"
     CDS             complement(12051..13100)
                     /locus_tag="IVW29_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7839177.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13126..13440)
                     /locus_tag="IVW29_001697"
     CDS             complement(13126..13440)
                     /locus_tag="IVW29_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7839178.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13456..13902)
                     /locus_tag="IVW29_001698"
     CDS             complement(13456..13902)
                     /locus_tag="IVW29_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7839179.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13903..15879)
                     /locus_tag="IVW29_001699"
     CDS             complement(13903..15879)
                     /locus_tag="IVW29_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7839180.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4741 a         3254 c         2660 g         5378 t
ORIGIN      
        1 ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg
       61 taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc
      121 cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg
      181 ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac
      241 aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa
      301 ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt
      361 aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat
      421 caaagtattt gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg
      481 atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga
      541 atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg
      601 tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc
      661 agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct
      721 tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc
      781 tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc
      841 tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct
      901 tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct
      961 tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc
     1021 tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa
     1081 cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa
     1141 gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga
     1201 agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa
     1261 agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca
     1321 tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct
     1381 ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa
     1441 gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc
     1501 taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca
     1561 aatacttttt cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag
     1621 tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca
     1681 gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc
     1741 acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg
     1801 aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg
     1861 gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca
     1921 cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg
     1981 ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga
     2041 ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc
     2101 tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata
     2161 ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt
     2221 gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt
     2281 ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt
     2341 ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg
     2401 tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta acactgattg
     2461 ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt
     2521 ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa
     2581 ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct
     2641 gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc
     2701 gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg
     2761 gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa
     2821 caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa
     2881 aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact tttcctaata
     2941 tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg
     3001 aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg
     3061 ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact
     3121 cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc
     3181 ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc
     3241 gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac
     3301 ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt
     3361 caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat
     3421 tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg
     3481 cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg
     3541 tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac
     3601 gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa
     3661 aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga
     3721 ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg
     3781 tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta
     3841 caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat
     3901 aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc
     3961 aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt
     4021 ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa
     4081 agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc
     4141 tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat
     4201 tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca
     4261 ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc
     4321 atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt
     4381 gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata
     4441 atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc
     4501 cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc
     4561 atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt
     4621 gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac
     4681 ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt
     4741 ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata
     4801 gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg
     4861 tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg
     4921 gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct
     4981 catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc
     5041 ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc
     5101 tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg
     5161 aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc
     5221 tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc
     5281 aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag
     5341 cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc
     5401 ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga
     5461 aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt
     5521 cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg
     5581 acaaaatatg aggaactatt ttcccgtttg tttctctaat aatcaggtca aaacgctgat
     5641 ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac
     5701 ggcgtacaaa acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc
     5761 gattgttcca aggaatccgt aacggaaacc gaagatcata taaacgatcg gattgacttt
     5821 cgaaacagct tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata
     5881 agttaacggc tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa
     5941 aatcccgttg agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat
     6001 gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag
     6061 agagccgacg agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg
     6121 cgtaggaaca ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa
     6181 ggacgaagaa acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac
     6241 gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc
     6301 gaaaataaca aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat
     6361 acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt
     6421 aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta
     6481 aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga
     6541 agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa
     6601 atgtacaagc gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa
     6661 atgtttcaaa ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac
     6721 ctgattggat gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg
     6781 tcaaaatgat cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc
     6841 tcaattcaat atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca
     6901 tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga
     6961 catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt
     7021 ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa
     7081 agggattgaa attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat
     7141 ccgtatccag atcataaccg aagacattca cttttccaga tgttttattg actaaagaag
     7201 taatgattcc gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat
     7261 ctccttcttt cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct
     7321 ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg
     7381 attctttatt gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat
     7441 caggttgctg ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca
     7501 accttttcag aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg
     7561 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aagcaaaagc caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata
     7681 catgccactg ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag
     7741 tctctgggta atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt
     7801 cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga
     7861 cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg
     7921 ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga
     7981 tcgtatttgt cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca
     8041 cttggtccaa caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa
     8101 ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt
     8161 ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat
     8221 acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg
     8281 gattcactac cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct
     8341 cagtaaactc tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg
     8401 ctacttctaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta
     8461 atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt
     8521 caaaaatcaa ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga
     8581 tgtctcttaa tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg
     8641 ctagctgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt
     8701 ctagtgtttg tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca
     8761 aatagattcc tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc
     8821 caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt
     8881 gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc
     8941 tcagttgagg attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa
     9001 aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt
     9061 tgattccttc gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc
     9121 tatcccatga acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat
     9181 caaatctcgg ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca
     9241 aacgtgaaaa aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga
     9301 taaattctct ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac
     9361 tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc
     9421 ttgtatatta catatttatc tctcatttat caaaattgac aaataatcat agggaggaat
     9481 tgttgttaat tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc
     9541 ctcaggtact attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc
     9601 atgaaaataa gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga
     9661 aaatttacga ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg
     9721 aataaataca ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc
     9781 ctgttttgcc actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac
     9841 tttatactca cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt
     9901 cgtatctcct gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt
     9961 tgttgaaaat aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc
    10021 tgcataaaac gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc
    10081 taaattaaca ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt
    10141 attttcaata aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt
    10201 aattgctaag cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac
    10261 gatttgactt tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt
    10321 ttcagataaa cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt
    10381 ttcttgtaaa gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc
    10441 tccttctaat ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac
    10501 gaaatcaaga tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag
    10561 tttatattta ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc
    10621 attatttttc tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt
    10681 tatgtcaagt tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg
    10741 ataagcattt ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc
    10801 tttgaagtgt attttctttg aatcagctac tacatgcacg attttcgatc cactataatt
    10861 ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat
    10921 ccgttttaca ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg
    10981 gaaaatatct tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt
    11041 ttgcaaaatc tttttccata accgacgaag taggccttta ctgatatgga taccatttct
    11101 ttctgcaatc agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt
    11161 ttctattttc atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg
    11221 ctgacgaagt tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata
    11281 agaaggataa tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac
    11341 ttgatcaaaa tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt
    11401 gttttttcct tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc
    11461 aggaatatct ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt
    11521 catggcataa acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat
    11581 cgtttcaaag tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag
    11641 tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc
    11701 tccatattta aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc
    11761 atccactaat aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt
    11821 catcagtcgt tggataacct taagcttttg cggattgatc tccaaatact catcgacgat
    11881 tgaagtttgt ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac
    11941 ttcagtaaag acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct
    12001 gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct
    12061 tcttctactg aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc
    12121 attaacgctg taaccactcc cccaataaca attgatacta aatataaagg tatattcgta
    12181 acaaaaggaa ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca
    12241 tttagaccac atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct
    12301 ttcacggcaa acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc
    12361 tttgcattat ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac
    12421 attccaaatg gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg
    12481 cttgaaaaag aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca
    12541 aacgccatca ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct
    12601 tctagccaat tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat
    12661 aatatgattc caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta
    12721 ataacttgca tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat
    12781 ccagcaacca ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata
    12841 aaagctggtg ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc
    12901 ggtacgataa actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc
    12961 gtctggtccc aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct
    13021 gctacgacaa gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt
    13081 ccttgtttct tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa
    13141 tgcaattgct tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt
    13201 taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat
    13261 gacaacatct gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc
    13321 ctgtgtttct actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt
    13381 tgccatataa gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat
    13441 atgaaattcc tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc
    13501 ttttattctt ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg
    13561 taaatgatta gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg
    13621 ttgatcatct aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga
    13681 ttcaattact gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt
    13741 catatggttt tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc
    13801 attagtctct aacaatttcg ataatttttc gactacactt tttttatcag atgcatttaa
    13861 atctatcact atattattta tattgatttt ttcactgatc atttataagc cttctttcaa
    13921 tatataaact aatttttcaa acgacgaact cgtttttaaa tttgctaata actcatgatt
    13981 cgaagcgatt tcattgaatg tttcataaat tttattcaat tcaggtttta ctttttcatt
    14041 aagtgcaaaa aagaaaacag aatgaacaag tgtcccatcc caatcaattc cttctggatt
    14101 aacgtagaca cagattactg gcttttttat aaaatctact tcgatatgcg gcatagcaaa
    14161 atcattcatc gctgttgaag atagtttttc tcttttgatt gctccctcta gtatcccttc
    14221 tttcgcgtaa ttttctttta ccaatttttt ggtaattata tttaaaacgt tcacttgatt
    14281 atcctttatt tggtgggtaa aaaagcaaaa tcgtttatct aataaattta gaaaagcatc
    14341 ttctgtttgt tcatttaact gtagatactg ttttactttt ctgacatctg cccctaacat
    14401 caaaggacta acaacaataa caggaaaatt tgtattttca atcggaatcg tgcttataac
    14461 aagttcatca gtgacttttc tatcttgtag ttcattgatg cttagtacat cttctatctc
    14521 aaccatatct gcaaaaccgt tatttattct ttgctctaat aattttgatg ttccatatcc
    14581 gctactacaa actaatatca ctttcgtttt gtcaggtttg taacgatcca aaagtgattg
    14641 aatatggagt gttacgtaag cgatttcatc ttcattaaaa tgtatacaga atctctcttc
    14701 gatttcctct aataaatcta cacttatcat aaatgacctt ttaaaagaat atttgatttt
    14761 gtccgtataa gggttataga tatttactcc caatttgagt cttttaactg ctgcattgag
    14821 atgtaatact agattattga tcaattcttc atcaggataa aattcattta atttaattct
    14881 tactaactct tgtagctcaa cacccatatc taaataggaa agcttcgttt gatctaagtc
    14941 gtgattcata gcattttttt caatcgaagt aatgtgaatg tcaacataag cttgttcatc
    15001 tattggcaca ataattgaaa atgctttttc aatcattttg attaaaataa gtgtattctt
    15061 tgagatttct tcttcagaat tattactacg tgataagaaa ttgcttgttt caatcctttc
    15121 tatcgcaata gcaaggtgga tagctaaagt ttgaaattca taatctgtaa atttcaattg
    15181 tgtcttattc atgtatcgat taagcaattc ggctactttt ttcaatatgt ccttatttaa
    15241 gaaattattc gcttcaccaa tgaaattgat attcaagtct aagagatctg ttttactgtt
    15301 agatgctgta aagccacccc aataatagtt cacaacttct gacatcaacc ttcttttact
    15361 tttttcatca gctttgatgc gcacgccatt tcgatctgaa actaaggaca tcccatgctt
    15421 gtttagtctt tttctaacat ctttcattgt gctttctacc gtcgacctgc tcacgtataa
    15481 ttcgtcagca agattttgaa tctttagaga atcattagta ttcaataatt tagcaaacac
    15541 ataagttaga cggtcctccg gagagtctgc gatattgatt ttatgactgg ataataaatt
    15601 tcgaagtgtt tctttttttc caataagcga aacgcctttt ttaggttgaa ccaacatttg
    15661 tacatctggc attttttgga ttatttcttt caaaattaaa atatctttgc taattgtttt
    15721 cgtagataca tataattgtt ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa
    15781 aaagtatagt attttcattt gccgttcgct aaattcgatg cccattatct tcacctcctg
    15841 aatacagctt aagtgtacaa ttattatctc tattttcata cagaagaaag ttcttgatta
    15901 atgtagaagt ttctaatatt cttgattatt tcaaatcaat aaaaaagcct gcaatattta
    15961 tatcacaggc ttttttctct attaaatata tataagataa tacatttttc tactagttct
    16021 cactaatctt gta
//
LOCUS       DACCVU010000059        15961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCVU010000059 DACCVU010000000
VERSION     DACCVU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.059"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            291..1430
                     /locus_tag="IVW29_001700"
     CDS             291..1430
                     /locus_tag="IVW29_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7839181.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IVW29_001701"
     CDS             complement(1497..2072)
                     /locus_tag="IVW29_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7839182.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IVW29_001702"
     CDS             complement(2077..3051)
                     /locus_tag="IVW29_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7839183.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IVW29_001703"
     CDS             3334..4302
                     /locus_tag="IVW29_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7839184.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IVW29_001704"
     CDS             4415..4693
                     /locus_tag="IVW29_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7839185.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IVW29_001705"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IVW29_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7839186.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IVW29_001706"
     CDS             5159..5905
                     /locus_tag="IVW29_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7839187.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IVW29_001707"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IVW29_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7839188.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IVW29_001708"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IVW29_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7839189.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IVW29_001709"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IVW29_001709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7839190.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IVW29_001710"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IVW29_001710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7839191.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IVW29_001711"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IVW29_001711"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7839192.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IVW29_001712"
     CDS             10642..11184
                     /locus_tag="IVW29_001712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7839193.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IVW29_001713"
     CDS             11207..12763
                     /locus_tag="IVW29_001713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7839194.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IVW29_001714"
     CDS             12777..13679
                     /locus_tag="IVW29_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7839195.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IVW29_001715"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IVW29_001715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7839196.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IVW29_001716"
     CDS             15122..15544
                     /locus_tag="IVW29_001716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7839197.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5269 a         2878 c         3446 g         4368 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 g
//
LOCUS       DACCVU010000060        15930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCVU010000060 DACCVU010000000
VERSION     DACCVU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.060"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(183..1082)
                     /locus_tag="IVW29_001717"
     CDS             complement(183..1082)
                     /locus_tag="IVW29_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7839198.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1092..1448)
                     /locus_tag="IVW29_001718"
     CDS             complement(1092..1448)
                     /locus_tag="IVW29_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7839199.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1803..2099
                     /locus_tag="IVW29_001719"
     CDS             1803..2099
                     /locus_tag="IVW29_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7839200.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2743..3696)
                     /locus_tag="IVW29_001720"
     CDS             complement(2743..3696)
                     /locus_tag="IVW29_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7839201.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3719..4474)
                     /locus_tag="IVW29_001721"
     CDS             complement(3719..4474)
                     /locus_tag="IVW29_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7839202.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4471..5430)
                     /locus_tag="IVW29_001722"
     CDS             complement(4471..5430)
                     /locus_tag="IVW29_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7839203.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5423..6373)
                     /locus_tag="IVW29_001723"
     CDS             complement(5423..6373)
                     /locus_tag="IVW29_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7839204.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6523..6975
                     /locus_tag="IVW29_001724"
     CDS             6523..6975
                     /locus_tag="IVW29_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7839205.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7064..9331)
                     /gene="gshAB"
                     /locus_tag="IVW29_001725"
     CDS             complement(7064..9331)
                     /gene="gshAB"
                     /locus_tag="IVW29_001725"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7839206.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9518..10516)
                     /gene="ftsY"
                     /locus_tag="IVW29_001726"
     CDS             complement(9518..10516)
                     /gene="ftsY"
                     /locus_tag="IVW29_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7839207.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10527..11336)
                     /locus_tag="IVW29_001727"
     CDS             complement(10527..11336)
                     /locus_tag="IVW29_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7839208.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11345..14926)
                     /gene="smc"
                     /locus_tag="IVW29_001728"
     CDS             complement(11345..14926)
                     /gene="smc"
                     /locus_tag="IVW29_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7839209.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14946..15632)
                     /locus_tag="IVW29_001729"
     CDS             complement(14946..15632)
                     /locus_tag="IVW29_001729"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7839210.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
BASE COUNT         4490 a         3373 c         2770 g         5297 t
ORIGIN      
        1 gttcaaaaaa agaatacacg cttgcatcta gtactctgcg agttacaaat catccgatca
       61 aataaacatt cgctgattat tttccgcaaa aaaagcagca tatccacgat aaatggatct
      121 actgctctta cttatcctta tgttttacaa ggtattttct tttacacatt actaatgctg
      181 tctcaatacg gaacgattgt catcgcttgt tcaaattcct cttgcaaatg ttgtgtttga
      241 taggcctgaa aagctgggtg atgggcacgc aagaggacac gtaatttcat aggatcatag
      301 ctgtctagca actgttcttt caagacaaaa tctcgggtca gctgccattc ataataataa
      361 ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa gcgacgatgc
      421 gtatggccaa aaactacttg tttgatattt gaataacggt caagtaattc tccgaatgat
      481 tctgatccta aaaatgcatt taatttgttc caacgctcat aaggagcaga ttgatacatg
      541 ataaatgccc gtttaggaac gaaatgagtg gataaaatca cctcaagttt ttttgcttcc
      601 agctggtcta gcaaattttt tgcagctgca agcatttctg cattgacctc gagatctgtt
      661 ttcccacgct ggatgaaccg atcataccaa tacaaacgct tcattcgtac gattgcctcg
      721 taatctttca attcggaaaa ctgatagtcg taccagccat tcatccctaa aagaacggtc
      781 tgctcattta aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa atggtccatc
      841 atctgttcat tgggcacatc tgccaactcg tgattgccga aatgaaatgt cgtagataat
      901 cctttattga cgaaaaattc gacaacagct aggcaacggt ctactttatt agccgtatct
      961 ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag ctgcatcagc
     1021 tctgattcgc cgaatttatt gatgtccaca tgtaggtcgc tgatgatcgc caattttccc
     1081 attttatccc tctagtcttt tcgaacaact tgcaagttgc cttttctcaa gccgcttggt
     1141 ccttttaatt gtaaactatt ataagttggt tctaaagcaa gtgtggatgg atcatccatg
     1201 taaagtattg cactgtcttt gattttgatt ggtccaactg gtgtatcagc cactacagaa
     1261 taaagactta gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa tgccgtatcc
     1321 aattggcaac tgacttggct ttctgcaaat ggaccgtcac cgttttcgat atctaataaa
     1381 agttgatcgt ctgcttctaa tttattcttc aatacttctt gtgcttcttt tgtcacttct
     1441 aatttcatct gtatcacctc atgtcataga ataatcactt tattcatcag atgcaactaa
     1501 tttgtctggt tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt tattatgatt
     1561 tctcttatca caaaagagac aactttccct tttcttcgtt cagtttcttg aacggaaaaa
     1621 gacccaaaaa gcaacctgtt atttgtttaa ccttattcct ttcttaaata acaaaaaata
     1681 ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga
     1741 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
     1801 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
     1861 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
     1921 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
     1981 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
     2041 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
     2101 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
     2161 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
     2221 gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt
     2281 ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat
     2341 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
     2401 tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg
     2461 ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac
     2521 tattttttgc cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt
     2581 tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg
     2641 gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat
     2701 tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt
     2761 cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc
     2821 tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt
     2881 ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa
     2941 taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga
     3001 tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct
     3061 agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt
     3121 gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg
     3181 agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa
     3241 agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag
     3301 atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt
     3361 aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga
     3421 aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc
     3481 atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac
     3541 ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct
     3601 tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga
     3661 tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt
     3721 agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata
     3781 attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc
     3841 catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa
     3901 caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa
     3961 ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt
     4021 gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg
     4081 attgatccgc tatttcttct aacccaatgt gttccagtga ttcctgaacg atttcctcgt
     4141 cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca
     4201 cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt
     4261 cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg
     4321 gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa
     4381 aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat
     4441 ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa
     4501 ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca
     4561 gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa
     4621 gcttccgata aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc
     4681 gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag
     4741 actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc
     4801 ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca
     4861 gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct
     4921 tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa
     4981 ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag
     5041 attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag
     5101 cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat
     5161 aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg
     5221 agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag
     5281 cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt
     5341 tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc
     5401 aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga
     5461 agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca
     5521 acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc
     5581 caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc
     5641 cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat gatactaagc
     5701 caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc
     5761 ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa
     5821 cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg
     5881 aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga
     5941 tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc
     6001 gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc
     6061 ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac
     6121 tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga
     6181 tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac
     6241 gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta
     6301 cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc
     6361 gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt
     6421 gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct
     6481 ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta
     6541 aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga agaacagcat
     6601 aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc
     6661 caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata
     6721 ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa
     6781 aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga
     6841 ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac
     6901 ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg
     6961 gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat
     7021 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     7081 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat
     7141 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     7201 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     7261 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     7321 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     7381 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     7441 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     7501 cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca
     7561 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     7621 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     7681 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     7741 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     7801 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     7861 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     7921 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     7981 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     8041 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     8101 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     8161 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     8221 aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt
     8281 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     8341 taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca
     8401 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     8461 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     8521 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     8581 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     8641 ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat
     8701 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     8761 tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt
     8821 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     8881 attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat tcttttgcta
     8941 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt
     9001 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     9061 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     9121 taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     9181 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     9241 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     9301 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     9361 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     9421 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     9481 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     9541 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     9601 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     9661 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     9721 attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt
     9781 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     9841 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     9901 ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc
     9961 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
    10021 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
    10081 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
    10141 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
    10201 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
    10261 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
    10321 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
    10381 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
    10441 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
    10501 tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat cactttggct
    10561 acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca
    10621 gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca
    10681 tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata
    10741 ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg
    10801 atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct
    10861 tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc
    10921 gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca
    10981 ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga
    11041 gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca
    11101 ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct
    11161 accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga
    11221 acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc
    11281 agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc
    11341 gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag
    11401 aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga
    11461 ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca
    11521 agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga
    11581 tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg
    11641 ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt
    11701 caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac
    11761 cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt
    11821 ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt
    11881 tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt
    11941 cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg
    12001 atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt
    12061 tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt
    12121 cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac
    12181 gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct
    12241 gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa
    12301 tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg
    12361 ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac
    12421 cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt
    12481 cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac
    12541 ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt
    12601 ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct
    12661 caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta
    12721 gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt
    12781 cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg
    12841 cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt
    12901 gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg
    12961 cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga
    13021 ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca
    13081 cagtttggat ttgatcggga taactgactt gttcactggc aatccctata aacccttcta
    13141 cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg
    13201 gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat
    13261 ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta
    13321 acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct
    13381 gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga
    13441 tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca
    13501 ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt
    13561 ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt
    13621 cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg
    13681 tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc
    13741 ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt
    13801 ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag
    13861 ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt
    13921 caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt
    13981 tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt
    14041 tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat
    14101 tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc
    14161 ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg
    14221 tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta
    14281 aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt
    14341 ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt
    14401 tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt
    14461 cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt
    14521 cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt
    14581 tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat
    14641 tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat
    14701 tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca
    14761 gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc
    14821 cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt
    14881 ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact
    14941 tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat
    15001 ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt
    15061 tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt
    15121 tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg
    15181 actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga
    15241 tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct
    15301 cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca
    15361 aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct
    15421 ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct
    15481 cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag
    15541 gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt
    15601 tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt
    15661 tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta
    15721 agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg
    15781 agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa
    15841 taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga
    15901 cctgaatatt agagaaataa acagaaaaat
//
LOCUS       DACCVU010000061        15566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCVU010000061 DACCVU010000000
VERSION     DACCVU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.061"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(66..785)
                     /locus_tag="IVW29_001730"
     CDS             complement(66..785)
                     /locus_tag="IVW29_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7839211.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(916..2067)
                     /locus_tag="IVW29_001731"
     CDS             complement(916..2067)
                     /locus_tag="IVW29_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7839212.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2284..3024)
                     /locus_tag="IVW29_001732"
     CDS             complement(2284..3024)
                     /locus_tag="IVW29_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7839213.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3028..3351)
                     /gene="rsfS"
                     /locus_tag="IVW29_001733"
     CDS             complement(3028..3351)
                     /gene="rsfS"
                     /locus_tag="IVW29_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7839214.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3408..4001)
                     /locus_tag="IVW29_001734"
     CDS             complement(3408..4001)
                     /locus_tag="IVW29_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7839215.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3985..4632)
                     /locus_tag="IVW29_001735"
     CDS             complement(3985..4632)
                     /locus_tag="IVW29_001735"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7839216.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4645..4956)
                     /gene="yhbY"
                     /locus_tag="IVW29_001736"
     CDS             complement(4645..4956)
                     /gene="yhbY"
                     /locus_tag="IVW29_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7839217.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4972..6081)
                     /gene="yqeH"
                     /locus_tag="IVW29_001737"
     CDS             complement(4972..6081)
                     /gene="yqeH"
                     /locus_tag="IVW29_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7839218.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6078..6605)
                     /locus_tag="IVW29_001738"
     CDS             complement(6078..6605)
                     /locus_tag="IVW29_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7839219.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6785..7570)
                     /locus_tag="IVW29_001739"
     CDS             complement(6785..7570)
                     /locus_tag="IVW29_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7839220.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7563..8432)
                     /locus_tag="IVW29_001740"
     CDS             complement(7563..8432)
                     /locus_tag="IVW29_001740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7839221.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8433..9812)
                     /locus_tag="IVW29_001741"
     CDS             complement(8433..9812)
                     /locus_tag="IVW29_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7839222.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9820..10239)
                     /gene="fabZ"
                     /locus_tag="IVW29_001742"
     CDS             complement(9820..10239)
                     /gene="fabZ"
                     /locus_tag="IVW29_001742"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7839223.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10264..10740)
                     /gene="accB"
                     /locus_tag="IVW29_001743"
     CDS             complement(10264..10740)
                     /gene="accB"
                     /locus_tag="IVW29_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7839224.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10743..11978)
                     /gene="fabF"
                     /locus_tag="IVW29_001744"
     CDS             complement(10743..11978)
                     /gene="fabF"
                     /locus_tag="IVW29_001744"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7839225.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(11997..12734)
                     /gene="fabG"
                     /locus_tag="IVW29_001745"
     CDS             complement(11997..12734)
                     /gene="fabG"
                     /locus_tag="IVW29_001745"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7839226.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12746..13663)
                     /gene="fabD"
                     /locus_tag="IVW29_001746"
     CDS             complement(12746..13663)
                     /gene="fabD"
                     /locus_tag="IVW29_001746"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7839227.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13770..13994)
                     /locus_tag="IVW29_001747"
     CDS             complement(13770..13994)
                     /locus_tag="IVW29_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7839228.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14047..15009)
                     /locus_tag="IVW29_001748"
     CDS             complement(14047..15009)
                     /locus_tag="IVW29_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7839229.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15019..15459)
                     /locus_tag="IVW29_001749"
     CDS             complement(15019..15459)
                     /locus_tag="IVW29_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7839230.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4074 a         3552 c         2675 g         5265 t
ORIGIN      
        1 aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg
       61 gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc
      121 agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt
      181 tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct
      241 tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc
      301 attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct
      361 tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca
      421 cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca
      481 ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc
      541 aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata
      601 tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca
      661 tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa
      721 atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt
      781 cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc
      841 attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca
      901 tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt
      961 gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt
     1021 ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc
     1081 atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt
     1141 tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt
     1201 tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat
     1261 gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga
     1321 taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga
     1381 aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc
     1441 atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc
     1501 tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt
     1561 tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata
     1621 cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc
     1681 ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta
     1741 atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa
     1801 gatttttact gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag
     1861 ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat
     1921 gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc
     1981 tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta
     2041 ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag
     2101 aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat
     2161 tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg
     2221 ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa
     2281 ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc
     2341 tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata
     2401 actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga
     2461 cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc
     2521 tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg
     2581 aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc
     2641 taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca
     2701 cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat
     2761 attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat
     2821 caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac
     2881 cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg
     2941 acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat
     3001 gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg
     3061 agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg
     3121 tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg
     3181 ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat
     3241 ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg
     3301 aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt
     3361 taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct
     3421 acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa
     3481 tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg
     3541 gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa
     3601 atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga
     3661 agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca
     3721 tgccagattg cattattata attcaataac tccggatcaa aaccgtctct tacgattacc
     3781 attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta
     3841 gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca
     3901 agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct
     3961 ctagaataag cagtatattc attactatag tccatattca tacaaccctt cattttgaat
     4021 ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg
     4081 acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg
     4141 agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc
     4201 atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata
     4261 atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc
     4321 accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc
     4381 caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg
     4441 ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat
     4501 cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt
     4561 cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc
     4621 ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata
     4681 cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc
     4741 gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg
     4801 aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat
     4861 tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct
     4921 acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa
     4981 tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc
     5041 tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa
     5101 ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa
     5161 tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt
     5221 tacatcattt gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct
     5281 tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat
     5341 ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg
     5401 gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat
     5461 tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt
     5521 atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata
     5581 gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga
     5641 tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc
     5701 cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt
     5761 cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc
     5821 attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt
     5881 cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg
     5941 taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt
     6001 ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat
     6061 acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct
     6121 aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct
     6181 gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata
     6241 tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc
     6301 tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca
     6361 aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg
     6421 attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta
     6481 ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt
     6541 aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa
     6601 aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg
     6661 tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag
     6721 catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa
     6781 acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc
     6841 gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc
     6901 gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc
     6961 gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt
     7021 tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca
     7081 tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga
     7141 tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct
     7201 ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg
     7261 taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc
     7321 cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt
     7381 gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt
     7441 cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa
     7501 aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg
     7561 tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc
     7621 tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca
     7681 gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca
     7741 gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg
     7801 gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg
     7861 ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct
     7921 tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct
     7981 cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa
     8041 ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg
     8101 actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga
     8161 aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca
     8221 ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat
     8281 actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc
     8341 cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc
     8401 cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt
     8461 gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact
     8521 gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata
     8581 aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg
     8641 attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca
     8701 ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga
     8761 gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct
     8821 tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt
     8881 ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga
     8941 gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg
     9001 gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt
     9061 tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt
     9121 aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga
     9181 acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat
     9241 gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga
     9301 actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca
     9361 attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga
     9421 atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca
     9481 tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca catggaggca
     9541 aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg
     9601 attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca
     9661 atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat
     9721 atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca
     9781 ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa
     9841 ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga
     9901 agctttcact ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt
     9961 acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag
    10021 agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat
    10081 aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt
    10141 agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata
    10201 acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt
    10261 tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc
    10321 tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat
    10381 cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc
    10441 tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat
    10501 tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc
    10561 aggtatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat
    10621 gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa
    10681 ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa
    10741 aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa
    10801 ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca
    10861 atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc
    10921 agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat
    10981 ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt
    11041 gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt
    11101 tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca
    11161 tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct
    11221 ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct
    11281 gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta
    11341 ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa
    11401 atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg
    11461 aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata
    11521 ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt
    11581 acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg
    11641 attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt
    11701 ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc
    11761 gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct
    11821 ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg
    11881 atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt
    11941 gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc cttttcttat
    12001 ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt
    12061 gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt
    12121 tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg
    12181 aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat
    12241 tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca
    12301 atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt
    12361 ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg
    12421 aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact
    12481 aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca
    12541 taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct
    12601 atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca
    12661 aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt
    12721 ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct
    12781 tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata
    12841 cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac
    12901 tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt
    12961 tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa
    13021 tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag
    13081 gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag
    13141 cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg
    13201 caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg
    13261 gcgcattcat cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag
    13321 cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta
    13381 atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct
    13441 tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct
    13501 gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac
    13561 ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt
    13621 gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat
    13681 ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa
    13741 tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact
    13801 aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca
    13861 atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca
    13921 gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt
    13981 ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt tttataattt
    14041 aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa
    14101 tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa
    14161 gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc
    14221 ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa
    14281 aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg
    14341 tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc
    14401 ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt
    14461 accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat
    14521 cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt
    14581 agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc
    14641 agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc
    14701 atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg
    14761 cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat
    14821 caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg
    14881 ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag
    14941 atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa
    15001 ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc
    15061 atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc
    15121 acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt
    15181 tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt
    15241 gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt
    15301 tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc
    15361 tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact
    15421 aacaagataa tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag
    15481 tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct
    15541 taaaagatca tttttttact atatga
//
LOCUS       DACCVU010000062        15363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCVU010000062 DACCVU010000000
VERSION     DACCVU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.062"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            82..2142
                     /locus_tag="IVW29_001750"
     CDS             82..2142
                     /locus_tag="IVW29_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839231.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2225..2794
                     /locus_tag="IVW29_001751"
     CDS             2225..2794
                     /locus_tag="IVW29_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839232.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2969..3775)
                     /locus_tag="IVW29_001752"
     CDS             complement(2969..3775)
                     /locus_tag="IVW29_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839233.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3913..5427
                     /locus_tag="IVW29_001753"
     CDS             3913..5427
                     /locus_tag="IVW29_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7839234.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5830..6813)
                     /locus_tag="IVW29_001754"
     CDS             complement(5830..6813)
                     /locus_tag="IVW29_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7839235.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6818..7465)
                     /locus_tag="IVW29_001755"
     CDS             complement(6818..7465)
                     /locus_tag="IVW29_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7839236.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7550..8182)
                     /locus_tag="IVW29_001756"
     CDS             complement(7550..8182)
                     /locus_tag="IVW29_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7839237.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="IVW29_001757"
     CDS             complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="IVW29_001757"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7839238.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8746..9483)
                     /locus_tag="IVW29_001758"
     CDS             complement(8746..9483)
                     /locus_tag="IVW29_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7839239.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9480..10469)
                     /locus_tag="IVW29_001759"
     CDS             complement(9480..10469)
                     /locus_tag="IVW29_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7839240.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10727..11269
                     /locus_tag="IVW29_001760"
     CDS             10727..11269
                     /locus_tag="IVW29_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839241.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11286..12371
                     /locus_tag="IVW29_001761"
     CDS             11286..12371
                     /locus_tag="IVW29_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839242.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12383..13189
                     /locus_tag="IVW29_001762"
     CDS             12383..13189
                     /locus_tag="IVW29_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839243.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13190..14017
                     /locus_tag="IVW29_001763"
     CDS             13190..14017
                     /locus_tag="IVW29_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839244.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14014..15087
                     /locus_tag="IVW29_001764"
     CDS             14014..15087
                     /locus_tag="IVW29_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7839245.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4933 a         2775 c         3136 g         4519 t
ORIGIN      
        1 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
       61 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      121 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      181 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      241 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      301 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      361 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      421 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      481 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      541 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      601 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      661 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      721 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      781 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      841 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      901 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
      961 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1021 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1081 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1141 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1201 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1261 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1321 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1381 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1441 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1501 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1561 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1621 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1681 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1741 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1801 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1861 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1921 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     1981 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2041 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2101 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2161 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2221 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2281 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2341 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2401 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2461 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2521 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa
     2581 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2641 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2701 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2761 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2821 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2881 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     2941 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3001 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3061 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3121 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3181 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3241 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3301 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3361 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3421 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3481 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3541 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3601 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3661 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3721 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3781 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3841 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3901 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     3961 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4021 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4081 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4141 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4201 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4261 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4321 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4381 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4441 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4501 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4561 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4621 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4681 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4741 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4801 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4861 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4921 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     4981 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5041 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5101 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5161 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5221 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5281 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5341 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5401 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5461 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5521 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
     5581 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa
     5641 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     5701 gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     5761 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc
     5821 agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat
     5881 atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat
     5941 cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt
     6001 atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa
     6061 tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg
     6121 tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa
     6181 aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt
     6241 tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga
     6301 tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt
     6361 tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag
     6421 ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata
     6481 gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata
     6541 accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact
     6601 tttattctca actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa
     6661 agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat
     6721 cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac
     6781 agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt
     6841 ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat
     6901 ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg
     6961 atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg
     7021 tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat
     7081 gtttagtaat ataataatct gtcatggact caggccattt tgctggttca tctgcataaa
     7141 cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga
     7201 ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca
     7261 atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag
     7321 agacatacga aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg
     7381 caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt
     7441 ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc
     7501 aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa
     7561 gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg
     7621 cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag
     7681 catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat
     7741 tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg
     7801 aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc
     7861 caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc
     7921 cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac
     7981 ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa
     8041 cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga
     8101 taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg
     8161 taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag
     8221 tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc
     8281 cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt
     8341 ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca
     8401 attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc
     8461 cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt
     8521 gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat
     8581 ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt
     8641 tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc
     8701 ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc
     8761 cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac
     8821 tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc
     8881 atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag
     8941 agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca
     9001 ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt
     9061 gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc
     9121 cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact
     9181 aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt
     9241 tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt
     9301 tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt
     9361 atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt
     9421 tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt
     9481 catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta
     9541 gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc
     9601 tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa
     9661 tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg
     9721 attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga
     9781 ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa
     9841 gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt
     9901 tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt
     9961 ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact
    10021 tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac
    10081 cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac
    10141 cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca
    10201 aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca
    10261 gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg
    10321 ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca
    10381 ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt
    10441 attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt
    10501 atcataccat aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag
    10561 gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca
    10621 aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta
    10681 gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga
    10741 gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac
    10801 agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga
    10861 aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga
    10921 agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga
    10981 aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt
    11041 cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac
    11101 gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc
    11161 aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct
    11221 caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta
    11281 aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa
    11341 cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc
    11401 cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag
    11461 aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag
    11521 taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg
    11581 cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg
    11641 ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg
    11701 gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat
    11761 tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc
    11821 gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag
    11881 cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta
    11941 cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga
    12001 gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat
    12061 ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag
    12121 aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc
    12181 tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg
    12241 tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag
    12301 atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct
    12361 acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat
    12421 tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt
    12481 tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc
    12541 gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat
    12601 catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat
    12661 gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat
    12721 tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca
    12781 gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat
    12841 gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag
    12901 agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa
    12961 tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac
    13021 tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt
    13081 ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt
    13141 catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt
    13201 taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta
    13261 tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg
    13321 ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt
    13381 catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg
    13441 aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt
    13501 attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg
    13561 attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt
    13621 cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag
    13681 agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc
    13741 aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac
    13801 atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag
    13861 acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat
    13921 attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa
    13981 cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta
    14041 ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt
    14101 ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt
    14161 ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct
    14221 aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca
    14281 agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg
    14341 aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt tgatccagat
    14401 aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa cgataaattc
    14461 tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt
    14521 ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat
    14581 tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta
    14641 acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa
    14701 gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat
    14761 ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca
    14821 aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa
    14881 aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg
    14941 ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc
    15001 catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc
    15061 ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa
    15121 cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa
    15181 gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag
    15241 agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt
    15301 gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt
    15361 aat
//
LOCUS       DACCVU010000063        15346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCVU010000063 DACCVU010000000
VERSION     DACCVU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.063"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(41..406)
                     /locus_tag="IVW29_001765"
     CDS             complement(41..406)
                     /locus_tag="IVW29_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839246.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            614..1096
                     /locus_tag="IVW29_001766"
     CDS             614..1096
                     /locus_tag="IVW29_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839247.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1293..2183
                     /gene="pstS"
                     /locus_tag="IVW29_001767"
     CDS             1293..2183
                     /gene="pstS"
                     /locus_tag="IVW29_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7839248.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2508..4979
                     /locus_tag="IVW29_001768"
     CDS             2508..4979
                     /locus_tag="IVW29_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7839249.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5082..6818)
                     /locus_tag="IVW29_001769"
     CDS             complement(5082..6818)
                     /locus_tag="IVW29_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7839250.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6815..7519)
                     /locus_tag="IVW29_001770"
     CDS             complement(6815..7519)
                     /locus_tag="IVW29_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839251.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7650..8153
                     /locus_tag="IVW29_001771"
     CDS             7650..8153
                     /locus_tag="IVW29_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7839252.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8113..8451
                     /locus_tag="IVW29_001772"
     CDS             8113..8451
                     /locus_tag="IVW29_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7839253.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8472..9890
                     /gene="ffh"
                     /locus_tag="IVW29_001773"
     CDS             8472..9890
                     /gene="ffh"
                     /locus_tag="IVW29_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7839254.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10002..10580
                     /locus_tag="IVW29_001774"
     CDS             10002..10580
                     /locus_tag="IVW29_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7839255.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10584..11105
                     /locus_tag="IVW29_001775"
     CDS             10584..11105
                     /locus_tag="IVW29_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7839256.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11184..11738)
                     /locus_tag="IVW29_001776"
     CDS             complement(11184..11738)
                     /locus_tag="IVW29_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7839257.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11991..12266
                     /gene="rpsP"
                     /locus_tag="IVW29_001777"
     CDS             11991..12266
                     /gene="rpsP"
                     /locus_tag="IVW29_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7839258.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12278..12529
                     /locus_tag="IVW29_001778"
     CDS             12278..12529
                     /locus_tag="IVW29_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7839259.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12654..13445
                     /locus_tag="IVW29_001779"
     CDS             12654..13445
                     /locus_tag="IVW29_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839260.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13615..14136
                     /gene="rimM"
                     /locus_tag="IVW29_001780"
     CDS             13615..14136
                     /gene="rimM"
                     /locus_tag="IVW29_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7839261.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14126..14887
                     /gene="trmD"
                     /locus_tag="IVW29_001781"
     CDS             14126..14887
                     /gene="trmD"
                     /locus_tag="IVW29_001781"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7839262.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15023..>15346
                     /gene="rplS"
                     /locus_tag="IVW29_001782"
     CDS             15023..>15346
                     /gene="rplS"
                     /locus_tag="IVW29_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7839263.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAA"
BASE COUNT         5099 a         2776 c         3168 g         4303 t
ORIGIN      
        1 agaagaaagt agcttctctt tgtacatata tggtttgttt ttatggatgc cgagcttttt
       61 tataagataa ggctaccgcc cataacgacc caaggatttg tggaactaag taaatacctg
      121 caagaagcaa gatcagctgg gtcggtttta aaggatgata gaagtaacgc cattcagtaa
      181 aattggcgca aaataaatag gaagtgacag tagatataat attggcaatg aataaagata
      241 taatacgcct gttcctttga aaataatatc cagcaaaaag agataaaaat atcaatgtga
      301 tgaacccgct tacggaatga taacgaaaat caagcagtaa ggcgaaatgc acataaggaa
      361 caaaagataa aagcaacgga aggtttttcc agcaagtttt tatcatcttt catcaccacc
      421 taacaagtgt tattattttg ttagttttat tgtaaattga atcctgcaat aatcaattac
      481 tttttcatta tttctagatt aaatgaataa actctcatgc cattatctct aaaaaaaaag
      541 tttttccccc taattttttt agatagaaaa ttgtatagta tgatgagaga aaaagtagtt
      601 tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat ttttttggtt
      661 ttttcctatc gttttgctac cttttacatg ggtaaatgct gttgtacaag gtgcggagat
      721 attaggcctg atccaaggaa gcggatttgt ggtttctagg aattttccag ctttgactgt
      781 tttctattta gtgataagtg taggtctctt attaccaaaa tctctgatca atcactacaa
      841 ttggcagctg ctttactttt cttgcttatt agtatttaca tctatgtttt ctttgctgcc
      901 tcgcttgatt atcggcccta atggtttgag cgagagtgga ttgaaggatt cagcttattc
      961 ctccatgtat ttcttgacga tcaaaccatg gttctattta gtaattgcct catttattct
     1021 tgcaacattt ttttacttct ggacggaaat caggataaaa agtgaacgat ctgtatctat
     1081 aaaaaataac tattgatggt ggatcatcag taaaaaagca tatgagctgc gtaaaatgca
     1141 tgtttctcat atgctttttt tattattatg taaagaatgc gaaaaacgtt tacatgattt
     1201 ttacataact taactatatt tttacagcaa ccatacgagg gatttacagt cgactgataa
     1261 actttagttg taaataagaa aggagcaata aaatgaaaaa agtattgttt actctaggta
     1321 tgatgggaat tcttttagcc ggatgcggct caggatcagg aaatacagat ggctctgcca
     1381 cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag
     1441 cattacagcc gttagtcgat gcagcacaag aaagctttgt acaagaaaat ccaaattatc
     1501 aaatctctgt acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag
     1561 ttactatcgg aaactctgat gtatttgcag aagaaagaga cggagttgat gcttcaaaat
     1621 tagtggatca taaagtagca gtggtcggga tggcgccaat cgtcaataaa gatacagatg
     1681 tcaaagatat caccaaacag gaattgattg atatctttac aggaaaaatc actaactgga
     1741 aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta
     1801 cccgcgcgac attcgaaaaa tggggactgg atggtgctac ccctgtgcag tcccaagaac
     1861 aagattcatc aggaacagtt cggcagctgg taagtcaaac accaggagca atcagctatt
     1921 tagctttctc atatctcgat gattccacac aagcactaag tatcgacggt gtagaaccaa
     1981 aagaagagaa tgtagcagat aacagctggg gaatctggtc atatgaacat atgtacacga
     2041 acggaaaacc ttcccctgaa gtccaaaaat tcttagacta catgatgaca gaagaaatcc
     2101 aagaaggacc tgtcaaagaa ttgggctatt taccaatcac aatgatggaa gtagaacgtg
     2161 atcacgaagg aaatatcaaa taactagcag ataaacatgc cacggtagtc gttactatta
     2221 aggaaaaatc aactatgttt cccacatata catttttcct ttaattgtga aaaatagttc
     2281 ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattaaac accgtttatt
     2341 cgaaaaaaag agctacgaca tagaactgtc gtagctcttt ttttgctata tgacaggaat
     2401 gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta tactggatag
     2461 gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg aagaataaat
     2521 ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa gtcatccgtc
     2581 tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga atagaaaaag
     2641 gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa
     2701 acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt tgtttagtag
     2761 aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga atcgccattt
     2821 ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat acagcaaaag
     2881 tcaatgtcat acgggacggg atagaacaaa caatagatat agaagacctt gtaatagatg
     2941 acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc gtggaaggaa
     3001 aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc aaagaaatag
     3061 gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc cgattagaca
     3121 aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca atgggagaag
     3181 gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt gggatcatca
     3241 ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga aacacgatca
     3301 aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa ggactgtact
     3361 tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa agagttcttt
     3421 tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt gtagataaaa
     3481 caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct aaaaaacaag
     3541 aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca aaagcgatgg
     3601 caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc actgatcaat
     3661 cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta gcgttggcag
     3721 acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg
     3781 cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg
     3841 atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc tatattctga
     3901 ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa aaacagcaag
     3961 tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta gctgtccaag
     4021 cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa gaagaatatg
     4081 aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa aagaaaatct
     4141 tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4201 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4261 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4321 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     4381 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     4441 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     4501 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     4561 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     4621 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     4681 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     4741 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     4801 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     4861 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     4921 aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga gaaaattgac
     4981 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5041 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5101 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc attaaggagt
     5161 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc
     5221 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5281 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     5341 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     5401 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     5461 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     5521 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     5581 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     5641 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     5701 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     5761 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     5821 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     5881 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     5941 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6001 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6061 ttcagtaacg tatataattg ctcttctgtg gtagtacacg cccgatacat cttactcatt
     6121 tgttcgctca acgcattaat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6181 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6241 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6301 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     6361 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     6421 acatataaaa gatcctcatt caaagtagta cttttgcgca aagcgctacc taggttccca
     6481 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     6541 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     6601 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     6661 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     6721 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     6781 agaaagaaga gacgttcata aatttttttc catttcatcg ttttggttcc tgaaacttat
     6841 agccaaatcc tctaatagtc accaagtact ttggatgttt tggatctcgc tcgatttttt
     6901 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     6961 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7021 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7081 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7141 ttgattgctt acgaggttca atccgtctga atattgcttt catacgggct aatacttcgc
     7201 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7261 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7321 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     7381 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     7441 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     7501 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     7561 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     7621 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     7681 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     7741 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     7801 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     7861 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     7921 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     7981 cccattcgat ttatgacgga agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8041 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8101 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8161 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8221 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8281 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt ttctaattat
     8341 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     8401 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     8461 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     8521 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     8581 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     8641 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     8701 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     8761 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     8821 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     8881 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     8941 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9001 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9061 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9121 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9181 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9241 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9301 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
     9361 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac gagaaaaaag
     9421 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
     9481 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
     9541 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
     9601 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
     9661 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
     9721 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
     9781 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
     9841 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
     9901 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
     9961 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10021 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10081 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10141 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat atcaaaaaag
    10201 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10261 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10321 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    10381 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    10441 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    10501 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    10561 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    10621 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    10681 agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    10741 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    10801 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    10861 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    10921 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    10981 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11041 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11101 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11161 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11221 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11281 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    11341 acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    11401 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    11461 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    11521 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    11581 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    11641 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    11701 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    11761 atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact tactgacaaa
    11821 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    11881 tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    11941 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12001 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12061 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12121 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12181 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12241 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12301 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    12361 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    12421 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    12481 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    12541 gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga aatgattatt
    12601 tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag ccaatgagaa
    12661 agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg gttactcaag
    12721 aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa
    12781 gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat aatgagaaaa
    12841 agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct ctagatgtac
    12901 cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt tattctattt
    12961 tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat caagaaacgc
    13021 ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca atccaagttt
    13081 tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct cgaatcacag
    13141 ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga aactggcgta
    13201 ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct aaagaaatcg
    13261 tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa attactgttg
    13321 atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct
    13381 atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct atgaagaggt
    13441 tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag gaagtaatca
    13501 aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga aaactagaat
    13561 tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata taagttgact
    13621 gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt
    13681 atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct aacactattc
    13741 caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt
    13801 gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata tcgggatggc
    13861 atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt ctattaccat
    13921 gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt aaaagaaatc
    13981 ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg
    14041 cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta
    14101 gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat ttccaagaat
    14161 gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag gattattaga
    14221 tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag tagatgatta
    14281 tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg ataacatcca
    14341 agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg acccagctgg
    14401 taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact tagtattcat
    14461 ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag atgaagtttc
    14521 cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga tcgatgcaac
    14581 agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg attcttattc
    14641 aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag ggatgaaagt
    14701 accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat taaaagaatc
    14761 actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat ggacacctca
    14821 aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc
    14881 caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag tattttatgg
    14941 tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc ataagaatat
    15001 ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa aagaacaatt
    15061 acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg caaaagttgt
    15121 tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc
    15181 tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac
    15241 attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg
    15301 tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgct
//
LOCUS       DACCVU010000064        15026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCVU010000064 DACCVU010000000
VERSION     DACCVU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.064"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1165
                     /locus_tag="IVW29_001783"
     CDS             <1..1165
                     /locus_tag="IVW29_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839264.1"
                     /translation="INFIVYLIYYLLMVIMAVIAQDTLHASLSQAGFASGIYIIGTLL
                     ARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVSTA
                     TNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVFF
                     VTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFLS
                     SYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLLS
                     VTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVGP
                     YLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1313..1678)
                     /locus_tag="IVW29_001784"
     CDS             complement(1313..1678)
                     /locus_tag="IVW29_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7839265.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1825..2598)
                     /locus_tag="IVW29_001785"
     CDS             complement(1825..2598)
                     /locus_tag="IVW29_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7839266.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2772..3632)
                     /locus_tag="IVW29_001786"
     CDS             complement(2772..3632)
                     /locus_tag="IVW29_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7839267.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3632..5050)
                     /locus_tag="IVW29_001787"
     CDS             complement(3632..5050)
                     /locus_tag="IVW29_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7839268.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5107..5556)
                     /locus_tag="IVW29_001788"
     CDS             complement(5107..5556)
                     /locus_tag="IVW29_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839269.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6001..6264)
                     /locus_tag="IVW29_001789"
     CDS             complement(6001..6264)
                     /locus_tag="IVW29_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7839270.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6363..7658)
                     /locus_tag="IVW29_001790"
     CDS             complement(6363..7658)
                     /locus_tag="IVW29_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7839271.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7655..8926)
                     /locus_tag="IVW29_001791"
     CDS             complement(7655..8926)
                     /locus_tag="IVW29_001791"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7839272.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9091..9960)
                     /locus_tag="IVW29_001792"
     CDS             complement(9091..9960)
                     /locus_tag="IVW29_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7839273.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10252..11862)
                     /locus_tag="IVW29_001793"
     CDS             complement(10252..11862)
                     /locus_tag="IVW29_001793"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7839274.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13055..14395)
                     /gene="glnA"
                     /locus_tag="IVW29_001794"
     CDS             complement(13055..14395)
                     /gene="glnA"
                     /locus_tag="IVW29_001794"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7839275.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14423..14815)
                     /locus_tag="IVW29_001795"
     CDS             complement(14423..14815)
                     /locus_tag="IVW29_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7839276.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4230 a         3227 c         2585 g         4984 t
ORIGIN      
        1 gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg cagtcattgc
       61 gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga tctatatcat
      121 cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg gtcggaagtt
      181 taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc tttatacgcc
      241 aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg gtacagtttc
      301 tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag gggaaggcat
      361 caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt taggaatgat
      421 tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac tggtcttctt
      481 tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg aagaacaaaa
      541 aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac ttttcattac
      601 aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt tatcttctta
      661 tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt
      721 catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag aacaatacgt
      781 gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg taacgacgaa
      841 tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta cgtttatgtc
      901 aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc
      961 tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga tggggatgct
     1021 aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc cattgatatg
     1081 tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg gcgaattgat
     1141 tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc gttcagatct
     1201 gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt aattaaacat
     1261 ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg atttattcca
     1321 ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag aaccattctg
     1381 ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact gtatcattca
     1441 taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg atcgtcgctg
     1501 cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc atgactacaa
     1561 taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct gtcagcacga
     1621 cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca tgtttcattt
     1681 tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa ccttttcatt
     1741 attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa atgaggaagc
     1801 cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt gttcctttac
     1861 tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga tttcatgaaa
     1921 agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta aaacggcaag
     1981 ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat gagtttctgt
     2041 tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg cataatatga
     2101 caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa caattgcccc
     2161 tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg tgacgatgcc
     2221 attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt gtaaagaggt
     2281 tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag ctgtatctgc
     2341 aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt aaggcacgtc
     2401 aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca atccactttt
     2461 ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta actcgatcaa
     2521 atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct gctgtctatt
     2581 ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag gaaaaaagag
     2641 gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa aaactacttc
     2701 tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct cttgaccaca
     2761 catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc cattttatgt
     2821 ttgaccactt cttgtacctt tttattagct gctggataaa ttgcattcgg ctcgtacatc
     2881 gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt aagatctgag
     2941 ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc gtccggatta
     3001 cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc tttcagtaag
     3061 tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt tccaattgct
     3121 acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc aggatctgta
     3181 taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt tccaagctca
     3241 gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt aatctgtaca
     3301 ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc gtttcgaatc
     3361 gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat cggcagttta
     3421 cttgtataag cataggctct tactgccgca acaaactcat cacttaaata ggcgatttca
     3481 tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc gatgattgct
     3541 ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt ctctttagcc
     3601 actttcaata actctttcat tgtatataac attacgcttc cacctcattt gttagatttt
     3661 catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat agtccaagca
     3721 taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt aaattgttca
     3781 taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa gtaactccta
     3841 ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt atcacgcgga
     3901 tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg cctaaaatcc
     3961 agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat aataaagtgg
     4021 ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca atcggtgcat
     4081 ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt cgatttattg atcggcccac
     4141 ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg gatgtatttc
     4201 ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt tgttcaatca
     4261 caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca atcagctgat
     4321 aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca cagatatacc
     4381 ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta gaggccagta
     4441 atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta gcaatccccg
     4501 cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt gaaaatgcag
     4561 ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca aaggcaaaac
     4621 tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc acaccattca
     4681 tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt tcattagaca
     4741 caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt tctttgattg
     4801 ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat tcgatttctt
     4861 tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat aactgatttt
     4921 ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt gctgttactt
     4981 ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca gttacaccaa
     5041 cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac aaagactcta
     5101 ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg cttcttttat
     5161 tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa tctctagatg
     5221 catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg gcatttcatc
     5281 tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg gttttgtcac
     5341 atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg agatctcctc
     5401 ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat ttgttttttt
     5461 caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg gatcgaaaac
     5521 aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc ccacaaaaaa
     5581 tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc gttcaatatt
     5641 atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa aatgaacgat
     5701 ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg aaaggaaagc
     5761 ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct tcatttcgtt
     5821 aatataaggt gtttttatca agtggataat taaaactata tactagtttt tcattgtttt
     5881 gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca
     5941 taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc agcgttttta
     6001 ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt ccacacgtcc
     6061 gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag aagttacttc
     6121 gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt gtgaagtttt
     6181 tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta ctgggtggta
     6241 atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc catttgctga
     6301 accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag tcaaggttgc
     6361 aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc ttgaaattct
     6421 ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat
     6481 tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa ttcttcactt
     6541 ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc tgggaagatc
     6601 cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc tttgaattct
     6661 tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc taaaatcgtc
     6721 aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg tttatacaat
     6781 gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac taagttatag
     6841 gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt atcttcgact
     6901 aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg cggctgcaga
     6961 tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc
     7021 ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc ggtgatacca
     7081 tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac gatcaatcca
     7141 cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag tctttgcggt
     7201 gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga
     7261 cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat caaaccataa
     7321 taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc atttctcaaa
     7381 ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg tccataattg
     7441 attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc ttccatataa
     7501 taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc ctttttattc
     7561 atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc ttttaacgtt
     7621 ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact tcctctatca
     7681 tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa ccgcgtaaga
     7741 tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca atcactaaac
     7801 aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta tttggaccat
     7861 tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct agttccggca
     7921 catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct gtagtcatca
     7981 atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa ggataggtca
     8041 tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct tcgctgattt
     8101 tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt tcaaagatca
     8161 catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag gttcctgctt
     8221 cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact ccttcgatac
     8281 ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt aaagttgcca
     8341 catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct tttgctttta
     8401 cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg aaaatccgat
     8461 tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca tttgtcactt
     8521 cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg cctaaataac
     8581 agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt cccataaaat
     8641 aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat acgacttctc
     8701 gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg atctctaaaa
     8761 tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc actggggaat
     8821 cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta
     8881 cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt ttccaccttg
     8941 tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa aaagaaacga
     9001 gcatggtctg ccgttttaag taaaaaagaa tggagagcga cttcaaaagt cgttctccat
     9061 tcctatatat cgtatctgtt atgtggatgc ttacgctttg ttagctgaac cgaaccagtc
     9121 gatatgagat ttagcatctt caacgattgc tgctttacct ggagctaata atttacgagg
     9181 gtcgaatcct ttaccttctt tatctttacc agcttcgata tatttacgag tagctgcagc
     9241 aaatgataat tggaattcag tgttaacatt tacttttgag atacccattg agatagcttt
     9301 ttcgatttga tcttgaggaa tacctgatcc gccatgtaat actaaaggca tgtctggacc
     9361 tacagcatca gcaattgctt gtaagtggtc aaaagctaat cctttccagt tttctggata
     9421 ttgaccatgg atgttaccga taccgcaagc taagtagtcg atacctgtag caaccatttg
     9481 tttgcattct tcaatatcag ctaattcgcc gttaccgatg attccgtctt cttctccgcc
     9541 gattgaacca acttcacatt caactgagat accttttgca tgtgcttttt caacaacgtc
     9601 tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt
     9661 atagcctaat tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta agtggatagc
     9721 aactggaaca gtgatgttca ttgcttcaac taaatcagtg attaaatctt tacagatttt
     9781 gtaaccaccc atgtatttag ctgcacccat tgatgtttgg ataagtactg gtgcttgttt
     9841 tgcttcagcc gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc
     9901 gacagcgtaa ccgcctttac gagcagcttt taaaaattca gctcctgata ctactggcat
     9961 aataaaattc ctcctaagtt ttgtaaaact aaatttgtta agacaattct taaccaacga
    10021 ccctatttta ccaaacattt cggtagtttt ctactaaaat caatgaaaat gaaaattttc
    10081 aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa aagtcttttg
    10141 ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag tgacaagaaa
    10201 attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa attattttgt
    10261 ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg
    10321 acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct ctggcagctc
    10381 tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc cttgttcttc
    10441 aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt cttgcacaac
    10501 atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac atgggtataa
    10561 tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca ttaaatcaat
    10621 aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta atccaacgac
    10681 gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta aaaacggaac
    10741 gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc gatcaccaaa
    10801 tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat tttctctcgt
    10861 taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga aaccagaatg
    10921 tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt taccgacaag
    10981 cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt tccattctgt
    11041 catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat ccattccttg
    11101 tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact cgataactgc
    11161 ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag aaacaggttg
    11221 ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct cttttacact
    11281 gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa tcaatgttgt
    11341 atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa gtgcttcaag
    11401 gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga tcacatctga
    11461 atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa tatgaggaat
    11521 aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa cttcagaata
    11581 gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa aacgttcgta
    11641 gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt ctccgtgctg
    11701 atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt ggatagttac
    11761 ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc cttttccgat
    11821 tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa aaactccttt
    11881 ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc ttcaataaga
    11941 atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca aggtgtataa
    12001 tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt acaatttttt
    12061 ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca aaaaaaattt
    12121 aacccacata ataagcttga tataaacata gctaacgtta aacctaagca aatcagaaaa
    12181 tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa cattatgata
    12241 gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag tttatatagc
    12301 ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt aaaaaacgta
    12361 gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa aaacaaaaca
    12421 attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg tagggattca
    12481 ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct
    12541 tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccatc
    12601 aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat tgtaggctca
    12661 caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    12721 aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa acgtcttttt
    12781 caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag ttcagccaat
    12841 tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc agggcagctt
    12901 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    12961 acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg tcaaaacgct
    13021 gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc
    13081 tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa
    13141 aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt
    13201 tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct
    13261 catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac
    13321 cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa
    13381 ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg
    13441 aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt
    13501 tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac
    13561 cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa
    13621 catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa
    13681 ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa
    13741 cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa
    13801 tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt
    13861 ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa
    13921 aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata
    13981 agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt
    14041 tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat
    14101 agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc
    14161 aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa
    14221 tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa
    14281 cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt
    14341 tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat
    14401 ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat
    14461 aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa
    14521 cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt
    14581 cgtatacacg tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa
    14641 gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat
    14701 gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca
    14761 taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac
    14821 gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg
    14881 catatgttag gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga
    14941 acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt tattatactg
    15001 tactatcctt tattagtgtt tttatt
//
LOCUS       DACCVU010000065        14600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCVU010000065 DACCVU010000000
VERSION     DACCVU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.065"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            620..1483
                     /locus_tag="IVW29_001796"
     CDS             620..1483
                     /locus_tag="IVW29_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7839277.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1612..3234)
                     /locus_tag="IVW29_001797"
     CDS             complement(1612..3234)
                     /locus_tag="IVW29_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7839278.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3483..4004
                     /locus_tag="IVW29_001798"
     CDS             3483..4004
                     /locus_tag="IVW29_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839279.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IVW29_001799"
     CDS             complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IVW29_001799"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7839280.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6085..6903
                     /locus_tag="IVW29_001800"
     CDS             6085..6903
                     /locus_tag="IVW29_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7839281.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7006..7203)
                     /locus_tag="IVW29_001801"
     CDS             complement(7006..7203)
                     /locus_tag="IVW29_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839282.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7372..7950
                     /locus_tag="IVW29_001802"
     CDS             7372..7950
                     /locus_tag="IVW29_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7839283.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8995..9498)
                     /locus_tag="IVW29_001803"
     CDS             complement(8995..9498)
                     /locus_tag="IVW29_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7839284.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9857..10882
                     /locus_tag="IVW29_001804"
     CDS             9857..10882
                     /locus_tag="IVW29_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7839285.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11205..12083
                     /locus_tag="IVW29_001805"
     CDS             11205..12083
                     /locus_tag="IVW29_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839286.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12559..12675
                     /locus_tag="IVW29_001806"
     CDS             12559..12675
                     /locus_tag="IVW29_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7839287.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13603..13941
                     /locus_tag="IVW29_001807"
     CDS             13603..13941
                     /locus_tag="IVW29_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839288.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14035..14265
                     /locus_tag="IVW29_001808"
     CDS             14035..14265
                     /locus_tag="IVW29_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839289.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4633 a         2433 c         2781 g         4753 t
ORIGIN      
        1 cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa
       61 gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg
      121 cagagcagct ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
      241 gagctaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg
      421 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
      481 cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac
      541 cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa
      601 cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata
      661 ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca
      721 agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa
      781 aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg
      841 ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg
      901 ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga
      961 gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc
     1021 tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa
     1081 ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg
     1141 taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac
     1201 ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat
     1261 ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta
     1321 tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca
     1381 gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg
     1441 ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct
     1501 gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca
     1561 tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca
     1621 taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc
     1681 agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat
     1741 aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt
     1801 caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt
     1861 agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac
     1921 attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa
     1981 gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag
     2041 atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc
     2101 ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt
     2161 tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga
     2221 tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa
     2281 gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg
     2341 ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga
     2401 ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc
     2461 ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa
     2521 atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca
     2581 tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat
     2641 caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag
     2701 caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc
     2761 accagttaat tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag
     2821 taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa
     2881 ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt
     2941 ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata
     3001 gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt
     3061 aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc
     3121 tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc
     3181 atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt
     3241 tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta
     3301 aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa
     3361 aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt
     3421 ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga
     3481 aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac
     3541 ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct
     3601 ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag
     3661 agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat attctagttg
     3721 gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag
     3781 tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa
     3841 gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt
     3901 tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt
     3961 ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat
     4021 aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca
     4081 tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag
     4141 aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct
     4201 tttcatataa ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt
     4261 ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc
     4321 gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa
     4381 aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc
     4441 caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt
     4501 cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa
     4561 gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac
     4621 ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac
     4681 cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca
     4741 ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt
     4801 cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat
     4861 cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc
     4921 agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat
     4981 caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga
     5041 tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga
     5101 caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc
     5161 cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag
     5221 cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg
     5281 ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta
     5341 gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag
     5401 gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt
     5461 tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca
     5521 tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga
     5581 aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat
     5641 ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc
     5701 cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga
     5761 cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca
     5821 taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa
     5881 gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca
     5941 atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact
     6001 aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag
     6061 atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag
     6121 caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt
     6181 tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt
     6241 gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa
     6301 aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta
     6361 tatatggcag ataaaacact gccccatatg cattttggac cacaacatga agcagaagga
     6421 ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt
     6481 cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca
     6541 gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa
     6601 ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt
     6661 tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc gctactagta
     6721 acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat
     6781 gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt
     6841 gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga
     6901 taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta
     6961 tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt
     7021 tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag
     7081 aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa
     7141 gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg
     7201 catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt
     7261 aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg
     7321 ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa
     7381 aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta
     7441 gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt
     7501 tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta
     7561 tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt
     7621 gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct
     7681 ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca
     7741 aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat
     7801 tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta
     7861 gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca
     7921 agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa
     7981 atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat
     8041 gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat
     8101 ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt
     8161 attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa
     8221 aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa
     8281 tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta
     8341 agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata
     8401 tacaacagaa atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt
     8461 ggaataaaaa aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac
     8521 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
     8581 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
     8641 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
     8701 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
     8761 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
     8821 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
     8881 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
     8941 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
     9001 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
     9061 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
     9121 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
     9181 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
     9241 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
     9301 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
     9361 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
     9421 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     9481 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     9541 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     9601 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     9661 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     9721 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     9781 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     9841 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     9901 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     9961 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
    10021 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
    10081 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
    10141 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
    10201 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
    10261 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
    10321 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
    10381 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
    10441 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
    10501 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
    10561 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
    10621 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
    10681 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
    10741 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
    10801 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
    10861 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
    10921 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
    10981 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
    11041 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    11101 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11161 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11221 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11281 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11341 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11401 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11461 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11521 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11581 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11641 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11701 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11761 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11821 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11881 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11941 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    12001 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    12061 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12121 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12181 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12241 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12301 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12361 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12421 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12481 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12541 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12601 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12661 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12721 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12781 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12841 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12901 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12961 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    13021 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    13081 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13141 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13201 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13261 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13321 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13381 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13441 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13501 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13561 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13621 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13681 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13741 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13801 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13861 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13921 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13981 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    14041 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    14101 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14161 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14221 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14281 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14341 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14401 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14461 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14521 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14581 atttctcctg gttgggaaaa
//
LOCUS       DACCVU010000066        14597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCVU010000066 DACCVU010000000
VERSION     DACCVU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.066"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(243..1139)
                     /locus_tag="IVW29_001809"
     CDS             complement(243..1139)
                     /locus_tag="IVW29_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7839290.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(1132..1812)
                     /locus_tag="IVW29_001810"
     CDS             complement(1132..1812)
                     /locus_tag="IVW29_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839291.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1813..2595)
                     /locus_tag="IVW29_001811"
     CDS             complement(1813..2595)
                     /locus_tag="IVW29_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7839292.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2588..3505)
                     /locus_tag="IVW29_001812"
     CDS             complement(2588..3505)
                     /locus_tag="IVW29_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7839293.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3669..3992)
                     /locus_tag="IVW29_001813"
     CDS             complement(3669..3992)
                     /locus_tag="IVW29_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839294.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4780..8007
                     /gene="fss3"
                     /locus_tag="IVW29_001814"
     CDS             4780..8007
                     /gene="fss3"
                     /locus_tag="IVW29_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7839295.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            8120..8434
                     /locus_tag="IVW29_001815"
     CDS             8120..8434
                     /locus_tag="IVW29_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7839296.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8447..8821
                     /locus_tag="IVW29_001816"
     CDS             8447..8821
                     /locus_tag="IVW29_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7839297.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8831..9166
                     /locus_tag="IVW29_001817"
     CDS             8831..9166
                     /locus_tag="IVW29_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7839298.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9249..10589
                     /locus_tag="IVW29_001818"
     CDS             9249..10589
                     /locus_tag="IVW29_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7839299.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10667..11341
                     /locus_tag="IVW29_001819"
     CDS             10667..11341
                     /locus_tag="IVW29_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7839300.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11574..12008
                     /gene="dcm"
                     /locus_tag="IVW29_001820"
     CDS             11574..12008
                     /gene="dcm"
                     /locus_tag="IVW29_001820"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7839301.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            12009..12716
                     /locus_tag="IVW29_001821"
     CDS             12009..12716
                     /locus_tag="IVW29_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7839302.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            12706..12996
                     /locus_tag="IVW29_001822"
     CDS             12706..12996
                     /locus_tag="IVW29_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839303.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13255..14439
                     /locus_tag="IVW29_001823"
     CDS             13255..14439
                     /locus_tag="IVW29_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7839304.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14454..14573
                     /locus_tag="IVW29_001824"
     CDS             14454..14573
                     /locus_tag="IVW29_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7839305.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4675 a         2675 c         3048 g         4199 t
ORIGIN      
        1 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
       61 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
      121 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
      181 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
      241 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
      301 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
      361 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
      421 gtttgcgttc ctggtttgat aggattggcc actgttaatc gaatgaatgg atcctctttt
      481 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
      541 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
      601 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
      661 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
      721 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
      781 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
      841 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
      901 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccaa ccatgtattc
      961 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
     1021 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
     1081 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca tttaatcaaa
     1141 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagcttccc
     1201 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
     1261 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
     1321 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
     1381 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
     1441 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aaaagttctt cgatatcaaa
     1501 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
     1561 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
     1621 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
     1681 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
     1741 aagaacctcc tccagtaagg aatgaacagc ttcatcgtct tcgataatca aaatagtttc
     1801 cagcaggacc acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
     1861 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
     1921 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
     1981 gcctttgatt tttgcgaaaa caccgggaat gaaggccgtt cccatgatat aaagaaaaga
     2041 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
     2101 tgtccctaat gtcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
     2161 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaagca tggctagtcc
     2221 cgtgatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
     2281 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
     2341 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
     2401 ttctaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
     2461 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
     2521 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
     2581 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
     2641 gcccaactag gtgaatacca tccacaggaa cttttgcttc taccaatgct tgattgatgg
     2701 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgt acaaaataat
     2761 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
     2821 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
     2881 cttcatgcaa aataccatag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
     2941 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
     3001 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
     3061 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
     3121 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
     3181 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
     3241 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
     3301 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
     3361 tagacttacc cgctccattt tccccaatca atccatagat cattccacgt ttgatttcga
     3421 ttgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
     3481 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgatga gtgaagtata
     3541 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
     3601 aatgtcaaaa aaaacaatcg gacaatacgt gtctttccta ttatcaggca gtttgttata
     3661 cgcttgtatc agtttcctga ttctttcttt tcgaccaaag tcgtttctct gtattcctct
     3721 tcactgaaaa tttttgcttc tttcagatca agcttgagta attttaccag accggttata
     3781 aggataacta tagcaagact ccctcccaga aaaattagac tgtctcgcag acgtggttgc
     3841 gttgtcttcc aatcaaagag cgcaccaaac agtgctaatc caagtggact ggccaccatg
     3901 gtaatcgttg ttatcgttga cataagtcgt ccttgtatat gcgttggaac ttctcgctga
     3961 agccagacca tataaaaagc aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa
     4021 taatacatgc cgtttgggat ttacaattag ctaccaaata agaggttatg acaaaagact
     4081 tttcacaatc ccttcttccg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     4141 acaatattta tcaaacatga gaagcaattt ttgtaaattc cttcttattt cttgaagctg
     4201 accacttctg tcacaacctc agaagcggat aaataatatt gtttgatgat actttatctt
     4261 tttcccgttt gtttatagat cgaatgatat tatagaccaa gaatcctacc tattaacata
     4321 gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag tgccagaaaa gttaggatta
     4381 aggtaactgc gctcagcatt gcttcgccca gtacgattct cgtaactcgt gaactcgaac
     4441 gataatcgca attaacgttt tgagaactgt gaagctttac gggctttctt aagacctggt
     4501 tttttcttcg tatcattgaa taagtttgaa aaaccttgat ttataaaggt tttcccgtaa
     4561 atatatttta ttatatttga ttaaatttga aatgtttgtg ttctaaaatg tgttctaatt
     4621 taaaggcagt atatttcgta ttttagatta aatttttagg ctcattcatt ctcaaatcca
     4681 ttctattcag tcaataaaaa ttttaaaaag actttataaa caaagatgtt caagcgagca
     4741 tcttattttt ttgtgcttaa tttgaaagga gaacatttca tgtataaaaa acaaaaaaag
     4801 aaattatgtg tattaactat tttgagtttg gctgttcaag caattgttgg agcaattgct
     4861 cctacgatta cttttgctga tgaaattacc catccacaaa cggtaaccgt agaactagat
     4921 ttagcacatc aatatgctgt tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc
     4981 actgttccgc attatgcagt gtacaacggc tttaaacaag atattttctg tattgaacca
     5041 ggagtcccga tttacaatga gttcactcct ggctatgaga aaaacccgtt accagatatg
     5101 tccgataaag ccaaattagt ttccgtttta tggaaaaaag taggtacaga tgcagatact
     5161 catattgtgg cacagaagat gatctggcaa gaagttaatg ggtataccct tcactcaata
     5221 aaacgatcag atggtagtgc cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc
     5281 attgacgatt atcagaagaa accaaccttc cataatagta cggctaaaac agtattaggg
     5341 caatcgacta ctgtgacgga tacgaataat ctgaatttgt cagagttcga tgaggttgtg
     5401 gaaaatgccg caaacattga ttatcgtgtg aatggtaatc agttgataat caccccaaat
     5461 gccaattcaa aagaaagtgg tgtgctgacg cttaagaaat ctgctggtac cggaacacct
     5521 gtcgcctata aaaaggctgg ccaacagact ttaatggccg gggcgattga taaacctaat
     5581 acctacacaa tcaaaattga tgtggaaacg gagggttctt taaagatcaa aaaagtcgat
     5641 aaagaatcgg gtgatgttgt accaggaacg gttttccatt tagactttgg aaaaacttta
     5701 cctgcaaaag acgtgactac tgacaaagaa ggcattgcta cattggatga gattcctcat
     5761 ggtacaaaag taaccattac cgaaaaatca gtaccggcgc cttatacgat tgataccacc
     5821 cccatgacta ccaccattaa agcaggcgaa accatttacg tgacttcaaa aaacgcacgg
     5881 gaaaaagggc aaatcattct ggataaaagc ggagttgaaa cagggagtga tctttggaat
     5941 gacaattatt ccttagctgg taatacgttt gccattcgca aagacagtcc aaccggtgaa
     6001 atcgttcaag aaatgaccac ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct
     6061 aatgcgttgg aattgggaac ctattatgtg accgaaacta aagctagtca tgggttcgtg
     6121 aatacgttca aacctgtaaa agtcgaattg aagtatgcca atcaaaccgt agcccctgtt
     6181 accagtaacg taaaagggca aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa
     6241 gacaaagata ctggggataa agcacaaggt aaggcagtct ttgagggtgc tgaatacacc
     6301 ttgtttactg ctaaggatgg aaaagctgtt aaatggagtg aggcttttaa accagagtta
     6361 gcgaagggaa cgaaaggttc tgatgaaacg gtcactttga ctttagacga aaagaaccaa
     6421 gttgccgtta aacatttagc tatcaacgag tattattgcc aagaaacaaa agcgcccgaa
     6481 ggatatagcc ttgatgaaac aaagtatcct gtatccatca aaaaggtgga tgatagcgaa
     6541 aaaaatgccg tgattacccg aaatgttacg gcaaaagaac aagtgattcg ctttggcttt
     6601 gatttcttta aatttgctgg atcagctgcg ggcactaccg aaactggctt caatgatctg
     6661 acctttaaag tgtcgccatt ggaagggacc aatgaaatca caggtgccga agatgaagcg
     6721 accacagctt ataacgagca attagggttt gatggttatg gcaagtttga aaatcttcct
     6781 tatggggatt atttacttga agaagtagaa gccccagaag gttttcaaaa gattacgcca
     6841 ttagaaatcc gttctacttt taaggaaaac aaagaagact ttgtgaagag tgagtatgtc
     6901 tttaccatta ccgaacaaga ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag
     6961 aagctgacga acaaagcatt ttctgttagt ttgaaccgtt taatgctcta tgatttgcct
     7021 gaggaagaag atagtttaac ttctcttgcg acttggaaag acggaaacaa agaattaact
     7081 tctctggatt ctaccgagct agttgataaa ttgagttaca atctacatga aatcaaagaa
     7141 gactggtatg tggtagcgca agccattgat gtggatgcga caaaagctgc ccaagaaaaa
     7201 gatgaaaaag ccaaaccagt ggtgattgca gaaacaagcg caacgttggc gaacaaagag
     7261 aaaacaggaa cttggaaaat tcagcataaa ttaaccgccg aacaagtttt aaataaaact
     7321 attgtcttat tcaactatgt gtatgaaaat aaggaagctt ttgaagcagg cgataagcca
     7381 gtagcgaagg atgttagctt gaataaccaa gcccaaactg tcagttgtac agtggaacac
     7441 catgtttcta tccaaacaaa ggcccaccta gaaaacggtt ctcagacctt tactcatggt
     7501 gatgtggtgg atatgtttga tgatgtgtcg attactcatg atgtactgga tggctcaaaa
     7561 gaagctttcg aaacaattct gtatgcttta ttaccggatg gtacaaacaa agaaatttgg
     7621 aaatctggca agattgatta tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg
     7681 aaaaaagtag ataccggaaa atatccagaa ggaaccaagt ttacttttgc ggaaatcaat
     7741 tacgataaag atgggaatat caatggaaaa cacaatgaag atttgaaaga aaaatctcaa
     7801 accttaacac caaaagaagt tccaaccata ctaagtacgc cgaaacaacc ggaaacacca
     7861 actgttccaa gtggctctca agaatctagt cccacagtga agaccttccc tcaaaccggg
     7921 gagaaaaatt ccaatgtttt actgttcatt ggttttactt tgatttttgc gacggcgggc
     7981 tattatttct ggaatcgccg gaactaaggt gatgagatga tacaagtaaa tcctaagaac
     8041 caacgtgcgc gaccgcctcc taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca
     8101 atgaaaagag gaaaagaaga tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt
     8161 tggaaagtta agttatgccg gtgaaggaga aattataaca gaaggatatg gacggaatac
     8221 tacggtgatt ggtcgcagtt atcatttgta ttccagtaag caacgggccg atgatattga
     8281 ggtggtagta gctgcggaag ccggtgaaaa ggattttgat caagaccaac cgctaaaagc
     8341 cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt ttactgatta
     8401 cgtgttgtat gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga
     8461 aggtattgtc gtagattcag gtttaacatt tgggaagttg cgtttttccg cattacgccg
     8521 agaagtacgg aagcaaaatg aggatggaac aattggtaat gaggtaaaag aacgaaccta
     8581 taatttaaaa tcctctgcac aagggcgaat gattcaagtt agtatcccag ctaatgtacc
     8641 cttacgcgag tttgcttatg atgcagaggt agagttggtc aatccaatcg tggataccgt
     8701 tgccaattat gtttttcgag aaggaactac cgttaattgg ttcattaagg cggatgattt
     8761 agtcttgaag cggcagccga atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata
     8821 ggtgaagatt atggaaatta atgttgaaca tattttagat tgtctcgatc aatatggaaa
     8881 aggcgagctg actgaggaac aactcgttaa agcattaacc tctgatgaaa aaatgtttct
     8941 gattatacat caaggtttac ttggagaaaa tagcgacagt gaagaagact ctgatgtact
     9001 agactggtta ggagaagaag agtctttctt tatagttgtc gaagtaaatg aaaacttatg
     9061 tcggcaggca gaatccattt tggaagaaat tggtgtggag atgccggatg ctattgaagg
     9121 ctttttaaat caattggttg aaacgaaaca actcccctcc tcgtagcagt aaatgactag
     9181 cataactttt tgaaattgcc taatgaaatc ctattgggct ttttcgattg gaggtggaaa
     9241 ccatcaaaat gtacaaagga catcgcatac gcgcaggaga tcagcacttg gtttatcact
     9301 ttgttcttgg ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag
     9361 agtttagaca atttgatatt tctaagctat cactttctac tattgaaatc gtttggtcta
     9421 taaaagattt agtatgtctg cttggaagtc tcgctttttc aggagctatg atattgcttt
     9481 atattcactt ttttcttgat cattggcgaa gcttgtggca tcgacaaaag ctagctcgga
     9541 tgattctaga gaaccattgg tatgaagtga aacaaactca aagtgaaggc tttttcaaag
     9601 atttgaatag tagccgaacc agagagacta tcagttactt ccccaaaatc tgttatcgaa
     9661 tgaaagatgg attactgtct attcgcgttc aaatttcact tgggaaatac caagatcagc
     9721 ttttaaaatt ggaaaagaag ttagaaagcg gactgtattg tgagttagta gaaaaggaac
     9781 tcaaagactc ctatgtggag tatactttac tgtatgatat gatcgccaat cgaattggga
     9841 tagacgaagt agtggcagaa aatgggactc tgcgattgat gaaaaatcaa gtatgggctt
     9901 atgattcttt accccatatg ttaatcgctg gtggcacggg tggagggaag acctatttcc
     9961 tcctcaccat tatcgaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca
    10021 aaaatgcaga tttagctgat ttaggtacgg tgatgcctca tgtttattca caaaaggaag
    10081 aaatttctgc ttgtgtggaa gatttctatg aacgtatgat ggctcgtagc aaggcaatga
    10141 aagaaatgtc taactacaaa acaggagaga attatgccta tcttggactt ccacccaact
    10201 ttttaatctt tgatgaatac gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg
    10261 tgattttgaa taagctcaaa caaatcgtga tgttgggtcg tcaatctggt ttctttctga
    10321 ttttggcttg ccaaaggccg gatgcgaaat atttaggaga cggaatccgc gatcaattca
    10381 actttcgcgt ggctttgggt cgtatgagtg aactcggata ttccatgatg tttggggagg
    10441 tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg ttatgtggat acaggtggca
    10501 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttagattcaa
    10561 ttagtaaatt agaaaagatg aatgattgag tgatcttcaa tactgacttt atacttcaag
    10621 taacgtataa tacttttata tagtaggaag aaaggggata attaatatgg aaagtaatga
    10681 ttcttattta gagtatgcaa aagcaaataa ggataagttt atttcttata ttatcaaagg
    10741 gaaatccccc gaagttggac gagacgcagt ttttatggca gggagtcctg gggctggaaa
    10801 atcagaggtt gccattggat tagctgagaa ttatgataac catgtgatta ttgatgcgga
    10861 ttactttcgc acccagtttc ctgattataa tggaaaaaac tctagtctct ttcaaaaagc
    10921 atcttcttgg ttggtagagc aggcatttag atatgtctta gatcatggct attcttttat
    10981 tctggatgca acttttgcaa tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa
    11041 acattttcga gtgacgattt tctatgttta tcaagaccct aaaatcgctt ggaaatttac
    11101 acaagaacgt gaaattgcag agggacgtca tgtgccgaaa aaggcattta ttaacgcttt
    11161 tttcaaatca agagagaaca ttgcaaaagt aaagagtaaa catccagatg tattgcttca
    11221 tatcataata aaagattata cgaataatat ttccgaagtt cattatgcta cggataatct
    11281 agaattattt ttaccactag ggtatagtag gaaagatttg gaggttcaat tacatgactg
    11341 agaaagaaca agtaacgaaa attgtaaaga aatacaataa gagcattgcg gatttatcgg
    11401 aaaatgctac tgcaaaagag ttcaaaacgg tgattaaata tgtagccgat caggccaatg
    11461 aaaaacaacg gaaattagtt ggattaaata aaaaatagta ggacaaatta ataatgtatg
    11521 agccaagagc aaaatgttct tggctttttt ctttgcaatg aatgaggtaa tgtatgagat
    11581 ttttagattt attcgccggt attggtggct ttcgtttagg tatggaacag gcgagccatc
    11641 attgtatcgg tttttgtgaa attgataaat ttgctagacg tagttataaa gcaattcatg
    11701 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagatgag tttattcaat
    11761 ccctcggacc agtggacatc ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg
    11821 gaaagcggca aagattttcc gatactcgag gtactttatt ctttgaaatc gctcggttca
    11881 ccactcttct ccgacctaag cttttattcc ttgagaacgt ccgaggattg ctcaatcacg
    11941 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgacgtgg
    12001 agtggtaggt gcttaactca aaggcctaca ttccccaaag ccgggagcgg atattcctta
    12061 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa
    12121 catctgatca aaacatcaga aatttggtaa atatcaatcc ttccaatcga ggaatgggtg
    12181 gacaagtcta tggttcagat ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa
    12241 tcgcactgcc agcaagggat ggaatctctg tagcaggact attaccggga aagtttgaac
    12301 aaggtaatcg agtttatgaa gttactggaa ccgcccccac cttgtcaacg aaacaaggtg
    12361 gtacaaaaat catgattcga aaaaatgaaa cagaatatca agaggtgaag ccagaagaca
    12421 gtgtgaatct ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacagagtg
    12481 ttcatacctt actaacagga gaccaacaag cgatcgtcac ggatcagtat caaattcgga
    12541 agctaacacc tagagagtgt tggcgacttc agggctttcc cgattgggct tttgatcgag
    12601 attctcaagt caattcagat agtcagttgt ataagcaagc cggaaactct gtaacagttc
    12661 cggtcatttt tgatattgcc agaagattaa aagagttgga tgtacatggt ttatgatgag
    12721 ttgattggag aaatttattg ggtgattgga aagattcagt cagacccaga attagaagaa
    12781 caattgcgac ggttaaaatt tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca
    12841 tatttaatga atgaggagac ggatgctcga attgaaataa atcaagtaat tgcagaattt
    12901 gagcggattg cggaccaagc aaaagaacca gatattcgtc aatacttgtt agaaatgaaa
    12961 gcagagttgg aagtaaatgg tattacagat gagtaaccta aaccaacaca actgactgtt
    13021 aggcgctgac cgcggaggat tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat
    13081 ggacggcgtt agccgaccat gatgtctcac ccccgtatct aacagggggg tacaaaaaca
    13141 caaaaaataa gtaagcaacc attaagaatg cttgttgtat caaagatagt aagtaatttg
    13201 tataggtgtt aactcatttg aaaaagttaa caccttgaat ttggaggtga gaacttggca
    13261 caacggaatt tagattatcg gctattaaaa gaccgacgca acgaatacgg cgtttcacaa
    13321 aataaattgg ctacggcttg tggacttagt cggccgtact taaatcagat agaaaatggt
    13381 ggcgtaaccg catctataaa aaccatgagg aaaatttttg accaactgga aagctttaat
    13441 cccgatttgc ctttgacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat
    13501 gcacgaaaaa ttattcaaga aattctccat ttgaaatttg agtatatgct ccatgaagat
    13561 tatgcctttt actcctatca agaacagtat gtcatgggtg atattgtggt gatgttgtct
    13621 catgaagaag ataaaggtgt gcttttagaa ttaaaggggc gaggttgccg acaatttgaa
    13681 acttttttac tcgctcaaaa gcgtagctgg tacgactttt tcgaggattg tctaaaggcc
    13741 ggtggcgtga tgaaacgctt ggacttggca atcaatgatc gagtggggct attggatata
    13801 cctgagctaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa
    13861 agttatcgtt ccggggaact tttaaaggct gatgaaaagg acggcatggg caatacctta
    13921 tatatcggca gtctcaagag tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa
    13981 tatatcaagt tagggattcc attagaccaa actgagacga aaaatcgttt tgagattcga
    14041 ctgaaaaatg accgtgcttt tcatgcgatt caagatttgt taaaaggtcg tagcattgag
    14101 agcaccacgt tttccattat caatcgttac ttgcgatttg cggataaagt agaaggtaaa
    14161 aggcgaacca actggccctt gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa
    14221 gagattcaat taacttccga gccaaaaccg tataccattg aacgaacctt gaattggcta
    14281 ggccgacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcggaca
    14341 acctatatcc aggatatggt acagaatgca cgcttatcag accgacataa aaagattttg
    14401 gagcaacaaa gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtcatgggaa
    14461 ttgtaaaaga tatttttttg gtatttggtg gcagtatgct tggtgttact gtgatgtgct
    14521 tgatgaatgc aagtgcagca gcggatcgag caatggaaaa gaaaggaaag tgacaagatg
    14581 aatttcggac aaaattt
//
LOCUS       DACCVU010000067        14547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCVU010000067 DACCVU010000000
VERSION     DACCVU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.067"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            2..814
                     /locus_tag="IVW29_001825"
     CDS             2..814
                     /locus_tag="IVW29_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7839306.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            811..1611
                     /locus_tag="IVW29_001826"
     CDS             811..1611
                     /locus_tag="IVW29_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7839307.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1772..3040
                     /gene="rseP"
                     /locus_tag="IVW29_001827"
     CDS             1772..3040
                     /gene="rseP"
                     /locus_tag="IVW29_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7839308.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3108..4817
                     /locus_tag="IVW29_001828"
     CDS             3108..4817
                     /locus_tag="IVW29_001828"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7839309.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5029..9387
                     /locus_tag="IVW29_001829"
     CDS             5029..9387
                     /locus_tag="IVW29_001829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7839310.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9532..10005
                     /gene="rimP"
                     /locus_tag="IVW29_001830"
     CDS             9532..10005
                     /gene="rimP"
                     /locus_tag="IVW29_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7839311.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10028..11203
                     /gene="nusA"
                     /locus_tag="IVW29_001831"
     CDS             10028..11203
                     /gene="nusA"
                     /locus_tag="IVW29_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7839312.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11225..11518
                     /locus_tag="IVW29_001832"
     CDS             11225..11518
                     /locus_tag="IVW29_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7839313.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11515..11826
                     /locus_tag="IVW29_001833"
     CDS             11515..11826
                     /locus_tag="IVW29_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7839314.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11839..14145
                     /gene="infB"
                     /locus_tag="IVW29_001834"
     CDS             11839..14145
                     /gene="infB"
                     /locus_tag="IVW29_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7839315.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14172..14519
                     /gene="rbfA"
                     /locus_tag="IVW29_001835"
     CDS             14172..14519
                     /gene="rbfA"
                     /locus_tag="IVW29_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7839316.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4958 a         2583 c         3237 g         3769 t
ORIGIN      
        1 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
       61 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      121 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      181 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      241 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      301 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      361 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      421 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat
      481 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      541 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      601 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      661 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      721 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      781 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg
      841 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
      901 gcaatcttag cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta
      961 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1021 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1081 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1141 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1201 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1261 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1321 acgattgaag gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta
     1381 ttgctatacc cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg
     1441 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1501 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1561 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1621 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     1681 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     1741 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     1801 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     1861 aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     1921 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     1981 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     2041 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     2101 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     2161 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     2221 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     2281 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt
     2341 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     2401 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     2461 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     2521 aggtaaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     2581 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     2641 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     2701 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     2761 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     2821 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     2881 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     2941 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     3001 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     3061 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     3121 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     3181 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     3241 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     3301 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     3361 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     3421 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     3481 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     3541 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg
     3601 caacggaaga aagtttggac gagacatata aagattacga aaaagcctat accgaagtat
     3661 tcaagcgttg tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga
     3721 aagactcaaa ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     3781 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     3841 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     3901 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     3961 cggatgaaca acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac
     4021 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     4081 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     4141 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     4201 tccactataa aaatgtgaat ccagaacgcg atttcactgt gctttcttat gaagatcttc
     4261 gttttgtaca agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag
     4321 gaatcgaaat cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     4381 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     4441 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc
     4501 caaaaggaat tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc
     4561 aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     4621 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     4681 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     4741 aaactggcga aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct
     4801 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     4861 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     4921 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     4981 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     5041 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     5101 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     5161 gaatttacgc tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     5221 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     5281 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     5341 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     5401 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     5461 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     5521 gtggatgagc agcaagcgga acgagtattg aaactttttg aagaacgcaa acaagaacag
     5581 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     5641 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc acatccagct aggacgaaat
     5701 attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga acgaagagtc
     5761 acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc
     5821 ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc
     5881 gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa ggtacgagga
     5941 agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa
     6001 gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt agagcttcat
     6061 gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct tgtggcacaa
     6121 gcagggaaat ggggacaccg agctatcgcc attacagacc acggtggtgc gcaagccttt
     6181 ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg
     6241 aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc attgagtgaa
     6301 gccacatatg tcgtatttga cgtggaaacg acaggattgt ctgcagtata tgacacgatc
     6361 atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt
     6421 atcgatccag gacatccttt gtcacgaaca acggtagatc tgactggtat cacggatgga
     6481 atggttcgag gatcaaagtc ggaagaagaa gtattgcgga tgttccttga attttcaaaa
     6541 gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa tactagctac
     6601 gcaaggtatg gtattcctga agcggccaat ccagtcattg atacgttgga attagctaga
     6661 tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa atttggtgtc
     6721 agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca cttagcatgg
     6781 atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca gctaaatgag
     6841 catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac gattttagca
     6901 aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa tgtcgaatac
     6961 ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga aaatctattg
     7021 atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa
     7081 gaggcaagaa accgagcaaa attctacgat tatattgaag ttatgccaaa agcggtctat
     7141 gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga aattatccaa
     7201 aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg taatgtacat
     7261 tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat gggcggagcg
     7321 aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga tgagatgcta
     7381 actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat
     7441 aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta tacaccgaag
     7501 attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa acaaatgtac
     7561 ggagatccgt tacctgagat cattcaaaaa cgattaaaaa aagagttgaa ttctattaat
     7621 ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa
     7681 gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc gacgatgaca
     7741 ggaatcactg aagttaatcc gttagcccct cattattatt gtccagagtg ccaatattcc
     7801 gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa acaatgtcca
     7861 aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac atttttagga
     7921 ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta tcaagcagaa
     7981 gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc cggaacgatt
     8041 ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag ggacaataat
     8101 ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg
     8161 acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga tgtctatgat
     8221 tttacgccga tccaatatcc agcagacgat caaaattcgg aatggaaaac gactcatttt
     8281 gattttcact cgattcacga taatgtattg aaattagata ttctagggca tgatgaccca
     8341 actgttattc ggatgcttca agatttatca gggatcgatc cacaaacgat ccctacggat
     8401 gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag tcaggaacaa
     8461 atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt tgttcgaggg
     8521 atgctagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc cggactatct
     8581 catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg agatgcgaca
     8641 ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca tgctggatta
     8701 gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg gaacaagatc
     8761 cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt gcccgactgg
     8821 tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc tgcagcttac
     8881 gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt gtattactgt
     8941 gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa aggaaaagat
     9001 gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat tagatgcttc cgtaaaagaa
     9061 aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg attcaaattt
     9121 ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg tgatacgttg
     9181 atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcaaaaca aatcgttgaa
     9241 gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg taaagtttca
     9301 aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc agatgaaaat
     9361 caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg taattttaag
     9421 cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg gatttttcgc
     9481 tcactctttt taatggaatt acgcacttaa ttttttaagg aggcgaaact tttgagtagc
     9541 gtcgttgaaa cagtcacaga aatggtgaca ccgattttag atgaacaaaa ttttgaactt
     9601 gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct tacgagtatt tattgacaaa
     9661 gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt
     9721 gatgcagcag atccagatcc gattcctcaa gcttatttcc tggaggtatc ctccccggga
     9781 gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg cacttggtga ttatgtccat
     9841 gtctccttat accaaccagt agatggcgaa aaacaatatg aaggatttct tcaatcattt
     9901 gatgctgaac agcttacatt gaaaatacgc ataaaaacac gagaaaaaga attgactttt
     9961 gaccgaaaaa atattgcaaa agctcgctta gctatccaat tttaatcacg gagaggaatc
    10021 agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg aaaaagggat
    10081 ctcaaaagaa atcgtgatcg atgctttaga agcagcattg gtttctgcat acaaacgtca
    10141 ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa agatccatgt
    10201 ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag tctcattgaa
    10261 agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt ttgaagtaac
    10321 accaaaagat tttgggcgga tcgctgcgca aacggctaaa caagttattt tacagcgtgt
    10381 acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa aagacatcat
    10441 gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg gtaaaattga
    10501 ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac atgatcgaat
    10561 caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg
    10621 cagccatcca gacctattgc gccgcttatt tgagcaagaa gtaccagaag tctatgatgg
    10681 aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag tggcagttcg
    10741 ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt
    10801 tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat ggaatgaaga
    10861 tccagctgta ttcattgcaa atgcattgaa tcctgcacaa gtcatggatg tgatcttcga
    10921 tgagacaaat ccaaaggcct gcacagttgt cgttccagac taccaattat cgctagcaat
    10981 cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg actaaccata aaatcgatat
    11041 caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga
    11101 agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat atgaaacaat
    11161 tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt aaggaggcaa
    11221 agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc
    11281 taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga tcgatccaac
    11341 tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag tacaacaagc
    11401 ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg agttttatca
    11461 agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat
    11521 acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc gggaaaacta
    11581 atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa attggtgttt
    11641 gtggcaaccg atgcaagcga gaatacaaga aaaaagataa aagataagag ttcatattat
    11701 gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat cggaaaacct
    11761 cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga gctaattgaa
    11821 ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa agaactcaaa
    11881 aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat
    11941 atgggtgcga ttactagcaa tgatgagaag aaattgcaac aagcgttcaa aaacccaagc
    12001 acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca gccgaacgat
    12061 caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg cagttatcaa
    12121 gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat
    12181 aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac
    12241 cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa taataataac
    12301 cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct
    12361 gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga aggtatgaac
    12421 gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa aaaattattt
    12481 atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat tgaactgtta
    12541 gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc tgatatcgat
    12601 aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc gccagtcgta
    12661 acgattatgg gacacgtaga ccatgggaaa acaactttat tggatacatt acgccattca
    12721 cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta ccagatcgat
    12781 attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt tacaagtatg
    12841 cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc agatgatggc
    12901 gtcatgccac aaacagttga agcaatcaac catgccaaag ctgcaggcgt tccaatcatc
    12961 gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc aacatgttat gcaagaatta
    13021 agtgagtatg agttaattcc tgaagcatgg ggtggagaaa cgatctttgt tgagatctca
    13081 gcgaaattcg gccaaaacat cgaagaattg ctggaaatga tcctgttagt cgcagaagta
    13141 gaagatttaa aagcagatcc aacacaacgc gcaatcggaa cagtgatcga agcacgtctt
    13201 gataaaggta aaggtcctgt gtcaacctta cttgtccaag aaggaacact tcatgttggt
    13261 gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa cgatcttggc
    13321 cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa tcactggctt gaatgatgta
    13381 cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt
    13441 gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg ccacaagccg tgtgactttg
    13501 gataacttat ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa
    13561 gcagatgtac aaggttcagc agaagcactg gctgcatcat tgaagaagat tgatgtggaa
    13621 ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc
    13681 ttagcagcag ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac accgcaggca
    13741 aaagtgcaag ctgatactga agaagtcgat attcgtcttc accgtatcat ctataaagcc
    13801 atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc ctgaatttga agaaaaaatc
    13861 accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta aagttggtac gattgcaggt
    13921 gcctatgtaa cagaaggata tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga
    13981 atcgtaatct atgaaggcca acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa
    14041 gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat tcaatgacat caaagtagat
    14101 gacgtaattg aaggatttat catggaagaa gtcaaaaatg actaaaaact gataaaagga
    14161 ggccaatcat tatggctaac taccgtgacc gccgagtcgg tcaagaaatc ttaaaagaag
    14221 tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta acgatcacag
    14281 atgtgaaagt tacgggggac ttacagcaag caacgatcta ttacagcatt ttatctgact
    14341 tagcatcaga taagaaaaca gcacaagatg gattgaataa agcatcagga ttgattcgtc
    14401 gagagttagg gcacaatcta agtatttata agacacctga attgttcttt gaacttgatg
    14461 aatcagttgt ctatggcaac catatcgatg aattgcttcg cgacttgaac aaagactaat
    14521 caaaaactcc cgcaaatcct gtgaaat
//
LOCUS       DACCVU010000068        14501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCVU010000068 DACCVU010000000
VERSION     DACCVU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.068"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(324..893)
                     /locus_tag="IVW29_001836"
     CDS             complement(324..893)
                     /locus_tag="IVW29_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7839317.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IVW29_001837"
     CDS             complement(967..2256)
                     /locus_tag="IVW29_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839318.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IVW29_001838"
     CDS             2610..3458
                     /locus_tag="IVW29_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7839319.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IVW29_001839"
     CDS             3472..4545
                     /locus_tag="IVW29_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7839320.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IVW29_001840"
     CDS             4535..6706
                     /locus_tag="IVW29_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP7839321.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="IVW29_001841"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="IVW29_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7839322.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="IVW29_001842"
     CDS             complement(9117..11303)
                     /locus_tag="IVW29_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7839323.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="IVW29_001843"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="IVW29_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7839324.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="IVW29_001844"
     CDS             complement(11525..11965)
                     /locus_tag="IVW29_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7839325.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="IVW29_001845"
     CDS             complement(12039..12512)
                     /locus_tag="IVW29_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP7839326.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12822..14288)
                     /locus_tag="IVW29_001846"
     CDS             complement(12822..14288)
                     /locus_tag="IVW29_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7839327.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4135 a         3027 c         2556 g         4783 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
    12661 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
    12721 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
    12781 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
    12841 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
    12901 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
    12961 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
    13021 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
    13081 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
    13141 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
    13201 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
    13261 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
    13321 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
    13381 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
    13441 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
    13501 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
    13561 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
    13621 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
    13681 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
    13741 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
    13801 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
    13861 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
    13921 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
    13981 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
    14041 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
    14101 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
    14161 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
    14221 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
    14281 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
    14341 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat ttaatacaca
    14401 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct
    14461 tattgaattt caagctattt tgaattttct gcaaaattaa a
//
LOCUS       DACCVU010000069        14383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCVU010000069 DACCVU010000000
VERSION     DACCVU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.069"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            296..628
                     /locus_tag="IVW29_001847"
     CDS             296..628
                     /locus_tag="IVW29_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7839328.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            924..1079
                     /locus_tag="IVW29_001848"
     CDS             924..1079
                     /locus_tag="IVW29_001848"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7839329.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1355..2695
                     /locus_tag="IVW29_001849"
                     /pseudo
     CDS             1355..2695
                     /locus_tag="IVW29_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2936..4552)
                     /locus_tag="IVW29_001850"
     CDS             complement(2936..4552)
                     /locus_tag="IVW29_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7839330.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4670..4888
                     /locus_tag="IVW29_001851"
     CDS             4670..4888
                     /locus_tag="IVW29_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839331.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5083..5544
                     /locus_tag="IVW29_001852"
     CDS             5083..5544
                     /locus_tag="IVW29_001852"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7839332.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5737..5976)
                     /locus_tag="IVW29_001853"
     CDS             complement(5737..5976)
                     /locus_tag="IVW29_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839333.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6000..7523)
                     /locus_tag="IVW29_001854"
     CDS             complement(6000..7523)
                     /locus_tag="IVW29_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7839334.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7541..7663)
                     /locus_tag="IVW29_001855"
     CDS             complement(7541..7663)
                     /locus_tag="IVW29_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7839335.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7693..8676)
                     /locus_tag="IVW29_001856"
     CDS             complement(7693..8676)
                     /locus_tag="IVW29_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7839336.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8700..8849)
                     /gene="rpmG"
                     /locus_tag="IVW29_001857"
     CDS             complement(8700..8849)
                     /gene="rpmG"
                     /locus_tag="IVW29_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7839337.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8870..9247)
                     /locus_tag="IVW29_001858"
     CDS             complement(8870..9247)
                     /locus_tag="IVW29_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839338.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9279..9458)
                     /gene="rpmF"
                     /locus_tag="IVW29_001859"
     CDS             complement(9279..9458)
                     /gene="rpmF"
                     /locus_tag="IVW29_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7839339.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9473..9742)
                     /gene="rpsN"
                     /locus_tag="IVW29_001860"
     CDS             complement(9473..9742)
                     /gene="rpsN"
                     /locus_tag="IVW29_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7839340.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10265..12037)
                     /locus_tag="IVW29_001861"
     CDS             complement(10265..12037)
                     /locus_tag="IVW29_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839341.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(11991..13745)
                     /locus_tag="IVW29_001862"
     CDS             complement(11991..13745)
                     /locus_tag="IVW29_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839342.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13855..>14383)
                     /locus_tag="IVW29_001863"
     CDS             complement(13855..>14383)
                     /locus_tag="IVW29_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7839343.1"
                     /translation="ISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYP
                     RKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWAL
                     MQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSM
                     IGVLIGRKVLKKYKK"
BASE COUNT         4591 a         2803 c         2206 g         4783 t
ORIGIN      
        1 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
       61 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
      121 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
      181 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
      241 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
      301 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
      361 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
      421 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
      481 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
      541 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
      601 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
      661 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
      721 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
      781 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
      841 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
      901 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
      961 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     1021 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     1081 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     1141 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     1201 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     1261 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     1321 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     1381 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     1441 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     1501 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa tagggctgga aaaaagtatc
     1561 catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca aacagcgctt
     1621 caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg atcgtcgctg
     1681 ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac ctctttctat
     1741 gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag
     1801 ccttcggcct aaaaccattt ctttgagcca gctgttacca gaagtggtgt gtttcgatga
     1861 attcaaatca gtgaaaaatg tctcgggagc catgagcttt gtcatgatgg acggcaagac
     1921 ccatcaactg attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt
     1981 gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg gtttcagatt tctatacacc
     2041 ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca
     2101 tattagccaa cacattggtc gggcttttac caaccaccga attcaagtaa tgaagacctt
     2161 taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac tggaaactac ttcaaaagaa
     2221 tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac
     2281 agaagcagag atagtcgatc ggctattatc ttatgatgat tccttaaagc gaggctacga
     2341 agtctaccag gattttcttt ccgttatcag aagacaagat gttcctgaat tcgtcgcgct
     2401 cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca cgaccttcaa
     2461 gaaataccaa acagaaatca aacgagcgtt gcgcgttccc tattccaacg gccctataga
     2521 atgtttgaat aaccatatca aagtactaaa acgaattgcc tatggctata gaaactttca
     2581 aaactaccga gaacgaatct ttttatatcg aggaaaatat tttaagaaaa ctaagaatac
     2641 tacccaactg actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg
     2701 tgagtatctc gattattcta ttgaatttct tcaccaacac tcattgacga agagccaaaa
     2761 taagtaatct aaccgactgt taactaaacc attgcaggtc ccgttaaaga tacttgtgcc
     2821 tgttgtcacc gcttcttttt cttgtccaca agcgcttaac acacttaata aagcccctgc
     2881 tattattaat aataatatct ttttcttatt aaaaacctcc tcatctattt ccagattagt
     2941 aaatgtagta atctgtgctt tcttttttct caatactaat aactaactta tggggtaaac
     3001 aaatactcgt ttgaccgggt tttgagatcc atccagttct aaccgcgatt tgatcaggac
     3061 tattatcttc tttaactcga attcttccat ctttaatctc aacaatatta tattgtccct
     3121 tcgcaggatg ataattcacg agtttatgat tgatcttatc caaatcgaag cgatccattt
     3181 ccttgccatc aattcgaacg atggcatatt tgatagcatc ttcagttgtt tcattctcct
     3241 tttctataga gaatacaaat aagggaataa aagaggtaat aatcaaaatg ccaataatta
     3301 tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt cattaacgat atctggctaa
     3361 acatattaga ctttttccat ggaaatggtg aaatttttcg gaaattatct gcattaagag
     3421 aatcagttaa acaaccctca agttgttcct taatacattg ttgatccatc ttcataccaa
     3481 taaaaactag tttattaatg cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg
     3541 gatttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
     3601 atgattcaat acttttatag ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
     3661 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
     3721 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
     3781 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
     3841 ccactgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
     3901 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
     3961 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
     4021 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
     4081 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
     4141 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
     4201 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
     4261 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
     4321 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
     4381 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
     4441 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
     4501 tttttcctga accaagatag ccagttaaga ttgtaatagg tatttttttc atagggaacc
     4561 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     4621 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     4681 tcgtcatctt actatactcg aacgagaaag aatctttctg cttcacgaag aaggattttc
     4741 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     4801 aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     4861 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     4921 ttatttgtcg attttcagtg gtcgccagag gaaatctcga atcgtttgaa gcatgaacat
     4981 atggactttt caattagttt tatcacgatc taacgtgcaa tttgcaatga tcttttcgat
     5041 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     5101 acaagacata caaaaaatca tgtcgagtct cgtggaaaga tttgccttac tcattcgatc
     5161 catggccgtc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     5221 gtagctggca aaactggtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     5281 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     5341 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     5401 gaattctcca atcatttcgc agttacagaa gcattgaata atatgccctt ttattttcca
     5461 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     5521 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     5581 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     5641 ttgataaagt gttgcgcttg atttgacaat tcaagacata ataaaaagca gatcaaatcc
     5701 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     5761 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     5821 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     5881 tgtactccca tttaatctca tttgctttgc aaatcgataa cgccgataag catcaatatt
     5941 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa aattgaattt
     6001 taatgagcca tcatttcttg agcaatttca tgtttagaca atgaagctgg atagaaagta
     6061 ggccaattat gcaattcatt atagagtttt tcttgacttt ctccaccaaa gaaaatatgg
     6121 aaatgctctg ccttacttgg tgcaattcca tgatcactaa attgaatata tttaaaacga
     6181 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     6241 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     6301 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     6361 taataatctt tgtactcggc agctgtcatg tctttattga tttttgcttt gtaatcgaaa
     6421 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     6481 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     6541 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttttct
     6601 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     6661 tttgtaagac tttcaagagg attcagtact tccaatttta tcccagcttc gttggccaaa
     6721 gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     6781 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     6841 ccagcaatag gaacttgttt caatccataa tccaaagcta aatatccaaa ggctgcgtgt
     6901 tgcgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     6961 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     7021 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     7081 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     7141 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     7201 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     7261 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     7321 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     7381 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     7441 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     7501 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     7561 tatttcgttt atgttctttg ataattttta atgccctttt tctagtttta atattcgggc
     7621 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccccaat
     7681 cactacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     7741 ttcatccaac atttttccga ttagaataat agtggtttgg tctttttgtc gagtggtgta
     7801 ttgaaaatta acctgttcgt ttacctcttg catggcaact ataaaatcct gctcttgtaa
     7861 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccagtcaat
     7921 ccattggagt aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     7981 ctcatgtact tgatggtgtt gatgatagta tttgccatta tagtgatttt ctgatgaatc
     8041 actttcaaaa actaagggat gattaaattt tttcaaatta taaaaatcct ttttttgaat
     8101 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     8161 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     8221 tgccatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     8281 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     8341 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     8401 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     8461 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     8521 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     8581 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     8641 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     8701 tatttcactt ctttaaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     8761 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     8821 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     8881 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     8941 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcgata cattgaaata
     9001 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     9061 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     9121 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     9181 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     9241 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     9301 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     9361 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     9421 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     9481 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     9541 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     9601 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     9661 ttaattcctg acgtttatct gcatactttt caatcatcgc catttgtttc tttgctttag
     9721 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacacctc
     9781 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     9841 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     9901 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaggctctg aaaaataaga
     9961 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    10021 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    10081 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
    10141 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
    10201 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
    10261 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
    10321 ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta ccttgatcaa
    10381 ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
    10441 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
    10501 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
    10561 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
    10621 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
    10681 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
    10741 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
    10801 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
    10861 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
    10921 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
    10981 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
    11041 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
    11101 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
    11161 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
    11221 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
    11281 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
    11341 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
    11401 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
    11461 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
    11521 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
    11581 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
    11641 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
    11701 gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac
    11761 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa
    11821 acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca
    11881 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag
    11941 aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac
    12001 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
    12061 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
    12121 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
    12181 gttttttctt ttatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
    12241 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
    12301 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
    12361 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
    12421 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
    12481 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
    12541 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
    12601 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
    12661 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
    12721 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    12781 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    12841 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    12901 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    12961 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    13021 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    13081 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattccg
    13141 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    13201 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    13261 agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa aacaggtgtt
    13321 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    13381 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    13441 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    13501 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    13561 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agactgaaga
    13621 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    13681 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    13741 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    13801 ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta cgttctattt
    13861 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    13921 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    13981 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    14041 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    14101 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    14161 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    14221 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    14281 gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg ccccaccaat
    14341 actactaacc attattgaaa aaaagtaaat tactagggaa ata
//
LOCUS       DACCVU010000070        14238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCVU010000070 DACCVU010000000
VERSION     DACCVU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.070"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            105..1148
                     /locus_tag="IVW29_001864"
     CDS             105..1148
                     /locus_tag="IVW29_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7839344.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="IVW29_001865"
     CDS             1321..1545
                     /locus_tag="IVW29_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839345.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="IVW29_001866"
     CDS             1548..1805
                     /locus_tag="IVW29_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839346.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="IVW29_001867"
     CDS             2022..2897
                     /locus_tag="IVW29_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839347.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="IVW29_001868"
     CDS             2907..3701
                     /locus_tag="IVW29_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839348.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3730..4848
                     /locus_tag="IVW29_001869"
     CDS             3730..4848
                     /locus_tag="IVW29_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839349.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4881..5201
                     /locus_tag="IVW29_001870"
     CDS             4881..5201
                     /locus_tag="IVW29_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839350.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="IVW29_001871"
     CDS             5456..6145
                     /locus_tag="IVW29_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP7839351.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6182..6349
                     /locus_tag="IVW29_001872"
     CDS             6182..6349
                     /locus_tag="IVW29_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839352.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            6369..7997
                     /locus_tag="IVW29_001873"
     CDS             6369..7997
                     /locus_tag="IVW29_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839353.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8048..8419
                     /locus_tag="IVW29_001874"
     CDS             8048..8419
                     /locus_tag="IVW29_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839354.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8504..8704
                     /locus_tag="IVW29_001875"
     CDS             8504..8704
                     /locus_tag="IVW29_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839355.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8838..10064
                     /locus_tag="IVW29_001876"
     CDS             8838..10064
                     /locus_tag="IVW29_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839356.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10914..12239
                     /locus_tag="IVW29_001877"
     CDS             10914..12239
                     /locus_tag="IVW29_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7839357.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            12232..12579
                     /locus_tag="IVW29_001878"
     CDS             12232..12579
                     /locus_tag="IVW29_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839358.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            complement(12964..14151)
                     /locus_tag="IVW29_001879"
     CDS             complement(12964..14151)
                     /locus_tag="IVW29_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7839359.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         5389 a         1902 c         2802 g         4145 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggataat catgtgaata tgaacttaac aattaaacgt atttacgatc
     7981 ttttatcact tatataaaga gagaagtgat aaataggaca caaaatttgg aaagtaggta
     8041 agtcactatg agagaactaa ctttatcttc taaggaatta gaaaagaaaa ctgctttatt
     8101 aaacgaaata tttagtgctg attgtataat ccaaagttta aaagaaagtt cattttcaaa
     8161 actaacagat caggaaaaaa tttcttatat gctaagttta gataatctag gaactaaata
     8221 tgatgaatca gcaagtagcg ataagctttt gattgaatta gtatcaagtc ttagtagttt
     8281 tggaaaaatg aaaagattta atgtgatatt agaaattttt gcttggttaa tcccttcatt
     8341 tatttggttt ttttatattt tcatagttga atctgagtta atagccaagg tctctcttgg
     8401 actgttaatt ccgatataaa taggttcagc aatttacttt aataacttgt atacacgtat
     8461 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
     8521 atgggataaa gataataaaa cagatattga gcttaaagaa tataaataca attatttctc
     8581 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
     8641 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
     8701 ctaaaagcaa ttgaggtcga aattccgata cttgaatcat gatctatatt atcgtgttat
     8761 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
     8821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaat
     8881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
     8941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
     9001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
     9061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
     9121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
     9181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
     9241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
     9301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
     9361 cgcctagaat atataaaaat agaaacaaat atggattact accttttaga ggtcgagata
     9421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
     9481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
     9541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
     9601 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
     9661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
     9721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
     9781 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
     9841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
     9901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
     9961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    10021 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaaatt aaacaatcgt
    10081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    10141 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcatg
    10201 tttctcaaat ttttaggata agggaaattg ctagcagttt gttttagaac catataaaac
    10261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    10321 taaaacaaca tggatttaaa acaagaagta agtcagttct ccttttactt tagtaaggaa
    10381 aaataaagta ttctcaataa aacttaaaat aagcaaacct ttgttttaat agctaatcga
    10441 agtaaaaccc aaagccatat gaacccctga atcagcgaaa gtttatgatg atttggatta
    10501 ctaatagttt aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa
    10561 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttatgt gaccagacgg
    10621 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct caaattgttt
    10681 aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat
    10741 gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga
    10801 acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc
    10861 tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa tgggggaata aaaatgtttg
    10921 attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta
    10981 gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta
    11041 gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat
    11101 atcatattac taatgttagt agagctactg atttacctta tccattacca aaagaacttc
    11161 atattgttcc accacgaatg aacttatata taaaaaaaaa tatggaagtt aatcagattt
    11221 ttagaaagta tgtcgcagat gaagatttat tgatttattc gattgatgaa tcaattttaa
    11281 aagtgacaaa atccttaaat ttatttacta gttcaggaac aagaagcgag agaagaagag
    11341 aattagctaa aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg
    11401 gaataggaga taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta
    11461 aagaatttat agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg
    11521 agatgactga cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa
    11581 ttatgagtat agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg
    11641 taatcggact ccagttatat ttccatacca atggaattga tcgtacggat attgctattc
    11701 caccagaacc aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata
    11761 cgaggagaga agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa
    11821 ttagacaaca caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg
    11881 aagaaaaatc aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat
    11941 tacaaaatta ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata
    12001 taggagtaac ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg
    12061 atccaagaaa aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa
    12121 aatatggatt tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta
    12181 cgagaagcac tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa
    12241 tataacgata gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga
    12301 atcaatgcag acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact
    12361 gaagagattg cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa
    12421 aaagaggaac gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga
    12481 tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat
    12541 gtttctttct cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat
    12601 tcggttatat tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa
    12661 ttaatgaagt ggggattgat tgcttttgtt tctatattga atccggagca ggataccata
    12721 agaacagata ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt
    12781 tctccaaata cttagtcaag tccacaatct tgtgtaaaag ttcaacctca tcaaaagcac
    12841 aatgttttgc gttgtgaacc taactgccat gcctttcttt ctgaggagag aggtcaggag
    12901 cagtagcgca gcgactcttg atgtctctcc tcagaaagaa gagactcgca caggttcatg
    12961 gtgctaaatg ttattcgaat taaattctaa atattttcgt ggagaagcca gccactcagt
    13021 gtctttttcc atcaaaagtg ttccaattat ccgagtgatg gaattgatat ttggaaaaat
    13081 ccgaatcact cgttctctcc ggcgaatctc ttcgttcaac cgttccaaca cattggttgt
    13141 acgaatcctt cggcggatat tttcaggaaa agacaaaatt tgaatagcat cttcaaagcc
    13201 gttttctaaa atgtcgcaag cagcacttaa ttttgaatca caaccatatt tttccaagaa
    13261 gtgctctttt ctttcacgtg tcaattcaag ttcagaggct ttaaagatac tcttcaactc
    13321 gtcttttaca tcagaagaaa ctttctttgg cagcttatca aaaatatttc tcaaaaagtg
    13381 ggcttggcat ctttgccagc tcgcacctaa aaagttcttg cgaacagcct taactaaccc
    13441 tttgtgtgca tcagaaatga ccagttttac accgaacaaa cctcgttgaa cgaacgattg
    13501 aaaaacggta tcccaattat cttccgattc gccgtcttga acatcaaaac caagaatcgt
    13561 acgtcgtcca tcagaatcaa tgccgatacc aatatataca ccttttgaga caatacgatg
    13621 attttccctt actttcatat acagggcatc acacatgaaa aatggatatt ccgagccttc
    13681 taggctccgg tttctccact caaaaacaaa tggatccaac tgtttcatca gatttgaaac
    13741 gaatgattta gaaacagtcg caccatccgt tagcagttca actgttttag ttactttgcg
    13801 agtggagaca ccagaaatga ccatttcaat catggcagcg atcaaagctt tttcgcttcg
    13861 ctgatatctt tcaaagatag taggactaaa cttaccgtct ctggttcgtg gaacatgaag
    13921 ttctaacgta ccaattcgag ttgtaaacga acgatcgtag taaccatttc tttgtgattg
    13981 ccgttcttct gaacgttcat aggcatttgc ctgaatatat tgatttcgtt gttcttccat
    14041 cagctgatta aacatgatag tcataagtgt ttttgcggta tcattcgcac cgctttcgtt
    14101 tataattgct tgaatttctt cgctttcaaa agtaaaatgt acttgagtca ttgatatcag
    14161 tcctattcga gtatgttttc tgcaaaaaac attgtactcc taactgagat caatggcttt
    14221 tttgatgctt cactttta
//
LOCUS       DACCVU010000071        14043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCVU010000071 DACCVU010000000
VERSION     DACCVU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.071"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            476..727
                     /locus_tag="IVW29_001880"
     CDS             476..727
                     /locus_tag="IVW29_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839360.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="IVW29_001881"
     CDS             761..1039
                     /locus_tag="IVW29_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839361.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="IVW29_001882"
     CDS             1636..1893
                     /locus_tag="IVW29_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839362.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="IVW29_001883"
     CDS             2006..2617
                     /locus_tag="IVW29_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839363.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="IVW29_001884"
     CDS             2796..3353
                     /locus_tag="IVW29_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839364.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="IVW29_001885"
     CDS             complement(4052..4573)
                     /locus_tag="IVW29_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7839365.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="IVW29_001886"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="IVW29_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7839366.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="IVW29_001887"
     CDS             8653..9540
                     /locus_tag="IVW29_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7839367.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="IVW29_001888"
     CDS             9537..10706
                     /locus_tag="IVW29_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839368.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="IVW29_001889"
     CDS             10730..12085
                     /locus_tag="IVW29_001889"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7839369.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IVW29_001890"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IVW29_001890"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7839370.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         4567 a         2438 c         3033 g         4005 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gac
//
LOCUS       DACCVU010000072        13959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCVU010000072 DACCVU010000000
VERSION     DACCVU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.072"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..135
                     /locus_tag="IVW29_001891"
     CDS             <1..135
                     /locus_tag="IVW29_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7839371.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVW29_001892"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVW29_001892"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7839372.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IVW29_001893"
     CDS             complement(2412..2663)
                     /locus_tag="IVW29_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7839373.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IVW29_001894"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IVW29_001894"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7839374.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="IVW29_001895"
     CDS             3613..3831
                     /locus_tag="IVW29_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839375.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="IVW29_001896"
     CDS             complement(4120..4632)
                     /locus_tag="IVW29_001896"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7839376.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IVW29_001897"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IVW29_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7839377.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7519)
                     /locus_tag="IVW29_001898"
     CDS             complement(7085..7519)
                     /locus_tag="IVW29_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7839378.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="IVW29_001899"
     CDS             complement(7529..8317)
                     /locus_tag="IVW29_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7839379.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="IVW29_001900"
     CDS             complement(8314..9255)
                     /locus_tag="IVW29_001900"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7839380.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="IVW29_001901"
     CDS             complement(9432..10019)
                     /locus_tag="IVW29_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7839381.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IVW29_001902"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IVW29_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7839382.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="IVW29_001903"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="IVW29_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7839383.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="IVW29_001904"
     CDS             complement(12936..13871)
                     /locus_tag="IVW29_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7839384.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACCVU010000073        13720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCVU010000073 DACCVU010000000
VERSION     DACCVU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.073"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            61..255
                     /locus_tag="IVW29_001905"
     CDS             61..255
                     /locus_tag="IVW29_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839385.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            524..823
                     /locus_tag="IVW29_001906"
     CDS             524..823
                     /locus_tag="IVW29_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839386.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(920..1354)
                     /locus_tag="IVW29_001907"
     CDS             complement(920..1354)
                     /locus_tag="IVW29_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839387.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1586..2296)
                     /locus_tag="IVW29_001908"
     CDS             complement(1586..2296)
                     /locus_tag="IVW29_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7839388.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2286..3146)
                     /locus_tag="IVW29_001909"
     CDS             complement(2286..3146)
                     /locus_tag="IVW29_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7839389.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3151..3789)
                     /locus_tag="IVW29_001910"
     CDS             complement(3151..3789)
                     /locus_tag="IVW29_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7839390.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3804..4103)
                     /locus_tag="IVW29_001911"
     CDS             complement(3804..4103)
                     /locus_tag="IVW29_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839391.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4146..5630)
                     /locus_tag="IVW29_001912"
     CDS             complement(4146..5630)
                     /locus_tag="IVW29_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7839392.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5650..6111)
                     /locus_tag="IVW29_001913"
     CDS             complement(5650..6111)
                     /locus_tag="IVW29_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839393.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6129..7196)
                     /gene="ulaG"
                     /locus_tag="IVW29_001914"
     CDS             complement(6129..7196)
                     /gene="ulaG"
                     /locus_tag="IVW29_001914"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7839394.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7414..8184
                     /locus_tag="IVW29_001915"
     CDS             7414..8184
                     /locus_tag="IVW29_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7839395.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8457..9284)
                     /locus_tag="IVW29_001916"
     CDS             complement(8457..9284)
                     /locus_tag="IVW29_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839396.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9852..11087
                     /locus_tag="IVW29_001917"
     CDS             9852..11087
                     /locus_tag="IVW29_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7839397.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12086..12346)
                     /locus_tag="IVW29_001918"
     CDS             complement(12086..12346)
                     /locus_tag="IVW29_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7839398.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12373..13674)
                     /locus_tag="IVW29_001919"
     CDS             complement(12373..13674)
                     /locus_tag="IVW29_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839399.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4402 a         2819 c         2167 g         4332 t
ORIGIN      
        1 tcattttcac ttacatatat ttgaatatta actaaaaaca gcaagaaaga agaggatcaa
       61 atgtattatg tagaagtaaa aacaaaaggc gtcaaaaaca aacaatatgt gaaaggtatc
      121 agtaacgaat atcctttact tggttcatgg aaagaagctg ctcctttttc caaaccatgt
      181 gccataaaaa taaagaacga gctagaaaaa gaactgactt gtggtaaagc tgttgtagat
      241 atcatcgaaa agtaaatcca atgtatagaa aggaagatca cacatgaaaa gcgacgtaca
      301 aattgctaag attatagaat tagaagaaga agacgcagtg ttgacaattg aaaaaaaata
      361 aatgtttgta taccatatga ttatttcaat atgtatcctg gaattggaga tctcgtagag
      421 ctatatcaag atgaatcaaa tatacttgtc attcatcatg aaatatagag acttataaaa
      481 ttgtttattt ttataatgta tcagtataga aaggaaataa aacatggatt atactgttga
      541 atttgacagt gaactactta agcaatttcc acaagaagaa catttcatgt ataaagaagc
      601 aatttcccac aaagtgacag agtatttgga gcataatttt tttcgtataa agccagtgcg
      661 tactcgttcg gcaaaaggaa tatacgagat gaaagtcaaa attaataaaa aaaattatcg
      721 catagcattt gctttaaaaa aaaatcatgt gttcattttt tatattagtt cgaatttgca
      781 aaaaattgta tttgataaag aggtgagtaa aaaaataaaa tagctgagca ttggaggata
      841 gattgagtga tactaagtct atcctcttgt ttttctaaag aactaagcta gaattttttc
      901 ttttgtacgt tattatatgt tatttgtcat taccaaaaag cacctttttt cttctagtat
      961 ctatgacata agaaatacca cccatttcct ttacttgaac aaatcctact gattcataaa
     1021 atttttttgt tttctcagta tcatctgtca ataattcgat ttggactggt ccataactag
     1081 tgatcttctc caaagcttca ttcaaaagca ctttgcctat tccttggcgc tgatacctcg
     1141 gatgaatgag gatatcttga ataaatgcga tcgtcgcaaa atcagtgatc caacgaacga
     1201 ttccgataat cttcccttcc ccattacgct ttatcagaga ttcagattgt tcaaatgctc
     1261 tagctaacct tgcagtgtct tttgtgtaat tcgtccaacc aacgctctta tataattcag
     1321 aaatctcatc gatcataagg gaatcaatat tcattttttt cttcctttct tatacaattc
     1381 tccctctaat aatacagttc tttgactatt taatctatca catataactc tcctgtaaag
     1441 attctctcga ttccttaaaa ttcctctttc cttttagaat gcatactttt tgataaataa
     1501 aaaggttgtg ataaattttc ttcacaacct ctgtttctct ctatcaagtg ttatctttta
     1561 attttctacc cttaccctag catctctatt gcccgtagta tgcatacttt ccatgtttgc
     1621 gaaaataatg tttatctagt aaatattgcg ggatttcaca tatattgggg ttgatcagct
     1681 cgttttcttt ggccatcaga caaatttcat ctaagatcaa actgttttct actgctttca
     1741 taggtgaatc cccccatgtg aacggtccat gtccatagac aagtactcca gggacttcgt
     1801 ttggatctaa ttttctttcc ttaaacgtct cgacaatcac attccctgta tgaacttcgt
     1861 aagcttctct tacttcttct ttcgttaatt ggcgtgtaca aggaacctta ccataaaaag
     1921 catcagcatg agtcgtgcca taagctggca aatcgcgtcc tgcttgcgcc cacatgacgg
     1981 agtgcgtgga atgagtatga gtaatagcag ttatatcttc aaaagtttga tacaaaacaa
     2041 catgtgtggg gagatctgaa gatggtttta agctatcttc ttctattata tttcccgaca
     2101 aatctgttac caccatttga tctgcttgca tgcattcata tctgacaccg cttggcttaa
     2161 tgacgatgac tcctagctca cggtccactt cactcacatt gccccaagtc aatttgacta
     2221 atccgtatcg cggcaatgcc agatttgctt caaggacacg ttgcttcatt gctgcaattt
     2281 tttcttcaca agacatcata gccagcctcc ttcaatttag aacttaagta gtcttttgcc
     2341 tgctggattt ctcttcgaaa gtcttggctt ttttcactcc acatttcaat cagaaatgtt
     2401 ccatcataat ctagacgttt caaatgtctt aataatccaa gaaaatcaac acaaccgcta
     2461 ccaaaaggaa catcacgaaa ctggcctgaa tattcttttg ttacagggta agtatctttt
     2521 aagtggatag cagtgatata atcgatccct ttctcaagtt ctcgagccgg attattttcc
     2581 ggccatgcgg acaaattgcc taggtctgga tatacttgta gaaaaggtga atgaatctgt
     2641 tctttgattt ctaaaaattt tgaaatggag ttcataaatg gatcatccat gatttccacg
     2701 gaaagaatga tgccatattt agaagcttcc tttacgcttt cttttattcc ttcgataaag
     2761 tcttctctgg aattcattga tttctcttca taataaacat cgtatccagc tatttggatt
     2821 actttgacgc ttagattatg cgccaaatgg attgcttttt tcattagttg tttcgctgct
     2881 tttttctttt ctggatcagc agaaccaaac ggaaatcgcc gatgcgcact taaacaaatc
     2941 gaatggatac gtacaccgga gctgaacatc tcttctctca gctcagctat cttttcttca
     3001 gaccaatcta atcgtgctaa ccgctcatcc gtctcatcaa tcgacatctc gataaaatcg
     3061 aagtccattt cttttacaag ggaaaaacgt tctttccatg aaatatcttt gggtaacgct
     3121 ttttcataaa tcccaatttg agccatttcc ttacccccag atacgaatga tctcatcttt
     3181 gaagttttgt gcggccttca tcggatcatt tgctgctgta ataccacgac cagtgataaa
     3241 ggtatacaca tcgatccctt caaataagtg taatgtgtgt ggatttaatc caccagttac
     3301 agaaacatta aagcctagtt caatcagttt tttgatcttc gataaatctt tttcacccca
     3361 agtttcacca gataacagcg catcacggct ttgatggtag attacctgcc ggataccgat
     3421 gttatgccaa tccatggctt gttcaaaagt ccaattgccg tagagttcta cttgcaattc
     3481 acctatttct ttttgtgctg cctccatagt tggaatcgtt gcacaacaaa tgactgtcat
     3541 ccagtctgct cctgcttttg caacattact cgccactgtt cctccagcgt ctgcacattt
     3601 cgtatcagcg actagttttt tatctgggaa catactgcga atacagcgaa tcgctttttg
     3661 tccttcctgt aagcaaagaa ttgtgcccac ttcaataata tctactacat caccaacttt
     3721 cataacatct cctaatgcat gttctaagtc attgtgatct aacgcaattt gcaaattcgg
     3781 cctgctcatc ttagttcctt cctttacttg tttttttggt accactttgt ttcagcaaat
     3841 ttttcactga tttctttgac tgacatcaca tttttgatac cgataactgt aactcctttt
     3901 ttctctgcct gagtaaacat attcaaaaaa ttgactggcg taaaaactac atcgtattgg
     3961 ccagctgaac tttttccctc tgaaatggca caatgatgga ttttggtaat tggtacgttt
     4021 agttctttca ttgctttttc cacacttctc atcatcatca agcttgttcc tgatccattt
     4081 gcacacgata ctaaaattct catcttaatc cctccattat gagtgttgtc taatcagatt
     4141 ttcttttata ctctctgctt ttctgcatat gtgtcatagt cttctacaat caaaaaataa
     4201 ccatcaggat ttttccgata ttgtaactgc ggaatagcca ataaaccaat gacaactacg
     4261 gcaattccag cataacctag gactttcatg attactgtaa acaatggcca agctgttgcc
     4321 cagtcgaaca tcccgatata accaccgtat gttgacatcc caatccatgt agcgaataat
     4381 gctgaaccgc ctacttgtat gattcctgaa agaaacggga acaggcaagc ggctttgaat
     4441 ccacctcggt tattggcata aactgctata acggcattat caaagaacaa cggaatgaaa
     4501 ccggctatga cgatcgttgg tgatttcaat aaaatcagta aaccaatcat caaaaactgt
     4561 cccaaagcac caaacaagaa cccaatcgtt accgcatttg gcgaaccaaa gccaaaagta
     4621 gcagctacgt caattcctgg aacagctcct ggtaaaatcg tgtttgagat tccttggaaa
     4681 gattgagtta gttcatcgac gaatgtccga acacctaatt gcaagatcgc cagatatacc
     4741 gcaaaattta aagaagtcgt cataatgtag aataagaaac tctgcccttc ttgcatgaat
     4801 tgcgcttgaa tcaaataatc tttgcctagt acaatcagaa tgatcccaaa gaaaaatagc
     4861 atgagtaacg aagtagcaac catattctca ttgaaaatcg acaagaatcc tggtaatttg
     4921 atatcttcaa tttttttgtc ttgcttgatt gatctatttt tcttttcatc ccgctttttc
     4981 atccactcag ctaattttgc aaatataaag attccgaaca tttgctgatg tgccactgca
     5041 aatccggcac cttctgtcaa ttcctgcgtg attcctactg ttaagttaga agctactgtc
     5101 caataacaac ctaaaatcag tcccataaaa atcaatgctt gtatcctacc tagatcagga
     5161 aagcaaaaca gtaaaatcca aaaagcggta gctgcttgtt gaacctgaac gtttccggta
     5221 gtgaaaacgg aacgcagttt cgtatatttc tgaaaacgga ctaataagat atttaaaaca
     5281 aatgcaatca gtaataaaat catcacatct ccaaaggtac ggccaaatgt ctccattaat
     5341 ccagcatcca cagcattttg tccaaaataa ggatctgtaa ccatggcatc caaattgaac
     5401 cgttccttta gtccaaccaa gattggccga aaattattga ccaaaccacc agagccaaca
     5461 gtcaaaataa agtaaccgac cgttgccttt aagaatccag cgatgcattc atgaagcggg
     5521 cgtttcagca aaatatagcc taataacacg ataaaaccaa tcaggtatgc tggctgcgtc
     5581 aagatatttg ttgcaaaata cgtccaaatg cttaacaaaa attcccccat cttttttctc
     5641 ctctatcaat taattatatt ctgccatcac aacttggtaa tcatcgatgg agttcgtaga
     5701 gatgagacgc tcaaccaatc catctgtcat taataattcg gataattttt ggatattttc
     5761 tagatgttct tctggatctt ttgctgcgag tgtaaaaaac aaactagccg ttttctcctc
     5821 attctcccct tcactttcaa aacggattgg ctgtttcaac ttggtaaatg aaatagctgt
     5881 tccaaaaaca ccatcacttt tttctgaaga atgcggcatt gctacttgag gtgcgatcac
     5941 aatataagga ccgtattttt ctacacaatg gatgatctcc tccacatacg tttcatcaat
     6001 aatttcctta tcgatcaata caagacagct ttcggcgata gcttcacgcc aatccgtgat
     6061 ttctttatct gggtaatgaa tcaattgatg gtcaaaaaag tatttcagca agaaactccc
     6121 cttctttctt ataaaaacga cggaaacggt gtatcatttt caatcgagaa gacatcttca
     6181 aatcctcgat aaaaattaaa ctcaagatca tctttgtttg tcggatagat gaatttcccg
     6241 cctacttgcc agatataagg tttaaatcca tacttcaggc gatccttttt gaatttccaa
     6301 atttcagtga tctcttttgg atcagccata aaattagacc aaatatcgta gtggacagga
     6361 atcactactt ttgcattcaa tgactccgcc atgcgcagca tatcaacaga agtaactttg
     6421 tctgttatcc ctcgtgggtt ttcaccatag gcccctaaac agacatcgat tttgtgttca
     6481 ttaccatgtt tggcaaataa attggagtaa tgtgaatctc ctgcatgata aagattccct
     6541 cctgatgttt cgaataaata gtttactgca atttgatcca tctcctgagg catttttcct
     6601 tttaatacct cttctggatc ttcacaagtg accaacgctg tccgatcaaa cgcttctaaa
     6661 gctactacat tgatgtcttt gattttgact gcatcacctg gctttactgt aatcgttttt
     6721 tcttcaggaa ttccccattt ttgccacgtg cgaaccactt cttttggccc aatgaatgtt
     6781 gcttcaggac aattttgatg aactgctgcg gctgtatgga tatctagatg atcggaatgg
     6841 atatgtgtaa cgattaaagc gtctacattt tttatcgcaa aaggatcgat tacgaaaggc
     6901 tgagtacgta aattaggctg catattttga acgccactca tacgcatcat ctgatggcct
     6961 tttttcatca tgccattccc atgactacgt tttcccgtcc cacaccatag atcacataaa
     7021 atattcgttc cttcatgact tttcagccaa attcccgtac agcccagcca ccacatagca
     7081 aaagttccag gatccactac ttctgtttcg atttcttcat tcaagtatgt gccccactca
     7141 ggaaatgttt ctactaacca cttttctttt gttacttcat tgacagtttt actcatttgc
     7201 ttgctccccc catatcgttg ttccaactta caagtaaata ttaatgtgta tcagcatcca
     7261 tgaaaaccct tttatatgac cgtttgtttt ttatatgatt ttctttcagt aatcaatgat
     7321 tgtttcagta taataaagac agatatgaat gtttaaatga acaactagtc aaataaatcc
     7381 atggatcaag atgtccctca ggaggcaaaa gtcatgaaaa actcacttgt caatattgaa
     7441 aaacgtcaaa aagaaatttt ggaactgctt cagcagactg aacaactatc aactttcgaa
     7501 ttagctgaat cccttgatgt atctattagt acgatacgaa gagatttaaa tttattagaa
     7561 gaaaaaaacg agatcatacg caattacggt tattgttctt ataattacaa aaaccagact
     7621 gattttgatc aatctggtcc tgaaaggatt aaacaagcga ttgccagaga agctagtaaa
     7681 tacattaacg attatgatac gctgtttatc aattctagct cgactgcgtt gaaaacactg
     7741 gattttcttc aatctaacca tttgacagtc attacaaaca acgtgaaaat caattactct
     7801 ccgcacaaag ataattgtag ttatatttta actggtggag agatgcgctt tccaaaagaa
     7861 gcactagtgg gcgacatcgc attaaatacg atttcttcag tcaatgcaga catttgtatc
     7921 attggctgta gcggagtcga tgtagtacat ggggtcacaa caaagatatt aaatgaatca
     7981 acgatcaacg aaatgatggt caacaagaca atcaaatgta agattttagt cgctgatcat
     8041 cgaaaaatcg gactgacatc aaagttcaag attgctgata tttcagtctt tgattatttg
     8101 atcactgata aattttgttc tgaaaaagta ttagaggaga tcagaaatac ggggataaaa
     8161 accattcaag tagattcatt ttaagaacca ggacgtaaat atgtaaatag gattatcaaa
     8221 aaaatgaata acaaatttat cgataaatga aaaacacgct acgctcaaat tgataaagga
     8281 atcccaagat gtatccatcc aatcgatacc atcataatct attgcttctt ttataaccgt
     8341 tatatgttgc ctaaatagga atcaaacaat atcgaaaaaa ggaggtatga caaaagtcac
     8401 acctcctttt ttcgaataaa cggtattcaa atcagaacac tgatttcaca accactttat
     8461 ttcaggtcgt tttcaatcac ctttaccgcg taatcatttc cgttgaaacg cgctattttt
     8521 tttgcgtcca tatatctttc taaataatca tcttctccta attgcttatg gcaagaagca
     8581 atttcataaa gcaagtattc caatgcataa gtcgatttat gtttcttatt atactcaata
     8641 cccttctcac ataattggat tgctttatca taatcctcga ttttacggta ataggtggcg
     8701 atatcataat atacaatgca gaccatcaga ttatcaaaat cagtctcact caatatttta
     8761 agtgtacgct ccacataaac tttcgcaaaa tctaattctc cttttgacaa gtagatcatt
     8821 gccacgtttg cagctgcttg taagtgatgg atatctgttt cggtcgtttg ttccaacact
     8881 tgattaaaat aaaataatgc tgcatctggt tttttcgcat aaagatgatc aagcagtcct
     8941 tgataaaata aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt
     9001 tgtaaaattt tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt
     9061 atttttttcc tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc
     9121 agctttttgc aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata
     9181 ttgctgacag ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt
     9241 ttctttcggt attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccct
     9301 ttaaaaaatt atttctcctt tatatcatac acaaaaaaaa gttctaaaca accaaaaatg
     9361 tttagaacag aataaaaatt ttaattttaa aataatattc aaattaaaca actggcgtat
     9421 ctgctccttg atttcttttt gtgttatctg aataattatc gtttacagtt ataacacata
     9481 gtgaaaattg tcatggcgag aaagtctaag aatattttta aacataacta tttctttctt
     9541 acgtgtgtat aatgacgaca gcacacctat caattaatat aattatttta tttttttatg
     9601 ttctcatgtt tatttttaat gacacaactt ctcgtattta tctggcacaa catataaatc
     9661 gatcaaaaaa ttgttgcgtg tttttttgaa aattggagtt tcaacttgat tacaaaattt
     9721 aaatttagaa gttgtgaaaa cagcgagaga cagtttaata ttcaaaatat acataggcta
     9781 ctcgaacgct gtgtttttta gtatactagt aagaaacgaa gggatagact cattccaata
     9841 aggaggattt catgaagaca attctttttg ctcctgcagt attcaattta gctgagacaa
     9901 cacgaatgat tgaggttgcc aaagctatga aagaccgtta ttactgtgta ttctttggct
     9961 tttctaaaac ttttgctcct ttgatcacag aaagtggctt tgagtatcat ttactagcgc
    10021 cgatccttac gaaaaaacaa gaagaacaaa tcataaaatt tgaccagttg cgtacactta
    10081 aaaacccttt taccaaagaa atggttgcct ctcgtatcac tagtgaacag agtctgattg
    10141 ctaagtttga gcctcgactg attgtgacag gttcgaatgt gacaattttt ttatcggccc
    10201 gcaataaaaa gattccttta gtatatgtta aaccctatgc tttgagtgaa cctttttttc
    10261 aatcagagca tgcgctgatg ccccacgagt tacgtttctt cggcccttta agcaatcccc
    10321 tttggcactg gacaaaaaag cgtatagtaa atacccgatg gatccccaat gccttcaaac
    10381 acgctgcaca aaaagaaatg cttcctttac caaaaacaag tttacaaatg atggatgctg
    10441 atctaaatct catcacaacg cctaaactat ttttggaaga agaacagctg ccggaaaatt
    10501 atcgaatcgt tggtccaatt tttgctaagc tcagtacttc cttgcctgaa gaagtgctaa
    10561 cttttataca gcgaaagcaa caagaaagaa aacgtattat ctatttcgca atgggtagtt
    10621 caggcaatcc gcgtatgtta cgcaaaattt tgttcttctt acaaaaacaa ccggatctag
    10681 cggttattgc acctattgac tatctgcttg atgacattca atctgactca gaagatcttt
    10741 taataacaga atatctgtct agtcatcttt tgcatgaatt cattgatttt tcttttatcc
    10801 acggcgggga agggaccgtc caaactgcct gtgaatccgg caaaccgttt atcggtgtgg
    10861 ggatgcaatt tgagcaaagc tgtaatattg actattgtgt aaaatacgga aacgcaatcg
    10921 ccctcacaaa accagtgact gaaagaaaac tgcagattgc tctgcgcgaa gtttcttcac
    10981 ccaagattcg tcagcaggca cgcacgctgc aagccgtttt tccaataaat ggtccacaac
    11041 aagcaattca agaaatcgaa accttcctga gcaaccaccc aatatagttt caagtagaaa
    11101 aagctttttt aatagagact catcagtaat ttcgctattc gagacccaga cagtgaaaat
    11161 acaaaaaaag taaatagtgg attgaaaagt tagagttact taaattacgg tgtctaaaaa
    11221 acacaaaatc atccttaaaa taaaagatct gacgctatcc aaatgagcat aggtagcgtc
    11281 tgccatttat tcttagtcaa attctttgac acctttctct ggttggatca tctcaacgtt
    11341 tcttcaaaag aaagaccata ataattaggc aaatacattt tcttaaaatg tgtcatatag
    11401 atctagaagc atcgctttgt agagatttgt gccgctagtc ccctcaacaa ttactaaatc
    11461 aaatgtagga tttaccactt tatctttgat aatcaacctc cctcttctgt tgagacaatg
    11521 atctcgcaat ccgatgcata ctcctatata aactgccagc aatccccaca tattgagtag
    11581 atcatgtcag aatgttctat gtcgtcagca acgatacact aaaagtcacg ttcaccatga
    11641 ctgattgcta ttcccagcac aattatttct atacacgttt catgatttga ataaacactc
    11701 acatcataca tattaccatt catacaccat actgcagccc ctactatatg gttgtaagct
    11761 cccaaatcta tatttgactt gatcactttg gtagtagatc tactaattct aaatccattg
    11821 ctgataaaat ttccattttt tcctccttct ttctgccaaa acagttaaaa caataataat
    11881 ctttatgtaa aaaagggctg tgacaaaagc cttgtcacag tcccatattc cgaataaacg
    11941 gtggtcaaaa gcttgcatct gcggtaataa gttcaaccaa acgaaaaata agccaacatt
    12001 tcacaaaaat ttaagaagca attttcgtaa atttcttctt atttcttgaa gctaaccact
    12061 tctgttacaa ccacttttag aacatttaaa gcttcgatgt gacaaaccat ctatgctgct
    12121 cgatcataaa attgatagca aaaattccac aaagtacata tgtcccccat gaaaacaaaa
    12181 cattcatatg cggtacaatg atctttggaa gaatcacgat caaaaatgat gccagtgccc
    12241 aaatccatcg attgatttga atatgatatg tagctagcat attcaatata aggtgactag
    12301 ccgttaataa aaagataaaa acaaaaaata aaatcagcca atacattgtt tcccaccttc
    12361 taaaaaccaa acttagacag tctggacttc gttttctcca cgactttgtt catctgacag
    12421 catcttttta tcataagctt ttacaaatgg ataatagata ataaatgcta tcaatgtgtt
    12481 gatcatgatc aagataacaa cacgccagtc atttcctgct gaaagataac cgccgattgg
    12541 agctggtaat gtccatgttg gcaaaacagt aaatctattg accatttcta atttgactgc
    12601 aaaataactg ataatagtca aaactaaagg aactaagttg aacgggatga acatgtaagg
    12661 attcaacatc ataggagctc caaaaattat gggctcattg atattgaaga taccaggaat
    12721 aatagaaaaa cgaccaacct ctttcaagaa ttttgattta cagaagaata taaacaaaac
    12781 agataccacg atcgttgctc ctgcaccacc aattgttacc gtccattgat aaaactgttc
    12841 aggagcgata tagggtaatt gggtggcagc tgtaccatct gccaatgcct ttgcattctc
    12901 atccaacatg atcagccaca ttggacgaac aacagcccct acaatactca tgccatggat
    12961 accaaatgac cacaacaaat ggatcaaaaa cattggtagt aaaacaccaa gcaaattatt
    13021 ccctaaaata ttggttaaag gctcgaataa cgataataag gcagcgttta catcaaagcc
    13081 cgcgacgctt cgaatcagcc aagtaattgt taaaatgact gcacctggaa tcaatgcctc
    13141 aaaactacgg gcaacagcag gtggcacttg ttctggcatt ttgatagtaa tatttcgatt
    13201 tttaaagaaa cgatatactt ccactgcaaa aatcatagat aagatccccg caaacatccc
    13261 agatgctccc aaagaactca taggtaagac ccagccaaga ggtgcctctt caggagttaa
    13321 aacgtttaca ggaacttgta acataaagaa cgttcctaaa gataatatac cacctgtcac
    13381 actatctaat ccatatgatt tagctagatc cgaacccata acaaaagttg cataaatagc
    13441 catcaatccc atcgtcatcc taaagggtaa aacaatcgtt gccgaataag gggcaataaa
    13501 ttcattccat ccaggaaacg gtaaattgat aaagaccata aaaaatgtcc caatcaaaat
    13561 caatgacaat gttgaaacaa ccccattacg aattgctaat aaatgacgct gtgtaccaat
    13621 ttttgccatt ggtgggacaa gtttctcttc gacccatttc atcagctttt ccactaaaac
    13681 cactcctttt caataaaata tgaaccattc attgcatgac
//
LOCUS       DACCVU010000074        13387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCVU010000074 DACCVU010000000
VERSION     DACCVU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.074"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            949..1422
                     /locus_tag="IVW29_001920"
     CDS             949..1422
                     /locus_tag="IVW29_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7839400.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IVW29_001921"
     CDS             1431..1658
                     /locus_tag="IVW29_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839401.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IVW29_001922"
     CDS             2294..2665
                     /locus_tag="IVW29_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839402.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IVW29_001923"
     CDS             complement(2921..3163)
                     /locus_tag="IVW29_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7839403.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IVW29_001924"
     CDS             complement(3195..4097)
                     /locus_tag="IVW29_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7839404.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IVW29_001925"
     CDS             complement(4110..4298)
                     /locus_tag="IVW29_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7839405.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVW29_001926"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVW29_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7839406.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IVW29_001927"
     CDS             complement(4913..5806)
                     /locus_tag="IVW29_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7839407.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IVW29_001928"
     CDS             complement(5884..6822)
                     /locus_tag="IVW29_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7839408.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IVW29_001929"
     CDS             complement(6856..7206)
                     /locus_tag="IVW29_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7839409.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IVW29_001930"
     CDS             complement(7239..8141)
                     /locus_tag="IVW29_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7839410.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IVW29_001931"
     CDS             complement(8134..8991)
                     /locus_tag="IVW29_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7839411.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IVW29_001932"
     CDS             9325..10134
                     /locus_tag="IVW29_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7839412.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IVW29_001933"
     CDS             10174..10671
                     /locus_tag="IVW29_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7839413.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IVW29_001934"
     tRNA            complement(11056..11129)
                     /locus_tag="IVW29_001934"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IVW29_001935"
     CDS             11316..11639
                     /locus_tag="IVW29_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839414.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IVW29_001936"
     CDS             11803..12057
                     /locus_tag="IVW29_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839415.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IVW29_001937"
     CDS             12127..12372
                     /locus_tag="IVW29_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839416.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IVW29_001938"
     CDS             12448..12813
                     /locus_tag="IVW29_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839417.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3899 a         2474 c         2244 g         4770 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaaaa atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtg gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaa
//
LOCUS       DACCVU010000075        13382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCVU010000075 DACCVU010000000
VERSION     DACCVU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.075"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..584)
                     /locus_tag="IVW29_001939"
     CDS             complement(42..584)
                     /locus_tag="IVW29_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7839418.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            719..1108
                     /locus_tag="IVW29_001940"
     CDS             719..1108
                     /locus_tag="IVW29_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839419.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1112..1876
                     /locus_tag="IVW29_001941"
     CDS             1112..1876
                     /locus_tag="IVW29_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7839420.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2005..2952
                     /locus_tag="IVW29_001942"
     CDS             2005..2952
                     /locus_tag="IVW29_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7839421.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3082..3822
                     /locus_tag="IVW29_001943"
     CDS             3082..3822
                     /locus_tag="IVW29_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839422.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4113..4781)
                     /locus_tag="IVW29_001944"
     CDS             complement(4113..4781)
                     /locus_tag="IVW29_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7839423.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4915..5202
                     /locus_tag="IVW29_001945"
     CDS             4915..5202
                     /locus_tag="IVW29_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839424.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5618..7156
                     /locus_tag="IVW29_001946"
     CDS             5618..7156
                     /locus_tag="IVW29_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7839425.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7153..8118
                     /locus_tag="IVW29_001947"
     CDS             7153..8118
                     /locus_tag="IVW29_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839426.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8105..9427
                     /locus_tag="IVW29_001948"
     CDS             8105..9427
                     /locus_tag="IVW29_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7839427.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9490..9594
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9668..10411
                     /locus_tag="IVW29_001949"
     CDS             9668..10411
                     /locus_tag="IVW29_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839428.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10426..11418
                     /locus_tag="IVW29_001950"
     CDS             10426..11418
                     /locus_tag="IVW29_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7839429.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11430..12167
                     /locus_tag="IVW29_001951"
     CDS             11430..12167
                     /locus_tag="IVW29_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839430.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12157..12879
                     /locus_tag="IVW29_001952"
     CDS             12157..12879
                     /locus_tag="IVW29_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7839431.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12963..>13382)
                     /locus_tag="IVW29_001953"
     CDS             complement(12963..>13382)
                     /locus_tag="IVW29_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7839432.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4405 a         2291 c         2785 g         3901 t
ORIGIN      
        1 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
       61 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
      121 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
      181 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
      241 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
      301 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
      361 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
      421 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
      481 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
      541 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
      601 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
      661 tacaaaaaaa tgataaaatg attataatag aattataaag ataggttagg tgattaaaat
      721 gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata
      781 cttaggtgtc tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc
      841 gctgacagat ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat
      901 tttcggtagc gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt
      961 aggagtggtg ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat
     1021 attatttggt acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc
     1081 aggaaattta atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt
     1141 ccatcatatt ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt
     1201 tgtcatctat tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa
     1261 aattgcgcag cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa
     1321 acagccggtt tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt
     1381 tgaatttgac acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc
     1441 agttggcggg gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca
     1501 tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat
     1561 cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc
     1621 atgggttaag agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt
     1681 cttttggcat ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga
     1741 aaaaaatcgg cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg
     1801 acatatggta caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat
     1861 aaaaaagtat aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt
     1921 ttattttggg ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga
     1981 caacatgtta gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat
     2041 gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg
     2101 aaagagttag ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa
     2161 gcacttgtca cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca
     2221 actgagccct attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat
     2281 tattctctcc aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc
     2341 atcacaggtg tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc
     2401 ttgtttgggg aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca
     2461 gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc
     2521 gaagtggacg agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa
     2581 gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat
     2641 atgacagaaa actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg
     2701 ctagcactag gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt
     2761 ggtgtgattg gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg
     2821 atcaagcaag atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata
     2881 gaagaaaaag gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag
     2941 acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa
     3001 aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca
     3061 tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg
     3121 tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga
     3181 caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat
     3241 ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag
     3301 tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc
     3361 cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt
     3421 tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt
     3481 gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc
     3541 ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg
     3601 gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt
     3661 gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa
     3721 gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat
     3781 gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata caaatttata
     3841 ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa
     3901 gtttatatcg tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa
     3961 gaaaataatg gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
     4021 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
     4081 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
     4141 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
     4201 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
     4261 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
     4321 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
     4381 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
     4441 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
     4501 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
     4561 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
     4621 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
     4681 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
     4741 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
     4801 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
     4861 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
     4921 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
     4981 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     5041 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     5101 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     5161 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     5221 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     5281 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     5341 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     5401 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     5461 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     5521 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     5581 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     5641 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     5701 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     5761 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     5821 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     5881 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     5941 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     6001 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     6061 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     6121 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     6181 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     6241 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     6301 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     6361 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     6421 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     6481 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     6541 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     6601 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     6661 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     6721 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     6781 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     6841 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     6901 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     6961 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     7021 taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt
     7081 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     7141 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     7201 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     7261 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     7321 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     7381 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     7441 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     7501 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     7561 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     7621 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     7681 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     7741 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     7801 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     7861 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     7921 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     7981 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     8041 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     8101 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     8161 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     8221 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     8281 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     8341 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     8401 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     8461 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     8521 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     8581 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     8641 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     8701 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     8761 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     8821 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     8881 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     8941 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     9001 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     9061 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     9121 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     9181 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     9241 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     9301 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     9361 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     9421 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     9481 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     9541 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     9601 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     9661 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     9721 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     9781 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     9841 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     9901 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     9961 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
    10021 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
    10081 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
    10141 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
    10201 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
    10261 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
    10321 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
    10381 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
    10441 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
    10501 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
    10561 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
    10621 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
    10681 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
    10741 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
    10801 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
    10861 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
    10921 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
    10981 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
    11041 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
    11101 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
    11161 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
    11221 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
    11281 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
    11341 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
    11401 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
    11461 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
    11521 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
    11581 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
    11641 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
    11701 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
    11761 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
    11821 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
    11881 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
    11941 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
    12001 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
    12061 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
    12121 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
    12181 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
    12241 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
    12301 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
    12361 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
    12421 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
    12481 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
    12541 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
    12601 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
    12661 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
    12721 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
    12781 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
    12841 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
    12901 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
    12961 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
    13021 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
    13081 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
    13141 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
    13201 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
    13261 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
    13321 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    13381 ta
//
LOCUS       DACCVU010000076        13333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCVU010000076 DACCVU010000000
VERSION     DACCVU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.076"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            7..174
                     /locus_tag="IVW29_001954"
     CDS             7..174
                     /locus_tag="IVW29_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7839433.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(236..2047)
                     /locus_tag="IVW29_001955"
     CDS             complement(236..2047)
                     /locus_tag="IVW29_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7839434.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2044..2409)
                     /locus_tag="IVW29_001956"
     CDS             complement(2044..2409)
                     /locus_tag="IVW29_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7839435.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2572..2967)
                     /locus_tag="IVW29_001957"
     CDS             complement(2572..2967)
                     /locus_tag="IVW29_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7839436.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2985..3947)
                     /locus_tag="IVW29_001958"
     CDS             complement(2985..3947)
                     /locus_tag="IVW29_001958"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7839437.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3947..4159)
                     /locus_tag="IVW29_001959"
     CDS             complement(3947..4159)
                     /locus_tag="IVW29_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7839438.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4180..4878)
                     /locus_tag="IVW29_001960"
     CDS             complement(4180..4878)
                     /locus_tag="IVW29_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7839439.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4898..5440)
                     /locus_tag="IVW29_001961"
     CDS             complement(4898..5440)
                     /locus_tag="IVW29_001961"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7839440.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5572..6576)
                     /locus_tag="IVW29_001962"
     CDS             complement(5572..6576)
                     /locus_tag="IVW29_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7839441.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6573..7562)
                     /locus_tag="IVW29_001963"
     CDS             complement(6573..7562)
                     /locus_tag="IVW29_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7839442.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7559..8365)
                     /locus_tag="IVW29_001964"
     CDS             complement(7559..8365)
                     /locus_tag="IVW29_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839443.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8531..9487
                     /locus_tag="IVW29_001965"
     CDS             8531..9487
                     /locus_tag="IVW29_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7839444.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9564..10082)
                     /locus_tag="IVW29_001966"
     CDS             complement(9564..10082)
                     /locus_tag="IVW29_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7839445.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="IVW29_001967"
     CDS             complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="IVW29_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7839446.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10547..10993
                     /locus_tag="IVW29_001968"
     CDS             10547..10993
                     /locus_tag="IVW29_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7839447.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="IVW29_001969"
     CDS             complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="IVW29_001969"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7839448.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4150 a         2828 c         2272 g         4083 t
ORIGIN      
        1 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
       61 gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
      121 gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta
      181 tttaaaaata cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt
      241 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
      301 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
      361 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
      421 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
      481 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
      541 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
      601 ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta
      661 tcagtgctgc taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta
      721 gatgagtaaa ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt
      781 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
      841 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag
      901 cttctgaaaa gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc
      961 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
     1021 catgctcaaa atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat
     1081 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
     1141 actgatgcca ttgctccagg ttttcatttg ttagtaaccg tggccatgcc attgctccgc
     1201 cttgcttttg atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
     1261 caatcggttc cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc
     1321 ctcctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
     1381 aaaagacatt ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa
     1441 gctgatacag taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct
     1501 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
     1561 ctgtctgtaa aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa
     1621 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
     1681 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
     1741 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
     1801 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
     1861 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
     1921 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
     1981 tattatggag aatcctttct gctacactta atcctctagg tccggcacca atgatcccta
     2041 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
     2101 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
     2161 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
     2221 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
     2281 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
     2341 tctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
     2401 tttgatcata gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata
     2461 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
     2521 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
     2581 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
     2641 ataacctgct ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta
     2701 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
     2761 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
     2821 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
     2881 tttggccatt actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa
     2941 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
     3001 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
     3061 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
     3121 aaaaattctc ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta
     3181 ttccccaagt ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
     3241 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg
     3301 ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct
     3361 aggcgtacca cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta
     3421 cattcgaacc cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg
     3481 ccgtgaatca aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg
     3541 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
     3601 ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
     3661 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
     3721 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
     3781 ttcaaacgat gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
     3841 atattggtat cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat
     3901 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
     3961 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
     4021 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
     4081 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
     4141 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
     4201 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
     4261 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
     4321 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
     4381 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
     4441 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
     4501 acttgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
     4561 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
     4621 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
     4681 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
     4741 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
     4801 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
     4861 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
     4921 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
     4981 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
     5041 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
     5101 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
     5161 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
     5221 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
     5281 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
     5341 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
     5401 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
     5461 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
     5521 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt
     5581 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
     5641 aatcaaaatt cctgtaggta cttccccact tagtagaatc actctagcaa ccatatctgc
     5701 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
     5761 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
     5821 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
     5881 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct
     5941 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
     6001 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
     6061 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
     6121 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
     6181 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
     6241 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
     6301 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
     6361 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
     6421 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
     6481 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
     6541 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
     6601 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
     6661 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
     6721 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
     6781 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
     6841 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
     6901 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
     6961 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
     7021 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
     7081 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
     7141 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
     7201 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
     7261 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact
     7321 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
     7381 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
     7441 aacgcctgcc aaaaatctgc ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa
     7501 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
     7561 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
     7621 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
     7681 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
     7741 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
     7801 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
     7861 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
     7921 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
     7981 gacctgtagc atccaacgcc cactcgattg cttcatgatc ttgcgtgtcc atgccactga
     8041 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
     8101 ggtgttcact agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg
     8161 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
     8221 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
     8281 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
     8341 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
     8401 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
     8461 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
     8521 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
     8581 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
     8641 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
     8701 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
     8761 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
     8821 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
     8881 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
     8941 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
     9001 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa agatcatttc
     9061 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
     9121 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
     9181 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
     9241 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
     9301 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
     9361 ctggaagaat cttaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
     9421 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
     9481 aaattgattt taactaaaaa aagcatcttc acgattagtc gtgatatgct ttttttctta
     9541 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
     9601 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
     9661 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
     9721 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
     9781 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
     9841 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggctgcgt tgtcaacacg
     9901 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
     9961 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    10021 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    10081 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    10141 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    10201 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    10261 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    10321 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    10381 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    10441 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    10501 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    10561 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    10621 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    10681 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    10741 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    10801 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    10861 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    10921 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    10981 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    11041 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    11101 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    11161 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    11221 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    11281 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    11341 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaac aatgctttga cttgttcgta
    11401 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    11461 cgaccatttt tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    11521 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    11581 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    11641 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    11701 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    11761 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    11821 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga aatttttcat
    11881 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    11941 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    12001 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    12061 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    12121 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    12181 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    12241 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    12301 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    12361 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    12421 tgcatcccaa tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc
    12481 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    12541 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    12601 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    12661 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    12721 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    12781 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    12841 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    12901 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    12961 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    13021 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    13081 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    13141 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    13201 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    13261 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    13321 aaaccagcaa aaa
//
LOCUS       DACCVU010000077        13296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCVU010000077 DACCVU010000000
VERSION     DACCVU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.077"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(233..2308)
                     /locus_tag="IVW29_001970"
     CDS             complement(233..2308)
                     /locus_tag="IVW29_001970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7839449.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2310..3227)
                     /gene="glyQ"
                     /locus_tag="IVW29_001971"
     CDS             complement(2310..3227)
                     /gene="glyQ"
                     /locus_tag="IVW29_001971"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7839450.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3613..4431)
                     /gene="recO"
                     /locus_tag="IVW29_001972"
     CDS             complement(3613..4431)
                     /gene="recO"
                     /locus_tag="IVW29_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7839451.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4574..5473)
                     /locus_tag="IVW29_001973"
     CDS             complement(4574..5473)
                     /locus_tag="IVW29_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7839452.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5488..5889)
                     /locus_tag="IVW29_001974"
     CDS             complement(5488..5889)
                     /locus_tag="IVW29_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7839453.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5867..6343)
                     /gene="ybeY"
                     /locus_tag="IVW29_001975"
     CDS             complement(5867..6343)
                     /gene="ybeY"
                     /locus_tag="IVW29_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7839454.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6372..8564)
                     /locus_tag="IVW29_001976"
     CDS             complement(6372..8564)
                     /locus_tag="IVW29_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7839455.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8588..9559)
                     /locus_tag="IVW29_001977"
     CDS             complement(8588..9559)
                     /locus_tag="IVW29_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7839456.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10264..11643
                     /gene="brnQ"
                     /locus_tag="IVW29_001978"
     CDS             10264..11643
                     /gene="brnQ"
                     /locus_tag="IVW29_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7839457.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11925..13265
                     /locus_tag="IVW29_001979"
     CDS             11925..13265
                     /locus_tag="IVW29_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7839458.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3690 a         2836 c         2443 g         4327 t
ORIGIN      
        1 tacagttttt ttgactaggg aaattcctaa tttaaaagta aataaaacag taacaagatt
       61 ataattaatc gttttcattt ttatttcctc acttttcatg agcgttttga aaattcgaac
      121 aaagaataaa ataacaaaaa ttattaaaat agaattaata acagctattt ataacaaaaa
      181 aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg
      241 tgatgatttg atccaagctt gctaatgaca aagccatggc agctatttga cttagttgtg
      301 ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat
      361 tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag
      421 caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga
      481 acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga
      541 cacgtgtcaa agcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca
      601 attgtgctgc tgcaaatact tttgtcagat ctttttgttc cgcattcaaa acagcatcga
      661 tgacatcatg acgaatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      721 cgatcacttg ttgttgccct tctgataaac caatgccgaa acgatccgta tctttactga
      781 taacatcgct aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      841 tgatccgaac gacaccgtat gcttggcgac gtaaggcata aggatcgttt gacccgcttg
      901 gaatcatccc aactgcaaag aaagaaaaga tgctatctaa tttatctgca atcgctaaaa
      961 cagaacctac tgtagactga ggtagctctc cttcactgga tataggcatg taatgctcac
     1021 gaatcgctgt cgctacagca ggtttctcgc ctttcagaag agcatatttt tcgcccatga
     1081 tcccttgtag ttcaggaaat tcccctacca tgttggttac tagatcaaat ttatagattt
     1141 cagcagctcg ttgcaaatcc atcaattctt cctcagatag tcctacttgt tcgccaatga
     1201 tacgggcaat gacttgtacc cgttgcattt tctcatagat actaccgatt ttttcatgga
     1261 aagtaacagt ttttagtttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1321 aaaagaattc cgcatcttct aaacgagccg tcaatacttt ttgattgcct cgaaccacat
     1381 cttccaattt tacccgatca ccattacgag cagaaataaa atgaggaagc agcatacctt
     1441 gatcattccg tacctcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1501 caggaacaga aagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1561 aattgttgac ttcttctagt aaatcttcgt ctagatcaat aacccagtga tttttttctg
     1621 ctaattcgtt tgcttgtgta acgatcattt cttgtctttt ttgcggagac gcaatgacga
     1681 attgttcatt caatttttct tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta
     1741 agaaacgatg acctcgagag gctctgcctg ttttgacatc caagatttcg aatgggatta
     1801 cttcgtcatc taacaatgcc acgatccagt gaatagggcg gatatattca aaatcgtatt
     1861 ctgcccaatg catcgttaca gggaaagtca aacttttgat cacccctgct aagcctgtca
     1921 atacttcttt tgcttcctta ccatgagtgt atttcgttac atagacatat tccacacctt
     1981 ttaattctct aaaagtgata gcgtctgtcg tcagaccttg tccgcggaca aatccttccg
     2041 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     2101 cttcttcact gtccgcttgt cgttctggaa tatttgttac tttgattgct aaacggcgcg
     2161 gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     2221 ttttttcttc taattgtttc atgcttggtg tgacaacatg ggcaggcatt tcttccagcc
     2281 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacggt ttctggattt
     2341 gctgtgtttt tattcaacaa agggaatcct aatttttcgc gttctgccac aaagattttg
     2401 gctacagcac gagccatgtt gcggatgcga gaaagataac cggcacgctc tgttacggat
     2461 actgcaccgc gggcgtctaa taagttgaat gtatggctac atttcaagat ataatcatac
     2521 gccggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaag
     2581 ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat atttggaatg ttcatattct
     2641 ggttgtttga agatctcgcc atatttcacg ccatcagccc attctaaatc atagacactt
     2701 tcaacttctt ggatataaga agcaagacgt tctaatccat aagtgatctc agaagttact
     2761 ggtttacaag gcagtccacc aacttgctgg aagtacgtga attgtgtgat ttccattccg
     2821 tcaagccata cttcccaacc cacaccggca cagcccatgg atgggttctc ccagttgtct
     2881 tcaacaaaac ggatgtcgtg ttctaatgga tcgattccta aaaggcgcaa gctttctaaa
     2941 taaagctctt gaatattctc aggtgaaggt ttcatcacca cttggaattg atgatgctga
     3001 tacaaacggt ttgggttttc accataacgt ccatcagctg gtcggcgaga tggctctacg
     3061 taagcagcat tccatggttc tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta
     3121 cctgctcctt tttctgtgtc ataagcttgc atcagcatgc agccgtttga tgaccaaaat
     3181 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3241 ttcaatttta aagcgttgct gtcaaataag aatacaggaa agaaaacgac aaaaaatccc
     3301 tatgtctgat tacagacata gggacgaata attcgcggtt ccaccctact tccgaataaa
     3361 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3421 ctcccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3481 attcattttg cagttttctt gtttctataa tagatcctat cactgaacga tctatttaat
     3541 ctatggttaa taatagacgg aactccctgt gtttgtcaaa tggttttgat tttttgttgt
     3601 atgttttatt tactattctt ctttttcagg tgtctctttt tctttttcag acatgtcttt
     3661 ctctgacgtc tttaatacgt gtgcccaaga cttcatttga ttgatgaact ttttgctttt
     3721 caaatggata ccgacatatt catcatacag ctggtcgatg agttcacgga ttgcttgttt
     3781 ggtttcttct ttcagatgga ttccactaat tttatcataa gaaatagctg aaaacatccg
     3841 aacgaaataa acagctcgag gatctgcatg ataacggtga tcgtccatcg accaatgtct
     3901 ttcgcatatc acaccaccat atttggaaga atagtcaaat tttccttttg tttccccgca
     3961 tacgacgcag tgtgtccaga ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa
     4021 aatattcgtg atgatttctg catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt
     4081 aaactggaac aaatgcgggt cgtactgatg atcttcgatt gccgcatcta ctagattcaa
     4141 taaatatgtg ccatagccgc tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac
     4201 atctttgaca ctgcttaaaa atgacaaacc ttcacttcga aaattcccga tataaactgc
     4261 ttcagtaaaa gggagcaaag ccggagctaa gggctgattt tgccgatgga tctctttgat
     4321 ataaaacatc attttgccag aagattctgt aaagattttg actaatttat ctttttcttt
     4381 gtaattacgg ctagataaaa tcaagccttt tgattctgca agctggctca tattctccct
     4441 cctaaaaaat aaatctggaa aagcagattc tgccgatcca gatcatttat gttgcgatct
     4501 tccgcagctt ttctcaacac gcggattttt attcagttta tttctatacc taatttacaa
     4561 gaagctcaga atatcagtat tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt
     4621 tgtctcgcca gtctttttga actttgaccc aaagttctag aaagactttg tctcctaaaa
     4681 gattttcgat atcttttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc
     4741 cgataatgat ccctttttgg ctatctcttt cgacaataat cgtagcctgg atatggattt
     4801 tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt
     4861 ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgatgaag tattcaggat
     4921 gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata
     4981 catccatcaa acgttcgacg ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa
     5041 aaggcatctg cgttgaataa tcttcgatga ttcccaacaa ctcgtctgga tggattttgt
     5101 cgattttatt gataatcaga taaacaggag aatgcacgtt ttttagacgt tcaataataa
     5161 aatcgtctcc ccgaccgcgt ttttgatcag cactaatcat aaataagacc gcgtccactt
     5221 ctttcaacgc actgtaagct gcttctacca taaaatctcc taaacgatgc tttggtttat
     5281 ggattcctgg tgtatcaata aaaatcaact gagcttctgg tgtagtgtat actccttgga
     5341 ttttatttct agttgtctgt gccttgtcac tcatgatggc aattttttga cccacgattc
     5401 gattcaataa tgtggatttc cctacatttg ggcggccgac gattgcgaca aaccctgatt
     5461 tatgttctgg catatatgtt ctccttttta attaaacaac aaatgatata ttttagggac
     5521 aaataaaatc aacccaacga tgacggtaaa acaagcggtc actaaaactg ctccagctgc
     5581 catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac
     5641 attttcaaaa acagtattga tgatctctac tatccatacg agaaaaacag ctaaaagcag
     5701 ccataaccat tctatccgat caagttggaa aatcacgccc ataaacaaag cgaggattcc
     5761 gaacgcaaca tgtttcctca tgttgcgttc ttcctcaaaa acagttttga ctccttggac
     5821 ggcaaattcc acggacatca ggaaatgttt gtttttctct acgtttttat cttttaagtc
     5881 cataggcatc taagatctct ttctgtaaac caaacatttc tttctcatcc tctggtgtca
     5941 tatggtcata cccgttgata tgcaagaatc catgaaccgc taaaaagcca agttcccgat
     6001 catacgaatg gccgtaagct tctgcttgct ctgcagcacg ttcagtagag atcatgatgt
     6061 ctcccagtac acgaggcaat ggattctctc catcttcaaa aatcactggg atttcgtctt
     6121 cgccctcttc ttccaaggca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat
     6181 cacgattgat tacttggatt gctgcattgt ccatgaaagt aacagacatt tctgtttctt
     6241 ctggcaactc taaatatgat gctgcaaatt gcagcaagtc gtcgatttct ttcattttct
     6301 cttctgagac ttggtttgtt tcatccatga aggtaatatc cattatacat cgcttctttc
     6361 tattcttctt gctattcttc ttgttcttct tttatcttct caacttccga tttcatttta
     6421 ggatatttga tccgagaatg gaaggtactg cttaaaccgc tgatcaatga tttttctact
     6481 ttacggattt cttttaacgt caatccactg tcgtccaatt gtccatcgga aatacgctct
     6541 tcaataagat tatggacaaa actttcgatt ttttcgctcg ttggatggtc cattgcccgt
     6601 actgccgctt cacaactgtc agctatattg acgattcctg cttcacggct ttgaggtttt
     6661 ggtccaggat aacggaattg ctcttccgta acttccgggt tgcgttcctt tgccttaacg
     6721 tagaaataac gcatcagcgt ggttccatga tgctgtctac aaatatcgat caccatttgc
     6781 ggcatattat aatcttccaa tattttggca ccatctgtca catgtccaaa gataatatgt
     6841 ttactgtctt ctggcaataa gaaattgtgc ggattttccg ctcctgaagg caagttctca
     6901 acgaaaaagc tggcgtgttt gatcttgccg atatcatgat aatagcaagc tactcgagtt
     6961 agtaaagaac gtccaccgat ttctgctaca gcattagcac ttagattggc taccatcatg
     7021 ctatggtgat acgtacctgg tgcttcttcc agcaactgtt tcaataaagg atgattggga
     7081 ttgctgagtt catttaatac gatcacacta tcatcattga ccatcaattc aatatagggg
     7141 tgcagaccta tcgtcagcaa gaatgaaaac agtgcacctg tgaatccgca gactagcgtt
     7201 aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga taatcaaaat gacatccatc
     7261 atcacaggaa aaataatgac ccacatagca gcggataacc cttgttctga gatccgtttg
     7321 cgtctgacga tcgttgccat gagaccagaa aatacatagg acatcaaaat aactgtcaga
     7381 aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca
     7441 gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggagc aaaagccgcg
     7501 gggtaaaaca gcggaatata agcagcttgt tctgtttgga agagttggaa aaatttcatt
     7561 agtacgacgc ttactgacat tgccgtcacg tagaaaagta cgtattttgt tctttctcct
     7621 gcattttgga attgaagtga attataaatc agtacaccga tttgaagcaa aacagctaaa
     7681 accatggcaa tgagtgggaa aatagaagtc gtttgattcg tcaatcctag taattccaat
     7741 tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa caataatttc tccttggaag
     7801 atcatcactg gttgtacagc ttctcgtgca gattgtttta attcttctgt ctttttctca
     7861 ttcaatacat cattaactac gatgccttga tccactaagt agcgaatcat ttgttgctgc
     7921 tctgttgtga cattcaaata ttgaatctga tcttctgctt cttgtttaaa agcttccaat
     7981 tcgctttcac gaacttgttt actcatctgt tcgtcaatga gcttcaaact ttcatcccta
     8041 actgtgtgaa tttccgtttc agtcatttca aatatcgtcg aataaaaatt gttcggcagt
     8101 ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa aatttttctt cagtgtagca
     8161 atccgctctt ctgtagtagg tttcgggata ttttcatctt ccttcgcctt atctaccttg
     8221 ccattataag atttgttgat ggtatcattc gtcttatcga tcaattcgaa taattgcttg
     8281 atccgattgt tctggtcatc tgccagatct ttttgataag tatattctgg agtcactgct
     8341 tctgctgcca gttttcttct ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca
     8401 cggatactct cttctgctac ttggccttct ctgaaatcaa tggccttttg tttgacacta
     8461 ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca gaaggaaagg caatgctgcc
     8521 tttccgagtt tttcttgtag tttctgcagt gttttattca acatgtgctt atcctcctaa
     8581 gcaaagctta ttcttgatga agatccgcct tttcataagc tttgatgatt tcggcaacta
     8641 cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttt ttgatttgct
     8701 ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc acgaggcaag tctatctgac
     8761 ttgtatcccc attgacaatc atttttgatt gataaccgag acgggtcaag aacattttca
     8821 tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc
     8881 gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg
     8941 tatgatccaa tccaaaaatt tggtataaag catcataaac aggccgcaga taaggatcca
     9001 ctttttcttt cagatccccg ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc
     9061 gcgtcaaaat gattttttgc acttcgcctt tttttagggc agcaattgct aatactacgg
     9121 caagaaacgt tttccctgtt ccggctggcc ctacaccaaa tacgatgtca tgtttggcta
     9181 ccgattcaac gtatttcttt tgaccgctgt tttttacccg tataggtttt ccattacggt
     9241 ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtacct ttttgcccca
     9301 ttttcaatgc cgtcacaaca tctggcgtac ttacgtggat tcctcgtttg atcaattcct
     9361 gtaatgtccg aatgactgag gcagccaagg aaatctggga ttgctcacca atgatctgaa
     9421 tgatttctcc acgtgtatgg atggttgttt ccgttgtttc ctcgatcagt tttatatgct
     9481 tgtcgtgtgc acctaaaagc atgctgacat catcagcagc tgtcaatcta atctctaaag
     9541 aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc tttcctattt
     9601 taccatactc agaagaaact atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac
     9661 catacgtacc aagaaacgtt aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga
     9721 taaaaagaag ctgagaatgt aagttatatg aaagtggaga acaaaatatt tcgtttcatt
     9781 caacaagtat tctctaggta tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat
     9841 attcttgcat ttcaaaaaaa acaggtgtat tattacgtca acaaataaac atacagcaaa
     9901 gcgtcgagga aaacagtagg ctattcaacg atttattccc agtgagtcat gtaagtgaga
     9961 aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa acgtagatca
    10021 ttgtggctgc ctccattatc aggctagcgg ataactttat caaaaagcgt ccgtgaatga
    10081 agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc cttttcccaa
    10141 gtggaaaagg gctttttttt attttaaggt ggtggttttc atggaatctg atattagtgg
    10201 cattactaat tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa
    10261 tttatggaag aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc
    10321 ttcgggatgt tcttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct
    10381 ggcgcaaact gggtcagtgc tggcgtagga tttgctatct caggtgctct tttcccatta
    10441 ctagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
    10501 gtcggaaaat ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt
    10561 ttcggtacac cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct
    10621 gaaaaatata attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
    10681 ttccttactg tccatccaac acggttggtc aaatacgtcg gaaagtatct aaactccttg
    10741 tttcttgcat tattagcaat catttttgtc gtttctttat tccatccaat gggaaatcta
    10801 aatcaatctg ctgcagatac ttatcaaaca aacgctttaa caaatggtgt cctagaaggt
    10861 tataacacgg ttgatgcagt agctttgctt gccttaagtg tcacttttgt ccatgcagtc
    10921 cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa cggcaaaagc tggtagcctg
    10981 agcgttgcgc tagaagtcat gatctatttt ggacttgttc ttctcggtgc gatgagtttg
    11041 aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
    11101 ttaggtaact tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc
    11161 gctatgggac tcgttgtttc ttttgcgcaa gatttccata aactgttccc taaagtcagt
    11221 tatatgacat ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggttta
    11281 gacaatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg
    11341 ttgatcttgc tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta
    11401 acgatgttgt ttgctgctgt tcctgccgta ttggatatgc tggcaaattc gcctgcttta
    11461 gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt
    11521 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcctactg
    11581 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
    11641 taaccaaatc aaacacaatt taacataaga aatattaaac aggaaaaaac cagctagatg
    11701 aaagaatcct attctttcgt ctagctattt tttagttctc ttttttacgc gcacaaaaaa
    11761 actgcggaca atgattgtcc gcagtttaaa tgggctctat aatatctagt gttggtgacg
    11821 ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac cttgaaatcc
    11881 tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt
    11941 ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc
    12001 gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac
    12061 cagaggaatg cttccattgt cactatcaaa ataagcaaac gatcattaaa tggggctgga
    12121 aaaaagtatc catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca
    12181 aacagcgctt caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg
    12241 atcgtcgctg ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac
    12301 ctctttctat gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa
    12361 tacttcgcag ccttcggcct aaaaccgttt ctttgaacca gccgttacca gaagtggtgt
    12421 gtttcgatga attcaaatcg gtgaaaaacg tctcgggagc catgagcttt gtcatgatgg
    12481 acggcaagac ccatcaactg attgatattg tggaaaatcg gcaactaaat cctttacgcg
    12541 actatttctt gcgctaccct agaaaagtcc gtgaacaagt acgtctagtg gtttcagatt
    12601 tctatacacc ctatcgcaca ttggtgaaag agtgctttcc gaatgcccga atcgtcgccg
    12661 atcgctttca tattagccaa cacatcggtc gggcctttac caaccaccga attcaagtaa
    12721 tgaagacctt taaaaaaagg gatcgtcggt cgaagcactt aaagaaatac tggagactac
    12781 ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag
    12841 atcatctgac agaagcagag atagtcgatc ggctattatc ttatgatgac tccttaaagc
    12901 gaggctacga agtctaccag gattttcttt ccgctatcag aagacaagat gttcctgaat
    12961 tcgtcgcgct cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca
    13021 cgaccttcaa gaaatatcga acagaaatca aacgagcgtt gcgagttccc tattccaacg
    13081 gccctataga atgtttgaac aaccatatca aagtactaaa acgaattgcc tatggcttta
    13141 gaaactttca aaactaccaa gaacgaatct ttttatatcg aggaaaatat tttaagaaaa
    13201 ctaagaacac tacccaactg actaaagcta gaacaacaac acggctagac aagatagctg
    13261 tctagccgtg tgagtatctc gattattcta ttgaat
//
LOCUS       DACCVU010000078        13243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCVU010000078 DACCVU010000000
VERSION     DACCVU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.078"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            175..1098
                     /locus_tag="IVW29_001980"
     CDS             175..1098
                     /locus_tag="IVW29_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7839459.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1116..2357
                     /locus_tag="IVW29_001981"
     CDS             1116..2357
                     /locus_tag="IVW29_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7839460.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2491..3837
                     /gene="gor"
                     /locus_tag="IVW29_001982"
     CDS             2491..3837
                     /gene="gor"
                     /locus_tag="IVW29_001982"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7839461.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4772..5725)
                     /gene="menA"
                     /locus_tag="IVW29_001983"
     CDS             complement(4772..5725)
                     /gene="menA"
                     /locus_tag="IVW29_001983"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7839462.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5738..6841)
                     /locus_tag="IVW29_001984"
     CDS             complement(5738..6841)
                     /locus_tag="IVW29_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7839463.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6931..7866)
                     /locus_tag="IVW29_001985"
     CDS             complement(6931..7866)
                     /locus_tag="IVW29_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7839464.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8168..10102
                     /locus_tag="IVW29_001986"
     CDS             8168..10102
                     /locus_tag="IVW29_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7839465.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10559..10975
                     /locus_tag="IVW29_001987"
     CDS             10559..10975
                     /locus_tag="IVW29_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7839466.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10989..11555
                     /locus_tag="IVW29_001988"
     CDS             10989..11555
                     /locus_tag="IVW29_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7839467.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11578..12564
                     /locus_tag="IVW29_001989"
     CDS             11578..12564
                     /locus_tag="IVW29_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7839468.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12719..>13243)
                     /gene="scm"
                     /locus_tag="IVW29_001990"
     CDS             complement(12719..>13243)
                     /gene="scm"
                     /locus_tag="IVW29_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7839469.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         4124 a         2400 c         2733 g         3986 t
ORIGIN      
        1 aagaggttgt gacaagattt ttgtcacaat ctctttttat atttggacag aatatatcga
       61 actttatttt gattttttgt gaaataattt tcaaaaatcc gactttaaat agaagaagta
      121 ttggaaagtt tttagagcta tgctactatt atgaagttgt aggaggagaa aattatgatc
      181 gaactcataa ccacagtaga aactattgaa caagcagaac agttgcttgc tgcaggtgca
      241 gatacactat atttcggtga agagcaattt ggattgcgtc tgcctgcttc atttacacga
      301 gaagaacagc gacaattggt agaattagca cacaagtatg gtaaaaaagc gaatattgct
      361 gtaaatggga tcattcatcc tgaaaaaatg aaaaatgtac ctgaatattt ggctttttta
      421 aaagaaataa acgtagatgt gatcacagtt ggtgatacag gtattattta tacgatgaaa
      481 aaaaatcctg aattgcgtat accgtatatt tatgatgctg aaacattagt aacgagcgca
      541 cgtcaaatca atttctgggc gaaaaaaggc gcagttggag cagtacttgc tcgtgaagtt
      601 ccttttgaag agatgaaggc gatggaagaa aaactcgata ttcctgtaga aacccttgtg
      661 tatggtgcta catgcatcca tcaatccaaa cgtccactgc tacagaatta ctataactat
      721 acgaaacaag acgaacaaaa agatcgtgaa agaggtcttt ttatctcgga acctaaaaaa
      781 gaagagacac attattctat ttatgaagat agtcatggca cacatatttt tgccagcaat
      841 gatctgaatc tctcgaatga attgaatgaa ctttatcagc atcattactg tacatggaaa
      901 ttggacggat tatttactcc aggcgaaaaa ttcgtaaaca tcactcgact attcgccgaa
      961 gcaaaaaaac aaattttaga aggtagctgg gatcaagaaa aagcggaaaa aatgactagc
     1021 gaaatcagta aattgcatcc tgaaaaccgt gaattagata caggattctt ttatattgat
     1081 ccttctgcta tcaaataagg agtatgatga aagaaatgac agtaaaaaga aaattgaaac
     1141 gaccagaaat cctagcgcca gcaggaacac tggaaaagtt aaaaaccgcg atccattatg
     1201 gagcagatgc agtatatatt ggcgggaatg catatggttt aagaagccgt gcaggtaatt
     1261 ttacttatga agaaatggct gaaggagttg cttttgcaaa agaacatgat gcgaaggtat
     1321 acgttgctgc taatatggtc acccatgaag gaaatgaaga tggagcaggg gaatttttcc
     1381 gcagtttgcg tgatgtcggt atttctgcag tgattgtttc tgatccagct ttgatcgaaa
     1441 tttgtgcgac agaagcccca ggtctgccga tccatttatc cactcaagct tcagcaacta
     1501 attttgagac attggaattc tggaaagaag aagggttgga acgtgtcgtt ttagctcgtg
     1561 aagtttcaat ggaggaagtg gcagagattc gtaaaaatac ggataccgaa atcgaagcat
     1621 ttatccatgg tgccatgtgt atctcttatt ctggtcgatg cacattgtcc aaccatatgt
     1681 cgatgcggga tgcaaaccgt ggcggatgtt ctcaatcttg tcgctggaag tatggattgt
     1741 tcgatatgcc ttttggtcaa gaacgaaaca gtttaactga tacaggcgaa gtggaagaag
     1801 aattctcgat gagtgcagtg gatatgtcaa tgatcgaaca tattccagaa ttgattgaaa
     1861 acggcgtaga cagcttcaag atcgaaggaa gaatgaaatc gatccattat gtatctacgg
     1921 tgtcaaatgt ctataaagcg gctgtagaca gttatatgga agatcctgaa aactatgtct
     1981 gcaagcaaga atggatcgac gagttatgga aagtagcaca acgagaatta gctacaggtt
     2041 tttactacaa tacaccaact gaaaacgaac aattattcgg tgaacgtcgt aaaatcccag
     2101 tatataaatt tgtcggtgaa gtattatctt atgatgaggc aacacagatt gctacgatac
     2161 gtcagagaaa cttgttttct gtaggagatg aaatcgaatt ttatggtcca ggattcagtc
     2221 atttcagtca aaccgttgaa gttatgcata atgaagaagg cgaatccatt gaccgagcac
     2281 caaatccaat gatgatttta actatgccag tcgcacagcc ggtgaaacct ggagatatga
     2341 tccgaaaacg taaataagga aaaagctgat atatccctgt tttaagcatc ttcgcataca
     2401 aaattcaaga aaagtgttac acttgttttc tgtagaaaaa ggatcgttaa ataaaggttt
     2461 tgattgatct cttcaataag gaggaacaaa atgcaaatat atgattacat cgtaattggt
     2521 ggcggctctg gcggtattgc ttcagcaaac agagcaggga tgcatggcgc aaaagttttg
     2581 ctgattgaag cgaatgaatt gggcggaaca tgtgtcaacg tcggttgtgt acctaaaaaa
     2641 gttatgtggc aagcaagtac gattttggaa acaattgaac gagatgcctc aagttacggt
     2701 atccaagcag atttaaaaaa tgttgatttc gccggtctag tagaaagacg agaaaaatat
     2761 attgattttc tacacggtgc ttatcaaaga ggattagata gcaatcatgt cgaggctata
     2821 aaaggttacg ctacttttat cgatagtcaa acgatcgaag taaacggtga aacctatcgt
     2881 gctcctaaaa tcttgatcgc tactggaggc cgtgcaagta aaatgaccat tcctggtgga
     2941 gaatacgcaa ttgattcaaa tggatttttt gaattaacag agttacctaa gagagcaatc
     3001 gttttaggtg ctggatacat tgcggctgaa ctttcaggtg tattttgtgg tttaggtagt
     3061 gaagtcatgt gggcatatcg aaaagaacga ccacttagaa cgtttgataa aatgctttcg
     3121 gacaatttga tccaaatgta ccaagaagct ggaataaaaa cttacgcaca tcatattgct
     3181 aaagaaatta ctaaaaacaa tgacgaatat acggtgctat ttgaaaatgg tgaaacctta
     3241 actggtgatt gtgtactttt tgccggtgga cgtgtaccga ataccgaaaa attaggtctg
     3301 gaaaatacaa atgtggaatt ggacaaaaaa gggtttatca aagtagataa ataccaaaat
     3361 acaacggatg aacatatttt tgctgttggt gatgtgatcg gtaaattaga tctgactcct
     3421 gttgcaatcg cagctggtag acgtttatca gaacgtttat ttaatggaaa gaaagattct
     3481 tatcttgact ataaacttgt tccgacagtc gtctttacgc atccaccaat tgcaacaatt
     3541 ggtttgacag aagaagaagc attggaaaaa tatggcgaaa atgaattgaa agtttatcgt
     3601 tcacgcttta ccccaatgta ctttgcatta aatgattatc gccaaaaatg cgaaatgaaa
     3661 ttgatctgtg tcggaaaaga agaaagaatc gtcggcttac atgctattgg tgttggtgtc
     3721 gatgagatgc tgcaaggttt tgccgtggct atcaagatgg gtgcaactaa agaagacttt
     3781 gacaatacag tagccatcca tccaacagga gcggaagaat tcgtaacgat gcgataattt
     3841 aatattaaca gttccaacag ctttttattg aacaaaccat gtaaaaccag aaaatcagta
     3901 tactgatttt ctggtttttt gttttttagt tatttatgta agattgcttc gataattctc
     3961 aaaacgaaat aagtagggaa gttggagatt ttgattatct tcgtaactga cgaagagtaa
     4021 atgaatccat ttggtatgca ctcttaagtt caaaatattg gtatcgatgt ttaaaagaaa
     4081 tagaaatgta tcgaagaaca gatgtttaaa aaagtgtgac tttgtataaa taaattagaa
     4141 gaagcaaacg aggatgttaa aagaatacca tatagttacc aaaccaagaa agttctcgtc
     4201 aagttcgctg gtacgaatca aatcaagtga aaaaagggac tgtgaaaaaa attgtgtcat
     4261 agttagtcac tttttccgaa caaatagtgt tcaatcagct gtccttcggt attagctcaa
     4321 aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc aggacaaaac
     4381 actgatttta caacctctga taaacggtgt tcaaaagcca gtttctcggt attaagccaa
     4441 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaag
     4501 cgactttttc acaacctctg ataaacggtg ttcaaaagcc agtttctcgg tattaagcca
     4561 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
     4621 gcgacttttt cacaacctcc tgataaacgg tgttcaaaag cttgcatctg cggtaataag
     4681 cccaaccaaa cgaaaaatat aaggagctat ttttatttgg ctgttcttat tacttgatgc
     4741 agggcagttt tttcacaacc tcttcatgct gttaaatggc ttgcataatg attgttagta
     4801 atagccccaa tacataactg gcattgaata agaccatatt tttgatggaa tagctgaaac
     4861 tgataggctg gggtaacgtt tgcttgaatt gatccaaatg cttcttcaca attggcaagg
     4921 tcaagaaggt gaccaagatc ggccattcat agataccgct caacaacccc attaaaatca
     4981 cgaaatagca agcatacatc aatagctgga ataagactgt tcccgcagga cgtccgatat
     5041 aaaaaactag tgtatagcga tgattttcaa tatcacggtc aagatcccgc aaattattag
     5101 ccaacatgat attggcaatc gtaaacacca agggtaagga agctaatacg attgctgcta
     5161 tagcccaaag atttcctacc aaagcaaatt gtccagatga aaagttgact gtcagatcat
     5221 agacatctga accgaatgta ttgacataaa cgaccatggc aaaaattcca aggcccatag
     5281 tcaccccact gaaaatttcc cctaaaggca ttcgagataa aggaatcggt ccgaatgtat
     5341 agaatactcc aataaaacaa cagataaatc ccatcaaaag taaaagccaa cccgtattga
     5401 tcgttagaat aacgccgata acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa
     5461 tcaatacagg agatagattt ctgcggccaa tgacattttc ttcatactta tatacttcag
     5521 aatgagcctt ttcataatcc atgtaattat tgatagcggt agtcgctaaa tcaaaaacta
     5581 gcattccaat aaaaaataaa aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag
     5641 agaataatac ccctaccgca aaaggaaata cactagccac ctttgttcgt atttcaacta
     5701 cttctaaaaa gatttttagt gtcatggttc gtactcctta ttttagttca gaacggtcac
     5761 ccatcgtgta atgggagtcc tccgataatt tgaaggtgtc tttgattgga tcggtcacat
     5821 atactttatc gtcttttgtc acgaagatcg cttccgtccc gttattcaat tcgctttcga
     5881 cgtattccag tccttttttc acacccattg aaaagactgc tgtagacaac ccgtcaccat
     5941 caattgattt atctgttatg atcgtgacac ttgcgatatc attgtcgaac ggataacccg
     6001 tttttggatc aaacaaatga tggtattttt tgccatctac ttctaaataa cgctcgtaaa
     6061 ttccagaggt aactaatgtt ttgttccgtt ctttaataga acctaaaaca gatccgcgag
     6121 ccatgttcgg atcttggatg ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc
     6181 ctaagacata gacattaccg cccaagtcaa cgatggcagt ggtcactcct tgttttttca
     6241 agactttcac tacttcgtcc gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca
     6301 ttcctttttc ttttagataa acagtttttt ctttgtcatt tagttcgatc ttatgataat
     6361 ccactaattt caatgcctga tcgatttctt cttgggaagg ttttctggcg tcatcaaaac
     6421 cgattcgcca taattgtgtg attgcaccaa tcgccatatc gaatccgcct tgagaatctt
     6481 ggctatattc gtaggcccgc ttgactaacg tgtagacgtc atcagaaact tttactggtt
     6541 tgatcccagc ttgctcattc acttcatcga tctcagaacc tttttgattg atcgtgattt
     6601 tatcaccaag ttcttttact cgatcgaagg caggtttcaa tgctgattct ttgccttcat
     6661 catacacacg gatacgaacg taagtcccta acaagaactg ttcatcagaa tatggatctt
     6721 tcaatatctt ggtagctgct gaactttcag tggttgtttt atttgctgag ttgctgcagc
     6781 cagttagcac cataaaagag atgcccagtg tgattaagga aaatagtagt tttttccgca
     6841 tctttttcct ctcttcattt tgaaaggtga gacgactgcc taatctggct atcgtcccac
     6901 cctccaactt ttattagtat gattatgtga ttatttttct gttacgatat tatctacttc
     6961 gattgtttga gtgtcgcctt tttcagctgc attcactaat tgttgtgcgt acatgatgaa
     7021 tgaatgtgag ctgtgtgtag cacctgtaac aacttctaca ttagcaggtg agccatcttc
     7081 ttcgcccatt gctttgacga attcgtcgtt cagagtaggg atgtaagttt ttggttctgt
     7141 accgcttttt gctttcatat tttcattata ttctgtatca tcttgttttg atttaccgtc
     7201 tttgtcgata taatcaaagt ttgattcagt tactttgccg ccagcaactg tcatttcgaa
     7261 ttgcacacgg tagccgttag agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa
     7321 atcagcacca ttgtcgattt cgattgtatc tgtgttacca gcttgagcag cttggatcaa
     7381 ttgttgtgcg tagttttgga agctttcaga tgagtgagta gctccagtta cgacttccac
     7441 accgcttgcg ctttgtgctt ttaagaattg gtcattcaac tcaggaatgt atttttctgg
     7501 gtttgtgcca gattttgctt ccatgttttt gttgtattct gcatcttttg tttttgattc
     7561 gccgttttca ttgacgttat catattttga ttcagtgatt ttgccgtctt ttacaaccat
     7621 ttcaaagact gcacggtaac catttgaata atttttttct tctaatttat atgtgccgtc
     7681 ttgcaaatct ccgccagcta caacttttgc agatgattct gttgtgcttt ttgcagtcga
     7741 agattgtgct gtagatgatt cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca
     7801 agcgcctaac actagagctg aaaatgctaa tacggcaaat ccagttacaa aacgtttttt
     7861 cttcatttta ataccccacc attccatttc tagtccacaa tttttctttg tgattcatta
     7921 tacattctaa tctttttagc ctataagtca actatttttg ataaataacc catgtaatat
     7981 ttcaaacgtt ttctttcagc aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa
     8041 aatccaaaag gagttagaca aaagaacaga acatctatat aatatatgga gattgtgtaa
     8101 taagtatcaa actaaaagaa ttatcatata ttcattatat agatagtcta taaggagcgg
     8161 atgatcaatg gcaaagcaaa atattgtcgt tgtaggagca ggatatgccg gtgtttcagc
     8221 tacaaaatac ttggcaaaga aactgaaaaa agaagatgta gcaattactc tgatcgatcg
     8281 tcattcgtac catactatga tgaccgagtt gcatgaagta gctggcggac gtgtagaacc
     8341 agaagcaatc caatacgatc tgcagcattt gtttgctcgt caaaagaatg tacaacttgt
     8401 cacagacact gtaatcggta tcgataaaga acgaaaaatc gtcaagacca aattaggttc
     8461 ctatcctttt gatcagctga tccttggaat gggcggagag ccgaatgact ttggtacacc
     8521 tggtgtcaaa gcacacggat tcaccctttg gtcatttgaa gattctgtaa ggattcggga
     8581 acatattgag aagacagtag caaaagctgc attggaacca gatgatgaac tgcgtaaagc
     8641 catgctgact ttcgttgtct gtggttctgg atttaccggt attgaaatga ttggggaact
     8701 gatcgactgg aaaaatcgcc tagccaaaga ttataaatta gatccaaacg aaatcacact
     8761 gatggtcgta gaggcaatgc cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa
     8821 agctgaacgc tatttgcgta agcacaacgt ggaattatta ttgaatgcac caatcgtaga
     8881 agtggctgct gatcatatca aattgaaaga cggacgcaac attccgactc acacactgat
     8941 ttggacagct ggtgtcaaag gaacttctga cgctgctgac tttggattag aatcagcacg
     9001 cggacagcgg ttgatcgcca atgaatatat gcaggcaaaa ggctacgaag ataaaaacat
     9061 ctatatcatt ggtgatttag tcttttatga agaattccca ggaacaccga caccgcagat
     9121 cgttcaagct gctgaacaaa caggacacac agccgcagcg aatatcgtgg cttcgatcaa
     9181 aggcggagaa aagcataaat ttaaaggaaa ctatcaagga tttatggttt ctattggtgc
     9241 caaatggggg gtagctaacc tatttgataa gatccattta agcggatttt tagctatcgt
     9301 aatgaaacat atcgtgaatt tgaaatactt ctttgatatt cgttctggtt attatatgtt
     9361 ccaatattta atgcatgagt ttttccatat caaagatgat cgcaacgtga ctcgaggaca
     9421 ttcttctcgt tacggcaatg tcttatggag tgtgccactt cgtattttct atggtttagt
     9481 ttggttcgtt gaagcttcca aaaaaatcat tgggaatggt gaagtgttca aaccaagtac
     9541 ttggtttggt gaaggatcat ggttcactgg aaacagtgtg ttcccttgga catgggaata
     9601 caatgtaaca gatgttgcca gtggtgcttc tgctgctggt gatgcaacca cagctgccag
     9661 cggagcaggg gcaagtgccg gagaagcagc gactcaagct gcacattttg gtttgagtta
     9721 tgcatacggt gaagaaccaa tggctgtgat tgagaagtca ccagactggt tcacaaagat
     9781 catgcaagtg atcatgccaa acaatgatgt agcaatgttc ttccaaaaat tcatggtatt
     9841 ctttgaaatt gccttggcat tagcattgat tgctggatta ttcacatggc tatgcagtgc
     9901 gacaacgatt gtattggtga tcacgttctg cttatcaggc atgttttact gggtaaacat
     9961 ttggttcatt tttgttgctt tcgcattgat gaacggttcc ggacgtgcga ttgggttgga
    10021 tcgctgggta attccatggc tacaacgtac actaggtaat tggtggtacg gtagaccaag
    10081 ttcccagtac ggcaagaaat aaatagataa agaacgttat ttgaatgcaa gatttcaata
    10141 gaaaggggct gagacaaacc gtcacagcct cctttttatg taaaattaaa ggaataagat
    10201 gcccgagata agcctccatg agcaagacac aggtgtcaca acctctgtta caacctcttg
    10261 ttataaacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaact
    10321 tgaaaagtaa ttttcgtaaa ttccttctta tttcttgaag ttgcccactt ctgtcacaac
    10381 ctcttgttat aaacggtgtt ccacctgctg cttctcggta ataagcccaa agaattagaa
    10441 aacatggaaa gctgttttct attcttcgtt cttattactc aaagcaagac acaggtgtca
    10501 caacctcttg tttttatgct aaaattgggt gagttaatct ggaaaaagga gaacaaaaat
    10561 gagccttaaa gaatttgctg aaaaaagtct gctcaaacct tgggatgttt ttattatttt
    10621 gcttttgact gttctttctt ttttaccagt catgattttt ggtcttcagc aaaacaccgc
    10681 aacagcagaa aaagaagcgg tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt
    10741 gatggctgga gaagagagca acacatatac ttacaaagat gaacacggag attataactt
    10801 gatcgagata gatggcgatc gagtacggat aaaagaagca gattgcgatg accagatctg
    10861 cgtacgtcga ggctgggcaa cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt
    10921 agtgatcgaa gtccgttcaa cagatggagg tgacgaagac agtctgattt attagacctt
    10981 cacacaccat gagtaaatta caaaaaatga tttatatttc gctacttgtt gcacaaggag
    11041 tgatcattgg tttattggaa aatatgatcc cttatccttt ttcttttgcg cctggtgcaa
    11101 aactaggctt agccaatcta atcacgatcg ttgccatttt taccatgaaa aaacgagaca
    11161 gcttcttact actttggcta cgactattcc tgactacttt gctaggcgga acgatttcca
    11221 cattccttta tagtatgagt ggtgctttgc ttagttattt tggaatgtta ttggtcaagc
    11281 aattaggacc aaaacgtgtc agtatcatcg gaattagtgc ggccggcggt ttcatgcata
    11341 atgtcggaca gcttttgatg gcttctttca ttgcacaatc atggacagtc atgttgtact
    11401 tgccagtcct ttcagtttta gggattttat cagggattgc aatcgggatc gcagcgaatt
    11461 atcttttacg acatgtgcag acacttcaac gattccaaaa agattatgag cagggaacca
    11521 aacaaaaatg gagcccaatg tttaccaaaa aataatcgtc acacaaggag tttttttatg
    11581 aagctacatc caatgtggga aaactatcct gctttaagta aagaattgac agaaacacta
    11641 aaacggatgg aagaaactgt ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa
    11701 acgatccatg ccggcggaaa gctgttgcgt cctgcctatc agctattgtt ctcacaattt
    11761 ggaccagagc aagatcgaga caaagccatc gctttggcag cttctatcga aatgctccat
    11821 acggccacac tgatccatga tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct
    11881 aatatccgta gtcagtttgg taatgtcgtt tctgtctatg caggagacta tttatttgtc
    11941 tgctgtttta aactattgtc tgattactcc acttccttga aaagtctcca attgaattct
    12001 cgcagtatgg aaaaagtatt gaatggcgaa ttagggcaga tggatgatcg ctataattat
    12061 gaactttctg taaaagaata tttggaaaat atttcaggta agacagctga actgttccaa
    12121 ttaagttgtt ctgttggcgc ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca
    12181 ggagatatcg gactttctat cgggatggcc tttcagatta tcgatgatat tttagattat
    12241 acaaaagaga gccaggaaat cggcaaacct gtactagaag acatgcgtca aggggtctat
    12301 tctttaccgt tgatctactc tttgcaaaaa aacaaaccaa agctactacc ttatttagag
    12361 aagaaagcgg caatgacaga agaagatgtc gacgccgtac gcaaaatcgt tgaacataca
    12421 ggaggcgtgg aagaagctag gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa
    12481 ataaagaaat tacctgccac ctctcttcga acaaaagaga atctcttttc ccttacccaa
    12541 ctgattttgg atcgcaaaga ctgaataggg aaaggttcga tacgtaaata aatttctcag
    12601 aaggaaatct acgtgttttt tgacaaattg aattgtagtt cttgtattta tgataaaaag
    12661 caaaaataaa gagcaacgtc cgttagcgac gttgctcttt tttatcttga atattttttt
    12721 accttttcga tttcttataa ctcataaaag tgattcttaa accgattaca agtaatataa
    12781 cgcctgcgag tgttatccaa ttattcgatt gacttccagt tttaggaagt gctctttttg
    12841 atttgtttcc attgtcattg cttgttttat tagttaagtt aaagttcgtc ctgttatccg
    12901 aacttgtttt tgagaaggag acacttgcat gacgtgtatc gcttgtggta gacgacgtgt
    12961 ctgtactatc gcttgaagtt gatgaatctg agctatcact tgtggtagag gatgtatctg
    13021 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt agtggtagat gaatctgagc
    13081 tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg tccgtgctat
    13141 cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac
    13201 ttgtggtaga ggtagtatct gtactgtcac ttgaagctga tgt
//
LOCUS       DACCVU010000079        13114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCVU010000079 DACCVU010000000
VERSION     DACCVU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.079"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            79..1818
                     /gene="yfcC"
                     /locus_tag="IVW29_001991"
     CDS             79..1818
                     /gene="yfcC"
                     /locus_tag="IVW29_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7839470.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1975..3159
                     /gene="iadA"
                     /locus_tag="IVW29_001992"
     CDS             1975..3159
                     /gene="iadA"
                     /locus_tag="IVW29_001992"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7839471.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3270..3473
                     /locus_tag="IVW29_001993"
     CDS             3270..3473
                     /locus_tag="IVW29_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7839472.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3574..4236)
                     /locus_tag="IVW29_001994"
     CDS             complement(3574..4236)
                     /locus_tag="IVW29_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839473.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4249..5751)
                     /locus_tag="IVW29_001995"
     CDS             complement(4249..5751)
                     /locus_tag="IVW29_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839474.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6299..7978
                     /locus_tag="IVW29_001996"
     CDS             6299..7978
                     /locus_tag="IVW29_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7839475.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8105..8584
                     /locus_tag="IVW29_001997"
     CDS             8105..8584
                     /locus_tag="IVW29_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7839476.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8803..9483
                     /locus_tag="IVW29_001998"
     CDS             8803..9483
                     /locus_tag="IVW29_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7839477.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9491..10822
                     /locus_tag="IVW29_001999"
     CDS             9491..10822
                     /locus_tag="IVW29_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7839478.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10837..11799
                     /locus_tag="IVW29_002000"
     CDS             10837..11799
                     /locus_tag="IVW29_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7839479.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11910..12248
                     /locus_tag="IVW29_002001"
     CDS             11910..12248
                     /locus_tag="IVW29_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7839480.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12327..13034
                     /locus_tag="IVW29_002002"
     CDS             12327..13034
                     /locus_tag="IVW29_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7839481.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4164 a         2318 c         2878 g         3754 t
ORIGIN      
        1 catttttact gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa
       61 agaaaggaag aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc
      121 actaaattat cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg
      181 atactgactt ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat
      241 gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac
      301 gagctggata agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa
      361 cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa
      421 aatcttacgc aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag
      481 tacggcgaaa atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga
      541 acagatgatt ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga
      601 gataagtatg gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt
      661 ggtattatta tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact
      721 aagggactgg agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct
      781 acattcggga tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt
      841 gcgttaggat atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga
      901 aatgcgacct cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc
      961 cccgtgttgt ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc
     1021 gctggttata tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact
     1081 tacaaatcag atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa
     1141 ttcatgcttg ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt
     1201 tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg
     1261 ggattagttg ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac
     1321 atcgcttctt ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt
     1381 atggccaaag ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg
     1441 aatacgattt tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct
     1501 tgggcgatgt acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa
     1561 gcagctttga ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa
     1621 gtagcagttc ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca
     1681 gccagcttga ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc
     1741 caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt
     1801 gcaataggtc tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat
     1861 aaggactcca agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg
     1921 gattattttt taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa
     1981 atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt
     2041 gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa
     2101 gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc
     2161 catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg
     2221 agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa
     2281 ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc
     2341 acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc
     2401 aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac
     2461 cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga
     2521 ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag
     2581 ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct
     2641 actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt
     2701 ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa
     2761 gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc
     2821 acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta
     2881 gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa
     2941 gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg
     3001 aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat
     3061 aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa
     3121 gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt
     3181 tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat
     3241 caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata
     3301 tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga
     3361 gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa
     3421 atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac
     3481 ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg
     3541 ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt
     3601 attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat
     3661 gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga
     3721 agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca
     3781 gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt
     3841 ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata
     3901 attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa
     3961 ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct
     4021 taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc
     4081 tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc
     4141 gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc
     4201 ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag
     4261 gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact
     4321 gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa
     4381 ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg
     4441 ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt
     4501 ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac
     4561 gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa
     4621 caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg
     4681 ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagt
     4741 cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga
     4801 ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc
     4861 ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat
     4921 cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt
     4981 attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc
     5041 ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt
     5101 gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt
     5161 gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc
     5221 agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt
     5281 catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga
     5341 actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga
     5401 ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc
     5461 cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga
     5521 accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt
     5581 cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat
     5641 cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga
     5701 acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat
     5761 ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct taaatcttat
     5821 gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc
     5881 aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg
     5941 aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca
     6001 attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact
     6061 tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc
     6121 caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat
     6181 tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt
     6241 agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt
     6301 gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt
     6361 cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga
     6421 gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg
     6481 tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt
     6541 gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt
     6601 gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca
     6661 tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc
     6721 agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt
     6781 taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat
     6841 cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc
     6901 tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg
     6961 gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc
     7021 agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg
     7081 cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa
     7141 agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga
     7201 acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga
     7261 aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa
     7321 aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg
     7381 cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac
     7441 atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca
     7501 tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata
     7561 taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg
     7621 attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga
     7681 cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc
     7741 tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag
     7801 cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag
     7861 caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg
     7921 ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac
     7981 aaaaagagta agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac
     8041 aaataatttt tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga
     8101 atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca
     8161 accacttgat tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg
     8221 gattgatcca gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat
     8281 catcgcattg attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa
     8341 agatcgtaaa gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca
     8401 cggagcaaac acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga
     8461 cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct
     8521 tcggataaaa gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag
     8581 ttaagggacg agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc
     8641 ttggtacttg gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca
     8701 atccaatgaa aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg
     8761 tttttaataa aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat
     8821 ggacattcct gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt
     8881 attacaggaa atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg
     8941 attactcatg gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa
     9001 gccacaatta tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca
     9061 ttcggtatga atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat
     9121 gcacagcata gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc
     9181 ggaattatta ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc
     9241 agtgatctag gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc
     9301 gataactata cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag
     9361 aaagtagttc caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt
     9421 gtagagttac tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg
     9481 taggcagaaa atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt
     9541 tggcaatcga atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc
     9601 gtaccgtgca atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg
     9661 gaccatccgt atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt
     9721 cgtcaggatc attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa
     9781 taaattcgtc atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc
     9841 actaatgatc gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc
     9901 agtattgatc acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct
     9961 tgaaatgccg atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg
    10021 agcgttaagc gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc
    10081 agctgaaaag actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc
    10141 agaagaaacg cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat
    10201 tgttacggca aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac
    10261 gaaagaacca atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat
    10321 ctgggatggg ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat
    10381 tacccggcaa gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga
    10441 tacgatttcg gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa
    10501 tcgtttggaa aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg
    10561 aaccatttct tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt
    10621 gatgaaccaa aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat
    10681 ccaattggaa agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc
    10741 taaactggat atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg
    10801 tcaagtaatg gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta
    10861 gatacaataa aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat
    10921 gcattaggat cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa
    10981 atacgtcaag taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt
    11041 gaagatgtct tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga
    11101 atcagtgatc aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat
    11161 gatgaccctt atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg
    11221 attgcagaat ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg
    11281 ttactttatg caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc
    11341 cgtacattag agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac
    11401 agagagattg aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat
    11461 atccaggtgg atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa
    11521 tatggaatcg atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa
    11581 gtgctggctt gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt
    11641 tctaaggggc cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta
    11701 gcaagtggtg cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa
    11761 gatatatgcc aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc
    11821 ttttgtagta aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg
    11881 ctcactaaag attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct
    11941 cagagtacac atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga
    12001 acaaagaaga acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa
    12061 accgtctgtt agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt
    12121 caggtgccat taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata
    12181 atatcgattg caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa
    12241 agaattaaag aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa
    12301 agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc
    12361 cgacatttct tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca
    12421 atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag
    12481 cagcacaata tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg
    12541 gagatctcac agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg
    12601 cacagtctac ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac
    12661 aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact
    12721 ttggttcgat gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta
    12781 cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat
    12841 taccagtcga atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac
    12901 tgacggaaga agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg
    12961 ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca
    13021 accgaagaga ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga
    13081 gagttagctg attgaatacc atttatttgg aata
//
LOCUS       DACCVU010000080        13078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCVU010000080 DACCVU010000000
VERSION     DACCVU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.080"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            86..1066
                     /locus_tag="IVW29_002003"
     CDS             86..1066
                     /locus_tag="IVW29_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7839482.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1188..2606
                     /locus_tag="IVW29_002004"
     CDS             1188..2606
                     /locus_tag="IVW29_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7839483.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2677..4146
                     /locus_tag="IVW29_002005"
     CDS             2677..4146
                     /locus_tag="IVW29_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7839484.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4156..4623
                     /locus_tag="IVW29_002006"
     CDS             4156..4623
                     /locus_tag="IVW29_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7839485.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4659..5531
                     /locus_tag="IVW29_002007"
     CDS             4659..5531
                     /locus_tag="IVW29_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7839486.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5613..8147
                     /locus_tag="IVW29_002008"
     CDS             5613..8147
                     /locus_tag="IVW29_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7839487.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            8303..9076
                     /locus_tag="IVW29_002009"
     CDS             8303..9076
                     /locus_tag="IVW29_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839488.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            9069..9860
                     /locus_tag="IVW29_002010"
     CDS             9069..9860
                     /locus_tag="IVW29_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7839489.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            9870..10361
                     /locus_tag="IVW29_002011"
     CDS             9870..10361
                     /locus_tag="IVW29_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839490.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            10379..10789
                     /locus_tag="IVW29_002012"
     CDS             10379..10789
                     /locus_tag="IVW29_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839491.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            10793..12193
                     /locus_tag="IVW29_002013"
     CDS             10793..12193
                     /locus_tag="IVW29_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7839492.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            12216..12590
                     /locus_tag="IVW29_002014"
     CDS             12216..12590
                     /locus_tag="IVW29_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7839493.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4530 a         1768 c         2637 g         4143 t
ORIGIN      
        1 aaaaggggaa tgagagagtc aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt
       61 ctatctacaa ccttgttgtt taaaaatgaa aaaaattgga attagagaag ttgcgaaaga
      121 ggcaaatgtt tctcctacga ctgtgtcgag ggtattgaat gatcgtggat atttgagtga
      181 ggaaacaaaa agaaaagttt ttgatgccat gaaaaaatta gaatactatc ctaatgaact
      241 ggcgagagca ctttttaaaa acgaaacata taccgtaggt ttgatttttc ctacaattac
      301 taatccattt cacgctgagt taattcaaga tatcgagttc gagctttcca gcaggggata
      361 taaagtgttg ctgtgcaata gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat
      421 gttgagaaag aatcaggttg atggaattat agtaggaacg cacaataaag atattgcgga
      481 ttatgatatt ccagggctac ctattgtagc gattgataga agtcttggtg aaaatacagt
      541 tacagtatct tgtgataatt acaggggtgg ggaattagct actcaactac ttgtaaatca
      601 tggctgtcag aatatactgt gtattcgggg agatagtaag ataaagctac ccgcgaatag
      661 taggacttta gcatataaag atgttttgaa taaattcatg ctggaaccgt tagtcctaga
      721 agtaccattt gtaaaagaaa tcaatgaaaa aaatgaaata attaagacat atctggaaac
      781 tcatccaaat attgacgggg tttttgcagg agatgattta ttggcttctt tggcgattaa
      841 caacttagga agttcgggca aaagagttcc aaaagaggtt aaagtgattg gatttgatgg
      901 agctcaacaa acattgattt ataatccaga attatcaact attcaacagc ctatagatca
      961 aatatctaaa gtagcagtaa aaaaattatt gaatatgatt aagggcgaaa aggagacgga
     1021 tgaactttca cttccagtaa agctcataaa aaaaaataca gcataagtta aatcggcgaa
     1081 aaattccttc gtcgatttaa aaatattttt atgaaacggg ttgacatatg aaacgggttg
     1141 acatatatta gttgtgaaga taaaaacctt ttcactggag gaagaaaatg gagaaattat
     1201 taattaaaag caataatcat caactttact accaacctaa ggatgtttgg gtcggagatg
     1261 ttatgcctta cgggaaagaa ggaaaattct ttatttatca tcaacgtgat actagaaatc
     1321 cagttccttt tggagaaccg tttggctggg ctttagcaac tacgaaagat tttgttcatt
     1381 ttgaagactt tggggaatct cttttaagag gggatgacaa tgagagagac cagtttatct
     1441 acgctggttc tgtatttgag gcaaatggaa aggttcacgc attttataca gggtataatc
     1501 gtgaattctt agcgcaggga aagacatctc aagtgttgct tcatgctgag agtgatgatt
     1561 ttgttcattg ggaaaaatca gaaacagcat tggagttaga accacaagaa gggtatgata
     1621 ttcgaaactg gagagatcca tttgtcgttt gggatgatga aaaagaagaa tatttgctaa
     1681 ttttaggggc gcgaaagggt caggataaac atctacaaac cggacgttta gtggaattta
     1741 catcaaaaga tttaactaat tgggaattca aaggtgattt ttgggctccg ggattgtata
     1801 ccatgtttga aatgccagaa cttttcaaga ttggtgactg gtggtatctt gtctattcag
     1861 aatactcaga tggaaataaa atactctatc gaatgagtca aagtttggaa ggtccttgga
     1921 ttaagcctaa agatgaagct tttgatggac gggcttatta tgcaggtaga acagcttatg
     1981 atggaaaacg tcgagtccta tttggatggg taccaacaaa agatcaagaa aatgataaaa
     2041 acaattattt atggggaggt actttggttc ctcatgaagt ctatcaaaaa gaggattttt
     2101 cacttggggt aaaacccata gacgaagtgt ggaatgcatt cataaattta catgatattg
     2161 ccgatgtaga gatagaaaca gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg
     2221 gaactttgtt taatttagaa acaacaattt catttgatga gaatcttagt gacttttcac
     2281 tgagattata taaaaacctt gagacagatg aatcttacga atatcgcttc aatttagatg
     2341 aggggcgtct tacatttgat aaaaaccctt gttacccttg gtttcaatat atggataaag
     2401 gacttattag acctatatct ttaaagccta accaatctta taaactaaaa gtgattgttg
     2461 atgacaatat aatgacactt tatattgatg gaatcgctct taacgctcgt gtacatggtt
     2521 gttttggtca agcaattact attacagcaa caaatggatc aatgactgca ataaatacca
     2581 agttttcaaa tgaaataaag aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa
     2641 acatgacctg tcacatttaa aagggaggat aaatgaatgg actacaaaaa aattgccgaa
     2701 gatgtattgg aaaacgttgg aggaaaagag aatatctcaa gcgctacaca ctgcattact
     2761 cgtttacgtt taattcttaa agacaaaagc aaagcgaata tggaaatgct tgaggaaata
     2821 gaaggtgcaa aaggtgttct tttcaatagt ggacagttac aagtaatttt cggaacaggt
     2881 ataattgaaa aggtttacaa agagttcgtt gaattgacag gagctaagga agtatccgtt
     2941 tctgaaatga aagacgaagg agtcaataat tcaggaaata gacttcaacg agcttttaaa
     3001 gttttctcgg atatctttat tccgatcatt ccagctttta ttggggcagc aatggtttta
     3061 ggaattcgtt cgttgattac cactcctgga atgtttggaa tggatggctc attcgctgat
     3121 cacaatgtat tcctaaatga tttatccaaa tttctgggta ttattgcaac cacatttgat
     3181 tatttaccag ttttagttat gtattctgca acaaaacgtt ttggcggaaa ccctatttta
     3241 ggattacttg taggatttgt tatgattcat ccaggactag ccaatcggaa tgatttcgtc
     3301 atgggaacta taaagcctga ttattggcat ttatttggat taagcattcc agcagttgcg
     3361 tttcaagggg gtgtttttcc agcaattttg acttcttggt ttttagcaaa agttgagaaa
     3421 attacaaata aatattctcc tcaagtactt gcttacattt tagtcccaac tgtgacaatt
     3481 gttcttgcca actttgcact atttttagtg tttggtccat tggggaattt tatcggtaat
     3541 gttttaggtg gaatcattga tttcctatat atgaagatgg gtgcctttgg tgcttttgtt
     3601 tttgcaggcg cgttacaacc attagttgta acaggaacac aacatgctat tcaaggtatt
     3661 gaggcaaact tagtcgcaac aactggattc aattatatcc aacctatttg gtcagtttca
     3721 attattgccc aaggtggcgg ttgtattggt atgtatttac tctttaagaa aaaatctaaa
     3781 gatagagata ttgcgatttc aagctttatt ccaacactgg taggtattac tgaaccggct
     3841 atctttgctg ttaatttgaa atattcaatc gttccttttg tttgctcgtt tattggtgct
     3901 ggttttggtg gagcatttat gaaaattttt gatgtaaaag ccattggaca aggattgact
     3961 gtattgccag gtctgactat tgctaatcca gttcttcctt atatatttgg taatctcata
     4021 gcattcattt taccaatcat ttttattctc gcttatgata aatataaagt gtttatccct
     4081 gaagctaaac accgtaaatt tgcagaaaaa actgactcaa aaaaattgcc agttcaagta
     4141 aagtgagatg agagcatgtt aaattttttt agaaaaaatg aaataagcgt tgtagcacct
     4201 gcaaccggtg tttttgaaaa actggagaaa ttaagtgatc cagtcttttc aaaaggaatg
     4261 atgggacaag gatttgcaat tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt
     4321 tttgtttcaa gtatttttcc tacaaaacat gccttaggaa taaaaacaaa atcaggaatt
     4381 gagatacttg ttcatgtagg aatagataca gttgagctaa atggtgaggg atttgatatt
     4441 aaagttcaag aaggacaaaa agttaaggct ggggatattc ttatgaccat taattttgta
     4501 gtttttgaaa agaatcacaa gactaaagat gtcattgttg tttttccaga ctaccaagac
     4561 gaaattttca ttccgaaact tgaagaagta aaacaaggac aagaattgtt tgttcttggc
     4621 taatgataag taattaataa tagaatcgag gaaaatgtat ggtttttggg gctattgaag
     4681 ctggtggaac aaaatttgtt tgtgccattg gagataaaga tttgaacata tttgaaaaag
     4741 tcagctttcc cacgactaca cccaaagaaa caatgaattt agttttagat ttttttaatc
     4801 atcataaaga tgatttagaa ggtatcggag tgggatcgtt cggtcctatt gatattcatt
     4861 gtgaatctaa aacgtatggc ttcattacat caacgccaaa acttgcttgg caggattttg
     4921 attttattgg aagtttaaaa aagcaactta atattccaac tgcttggacg actgatgtaa
     4981 atgcctcttg ctatggtgaa tatgtttctg gaaatggcag aggcctttca agtgttgttt
     5041 actataccgt aggaacgggc ataggaggtg gcgctatcca aaacggaaac ttcattgaag
     5101 gttttagtca tccagaaatg ggacatatgc ttgtaaaacg acacaataaa gacaactttt
     5161 ctgggacatg tccattccac ggagactgtt tagagggctt agctgctggt ccggcaatcg
     5221 aaagaagatc agggaaaaag ggacaagaag tgactgaaaa tgacgttact tgggaaatag
     5281 aagcttatta cattgctcag tgtgcatata atacaacatt gatgttatca cctgatgtca
     5341 ttatatttgg tggcggagta atgaaacaaa aacacttaaa aaagaaagta caagataagt
     5401 ttattgagtt gatcaacgga tatgtcgcta ttcccgatgt tgacaattat attatgacac
     5461 caaaattgtt agataattct ggtattgtag gttgtttagc tttagcaaga gacgttaaag
     5521 aaaaatctta atttggaaaa aagatcgtct cgactatttg tttggttgat gcgatctttt
     5581 ttttcaatta gtaattgaag ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat
     5641 tagagtatat caaaaataat ttaaagtatt acgatttttc tgctcaagat attgcaatga
     5701 aattttgtat taaaagaaat gtcgcttccc actatttgaa tcagttattt tcagatggaa
     5761 aattactaaa gaatgattct gttagaccag taatgttcaa gtataatcaa caaaagccaa
     5821 aagactgctt ttctaagttt attggagcag acatatcttt gaaatccact atagataaat
     5881 gcaaagctac tgtaatgtat ccccctaatg gcctcccatt aataataaaa ggaaacagtg
     5941 gcgttggaaa aagtttttta gcaagtttga tctatcagta tgctcttgat agaaaagtaa
     6001 ttcataatga cgcaaaattt gttgttgtga attgtgcaga ttatgcaaat aatccagagc
     6061 tattgtcagc agttttattt ggatataaaa aaggagcctt tactggggct gaaaaagata
     6121 ctgcagggat gctatcttct gctaatggag gatacctttt tttagatgag gttcacaatc
     6181 taagtgcaga aaaccaagag aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac
     6241 ttggtgaaag tgtgcaatgg gagtatgcaa atgttcgcct tatcttagcc acaacagaag
     6301 ataaaaatac ctctttgtta gcaactttta gaagacgcat cccagctgaa atcactctgc
     6361 cagattacaa taatagaagt acatctgaaa aaattcaatt attatttgaa tttcttaaag
     6421 atgaagcaat tacaattgat tcaaaaatat tttgttcgat tgaacttttt actgatcttt
     6481 tatcgaaaac atttgaagga aatgttggag aattgaggaa tgaaatcaag cttctttgtg
     6541 cagaaggtta tttaggaaat aagagaaagt cttcaattta tttaggggac aaactagaca
     6601 gcggtttttg gatagatcca gaaattagta tagatttaaa aaaaatgttt cttaaatcac
     6661 tttctgaaat tgatttgatt gaactttttc aaaatcacat ttcaattgaa gaaagcatga
     6721 gtcaattaag agatagaata cttaaagagt gtgcaggttc ttctgagtat aattatttag
     6781 aaagtgacga atttatagca ttaagaaact atgttgttaa taaaatatca ccaattattg
     6841 attcgacagg tctatgttta ttagatgaat tcttggcgga tatcagtcta tttataatgt
     6901 ttattgatgt aattgaagaa acatcaagaa attttttgct gtctaagttc agaaaatttc
     6961 gtatgaaaaa tgataaacaa aaattattgg caaatgagat ttggagtagt ttagaagtcg
     7021 aagaaagtaa acaagaactt ttgttgaaat ggatactttt tcttgtaaag aagttctatg
     7081 tgaaaatacc agaaacccat tgtctgatag ttatgcacgg taaaataaca gcttctgcga
     7141 tcgcaaggga aacaaataag ttgctgaata catatgttta tgaggctttc gatatgccaa
     7201 ttgaaggtga gactagtgac ttaattaacc taatcaacaa tttttgtaaa agtatagata
     7261 cgacgaatgg actcattctg ctagttgata tgggatcatt ggaacaaatg tatgaaaaaa
     7321 ttgaatccaa tgtaataggt gatttggtaa ttttaaacaa tgtttctaca gctttagcga
     7381 ttgaatgtgg gattcaagtt tgccaaaaaa agccaattag tcatttttac cagatggatt
     7441 ttgattcgtt tcaagttaaa gttcagtatt ataaaggatt atcacaaaag aaaaatgtaa
     7501 tagtatcttg tctctctggt gaggggattt ctgaaaaagt taaggatatt ttaaaacggt
     7561 atctcgataa tcaagtggag attttgattt ttgattttaa cgcattaaag aaaatagcaa
     7621 aggaaaaaga tactatgatt tttaagaata caatttgtgt tctttcaacc actgaatttt
     7681 acattcaagg aatcgattgt ttaaatttag aaaatctaat taatgggaat caaaccttag
     7741 aaaaattaaa taagtatatg gactcggata gctgtgagtt ctgcttaaat gaattagtta
     7801 aactctttac tattgaaggc gctagtacta agcttcgctt tttagattcc aaaaaagtat
     7861 tcaatgagat agaaaaagta ctatatttgt atgaaaaata ttacgatgtc aaaatcccaa
     7921 gttttctgag aattaatcta tttctacatc tttctggaat gattgaacga attatgattg
     7981 gtgatggaat aacaaatgaa catttgaaag agggagcgca actatttgat acttttttaa
     8041 atgtatcaaa agattttttt tcagaaatca aagatattta ctgtattgaa atacccaaag
     8101 gagagtatga gttaatctat acaatttttg agcagactat tttctaaata gcatactagc
     8161 gtctaaaaaa atgaaagggt gtccaaagta tatttagacg ctcatttaat taatatttac
     8221 tgtattttcc cgctttattg gataatgtta gttggcacag taattgctta tataaaagta
     8281 taagaagaaa ggagagaaca aaatggcgtt ttggcaaaca tttttagttg tgttgtatgg
     8341 atttttcatt aactgggata aggaagggcc caaaatcgga acctcccaac ctgtcgttgc
     8401 aggtttcttg accggactta ttatgggaga tattcaaact ggactttata tcggtgggac
     8461 tttgcaatta atgacactag gtattactag ttttggtggt gcatcagtac cggattatca
     8521 aacagcagca ttgatcggta cttatttagc aatctcaact aaccaggatg cttctatagg
     8581 aataacttta gcaataccgg tagcaatgtt aattgtacaa ctagatgttc taaagtggtc
     8641 aacaaacatt ttttttcaac aaaaggcaga aaaattcgca caaaaagaag attatcaaaa
     8701 attagagtgg atgcaatatt tggcatcaat taatacaagc tttgtatctg gaattcctgt
     8761 tttgttaact gtgatattag gcccaacgtt agtgggaagt gcaatcaatt atattcctga
     8821 atgggttacc ggtggtttaa aggtagcagg gggactatta cctgctgttg ggataggaat
     8881 gctacttaaa tatttaccta caaaagatta ctttgcctat ttgttagttg gtttcgtttt
     8941 ggcagtttat ttccaaatac caattttagg gatttcgtta cttggcgctg caatcgcact
     9001 aatcgtatat aaaaatagat caaataatac tcaagtagca ccagctgcaa caggaggttt
     9061 tgacgaagat gaataagaaa ctgacaaaaa tcgacaaaca agtaattttc cggtggattc
     9121 tcaatggtgc aacttcatta aattatgaaa aaatggaagg gttgggttat gcgtattcta
     9181 tgcttccttt tatcaaggac acatataaag atgatcctga aggaatgaaa caatccgttt
     9241 tagctcacct acaatttttt aacactacgc cgtatacagc gccttacatt attggtatga
     9301 atgttggaat tgaaaaagaa gaaaaaaata agtctttgga agcagtaagt gctctgaaaa
     9361 caggtctaat gggtccttta gcgggaattg gagatagtgt atttgtagtt attccatgga
     9421 ctatttttgg agcaattgca gctaatatgg cattggaagg tagttttgtt ggaattattt
     9481 tgtggatact ggcatcaatt ttattaagga ttactgctta tcctttatac cttgctggga
     9541 aagaatcggg tgcgagccta ctaactcaaa ttgaaacaaa attaaagtct attactgatg
     9601 cagtatcaat tttagggtta atggtggtcg gtgcactcat tgccacagtt gtaaaagtga
     9661 atgttggctt tgaatttaaa caaggcgact taacaatgac cggaaatgaa atctttgatc
     9721 aagttatgcc tggtttatta cctgcatgtg tagtagcaat tgtgtattat ttgttaggac
     9781 gaaaggttaa accagtttat gtaattctat taatcttagt agtttctatt gcgttaagag
     9841 cgttaaatat tttgaagtag gtgttgaata tggatgaaaa aatgaaaggt attatacatg
     9901 ttcgtattga tgatcgaatg attcatggac aagtagcaac acaatggaca aatcaattga
     9961 gtgctaacag aattatggtt attaataatg aagtagccaa tgatgacgta aaaaaagctg
    10021 tagttcgcct cgctgcacca cctaatgtta gaacttcaat aattacaagg gaagtagcag
    10081 ttaaaaatat tctttctggt aaatatgaag gacaaaaagt tcttattgtt gcagtttctc
    10141 caatcgatgt taaatttttg attgataacg gtttaccaat aacaagtgta aacgtaggaa
    10201 atttatcaag aaggaatgga acagaacgca tccgtccgac gataaatatt actgaagaag
    10261 agagagaggc ttttaaagaa ctaattagtg atggagttga agtgacagtg atacaaactc
    10321 ctagagattc aaaagaattt ttaaaggatt ttatttatta agaacagagg atgagtggat
    10381 ggagtttata attacatctc atggaaatat tgccattgga tttgtagata cattaaaaat
    10441 gttttttggc gataatttaa attgtcatgt ggtaacactt ggagatgcag gcattgaaga
    10501 attccgtgaa aacattgaaa aaatagtttc tcaagtaaaa aatcaagaag ttatggtatt
    10561 tgctgacttg attggtggaa cacctttcaa cgaatttgct aaaagaagtg aagtatttca
    10621 aaatgggttt caacttcttg gtggcatgaa tttaggaatt ttgattgagg ctgtcaattt
    10681 gagattacaa ggaaaaaaaa gcgatgatat catcgaaaat ctaagaaata tgaatacaat
    10741 tgcgtgtctt gcagatatgc aaagacatac taacgaagag gatgagtgat aaatgcaaaa
    10801 aattttttac aagcctaaag atttttggtt tggggattgc atgccttttt ataaagatgg
    10861 aactttttat ctatttcatc aacgagatac gagaaatcct aaacctttaa ctgatccttt
    10921 tgggtggtca ctagttacaa ccactgattt tgtgaaattt gaagaatacg gggaagtaat
    10981 tcatcgtggt ggcaacgatg acattgatca atacatttac gctggaagta ttgtccaagg
    11041 aatcaatgag tatttagctt tgtatactgg acataatcgc aaagctaagt tggatggaaa
    11101 aacttcagaa gtgttaatga ttgcaagttc taaagattta attcattgga aaaaggatgg
    11161 cgaagcagca aaacttgttc ctcaagaagg atatgataag aatgattggc gcgatccttt
    11221 tgcaattttt gatcaagatc atggaaaata cgttttgata ctaggagcta gaaaaccagc
    11281 aagtaagaaa aagccgacgg ggagattggt ctattttgaa tccgaagatt taaaagattg
    11341 gaatttcaaa ggagactact gggctaataa tgaatttaac atgattgaaa tgcctgatat
    11401 atttgaaatt gatggaacgt ggtatttact tttttcagag tattgtgaag ataaaaagac
    11461 taaatatcga gtcggagcag gattattttc agattggaaa tcaccagtag acgaagcttt
    11521 cgacggaaaa gcctattatg cagcaagaac agtcggaact aatgaaactg gccgttttct
    11581 aactggctgg gtggcaacaa aagaagggga gaacgatcaa aataactggg attggggcgg
    11641 tgcacttgtt ccacatcaaa ttattcaaaa gacagacaaa actcttggtg ttaaactgcc
    11701 tgaaaaaata attgagaatt ttaaagaaat cactccattt tcaagtttta agttagagaa
    11761 agaatttgga aaaaaagaaa gagtaattgt tcctaaaatc gaagattatt ttctatttga
    11821 aacaactttg actattacat cgttaacaaa ggagtttgct ttaaaattat ttgtagatag
    11881 tgatactcaa gaaggatacg aatttcgtat ttcattgatt aataatcagc ttactttcgg
    11941 aaaaactccg aactatcctt ggcctcaaat gtttgataag gggctgcaac gaccactatt
    12001 tttggaaata ggcaaagaat atgaactaaa gttaattgtt gatgatacga tagctgtttt
    12061 atatattgat aatgttgctt taagtactag aatgtatgat aaagtcggaa cagcattaag
    12121 tatgactgtt acggagggag caatcaaggt agataaacta gactattcaa taaattatga
    12181 atgtggattt taaaacaatt gaagggatga tgtaagtgaa attaaatggt ttacaacata
    12241 ttggaattcc aacaaaagat tttggagctt caaaaatctt ttatgaatcc ttagggttta
    12301 gtctgattaa tgaagaaatg aatgtgggaa gtaaagtcgg ctttttcgag ttgaatggaa
    12361 cgataataga aatatgggaa gataaaacta ctgagtgtag aggagccatt gatcatctag
    12421 cattagatac ggatgacata gatgaggtat tcaattcaat tcaagattta ggatatgaat
    12481 tattagataa aaatattatg gaattaccgt tctgggaaaa tggaatccgt tactttaatt
    12541 tctacggacc aaatcatgag attatagaga taagccaaat gaagaaataa atagataacg
    12601 aagtgctcta agttggtatg aacgaaaagt acttcatttt ttggtatcag catttcaata
    12661 gtttaatgag aatttactat ttcaaattaa ttcaaagtga cagtattttt tacttatttg
    12721 gtttcaaatt ttgtaatcct tcctatttat tggaatacac tccacatttt gtatacaagt
    12781 catatcatgt tttagcaaag ctcttaccaa gcaaagttat aatgatgttt tccaaagttt
    12841 gatctttcga tggaggctaa cacatatttt ttgcaggaca gccaaaatta gatgtataga
    12901 atagagtctg tgaggcgtat agacagttta ttgtctgtat gccttttttt attttgaaaa
    12961 atttttagta tttttcgaaa aatgctcact cctagagtaa gcgccccata gaacagtacc
    13021 ttactaacaa atgcacctca agatatactt gagctatcaa gaagtctgga ggttttct
//
LOCUS       DACCVU010000081        12899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCVU010000081 DACCVU010000000
VERSION     DACCVU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.081"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..452)
                     /locus_tag="IVW29_002015"
     CDS             complement(90..452)
                     /locus_tag="IVW29_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7839494.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(463..1584)
                     /locus_tag="IVW29_002016"
     CDS             complement(463..1584)
                     /locus_tag="IVW29_002016"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7839495.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1600..1950)
                     /locus_tag="IVW29_002017"
     CDS             complement(1600..1950)
                     /locus_tag="IVW29_002017"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7839496.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2080..3591)
                     /locus_tag="IVW29_002018"
     CDS             complement(2080..3591)
                     /locus_tag="IVW29_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7839497.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3913..5265)
                     /locus_tag="IVW29_002019"
     CDS             complement(3913..5265)
                     /locus_tag="IVW29_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7839498.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5418..6494)
                     /locus_tag="IVW29_002020"
     CDS             complement(5418..6494)
                     /locus_tag="IVW29_002020"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7839499.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6690..8435
                     /locus_tag="IVW29_002021"
     CDS             6690..8435
                     /locus_tag="IVW29_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7839500.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8715..9152)
                     /locus_tag="IVW29_002022"
     CDS             complement(8715..9152)
                     /locus_tag="IVW29_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7839501.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9136..10509)
                     /locus_tag="IVW29_002023"
     CDS             complement(9136..10509)
                     /locus_tag="IVW29_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839502.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10831..11319)
                     /locus_tag="IVW29_002024"
     CDS             complement(10831..11319)
                     /locus_tag="IVW29_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839503.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11341..11943)
                     /locus_tag="IVW29_002025"
     CDS             complement(11341..11943)
                     /locus_tag="IVW29_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7839504.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(12012..>12899)
                     /locus_tag="IVW29_002026"
     CDS             complement(12012..>12899)
                     /locus_tag="IVW29_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7839505.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         3694 a         2755 c         2283 g         4167 t
ORIGIN      
        1 aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat
       61 gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata
      121 cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag
      181 accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag
      241 tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta
      301 gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta
      361 ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga
      421 tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct
      481 tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc
      541 tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc
      601 gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt
      661 ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc
      721 tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc
      781 atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc
      841 aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta
      901 cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc
      961 actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa
     1021 gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt
     1081 aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac
     1141 ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga
     1201 aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg
     1261 tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac
     1321 catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc
     1381 taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat
     1441 agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag
     1501 tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt
     1561 tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg
     1621 tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga
     1681 acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac
     1741 ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc
     1801 taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt
     1861 caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc
     1921 gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac
     1981 actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat
     2041 gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat
     2101 tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt
     2161 tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg
     2221 gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg
     2281 gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag
     2341 caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag
     2401 gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa
     2461 ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt
     2521 caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat
     2581 accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg
     2641 agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac
     2701 gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg
     2761 tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc
     2821 gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt
     2881 ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata
     2941 gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt
     3001 tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa
     3061 tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat
     3121 attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt
     3181 gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc
     3241 tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac
     3301 ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt
     3361 tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg
     3421 taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc
     3481 agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg
     3541 ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct
     3601 cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca
     3661 aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt
     3721 ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt
     3781 tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt
     3841 ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa
     3901 gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct
     3961 gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat
     4021 caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa
     4081 ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg
     4141 atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat
     4201 atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac
     4261 cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc
     4321 tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat
     4381 ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac
     4441 attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg
     4501 tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt
     4561 gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac
     4621 gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc
     4681 tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg
     4741 ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac
     4801 gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt
     4861 gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc
     4921 tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc
     4981 caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg
     5041 aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt
     5101 gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact
     5161 tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca
     5221 ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa
     5281 aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa
     5341 gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc
     5401 ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga
     5461 ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc
     5521 cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg
     5581 aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac
     5641 atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg
     5701 gctggaatct gcatttcgat tttattattg atatattttg cttcataatc atagaatgct
     5761 acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca
     5821 acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag
     5881 gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta
     5941 gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca
     6001 aatacttttt taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc
     6061 ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg
     6121 gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt
     6181 ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt
     6241 tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat
     6301 gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat
     6361 tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt
     6421 aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt
     6481 aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat
     6541 attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct
     6601 aaaagaacga gcgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa
     6661 agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg
     6721 cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa
     6781 atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac
     6841 agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat
     6901 tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac
     6961 tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt
     7021 ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc
     7081 aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc
     7141 ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag
     7201 taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat
     7261 tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt
     7321 gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga
     7381 acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag
     7441 aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc
     7501 atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac
     7561 aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag
     7621 cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc gattttggta
     7681 tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt
     7741 atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct
     7801 cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt
     7861 ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc
     7921 aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag
     7981 aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg
     8041 ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa agcggattag
     8101 acttattgga agggatgaag gctgcgccta agatccctca acagctccac catctattag
     8161 aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat
     8221 tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga
     8281 tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct
     8341 tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg
     8401 cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca
     8461 gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg
     8521 gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt
     8581 tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc
     8641 ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct
     8701 gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct
     8761 ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta
     8821 tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga
     8881 tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc
     8941 aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg
     9001 tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg
     9061 gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact
     9121 ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata
     9181 aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag
     9241 ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg
     9301 ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac
     9361 actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt
     9421 atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag
     9481 cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag
     9541 tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac
     9601 tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc
     9661 taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat
     9721 tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg
     9781 tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa
     9841 aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat
     9901 caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag
     9961 ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat
    10021 agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa
    10081 tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt
    10141 ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa
    10201 aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc
    10261 agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag
    10321 actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat
    10381 cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac
    10441 cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt
    10501 ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc
    10561 attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag
    10621 aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc
    10681 tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt
    10741 atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg
    10801 cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc
    10861 gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc
    10921 atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct
    10981 ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg
    11041 accacgtact ttatttagca aatataattt acttaaataa atttttccat ttatctcttc
    11101 ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc
    11161 tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg
    11221 tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga ctgccatcag
    11281 ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca
    11341 ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac
    11401 cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa
    11461 accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag
    11521 tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt
    11581 tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat
    11641 tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat
    11701 cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac
    11761 ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa
    11821 agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa
    11881 aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga
    11941 catctatatt ccccacatct tttttcattt attaaaactg cctccttatg aactgaaccc
    12001 caaaagttga actatttaat ggactgtttc cgatattcta ctggagatag tccatttaac
    12061 tttagtttta tccttttgtt attataccac ctaatatact catttaattt actttgaaat
    12121 atctcaattg atcggaattt ttctcgataa aaaaactctg atttaagcac accaaaaaaa
    12181 ttttctatta cagaattatc taagcaattt ccttttctag acatgctttg aataatgtta
    12241 ttctctttta attttttttg atattgtggc atctgatact gccatccttg atctgaatgt
    12301 agaatcagtg aaccctcagt tccctttttt ttaattgctt gttgaagcat ttcttcaatc
    12361 agtttatatg ttggactagt tgatatacta taactaatta tttctccgtt aaataaatca
    12421 agtataggag atagatagat ttttcttcct tttatcttga attcagtgac gtctgtcacc
    12481 cattttttat tgggtgtatc tactgaaaaa tttcgtttta acacattctt ggcaattttt
    12541 cctactgttc ctttataaga tttatatcgc tttattcgga tttgacaggt aagtcccatt
    12601 tgggacatta attttctaac tgttttatga ttgattgtat atcctttcat ctttaacgct
    12661 aaagtgactc tacgataacc ataagagttt cttgattctt ttacaatcgc tgtaatttct
    12721 tgctttatct tgctatattt atctggctta tctaattttt ttacccagta ataataagtt
    12781 gacttcgcta attgtgcaat tgaaagtaat agattcaatt taaattcttt tttgagctga
    12841 aggattgttt taaccttgat ttcttcttgc tcaaatcttg ttcttgaatc aaggtttct
//
LOCUS       DACCVU010000082        12862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCVU010000082 DACCVU010000000
VERSION     DACCVU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.082"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            127..3654
                     /locus_tag="IVW29_002027"
     CDS             127..3654
                     /locus_tag="IVW29_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7839506.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IVW29_002028"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IVW29_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7839507.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IVW29_002029"
     CDS             7442..7783
                     /locus_tag="IVW29_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839508.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IVW29_002030"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IVW29_002030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7839509.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IVW29_002031"
     CDS             9140..11560
                     /locus_tag="IVW29_002031"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7839510.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IVW29_002032"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IVW29_002032"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7839511.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4365 a         2240 c         2729 g         3528 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta ga
//
LOCUS       DACCVU010000083        12831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCVU010000083 DACCVU010000000
VERSION     DACCVU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.083"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..2107
                     /gene="acm"
                     /locus_tag="IVW29_002033"
     CDS             <1..2107
                     /gene="acm"
                     /locus_tag="IVW29_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7839512.1"
                     /translation="LIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKK
                     QNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNL
                     DSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGD
                     MLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRG
                     EEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENH
                     SEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFE
                     LRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKF
                     SINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAE
                     TKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNEN
                     KEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKY
                     RDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTERE
                     KIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVII
                     GLLIVTICAGIFLQKYKKV"
     gene            complement(2379..2864)
                     /locus_tag="IVW29_002034"
     CDS             complement(2379..2864)
                     /locus_tag="IVW29_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7839513.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3344..3985
                     /locus_tag="IVW29_002035"
     CDS             3344..3985
                     /locus_tag="IVW29_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7839514.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4004..4639
                     /locus_tag="IVW29_002036"
     CDS             4004..4639
                     /locus_tag="IVW29_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7839515.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4657..5472
                     /locus_tag="IVW29_002037"
     CDS             4657..5472
                     /locus_tag="IVW29_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7839516.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5791..7227
                     /locus_tag="IVW29_002038"
     CDS             5791..7227
                     /locus_tag="IVW29_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7839517.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7416..9863
                     /locus_tag="IVW29_002039"
     CDS             7416..9863
                     /locus_tag="IVW29_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7839518.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10093..11160
                     /gene="rlmN"
                     /locus_tag="IVW29_002040"
     CDS             10093..11160
                     /gene="rlmN"
                     /locus_tag="IVW29_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7839519.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11353..12456)
                     /locus_tag="IVW29_002041"
     CDS             complement(11353..12456)
                     /locus_tag="IVW29_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7839520.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4378 a         2165 c         2719 g         3569 t
ORIGIN      
        1 actgattcct gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc
       61 gttgaccgtg gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt
      121 tgacgaaaaa aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga
      181 aggaaatatt cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa
      241 tgttggaact ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa
      301 aaacctagac tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga
      361 taccggtgaa gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt
      421 aaatgtgaca aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga
      481 catgctacct gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac
      541 ttatgttgaa caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc
      601 aagtacgttt gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga
      661 gggaatccaa caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga
      721 taattacatt gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag
      781 ttataagacg atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg
      841 gtttaaagaa tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa
      901 tatttcagct tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata
      961 tatcaatgac acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga
     1021 acagcctatt caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag
     1081 tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat
     1141 tgattttgat ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga
     1201 gggagtcagt ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat
     1261 ttggaacggt ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac
     1321 aaacaattgg gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc
     1381 agtgacttgg gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt
     1441 gcaagaagta gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa
     1501 cgggctagtc gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg
     1561 ggaagacaat gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc
     1621 cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt
     1681 cacaaatctg ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa
     1741 cgtgcccgaa tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga
     1801 aaaaacaaat atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa
     1861 gacagagaga gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac
     1921 aggagaatcc agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat
     1981 aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt
     2041 aatcattggt ttattgatag taactatctg tgctgggata ttcttacaaa aatataaaaa
     2101 agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc
     2161 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
     2221 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
     2281 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
     2341 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
     2401 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
     2461 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
     2521 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
     2581 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
     2641 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
     2701 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
     2761 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
     2821 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
     2881 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
     2941 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
     3001 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
     3061 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
     3121 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     3181 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     3241 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     3301 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     3361 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     3421 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     3481 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     3541 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     3601 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     3661 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     3721 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     3781 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     3841 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     3901 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     3961 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     4021 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     4081 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     4141 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     4201 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     4261 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     4321 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     4381 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     4441 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     4501 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     4561 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     4621 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     4681 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     4741 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     4801 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     4861 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     4921 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     4981 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     5041 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     5101 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     5161 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     5221 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     5281 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     5341 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     5401 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     5461 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     5521 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     5581 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     5641 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     5701 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     5761 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     5821 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     5881 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     5941 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     6001 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     6061 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     6121 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     6181 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     6241 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     6301 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     6361 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     6421 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     6481 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     6541 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     6601 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     6661 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     6721 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     6781 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     6841 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     6901 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     6961 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     7021 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     7081 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     7141 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     7201 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     7261 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     7321 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     7381 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     7441 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     7501 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     7561 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     7621 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     7681 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     7741 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     7801 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     7861 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     7921 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     7981 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     8041 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     8101 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     8161 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     8221 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     8281 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     8341 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     8401 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     8461 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     8521 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     8581 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     8641 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     8701 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     8761 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     8821 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     8881 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     8941 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     9001 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     9061 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     9121 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     9181 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     9241 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     9301 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     9361 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     9421 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     9481 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     9541 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     9601 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     9661 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     9721 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     9781 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     9841 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     9901 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     9961 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
    10021 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
    10081 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
    10141 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
    10201 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
    10261 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
    10321 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
    10381 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
    10441 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
    10501 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
    10561 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
    10621 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
    10681 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
    10741 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
    10801 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
    10861 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
    10921 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
    10981 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
    11041 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
    11101 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
    11161 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
    11221 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
    11281 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
    11341 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
    11401 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
    11461 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
    11521 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
    11581 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
    11641 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
    11701 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
    11761 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
    11821 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
    11881 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
    11941 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
    12001 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
    12061 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
    12121 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    12181 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    12241 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    12301 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    12361 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    12421 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    12481 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    12541 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    12601 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    12661 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
    12721 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
    12781 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga t
//
LOCUS       DACCVU010000084        12741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCVU010000084 DACCVU010000000
VERSION     DACCVU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.084"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(39..848)
                     /locus_tag="IVW29_002042"
     CDS             complement(39..848)
                     /locus_tag="IVW29_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7839521.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="IVW29_002043"
     CDS             1141..2577
                     /locus_tag="IVW29_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7839522.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2747..3943
                     /locus_tag="IVW29_002044"
     CDS             2747..3943
                     /locus_tag="IVW29_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7839523.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4005..4190)
                     /locus_tag="IVW29_002045"
     CDS             complement(4005..4190)
                     /locus_tag="IVW29_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7839524.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4421..7741
                     /gene="dnaE"
                     /locus_tag="IVW29_002046"
     CDS             4421..7741
                     /gene="dnaE"
                     /locus_tag="IVW29_002046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7839525.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            7908..8870
                     /gene="pfkA"
                     /locus_tag="IVW29_002047"
     CDS             7908..8870
                     /gene="pfkA"
                     /locus_tag="IVW29_002047"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7839526.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8944..10728
                     /gene="pyk"
                     /locus_tag="IVW29_002048"
     CDS             8944..10728
                     /gene="pyk"
                     /locus_tag="IVW29_002048"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7839527.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10763..11665)
                     /locus_tag="IVW29_002049"
     CDS             complement(10763..11665)
                     /locus_tag="IVW29_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7839528.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11851..12420
                     /locus_tag="IVW29_002050"
     CDS             11851..12420
                     /locus_tag="IVW29_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7839529.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12478..12657
                     /gene="rpmF"
                     /locus_tag="IVW29_002051"
     CDS             12478..12657
                     /gene="rpmF"
                     /locus_tag="IVW29_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7839530.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
BASE COUNT         4013 a         2250 c         2675 g         3803 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatctctttt tgttttacat aaaaagaacg
     2641 ttacatataa aatgaccttt gcgggatttt tctcttttta ctggcgagat cgttgcttgt
     2701 ttattcgaaa ataggtatga taaaagagac gaaagaggga tgatagatgg agattaattg
     2761 gagaaaaaat ttagtgattg cgtggattgg ctgttttttc actggtgcaa gtattagttt
     2821 agttatgcca ttcataccag tttatgttga gcaactagga acaccaaaag atcaaatcga
     2881 attgttttcg ggactagcaa tctctgtgac tgcttttgca tctgccgtgg ttgcaccaat
     2941 ctggggaaat ctagctgatc gaaaaggccg aaaactcatg atgatccgtg cagcagcagg
     3001 gatgacatta acaatgggat ctttggcttt tgtaccaaac gcgtattggc ttttgatcat
     3061 gcggttttgg aatgggattt tgtctggata tattccaaat gccacagcga tgattgcttc
     3121 acaggcacct agagaaaaga gcggatgggc gttaggaaca ttgtctaccg gagcgattgc
     3181 tggaaatctg atcggacctt caatgggcgg tgcactggct cagtggtttg gtatggaaaa
     3241 tgtattcttg atcacagggg ctttgttgat gatcacaacg gtattgacca ttttcttagt
     3301 caaagaagat ttccatccaa tcgagaaaaa agatctgata agcaccaaag aaattttttc
     3361 aaaaatggat catctctcta ttttgatcgg tctcttcatt acgaccttga ttttacagat
     3421 aggaatcact acgatcagcc ctattttgac cttatatatt cgtgaattga gcggaagtac
     3481 agaaaatatc ctttttgtca gcggcttgat tgtttcgata gctggtgttt ccgctgtctt
     3541 ttcttcaccg aaactaggaa aactagggga taagattggg aatcaaaaag ttctaattgg
     3601 aggactcatt ttatcatttt gctgttattt acctatggca ttcgtcacta caccgctcca
     3661 attaggtata cttcgcttta ttttaggctt ttcaactggc gctttgatgc cttctgtcaa
     3721 tacactgatc agtaagatta cgcctcaaga aggagtcagc cgaatctata gctacaatca
     3781 gatgtttagc aactttggac aagttttagg tcccatgcta ggatcgacag tcgcgcatgc
     3841 ttataattac agtgctgttt ttattgtgac aagtttgttt gttctgtcaa atattcttct
     3901 ttctttattc aactttcgta aagttcttcg cagcagatta taatggtggt gaggagtaga
     3961 aaataaaaaa gagcttgaga aatctcaagc tcttggctgt ttttttacga atgggcagag
     4021 gtttcttttc ttggcgcaac tgcaccaatc aaaaggataa tgacacccgc aatgaatgaa
     4081 atgattgttg tcaaagtaaa atcatatgtt ccgcttgtta atgcagcacc aatataaccg
     4141 actacttgac ctagaacgac agcccagaat aatgtaacga tatatcgcat aaagaacacc
     4201 tcttttctat cttaatttta ccagattttc tttgaatgta aagaaacgag accaaatctc
     4261 aataaaattt gagttcttgc gaaagaactc attcctacag attacaagca agcaaatgaa
     4321 gtggaataat caacttcagc gcctgctatc ttaatgaaag taaaaggatc aaacctttta
     4381 acgctagtga attttaacga aaaaaaaggc ggaatgcaag atgtttttcc cacaacttta
     4441 tacaattact tcttattctc ttttacaaag tacgatacgt attcgagaat atgtacaaga
     4501 agcaaaaaaa caaggctaca cttcattagc cataacggat aaagatgtct tatacggtgc
     4561 acctgaattc tatcgtattt gtgtagaaga aggagtacag ccaatcattg gattacatct
     4621 tagttatact tatgaagaaa aaaattatgg cttgcttgcg tacgcaaaag atgaaatagg
     4681 ttatcagcag ctgatgaaac tttccagccg aaaaatgatc caagaaaaat tggaacttgc
     4741 agaaatgaca gaaaccgacc atctagcgtt tgttgttccg gcaaatcatt ctgtttctga
     4801 ggctttcaat aaagaggata actttttcgg tcattggcag tttttacttg aaaatgtttt
     4861 tactagaaat ttttatatcg gcttgacaga aaacacgcct gatcaattgc ctgaatggta
     4921 tcagtatgtg ataaaagaaa attattcgct ttgtgcatta catcccatcg ctagtttaca
     4981 acaagaagaa ctttttgcac ttgaagtgat gaaacatatc aaagagggaa gccaaatacc
     5041 tttagaaaat ttatctgcat ctttaccact aacagcaaat gtctatcttc tttcagaaca
     5101 agaaatgatc aaacgattcg aaaaagcggg tctttcccaa gctgtaatag gcgcccagcg
     5161 tttagctgaa tcgatttcat ttgaattcca gttccatcag aaattacttc cgcattatcc
     5221 agttcctaat ggaaaacaag ctggagaata tttaagagag ttatgctatt cgcaagtgga
     5281 acgccgaatc actgactata caaaggaata ccaagaacgt ttggactacg agttatctgt
     5341 catccatcaa atgggattcg atgattattt cttgatcgta tgggatgtaa tggcttttgc
     5401 tcatagaaac aaaatcgtta ccggtgcagg tcgtggttct gctgcgggtt cattagtagc
     5461 ctatgtgtta gaaatcacag atgtggatcc attagaatat gatttgctgt tcgaacgctt
     5521 tttaaacccc gaacgcttta cgatgccaga tatcgatttg gatattcctg acaatcgacg
     5581 tgaagaagta ttgaattatg ttcgagaaaa gtatggacaa tatcatatgg cacaaatcgc
     5641 aacatttggc acaatggcag caaaaatggt tcttagagat gcagctagag tgtttggttt
     5701 atcgcaaagt gaagcaaacc gctggtcaaa agcaataccc aatcaattga aaatcacatt
     5761 gacccaagct tataaaactt cgcatcaatt agtagaatta gttcaggcaa gcgaaaaaaa
     5821 ccaactttta ttcaaaactg cagtgatttt agaaggattg ccaagacatg tttcgactca
     5881 cgcagcaggt gtagttatca gcgataagaa tctacttgaa ctagttccgc tccaacgcgg
     5941 ttctgatgaa gcttttctga cccagtatac gatgaatgag gtagaagcaa tcggtctttt
     6001 gaaaatggac tttttaggac tacgcaactt atcgattatt gattttactt tgaaagctgt
     6061 taagcgagta gaagggtatg agatccgatt aaaagagatc ccgctaaatg atgcaaaaac
     6121 gctgctcttg tttcaacgag gcgaaacctc aggagtattt caatttgaat ctgctggtat
     6181 ccggaacgtt ttacgaagat tagatccaga aaacatcgaa gatattgccg cagtcaatgc
     6241 attgtatcga ccaggcccta tgcaaaatat agataccttc attgccagga agaaaggaaa
     6301 agaagccatt cgctacccag atgattcact gatcccgatt ttgaaaaaca catatggagt
     6361 gatcgtctat caggaacaaa tcatgcagat cgcctcgact atggctggat ttacattagg
     6421 gcagtcagat attctgcgac gggcagtgag taaaaagaaa aaagacgtat tagatgaaga
     6481 aaagtcgcat tttgtatcag gagcattaca acagggatat tcggaaaaaa cagcagcgac
     6541 cgtatatgaa tatatcgaaa aatttgcaaa ctatggattc aatcggtcac atgcatttgc
     6601 ttattcattc gtgggattcc aaatggcgta tctaaaagcc catcatccag gtgctttctt
     6661 taccagcctg atgaattctg ttcggcataa cacatcaaaa ttgaaagaat atattgcaga
     6721 agcacgtaaa aataaactca aactttcctc gccgtccatt aatcaaagta attccagttt
     6781 tgagttggtc aataaggaag aaatcagatt cggactgaca gcgataaaag gtattcggcg
     6841 tgattttgta gaagatatat taaacgaaag aaaacaaaat ggcccgttcc agtcagtgga
     6901 tcaattcctc atacggatcg ataagcgttg gctgaaatta gagttactgc aatcactgac
     6961 agcagtagga gtttttgatg aattatctcc taacaggaaa caattgatgc ttgatctcga
     7021 aggaaaaatc caaaatatcg tttacagtgg tggaagtctt gacttgctgg gaatcatggc
     7081 attaaaagaa gaagaagtcg ctgactatac actagaagaa cgcttgaagc tggaagagga
     7141 gtacttaggt atttatttgt caggtcatcc tgcagaaggg tttgatcgtc ttcgtttagc
     7201 taaacaagtt cagctgatta cagatatcgt ccctaatcaa ccagtcaatt tattagtgat
     7261 gatcaaggaa gttagagaaa ttcggacaaa aaaaggagaa ctgatggcct ttgttgaagg
     7321 aaccgacagt agtggagaaa tttctgtcac agtttttcca actctttatc gtaaatatcg
     7381 taatttgttt gccgttgatc gagtgatctt tgttcaaggg cggagcgaaa tcagcaaata
     7441 taatcaggaa ctgcaactaa ttggagaaag ctttgctgat cctaaccagt tagaagccca
     7501 gtatccttca caaacttgct atttgcgtat tacagaagaa cgggaagatc ccgagataat
     7561 gaaacaatta caagtgattt ttcagaaaca cccaggatat ataccagtag ttatgtatca
     7621 cgaaaaaacg caacgaaaag tagttctgtc agatgcttat tgggtggatg acagcaagag
     7681 taagagtata aaggggcaac ttatttatct gcttggcgaa gataatgtgg ttttcaaata
     7741 acttacggta aaaaatacta cttgctttca tttttttcat ttttttatcc gtaaatcaag
     7801 acaagtgaga aaaataatgt tagaatgtta gtcgtggtta acgcaaaaat ggttgtaaac
     7861 ctaagtcata cttaggataa agtattttaa tgaggtgaat gtttattatg aaacgcatcg
     7921 gaattttgac cagtggcgga gacgcacctg gaatgaatgc agctgttcgt gccgttgttc
     7981 gtaaaggtat ttacgaagga ctggaagtat acgggatcaa ttatggtttt gccggtttag
     8041 ttgctggaga tattcgtcgt cttgatgtgg cagatgtagg tgacaagatc caacgtggtg
     8101 gtacattctt gtattctgca cgttatccag aatttgcaac tgaagaaggt caattaaaag
     8161 gaatcgaaca attgaaaaaa ttcggaatcg aaggtctagt ggttatcgga ggagacggct
     8221 cttatcatgg cgcaatggct ttgacaaaac gcggtttccc agcagtaggt attcctggaa
     8281 caatcgataa tgatattcct gggactgatt tcacaatcgg ttttgataca gcaatcaata
     8341 cagtactaga atcaattgac cgtattcgtg acacagcgac ttctcatgtt cgtacattcg
     8401 ttatcgaagt aatgggacgc aatgcaggag acatcgcttt atggtcaggt gtggctggcg
     8461 gagcggatga aatcatcatc ccagaacatg attttgatat ggcttcagtt gctaaaaaaa
     8521 tccaagaagg ccgcgatcgc gggaagaaac attgcttgat catcttggct gaaggtgtaa
     8581 tgggcggaaa tgaattcgct gagaaacttt cagaatttgg tgactatcat acacgtgtat
     8641 ctatcttggg acacgttgtc cgtggtggag caccaagcgc acgcgaccgc gtgttggcaa
     8701 gtaaattcgg tgcttacgct gtcgaattgc ttcaagaagg aaaaggtgga ttatgtgtcg
     8761 gtatccatga caacgaaatc gttgcgtctg acatcattga tacacttgaa aacaacaaac
     8821 ataaaccaga cttatcacta tatgatctaa ataattcact ttcattttaa gagtgaaaga
     8881 aaccaagcta tttaagctgt gtacagcttt aaataaacaa aattgaatgg gagagttttt
     8941 taaatgaaaa aaaccaaaat cgttagtaca ttaggaccag caagtaactc agtagaaatt
     9001 atttctcaat tgattgaatc tggggcaaat gtcttccgct ttaatttttc tcatggtgac
     9061 catgaagaac aattatcacg tatgactatg gtccgtgaag cagttaaaaa gacaggaaaa
     9121 gatgtcggta ttcttttaga tacaaaagga gctgaaatcc gtacaactgt tcaaggcaca
     9181 acagaagctg attttggtcg tgcaggatac atcaaatttg aagtaggaga tcaaacacgc
     9241 atctctatgg atcctgaaca tgtaggttca aaagaaaaaa tcgcggttac ttacccagga
     9301 ttatttgacg atgttcacgt tggcggacac attttatttg atgatggttt gatcgatatg
     9361 caaatcattg aaaaagacga agcaaaccgt gaattagtcg tagaagttaa aaatgcaggt
     9421 atgctaggat cacgtaaagg ggtaaatgca ccaggcgttt ctattagttt gccaggtatt
     9481 actccaaaag atgctgacga tattcgtttt ggtttagaca acgatatcga ctttatcgct
     9541 gcaagttttg tacgtaaagc acaagacgtt ctagatatcc gcgaaatctt agaagaaaaa
     9601 gacatgactc atgttcaaat cttccctaaa atcgaatctc aagaaggtat cgacaacatt
     9661 gacgaaatca tcaaagtttc tgacggtgtt atgattgccc gcggagatat gggtgttgaa
     9721 atcccagctg aactagtacc aatggttcaa aaacgcatta tcaaaaaatg taatgctgct
     9781 ggtatcccag ttatcactgc tacacaaatg ttagaatcaa tgcaagaaaa cccacgtcca
     9841 acacgtgcgg aagcttctga cgttgctaac gctgtatttg atggcacaga tgcaacaatg
     9901 ctatctggtg aatctgcaaa cggggactac ccagttgaag ctgtttcaac aatggcacgt
     9961 atcgaccaag aagctgaaaa tgcattatca gaattaggta cattccaatt gaacgaattc
    10021 gataaaacag atgtaactga aacaatcggt ttatctgtag ctcgtgctgc aaaaaatcta
    10081 ggcgtgaaaa caatcgttgc tgcaactgaa tcaggataca ctgcaaaaat gatttcaaaa
    10141 tatcgtccag atgcagatat cttggctgtt acttttgacg aacgcacaaa acgtggtttg
    10201 atgttaaact ggggcgttta cccaacagtt gctgaaaaac catcaacaac tgatgaaatg
    10261 tttgaactag cagctaaaaa agctgttgaa ttaggtttcg catcagaagg cgatttaatc
    10321 ttgatcactg ctggtgtacc agttggtgaa cgcggaacaa ctaacgtgat gaaaattcaa
    10381 atgattggtt ctaaattatt agaagctcaa ggcgttggac aaaaatctgt cgttgcaaat
    10441 gcagttattg cttcatctgc tgaagaagct gtaaaaaaag caaaagacgg aatggttcta
    10501 gttgtaccat caactgacaa agaatatatg cctgcaatcg aaaaagcagc tgctgttatc
    10561 gttgaagaag gcggattgac ttctcacgct gcagttgtag gaattgcaca agatattcct
    10621 gtaatcgttg gtgctaaagg cgcaacaaca acaattacag aaggtgaatt gattaccgtt
    10681 gattcacgtc gcggcatcgt ttaccgtggc gaaacaacag ctatctaatt tagcttaata
    10741 aaaaataaaa acagctcttc ttttacgaag agctgttttt ttgtgctgta acgaattcat
    10801 ggataacttg gactacttgt tcttgaagag gcgtgacgat ctgctgtgtt cgttttgcca
    10861 atgaaatcaa gaagcgaggc tgaacttcat ctgaaagagg gaggcaaact aaatgatctt
    10921 caggatggac agcaatctct gccaaaaaac cgatacccac attttctcgg accatactct
    10981 ttaaaatcgt caagtcattg ctttgataaa caacttttgg atgagtatgt gtttgtttag
    11041 aaagcttagt gaatgctgcg gggtggacat aatgttcgtt caatagtaca aactgttcat
    11101 ctgctatatc tttgaaagag acagtatcct gcttggaaag aggatgtgta ggtggcacga
    11161 cgatcatgaa acgtttgtcc aaaagtaact ctgtttctaa atattgatcc acgattggct
    11221 gtgttgagcc taatacagct aaatcgatcc tcccttgtcg gatcatcccg tataagtccc
    11281 gagagcctcc atcggtaatc tcaatttgat tcatcaattc atgcttgata aagtaacttg
    11341 acagtttagg aaagtattca ttgccgataa taggaggcat gccaaaacga agctttttct
    11401 tttgcaactg agtgatttca gctcttgcag tagaaatctc ttggagaatg gtttgtgcat
    11461 ggagaaacag ctgctttcca gaaggcgtga cgattaattc gtgatgcgat cgatctcttg
    11521 taataagtac ggtgtcaaac tctttttcaa gacgttgtaa tgcatatgta atagtaggtt
    11581 ggctcacacg gaaataatta gccactttcg taaaattttt ttgtttgacg atttggacat
    11641 agtattcgat atctttcgtg ttcatgaaga tcatccgctc ctttaatgga ataatgatta
    11701 ttgtaaatga ttctgaggat gatagcaaac agaggctttc taataaagat ttttctgttt
    11761 ttttatgaac tgcaaggtgt caagaaaatt tcattgacaa catgcttttt gccctgtata
    11821 attgcttttg ttgcttagga gtgatgaaaa atgaaatggt ctttattgga attgagaaaa
    11881 taccaggaga caccgttaac ttttcatgag acgctcgact tgaaagccgc tctcatgaag
    11941 cgagacaatt taatacttga tgttgcgcca gtggaagtcg aaggtttggt ctcagttgat
    12001 aagaaaggct atatcgttca ttacactgtg cagacgactt tgacagttcc ttcaacacgt
    12061 tcactagaac ctgttgaact gcccatggat ttttctgtag acgaagtatt catgacagaa
    12121 gaacaattcc aaagtagaga tgaacaaatg tctgcagatg agattttgct aattgaaggt
    12181 ccattattga atttagcaga gtcgattgaa gacaacatct tattggcgat tccaatgcgg
    12241 gtattgacaa aagaagagga agcagcaaca gaacttccaa aaggcaatga ttgggaagtt
    12301 gtttccgaag aagaatacca gcgcagaaaa atggaagaag ccgagaataa aattgatcct
    12361 cgtcttgcaa aactatctga actttttgat tctaaatcaa aagacgacga taacaagtaa
    12421 gattggaata tattgtgtct aatttagata caagacttta caaggaggtg tagcaaaatg
    12481 gcagtaccag ctagaagaac atcaaaagct aaaaaagcaa aacgtcgtac acactacaaa
    12541 ttgacaatca aagggttaaa cgaatgtcca aactgtggtg aaatgaaaaa aagccatcac
    12601 gtatgtgcaa attgtggtta ctatgatggt aaggacgtaa tgtcaaaaga agcttaatac
    12661 attttaatta gaaaatgtaa aacctcaagg ctaaccatgg agccttgagg tttatttttt
    12721 tgtagtcggt aaataaaaaa t
//
LOCUS       DACCVU010000085        12557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCVU010000085 DACCVU010000000
VERSION     DACCVU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.085"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..610
                     /locus_tag="IVW29_002052"
     CDS             5..610
                     /locus_tag="IVW29_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7839531.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            630..992
                     /locus_tag="IVW29_002053"
     CDS             630..992
                     /locus_tag="IVW29_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839532.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            995..1177
                     /locus_tag="IVW29_002054"
     CDS             995..1177
                     /locus_tag="IVW29_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839533.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1194..4613
                     /locus_tag="IVW29_002055"
     CDS             1194..4613
                     /locus_tag="IVW29_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7839534.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4664..5401
                     /locus_tag="IVW29_002056"
     CDS             4664..5401
                     /locus_tag="IVW29_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7839535.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5411..7702
                     /locus_tag="IVW29_002057"
     CDS             5411..7702
                     /locus_tag="IVW29_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839536.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7726..9816
                     /locus_tag="IVW29_002058"
     CDS             7726..9816
                     /locus_tag="IVW29_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839537.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            9979..10425
                     /locus_tag="IVW29_002059"
     CDS             9979..10425
                     /locus_tag="IVW29_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839538.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            10427..10564
                     /locus_tag="IVW29_002060"
     CDS             10427..10564
                     /locus_tag="IVW29_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7839539.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            10602..10895
                     /locus_tag="IVW29_002061"
     CDS             10602..10895
                     /locus_tag="IVW29_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839540.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            10892..11116
                     /locus_tag="IVW29_002062"
     CDS             10892..11116
                     /locus_tag="IVW29_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7839541.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            11113..12138
                     /locus_tag="IVW29_002063"
     CDS             11113..12138
                     /locus_tag="IVW29_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7839542.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            12328..12401
                     /locus_tag="IVW29_002064"
     tRNA            12328..12401
                     /locus_tag="IVW29_002064"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12362..12364,aa:Ile,seq:cat)
BASE COUNT         4382 a         1956 c         2644 g         3575 t
ORIGIN      
        1 aacaatggct aaaacaacaa cagtaacaac ttttgacaat ataagtatta aaagaatttc
       61 ttttaatttt aagaatgcaa caaatgcaat ttcaactgat tgtaatggac aattagatgg
      121 cgaaacagaa atgcagagaa ttgtaaaaaa atgcggttca acagaagtaa aatcgaaatc
      181 taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag tttatcgacg
      241 cttcaatggg ttgaaacaag atgaacgtat taaatcggga atttactctt atggtcctga
      301 ttccgtaggg gaagatttct ctcttgctgc agagatcgtg gatgatttcg aagaaaaaaa
      361 taagctgatt ggtatgttag catgcacttc gaatacagga ttaacattct ctattgaaaa
      421 tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag atgaatttgg
      481 taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag atcaatggat
      541 gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta cggccactca
      601 atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa attgaactaa
      661 acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta ttgatgatca
      721 atagagattt taacacagac gagtttgcaa aaatgactgt gggaaaagga tccatggata
      781 tttctgttat tcaaggtgca aaggctgtgt atattgctta ccgccaagcg aacatgactg
      841 attatatttc attcgatgaa tttatcgata aatgggattt tgatatggct actgccagct
      901 atatttatca attgatgatg ttcaaacaag cacgcgatgc ttatcaaaaa gaatttgaaa
      961 aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag ttgaaacgtg
     1021 ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt tggttttaag
     1081 cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagtgctt atgaaggatg
     1141 gaaaaactat gcaataaacc aatcccagaa aaactaaaga aaggaggtaa aaaatggcta
     1201 aaaagagaac agaagcagaa gtaacattca tagctaacga tgacggattg aaatctacgt
     1261 taaaagaaat cagtgctgaa ttaactaaaa atagagcaga attaaaacta gaacaagctc
     1321 aattacaaca gactggttct gaatcagaca agttaggaag taaattatct tctttagaaa
     1381 agcagtatga attacaaagt caaaaagttg aagtaactag ccaacgttta gccaatgcca
     1441 aaaaatatta tggagaaaat tccaccgaag ttcagaaact tgagagagaa ctgattaacc
     1501 aacaaacagc gcaacaacgt ttgtcaaacg aaattgataa aacgagtaat gcactagctc
     1561 aagcaaaagg cgaaatacag acgtacgagt ctacaatgca acagttggat agtgaacaaa
     1621 aaaatgttca agctagtgct tctctgattg aatccgaata caaaaaatgg caagcaactg
     1681 ctggtcaatc agcttctgaa tccgagaaat tagcgaaagc ccaagaatat gtttctcaac
     1741 aatctgaaaa tgcagagaaa acgatagata tcctgagacg acagttagaa gctacacagt
     1801 ctgagtttgg cgctacatcc acagaagcaa tgcagatgga ggcgaagctt aatgatgctg
     1861 aacgtgaatt tgaagagtta ggacaagctg ctaaaaatgt agatacaact aacttggacg
     1921 atatcggaag caaaatagat atgaataatc taatggaagc ttctgacgtt ttaagcgaca
     1981 ttggcgataa gcttacagaa ttagggaaac aagcagtgga ctctgctaat agtgtaggta
     2041 gttcccagag taaaatacaa gctaattttg gtttgactaa acaagaggct gaagaattaa
     2101 cgaatgtagc cagagacatt tattataaag gttttggaga atcgttagat cagtccacag
     2161 atgcattgat tttggtaaag cgtaatttag gcgatttaaa taatcaagat ttacaaaata
     2221 tcacggaaca agctatggtc ctagaaaaca ccatgggcgc tgatatggat gaaacgttac
     2281 gtggtgtaaa tggcttaatg gtcaatttcg gcttgagcgc tcaagatgca atggatttaa
     2341 tggtttcggg tactcaaaac ggtttagata aaacgcacga attaggcgac aatatggcag
     2401 aatatagcca attatggagt caaatgggat attcagctga tgaaacgttc ggaatgcttc
     2461 aaaatggttt agatgcgggt gcttataacc ttgataaagt caatgactta gttaaggaaa
     2521 tgggaatatc gttaacagat ggtcgatttg agcaaaacat ggatatgttt agtgaaagta
     2581 ctagaaaagc ttttgaagag tggaaaaatg gcggaggaac acaaaaagac gttattaatt
     2641 ccatgattca agattttagc aatatggatg gtcaatacga ccaattaaat aaagcttcta
     2701 caatttggtc tgcacttggc gaagataacg cgatgaaagt tgtccaatct ttgactgatg
     2761 ttaaccatac atttgatgat gttagtggat ctgcacaaaa aatgaatgaa gattctacta
     2821 ctccgttaca agaattaaac ggaaaaatag ctgaattaaa ggattcatta gctcctatag
     2881 gcaacacaat catagatgca ctcgaaccag taattgattt tctaggaaag atggctgatg
     2941 cgtttaataa tcttccacaa ccagtacagg attatgccgt agcgattggc ggattgactg
     3001 ctgcatttac tttattaatg ccaataatag ttggcttcat ggctctaggt ggtcctacta
     3061 cattaataat aggagcagtt attactgtta ttgctggagt tatagcaatt ataaaaaact
     3121 ggggcgcaat tactgactgg tttaagggaa tatggagtaa attcactgat tggttgggtg
     3181 gtacttggga aagtataaaa gaaggtgcct catcagtttg ggatggagtt aaagaaacct
     3241 ggtctggatt tgtagattgg gttcaagata tttggcaagg agtttctgat tggtttggag
     3301 agctatggag cggattagtt gaaggagctt ccaacatctg gcaaggagtc caagagactt
     3361 ggcaagcatt cgttgattgg gtttcaaata tttggaacgg agtcaaagaa gtatggtcga
     3421 ttatttgggc agacattgta ggaattgttc aaataccatg gaccttaata acgtcattga
     3481 ttcaagccgg tattaatatt atcgtgggta tttttgatgt agctggacag ttattaggcg
     3541 cagcttggca agctgtttgg acacctattt ctgatttcct taaaaacact tgggatacta
     3601 tgacacaatg ggtaagcatc gcttggaatg gaattgtaac tacattccat actatatttg
     3661 atccagtagt ggcatggtgg aatggtatat ggacagctat tagtactacg gcttcaaata
     3721 tttggaattc aattagtgca acagcttcta gtatttggaa cagtatcaag aatacaatca
     3781 ctagcttggt acaagcagct gctacagtaa ttcaaaatat ttggtcaact gtatctagtt
     3841 ggttaggtgg aatttggaat tcaatcagct ctacagcatc aaatatctgg aatagcgtga
     3901 ctagtagtat aagcaatgct ataaacgcag ctaaaagtgc cattcaaagt gtttggaata
     3961 gtatatcttc gtggatcagc ggaatttgga acggtatcaa aaacactgct ttgaatcttt
     4021 ggaatggaat tacaagcact attagctcta aagtaaacga tggaaaaaat gcaatttcaa
     4081 gcggttggtc caatctaaca ggtattgttt ccgacatatt caataatgtt aaaagtacaa
     4141 ttgctaacat ttgggaaggt atcaaaaaga ctgttagcgc tccaattgat tggatcagag
     4201 ataaaatcag tagtatcttt gataatttga atatttctat accacatatt ccgttaccac
     4261 attttaaatt gagcggagaa ttcaatccat tgaaggggaa aatcccaacg ttgggtgttg
     4321 attggtatgc gaaaggtagt gtgtttaatt ctccgaatat tatcggtgtc ggcgaagcag
     4381 gacctgaagc agttttacct ttgaaaagat ctgtgctgca agaaattggt gatcgtatct
     4441 tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat
     4501 tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa
     4561 tcattgatag cattacaaaa gttcaaaatg ataatgcttc ggcatggcgt taaacaggag
     4621 agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca
     4681 ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac
     4741 cataccttct ccaaaaagac agagagttga aacttctgtt ttaggaaggt taggtggctt
     4801 agtacaagat tactcgtttg aagacatgtc gtttacattg cactataact atttagagga
     4861 tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaatta
     4921 tgcaaagaaa ttagaattct ctgatgatcc caacgtctat tatgttatcc agactatcga
     4981 tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc
     5041 gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt
     5101 taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc
     5161 tacttcatgc cagttcatct taaatgatta tgtgtttagt tttgaaggct tagtagtagg
     5221 aactgacgta gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga
     5281 tattttagat cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat
     5341 taattatttt aattgtactg gtttgagcaa aatacaaatt tatcgtaatg ggttaaggta
     5401 ggtgaaatag atgatcgata atttaataac tatttacgat aaaaacgacg cgaataattt
     5461 agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac
     5521 tgttagcaat aaattaaacg gagccgagat atttcagggt acttatccaa taagcggaac
     5581 taatgcagat ttgattgtag aaggacgtat tattcagtgt tatgtagatg aaaatcgagc
     5641 aaaacagcgt ctacggattt attatgcaaa gacttctgta ataggcaata cgatagaagt
     5701 aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat atgacagtgg
     5761 aacagaaaag atcactgcta gtcaggcatg gcaaaacgca aaaactttag cgaaaccagt
     5821 tattccttca cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga
     5881 aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca
     5941 tggggaattt ctaaaagata ataacacatt acgtcatgaa aaaaggctag gcacggatca
     6001 taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca
     6061 aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat
     6121 cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt
     6181 ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa
     6241 agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt
     6301 agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat
     6361 gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt
     6421 ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa
     6481 aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga
     6541 tcaattgaaa aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac
     6601 tgatatgatt actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag
     6661 tcgtattttg attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa
     6721 caatgctggt attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac
     6781 gttagatggc ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat
     6841 tagaggtact acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa
     6901 aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta
     6961 tgtaagtgct gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat
     7021 tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt
     7081 tctatctttt ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga
     7141 cggtagttct ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg
     7201 taggactatg aaatttacta gtgatggtat gctaacgtta ccaggttgtt tttttggttc
     7261 atgggaagat gggaaacttg ctaggtttga acaatcaacg gtacaagtat ataaagattt
     7321 tactgttaga ggtactaaaa actcaactgt accaacagaa cattatggac aacgactatt
     7381 gaacgcttat gaaactccag aatattattt cgctgattat ggggaagccg ttacaggtga
     7441 cgatggtaaa gttcgtgttg atattgaccc catgtttgct gaaacagtaa atctaagtcg
     7501 gtatatgaca catgtgacac ctacagaact agttttgtgt gctgttactc atgaagatat
     7561 tgaccatttc atcattgaaa ctagtaagcc aaacgtatta gttagatgga atttagtggc
     7621 acaccgtcta gggtatgaag atattagatt aaaagaggat acagcatatg atagcacagt
     7681 gcttgaccaa aaacgttttt aaaacgaaga caaggaggta tataaatggc tagcagttta
     7741 tataatttgg ctttagattt cagcaaagaa ttaaactaca ccaaagctat tatggctcgt
     7801 caaggtgata aagggattac ggtgacggtt aaaccgtttc taaatggctt gcagatggat
     7861 acgagtggcg gaacatttac tttaaaagga acaacaccat ctaaccgtta cgtagataat
     7921 gttgcaacta gtgtaactag tgaagaagtc acgttttctc ttgatggcac atttatgagt
     7981 gaagcaggat attataaaca ctgctacgta gaatatagaa aagacaatca aattttaaca
     8041 acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat
     8101 gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt
     8161 atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt
     8221 caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt
     8281 aacttacaag tgatgtacag taacagcatc gacttcgggg gatatgatta tagcggtaag
     8341 ccgaatttaa tgtccaaact aaaatcgagc gattttaacg ttggttacca cgggtcacta
     8401 actttggata acgaaaagtt acattttact tctgatggta caggaagcat tattatgttt
     8461 acgcgtatta atacacctca gcttactagt gggaaaacct atactctgag tgcgaaagtt
     8521 cgatttgatg aaggaactac aggagctatt gataaattac gtttggtgta tcgtacatca
     8581 ccaggagaaa agatattatt ggaagcaaat agtacaaata ttacaacaga tgatgtaggg
     8641 aaagaaataa caatcaaagg tacagctaac gttaattatc aaatcacaaa tttagatcga
     8701 ttttatatga gtattagctt tgttgacagg gataaaataa atggcggatt taagttgtac
     8761 gacatcaaaa tcgaagaagg ctcaacagcc acaccgtacc agcctaatct acttgatgca
     8821 ccgtattatt tgagtaaggt ggctttgggt gagaatctaa ttaaaccaga atcacaacaa
     8881 ccagttacta atagtaacta tcttattaac acctataaca ttaaaccaat ggtaaaaggt
     8941 aagaagtata ccatcacact tgaaggaact aagccagcaa cacaggtttt tagaccattt
     9001 ttcacacgag caacaggaga tgcatgggag gttggtgact tacaaccagt agaaggctta
     9061 actaatgtgt ggtctaaaac atttacagca gcagatgact cacaccctac taccccacaa
     9121 gtgcagattt atcaagtacc aagcacaagt gtaggacaat gtacaattaa gtggttaaaa
     9181 ctagaggaag gcaatactcg aaccccgaat attagtgagt ataaatatcg tggtactggt
     9241 atgcgtgatt caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa
     9301 gataatttgg ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc
     9361 gatgggaaag tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc
     9421 acagctaagg atgctcaaac aaaagcgaat gctaatgcga ctgctataga tgaattagac
     9481 aataagatcg atgaacgcat taatgataca gctactacca cattaacagt tacaaacggg
     9541 aataccggat cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct
     9601 ttaaacggaa aacgcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat
     9661 cggccaccaa ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt
     9721 gctcagttat ctattggtgc agatggagcc attaaatggc gaagaaactc aagttatgga
     9781 tcggattata cctttgcaat tacttacacg atttagaaaa gcgtgaatcg atatgaaggc
     9841 agcatataga ccaattgaac cttacggatt cgagcaaatc attgtgaatg atgaagaaca
     9901 tttaccggaa gaatgcacag aagtcgaacc accgattcca aattggaaac cgaaattcaa
     9961 ttactgggag ggaaataaat gaaaaacatt tggaaatatg gacgtactgg cggagagtac
    10021 gcaggaaaag tattggacga catgcttgta tccgttcctt acacagatca gccaccgctt
    10081 gaaggaattc gtgctgatgg cgaaccgtta acaatcgctg atcaaatgtt tgatcctaaa
    10141 ttgaatcaat ggattatttt agcgaacgca ctagatcaca acgatttaaa caatctcaaa
    10201 gcaatgtatg agtcgttaga aaatgagaac ggcgatttaa aacagatcaa tgccaaactc
    10261 atgctaagcg atgtagcaat taaacaggaa aatactgcat tgaaagaaaa agcggatagt
    10321 ttagcacaaa tcaattcaaa aatgatgctt gcttcgttac aaaatagcaa agacatttca
    10381 gaaattaaag agcaactaaa tccagcttca aagggaggtg agtagtatgt ttagttttag
    10441 cgatgtgaaa atgatgtatg attggggctg tttcactaac gaacaagtaa tggttttcgt
    10501 tccgttgtgc attactgaag aaaaagcaga taaaatcatt agcaaagaag agagcgcatc
    10561 ttaattgatg tgctttttat tttgattcaa ggagttgtca catgattaat ttaggggaat
    10621 ggggagcgat agcaggatca ataaccgcta tcgtttcttt gattttatta gtaataaaac
    10681 caattactgc atctttctcg aagattactg agactctttc aaaagtaagt cacaatttag
    10741 atttgctgac taaagattta gaatcgagca aatcagatcg attgatgatt catgaagaac
    10801 taaagaaaca cgatgaaaga ttagatacac atgcagaaaa attggtagaa cacacgcaac
    10861 aaattaaaac tttatttagg gagagaagaa aatgaataat aaaacgttcg aagtactaaa
    10921 atggttcgca ctggtaatta ttcccgcact agctactttc gtggggttag ttggtaaagc
    10981 gctcaattgg cagtacacag atatctgtgt tgtcatcatt actggttttg gcgcgttttt
    11041 agggagtgtg ttgggtgtat caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga
    11101 aggaggaaac aaatgaaaaa gaaaattact attactgcga tgagcctgtt aacggctctt
    11161 tttttattac ccattaatac gtttgcttat actattaatg acgagtataa tttagcgccg
    11221 aatcaaggag actccagatt agcaattcct aacaaaatta ttttgcacga aactggaata
    11281 gatgcaccag caagaaacgt agccgccaac atgaaaaata attataacgg aagcaattct
    11341 tatactacag atgttattgg tgacggtggg attgtttacc gtgtgggtga gcaaggatat
    11401 gtttcgtggg gagctggtaa cgctaatcct tatgcgcctg tacagattga attacagcgc
    11461 acatatgata aagcattgtt tgaaaaaaac tatcgagctt atattgaata tacaagagat
    11521 agtgcaaaaa aatatggaat tccattgact cttgatcaag gaacttcttt atttacaaaa
    11581 ggaatcattt ctcatttgtg ggtgacaaat tatgtttggg ggaaccacac agatccatat
    11641 ggttacttat cgcaaatggg agttagcaaa gaaaagcttg cttatgattt agctcatgga
    11701 tttaccgatg aaaatccaac aacttctgaa aacaagcctg tcattgatcc aacacgagct
    11761 ggtgcagcta atcctacgct gacagatgga acaaatcacg cccacattga tcagtttggg
    11821 gaaatcgaaa acgcaaactt gcatgtggct ggatggcaca ttgctaacta taaatacgag
    11881 tatattttca ttatggacta caatactggg aaagaattag ctcgagtaag agctgatgga
    11941 atttatagac cagatgtaaa ccaagcttat aatacttctg gaaatgttgg ttatcatgta
    12001 tctttcaata tgcgtaattt tcctagtaag aaagtctatg taatgatgcg ggcaacgaat
    12061 gatccagagg gaaacactaa aggcggtgcg caagatttcc atgacaaacg ttggtattta
    12121 aatattccta aacgataaaa atagctcctc gttgaggagc agtacataac tatattgaca
    12181 actataaaaa ttattcgata aaatagtgat gttatcgcat atcttcacta tcacccataa
    12241 atagtcacac tccaagctat gcgataacag gtttgttgcc acacattcta ctggttgatt
    12301 gtttatggct ttatgtggca acaaccagta cccttagctc agttggtcag agcagacggc
    12361 tcataaccgt ccggtcgtag gttcgagtcc tacagggtac attaacgtag ccatttgaat
    12421 cgttctgtgt tagaattttt ttgaagagta ttatacaagc taaagctttt cttcattgcc
    12481 actcaaatga gtggcttttt tatgtatcct tttatggatt aatgaaagga tgtttcacat
    12541 agttatactt ctgtata
//
LOCUS       DACCVU010000086        12156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCVU010000086 DACCVU010000000
VERSION     DACCVU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.086"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1042)
                     /locus_tag="IVW29_002065"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IVW29_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IVW29_002066"
     CDS             complement(1437..2279)
                     /locus_tag="IVW29_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7839543.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IVW29_002067"
     CDS             2604..3020
                     /locus_tag="IVW29_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7839544.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IVW29_002068"
     CDS             3017..3487
                     /locus_tag="IVW29_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839545.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IVW29_002069"
     CDS             3501..4274
                     /locus_tag="IVW29_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839546.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IVW29_002070"
     CDS             4288..5100
                     /locus_tag="IVW29_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7839547.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IVW29_002071"
     CDS             5154..6119
                     /locus_tag="IVW29_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7839548.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IVW29_002072"
     CDS             6256..8979
                     /locus_tag="IVW29_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7839549.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IVW29_002073"
     CDS             8992..9624
                     /locus_tag="IVW29_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7839550.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IVW29_002074"
     CDS             9829..10056
                     /locus_tag="IVW29_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP7839551.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IVW29_002075"
     CDS             10125..10391
                     /locus_tag="IVW29_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7839552.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IVW29_002076"
     CDS             10381..10650
                     /locus_tag="IVW29_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7839553.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="IVW29_002077"
     CDS             complement(10829..12130)
                     /locus_tag="IVW29_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7839554.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3971 a         1972 c         2384 g         3829 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgt
//
LOCUS       DACCVU010000087        12060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCVU010000087 DACCVU010000000
VERSION     DACCVU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.087"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..570)
                     /gene="frr"
                     /locus_tag="IVW29_002078"
     CDS             complement(13..570)
                     /gene="frr"
                     /locus_tag="IVW29_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7839555.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(573..1295)
                     /locus_tag="IVW29_002079"
     CDS             complement(573..1295)
                     /locus_tag="IVW29_002079"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7839556.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1431..2312)
                     /locus_tag="IVW29_002080"
     CDS             complement(1431..2312)
                     /locus_tag="IVW29_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7839557.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="IVW29_002081"
     CDS             complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="IVW29_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7839558.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3552..4031
                     /locus_tag="IVW29_002082"
     CDS             3552..4031
                     /locus_tag="IVW29_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7839559.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4248..5339
                     /gene="phnW"
                     /locus_tag="IVW29_002083"
     CDS             4248..5339
                     /gene="phnW"
                     /locus_tag="IVW29_002083"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7839560.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5332..5460
                     /locus_tag="IVW29_002084"
     CDS             5332..5460
                     /locus_tag="IVW29_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7839561.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5464..6009
                     /locus_tag="IVW29_002085"
     CDS             5464..6009
                     /locus_tag="IVW29_002085"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7839562.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6463..8154)
                     /locus_tag="IVW29_002086"
     CDS             complement(6463..8154)
                     /locus_tag="IVW29_002086"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7839563.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="IVW29_002087"
     CDS             complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="IVW29_002087"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7839564.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9636..10655)
                     /gene="argF"
                     /locus_tag="IVW29_002088"
     CDS             complement(9636..10655)
                     /gene="argF"
                     /locus_tag="IVW29_002088"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7839565.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="IVW29_002089"
     CDS             complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="IVW29_002089"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7839566.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3289 a         2510 c         2188 g         4073 t
ORIGIN      
        1 tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac
       61 ttctttgatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc
      121 ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat
      181 gttacgaatc gcgatttttg cattttctgc ttcttttttc acatctttag ctaattcttt
      241 tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg
      301 actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc
      361 aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa
      421 tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg
      481 gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc
      541 ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata
      601 ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt
      661 ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg
      721 gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga
      781 tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt
      841 tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct
      901 gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc
      961 tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc
     1021 aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct
     1081 tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc
     1141 acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct
     1201 ttgattactg gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat
     1261 tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt
     1321 gccattttac catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt
     1381 aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc
     1441 acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact
     1501 tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc
     1561 atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc
     1621 aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt
     1681 tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca
     1741 taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt
     1801 gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat
     1861 gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata
     1921 acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt
     1981 gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa
     2041 tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct
     2101 gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc
     2161 ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct
     2221 tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt
     2281 tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt
     2341 ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca
     2401 tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga
     2461 ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt
     2521 ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat
     2581 cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag
     2641 ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt
     2701 caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt
     2761 caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag
     2821 ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat
     2881 cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag
     2941 ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc
     3001 cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt
     3061 tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg
     3121 gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag
     3181 aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta
     3241 ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg
     3301 atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat
     3361 acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag
     3421 tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga
     3481 cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa
     3541 ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat
     3601 tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca
     3661 cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa
     3721 atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg
     3781 aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact
     3841 ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg
     3901 agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct
     3961 cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat
     4021 cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata
     4081 ttttccttat tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat
     4141 ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca
     4201 gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat
     4261 taacttctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt
     4321 gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc
     4381 ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg
     4441 gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa
     4501 acggcgcata tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg
     4561 tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag
     4621 agaatccgtc tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga
     4681 ataatttgga agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg
     4741 cgatgtcaag ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga
     4801 tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata
     4861 aagcggaact ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc
     4921 agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg
     4981 ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat
     5041 attcacgtta tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc
     5101 agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact
     5161 ggcgatttga ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc
     5221 ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg
     5281 aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa
     5341 actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac
     5401 gcttttagaa atgcttttct agaaaaagga acccaactga ctgacaaaga aatccgctaa
     5461 ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa
     5521 aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat
     5581 caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac
     5641 acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga
     5701 tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca
     5761 gacttcatgg caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc
     5821 cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat
     5881 acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa
     5941 ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag
     6001 atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa
     6061 acaatttaaa agagttaaca gaatacattt tgtacgctga caagaatcca gaatgacttt
     6121 tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc
     6181 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
     6241 aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
     6301 atttgcaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg
     6361 actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct
     6421 ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc
     6481 atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc
     6541 taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt
     6601 gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc
     6661 agcagacaag accgtttcag ggtatttttg aactaattta accacttccc agctggtatc
     6721 gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct
     6781 gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac
     6841 ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac
     6901 tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc
     6961 gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat
     7021 ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg
     7081 gtgcatgtcg tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa
     7141 atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt
     7201 acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt
     7261 aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc
     7321 gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa
     7381 agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc
     7441 tttcatcgat tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc
     7501 cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc
     7561 atggaattgg acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc
     7621 tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa
     7681 atggttgatg cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc
     7741 gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat
     7801 gtcgattggt actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt
     7861 cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta
     7921 aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc
     7981 agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac
     8041 atctccgtgt tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa
     8101 atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat
     8161 tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta
     8221 gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga
     8281 ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc
     8341 ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata
     8401 caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa
     8461 aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc agatggccta aaggagaaga
     8521 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8581 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8641 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga
     8701 agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8761 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8821 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     8881 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     8941 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9001 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9061 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9121 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9181 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9241 gcgttttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9301 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9361 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9421 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg
     9481 gcattgcccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9541 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9601 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9661 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9721 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9781 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9841 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
     9901 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
     9961 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10021 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10081 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10141 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10201 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10261 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10321 ctgaatccac ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct
    10381 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10441 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10501 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10561 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10621 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10681 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa
    10741 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga
    10801 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10861 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    10921 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    10981 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11041 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11101 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11161 tccaaatgca tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct
    11221 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11281 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11341 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11401 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11461 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11521 gggtccatgt aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt
    11581 acttcgatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11641 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11701 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11761 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca
    11821 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    11881 tctggtgtca aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc
    11941 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12001 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
//
LOCUS       DACCVU010000088        12041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCVU010000088 DACCVU010000000
VERSION     DACCVU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.088"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            141..461
                     /locus_tag="IVW29_002090"
     CDS             141..461
                     /locus_tag="IVW29_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839567.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="IVW29_002091"
     CDS             913..1113
                     /locus_tag="IVW29_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7839568.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="IVW29_002092"
     CDS             1260..4049
                     /locus_tag="IVW29_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7839569.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="IVW29_002093"
     CDS             4096..4623
                     /locus_tag="IVW29_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7839570.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="IVW29_002094"
     CDS             4644..5834
                     /locus_tag="IVW29_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7839571.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="IVW29_002095"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="IVW29_002095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7839572.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7967..8641
                     /locus_tag="IVW29_002096"
     CDS             7967..8641
                     /locus_tag="IVW29_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7839573.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8638..8979
                     /locus_tag="IVW29_002097"
     CDS             8638..8979
                     /locus_tag="IVW29_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839574.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9009..9368
                     /locus_tag="IVW29_002098"
     CDS             9009..9368
                     /locus_tag="IVW29_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7839575.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9562..9750
                     /locus_tag="IVW29_002099"
                     /pseudo
     CDS             9562..9750
                     /locus_tag="IVW29_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9892..11976
                     /gene="cadA"
                     /locus_tag="IVW29_002100"
     CDS             9892..11976
                     /gene="cadA"
                     /locus_tag="IVW29_002100"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7839576.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4012 a         2108 c         2595 g         3326 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5341 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5401 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     5581 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7201 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7261 gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt ctttttttta
     7321 aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
     7381 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
     7441 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
     7501 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
     7561 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
     7621 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
     7681 gcctataaat atatgtttcg atggcgtcat cagtggtctg attgacggaa gcttgggtgt
     7741 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
     7801 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga aggaactagg
     7861 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     7921 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     7981 attatttatc ggtgatagca ttacagacgc aggaagatac cgagcagaag caactgatct
     8041 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     8101 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     8161 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     8221 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     8281 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gcggaaatct gcaacatcat
     8341 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     8401 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     8461 tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat atacaagaga
     8521 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     8581 aattgtcgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgaaaaatg
     8641 atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct gattactgat
     8701 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     8761 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     8821 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     8881 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatacttgat gctggaagct
     8941 aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt aatagaagga
     9001 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     9061 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     9121 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     9181 atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt tcaggcctac
     9241 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     9301 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     9361 ttgtataaaa gagaataata aaggttagat aaagcataga gccaggtggg atacatgtat
     9421 ttcacctgac tctatgcgtt ttcctgtata agttcttcta tgtgactttt acgtctttta
     9481 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     9541 tatttttgaa agcgtagtgg agcttcgtat atctgggaag gacattttgc agggattgaa
     9601 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
     9661 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
     9721 gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
     9781 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccattcattt
     9841 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
     9901 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
     9961 aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
    10021 gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt gaaggaatta
    10081 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
    10141 aaaaagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
    10201 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
    10261 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
    10321 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
    10381 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
    10441 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
    10501 gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga tcggtcagtt tgtacttgtg
    10561 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
    10621 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
    10681 agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
    10741 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
    10801 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
    10861 ttattagctg ttttacctcc acttgttgtt tctagagcgg cgttttctga atggatctat
    10921 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc cgtgccactt
    10981 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
    11041 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaactgg cacattaacg
    11101 aaaggtgact tttcgatcca gacgatcgat ccaacaggaa ttgatccaaa gaaatttatg
    11161 caatatgttg caagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
    11221 ggctacgcag gtccgctttt accaacagct aatatccagg aatttgcagg tgaagggatc
    11281 ttagctgaag ttgatggcaa acaagtatta gtaggtaatc acaaattgat ggaaagattt
    11341 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
    11401 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
    11461 gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt tgacgggcga ctcgaaaaaa
    11521 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
    11581 gaagataaag tgcagcggtt agaggaaatc ttacaaagta acaataaaaa gaccgcattt
    11641 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
    11701 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
    11761 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
    11821 aacattgtct ttgctattgg gataaaaatt ttggtacttt ccttaggtgc ttttggtttt
    11881 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
    11941 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt
    12001 cctctttttt ttatatcctt ttcttaatga aaaatgtaaa g
//
LOCUS       DACCVU010000089        12014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCVU010000089 DACCVU010000000
VERSION     DACCVU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.089"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(141..467)
                     /locus_tag="IVW29_002101"
     CDS             complement(141..467)
                     /locus_tag="IVW29_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839577.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(504..869)
                     /locus_tag="IVW29_002102"
     CDS             complement(504..869)
                     /locus_tag="IVW29_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7839578.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(906..1121)
                     /locus_tag="IVW29_002103"
     CDS             complement(906..1121)
                     /locus_tag="IVW29_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839579.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1250..1585
                     /locus_tag="IVW29_002104"
     CDS             1250..1585
                     /locus_tag="IVW29_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839580.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1661..3658)
                     /locus_tag="IVW29_002105"
     CDS             complement(1661..3658)
                     /locus_tag="IVW29_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839581.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(3756..4031)
                     /locus_tag="IVW29_002106"
     CDS             complement(3756..4031)
                     /locus_tag="IVW29_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839582.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4263..4997
                     /locus_tag="IVW29_002107"
     CDS             4263..4997
                     /locus_tag="IVW29_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839583.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5036..6178
                     /locus_tag="IVW29_002108"
     CDS             5036..6178
                     /locus_tag="IVW29_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7839584.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6658..7143)
                     /locus_tag="IVW29_002109"
     CDS             complement(6658..7143)
                     /locus_tag="IVW29_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP7839585.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(7145..8581)
                     /locus_tag="IVW29_002110"
     CDS             complement(7145..8581)
                     /locus_tag="IVW29_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839586.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(8614..9465)
                     /locus_tag="IVW29_002111"
     CDS             complement(8614..9465)
                     /locus_tag="IVW29_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839587.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(9621..9866)
                     /locus_tag="IVW29_002112"
     CDS             complement(9621..9866)
                     /locus_tag="IVW29_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839588.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            9971..10744
                     /locus_tag="IVW29_002113"
     CDS             9971..10744
                     /locus_tag="IVW29_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839589.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(10797..11111)
                     /locus_tag="IVW29_002114"
     CDS             complement(10797..11111)
                     /locus_tag="IVW29_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839590.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         3981 a         2171 c         1646 g         4216 t
ORIGIN      
        1 cactgctgtt ataacagtga ctaatgtaaa gattttccca gcaatcatca gactattatt
       61 tacagggaat caaaaaaact ccttagtaag cttacacctt taggagttcc tgtttgaacg
      121 gtatgcataa tacatttaaa tcaatttaac attaacacct cagtatatgg tatctctaca
      181 tatacacggt ctttttttgg atagtactcg acatttatat tgatagcact tccattgtct
      241 aacgtagctt tagcccggta cgtgttatca ccattatcat gagtgatcgt cacatcatca
      301 catgtccgat ttaagttata ctgttcttgt aaaatctgag ttaccaatgg tacagatgac
      361 ttctctagtc cagatgaacc cgtcattctt gaaaaaatta tacctataat agccaataca
      421 acgataccta taatgacaaa cttcttattc atatttactt tattcaactt tttatctcct
      481 atctccaaaa tgattttctc tcatcagtaa ctcattgaca tccccaatag tacaccaaag
      541 atggctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc tttctctcga
      601 tataagtaac ccataataac agcaacagca ccaaatacca caggaataaa tgctaatgat
      661 actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg
      721 atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgt gtctgttccg
      781 gttgtctcat ctgtttcttg taatgctccg caaaactggc aatacttact ttcatttgtt
      841 atttctttac cacattttct acaatacatg tcttctcctc ctaaaatcat tcaattgctt
      901 attagctaac tgtttacttt attaaataca tcagaaaaca gctcatttat tggtacactt
      961 aatatctcag aaagcatttt catttgatag ggtttaggaa tcgttctccc tgtttgccac
     1021 gaacttacac attgctgtgt aacattcatt tttttggcta actcagtttg ggaaagtcct
     1081 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc cttctgtttt
     1141 attatataca acaatatgat gtatttcaac tcataaaata tataaatact tcaaatgtca
     1201 ttacaaatac aacaacttgt tgtactctac tgagaagggg ggtgtattaa tgaaacagaa
     1261 gcaatatgca aaggtcatta gagaaaagcg aaaagcactc gggtggacac aaaaagaatt
     1321 agctaaaaaa atcttctcta cacaacaggc agtagcaaga tgggaaaatt ctgtaacaga
     1381 acctaacctc gatagcttaa cagctctttc tagagcccta ggaacccctg taagccattt
     1441 tctagacaat gttgacatag attatgagga agagtttttg gctctctatc gctctcttag
     1501 tactgaagat gcaattcgaa caattgacta tatgaagcta ttaaaaaggc aagaaaatga
     1561 acgcaatcag ctattgaaag attgaaaaat aaataaccca ccgaacttta acggtgggtg
     1621 tattgagatg aaataagatt aagagctatg gataacctgt ctagtttaat ataattgggt
     1681 ctcttctatt ccttactctc cgtccatcat ctcttgttac acttgtaaat tgataaatag
     1741 gaactaattc aactcctgat aatatcaatt cttttattac ttcatctatc cctgcccctt
     1801 tcgctagata tactaaaaaa ctactgcttg ctttcacatt gtatagtttc ttaatatcct
     1861 tagaagctcg ttcaagagtg aatccttgtt taacaaagta gcctctgttt tctattaaag
     1921 catttttcac gctgttatcc ctgacatttc gtaggcgttc acaaacagtg ttaataagtt
     1981 ctttttctgt cattagatat gtcccttgcc acgataagcc ctgtaaaact tcatgtgctt
     2041 catctaaaat caattgtttt tccttgctaa gttctgatcg ggaattttga ggaaatactc
     2101 ttttagcctc tttttcgtta aaacatatct taacccattc ataagaaaaa tattgatatg
     2161 taaaatgaaa tttttccaga agtggaagga tatcttcatt caatttttga atcacagaag
     2221 ttgcattaaa aacgtctaat cgatagacat tgcttctctt atgtttaaag tttcctaacg
     2281 ctttctcttc ctttaaccta tttaggtatt cgagcatctt caaaggaaca tcttcatcat
     2341 ctaatttttt tatcaatcct ataaaagtca taagtgatag taacttatta catttatcta
     2401 tgtcataagt tttaccatag taagaactca tttttttagc taattgttcc cccgacaaaa
     2461 tacaagtcac actttcctta ctatcatcaa aaaaattaac atcagaaagc aagacatcat
     2521 agacacgtcg catttcctct agataattcc ttattgtcct gaaaagtttg tctttcattg
     2581 attcaagagt gtcaaaaagt acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat
     2641 acaagtctag gttctctaat agccaattaa atgtttctac ccgactgtct ttctttaaaa
     2701 ttagcatcac aactccaata aaattttcac tgatatcaca attactggaa tgacaataaa
     2761 aaatggaatg cccagtttta ctggtaaaaa tactagcaga gggattatga tcgttgtgaa
     2821 acagacatct taagttttta cttgacttaa gtaatttatt catatcaatg gttaacaatc
     2881 tttcataaat ctcattaaaa gacatgtcgg aggaatcaaa caggcagtct ttaaaccact
     2941 gtttcatttc ttctttgtcg tcatttttta tcaacttaac aaaatcggtt tcattgacta
     3001 gttcatactc tctccctgaa tcactttgac aatttttaga acatgtcatg gtaaaatcaa
     3061 tcggaagctc agtaatatct aacatgttgt cttcattaat tattatcact gcatctgact
     3121 tatatcctcc aaaaaacatt ctattcgaag catttacatt gtcatcacac ccggggatgt
     3181 tctttttcaa gtattcgtaa acagcaacca ttttaccata gtcagtaatt gcttctttta
     3241 atagaaagac aatcttgtat ttttctagat tttcagagta ccgaatactt ttttgtatcg
     3301 caaacgcaga attatgaact aaattgatag ataaaacctt ttctatgctt aagtatctac
     3361 tatcatcttt actcaaatct actaccttcc ccttctcatc tttaacagtg ttatcgacat
     3421 ccagaataat tactctggtt tccacaaaac tctccttgtc attgccttta ttctttgtct
     3481 tgcttggaat aaatgattgt gatgaattta gtatctctac aaactcatct cttgtaactt
     3541 ctctatagtt atctaaacac ctatctctta catcagtttt ttcttttttt gtaactggct
     3601 tctctaataa tttttcttta tctacataaa ttggaatatt aatcattgat gtttccattt
     3661 gtgtttcctc cttataaaat tctgttaaag actaaaacat acagataata aaaacaaact
     3721 ctttattact atatgtttta gtcttatttg tttactcaac ctttataccg ttccatgaaa
     3781 ctatcaatat cagatttttt aaatctaatg accgtatcaa tttttataac aggtaacccg
     3841 tattgagaaa catattttct aatcgttcct actgatacac ctagatattc acttgcctgc
     3901 tgatagtcca tatactctag agtgactcca gaaataatgt cttctagcat aggctttagc
     3961 actgtaatta tctctttcac ttgttgttca acttgtatag aaacaatgct atcaatgatt
     4021 tgtagagaca tatttccccc tcctttcgtg attgcttgaa caaattttct aagaattatc
     4081 ttcgtatttt ttgctcaaaa taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa
     4141 tgattaccag ttcatcgaca tacacataat acctcgataa aaagggggtg tcaatagttt
     4201 tgtaaaattt atttacaaca cccctttttt gaggtatact aattttagga ggtgtggtca
     4261 atatgataaa acgtccacca ataaattatt tagaacgaaa aaagatactc ggaacaaaaa
     4321 taaaagctat aagaaagagt aaaaaattaa cacaaccagc atttggtctt atgattaaca
     4381 acggtcaatt aattgacaaa aagactatct acgaatggga gaagggaacc tatttaccaa
     4441 taccagagcg attatcccga attgctgatt tgggaaatat gagtatagag gagttagttt
     4501 gtggcaatgt agaagaatat attttgggaa ttattcttta tagagatagc attgtactag
     4561 atggtataac atttccagat aaaaacctgt tccagcatct tcgtcaacag ttcccaccag
     4621 tacactctaa tttagataca tggctggata gatattccaa attagaacca gagatgcaag
     4681 aatttattgc taacaagact tgtaataaag ttaaaaatga aaaaattagt cttttcaata
     4741 tcttaaaaat agaagaatta ttcatcaatg ctattgtaga agaatttgac aataacattc
     4801 ttttcctcac tagcagtata gaagaactac tagaaagaat ggtagatgaa tggctaccta
     4861 ttcaattgaa agacatgtct tatcccgaag aagctgtgag agaaattact gataatataa
     4921 acaagctaga acaaacgatt tcatcaattg ggaaaaaata cactaagaaa aaaatgaagg
     4981 gaggtgatac catctagtgg taagtactcc ttgtaaacta agaggagatg atttcatggc
     5041 aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg tggatgtata aaggacacat
     5101 tgccaccgat ccacgaactg gtgaaaaaat aaatactacg agaagaggtt ttagtagcaa
     5161 acaagaagct caaaaagatt atgacgagta cagacatcaa atattatacg gtgttaaaaa
     5221 gaaagcacct gatatgacat ttgaagacct gtataatgaa tggataactc atcagcgtac
     5281 cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac gcaaataatc aaatacttcc
     5341 agcatttggt aagcttaaac tatcgaatat ctctgtccca tattgtcaaa aagtggtaga
     5401 tgaatggcac agtaaatatg aaagttacga ttatatgaga aaacagactg cacaaatttt
     5461 acgctacggg gttgcaatgc aatatattga caataatcct atggaaaaaa cacttcttcc
     5521 acgaaaaaaa gagtatgaga aaaatcgtaa attttactca aaagaggagc ttaataattt
     5581 actggatgct tttaaagatt ttggtaacat gaaacaatac gccttcttca gattattggc
     5641 atacacaggt atgagaaaga gtgaagtact cgctttacag tggaaagaca tagacacgtt
     5701 taataaagaa ctacatgtca ataaaactct tgctgtagat gaatttggta aagttataat
     5761 tcaatctccg aaaacaagag cttctagacg agttatctct ttagatactg agactctttc
     5821 tatcctcaac aactggaaat tacagcaaaa agaagagtat ttaaaacttg gatacaatac
     5881 tagctctaaa gagcaacatg tttttacaac agttaaaaac actctataca ttccaaacac
     5941 tgttaatgat tggttaagat atattttgaa aaaatataac ttgcctcgta ttacacctca
     6001 tgggtttaga catacacatg ctagtttatt attagaagca ggagaatctg tcaaggtagt
     6061 tcaacaacga ttagggcatg aaaatagtaa agtgacatta gacatctatg cacatattac
     6121 aaataatgca cctaagaaaa caggtcaaga ctttgctgat atgatggctc atcagtaaaa
     6181 gagctggtca aaatctggtc atttctagta tacaggcaaa acaaaaaagc tagaaccctt
     6241 atatatcaag ggttctagct cctataacag gtttacccaa cactgccttc caatgactct
     6301 tttgggaatc agaggaaaga atcaggcttt atagagatat ttcatctaac aaattacttt
     6361 agactgacag tatatatcag caggtactgt catcgggaac gtagccaata tcgtatccaa
     6421 aacagcaggt agagaagcta tttgaggatt caagttattc tatttcaacc atgaagttaa
     6481 gttcattaaa aataaagata gttggtattt caataatttt tgtatagagt taatcacttt
     6541 attgatgcta attcagtttg atgaattaac tgataaaaaa atatctacta tttaaagata
     6601 cagggataag taagtaaaaa aataacatgt cattagatag tagacagttc caactcttta
     6661 tttacctttt ttgtatgctg cactgtcaat ccaagttcct aaattgttgt atccatcatc
     6721 acttatataa tcatattggc catcaataat ttcgtcaaac cacaaatatt ctccatctgg
     6781 tttttgccca attattttaa ttactgattg actagtagtt gtcatctggt taaaatcttt
     6841 tattttacct atattaacgg ccaaaatagc atttcctcgc ttataactct cctggatttc
     6901 atatttaaca tattctcgat caagagtttc tttacctatc agcaccacag tcactgatgt
     6961 tccaattaat tgattatcta tccagttttt tatagcaata tctccattac gtttaacttt
     7021 ctcaaattca gccttatcta caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt
     7081 tctaactacc attgctcttg ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac
     7141 catattaatc ctcctgaata ctatttatta aactgacaat ttgttcaact tttttatcta
     7201 cttctccttc agtatctaat ttttctagcg cttgaaattt actttttaag gaatcatcaa
     7261 aataatcact gaaattctct tgaatcttat caaacaaaac cttacttgca cctttggtaa
     7321 atccaatcgg aactactata ttttcatttt gaacagataa ttcaaattct tcaatcattc
     7381 catctgctgt aactatttca cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc
     7441 cagactctgc aataatgttt tctcgatatc ttctccactc atgaggcata tcaaggggaa
     7501 gtggtcttaa agaaagtacg tcttgtatgt tattttttct taaatttctt ctctgcttta
     7561 gaactcctga aacaatacta ttaccaattc caagtccgta acctgatact attctataac
     7621 cggattcaga aagcctatat gctaatttct cgacaaattt taatcctttc gattcagatg
     7681 taaatggagt ataatcaaca gcacttcccg aaataaaaat agtttttctc aaatactttt
     7741 ttctaatact tttcaaaata tcagtaatct cagaataatc atctacttca acaacgttta
     7801 tcgaaaatct ggacaaatca ttgatttgta gtcttctttt tgtcagttca taattatatc
     7861 tctcttgatc aaaaaaatcc tcagaatcga caaaattttc ctttaatatt tttctcatta
     7921 tacaataatg aggtctttgg ttttctctca aaacccatct tatatctgat aaaattcttt
     7981 ctaaattagg gtctgtaaaa ctaaatccta aaaagagaaa agtttttgtt aacaaatctc
     8041 cttttaaagt ttctttaaat aacgcgtacg aatcatcatc atatttttca taatcatctc
     8101 ttgttattac tgtcgaactg acatcatcta tatcaccatg cattttataa acaattgtat
     8161 ccacatttgg attagttgtt gctaactgag tataattttt cttaacctga gggtttttat
     8221 aagcttctcg gattgatttt tctattagat gatcataatt agttgtccaa taagtttcaa
     8281 taggtaaact tgctagtatt cgatgatttt cagatatttc taattcttca ccaaaattat
     8341 ccataagtat ctgattcaaa attcctcttg atccaccatt ttcattttta taatattgag
     8401 ccagagtaac taagtctgtt tcttttttag aatccaaccc gatttctgtt gcaggatcat
     8461 taagtagttc cggccaactt acatatcctt gtgacatcga taatcctgca cctgcaaata
     8521 ttgctgcatt tcctttttca atagcttcag agaacctctt tattaaaact ttcttatcca
     8581 ttctacccgg ttcctttcga ttcagtaaat tgattagaga ttagtgaaaa tggtgttttt
     8641 atataaaata tctaatgtat tacttccttt agtgcattcc acagcatcta aagcccattt
     8701 tttcaaatca gcttttcgta aataagttaa ttcacttaaa tttaaagaat attttatttg
     8761 cagattttct tgcgaagaat agtgctttat aatgcttcta acatcctcgg tgctcatttg
     8821 aggattactt tccaatcgtg gattcttttt ttccactatt gaagctgtcg tcagttcgct
     8881 atatagccat ggcgaatacg taaatttctg ttcattagtg atttcattgc tcaaactaat
     8941 tgaattaggt gtattaagaa aaaacagaca ttctgtacta tcaatcatat tatttaaagc
     9001 tgtagaaagc atcatatgaa catgactagt tgttacattt cttttatcat aatcatatgt
     9061 ttttgtttgt tccttatagc aatatttttg gtctattttt tttaataaat cagaactgta
     9121 tccccaaacc gaagaatcaa taaatgcagt aagaccaaaa ttttcaaata gccatgatgc
     9181 aaaacgtata gctaattttt catcagcatg agagtgagat ataaaaatat ctgcttttat
     9241 agttggaaac caatctttaa taatttcgtc tgcatctaga attttgctgt ccttagaata
     9301 ataatcaact aaatcatttt cactttcaaa tagattttct attctcttgt taatagattt
     9361 ttggttgtga tctatttgat ttactacatg tgtatatgat tgcgagcttg aggagaatgc
     9421 atttaagtca gcatttgtaa aatccaattc atatgcctta tacaaggata tccctccatt
     9481 cttgtatttt gatccacaat ttattaggca acataacagt ataccctatc gttacatatc
     9541 ccattcatta ttataccagt atttcttcgt gacacataag ggtgtaacat aataatttca
     9601 aacgaaatga ctacaagtag tcattgaagc aaatcttcat aagacacctg tagaatatct
     9661 ttgatggctt tcaattgatc caacttaatg tgttggcggc caccttcaat cttaaccaac
     9721 gtttccctag ttatagcaat tttacgaagc tgtaggtctc taatcagctc ggtttgacca
     9781 attccttttt ctaatcggac tcgtctaata ttgctaccga ttacattttc tttatcaata
     9841 attttttcct ctttcaagaa tagcacacct tttggactaa aattagtcct ttttcttatt
     9901 aatcttaaag attcggtatg ctacaatggg actagtatta gtccatattc aattttaagg
     9961 aggaattttt ttgaagaaat ttgcaatcat tctaacttgc gctttactca tcggaactat
    10021 aagcgggtgt accacttctg acacaagttc tacaagtacg gaaactagta caacaacctc
    10081 agaaaagaat tacccaatac atttttttcc ggttccagat ggatttgaag cttattatca
    10141 tgataaaaaa ctagatgttc ctttatcaga aactgaatct actgaaaaga ttttgccaat
    10201 tgaaattgaa agaaatcaag acgatataaa actaaaagct gatcttccct tactgaacag
    10261 cacacaaatt gatagacatg aaaatattaa agttaatatt gaaaaaggag cgttagcaca
    10321 actttatact gaaaaagttg aacaattttt tgaggctggt gccaataaag acttttcaaa
    10381 aataagcgat tattcggata acttttttaa tgagacccaa gatgaaatca cgaactcaac
    10441 ttttatggct gaagatatgt acaatatgaa gttgaaaaaa ataaagtcgt atgattatcc
    10501 attaaatcta acattaaata aagggatact ctcttttgta ttagcaggtt acgttgacta
    10561 tgaatataca gcagattatt tggagacgcc ccttcaacac gataataacg atattaattt
    10621 tagatttatc tacaattcaa aaagtaaaac atggttagtt gatcaattga gcactataaa
    10681 tacgggattt catgaattac ctaagcctga caatgaaact gctttggtca ttaaaacatt
    10741 ctaaaaaaga aacgttgaag gaataattat tctttcaacg tttcttttat tgtttttcag
    10801 tttacggtct tacctgagcg aataatctga tttctaaatg attggaaaac ttcttgtggt
    10861 tgataataat ctgtatctat ttcaaatgtt tctgagaata attcattgaa tttttctact
    10921 gaaatgatga ttttaccatt ctcctctcga ctgttttcta ttatttctgc ctctaattca
    10981 aataaatcta gtgacgaact aaaatcataa atcctgtcat attgaacaaa aaaatcaaga
    11041 aaaagttgct caaactcttc cttatttatc actagtgttt caagcggaac aactttattt
    11101 tcatcagcca ttcttccaat ttcctcactt tcattttcaa ttgtccattg ttgcggacgt
    11161 atcagatttt ctctatcatt tttcagtgta tagatcactt gtaatacaca acaatgcttt
    11221 gtaaacttat ttttttagtt agtcgaatct ttttaaatgt gcgtttcata ccatgcgctt
    11281 gatatatttt ctcctatttt ttaaatcgtt tatcacaact tttatcgttt tttcttctga
    11341 aaaaaatctt gtttacatcg ttacatagtt tccaaaaaga tacattacta gtagaaacaa
    11401 caagattaat tttacttccc actgaaatgg gaagtatatt ttaaatattt tttctccttc
    11461 aacttacact ataaagtatc ttaatcacac gatatgctag ccactaaatt catcatattt
    11521 gattcctttt ttaagataat gtagcatctt tttatccaat aacgcaattg tttttaagga
    11581 taaatttctt aagatggtac atttcaaaat ggcatttgac atatgaaaca aaaaagaaag
    11641 aaaagagcat aagggaaaac tgatagcacc ttcctacagg aaggtggcta agttggctgt
    11701 gccaactggt tttctttcaa aatcatttca taatttttga tatcataaaa aaatccattt
    11761 tggacctatt ttcggtcata atttagtacc tacttttggt catagtttcg tccgtagttt
    11821 tggtcatagt gactaaggct tttctgttca caaaattggt ttaaatttgc tttctttaac
    11881 acttcattta tatcatagca attataatgt ttacacaaca tcattcctaa ttttaacggt
    11941 tgttcctctt tatatttttt aagttctaca tttttttgcg tttaattact tttctctttt
    12001 ctttatattc cgct
//
LOCUS       DACCVU010000090        11645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCVU010000090 DACCVU010000000
VERSION     DACCVU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.090"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            318..677
                     /locus_tag="IVW29_002115"
                     /pseudo
     CDS             318..677
                     /locus_tag="IVW29_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(972..2105)
                     /locus_tag="IVW29_002116"
     CDS             complement(972..2105)
                     /locus_tag="IVW29_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7839591.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2096..2764)
                     /locus_tag="IVW29_002117"
     CDS             complement(2096..2764)
                     /locus_tag="IVW29_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839592.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3220..4206)
                     /gene="lacD"
                     /locus_tag="IVW29_002118"
     CDS             complement(3220..4206)
                     /gene="lacD"
                     /locus_tag="IVW29_002118"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7839593.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4219..5145)
                     /gene="lacC"
                     /locus_tag="IVW29_002119"
     CDS             complement(4219..5145)
                     /gene="lacC"
                     /locus_tag="IVW29_002119"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7839594.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5158..5673)
                     /gene="lacB"
                     /locus_tag="IVW29_002120"
     CDS             complement(5158..5673)
                     /gene="lacB"
                     /locus_tag="IVW29_002120"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7839595.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5692..6120)
                     /gene="lacA"
                     /locus_tag="IVW29_002121"
     CDS             complement(5692..6120)
                     /gene="lacA"
                     /locus_tag="IVW29_002121"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7839596.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6140..6913)
                     /locus_tag="IVW29_002122"
     CDS             complement(6140..6913)
                     /locus_tag="IVW29_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7839597.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7078..7557
                     /locus_tag="IVW29_002123"
     CDS             7078..7557
                     /locus_tag="IVW29_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839598.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7614..7916
                     /locus_tag="IVW29_002124"
     CDS             7614..7916
                     /locus_tag="IVW29_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7839599.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7938..9395
                     /locus_tag="IVW29_002125"
     CDS             7938..9395
                     /locus_tag="IVW29_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP7839600.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9988..10740)
                     /locus_tag="IVW29_002126"
     CDS             complement(9988..10740)
                     /locus_tag="IVW29_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7839601.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10955..11191)
                     /locus_tag="IVW29_002127"
                     /pseudo
     CDS             complement(10955..11191)
                     /locus_tag="IVW29_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11201..>11645)
                     /locus_tag="IVW29_002128"
     CDS             complement(11201..>11645)
                     /locus_tag="IVW29_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7839602.1"
                     /translation="HKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHL
                     FKSMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDF
                     RDMDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         3344 a         2510 c         1994 g         3797 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg gtcgtatcaa atgaaagctt
      421 atagaaacaa attgaaggag tacaaaatct ttcaaagcat gtctcggaaa gggaactgct
      481 tagataattc acttatggag aatttttttg gcttattaaa acaagagatt tttcacggga
      541 aagtttataa ctgtttcgag gaattaaaat cagccatcga tagctatatc tattactaca
      601 ataatgaacg gataaaacag aaattaaact ggcaaagtcc cgtccaattt cgtaaaacaa
      661 ccgttacggt tgtgtaaaaa tatcatttta gagcatgaaa aaaagagtgg atttctccac
      721 tccaaaaaag tctaactttt gggggtcact acatttttga tcctttttat ttgattaatt
      781 atatccagct ggataataat tttgggggca acatttctat cttactatta tcttcctaaa
      841 gtagtagact ttcttattgg gaaaaattac aacaaaaagg agtggcttag ttgacaactc
      901 ctttttgttt acaaatcaat tggatatcta taaattgtat tttctaacga attttataga
      961 catctgattt gtcaaattaa gctagacaat atgtctttat ctatgcagct caaaaatact
     1021 tccaatggtg ttcgataatt taatgatttt cttggaatat ggtttctttt cgaggcaacg
     1081 gattgaataa aggtttcatc cgtgtcccta aagtccattg ttttaggtaa gccatcctgc
     1141 ttgagcccat gcactcggta ctgatgagaa aaaattgatg cctgtttctc ctgaggaatg
     1201 ccaattccct gatcttttac ttcaataatc acgctaccct tttggtttga aatggtgaca
     1261 attaccagtt ttccttcacc atatttgatt gcattgtcaa ctaaattcga aaccgcccga
     1321 taaatccaac gatctcgtcc atgaatcagg gttggttgat cttcagtaaa cgcaaacaaa
     1381 agattcggat aattcatttt gtaggcatca caaacttccg cacagatcag tgaaacgtct
     1441 acgacttccg tgctaagatc ctctgagtta ttggataatg tcaaaatatc gtcaatactg
     1501 tcggtcatcc gatctaaaag cattaaattt ccttgatcgg catgttgccg ttctatttcc
     1561 agattcgtac gcaaaatagc gatggcattt ttctgctcgt gggtcagata actatttaat
     1621 cgtcgataat cctccaaatg accatcgaac ttatccttta gcttattaaa agccgcattt
     1681 aatgcctcgt ccttcacagt atttttagga acttgatcaa taaacgttaa atcacgcgta
     1741 atcgctaaga tttgattact gtaaattttg cgtaaaacca accaaagaat aaacgttaat
     1801 aacatgacgg cagaacaaat aaccagcacc atcgctggta aatatttaaa tagttcgtcc
     1861 tggtaagctt tatctatcat cgaaatagtc ataaattgat tgttggaaga tttcgaagct
     1921 tccatctgcg tctctgcttg aaccgtctta ccctcttcca taaaataaac atcttcacct
     1981 aaggtagatg cgatactgat tccctccaaa ttatttctca gaaagaagaa actcactaaa
     2041 atcacgcctg tcaccaacag tgaaaaagcc agcatcgtta acgtcatttt aaaatttaat
     2101 agcatagctg gtaccccttt gctcgaatgg tttttaagac ctctttttca gctgcttcag
     2161 ccagtttctt ttttagacga gccagatgca ctcgcagaac cgacgaaaaa ggatcaaaat
     2221 cttcatcgta gacatgttca gaaatttcct cagacgaaac aaccgctgga tgacgctgag
     2281 cgatgcacag taagatatca aattccttgg gtttcaggac cacctctgtc ttttgatagc
     2341 tgacccttcg acctaatgga tcaatcttca aagcaccaat tgaaatctgc ggcagcgcac
     2401 gtccatgata acgacgaata accgcgcgta ttcgtgcttt taattctagt aactcaaagg
     2461 gtttgactaa ataatcatct gctccaaaat ccaagccttt tgctcgttct tcaatctcat
     2521 cacgtgcagt aataatgata actggacttt cgacttcttc agcacggaga ctttgtaaaa
     2581 cagataatcc atctttttta ggcagattta aatctagtag tatcccatcg tatgaattga
     2641 tgaaggcctt ctcttctcct gactcgccat cataagctac atctacagaa atattctcct
     2701 gctctaggcc cttttttacg gataaaacca gctcacgatt gtcctcaacc accagtacac
     2761 gcatgtatat tcactcctca tacggattta gtaattacac ttatgctttt tagcatagta
     2821 cacgctcttc ttcaaagtcc acgttctttt tgtgaagatt tgaagaaatt catacattct
     2881 gttaaaaaac acataattaa gaatgatttt actgtttgaa aatacctaga atagaaaaag
     2941 actggaacct ttcctccagt ctcactactt tctatttgct tggttttctt gtatatactt
     3001 ttcaaagttc gttggtttat gaccaataag cctcgttaga atactgaaaa ttgccatcaa
     3061 gtcattaagt accacatgta agaaaatctc tctaaagaaa aaccccttcg ttttaactca
     3121 cttactattt tagaattcgt tcccctaata atgcagcatg attttacgct ttatgtataa
     3181 aaaaagactg caagcaattt tgcttcacag tctgatttct tatgatagtg agcttgttaa
     3241 cacttccacc ggtgtagcag tttctttcaa tacggcattt aattcttcaa tatttttacg
     3301 cccttgcgta ttcatccact ccatcgctgc ttcttttcca tcaacgatat agctttctac
     3361 accatctgcc caagtcgcac ggccgcaaag tacaccattg aaggttgagc cggcattttt
     3421 cgcgaagcgc agtgtttctt ggaacaactc tgcttttaca ccggcgctta ggaaaataaa
     3481 tggcaggtta gtggcttcag cctgttcctt gaagaagtta gccgcttgtg cctttgtgta
     3541 cacgacttcg tctgtcccat agccttccac ataattcata ttgacgggta cttccacctt
     3601 taacacatct acgccataac gtggatcaga aaattccttc atcatatcgt tgactttatg
     3661 aggtttctta atggcgtatt ctttcgaagt cgcatcagag atgttcgcgt cataagaaac
     3721 aagctccaag aagaagggta agttttcagc ctgacattct gagccgattc gttcaataaa
     3781 ggcatgtttt tgattattga tttcttctgc ttcatcgaca tcatagtaaa gtaggaactt
     3841 acaagcatcg gcgcctgctt cttttaaacg tttaactgac cagatcgata ataaatctgg
     3901 taaacggccg ggagttgacg catcgtaacc agttttttca taagctaaca gcaagccggc
     3961 attttttgct ctaacgtcag ctgctggcaa gccatattcg ggatctaata aaatcgatga
     4021 ggcatatttc gttaattgtg aagaaacaat ttctttaaat tcaacgattt ctgattcttc
     4081 tgcttcgctg cccttgaatt ttgtgatcat cttcttcaaa gccccgcgtt gatcaattgc
     4141 taaggcccca atcactccat cttcattgca tagttgcttg atatattgac gtttttcttc
     4201 gttcatttcc gtatcctcct acactttctc cactaagatt tgatcatata gtttttgata
     4261 attggctaat tggacatagc cagtcgtcgc ttcctgagca ttcagcattc ctaaaacatt
     4321 accctgtttt aacacggctt catccgaaag accttgttga atcgcagcgg tcaatccggc
     4381 aacggtcgca tcaccagagc caactggatt gacgacatca atctttggaa ttgtcacgca
     4441 ataaaaagtc tcgtgatgtt tggcaaaggc tccgtcgccc cccattgaaa ccactatcca
     4501 ttcaatttca tcaaacatcg aatctgacaa ggcggctttt aaatctgata attctgttga
     4561 gatttctcgt cctagcagac cagccagctc ttcggtattg ggttttatta gcttaggctt
     4621 tacactgcct cgcaacactt cccttaatgc tgtacctgaa caatctaaaa cgacaggctt
     4681 tggctgacaa acttgcagca tttgtcgata aaaatcagca ggcaatcctt ctggcaagct
     4741 tcctgaaaaa gaaatgattt cggctttaac agctaatttt tcgaaatgtt gcaagaacgc
     4801 ccttgcttct tgttcagtaa taacaggtcc gttttctagg atttccgtct gttttccgtc
     4861 atgtaaaaca gcgatgcaat tacgtgtctc tccgctgata ggtaaaaagt catgagcaat
     4921 ctcattttga gtcaggtcct ttttaatctc ttcacctaaa aaaccgccga ttaaaccact
     4981 agcaatcacc tccgtatcta attgcttcaa gatacgtgtc acatttaggc ccttaccgcc
     5041 agctgttttg cgaacatttc ttactcgatt gactgtgtcg agcttgaagg tttctaatgg
     5101 ataagaaata tcaatcgatg gattcatcgt tattgttaaa atcatttgtc cacccgctta
     5161 atcgtggtat tcgccacgat cccatttctc gataaattca tcgaagaaat gcggatcaga
     5221 ctgcttgtca ctggcttttt cgacgtgatc gattttggcg atcaattttt tgttttcttc
     5281 agtttcttga taatcggcat tgatgaaggc ctctaatatg tcacagatca aaaattctcc
     5341 cgtaatccga gcgccaaagc cgatcacatt ggcatttagt tgttccttcg catagatagc
     5401 agtcgtcata tcgcgaacca gcgcactgcg gataccaggt actttattga cagcgttgtt
     5461 aatcccaaca cctgttccac acaaacaaac tcctagatct gcttctccgc tagtcacggc
     5521 ctctccgact ttttttccgt agatcggata atgggttctg gtaaagtcat aggtccccat
     5581 atccaacact tcatgtcctt ttgattttaa gaaatctgat actgccattt ttgtatctgt
     5641 gacgatgtgg tcacagccaa ttgcgatttt catttcattc atccttccta attacgccat
     5701 tttatttaac atgtcgacac gtacttgatg acgaccacca tcataatttg cttgtagaaa
     5761 ttccttaaca atattcaacg ccaactcatc gccaacaatt tttgatccaa gtgtgatcat
     5821 ccgagaatta ttatgtttac gggtcatata cgcactgcgc tcatccgaaa cttctgctgc
     5881 taccattcct ttaacctttg ttgccgtgat aaagctgcca gcgccgtagc catcaaccgc
     5941 gattcctaaa ctaccttcgt tttcattcag atcatttgcg atcgccaaag ttgattccac
     6001 aaaatcttct gaagccgttt cactcttatc tactactgta ataccttctt tgatcaattt
     6061 tttcttgatt aattccttta attccatgcc gtcactgtct gaaccaatta ctacacgcat
     6121 ttttgtttcc tcctattttt tactgaatga ttttagtgaa ttgctgataa taactcttca
     6181 gctcggctga aagagtagaa tccgtaaata atttgtcgat cttgcgcaaa tcatagaaat
     6241 tataaaaatc gctatggtcg aatttgtttg catctgaaac caaacatttt tctgccgcat
     6301 tatctaaaac aattttttgc aaggtacctt cttcaatact gaaattcgaa acctccgaac
     6361 ccttcaatcc attcactccg acaaaagcct tcttaatacc gattttttga acagcttcat
     6421 tggcaattga accgacaaag gctccagtct tttcccgata tagcccaccg atcaaataca
     6481 gttcaaatct ttttatttca cttaatagat tgaaaaccgg cagactattg gtaacgattc
     6541 ggagagatga cggcttgatg tatcgtgtca ttaattcgat cgttgtaccc gatccaagaa
     6601 aaatcgtgtc gtcttcttga ttgatatgag ctgccgcatt tttagcgatg tattccttct
     6661 cttttgtatg cagcttcttt ttttcagtat gagaaagctc atttgcttct atagaagtcg
     6721 acatttccag ataatcgatt ttttgtgcac cgccatgaac gcgtaaaagc tttttcttct
     6781 gcgcgagttc ctctagatct cggcggatcg tcatatctga aacattcagt acttcctgaa
     6841 taaatttgac tgttatcgtc ccatagcgat cacacatcgc taagatcgaa cgctgtcgtt
     6901 ctgttttcag catattctgt acctccacga ccttataata aaacatttac gaacattttt
     6961 caacacatat tgttcgttta ttttcgttta tgcgttgaca atgattttgt aagcggttat
     7021 actcaacttg taaacatata aataaacata aacgaactaa taaaggagct gttttgtgtg
     7081 aatgaattct ttttcaaaga cactgtctat gtttctgaca agcagtcgca ggaagaagtg
     7141 ttcgaggaag tctatcaaga tctgttaaag aaagaattgg taaccgaaga ttttttggag
     7201 aaccttttag aacgggagcg taattatccg acgggattag atctctcacc tgtctctaaa
     7261 atattgccag atatcgctat ccctcatacc gaaagtgagt ttgtgcgaac cacccgtatc
     7321 attccaatca aagtaaaaaa tgaaatccct ttctacaaca tgatctcgcc aagcgatcag
     7381 atttccgctt catttctttt catgatcttg aacgaaaatg gtgaagcaca ggctggcatg
     7441 ttggctaata tcatggattt cattaattct gttgacgaag aaaaactgtt ttcgtttttc
     7501 aaattagagg acacagaagc gatttatcat tttttagaag aacattttta tatatagaag
     7561 tctctgccta accgcaggat aaaaaataaa cttattttaa ggggagaatt attatgatca
     7621 aaattttagc agcatgtgga gcaggcgtta attcaagcca tcaaatcaaa agcgctttgg
     7681 aaactgaatt gaaaaatcgt ggctatcaag tcacctgtga cgccgttatg atcaaagata
     7741 tcaatgaaga aatgctgtct cattatgaca ttttcgcaca aattgccaac accgacttgg
     7801 gttttgacat taacatccct gtcgttgatg caggtgctat tctttatcgt attcctgcta
     7861 tggctgagcc tgtttaccaa agtatggaaa gcgcaatcca atcattaaac aaataggact
     7921 aaagaaagga aaagcatatg aatacaatta ttgatattgc caataaagta tttacccccc
     7981 taattaattt aggtgcggca ccgttaatga ccatcgtatt aactgttatt gctttatttt
     8041 ttaaagtaaa accgtcacgc gctcttgaag gcgggctcaa attagggatc gccctgactg
     8101 ggatcggagc tattattgga attttaacta ctgctttttc agacgctatg acagcttttg
     8161 ttgaacgcac aggtatttca ttaaacatta cagatgtcgg ttgggcccca ctagcaacca
     8221 tcacatgggg gtcgccatac acgctttatt ttttactaat tatggtgatc gtcaatattg
     8281 tcatgttagc aatgaaaaaa accaacacat tagatgtgga tattttcgat atttggcatt
     8341 tagcaattgt tggtttgttc gcgatcttct gcggtgctaa tctgtttatt gcttctgcat
     8401 tagttatttt cattggtgta ttaaaaattt ggaactccga cttaatgaaa ccaaccttca
     8461 atgacttgtt aaatgcgcca gacagcaacc cgatgaccac gactcatatg aactacatga
     8521 tgaatccgat tattatggtt ttcgataaaa ttttcgacaa aatcttccct tggttggata
     8581 aatatgattt cgatgctgcc aaattgaata gtaaaattgg cttctgggga tcaaaattcg
     8641 cgattggaat ttatttgggg atttttgttg gattactagc aggacaaaca ccaacgcaaa
     8701 ttttctcgct agcctttacc gctgccgtct gtttggaatt gttctctttg ataggcgctt
     8761 ggttcatcgc agccgtagaa ccgttatcac aagggattac cgattttgcc aataaacgac
     8821 taaaaggacg taccatcaac ataggtttag actggccttt catagcaggc cgcgctgaaa
     8881 tctgggctgc cgctaacgta ttagcaccaa tcatgttgct agaagccatc atcttaccag
     8941 gaaataaatt attgccacta ggcggaatca tcgcaatggg tgttacccca gcactattgg
     9001 tcgtaactcg tggaaaatta atccgtatga tcgtaatcgg cgcgatcgaa ttaccattat
     9061 tcttatggtc cggcaccttg atcgccccgt tcgttactca aacagctaaa gcagtcggag
     9121 cctttccagc cggcttgaac gggtccgcca tgatctctca tacaacgatg gaaggcccga
     9181 ttgaaaaatt cctaggttat ttagtcggta atgcttctca aggacaaatc gaatttgtga
     9241 tatacgctgc cttagcatta ggtgcatacc ttttaatctt catttggtac gctcgtcaaa
     9301 tgaaaaaacg caacgctgca tacgcagctg aaaaagaaga gaatgcatct ccggcaacgg
     9361 cgcataatat gacttatgca gccgaagcta aataagctaa aaacaccccg aatgtcttta
     9421 gacttcgggg tattatttta cctataaaaa ataaatagat gtaaaatcca tttcaaaaag
     9481 catagcccat ctcttgccac acccaataaa gaaattatca acgtttatgc tttttttaac
     9541 tcctttaagc ttgcctttgt tggtattcct caacgattct ttttgtgttc ttgtttgttt
     9601 tgtgcttttt gactttttgt tgtttaattg cgaattcctt gatttctttt ttcctgaatc
     9661 gcttcgcatc ccgcggttat gcgtgcgatt tcatcattgc tttttctgat ctaactcgtt
     9721 tcatgtcaaa gagcttcagc tgacttagtg tccatggttg ttttgatatc ccttttcctc
     9781 cttaataaag cggtgattga tactgctatt ttttccttca cataaacata acatcccaaa
     9841 aaaatcgact atcttttttc aaatggtgta ttcaagtatt cggaggaatt agtaatgatg
     9901 caccctaaaa ataacataat tcgcttaaat tcaaggctac caaaaaacta ccaagttcta
     9961 attatctaga gtttggcagt ttctcattta cacaacatat ttttcttctt tttcgatttt
    10021 ttcatagtgc ttttgttcat catcatccgt tataactact gttacatcat tcagattata
    10081 atatactgaa aaatctcttt tcccaatttt cgaatgatca atcaataaaa agcgttcaac
    10141 agaattattc agagcgatct gttgagtttg gccctcttca aaagtcgaag tcataatttt
    10201 ctgttcattt aaagcattac agctgaaaaa tgctttatga aaattcatat cctgtagcga
    10261 tttattcgtg atttcaccaa caaagcattg agtcgtttcc cgcatctcgc cacctaatag
    10321 atagattttt cgattccctt ttcgcttatt taattcttca aaaacaggca aacagtttgt
    10381 aacaatccgc agtgaatccc cttgaatgat ttttgacatg agctcgattg tagttcctgg
    10441 tccaagaaat atcgtatcac catcattgat catttcattc gccttttcca caatccgctg
    10501 cttttcttct tgcagcataa tctgtttttc tctatgagaa agctccacat gccgataaaa
    10561 atccttcaat ttcgctccgc catggacgcg ttctaataat ccttgtttct ccatttcgtc
    10621 caaatcacgt cggactgtca tatccgaaac cgaaaacttc tccataatat cgctgactcg
    10681 tactgtaccg ttaacctcta ataacgacat gatcgcttgg aatcgatccg tttttaacat
    10741 ttcatcgcct cttttctttt taagactatc aaattcttaa gaaaagtgct agaacaagtt
    10801 ttcttaagaa gccttctgca ggaaggaaag caaaattttt cagaatttgc aagctgctat
    10861 tctaaaaaat aaaaccgaac gagcatgact gtcatagtga cagccttgtt cattcgactt
    10921 ttatctttaa taaatcattt tatttaatct gaagttattg tgcaagttca aaaacaaagt
    10981 agttaatgtc tcctcgatat ttcgaaatcg aatactcaac gggcagacgt tctagcgaaa
    11041 aaccctgtgt atggaaatag aacattggat ctccttcttc cacatctaac aaatcgctga
    11101 tcgtatcatc cgctttgatt acttctagtt ttctggtgac cgttttaatt ggataaccca
    11161 tttcttcaaa aacttgatac attttttctt ttctgatttg tcaaattaag ctagacaata
    11221 tgtctttatc tatgcagctc aaaaatactt ccaatggtgt tcgataattt aatgattttc
    11281 ttggaatatg gtttcttttc gaggcaacgg attgaataaa ggtttcatcc atgtccctaa
    11341 agtccattgt tttaggtaag ccatctcgtc taagcaagcc gtttgagtgc tcatttagcc
    11401 ctctttgcga aggtgttcct ggatctgcga aaaagatggc aatatcgttc gagttactta
    11461 tagatcgcca gttggaaaat tcttttccac aatcaaatgt catggattta aacaagtgtt
    11521 taggcatgga tttgaaccaa tcatttaacc tgttttcgat atcttcggct cgtctgcctg
    11581 tcggtttcaa agtaataata accttggata aacactcaac caatgtaata acagcgctct
    11641 tgtgt
//
LOCUS       DACCVU010000091        11616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCVU010000091 DACCVU010000000
VERSION     DACCVU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.091"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..305
                     /locus_tag="IVW29_002129"
     CDS             6..305
                     /locus_tag="IVW29_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839603.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVW29_002130"
     CDS             378..1739
                     /locus_tag="IVW29_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7839604.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVW29_002131"
     CDS             1761..2021
                     /locus_tag="IVW29_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839605.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVW29_002132"
     CDS             2035..2787
                     /locus_tag="IVW29_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7839606.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVW29_002133"
     CDS             complement(2881..3486)
                     /locus_tag="IVW29_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7839607.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVW29_002134"
     CDS             complement(3539..4513)
                     /locus_tag="IVW29_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7839608.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVW29_002135"
     CDS             complement(4891..5670)
                     /locus_tag="IVW29_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7839609.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW29_002136"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW29_002136"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7839610.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVW29_002137"
     CDS             7161..8558
                     /locus_tag="IVW29_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7839611.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVW29_002138"
     CDS             8639..9079
                     /locus_tag="IVW29_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7839612.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVW29_002139"
     CDS             9227..9553
                     /locus_tag="IVW29_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7839613.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVW29_002140"
     CDS             9554..10303
                     /locus_tag="IVW29_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839614.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVW29_002141"
     CDS             complement(10383..10838)
                     /locus_tag="IVW29_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7839615.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVW29_002142"
     CDS             complement(11265..11540)
                     /locus_tag="IVW29_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839616.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3669 a         2015 c         2360 g         3572 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactga
//
LOCUS       DACCVU010000092        11586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCVU010000092 DACCVU010000000
VERSION     DACCVU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.092"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            391..597
                     /locus_tag="IVW29_002143"
     CDS             391..597
                     /locus_tag="IVW29_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7839617.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(799..1800)
                     /locus_tag="IVW29_002144"
     CDS             complement(799..1800)
                     /locus_tag="IVW29_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7839618.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1805..3718)
                     /locus_tag="IVW29_002145"
     CDS             complement(1805..3718)
                     /locus_tag="IVW29_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7839619.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3886..4392)
                     /locus_tag="IVW29_002146"
     CDS             complement(3886..4392)
                     /locus_tag="IVW29_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7839620.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4552..4992
                     /locus_tag="IVW29_002147"
     CDS             4552..4992
                     /locus_tag="IVW29_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7839621.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5018..6175)
                     /locus_tag="IVW29_002148"
     CDS             complement(5018..6175)
                     /locus_tag="IVW29_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839622.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6178..6534)
                     /locus_tag="IVW29_002149"
     CDS             complement(6178..6534)
                     /locus_tag="IVW29_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839623.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6832..7806)
                     /gene="sgrA"
                     /locus_tag="IVW29_002150"
     CDS             complement(6832..7806)
                     /gene="sgrA"
                     /locus_tag="IVW29_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7839624.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8002..8841)
                     /locus_tag="IVW29_002151"
     CDS             complement(8002..8841)
                     /locus_tag="IVW29_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7839625.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9026..9913
                     /locus_tag="IVW29_002152"
     CDS             9026..9913
                     /locus_tag="IVW29_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7839626.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10507..11202)
                     /locus_tag="IVW29_002153"
     CDS             complement(10507..11202)
                     /locus_tag="IVW29_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7839627.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11186..11584)
                     /locus_tag="IVW29_002154"
     CDS             complement(11186..11584)
                     /locus_tag="IVW29_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7839628.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3458 a         2304 c         1735 g         4089 t
ORIGIN      
        1 aaaaattaaa aaaaattttg gtgaattaag tatttccttg ataacactgg cttttggcct
       61 cattttaggt tatcatttgt aattttattt cacagttgat atgattgtta tgtaaaataa
      121 aaaagtttag gatacatcga atttttgggg aaattctttt gggggtatcc taaatctttt
      181 ctattatatt cccatatttt ctattattgg tctagacgta tgatggtaaa ctttgtacct
      241 atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa gttcgctaga
      301 tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta atctatttat
      361 catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt
      421 ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac
      481 aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt
      541 gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt
      601 ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata
      661 aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg
      721 aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct
      781 cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg
      841 cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc
      901 ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat
      961 ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc
     1021 cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc
     1081 aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac ttacttgagt
     1141 aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc
     1201 caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata
     1261 ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc
     1321 ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt
     1381 aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc
     1441 tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc
     1501 tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc
     1561 catattatta tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc
     1621 gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa
     1681 ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt
     1741 gactaaaata actacgatga taactggtac tgcaatcact attcgcttga accatttcat
     1801 tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc
     1861 cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt
     1921 ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg
     1981 attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct
     2041 tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat
     2101 taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt
     2161 gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga
     2221 gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt
     2281 tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat
     2341 gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca tataattttt
     2401 gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg
     2461 aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa
     2521 aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt
     2581 ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac
     2641 gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag
     2701 gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta
     2761 aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg
     2821 actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa
     2881 caaataataa attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt
     2941 tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga
     3001 cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa
     3061 gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac
     3121 ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag
     3181 tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag
     3241 tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg
     3301 tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga
     3361 tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt
     3421 ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt
     3481 cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg
     3541 aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag
     3601 attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta
     3661 cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc
     3721 ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa
     3781 tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat
     3841 ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa
     3901 tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat
     3961 aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc
     4021 atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat
     4081 cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc
     4141 atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca
     4201 tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc
     4261 aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg
     4321 tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc
     4381 tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta
     4441 gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg
     4501 actatttctt catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt
     4561 aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc
     4621 catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt
     4681 atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt
     4741 agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag
     4801 attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt
     4861 ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa
     4921 gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt
     4981 gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt
     5041 ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc
     5101 aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg
     5161 cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac
     5221 tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg
     5281 ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat
     5341 ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa
     5401 gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg
     5461 gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt
     5521 ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt
     5581 tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt
     5641 caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat
     5701 aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat
     5761 tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt
     5821 ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc
     5881 cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac
     5941 tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac
     6001 ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt
     6061 atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat
     6121 gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta
     6181 tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag
     6241 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
     6301 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
     6361 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
     6421 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
     6481 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
     6541 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
     6601 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
     6661 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
     6721 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
     6781 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
     6841 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
     6901 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
     6961 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
     7021 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
     7081 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
     7141 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     7201 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     7261 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     7321 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     7381 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     7441 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     7501 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     7561 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     7621 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     7681 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     7741 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     7801 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     7861 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     7921 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     7981 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     8041 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     8101 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     8161 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     8221 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     8281 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     8341 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     8401 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     8461 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     8521 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     8581 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     8641 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     8701 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     8761 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     8821 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     8881 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     8941 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     9001 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     9061 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     9121 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     9181 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     9241 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     9301 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     9361 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     9421 taaagatcta gacactgcct ggaaagaatt ctatccagaa gttagtacac agatcattct
     9481 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     9541 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     9601 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     9661 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     9721 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     9781 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     9841 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     9901 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     9961 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
    10021 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
    10081 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
    10141 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
    10201 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
    10261 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
    10321 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
    10381 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
    10441 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
    10501 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
    10561 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
    10621 ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt
    10681 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
    10741 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
    10801 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
    10861 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
    10921 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
    10981 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
    11041 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
    11101 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
    11161 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
    11221 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
    11281 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
    11341 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
    11401 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
    11461 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
    11521 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
    11581 tcatct
//
LOCUS       DACCVU010000093        11530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCVU010000093 DACCVU010000000
VERSION     DACCVU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.093"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            512..1069
                     /locus_tag="IVW29_002155"
                     /pseudo
     CDS             512..1069
                     /locus_tag="IVW29_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="IVW29_002156"
     CDS             1127..3166
                     /locus_tag="IVW29_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7839629.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEYPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="IVW29_002157"
     CDS             complement(3275..4426)
                     /locus_tag="IVW29_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7839630.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="IVW29_002158"
     CDS             complement(4580..5110)
                     /locus_tag="IVW29_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839631.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="IVW29_002159"
     CDS             complement(5209..5559)
                     /locus_tag="IVW29_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7839632.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="IVW29_002160"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="IVW29_002160"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7839633.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="IVW29_002161"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="IVW29_002161"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7839634.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="IVW29_002162"
     CDS             complement(7962..8624)
                     /locus_tag="IVW29_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7839635.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="IVW29_002163"
     CDS             8808..10454
                     /locus_tag="IVW29_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7839636.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="IVW29_002164"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="IVW29_002164"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7839637.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         3882 a         2156 c         2174 g         3318 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 tcaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaatat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aataattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg ttggctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gaacacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt tgaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgagattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggagta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    10921 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga
//
LOCUS       DACCVU010000094        11444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCVU010000094 DACCVU010000000
VERSION     DACCVU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.094"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            185..1183
                     /locus_tag="IVW29_002165"
     CDS             185..1183
                     /locus_tag="IVW29_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7839638.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVW29_002166"
     CDS             1295..1804
                     /locus_tag="IVW29_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7839639.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVW29_002167"
     CDS             complement(1834..2493)
                     /locus_tag="IVW29_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7839640.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVW29_002168"
     CDS             complement(2495..3259)
                     /locus_tag="IVW29_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7839641.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVW29_002169"
     CDS             complement(3267..3944)
                     /locus_tag="IVW29_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7839642.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVW29_002170"
     CDS             complement(3981..5366)
                     /locus_tag="IVW29_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7839643.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVW29_002171"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVW29_002171"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW29_002172"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW29_002172"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7839644.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVW29_002173"
     CDS             6305..7162
                     /locus_tag="IVW29_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7839645.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVW29_002174"
     CDS             complement(7243..7578)
                     /locus_tag="IVW29_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839646.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVW29_002175"
     CDS             complement(7645..9015)
                     /locus_tag="IVW29_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7839647.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVW29_002176"
     CDS             complement(9093..9629)
                     /locus_tag="IVW29_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7839648.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IVW29_002177"
     CDS             9938..11419
                     /locus_tag="IVW29_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7839649.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2300 c         1855 g         3772 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttat
//
LOCUS       DACCVU010000095        11424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCVU010000095 DACCVU010000000
VERSION     DACCVU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.095"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            93..1064
                     /locus_tag="IVW29_002178"
     CDS             93..1064
                     /locus_tag="IVW29_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7839650.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1142..1378)
                     /locus_tag="IVW29_002179"
     CDS             complement(1142..1378)
                     /locus_tag="IVW29_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7839651.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1347..1478)
                     /locus_tag="IVW29_002180"
     CDS             complement(1347..1478)
                     /locus_tag="IVW29_002180"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7839652.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1554..1937)
                     /locus_tag="IVW29_002181"
     CDS             complement(1554..1937)
                     /locus_tag="IVW29_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7839653.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(1970..2755)
                     /locus_tag="IVW29_002182"
     CDS             complement(1970..2755)
                     /locus_tag="IVW29_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7839654.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2933..4033
                     /locus_tag="IVW29_002183"
     CDS             2933..4033
                     /locus_tag="IVW29_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7839655.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4023..4847
                     /locus_tag="IVW29_002184"
     CDS             4023..4847
                     /locus_tag="IVW29_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7839656.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4853..5659
                     /locus_tag="IVW29_002185"
     CDS             4853..5659
                     /locus_tag="IVW29_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7839657.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5656..6486
                     /locus_tag="IVW29_002186"
     CDS             5656..6486
                     /locus_tag="IVW29_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839658.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6493..7524
                     /locus_tag="IVW29_002187"
     CDS             6493..7524
                     /locus_tag="IVW29_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839659.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7631..8904)
                     /locus_tag="IVW29_002188"
                     /pseudo
     CDS             complement(7631..8904)
                     /locus_tag="IVW29_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9131..9316
                     /locus_tag="IVW29_002189"
     CDS             9131..9316
                     /locus_tag="IVW29_002189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839660.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9440..10156
                     /locus_tag="IVW29_002190"
     CDS             9440..10156
                     /locus_tag="IVW29_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7839661.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10058..10578)
                     /locus_tag="IVW29_002191"
                     /pseudo
     CDS             complement(10058..10578)
                     /locus_tag="IVW29_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10866..>11424
                     /locus_tag="IVW29_002192"
     CDS             10866..>11424
                     /locus_tag="IVW29_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7839662.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDR"
BASE COUNT         3253 a         2136 c         2638 g         3397 t
ORIGIN      
        1 ctagtatatt tatatgagct aattttatga ttaaatggta agtttttcct tgtgtgtttt
       61 agtttaaact agtgtgtaat tcaaaaatct atatggaagg aacagtatta agaatagaac
      121 agggttctct acatgatggt gctggtttac ggaccgttgt ttatttaaag ggctgcccat
      181 tacgctgtgc ctggtgttcg attcccgaat cacaaagcaa gcagattgaa aaaggatttg
      241 gccagaccat gaccgctgaa gaggtaatgg atgagattga gaaggatgct gttttttact
      301 ttcactcgga tggcggagtg accattagcg gaggcgaggc actggttcag gcggattttg
      361 ctaaagaaat tttgcaaaaa tcgaaataca ttggaatcaa taccgtactg gaaaccagct
      421 tttgtggtgc ttataatgaa atccaaaagg tagctcccta tgtggataca ttgtttgtgg
      481 atgtaaaaat gtttaccagc aagctccaca aacaatggac cggcttagat aaccaacaaa
      541 ttttgaagaa tattcgtcgc tttttaattg agtatcctaa ctgcgaagtg cgaattcgag
      601 ttcctgtggt tccaggtatc aatatgaacc tgaccgagct gctgacgatt gcttgttttg
      661 tggcagatct tgatcggttc gtaccgttag aattactacc gtaccactgc tatggtatgc
      721 acggttacca agcattggga ctggagtatc cgttggcaga cacgccagcg ccttcaccta
      781 aagaaatgtt tgcattggca gatcaactgg ctcgcacggt tcctcatttg ctggtcacag
      841 ctttaaccaa aacatttatt tatttattga atccaagctt tatcttatgg ataaatgaaa
      901 gacactcact agctgcagcc agctcaagaa tagagctttt gctgaagtca gtgagtgtct
      961 ttttacgatg ttttttcgaa gttaatttgt cgaatgaaca ctggctcaat cggtacatct
     1021 tcactgtttg tcggctgagc tgcgatcgct gctacgattt ctaacccttg gtatacctgt
     1081 ccaaaaacag tataacgacc atccagctcc ggtagtcctc caagctccaa gtatgcttgc
     1141 tttacattag caggtcagcc tgcgcctttg tgttttgcac aataaaaaac tggttgccgt
     1201 tggtgtgggg gccgtggtcc cctaaggcca aggcgccatt aaaatgataa agtccgcgat
     1261 gcacttcatc agcgaaaaag ccctcatagc tggaaacagc ttcatcacca gaaccatcca
     1321 aagcgccacc ttgaataaca aattccttaa tgacacgagc cgaaaggtgt gttgtcataa
     1381 acgccttgct cagctaggga gcgccagttg gctacagcca ggggagcttg gtcaggaaat
     1441 aagcggagac gaatttcacc agcatcggtg accagcacac aaacttcttc ctgctgcggt
     1501 ttttctaatt gaaagagagg aaggtttgca actgaaacgc cttgatcctg ccatcaaata
     1561 tcgaaaatcc gccactcgac cttcgcctga tcaccaaggc gagccattcc caataaaacg
     1621 gtataggcgg tttcatccgc ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag
     1681 tcatctgggg cggctacttg ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc
     1741 tgatctttaa aggctgcaaa ataagcctga actttttccc gctgcaagct gtcagccacc
     1801 aaaaaatttt ccagttgttg ttttggaacg gctgctaaca aggatcggca aaccgcctca
     1861 attttcaacg catcccaatg tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag
     1921 tcccacaggt tgctcatttt tttcaccact ttcttctatt atataggaat cacaaaatcg
     1981 gcagcggcgt caggcggcgt tcttttcctt gaatgactag caacttttct acctgacagt
     2041 ctgccaacag ctgactcatt tttttaaagc ccagctgata atcttcggca acatgagcat
     2101 ccgagcctaa ggtaaagagc tttccgccga cactttgata caaaggaacg gcataacggt
     2161 aaaggtcttc gttgccgtat ttcaagaagc tcttggcatt taactccatc gccaattctc
     2221 gttcaacaac ctttttgaaa atcgttagca gctgagcctc aaaatgctcc gcgaattctt
     2281 ccgctgtgaa ggtgaagcgg cgtaatccat aatcaaagtg agtcaaaata tctccttctg
     2341 gaaaatccgt caagacctgc aacatctgat caaaatattc cttggtagtt tgaatgggat
     2401 ctttggttaa aaccacatcg tccatataat caaagcggcc attttgatga atacttacca
     2461 gctttaaatc ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa
     2521 caacgccaat ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca
     2581 gtttgtttat ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta
     2641 aatcaaagtg atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt
     2701 tttcctctga atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga
     2761 gcctcctttt ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga
     2821 aatttacaaa ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc
     2881 tctcattaca aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat
     2941 gaatgaaaaa attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct
     3001 gctgacaggc tgcggctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt
     3061 agatacgatt atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga
     3121 tacttgggca aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca
     3181 cgtggatacg gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa
     3241 tggaaccgcc gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga
     3301 gttaacctta ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga
     3361 tgatggggat tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa
     3421 tgtgaagcaa gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt
     3481 gggggatgtg acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa
     3541 cggaggtgac gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa
     3601 aggccgtttg tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt
     3661 ggcagttgtt tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt
     3721 tgaggtaact gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa
     3781 aaatgcccct catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg
     3841 gcaaatcaat ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga
     3901 agtagctgaa aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa
     3961 tgaagcttgg aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac
     4021 ctatgcaaag tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca
     4081 cagccgtcgc ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat
     4141 tgaaggtaaa aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg
     4201 gcgttccagc ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt
     4261 ggtcgctgtt tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact
     4321 ggtacagcga gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc
     4381 aggaggagcg actgattcag catggcatct tttatagtga ggacacttac cctgattcgc
     4441 atttatttgc ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac
     4501 attcgatgaa tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag
     4561 cagcggttaa tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag
     4621 gttatcaagt cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca
     4681 gcttggctgc acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga
     4741 aggaacaaac tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg
     4801 caccgtcaga gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa
     4861 acgcttggtt ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct
     4921 ttttatggtt ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta
     4981 cagtgatatt ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc
     5041 tgtcagctcc agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa
     5101 tctatcgact aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc
     5161 tgggattccg ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat
     5221 gattttgcca tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta
     5281 tacctatttc caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa
     5341 aaaggaatgg ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa
     5401 ggtggctttg ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa
     5461 tggcatgggg acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac
     5521 gattcgaatt gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc
     5581 gttggcagtc gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg
     5641 caaggtggtg cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat
     5701 tacttgctgc cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg
     5761 cttttaccta gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt
     5821 attgctgcgg tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg
     5881 ttgatgattc ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg
     5941 gaaaagctgc tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt
     6001 cgaacgtatg ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt
     6061 acggcgttgc cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag
     6121 gaattagtgg atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg
     6181 ctgtttccaa acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt
     6241 tttggggagt acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc
     6301 tatatgctgc ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat
     6361 ttgctgttta tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa
     6421 cagctgtccc ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag
     6481 ttgtgacgga aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag
     6541 attttagcta ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc
     6601 agcgggtgcg gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc
     6661 ggtgagattg tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc
     6721 ggctttgttt ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc
     6781 tttggtttga aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg
     6841 ctatcattgg tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt
     6901 caacgacaac gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg
     6961 gatgagccat taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc
     7021 gctatccagc aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg
     7081 atgcgaatca gtgatcggat ttttgtgatg gaagctgggc gggtggctca ggtttccacc
     7141 tcgaaggaat tgtatcagaa tcctcagagc cgatttgttg cggaatttat cggcaactat
     7201 aatcgtttcg aatggtatga gctgaatcag tatacccacg agttttccgc taagggcaat
     7261 cgctgcattt attatttaag accggagctg attcaatttg aaccggttcc tcagggggtt
     7321 aagattccgg ttctatggaa ggaatcattt attttaggca acattcaacg gtatgttttt
     7381 caaacgctgg agggcaaaga aattttagtg gatcggttga acgatcggga agagaaaatt
     7441 agcgatgcct tatacattca gctgaaagat attctagcaa tccctgtgga aattgatact
     7501 cagcattttg cacaacaaag ctagtgtata gtcggcaaac atttctggct ctttgtcaaa
     7561 taggactgat agagtgatat aagaacatct aaggtagaat tgattttctg ctttagatgt
     7621 ttttttatgt ttaataagtg atcagatgat tgattaagaa aattctggtt ctcatcctaa
     7681 cccgtaagct attcgtttca atgctttgat gtgggtatgc agattttcta ttttgccatt
     7741 tgagtaaggc aactctaagg ctaaacgaat gccggattca tagtttataa acgctttttt
     7801 tatatgatgc agttctcttg gaaccgactt gggcattgtt tttacaagct gaaagaagat
     7861 gtccgcttct ttgtcataaa aagcttgaag aatttcttga tagtaagcat aagctattcg
     7921 tagttcatca gaagtagaaa gtagacgttc tacgacttca ctttcggtta aataggccca
     7981 accaaaatta cgccattttg tccatttatc ataaacaagc gtgtcctgtc gtttgatcag
     8041 aagtcgagat aaagccttca cttgtcgata gtgtttggct tgtattggat cgtttcggtc
     8101 gaagcttttc atgactcata gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat
     8161 gtgaaagcga tccacaacga gtttggcatg aggaaagcac tcttttatca gcaaagcata
     8221 agaggcgttc atatctgtta cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg
     8281 cataaaataa gaaatgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc
     8341 agtatcgcca tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa
     8401 ttcatctacc aatgaacagc gaggcaacca atttattttc acgtttgtca tggggagcag
     8461 agaagcgagt gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt
     8521 cattgcttgt ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa
     8581 acagtttttt tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag
     8641 gtaccgttgc tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac
     8701 cagcgatttt ttctttccgt tcttaacgat cagcccatgt tgattcacct gtttacactg
     8761 aggacagggc tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg
     8821 tatttttttc ttctcgacat gagtgatttg aatgtttcca tctttgattc ctagtaatag
     8881 tttgataaga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
     8941 ttctaacgga gcttctgtat gttttgtttt tttatgctca aaaggactga tagaatgatt
     9001 tactatcagt cctagaaatt atagcgccac atttctttca cctttgactt ttcagacggc
     9061 agtttttatg ctgatactgt agagagagca ggacacagct tttgcttgtt ttgggtaagg
     9121 gggaaagaag atgaccaaag cacttatttt tgattttgat gggacgatga ttgacagtga
     9181 atggatgttt tacgagagta ttcatcacta tttactgaag cctatttact ggagcagttc
     9241 ggctataaaa ttccttttgc agactatcaa caaggtgttg ggacggcagc agaactgtcc
     9301 ttttatgaag ggataagtca agcggttggg agaccagtgg atgctgaaca ttttgagcag
     9361 cggatcagca gcactggaaa atcggagtag tctcaattct gaactgcggg agctagacta
     9421 tttttttgaa gcacatccga tgattaagga aaaaattgat tcctttgtga cgatcgatga
     9481 tcttcaggtc ggcaaaccag atccagaagg ttatttgttg tgcttgaaac ggctgagggt
     9541 tgaacaggct gttgtagttg aggatagtcc agtcggtgcc caagcagcac cgcatatgac
     9601 agttgatgta caagcgttgg atgcagattt tttcgccttt agcgggcata aaatgtgcgg
     9661 gccgacagga atcggtgtat tatatggcaa acgtcaattg ttagaacaaa tggagccagt
     9721 cgaattcggc ggtgaaatga tagattttgt caatctttac gatagttcat ggaaagagct
     9781 gccatggaaa tttgaagctg gtactccgaa cattgcagga gcaatcgatt acttgaatga
     9841 gattggtatg gagaatattc atcgttatga gcaagaactg gttgcctacg ttttacctaa
     9901 attacaagcg atcggcggta tcaccattta cggaccgcaa gatcctgaac atcatactgg
     9961 cgtggttgct tttaatttgg atggcatcca tccccgtgat gtggcgactg ctctggatat
    10021 ggaaggtgtc gctgtacgtt caggacacca ttgtgcacaa cggttcatcg acgatttctg
    10081 tttgactaat tggtcgttta aacagtcgat gataatgccg tttgagcggc tccaatgtcg
    10141 tggaatcttt gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt
    10201 cagattacgt gattgatttt cgtacagttc tatgagaatc gcctgcttca gttcctttgt
    10261 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata
    10321 gcatagaaaa cgggtacgtt ttaattctaa aatagtcaac ttttctttga ctttcaacat
    10381 ctgagtacgt gtcgtatagg aaccgttgcg gataatacga atcgatccac agtgttcaca
    10441 atgggaaggt gagtcatcca atctcccttt aataaactgc gcttgaacgc ctttatattc
    10501 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
    10561 tctagtatga ttatccatag agcgactcct ttggtagggt gatgttttgg tcgactaaat
    10621 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
    10681 ccttcgattt tccaacaaca caaaatatta tagaagcctt ttttctaaag gatttcaagg
    10741 tgtctgtttg catttattaa aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
    10801 ctagatatta tagagccata atatgctgta taatatctag tgtataggct taagtggagg
    10861 tatatatgaa aatattaact gttgaagatg ataatatgat aagagaggga ataagtgaat
    10921 atctttcaga atttggatat actgttattc aagctaaaga tggaagagaa gccttgtcaa
    10981 aatttaatag cgatataaat ttggttatct tagacattca gatacctttt ataaatggtt
    11041 tagaagtgtt gaaagaaatt agaaagaaaa gcaatttacc aattctaatc ttgactgcat
    11101 ttagtgatga agaatataaa attgatgcat ttactaattt agtagacgga tatgtagaaa
    11161 agccattttc attgccggtc ttaaaagcta gaatagattc tttaattaag aagaattatg
    11221 gacatttaga gaagtttgaa tataaggatc tatcggttaa ttttaatagc tatacagcta
    11281 aaataaatgg tgaagaaata gatgtaaatg caaaagaact agaagtatta aaatgtttat
    11341 tggataataa tggacaagtg ttaacaagaa tgcaaattat tgattatgta tggaaagata
    11401 gcgaagaaac tccttatgat cgag
//
LOCUS       DACCVU010000096        11377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCVU010000096 DACCVU010000000
VERSION     DACCVU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.096"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            339..1598
                     /gene="lysA"
                     /locus_tag="IVW29_002193"
     CDS             339..1598
                     /gene="lysA"
                     /locus_tag="IVW29_002193"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7839663.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1585..2385
                     /gene="dapF"
                     /locus_tag="IVW29_002194"
     CDS             1585..2385
                     /gene="dapF"
                     /locus_tag="IVW29_002194"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7839664.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILGE"
     gene            2388..3269
                     /locus_tag="IVW29_002195"
     CDS             2388..3269
                     /locus_tag="IVW29_002195"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7839665.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3266..3982
                     /locus_tag="IVW29_002196"
     CDS             3266..3982
                     /locus_tag="IVW29_002196"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7839666.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3988..4683
                     /gene="dapD"
                     /locus_tag="IVW29_002197"
     CDS             3988..4683
                     /gene="dapD"
                     /locus_tag="IVW29_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7839667.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4699..5700
                     /locus_tag="IVW29_002198"
     CDS             4699..5700
                     /locus_tag="IVW29_002198"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7839668.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5697..7058
                     /locus_tag="IVW29_002199"
     CDS             5697..7058
                     /locus_tag="IVW29_002199"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7839669.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            7018..8169
                     /locus_tag="IVW29_002200"
     CDS             7018..8169
                     /locus_tag="IVW29_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7839670.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8144..9313
                     /locus_tag="IVW29_002201"
     CDS             8144..9313
                     /locus_tag="IVW29_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7839671.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9488..10867
                     /locus_tag="IVW29_002202"
     CDS             9488..10867
                     /locus_tag="IVW29_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7839672.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11148..11377
                     /locus_tag="IVW29_002203"
                     /pseudo
     CDS             11148..11377
                     /locus_tag="IVW29_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3800 a         1971 c         2526 g         3080 t
ORIGIN      
        1 atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg aaatgattca
       61 taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat tttttcatca
      121 ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt cgggtatttt
      181 gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat actagacaat
      241 tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa atgaaattcc
      301 aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg ataattggcg
      361 gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg tatgatgaag
      421 aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa cgttttgata
      481 caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat ttagttcgtg
      541 aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct ttgcaagctg
      601 attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag gaactgatct
      661 atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa gttgaattat
      721 tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg
      781 gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca aaattcggca
      841 tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa agcagctatc
      901 tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt actgcttggt
      961 tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct ctttccttga
     1021 atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc ccagtcgaag
     1081 aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga aaactgacca
     1141 ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt acaacactat
     1201 acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta gatggcggga
     1261 tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat ttagcagcaa
     1321 aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg
     1381 atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt ttagctgttt
     1441 attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg acacgaccag
     1501 cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa gcattttctg
     1561 atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg cggaaatgat
     1621 tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc ttctttatgc
     1681 acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc gttggaaatg
     1741 atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt
     1801 gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt agagacatta
     1861 gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag cgtagatatg
     1921 ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac
     1981 caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt aggaacgatt
     2041 cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg
     2101 atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga agtagtcaat
     2161 gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact tgcttgcggt
     2221 acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc agcagatcag
     2281 gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc gatacaaatg
     2341 gctggcccag cagttcatga atggacaaga atattggggg aatgaagatg ataaaaggtt
     2401 cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat gctggtttgg
     2461 aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt ttaggtacaa
     2521 caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa ctgacagtaa
     2581 agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat acgaaagaaa
     2641 cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg gtcatcaccc
     2701 cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca attgcagaag
     2761 cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg tcgattccaa
     2821 tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa gaagcctcag
     2881 gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc ttcctttaca
     2941 gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg
     3001 tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg caggacggaa
     3061 gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat gccttgttca
     3121 gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta ccttccggac
     3181 ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta gctcagcttt
     3241 tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg gacagatggg
     3301 aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt tacggacggc
     3361 acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta ttgatttttc
     3421 tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac ttccgttagt
     3481 cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac atgccaaaca
     3541 tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga atcaactaat
     3601 caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag
     3661 tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa tccaagaagt
     3721 gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa accaaattgg
     3781 tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt ttgctgctac
     3841 tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg ctgcaggcgc
     3901 agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat tagaagattt
     3961 attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa
     4021 acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt
     4081 aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga ctatgagacg
     4141 attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct
     4201 gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg tatcgaacca
     4261 ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg
     4321 attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg tgctgtctta
     4381 ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt cttagcaggt
     4441 gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact ggtaggtgcg
     4501 aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc agcaggagca
     4561 gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc acgtgtatta
     4621 aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt acgcgaatta
     4681 tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc aaccggatta
     4741 gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg acgattgaaa
     4801 ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact
     4861 gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt
     4921 gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt tgtcatagat
     4981 aattcctcag catggcggga agattcggat attccattaa tcgttccaga ggtcaatctg
     5041 gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat tcaagcggta
     5101 cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta ttcgacctat
     5161 caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac
     5221 gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga aatcgatcag
     5281 cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac ccgtaagatt
     5341 ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat cgaaaatgga
     5401 catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga aattagagag
     5461 tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca taaatatccg
     5521 acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag aaaagacact
     5581 tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa aggagccgct
     5641 gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt ggcaagatga
     5701 gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa gagcaattca
     5761 aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt
     5821 taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg atttatgctt
     5881 accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat cgtttcattc
     5941 aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca cgcatacaga
     6001 aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt gaatatctga
     6061 ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa gacctgattt
     6121 tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc acaaaagcat
     6181 ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct gcaggaaaag
     6241 aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg aagatgttgc
     6301 aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct gatccgcgaa
     6361 tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga gagatggctt
     6421 atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag gctgatatcc
     6481 ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg
     6541 aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt ctttccatca
     6601 ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca atgagcattt
     6661 ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat atcggagtga
     6721 tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa ttaaaagaaa
     6781 cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct gtcgttgggg
     6841 gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg aacaaattag
     6901 aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc atcggtgtac
     6961 cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg aatacggatg
     7021 ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca tgaattcaag
     7081 acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc
     7141 gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc ttttcgaaca
     7201 gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc taagcatgaa
     7261 ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg gttgactgac
     7321 tttataacta aacacattga ggatcttatt tataatgtag tactgatcta tcagccatca
     7381 gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga taaatatgaa
     7441 gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga agttttctct
     7501 cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa aggccagtct
     7561 gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt acgatttttg
     7621 aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct aatgaaattt
     7681 ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat tgtatccgga
     7741 agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt agaccattta
     7801 gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg ctatccagct
     7861 gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact acatgaatta
     7921 gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata tccatgtgta
     7981 tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt cgattttgat
     8041 atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac agaaaatctt
     8101 cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga atttatccaa
     8161 tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat tcgatcagca
     8221 aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt ttccaactcc
     8281 tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc actataccgg
     8341 aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac agcgttatgg
     8401 attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc
     8461 ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag cgcctgctta
     8521 ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga tagatacttc
     8581 tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac aatatggatc
     8641 agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga cattgtctgc
     8701 aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg ttataagtga
     8761 tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta cttatcttcc
     8821 agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg gttggcgagt
     8881 gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc atcaatatct
     8941 ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt
     9001 gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc tggtccagca
     9061 attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact tgttttgcaa
     9121 actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag cggaacaggc
     9181 aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt atatccggat
     9241 ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca aagacttttt
     9301 aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg cggtatattc
     9361 tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata
     9421 actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat tcaaggaggt
     9481 ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag ggcatcaatc
     9541 gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat ttgaagcagg
     9601 agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca aggtaacaga
     9661 tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg ttcatatttt
     9721 aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg ttactagcgc
     9781 atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt ctggtgtcaa
     9841 accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga tgcgtggata
     9901 caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa aaatcgctgt
     9961 cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag ctgggaaaga
    10021 agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg ctgagatgac
    10081 agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa atgcaaaaat
    10141 cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa aagaaatccc
    10201 agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt taaaagggat
    10261 cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa cgaatcttcc
    10321 agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac gcaaaaaaat
    10381 gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa aacatctttt
    10441 tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat taagtgtctt
    10501 tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag caggagtaga
    10561 tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag aagaacaagg
    10621 aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat tgctaggagg
    10681 agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac tagcgatcca
    10741 aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc caggctttga
    10801 ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag aaccatttgt
    10861 agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg catcaagtaa
    10921 taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac ttattaccgc
    10981 agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac gatttgtgta
    11041 ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt ttttatggtt
    11101 aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg caaaggcgat
    11161 tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt cgggagaagc
    11221 acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt ttctctatca
    11281 ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg gcatacacgc
    11341 gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACCVU010000097        11339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCVU010000097 DACCVU010000000
VERSION     DACCVU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.097"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(366..1484)
                     /locus_tag="IVW29_002204"
     CDS             complement(366..1484)
                     /locus_tag="IVW29_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839673.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="IVW29_002205"
     CDS             1955..3262
                     /locus_tag="IVW29_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7839674.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="IVW29_002206"
     CDS             3381..4580
                     /locus_tag="IVW29_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7839675.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="IVW29_002207"
     CDS             complement(4607..5875)
                     /locus_tag="IVW29_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7839676.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="IVW29_002208"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="IVW29_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="IVW29_002209"
     CDS             complement(7092..7595)
                     /locus_tag="IVW29_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7839677.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="IVW29_002210"
     CDS             complement(7718..8482)
                     /locus_tag="IVW29_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7839678.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="IVW29_002211"
     CDS             8590..8865
                     /locus_tag="IVW29_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7839679.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="IVW29_002212"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="IVW29_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7839680.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10632)
                     /locus_tag="IVW29_002213"
     CDS             complement(10432..10632)
                     /locus_tag="IVW29_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839681.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2387 c         2002 g         3549 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaata
//
LOCUS       DACCVU010000098        11277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCVU010000098 DACCVU010000000
VERSION     DACCVU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.098"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(9..1644)
                     /locus_tag="IVW29_002214"
                     /pseudo
     CDS             complement(9..1644)
                     /locus_tag="IVW29_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2083..2952)
                     /locus_tag="IVW29_002215"
     CDS             complement(2083..2952)
                     /locus_tag="IVW29_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7839682.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2972..4093)
                     /locus_tag="IVW29_002216"
     CDS             complement(2972..4093)
                     /locus_tag="IVW29_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7839683.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4095..4490)
                     /locus_tag="IVW29_002217"
     CDS             complement(4095..4490)
                     /locus_tag="IVW29_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7839684.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4509..5306)
                     /locus_tag="IVW29_002218"
     CDS             complement(4509..5306)
                     /locus_tag="IVW29_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7839685.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5308..6096)
                     /locus_tag="IVW29_002219"
     CDS             complement(5308..6096)
                     /locus_tag="IVW29_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839686.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6110..6586)
                     /locus_tag="IVW29_002220"
     CDS             complement(6110..6586)
                     /locus_tag="IVW29_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839687.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6658..9315)
                     /locus_tag="IVW29_002221"
     CDS             complement(6658..9315)
                     /locus_tag="IVW29_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7839688.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9334..9738)
                     /locus_tag="IVW29_002222"
     CDS             complement(9334..9738)
                     /locus_tag="IVW29_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP7839689.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9970..10227)
                     /locus_tag="IVW29_002223"
     CDS             complement(9970..10227)
                     /locus_tag="IVW29_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7839690.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(10352..10684)
                     /locus_tag="IVW29_002224"
     CDS             complement(10352..10684)
                     /locus_tag="IVW29_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839691.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3599 a         2228 c         1629 g         3821 t
ORIGIN      
        1 aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa
       61 agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg
      121 gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc
      181 aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag
      241 cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg
      301 ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt
      361 gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta
      421 acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc
      481 atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt
      541 aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga
      601 gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt
      661 attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact
      721 aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa
      781 agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat
      841 caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat
      901 cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt
      961 cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc
     1021 acttactaca aagccgccta agactagtaa gccaacggct attacactca taaaagtgga
     1081 aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt
     1141 tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat
     1201 cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata
     1261 taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa
     1321 catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa
     1381 attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata
     1441 attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga
     1501 agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg
     1561 aacatttcta aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa
     1621 tattattttt ttctttactt gcattactta attatatact cctttcatgg attgctttat
     1681 ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca
     1741 ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat
     1801 tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt
     1861 gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg
     1921 gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag
     1981 gcgctgggta tcatgagtga atgatattgc tgaatacaaa tagtccgacc acattaagtt
     2041 gatcggactt cataagcatt ttattgacta atactttttt gcttaagaat tgattctaac
     2101 atcttttgct aaagttaagc atcctatagt tcctgcatta tctgctaact taggtgtgac
     2161 aatgtattct tcgatatttg gcgtatcgac atagccgtta attagttcta aaaaacgtct
     2221 ttgtactttc tttttcagat gttcttgttg catgacacca ccaccaaaaa ttataatatc
     2281 tggtgaaagc atcagggtcg tattacaggc acattgtgca atataatccg cctcgatttc
     2341 ccagaacgga tcttctatag aaagatcttg tcccttggtt cccgtacgca tctcgatagc
     2401 gggtccagat gccaaccctt cgagacaatc atgatgaaaa ggacaagtac cagagaaagt
     2461 atctcctgaa tgtctcttaa ctaacgtatg ccccatttct ggatggctaa acccttcaac
     2521 aaaaatccca tcttgtatag ctcctccacc aattccagtt ccaacagtgt aataaacaac
     2581 actggataaa ccttttccat gacctgaaac atactccccg taacaagcag cattcacatc
     2641 ggttgtccag gcaattggaa tttcgaaatg cttattcaac gttccaacaa aatcaaaatt
     2701 tttccaggca gtttttggag tagaggtgat atgtccatag gtctttgatt cacgatggat
     2761 atcaatcggt ccaaatgacc ctactccaat gctttctaat tgttctttat agtgattaaa
     2821 gaaatcaatt accaaactca ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc
     2881 taaaaccttt aaatcatcat tgccaatggc acaaacaaat ttagtcccac ctgcttctat
     2941 tgagccatac atttcttact ccaccgtcct tttatctagg aattcgttct tccctgtcta
     3001 atagtttttc gaaaaactca tgtggttcct tttggtacca ataggcaaca gaagaaatat
     3061 cgtcttgacg ctcataaagc ttgatgtcat catttcctag ggtttggaaa gtaacacgta
     3121 aattttcttt gaaactaatg gggtcgggta aatgccagcg gtacaaccca tgcatcggta
     3181 aagcatcatc gccaaaacca tgaactggat ttggattagg ttttccagca ctaaaataat
     3241 cccgggtgtg atctctttta gattgaaaag gatagcccat aaatagcgta ttaaaatttt
     3301 gagcatatgc cctttctcca aagttctttt tatggaaagc ccaagcgccg ccaaaataat
     3361 cttctgctcc tgtagatgtt attgtaggga aatcttcatc tccatctata taaaacttaa
     3421 attctccttc cccccaccag tatctttcca atgcgcacaa agcaaagtag gttcctacat
     3481 aatacccagt gcctttgatt ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg
     3541 gttcacggtt ccatttagca tgaaaataca tcgcatcatc cggtacttca gtcaatgcat
     3601 aattaaatgt ataaaaaatg tgcttaataa actcaggatg ttcattcgtt aaagtaatct
     3661 tagctttttt tcgaaatggc atctcaaaat agcaattcat ccctgatgtt ggattcacta
     3721 cgattggtaa ggaattcact tcgcatcttg tcccaaaccc attacaaaaa aaatctccta
     3781 atggactttc tacagaagga gtggattcat catcccaata aatacgcaaa attaaatcac
     3841 gtaagacgaa actcccagcg attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa
     3901 ttccagggcc ttccatttct gctaaagtaa ttgtctctcc agcatttatc ggaacagaag
     3961 gtgacccttt tctcccaatt cctagatgac tcgctgtcat tgccgctttc cctttttcac
     4021 catttttatt ttctggtgaa atagttctac tttcctctcc cttgtggagc catatattat
     4081 ttaaacaatt cattttattc gtcctctatc ctttcttcac ttttttgatt attcatatag
     4141 atgatttgtt gttgtgcctc cgcaataatt gctccaatat ctaaattatc atttcctaac
     4201 tgcaaagcga ttgacattgc caagggaaga ttcattcctg caattacgtg cactctgtca
     4261 gaaatatagg taaaaaactt ttgatttaca cttcctccag ccatgtcagt taaaacaatt
     4321 atttcgtctg tagtatccac taactcttta atttgttcat ctatatcatc aatttcttcg
     4381 acatatgcac ataagctact aatttgatta tgcatacctg ttaaaaactc tagtgtctct
     4441 ttaaaaccct cagctagtat tgaatggcta gcaatgatca atcttctcat aattcaaatc
     4501 cttttccttt acgctaatat tccaaatgca gccataacca tcgcaaaaac aataacgagt
     4561 agaatcaaaa tggtcatact gatttttttc ttactgagta gataatatgc tgcagcagta
     4621 ataattactg gaattaatgt tggtaaaatt ttgtccaaca ttggttggat tttcatacta
     4681 acttcaccaa aagtgaattt aagtggcgtt gaaacactga taactgaagc aattaatgat
     4741 ccaactactg ttagccccaa tactgaagca gcttcagtta aataattaat cttttcaccg
     4801 tattcagtaa caatcttcat tccctgctta taaccaaatt caaagcctct cattcggaaa
     4861 aagaagatta ccatgattaa aatgaaccat aaaaagattc ctacaggatt attttctaaa
     4921 gccatataag ccgcaattga tcccatgatg gttgggatca tcgtcatcaa aagagagtcc
     4981 ccaataccag caattggacc catcgtacca actttaaagt cattaatcgc atcttttgct
     5041 tctaatcctt gcttttcctc cattgcaaca caagcaccta aaataatggc tgccagccaa
     5101 ggctgtgtat tgtaatactg aaagtgatta tttaaagctt cgatgtattc atcatcatct
     5161 ttgtataatt ttcttaaaat aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt
     5221 tgataattaa aggtacaaac tgacatcaac caacgatagt tcgctttcct aagatccttt
     5281 ttcgttactt gatatttttc gctcatttta ttcatcctcc ccaataaacc caacatcaga
     5341 agctcccgca gaagcagtga tttgtgcatc tttttcagct ctatgataca cattaaatgc
     5401 taaagcgaat cctatgatcg ccgctccaat aataggtacc ttcaaaaaag cggctaagaa
     5461 aaatccaaca agtaaataac taatgtatcg acctgtaggc ataaagcgta acaacatcgc
     5521 aataccaaca gcaggcaaca tttttccggc taatgttagc ccatccataa accaattagg
     5581 taaaacatcc aaaacgtaat taacagcaga ctgtcctaaa gtaacggcaa taaatactgg
     5641 aattgctgca gataaaccaa agaaaattgg ccccaaccac aaaacactat tcattttctt
     5701 aaattctcgt ttattcgcat attcctgtga tttgcgaaca acaaaaccat ttaagatttt
     5761 gacaattaca tctagttgca cccctaacat acctacaggt agtcctattg ctaatccggc
     5821 ttctactcca agctggctac caaatgccgt tgcaataatt gcgcacgttc catagtccgg
     5881 tacagatgag ccaccaaaac cggcaactcc taatgacatc aattgaactg taccgccaac
     5941 tatcaaacct gtttttatat cacccatcaa aattcctgtg aataatcccc aaaaaactgc
     6001 gtaatttaca aagatcatcg gaatcccata ataatcaaca tgtttaataa atgctaatat
     6061 agtcagcata ataatttgaa aaatagtaaa atgcattatc cttactcctt tatcctaatt
     6121 ttgattgaat ttcctgtgcc ttatccgccg gaacaaattg tgtaacaatt tctacaccag
     6181 aatccaacaa ttttttatac gtgctgatat tttcatctga tgcataggct ctagaagata
     6241 gctgtaatgc attatcttta acaacagtac cgccaacaat tagtcggtca attctaatac
     6301 cgttatctac taaatgaagg attgtttccg gctctttcac gatgacaaat actttttcct
     6361 gatcgtattt ccccttttta aaattcgcaa tcgctgtttc ttcagtgata attgatacag
     6421 ccattccagc aggtctagct aaacgcatac ttgatttttg gatgtcgtta tttgcgacag
     6481 aatcatcgat caccatcact ctgtttgctt tagacattgg tgcccattgc gtgacaataa
     6541 tcccatgcaa taaacggtta tcaactctcg acataacaat actcataaat acttcctcct
     6601 ttatctttca ttttcttaca agcaattacc atgccaaaac aaaaagaact tgataaatca
     6661 agctcttttt acataatcat aaacgtattt aacctcatga atcgggatgt cgacactata
     6721 ataatgttca acgacactga aagattgctt aaaatgtgtc tgaaattttt gaaattcctc
     6781 actcgtttca ttgaaataaa tttcatcttc caaagtattt tgttttacta atcgttcaat
     6841 taagcaacta atgtgaacat ataatccgaa acacgtatta ttatcaaatg aaattcctag
     6901 atcattttgt aagatgtata tagcatctga aacccgttct aataatttag tcggatttaa
     6961 tatggtcaat tgctccatta aattcgttag actaaaattc ttcagaatat tattgttcaa
     7021 aactttcagt tcattatcat taaagtgtct attcaattga ttggagaatt tacttatatt
     7081 cattcctaaa atcatatctt caattgaaaa gaattctaca tcttctagat gtggatttag
     7141 tgttccaaca ataaaaagaa tattgttctc tttacgaagc tccgataaat agttttcatt
     7201 cactaaagag taatagtcac acgtttctat gacaagatca gtatcccttg gaaagctatc
     7261 ttcaagtatc ctttttaatt taaacgaagt tcctaatccg cttgcacaag aacagacaat
     7321 catatccttc ttaatatttg atgattcata aatataatcc acattttgat tttcttttac
     7381 ttgttcaaga atctgtttaa gcggtaaatc taataatatc tgatttgcaa catcaatagc
     7441 cattcttgtt gaaacattgt ttagtagtcc gaattttttt tgagcgtcta gttccaaatt
     7501 tagataaatt ttctccagtg aacccatatc aacaagtaaa ataatttctt catatccggt
     7561 acactcttgt aagtatctat taattttgtc tgcaatctca ctacttgaag tcattagtgg
     7621 catatcaatg gcgtcgtata tatatctctc aacgatccta ttagcagtac ttgctataca
     7681 ggttgccgtt agatctccat gagccaaaat cagtgctaaa cgtttttgct gaaattcttt
     7741 actacacatt aacttaaata aaataaccaa aacatttttt tgaatttttg ttaaataaat
     7801 atcccctatt tgaataaaac gattgactaa ttgaacttct cgactacacc aactattaat
     7861 atcattgatg aaggaatata atttatcgtc cgatgttaat tggatttgat aaatccaaga
     7921 attcaaaaag atagacgtta tttgaagata ataatagtca aattctattt gaaaatctct
     7981 ctctatttcc ttaacatact gtctaaatcg aatctcttga taggtgggat atgttaaagc
     8041 tgaattattt tggatatcaa cattcttttg tctaaaatat ctatcatatt tctcaaaaag
     8101 ctgctgctta tcagtgtctt tcttccacgt ttgataaaga tactctataa gttcgttgat
     8161 attaaaaact tcgtctactt tttccaaatc atccatttga atatatttta attcattctg
     8221 tcttttcata accttcatgc cgttgaaatt cacccattgt ggtaaatgat acatctgaat
     8281 ataaagttct tccaaatcat ctaagaaata aaaggcattg gcacaggtca attgaatgat
     8341 atttttcaaa ttgccaacat tttcactaaa gcgatagttt aataaaaaat ctaataattg
     8401 ttgagatatc agtattttct ttgctagtct ttcttcttct gatttaaaaa atatcttgat
     8461 taactgtttt ctttcataaa gagaacgact gtctaatgtt ggaagtgtta gctttacagg
     8521 tattcttctc aacaaagttg ataatagttt atcctcaggt ggttctgatg ttgcaaatat
     8581 aatccagcaa cttgatgtgt accagttttc attatcaccc atccgatgaa atttcctcgt
     8641 atccataaac tgaaaaagtt tctcctgaca ttcattgctc aatgcatgaa tttcatctaa
     8701 aaataagact ccattattag ctaattctgc taaacccttt cttccatctt cggctcctgt
     8761 atatgcacct tttacatgcc caaaaaaatt ggacaagaaa agttctggat tattggcata
     8821 ttcactacaa ttaactgaaa tgaaagatgc cttctcttct atataattat tggccaacaa
     8881 atccttatac acataatttg ctaaatagct ttttccagtt ccagtctcgc catgaagtaa
     8941 aatgggtaag ccatttggcg gatagtagat tgctgcttta atttgagcaa ttacatttcg
     9001 cagacttcct tgacttccga taaccctttc tattccaacc ctaaaagagc tatcatacca
     9061 tttttccgct tcctctgaag agctaaaaat ttgttgattt ggaactttct ccggtaaaga
     9121 aaagaagatt actggacgag tattgatttt tccaaattct ccatctttta cgaattcatt
     9181 caaatattga gagacaatat tacgactaac atttaatttt tcagatattt tctgtgctgt
     9241 attgttcccc catttatttt taatctcaga gttctctatg acataattga caatgcgttc
     9301 ttttaattct tccatcacag cacctcctcg acactattcg aattttgccg acagtatttt
     9361 tccataaata tgacttgcta aaaaggcttt gtttagatca ttcaattgga acgttgcttg
     9421 ttttgcttga tccgctaaga cgttgaaaag atactctggc gaaatcgcat accccaaaat
     9481 atcaacgatt gttgcaatca gatcttcttt tgattcttca tctgaaagta attcacgata
     9541 caacatcgtt tgtttgctta ctgtgtcata ttcctctaag gattcatctg ctaaaagaac
     9601 cacttgttcc aacaaacggt cggataaata tttatagaaa atcagcccca ataaataatt
     9661 tttatattct gacgcatcca ttttactacg gagattatct gccgcactaa ataacttctg
     9721 atttaattct gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc
     9781 tcttatttta gcatactcag ttttagaaaa agcaaaaaaa taaagaaact tattcttgat
     9841 aacaagctac gtgacgtata tccctaacac aaaaatagcc atatcctttg gtttggatac
     9901 ggctatttga tattactcat tcaattatca aatcacagta ggtgtgaatc tcatcgggat
     9961 cggcaatatc tacttctgat taggatcaaa ggtgattttc tcaataaagg tgtgatacaa
    10021 agctttaact tgcttcttct cactctggat aagaaagcaa tccaaactag tcaatatctg
    10081 ttggacatct ttgacttgaa tcggttcccc ttgttgttga aagatcccta agatttcatt
    10141 ctctcgaatt tgtaactgtc ttcgtttttc agtcaattca tttaaacgat cttttaacat
    10201 cggaaacaat tcgggactat cttccaaagc agcttcctgt ttttgctcta atagttagat
    10261 tttgcgtacc attttcttct tttattttcc ttcgattgct gattttaata tagtctaaag
    10321 gaaatttata tttattgtat tctttataac ttcaagagaa cttcgtttga gtattactgt
    10381 atttttttaa ttccataaga aaattagtta ttacctgcca cctaatcagc aaatctgaac
    10441 ctgagtcaat ataattaata ttattttcca tctccaacat tgtaagtact gttggtgtta
    10501 atatagggac tttgtacaat tgtatttttt tgattttatc atctgcacaa aggagatttt
    10561 ttaaatctaa tccttctttt atttcaaaag ttgaattttt taaatctata agagtaaaac
    10621 caattccttc atactgaatt tctgaaaatc gaatttttct cccagttgat agatcaaggg
    10681 gcagaaaacc ttttattaca ttgtgagtgt caccatcatt tacaatgtca ttaggaattt
    10741 tgattttaga attctgtgga gcacctattg gaagataata attcattata attttgtagt
    10801 cttcatgagg aaataaagta cttttaggat tgctaagata ttctcgtacc gaatctaaga
    10861 atggcttatc cattgaatct tcatccccat ttaaactaaa aaacattcct acaattgctt
    10921 ttgcaactct aagtgaattc attttcttta ttgaatgtgt taaatcagac ttaccattct
    10981 ttaaacttgt attgttataa aggaaatttt tattgtcttc tgcaaatcta atatattcct
    11041 caacatactc actaccaaaa aaactattac atttactaca taacgtattg gaatggcaac
    11101 agtttttttg acaaatttta taaggtgcag aacttctttc cgtatgctat tccgattgtc
    11161 cttgacaatg agcctcctcg gatgtgacga tcttcggcag gagctaacag ttaaagttag
    11221 actgcctcgc tagcgttagc acatccgagc tcattatcaa ggattcgctg cgccaat
//
LOCUS       DACCVU010000099        11035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCVU010000099 DACCVU010000000
VERSION     DACCVU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.099"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(79..1887)
                     /gene="uidA"
                     /locus_tag="IVW29_002225"
     CDS             complement(79..1887)
                     /gene="uidA"
                     /locus_tag="IVW29_002225"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP7839692.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1910..3676)
                     /locus_tag="IVW29_002226"
     CDS             complement(1910..3676)
                     /locus_tag="IVW29_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP7839693.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3689..4483)
                     /locus_tag="IVW29_002227"
     CDS             complement(3689..4483)
                     /locus_tag="IVW29_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7839694.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4493..5869)
                     /locus_tag="IVW29_002228"
     CDS             complement(4493..5869)
                     /locus_tag="IVW29_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839695.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5989..6636)
                     /gene="eda"
                     /locus_tag="IVW29_002229"
     CDS             complement(5989..6636)
                     /gene="eda"
                     /locus_tag="IVW29_002229"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7839696.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6720..8147)
                     /gene="uxaC"
                     /locus_tag="IVW29_002230"
     CDS             complement(6720..8147)
                     /gene="uxaC"
                     /locus_tag="IVW29_002230"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7839697.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8221..9300)
                     /gene="uxuA"
                     /locus_tag="IVW29_002231"
     CDS             complement(8221..9300)
                     /gene="uxuA"
                     /locus_tag="IVW29_002231"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7839698.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9303..10934)
                     /locus_tag="IVW29_002232"
     CDS             complement(9303..10934)
                     /locus_tag="IVW29_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7839699.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         3501 a         2252 c         1417 g         3865 t
ORIGIN      
        1 gaaaatcttt ctataaaaaa caaaaaaaaa cgattaggct aggctaatcg ttttttgaac
       61 attcttaaaa aatccaaatt attttttata actgaaattg ggtattgatg accatcgttt
      121 ctgtagatag taagcaatca ttttaggttt tctatctcta gtaaagattc ctttcttatt
      181 tccttgcact cttaaaatcc cttgacttgt tgcaaaatct gcaaaattcc acacttgttc
      241 accaacaaaa ttttcacatg tatctattac ttcgtgattt gcttcgtaat aagccacttg
      301 atactcttct gtaaacattg ttggtactgt atcatgaaat ccagctactg tatctgctcc
      361 atattctgta aacattattg gcgtatcagg acatctttcc atccatccat ctagctcttt
      421 tttcaacaga tattttgcat gtgccagatc ccctcccgaa acataccaac cataataacg
      481 atttaaacat aaaacatcga caatatctcc taccttacaa acatctggcg ttgattttag
      541 ataagtaacg attgttacag gtcttttctg cggatcaagt tctttagtta attcaactaa
      601 aggttcaaaa tattctttag caccttcgga atccgaatct ggttcatttg caatagacca
      661 cattacaaca cttgggtggt tcttgtcgcg ttctatcaat tccttgatca ccgttttatg
      721 agaattttct gttcgtatct ctttccacgt attatgtttt tccacattat catctaataa
      781 agtggccatg aaatttaaat gtaaccctac tgcaggtact tcatcaataa ctacaatacc
      841 ttctcgatct gctaatcgca taatttcttc tgagtaagga taatgtgatg ttctaaaaga
      901 attagcacct atccatttca ttaaattaaa atctttaata tttacagcct cattcaaacc
      961 acgtccatta acatacgaat cctcatgctt accaaatcct ttaaaataaa aaggtttatt
     1021 attaatcaaa aatttgccat tggtaacctg aacactgcga ataccaaatt cttctgtata
     1081 ggtatcgatc actttattat catcaattaa actaattttc atactatata aataagcgtc
     1141 tagaggttcc caaaaaattg gattcttaag ctgaatcatt cctttctttc cttcagcttc
     1201 agctacaagt tgagcctctt cgtcatatat cgctattaag atttttccgg tccattctgt
     1261 atcgatattg aattctacac ttgcatccaa atcagttatt cttgtattaa tagtaatatc
     1321 acttatatag tctttcggag ttgtatataa cttaacaggt ctatgaatac ctgcataatt
     1381 gaagaaatca aaattaacta agttttcctt ttttatatcc ccattttcct gtactgtttc
     1441 cttcataatc cccacaggta aagtactatc gtcaataata ttgtttacag caactgtaat
     1501 tcgattattc ccgtctttta caaaccgatt aatttcagac tcaaatggtg taaacccacc
     1561 tttgtgttga gtaataaact ttccattcaa aaaaactttt gcttcatgtg ttgcagatcc
     1621 aaaacgaaga actaatcttt cttctaataa tcttttagga agagtcactt ctttttcata
     1681 ccacacccat ccaacatgat ttctaatgtt ctctgtttca aataaatcat tataagaaga
     1741 gggaactgaa atcggataac tctctttttc atttattggt tctaaagcaa gttcttcctg
     1801 taagtcatca tttgtatcta atttaaatct ccagagaccg cttaagtcaa ttacttgcct
     1861 cgtctctgtc ataatgggat acaacatata aaccactcct atctaaatat taaataaatg
     1921 tatcctttaa accacaataa gcatctattg gacttactcc cttaaattca gaaagaccca
     1981 ttagtttgtc caccaatatt tctaaagttt cttgttttcc atcataagta ttaatataag
     2041 tctttacttg aggtacatca gccaaatgaa acggacactg tacagataca aaaattgttg
     2101 gaatttcatg aatgtaaaac ggtatatccg gagttccttt acttgctggc cagacaattc
     2161 gttgaactgt gcctggatta acattagcta tattaatgat caagtcatat tttttagtta
     2221 aatttttaat tggtctctta gcagcatata ttccagctat atttttaatt ttttcttctt
     2281 gcgataatcc tttcaattct tcttcaggtg aaatgtattt ttcaacttca aatccttttg
     2341 ctgacaaaat ctgtttcaat aaatcaatgg cttgaggtct atttccagca actacttcac
     2401 caaacccacc ttttacccca tgtacatcta ctagtaaaat ccttttatat ttttctggta
     2461 caataggcca aatatcttgt ttatctttaa ctaatgtaat tgcttggtca gccacctctt
     2521 taaaaattga tttattttca attaaaccaa ttttttccag agcttcttgc ttaggtaata
     2581 aaatttgttc ttttgctttt ttgtgtaatc ccaatttagc ttttaaccct aaaattcttt
     2641 ttaaggcatc taataaccga tcatcagtga taattccttt acgatagcct tccatcatat
     2701 aattaaagtc ttcatctgga tcattaaaaa atagaaatag atcacatcca gctgcaatag
     2761 aagtgggcaa taattctgat cgtttcattg ccccagtcat tcctaacata tgactagcat
     2821 cagtaactac taatccatta aatccgagtt ttcctctaag taagtcagtg attaattctt
     2881 ttgataaagt tgcaggaagt aaatcttcat ctttcagatc aggattaaaa tgttttgaat
     2941 aagctggcat atggatatga cctgccataa ttgaaggtaa tccagcttct attaagcctg
     3001 agtatacttt tccaaaagaa ttatcccatt cttccgtacc taaactatta atactaaaag
     3061 atagatgctg atctcgttcg tcaataccgt caccagggaa atgtttagct gcaggcaaaa
     3121 ttccactctc ctgaatacct ttcatataag ctaaactaag ttctaaaact ttatcagggt
     3181 tttgtccaaa agatcgacta gaaatgatag gatttctcca atttctatta atatctacaa
     3241 taggagcaaa actccagtta catccaatag ccgatgcttc aatcccagaa actctcccta
     3301 attcataagc atatttcgca tcatttgttg caccaatttt tacttctaca ccaacttcag
     3361 taccatctgt acaagctccg ttaccacctg cttccgtatt agcagcaatt aatagaggaa
     3421 ttttactttc tgtttgtaag atatagtttt gatcatatac ctcatcagca gttcctggat
     3481 tatatcgtac agctccaata tggtactgat tgaccatatt actcaagtaa tttttatcac
     3541 gactagatcc catattaaca aacaattgac ctattttttc ttccaaagtc atagaatcaa
     3601 tcgtctcttc aatccaagaa atagcatctt catctaaatt atatggtttt tttcgtaaat
     3661 ctacttttat agtcattttc tttctcctct acttattagt aagatttttc caaaaagtat
     3721 ctatatattt ttgttcaata tctacataat ctcccgataa acatttaaat aaaacatcag
     3781 ttcttaagtt ttcccatgat tctttttctt tattgaccaa aataggataa aagaaaactt
     3841 tatttttatt tgcagcttct agatcaccag gagaatcacc aatcattaat atcttatcca
     3901 aatcacaacc attttcaatt acacttcgaa taatatcttc tttttttcct ttatcttgac
     3961 aacacagttc atcaactaat gataataatt catgtctttc ccattcttct tgaacagctt
     4021 ccttattagc tgagcttaca ataattatct tcccatattt tttcagagct tttatagtat
     4081 ctctagcact taaaaagggt ttatctaatc cttttgcttg actaatctgt tgattgactt
     4141 cttccgacca ttttaaagct tttaataaat cctctgactg agaatattct atttccctct
     4201 tcaaagatgg attagacaat tcttttgtta gagataccca gtgctttaat cgattaatcg
     4261 atgttacatc atttttttct aatgttttaa ctaaacctaa aaatctgttt attccacgag
     4321 tctctgaata taaattaata ttattccaat cctgtaggaa tttttctcta tgatgaacat
     4381 caaaaatctc tgcagcaatt ggtccgaaaa agagattgtg tttataagtc atcgtatcca
     4441 taacacagcc atctgaatca atacaaaata tccaattatt catatttctc tcctatttat
     4501 gtttttgttt taaaagctcg caattttcta agactttatc tttagataat ggataaagga
     4561 aaaataaaat taatcctaca atcagaaaac aaattgatgg aattaatgtt gaagcgttat
     4621 ataacttttc taaaacatct cccgtttgta catttgctaa agagtcatag cctataatag
     4681 aaagagtcca acctccgacg cctccagcta gagcttgacc tatttttcta gcaaaagaat
     4741 ataccgcata tacggtccca tcttctcttt gatcagactt tacttcttga tcatcaataa
     4801 catcagtaat atttgcccaa ataacagtat taaacacacc caaacccata tagccaaccg
     4861 aactaagaat taagaataca tacatattac tagcttttat caaaaataag atcaaatata
     4921 ctattgctga aaagaacatt cctactgatg ccagttcttt ttttccatat tttttactta
     4981 aaaacaaagt taaaggcgaa acaactatta aaaccaaaat aggattgata aaattcatta
     5041 aaactattcc ctgtgcatca ttatagtaat caggaaatac ataattattc atactcataa
     5101 ttaataattg agcaaggagt aagaaaatag ccgcaccaat tatagaaagt aatgccctat
     5161 ttttaactaa cccttttaaa gattttaaca atgaaacttt gtattgagat tggtcaattc
     5221 ttactcgttc agtagttagt ttataacata ttaaataaca aataagtgca catatcgaaa
     5281 atattcccgc aacaattgta aaagttgatc caccacgaac tatttgagcg cctgaatcat
     5341 ctcttgtata aataaataat ggtgttaata caccaatgac caaacttgct aaagtagctc
     5401 caacatttcg gaatgtcgat agcattgttc tttcttctgt aatagaagta atagctgatg
     5461 ccattgatcc ataaggaata tttatagatg tgtaaaaaat actcccccat aatatatacg
     5521 taacatacat gtatactacc ttaacgctca tagaagctga aatcatagaa gtttgataca
     5581 ttaaaaaact tgctaaagcg actggaccac tcattcttaa aatccacggt ttaaacttac
     5641 cctgcttagt tgctttcgat ctatcaacaa tacgccccat agttacatca gtaactgcat
     5701 cgataaatct tgctactaaa aataatgttc caacggcagc tccgcttatc cccaatactt
     5761 ttgtataaaa gatcattaaa aaagaactag caaaaatgaa agtaaaatcg tttccaaaat
     5821 ctccaaacat atatcctatt ttgtctctta taccaaattt tctttccata caattctctc
     5881 ccttctgttg ttaagcgttt tcattctgat gaaagtacgt actatactct gaagcgtaaa
     5941 gagtcagtat ttatttttta catttcagag tattatttgt tttttctcct aataattctt
     6001 tatctcataa aacttatcta tatatttact agctactcga cttacttctt caaaatcacc
     6061 tttttcaaca gaagccaata aattcccacc tacaccaact gcaacaactc ctacttcaaa
     6121 ccaatcttct aagttctcta tacttactcc gccagtaggc attatattta cgtgtggtaa
     6181 tggagcttga taggctttta caatggatgg accaaaagca gaacctggga atagtttaat
     6241 gatatctacg ccagcagtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa
     6301 ataaggaatt tgatataaat tacaaatctc agctgtttgt cgatcaaaag atggactaac
     6361 gatatattct gccccagcca taattgcgat acgagcagta aacgcatcta aaaccgtacc
     6421 ggctccgatt attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc
     6481 ttcagcatta ggaactgtaa aagttagttc aatcccttta attccacccg caataattga
     6541 atttattgct ttaatggcct catccttatt tttcccacgt acaaccgcaa tgactccagc
     6601 cgcttctaac ttacttaaaa tttctacttt tttcatatct atctccttcc tttatggata
     6661 catactttgt ctagctgtac taacttacaa agaataaatt taagctttta tttcaagtat
     6721 tattttctac ttccgatttc aaaaaaatta aaataattat acgcattacg ataacaaata
     6781 ttctccacta tctgactaag atacatttca tcatctggaa ttcggcctgc tttaacccac
     6841 tcgcttagta attgacataa tactcgacga aaatactcat ggcgaggata gcttagaaaa
     6901 cttcgagaat cagtcaacat acctgtaaaa ttcgacaaca aactttgttg tgccatcgta
     6961 gtcaactgtt gacgcattcc ttcagctgta tcattgaacc accaagcaca tcccaattgg
     7021 attttttgaa tcataccccc ttggaagctt tgcattgctg ttgctagtgg aagccaatca
     7081 gttggattta atgaatataa aattgttttt ggtaagcacc cactcttttc agcggtatcg
     7141 aacaatgcta caacattttg tgtcaaacta gcttgatccc ccatcgaatc accaccgaca
     7201 tctactccaa tttgttcctt taatatactt gaattatttc ttaaagcatt taaatgcatt
     7261 tgcatcgtcc aaccatatct tgtatactca gacatcaaat gtaattgaat acctgtaaca
     7321 aattgtgcaa tttctgataa atggaaatcc gtttttcctg ctaaagcttt tattaacaga
     7381 ttgtcgagta ttttttcatc tgctatttca taaaaataag tattcaaacc gtgatctgct
     7441 aatcgacagc caatttgatg aaaatgatcc actcgttggc tcaatgcttg cttcaaccca
     7501 ttaaagtctt tgattggtat agccgctgtt tgactaagtt ttaaaatata gttcgaaaag
     7561 cctgatgatt tgatactcca gaccttatct ggacggaaag taggtaatac cttgaaagaa
     7621 ttatcctgtt tagaaagctc agtatgatac cacaagtcat ctgtaggatc atccgttgta
     7681 cagatggctt tcacatccat catctccaac aattctttcg ggcgatagga atccgtttga
     7741 attttttcgt tagcacgtcg ccaaatttct ggtgcattct tctcagaaat tgtcaaatca
     7801 atatcaaagt aacgttgtaa ttccaaatga gaccattcaa aaataggatt acctggcgcc
     7861 ttctccactg tttttacaaa agctaaaaat ttttcataat catcacctgg accactgatc
     7921 aactcttctt ctataccatt agctcgcatc aatcgccatt tataatgatc tccagctcct
     7981 ttatcattta accatacctg agtcaaattc ttgtattgct tattttctag caaatatgct
     8041 gggtctaaat gacaatgata gtcgataatt ggttggtgtt ttgcaaattt atgaaaaagt
     8101 cttttagcta tttcattctc taataaaaag ttcttatcta aaaacatatt taaacactcc
     8161 tttccttccg ttagttttta gaagagaaaa tttttcctct aaataaactc ccttatataa
     8221 ttatttactc gccccctttt caagcgcttc ccaaatacca tttaaataaa ctgcacctag
     8281 agctcggtca tataatccat aaccaggtct accggtttct ccccaaatca tccgaccatg
     8341 atctggacga atatagccat taaatccatt atcgtacatt gtttttaata tcttatacat
     8401 atctagagaa ccatattctg atttatgcgg cgcttcgtaa aaatcgagtc cattgtcact
     8461 taaatatttg atattacgaa catgtgcaaa aggaatccga tcccgttgac aaaattcaga
     8521 tagaatttca tagacatcat tatctggtcg ttctgcaata cttccagtac ataatgtaat
     8581 accattagaa ggagaatcca ctacattaca caaccaatct aaatcgtcac gatttttaac
     8641 aatacgaggt aatccgaaaa tggaatatgg tggatcatct gggtgaactg ccattttaat
     8701 cccaacttct tcacatgtcg ggataattgc tttgagaaaa tatgcaaagt tctctcgtaa
     8761 tttctcttca tcaacttctt tataggcttc taataattgc ttaatttgtg ataaccgatg
     8821 aggttcccaa cccggcaatt caaaattatt agtagaattt tctacttctc taataatttc
     8881 ctccggatca gtagggatat tttttgaaat ataggctaac gtagaagacc catcatctaa
     8941 acgataatcc aaatccgatt ttacccaatc aaatactggc ataaaattat aacagataac
     9001 ctcaatccca aattctgcca aattgcgaat cgtctgttta taattctcaa tgtattgatc
     9061 tcgtgttggt aaaccaattt tgatatcatc atgaacatta acactctcaa taactttcaa
     9121 ggtaagtcca ttggcttcga cttcatcttt taaagattta attcgttctc tcggccaaac
     9181 ctctccggct ggtatatcca ttaatgttcc aacaattcct ttcattgctg ggatctgacg
     9241 aatttcttgt agtgagattt tttcttcatt tgccccatac catctaaaag tcatttccat
     9301 agttactttc ctccaacttc tgttatatat ttattgattg ttttacgaac tgcttgagga
     9361 cctactagca tttcgcaaaa gattttttca gttttctttc ctaccccaac ttgatataaa
     9421 tctaaaccaa agatttcttt attttctaaa atttcctgta ccgcacaatg aatctttgaa
     9481 ggatcttgtt cacctaattt caatactgat aatttatcct gcaactctac caataacgga
     9541 tcaggacttg gcacaaaatt ttccccttga tcatcgactg ccagtaaata acgtaaccaa
     9601 gcagcaatag cgataggaat aaatgttaga ttttctgcat ttccgtcttt ttcgacatac
     9661 tttttaattg tttcaccata gcgaatcgct actttttgag aagtatctgt tgcaattcgt
     9721 tgtggtgtat ctggaatata gggattaggc aaacgtttat taattacttc atcaataaat
     9781 tgttttggat caataatgcc tggattttca actactggta aaccttctaa ataacctatt
     9841 cctttgatta aaccaaccaa atcatcatcc tgcatctctg ctgaaattga ttgatatcct
     9901 agcagacaac ctgttactgc caaagcagta tgaagtggat tcaagcttgt cgtaaccttc
     9961 atgatgtctg ctttatcaac cgtctctcta tctgtcataa tcacaccagc cttttccaat
    10021 ttaggccggc catttggaaa actatcttca ataactaaat aatgaactac ctcagtatta
    10081 gtgaaagcag caatattggt atgttttttt gtgtgaatga tatctaaatc ctcgagcccc
    10141 atcatcaata gatcttcctt tacattctct gaaggatttg gcgtaattcg atcaatcata
    10201 ctccaaggaa aagtcacttt ttctttatcc tttaaataag caataaattt ttcagaaaca
    10261 tatccttctt tttgccaagc ttctgcaatc gtaaaaatac tatcttgaaa acgttgacca
    10321 ttctgtgaga aattatcagt agaaaccatc gcaatcggca actctccagc attaaatctg
    10381 ctatataaca aggatgttac aatactcatc gtatgttgag gattgctagg tccagatttg
    10441 atatcagctt tgactaccga aaagaattgc cctttagaat cttttaaact gtaacctttt
    10501 tcagtaatag taaacgttgt aaactgtaat ttaggatctt caaaatattt aaccactctt
    10561 tgatagtccg ctatattctt cggattgcaa tataaactat cagccgttac tgccaaaagt
    10621 ctttttttga gttcaccatc tccactcata acaatttcca aaatgtcatt gttatagtca
    10681 tgataagctt tttccactac ttcttcatca aatgtttcac acacaactac tcctgtttct
    10741 aattcatttt gatctgctaa gcattgagca atttctgcat gaaatcctcg ataaagattt
    10801 cctccaccaa aatgaagcca tactggatgt tgctggcctt cttgcttaac ttctttgaca
    10861 tcaaaacacg gcagtagaat ctgcatttca ttgaattctt cgatagaatt ttgccaattt
    10921 ttaatcgaaa ccataattaa cctcctcaaa ctgatatgtt agttttgata cattttatat
    10981 tttaaaaccc attgtaaccg ttgtcaatat aaaaagggaa taaacttgct ttttt
//
LOCUS       DACCVU010000100        10890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCVU010000100 DACCVU010000000
VERSION     DACCVU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.100"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(164..388)
                     /locus_tag="IVW29_002233"
     CDS             complement(164..388)
                     /locus_tag="IVW29_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7839700.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IVW29_002234"
     CDS             complement(586..2166)
                     /locus_tag="IVW29_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7839701.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IVW29_002235"
     CDS             complement(2567..3943)
                     /locus_tag="IVW29_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7839702.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IVW29_002236"
     CDS             complement(4071..5189)
                     /locus_tag="IVW29_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7839703.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IVW29_002237"
     CDS             5331..5765
                     /locus_tag="IVW29_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839704.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IVW29_002238"
     CDS             complement(5843..6376)
                     /locus_tag="IVW29_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7839705.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVW29_002239"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVW29_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7839706.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVW29_002240"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVW29_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7839707.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IVW29_002241"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IVW29_002241"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7839708.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IVW29_002242"
     CDS             complement(10101..10679)
                     /locus_tag="IVW29_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7839709.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3211 a         2213 c         1862 g         3604 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt
//
LOCUS       DACCVU010000101        10876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCVU010000101 DACCVU010000000
VERSION     DACCVU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.101"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            10..879
                     /locus_tag="IVW29_002243"
     CDS             10..879
                     /locus_tag="IVW29_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7839710.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVF
                     LSYVSIDDLSNLI"
     gene            1144..1623
                     /locus_tag="IVW29_002244"
     CDS             1144..1623
                     /locus_tag="IVW29_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839711.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1656..3884
                     /locus_tag="IVW29_002245"
     CDS             1656..3884
                     /locus_tag="IVW29_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7839712.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4013..5656
                     /locus_tag="IVW29_002246"
     CDS             4013..5656
                     /locus_tag="IVW29_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7839713.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5729..6274)
                     /locus_tag="IVW29_002247"
     CDS             complement(5729..6274)
                     /locus_tag="IVW29_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7839714.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6297..6782)
                     /locus_tag="IVW29_002248"
     CDS             complement(6297..6782)
                     /locus_tag="IVW29_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7839715.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6772..7584)
                     /locus_tag="IVW29_002249"
     CDS             complement(6772..7584)
                     /locus_tag="IVW29_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7839716.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7723..9255)
                     /locus_tag="IVW29_002250"
     CDS             complement(7723..9255)
                     /locus_tag="IVW29_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7839717.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9364..10395)
                     /locus_tag="IVW29_002251"
     CDS             complement(9364..10395)
                     /locus_tag="IVW29_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7839718.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3627 a         1995 c         2090 g         3164 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
      841 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaat acaaaaaaga
      901 tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa catcatcttg
      961 gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc
     1021 aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt
     1081 agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa
     1141 ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat
     1201 aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact gggccttgct
     1261 cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa
     1321 aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta
     1381 gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca
     1441 gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat
     1501 accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac
     1561 ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat
     1621 taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa
     1681 accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga
     1741 caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa
     1801 tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca
     1861 aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg
     1921 acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc
     1981 aagaattact agaagctatt taccaaacag atacacttga agctgtactg acactgatcc
     2041 agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa
     2101 ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc
     2161 gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc cgagatgcct
     2221 ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag
     2281 gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag
     2341 gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc
     2401 ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag
     2461 aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg
     2521 ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag
     2581 gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc actgattcag
     2641 atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg
     2701 acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa gaaagaacca
     2761 ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct
     2821 tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag
     2881 aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg
     2941 aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg
     3001 acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca
     3061 tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa
     3121 aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc
     3181 ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg
     3241 aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa
     3301 agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt
     3361 caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat
     3421 tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa
     3481 gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg
     3541 aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag
     3601 cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg
     3661 gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg
     3721 ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca
     3781 aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg
     3841 aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac
     3901 gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt
     3961 ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca
     4021 agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga
     4081 tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa
     4141 agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa
     4201 tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt
     4261 tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat
     4321 caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc
     4381 gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac
     4441 atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca
     4501 tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact
     4561 attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat
     4621 ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt
     4681 tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta
     4741 cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt
     4801 agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca
     4861 agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg cttggcaaac
     4921 ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc
     4981 cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc
     5041 tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat
     5101 gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta
     5161 taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac
     5221 aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc
     5281 tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca
     5341 agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac
     5401 caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta
     5461 tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc
     5521 acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa
     5581 ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata
     5641 taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt
     5701 tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt
     5761 taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga
     5821 caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata tcgataccgg
     5881 cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag
     5941 aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt
     6001 ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta
     6061 gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt
     6121 ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa
     6181 caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca
     6241 attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa
     6301 tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt
     6361 ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat
     6421 aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct
     6481 aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg
     6541 tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga
     6601 ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag
     6661 atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt
     6721 aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc
     6781 atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat
     6841 cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt
     6901 aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc
     6961 gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata
     7021 gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt
     7081 tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag
     7141 agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg
     7201 aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt
     7261 gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg
     7321 aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt
     7381 cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat
     7441 aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc
     7501 aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc
     7561 gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt
     7621 ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa
     7681 aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt
     7741 taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc
     7801 aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg
     7861 attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt
     7921 atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat
     7981 agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg
     8041 gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc
     8101 ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat
     8161 atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt
     8221 catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt
     8281 atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc
     8341 atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa
     8401 agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg
     8461 ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat
     8521 atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat
     8581 caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa
     8641 tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga
     8701 atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata
     8761 agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa
     8821 ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt
     8881 tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt
     8941 tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac
     9001 agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt
     9061 cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta
     9121 agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt
     9181 ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt
     9241 tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata
     9301 atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt
     9361 gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat
     9421 cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt
     9481 taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag
     9541 ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg
     9601 agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc
     9661 tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt
     9721 cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg
     9781 tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc
     9841 aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc
     9901 aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc
     9961 tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc
    10021 tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta
    10081 atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt
    10141 atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag
    10201 ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc
    10261 ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg
    10321 ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct
    10381 agttttgctg tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat
    10441 ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta
    10501 ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt
    10561 cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg
    10621 aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga
    10681 aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata
    10741 ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga
    10801 agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt
    10861 ttgcttattg atggat
//
LOCUS       DACCVU010000102        10780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCVU010000102 DACCVU010000000
VERSION     DACCVU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.102"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            71..604
                     /locus_tag="IVW29_002252"
     CDS             71..604
                     /locus_tag="IVW29_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7839719.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1094..1618)
                     /locus_tag="IVW29_002253"
     CDS             complement(1094..1618)
                     /locus_tag="IVW29_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839720.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1611..2084)
                     /gene="tsaE"
                     /locus_tag="IVW29_002254"
     CDS             complement(1611..2084)
                     /gene="tsaE"
                     /locus_tag="IVW29_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7839721.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2207..3187)
                     /gene="pta"
                     /locus_tag="IVW29_002255"
     CDS             complement(2207..3187)
                     /gene="pta"
                     /locus_tag="IVW29_002255"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7839722.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3340..4020)
                     /locus_tag="IVW29_002256"
     CDS             complement(3340..4020)
                     /locus_tag="IVW29_002256"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7839723.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4146..5015
                     /locus_tag="IVW29_002257"
     CDS             4146..5015
                     /locus_tag="IVW29_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7839724.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5041..5556
                     /locus_tag="IVW29_002258"
     CDS             5041..5556
                     /locus_tag="IVW29_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839725.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5603..6166)
                     /locus_tag="IVW29_002259"
     CDS             complement(5603..6166)
                     /locus_tag="IVW29_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839726.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6221..6400)
                     /locus_tag="IVW29_002260"
     CDS             complement(6221..6400)
                     /locus_tag="IVW29_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839727.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6477..6986)
                     /locus_tag="IVW29_002261"
     CDS             complement(6477..6986)
                     /locus_tag="IVW29_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839728.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7082..7462)
                     /locus_tag="IVW29_002262"
     CDS             complement(7082..7462)
                     /locus_tag="IVW29_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7839729.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            7792..8541
                     /locus_tag="IVW29_002263"
     CDS             7792..8541
                     /locus_tag="IVW29_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7839730.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(8725..10002)
                     /locus_tag="IVW29_002264"
     CDS             complement(8725..10002)
                     /locus_tag="IVW29_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839731.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10081..10725)
                     /gene="pcp"
                     /locus_tag="IVW29_002265"
     CDS             complement(10081..10725)
                     /gene="pcp"
                     /locus_tag="IVW29_002265"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7839732.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
BASE COUNT         3027 a         2210 c         1946 g         3597 t
ORIGIN      
        1 gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg
       61 ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag
      121 tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac
      181 attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca
      241 agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt
      301 tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg
      361 atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag
      421 tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt
      481 gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat
      541 cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa
      601 gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc actccgagtc
      661 acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg
      721 aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa
      781 gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta
      841 ccgcagatgc aagcttttgg acaccgttta ccaagaggtt gtgaaatcag cgttttgacc
      901 tgagtattag agaaacaaac ggcaaaatag ttcctcatat tttgccgttt tttgagctaa
      961 taccgaaggt cagctgattg aacaccgttt attcaaaaaa aatggactgc gacacgactt
     1021 ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt ttctcactct
     1081 tttcctattt cttctattca atcaaataac tcattttata tacatctaaa aattcaccta
     1141 gatctgttct tgcccctcta ggcatgaccg cttcgatttg gaagcccatt tttcggtaaa
     1201 ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa agtacaccca
     1261 tttccttcgc ccaactgatg atttcttcta gcataagagt gcctagtccc attccccaat
     1321 attcctgcag aatactgata ccgacttctc ccacgtgaga caaacgaaat tgactgtctg
     1381 ccttgacaga tgctgttcca ataatcgtcc cttcatagat tgcaagcagc aataaattat
     1441 tattgctttc gtatatataa tccaactctt cttctaaggt ttctggtggt aacaggagtt
     1501 ctgcttcatc caaaactaaa aattctgtct ccttattaac ttgtcccatc atcaaaagaa
     1561 tgtcttttgc atctcctggc actgcttccc gcaagatgat cccaacttct tcactcatga
     1621 ttttcagccc atttttctag aattcgctgt ttgaaggttt cactttgctc cccgtatgag
     1681 attagtttca cataccgctt ttctaaatct gtatcacttt tttctaaaat cagttccaaa
     1741 taatcctccg gcagtgcttc ttctaataga tttccccatt caacaacgga aagcccatca
     1801 ccttcaaaat attcatctaa ccctaaatct gctccgctag ctgctacccg ataaatatcc
     1861 atatgataca acggaatccg accttgacta tattctctaa taatcgtgta tgtcggactt
     1921 ttgatcattt gcgttatccc taatccttgg gcaattcctt tcgtcaacgt tgttttaccc
     1981 gcacctagat caccagtcaa tatcagattg tcacctggca ttgcggaatg accaattgcc
     2041 tctgcgaatt gttctgtcat tgttaatcct gatagttcga tcatttttca tcttctcccg
     2101 ctcttctttc tttttgccag tatatcgcgt aatgaagttt ttttaaagaa aaaatgaact
     2161 agtcaagaac agcagctgct gtttttggct agttcgcgtg tatttgttaa tcaatcaatg
     2221 tttgtgctgc tgtgataatc gataatttat atacatcttc ttcatttgct ccacgagaca
     2281 aatcagaaac cggtttgttc aagccttgta gaattggtcc gattgcttca aagttaccga
     2341 aacgctgagc aattttatag ccgatatttc ctgactgcaa ttctggaaat acaaagactg
     2401 ttgcttgacc agctacttct gaatctggcg ctttcagttg tgcaactgaa ggaacataag
     2461 aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt ttagcgattt
     2521 tagttgcttc aactactttg tctacttctg gcgatttcgc tgagcctttt gtcgagaagc
     2581 tcatcattgc tacttttggt tcgatatcga acaattctgc tgtttttgca gaatctacgg
     2641 cgatttcagc caattcttgc gcagttggat tcacgttgat cgcacaatcc gaaaagacat
     2701 atttttcttg atctcttcca cgtaccatga tcatcgctcc gcttgtccga ctgatgccag
     2761 gctttgtttt gatgatctga agtgctgggc gaacagtgtc gccagtcgaa tggatcgctc
     2821 cagagaccat tccgtctgca atccccatat acgtaagcat cgtaccaaag taattcacat
     2881 cttttaaaat cttttctgct tgttctttgg atgcctttcc atttctacgt tcaacaaaag
     2941 tatccaccat ttcatcccac ttatcgtaat tatcagggct gatgatcgta aagctagatg
     3001 ttttgattcc tctggcatgc gctgcatttc ttatttcttc ttcgttccct actaaaatgg
     3061 gttccacaag ttcttctgct ttcaaccggg cagctgcgcc taaaatacga gcatcagtag
     3121 cttccgggaa aacgattttg atcccacggc gaacaatttt aaatcttaaa ctatcaaaca
     3181 attccacaag atgacctcca gacgttttta tatgtattat catacactat tccagaaaaa
     3241 aagaacagtt tattttatct gtatcagtca aaaatgctgg aaaaattaag tttttcacat
     3301 acaaagcgaa ctgcactata acaggaaagc gctgttttat tacactgttt caggcagttg
     3361 ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg agaagggttt
     3421 ggaaccaaaa aagccacgac ttgcagataa cggactggga tgtggagatg ttatgatgat
     3481 atggcgactc ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc acaagataaa
     3541 cacgattggt ttttcccgtt catttaattt ttcaatgatc gtgtctgtta atctttccca
     3601 acctttcccg cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg tgttgagtaa
     3661 aaggactccc tgtttagccc atgacactag atttccatgt tgaacaggtg cgattccaag
     3721 atcatcataa agctctttat agatatttct taacgatggc gggatcttta cgccaggttg
     3781 cacagaaaaa gataagccat gagcttgatt ttctccatga taaggatctt gtcctaaaat
     3841 caccacttta acttcttcat aaggagtcaa ttccaatgct tcatagatat gatacatatc
     3901 tggatggatc ttttgggacg catattcttt ttttaaaaat tctcttagat gcagataata
     3961 atcttttgaa aattctgagg acaaaacttc ttgccaactg ttatgaatga tttctttcaa
     4021 aaactccacc tcctaataaa aagaataact cacattttct tcaatcacaa gggacaatct
     4081 tcaccctcag cacttgatta atatggtaaa ctaggaacaa gaagcagaaa ggaacgataa
     4141 tcattatgat caaattaatt gcatcagata tggacggcac attattagat gcccatatga
     4201 gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt gttgagttca
     4261 tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag gtaggaatcg
     4321 attgtgcgat gatcactttg aacggggcac aggtatttga taaagaagga aattcattgt
     4381 ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt agatattcta gatgctaacg
     4441 gtatttacta tgaagtagca accaacgatg gtctttattc tgaaaatcaa gcgaaacgaa
     4501 ttgaaagttt tgcttccatg gtggctaccc atttacctca ccttacgtat aaaatggcga
     4561 ttgccatggc ctcagctaag ctggagttat tgcacatcac atacgtggac agtattcgtg
     4621 ctattttgga agaaaagaat ctggaagttt taaaaattat ttgttttcat acagaagggc
     4681 cagctgtatt aggtccagtt ggaaagcaga tttctctttt aggcgatcta gccgttactt
     4741 cctctggtca aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga attgctgttg
     4801 cccatgtcgc tcacgagcgt gggatttctt tagaagaagt catgacgatt ggcgataact
     4861 tcaatgatgt cagcatgctg caagtagcag gagtcagttt cgcaatggga aatgcagaaa
     4921 tcgaagtaaa agattatgcc aaatatacaa cggacacaaa ccttgaatct ggcgtaggaa
     4981 aagccatatt gcgcgcgatt gcagaagact tgtagtttta tctagaaaag agggatatag
     5041 atgtcagaat ttttcattca ggaaaagcag cttggacgtg caactcggac aattgtcaaa
     5101 aacgaaaaag gacattccct gtttttgatg gttggtcgct ggggaacaaa gggcgatgca
     5161 ttgtctcttt atgccatgaa tggcgatctc gttgctcaca tcaagcagat aagcctgaca
     5221 ttcggtacgc gttttgagct gtataaagag ttcgaaaagg tgggaaccat gcagaaaata
     5281 ttcaattggc ccggtgactt ttattatatt cgtagacttc actggacagt ccaaggtgat
     5341 atctataatc atcaatatca aatccatcat ttcaatcagc aaatcatgaa aatggataaa
     5401 gcaacacttt tttcaggaga ttactatgta ttggatgtct caaatgatga agatgctcca
     5461 gtttgtattt gtattgctgc ggttttagat tactggctct accgtaaaga caaaagtcgc
     5521 gataagcctg tttcttaccg acttaactta agttaaaaag atgtacctga aatgcggaaa
     5581 tacgcatttc aggtacatct ttttagatat tcttttgcat atgatcataa agatgaccac
     5641 tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt ttagcctttg
     5701 gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc gccaatttag
     5761 gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct gatacactga
     5821 tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa cgtgtagacg
     5881 catctagctg atatttctct aagataggta ccagtggctg atcgatgatt gattcttctt
     5941 catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct acgatacctc
     6001 gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc tctaaagtct
     6061 tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc actaagatca
     6121 atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt tcctctcctt
     6181 ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa aaatcttcaa
     6241 tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt cggcaataag
     6301 ggcagacgac tggcatttct ttatcattat gttcgactac attattgact tcttcgtatg
     6361 caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa cttcctttca
     6421 tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc cttcttctat
     6481 gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt ataataagga
     6541 cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc gtagtagctg
     6601 actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc tatatcaaaa
     6661 agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt ggatggattg
     6721 actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt cgtaggattc
     6781 tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt tgtttcgttt
     6841 tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact aatatcatcc
     6901 atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac gatgagggca
     6961 gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt ttaaagaata
     7021 tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa ttttgttatc
     7081 tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc attttttttc
     7141 ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc aattctgcaa
     7201 ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa gcttctggat
     7261 agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca cttcctagtt
     7321 tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt acttcaaatc
     7381 ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg atggctatat
     7441 gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct cggatctata
     7501 ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt tttccttttt
     7561 ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt cctgttccca
     7621 atataataaa tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa attatcataa
     7681 cacaaattat ttataattga aagactagtt ttttttcaag aaaagcatac aatagattgt
     7741 gttagtaaat acagaacaca ctacatttag tgtcttaagg agtcctcata catgaagtca
     7801 tattttcagt ttttagctca tcagctaaaa ggctggccgc aacaaaatta ttatcttttc
     7861 tttttcagtc ttggctgcca ggtcatgact cttgtcaata gtcccatcac ttggctgtcc
     7921 gttattacat ttatcggtac cacgcttggt gtattgtgta ttttatccat caatgcggca
     7981 aaatcagtca acggtatttt aggtattttg tctgcgattt gttttattat cgttgggttt
     8041 tcagcaaaga actatttaag tatcggtgaa cagattgctt atatgatcac attggatatc
     8101 cccgtattgt taagtaaaga atggaatcat aatatggctt caaaaatccg aaaattcact
     8161 ggaaaaagct ggtttatcgc aatcatcagt actttccttg tctatcttgt ttcaggattc
     8221 ctgattggtc gttatacaga tgatcctcgg ccgtgggtag atgccattgc tttttctatt
     8281 agcttgactg ctggtatcat cagtttcttg cgttacaaca accaatatta ctggtggtta
     8341 gcttcagggc ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg atctgcaagt
     8401 ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc atttaccgtt
     8461 tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga agaatactac
     8521 aacgcacaac aacacgcata aaaaaactga gcgtgggaca aaagtaaaga acacttttgt
     8581 ctcacgctct aaacacgtat aaacggcgga atcagaagca actccttcgg aaataagctg
     8641 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     8701 cgcttctgtc ccagcctcta ttttttatag taattccttc agatactttc ttgcctcatc
     8761 attgaaacgc agtgattctt ctaacttctg ataatattcc aacgcttgtt tctgagcttc
     8821 agtaaaacga taatgttttc ctttccattt gtcgattgtc tcttgcacat ttccagattg
     8881 gatatctttc actactagtt gccatttttc ttttgagact gtacggtctt tcttatcagc
     8941 agattgtata gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc tgactatccg
     9001 aggaatgctg tcgaagaacc cttctgtaaa accatagcga caatattgct tccaaataaa
     9061 tagatagatt tttggttgga tgaatcgttg gtttttcgca aaatattcca tctgagcagt
     9121 gacttctggt gttcgttgac gttttgtctt tctcaacacg gaaataaatg tttcaaaata
     9181 agcattagga tgtactggca cttcttcata tacgatggat tgccaatctt tacgtaaaaa
     9241 tgcgcgatag ttgatcaaga attcttctac atcctttact aattcttctg caaagtcgtc
     9301 aaatagttct ttttttatct gtcgcttttt tgactgccca ttgatgtgtt cacttatttt
     9361 cttgatttca ttttccaatg cttgataatc cgctatgtaa atcggcattt ctacttggcg
     9421 aaattctgcg agccgctgag ctaattttat atccggtaaa tatggctgtt tcatccgacg
     9481 attttggtac aagacaaaac agtatttttg ccatagtcct tcaggaaaat cgatattctt
     9541 ctgcttaagc cataataatt cgtctaaggc cagatcaact tttgtcattc cctgatggat
     9601 cgaagctgcc actgtcggag agacatgcag gtgatcggca aagtggttga tgcctagttt
     9661 taggatcttt ccggtttttg gcgatttaac gatgtattgc acttttaatt ctctaccaca
     9721 ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc
     9781 agaaaacacc caatctgttg cagctaaata atgttcttcc ataaaacgag agctgattag
     9841 atatcggcgg tgcttatcga ctagttcccg ctgttttttc gttaattttg catagatctc
     9901 ccaacgttga ctgcttgtgt attgtttaga aattttgatg ggatagcttt tgctgatttc
     9961 ttttggaaac cattcttcaa agacggattg ccattcttcc acggctgttt ctttcctttc
    10021 taattacaaa aagctggcta ctaacataac gttagcaacc agccagtgtt tcttgctaaa
    10081 ttagtgagtt gttccggcat ttccggaaat atcttcttta ttttctactg ctgcttcaac
    10141 tgcataagtc aaagaagaag caatgtcttc taaagacata gaaggcgtac ccactggacg
    10201 atcgatcact tgatcgggtg agaatggcac gtggatgaac ccacctcgaa ttgccgggaa
    10261 ttgtgtttcg atcaaatgaa gcaaacgata catgatatca ttgcagacaa atgttcctgc
    10321 tgtataagag atgtacgctg gtagaccatg atcttgtaca ttttttgtca ttgctttgat
    10381 tggcaatgag gtaaagtaag cggctggacc gttttcttct aaagccgtat cgaatggctg
    10441 atttccttca ttatcaggga ttcttgcttc tgctagattg attgcgacac gttcgaacga
    10501 gactgctgac cgcccacctg cttgtccgac acagatcacg acatctggtt ggtgctgttg
    10561 gatggcttct ttgactactt gactgctttt ttcgaatacg gtcggtactt ccactttgat
    10621 gatttcagca ccgctgattt catctggcat tttttttact gcttcatagg ctggattcac
    10681 tttgtcccca ccaaatggat caaaacctgt tactagtact ttcatctgtt gttcctccgt
    10741 ccgattgatt taacatatgg ttatctttta tcataccatt
//
LOCUS       DACCVU010000103        10702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCVU010000103 DACCVU010000000
VERSION     DACCVU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.103"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(25..144)
                     /locus_tag="IVW29_002266"
     CDS             complement(25..144)
                     /locus_tag="IVW29_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7839733.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IVW29_002267"
     CDS             complement(159..1343)
                     /locus_tag="IVW29_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7839734.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IVW29_002268"
     CDS             complement(1599..1853)
                     /locus_tag="IVW29_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839735.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IVW29_002269"
     CDS             complement(1878..3020)
                     /locus_tag="IVW29_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP7839736.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IVW29_002270"
     CDS             complement(3080..4432)
                     /locus_tag="IVW29_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7839737.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IVW29_002271"
     CDS             complement(4503..4847)
                     /locus_tag="IVW29_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7839738.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IVW29_002272"
     CDS             complement(4857..5231)
                     /locus_tag="IVW29_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7839739.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IVW29_002273"
     CDS             complement(5244..5558)
                     /locus_tag="IVW29_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7839740.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IVW29_002274"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IVW29_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7839741.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="IVW29_002275"
     CDS             complement(8978..9640)
                     /locus_tag="IVW29_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839742.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10257..10487
                     /locus_tag="IVW29_002276"
     CDS             10257..10487
                     /locus_tag="IVW29_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839743.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            10493..>10702
                     /locus_tag="IVW29_002277"
     CDS             10493..>10702
                     /locus_tag="IVW29_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839744.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
BASE COUNT         2953 a         2365 c         1814 g         3570 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccagaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcatctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gacttttcac aaatttcaga tttccaagga agttttccgt gtttatcttc
    10021 tgatttcttt gatacgagca gcttttccgt gtaatgcacg taaacaaaac cactatattc
    10081 tattatgtat ctaataccct caaaacagct ataaatcaac aataccgaca tataataaac
    10141 actatgttca atctgagata tatcaaaggt aaacgtattt ttgccccctt tttgcccctc
    10201 aaatatcgct ttacaacgag aacgtaagtt cgtataatcg ttttgaggtg atttttatga
    10261 tggaagactt tattagaaaa aatatcagtg atgaatatgc agacttttat gagcaaagca
    10321 gcaaaaaaga taaattccag atggatgttt caatcttagc tatattagct ttttccgaaa
    10381 ataaccaacc tgtaattgcg aaaaaagaaa cagtattatc tgaaggcaaa ataaaaactc
    10441 gatatatatt agaggtagaa actaagttta aaaatagatc ggagtaatgg ctatgctttt
    10501 taatgagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    10561 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    10621 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    10681 tgtatacttt ttgtttgata tc
//
LOCUS       DACCVU010000104        10701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCVU010000104 DACCVU010000000
VERSION     DACCVU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.104"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(244..1092)
                     /locus_tag="IVW29_002278"
     CDS             complement(244..1092)
                     /locus_tag="IVW29_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7839745.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1256..2158
                     /gene="gnd"
                     /locus_tag="IVW29_002279"
     CDS             1256..2158
                     /gene="gnd"
                     /locus_tag="IVW29_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7839746.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2160..3161
                     /locus_tag="IVW29_002280"
     CDS             2160..3161
                     /locus_tag="IVW29_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7839747.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3158..3442
                     /locus_tag="IVW29_002281"
     CDS             3158..3442
                     /locus_tag="IVW29_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7839748.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3475..4272
                     /locus_tag="IVW29_002282"
     CDS             3475..4272
                     /locus_tag="IVW29_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7839749.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4286..5119
                     /locus_tag="IVW29_002283"
     CDS             4286..5119
                     /locus_tag="IVW29_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839750.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5137..5994
                     /locus_tag="IVW29_002284"
     CDS             5137..5994
                     /locus_tag="IVW29_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7839751.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6016..6525
                     /locus_tag="IVW29_002285"
     CDS             6016..6525
                     /locus_tag="IVW29_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839752.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6562..6963
                     /locus_tag="IVW29_002286"
     CDS             6562..6963
                     /locus_tag="IVW29_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839753.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7075..7428
                     /locus_tag="IVW29_002287"
     CDS             7075..7428
                     /locus_tag="IVW29_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839754.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7462..7905
                     /locus_tag="IVW29_002288"
     CDS             7462..7905
                     /locus_tag="IVW29_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7839755.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7922..9007
                     /gene="uxuA"
                     /locus_tag="IVW29_002289"
     CDS             7922..9007
                     /gene="uxuA"
                     /locus_tag="IVW29_002289"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7839756.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9000..9713
                     /locus_tag="IVW29_002290"
     CDS             9000..9713
                     /locus_tag="IVW29_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7839757.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10465..10665
                     /locus_tag="IVW29_002291"
     CDS             10465..10665
                     /locus_tag="IVW29_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7839758.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3583 a         1692 c         2314 g         3112 t
ORIGIN      
        1 aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta
       61 tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca
      121 tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca
      181 ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg
      241 gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc
      301 tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat
      361 acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac
      421 atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc
      481 agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat
      541 caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc
      601 cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac
      661 aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc
      721 cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact
      781 agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg
      841 gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa
      901 tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc
      961 acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc
     1021 ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat
     1081 ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat
     1141 taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc
     1201 tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga
     1261 aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca
     1321 ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa
     1381 tgacattcct gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat
     1441 catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc
     1501 aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat
     1561 ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg
     1621 aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt
     1681 tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg
     1741 tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat
     1801 gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga
     1861 acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat
     1921 ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc
     1981 aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat
     2041 tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt
     2101 agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa
     2161 tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc
     2221 acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc
     2281 caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc
     2341 cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg
     2401 gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta
     2461 tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt
     2521 ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc
     2581 gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa
     2641 ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt
     2701 atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc
     2761 aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact
     2821 atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata
     2881 gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga
     2941 tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt
     3001 tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag
     3061 aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg
     3121 aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg
     3181 tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt
     3241 ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc
     3301 atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta
     3361 tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa
     3421 aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt
     3481 tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt
     3541 gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca
     3601 gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc
     3661 cctgttccat atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag
     3721 ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact
     3781 gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca
     3841 caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt
     3901 ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac
     3961 gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat
     4021 gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct
     4081 ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat
     4141 aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt
     4201 cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc
     4261 agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat
     4321 tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt
     4381 acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca
     4441 agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg
     4501 atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg
     4561 ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta
     4621 cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt
     4681 agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc
     4741 atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc
     4801 tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg
     4861 agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct
     4921 ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat
     4981 ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg
     5041 gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga
     5101 ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa
     5161 acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat
     5221 tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga
     5281 atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa
     5341 cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta
     5401 atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg
     5461 caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga
     5521 gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca
     5581 caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt
     5641 ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta
     5701 aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg
     5761 atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc
     5821 ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta
     5881 gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca
     5941 ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa
     6001 gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat
     6061 gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac
     6121 gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca
     6181 ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa
     6241 tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta
     6301 aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat
     6361 acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa
     6421 gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct
     6481 aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa
     6541 aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt
     6601 tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca
     6661 ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta
     6721 gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt
     6781 acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac
     6841 caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt
     6901 tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta
     6961 taaacaggta tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct
     7021 cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta
     7081 gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt
     7141 tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt
     7201 tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat
     7261 aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt
     7321 agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa
     7381 acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca
     7441 aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc
     7501 gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg
     7561 tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact
     7621 atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac
     7681 aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc
     7741 atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca
     7801 ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg
     7861 catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa
     7921 gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt
     7981 tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga
     8041 tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga
     8101 actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg
     8161 agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg
     8221 attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga
     8281 aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc
     8341 aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga
     8401 agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga
     8461 agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc
     8521 agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag
     8581 agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa
     8641 tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat
     8701 catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg
     8761 agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca
     8821 tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag
     8881 aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact
     8941 cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta
     9001 tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa actggacgtt
     9061 tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag
     9121 tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt
     9181 acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa
     9241 tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg
     9301 caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa
     9361 tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg
     9421 taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc
     9481 ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag
     9541 cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga
     9601 agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt
     9661 atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag
     9721 gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag
     9781 aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac
     9841 aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc
     9901 atatgagggt ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg
     9961 taacacctgt cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat
    10021 ctgtatccaa ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc
    10081 tttctttaaa tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag
    10141 tataccacca attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata
    10201 aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg
    10261 attcgaagac aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta
    10321 gaaaaagcat gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc
    10381 tacatagata aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg
    10441 atacgtatca ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac
    10501 aaaggttttg gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct
    10561 atccaaggtg acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa
    10621 gatggtcaac gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt
    10681 aaccgtcttt tcgaaagaaa a
//
LOCUS       DACCVU010000105        10651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCVU010000105 DACCVU010000000
VERSION     DACCVU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.105"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            23..646
                     /locus_tag="IVW29_002292"
     CDS             23..646
                     /locus_tag="IVW29_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839759.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVW29_002293"
     CDS             1096..2145
                     /locus_tag="IVW29_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7839760.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVW29_002294"
     CDS             2142..3023
                     /locus_tag="IVW29_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7839761.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IVW29_002295"
     CDS             3026..3796
                     /locus_tag="IVW29_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7839762.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IVW29_002296"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IVW29_002296"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7839763.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IVW29_002297"
     CDS             5107..6270
                     /locus_tag="IVW29_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7839764.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IVW29_002298"
     CDS             6387..6797
                     /locus_tag="IVW29_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7839765.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IVW29_002299"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IVW29_002299"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7839766.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IVW29_002300"
     CDS             9337..9654
                     /locus_tag="IVW29_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839767.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IVW29_002301"
     CDS             9814..10050
                     /locus_tag="IVW29_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7839768.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IVW29_002302"
     CDS             10047..10445
                     /locus_tag="IVW29_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7839769.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3717 a         1773 c         2253 g         2908 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat t
//
LOCUS       DACCVU010000106        10619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCVU010000106 DACCVU010000000
VERSION     DACCVU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.106"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            291..2237
                     /locus_tag="IVW29_002303"
     CDS             291..2237
                     /locus_tag="IVW29_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7839770.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2240..2695
                     /locus_tag="IVW29_002304"
     CDS             2240..2695
                     /locus_tag="IVW29_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839771.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2709..4037
                     /locus_tag="IVW29_002305"
     CDS             2709..4037
                     /locus_tag="IVW29_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7839772.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4071..4355
                     /locus_tag="IVW29_002306"
     CDS             4071..4355
                     /locus_tag="IVW29_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839773.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4357..4872
                     /locus_tag="IVW29_002307"
     CDS             4357..4872
                     /locus_tag="IVW29_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP7839774.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4888..5550
                     /gene="rpe"
                     /locus_tag="IVW29_002308"
     CDS             4888..5550
                     /gene="rpe"
                     /locus_tag="IVW29_002308"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7839775.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5557..6129
                     /locus_tag="IVW29_002309"
     CDS             5557..6129
                     /locus_tag="IVW29_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7839776.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6316..7836)
                     /locus_tag="IVW29_002310"
     CDS             complement(6316..7836)
                     /locus_tag="IVW29_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7839777.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8079..8264)
                     /locus_tag="IVW29_002311"
     CDS             complement(8079..8264)
                     /locus_tag="IVW29_002311"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP7839778.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8248..8430)
                     /locus_tag="IVW29_002312"
     CDS             complement(8248..8430)
                     /locus_tag="IVW29_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839779.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8671..8889
                     /locus_tag="IVW29_002313"
                     /pseudo
     CDS             8671..8889
                     /locus_tag="IVW29_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9384..9983
                     /locus_tag="IVW29_002314"
     CDS             9384..9983
                     /locus_tag="IVW29_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7839780.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10047..>10619
                     /locus_tag="IVW29_002315"
     CDS             10047..>10619
                     /locus_tag="IVW29_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839781.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3511 a         1670 c         1982 g         3456 t
ORIGIN      
        1 tattataata aaaaaatacg aattttagtg acgccattat attgttttta tttttcaatt
       61 gtaatatttc atattacttc cataataagg agtattttct attggaatat cttagctctt
      121 ttaaggattt ctcctttttt gtttctcatt ttttaagatt atttggattt cctttacaac
      181 aaacttccac aagtattatt gacatagatg taattgaggg aagggggttc cagtgctaga
      241 ataaataatg aaaagaggta acaaatgcta gcgttaagag gtgaacaagt ttgattgaaa
      301 gacatgtaaa tttattaaaa ttattaagtc gacaagaaga ctttaaaccc gcttcttttt
      361 ttagtgataa attggcagtg tcgaccaaga ccatttatca agatattgaa ttgttggaag
      421 attatgtagt taattaccaa gtaacaattg agcgattacc ttctttgggt atcaaactca
      481 taggtgatag gaaaaacgtt gaacatttga tttcatcggt agaaaattat cttgaaaaag
      541 atatttattc tccagaaaaa agacgtgtcg agatcattaa agaagttttg tttggtgata
      601 agcctgtttc tttaactcat ttgtctcaaa gttttatggt tagtaaaaca tcgttataca
      661 atgacctgag aattatcaat aaaattattg gcagtggaga agttaagttg cagtcatctg
      721 cagaaggaat tagttttact ggtaatgaac aagcaattca acgagcaatc aagcaacttg
      781 tattctatta tgcgaatgac atggaatcaa aaacattttt tcaagtttta aatatgttgt
      841 ttgattcaaa agttattagt gtcattcatg atttgctatt caatgattac agtgaactga
      901 ctgaaaaggt ttctgattac tatgttcggt cattgatttc tactttagtt atccaatgta
      961 accgtttact gaaaggtcat catttaactg ttgaagaaga attcctcttc aatagcattc
     1021 gctatatgga aacgtacatt gtggcaaatg gtttaattga agaattgaag gcaaaattga
     1081 atcttccatt ttatgacgca gataaagaat atctatgtcg tcaactattt gcacatcgtg
     1141 tgacaaataa tttaaaaacc agcaatgaag agtacgctat gagcgttcat aaattgattg
     1201 aaagaatgag tgagattgaa aaaattgatt tgactcaaaa tgatcgatta tatcaatcct
     1261 tattgtatca tgtgccagca atggtccttc gtttaaaaaa aggtattaaa attcaaaatc
     1321 cgttattaga aaatatcaaa tctcaatatt cagaactctt tagcatagta tggtacgcca
     1381 tgtccatcct tgaagaagaa tttgatgtga ctttaacgga cgatgaagtt tccttaattt
     1441 tgattcattt tcagattgct attgaagatc agtccaaagc aaataatatc atcattattt
     1501 gccaatacgg tatttcttct gcgcaattaa tttacaataa agttcgacgg tttttaccag
     1561 caaaagataa tgtagaaata tcaactttag ataagtttca tttagctgaa attggggaag
     1621 ttgatttgat tatttcctct ctttcctttg atgcaggtgg tataccctta gtaagggtat
     1681 ctccattggt aagtaaaaca gattatatga atattatgga agcttatact aaatatttaa
     1741 tagaagaacc ttcgcgattt attcaaaatg aactgattcc taaagcggtt aaagttccca
     1801 ccatatctaa atttgttcaa tcatctttag taaaaacagg agtacaggtg gaaagcaaag
     1861 aagaatgttt ggatttgatg atttccgaac tagaagccaa ttcattagtt actgaagagt
     1921 ttcgtgcttc tatcttctct agagagaaaa tggggaatac taatttagaa agtggtgttg
     1981 ctttaccgca tgcgaatcca tcaatggtca ttgaatccaa catttttatt gtagcattaa
     2041 aaaatgcgat taattggggc gatcgaaaag tcaaattagt cattatggtt agtttagctg
     2101 aagaagatgt atctgagatt agaggagtat atgaagaact ttaccaattt attgggcaaa
     2161 aagaaatggt cgatcgcttg ttacaagttc atgatagcga aaaattacta agaatttttc
     2221 aacaaaaagg agaataaaaa tgtatctaga taaagaatta tgtttatttc aattagaagt
     2281 gaaagatcaa aaagaattgt ttcaagtaat gtctgaacaa ttaaaaaacg ctggttgcgt
     2341 gaaagatagt ttcttagaag gtatcaccaa ccgtgaacaa gaatttccaa ctggattaga
     2401 agtaaatcaa attgggtttg ctatcccaca tacagattcc gcacatgtaa atagctctca
     2461 aatctgtttt gcctcattaa aggaaccact ggtattttca gatatgacag ataaatctca
     2521 tgaaatacct gtacgtttag tctttatgtt ggcaatgtca caacctcatg aacaaattga
     2581 tacactacaa aacttagtta gcctatttca aaatgaagaa aaagtaaatg aattacttgc
     2641 atgtaccacg aaagaagcat ttattgagtt agtacattct gcaggagttt attaggagga
     2701 atagagagat ggatattgta ttaaatgcct ttgaatggtt tattggatta gggtcaaacg
     2761 tctttttacc actgattatt tttgtaatcg ggatattatt tggattaaaa cctggtaaag
     2821 catttgtttc cggcgttact gttggtatcg gttctattgg tttaggatta gttttaagtt
     2881 tattatcaga tggtttagga acagcaattc aacaaatggg ggaacaatac ggtggtggcc
     2941 tgaatatcct agatattggc gtaggtgttg gtggaccttt ggcttactct acatcattgg
     3001 gaattttaat gattccaatt tcattaattc ttaacttcat atttgtggtt ttgggttgga
     3061 caaaaacatt gaatgttgat atttggaact tttggttcgc aattttccta ggtatgatta
     3121 ctcaatctgt tacgggaaac ttttggtttg gcttaattgg cgcgattgtc gcagtaattt
     3181 tacaatggtt cttagcagat cgtacacaaa aaactgttag cgaatttttc ggctatccag
     3241 gaatctctat cacacatatg atgggattat caggagtact atttgccaaa ccagtaaatt
     3301 gggtttttga tcgtattcct ggatttaata aaatagatgc agatgcagaa acattaacaa
     3361 aacgtttcgg tgttttaggt gacacagttg ttattggcct aattatcggt attgtagtgg
     3421 gtattatggc aggctatgac gtagcaggaa ttggaaactt aggaattatg actgcggcag
     3481 tgatgaagat tatgcctaaa atggtagcaa tgtttatgga aggtttaatg ccaattgcag
     3541 aagcggcgaa agaatttacg aataaaaagt taaatggtcg ccaagtaaac attggaatgg
     3601 atgcagcgtt aactgtgggt catccaacta ttatgtcaac ttctctattg cttgtaccaa
     3661 tctcattatt gctagcacta attcttccag gaaataaagt attacctttt ggagatttag
     3721 cattctttgc tttcgccatc tgtttaatga ttccttattt taaaggaaat attgtgagat
     3781 cattagtagg atgttcgttg tatgttgtta caacattata tatggcaaca tggttggcac
     3841 caattatcac tgatgtgttt acattagcaa gtttcgatgt ggggacagca ggattagtaa
     3901 ctatgttgtt atgtggatta tggccagtag ggttgtttgt tttattagca tcttactttg
     3961 gtgtaattgg aataggtgga ttaggagttg ttttagtagg tgcttctatc tacatgaaca
     4021 aagtgaagaa agcttaatca taaaattaaa ataagaaaga agagatcaat atgaaaaaat
     4081 tattagttat ttgtggagca ggtcatgcta cttctacaat tgcagtagca aaattatcaa
     4141 aatggttgga agaagaagga ttaacagatc aagtgaaaat ttatcaatct aaaattgctg
     4201 atgagctaaa taaaattgat gaatatgatg ctgtagtaag tacaacaatt gtccctgatg
     4261 taattaaagc taaagtaacg aatggcttgg gcttattaac aggtattggt acagatgcca
     4321 tttatgcaga tgtaaaagaa agattaggtg tttaatatgc gttttcatca tgacactttg
     4381 actattgaaa cagtttcagg gcgtccttcg tatcatgata ttcgaaaaga acttttagat
     4441 acagttgata cagcaggtat tgagaatgga atgttagtaa tttcgagccc gcatacaact
     4501 tgctcactct tttttgaaga aacgatgcat gacacaaact tttttggtga tgattattta
     4561 catgtagaca tcaacaatgt aatggaaacg attattccaa aaatgacgag tgaaaatcaa
     4621 tacaacagtc cggggccaaa acacattgat tttggaacaa gattatctga tcccaattac
     4681 cctgcagaaa aatgggtcat gctaaatacc gatgcacatt tacgttcatc tatttatgga
     4741 accaactcaa tgactttaat tgttcaagaa ggtaaacttt tattaggaga aatgggccgt
     4801 gtctattttg tcgattggga tcaactaaga gagcgaacta gaacaatcaa tctcatgatt
     4861 atgggagaat aaacggggga atagagaatg acaattgctc catcattgtt aaatgcacct
     4921 atcgcagaag ttaaagagtt atttgaaatc ttaaaagaaa agaatgttga ttatttgcat
     4981 attgatgtaa tggatggctg ttttgtacca gatatggctt ttggaccaag tgttattaaa
     5041 gatattcgtc cactaacaga cgttgtgttt gatgttcatt tgatgattga tacaccagaa
     5101 aaaattgtca caagatttgt agaagcagga gcagatattg ttaccttcca cattgaagct
     5161 accaaacacg caatgaaggt cattcagtta gtcaaggaag caggagcaaa agtagggatt
     5221 acttttaatc ctgcaacacc agtcagtttt attgaaccag tattatcttt agtcgatcaa
     5281 gtattagtaa tgacaatcaa tccaggaaca gagaataagc attttattca agagacagta
     5341 gttaaaatcg aacagttaga tgtaatacgc aaacaaaatg attatacgta tgatattgaa
     5401 gtagatggca agattgataa tcaaacgatt aaagtttgtt ctaaagcagg ggcggatatt
     5461 tttgtatcag gcggtttcat cttcaatggg aaaaataccc cgcaagaaca aattgataaa
     5521 ctaaatcaat cattagagga agtgaagtaa aagatcatga ccaataaaga ccaaaataaa
     5581 aactatttag aagcgattat cttagaatta caaaataact caaaacgtat taattctgta
     5641 gagttagaac aatttagtga gaaaattgtt caagctaaac acatctttct agctggtgca
     5701 ggacgctctg gaattgccat gcaagcattt acgaatcgct taatgcattt aggtttttca
     5761 gtcagccttg tcggtgaaat tagttcacca cattcgcaac ctggagattt attaattatc
     5821 tgttctggct caggtgaaac aggtagttta aaaaatttag cggccaaagc aaatcaaagt
     5881 ggtgtaagca ttgctttagt taccatgaaa aaagactcgt cgattggtca attagcagat
     5941 tctgttcttg tattaccagg tattaccaaa gatgaaaatc aacgagaaga agatactttt
     6001 tcacaaccta tgggttcaag ttttgaacaa ttagcatttc tcacctttga tagtttagtg
     6061 ttatctttaa tggataaaac aggtgaaagc tcagaaacga tgtttgctcg acatgcagat
     6121 tttgagtagt agattttaaa gataaaaaac cattctctaa atgctaatta ttcagcaaat
     6181 atgagaatgg tttttttatt tgattagaca taacttaata attcagtagt tttgagcgta
     6241 agcgtgtcat agaatggtac ctaaacacaa atagcccgat ttgatacact caaatcaggt
     6301 tatttgtggc ttatattatt tacagatttc ttgtatctgc tgattccatg gcaaataagc
     6361 gcgtagcgct tcaggtgtca tcgaagacag attgggaatc tctgtaaaaa gatgatttaa
     6421 atatttgcgg atgtttaacc cattcgcaac agctgtctgg ataatactca atataattcc
     6481 atttgatcta gcgcctttta agcttttaga aaatagccag ttttttcgac cgatcactaa
     6541 ctctttgatg gcacgctctg ctcggttatt tgaaagttcc aagcgtccat caagcaacac
     6601 aatctgaagt ccgtctttat gattgactgc gtattgaacc gcctttccaa gctgcgattt
     6661 tggcaaataa gcaaaactct cacaccaatg aaaaaattca gtcatcacag gctgcaacgt
     6721 ttccttcctc gctacaaacc tttcttctgc gctaagctta ttaaaggtcc gttcgattcg
     6781 aaacatcttg tcacagaagt ctaatcctat tttggcatga ctttttgggg acggttgttt
     6841 acccaatgct tcaaaaaact ttctgcgaac atgcgcccaa caataaacat tgtttaccgg
     6901 cagctttcca taggccgcgt aaccatcagt atgcaagaaa ccgctaaatg attctaaaaa
     6961 actttttggc tcatcaacgg aacgcgaatt tgcgtgctcg tagagcacaa cttgtttttc
     7021 ttcgctttta cctgaagcat ataaccagta aaaggtcttt tctttatgag tattcaatac
     7081 ccgatagctt gtctcatccg cgtgtaagac ctcctgattg cttaattctt gatgaaggca
     7141 attgtagatt ggctctaaaa ccatttcact taccttgata tgccaattag tgattttttt
     7201 acgatcaaga gggaaaccaa gtgcatgcca ttctttttct tgtcgatatg ccggcacctt
     7261 tttaacaaat ttttcccaaa tactctgtgt cacaattgaa gaggaaccta gactattatt
     7321 gatcaacggt ttaggaacag gcgctttttt gatttttgtt tctcctaatg cttcacattc
     7381 tctgcaatga taggcatgtt ggtagatgac ttgtttttct atatgggcag gaacatacaa
     7441 tggctcttct ctaagccttg tcgcaccgat atcatgtaaa gtatgaccac agtcttcaca
     7501 caaacaatcg gcacctttta attcataatg aataggaact tcaggcagac cttctaatga
     7561 aagtgctttg agtcccttgc gttttctgcg tttaaaagaa gataccgtaa tgggttcctg
     7621 gctttcttgt ccagtgtgct ctggttcatt aaaagttgag tcgtctaata aatcgatttg
     7681 cccatctacg ggtggtgttt ctttttttcg tccaaacaag cgtcgattca tttcagcaat
     7741 ttgttctcta agaagagtat tttcctgttg ctggatttca agcaactgtg ttaattgctt
     7801 gatttgtaca agaagttcag attccatttt tgtcattttt acaccaccat tctagcgatt
     7861 ttcttacatc tattatacca ttttatgaca acgaaaaaca gacaaaatcc ttgatttaac
     7921 aaggttttcg tctaatatat cgcacctttt ttcgtttctt ttagcgtaca tttttcttcg
     7981 attgaaaggc cagtcagtaa gacttctttt ctggaacgcg gccattgaag gtacccactt
     8041 tcaaatcttt tgtacattaa tacaaatcca ttttgatcct agtaaagagc cttgaagcga
     8101 tctttcttcg taccgcaaaa taagaaaagc gcttgatcgt aaacatcaag gtcatattct
     8161 tctataacga aagctgctag tccatcaatt ccttttcgaa gatctgtttt tccacaaacg
     8221 ataaaaatat gtttcaatgc agttgcatca atgagcattg gcattcaact cttttaacaa
     8281 ggtatacaaa agatatttat ccattccatt ataaataaat atttccttat cttccgattt
     8341 gattctgcaa gctagttttg gcttggtgtc aatttgcgat ttttgagccg cacttgaaaa
     8401 ttgaactggt acaatatctt ttttattcat tcgtttttct ccttctatat ttgatagttt
     8461 aattatagaa gaaagataat cggcgtacga gatactatcc tatgacacgc ttacatttaa
     8521 tttaatattt tgcctcaaat tagccacact ttttatagaa ctaagaggaa aaatgaagtt
     8581 ttcttcctat taatatagtt gtttgaaatc ttaaatatag cgttttacga tgtaatggga
     8641 agtatggtga ataaatcaac tatgttcgta cgaagcattt actcaactaa tttaatcgaa
     8701 tctttcaata aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag
     8761 tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc
     8821 agtcataaag gtttccaaca ggccgaaggc gagctcgaac aaatgctgag tcaaccgatg
     8881 gagaattaga aacaatctac agtagggaag aaccattgac acaaaattat tgacgctccc
     8941 aactaaaagt gtcaaaataa tgatgaaatc atttagttct tataaattag aaaagagaaa
     9001 ataaatattc catatctact ttattggatc cgtaacatga ctaaattctg aatacttttt
     9061 aaagcttatc atttggtaaa actgaatata agttaattat atagactgaa aattttgaat
     9121 tttcagaata gaataactta aaagtactga caaaattttt taaaggggca atattaatgt
     9181 tctgagataa tcaaatccta ccgaaaatat caaaaatcca atgtgacaca atcagattgt
     9241 gtctcattcg agggaatcac acaatcccac gtggctctag gcagaattga atttttgctt
     9301 ggggcttttt ccttatattg attttgaatg agacttatta tatgttataa tgcaattaag
     9361 tatcattgga gagggcgaag aaaatgaaga cggtagaacc tattcgagac aaaaaaaaga
     9421 tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg ccaacgaaat ttggttcttt
     9481 tttcgattgg gatcaacacc gcctatcgca tttctgattt gcgtcaattg aagctggccg
     9541 acgtgctgga aatttcacgc ggtcgcgtgg tggtcaaaga tcgattagcg atgaaggaac
     9601 aaaaaacagc taaacataat tcggtctata tttccaacaa attgcgaaaa acgattttgg
     9661 agtacgttca acaggaattt cctgatcaac tccttcagca agattttgac ggttatttgt
     9721 tccctagtcg aaaagggacg aatgaaccgc tcaaccgcca aagtctgtgg cgaatcattc
     9781 atgatgctgg cacggcaatc ggattgaaag acattggccc acattccatg cgaaaaacgt
     9841 tcggttactt tctctataaa caggggacca aaacagaaat cattcagtcc ttgttgaacc
     9901 attcttctca gcgcgaaaca ttgcggtata ttggtattac acaagaagac aaagatacag
     9961 ctgtaaaaag cttagattta taatataaaa gttaagttta aacatggata gttttaaatt
    10021 agtagattga gtgttgggag gaaagaatga gtacattata tattatagga aacggttttg
    10081 atttaagtca tggtttaaaa actagataca ctgatttttc aaaatgggtt gaaaaaagtc
    10141 atagtgaaat attcgataaa atagacacaa tgataaatga aaataatgag gtgagaagta
    10201 cagctgatga taagataggt tggtttaatt ttgaaagtag tttgcatgag ttaattcaaa
    10261 gtccagtctt tgaagattat gtagaagagt ctaaggatta tctcataggt tatggacatg
    10321 aagattggtc agattctgcc catcatgatt ttcaaaatat ggtttatgat tatgttgttt
    10381 ctctagaaga attacaacaa cttttcaagg aatggatacg caaagaaata cagccgacaa
    10441 tagaacataa aattccaaaa taccaatttt cgtccgatgt actattcttt acattcaatt
    10501 atacagatgt tttggaaatt ctatacaagc aaccgaaaga aaacatatat catattcatg
    10561 gcagttatga cgatataata atcggccact ccgagaattt tgacaacttt ataggagat
//
LOCUS       DACCVU010000107        10612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCVU010000107 DACCVU010000000
VERSION     DACCVU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.107"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            28..195
                     /locus_tag="IVW29_002316"
     CDS             28..195
                     /locus_tag="IVW29_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7839782.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(255..608)
                     /locus_tag="IVW29_002317"
     CDS             complement(255..608)
                     /locus_tag="IVW29_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7839783.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1112..1531
                     /locus_tag="IVW29_002318"
     CDS             1112..1531
                     /locus_tag="IVW29_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7839784.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1557..1763
                     /locus_tag="IVW29_002319"
     CDS             1557..1763
                     /locus_tag="IVW29_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839785.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1991..2164)
                     /locus_tag="IVW29_002320"
     CDS             complement(1991..2164)
                     /locus_tag="IVW29_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7839786.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2140..2805
                     /locus_tag="IVW29_002321"
     CDS             2140..2805
                     /locus_tag="IVW29_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7839787.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(2887..4053)
                     /gene="dnaJ"
                     /locus_tag="IVW29_002322"
     CDS             complement(2887..4053)
                     /gene="dnaJ"
                     /locus_tag="IVW29_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7839788.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4204..6033)
                     /gene="dnaK"
                     /locus_tag="IVW29_002323"
     CDS             complement(4204..6033)
                     /gene="dnaK"
                     /locus_tag="IVW29_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7839789.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6084..6647)
                     /gene="grpE"
                     /locus_tag="IVW29_002324"
     CDS             complement(6084..6647)
                     /gene="grpE"
                     /locus_tag="IVW29_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7839790.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6741..7784)
                     /gene="hrcA"
                     /locus_tag="IVW29_002325"
     CDS             complement(6741..7784)
                     /gene="hrcA"
                     /locus_tag="IVW29_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7839791.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(8018..9183)
                     /locus_tag="IVW29_002326"
                     /pseudo
     CDS             complement(8018..9183)
                     /locus_tag="IVW29_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(9199..9426)
                     /locus_tag="IVW29_002327"
     CDS             complement(9199..9426)
                     /locus_tag="IVW29_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7839792.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9910..10500
                     /locus_tag="IVW29_002328"
     CDS             9910..10500
                     /locus_tag="IVW29_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7839793.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         3037 a         2161 c         1850 g         3564 t
ORIGIN      
        1 atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta
       61 aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa
      121 gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag
      181 acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt
      241 atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat
      301 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt
      361 cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt
      421 aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta
      481 gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt
      541 ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt
      601 ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat
      661 agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca
      721 ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc
      781 caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc
      841 cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca
      901 atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc
      961 tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc
     1021 aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac
     1081 ttcctttcct agacagaaag ggggtgagat tatgaggaca tcttctttcg agcatatcgt
     1141 cagattacag ttcaattctt taatgctgat tgtcattaag aacaaagtga aaagtcgaaa
     1201 cagacagctt gcaagacatt ctaaacgtga agtattgttt tgtgaaatag ccgaaacgaa
     1261 acaagttgaa cgtggaacga ttgatacctg tttttatgat tgtgtatcat ttaaagtctt
     1321 gcactttaca atttatgtat ctgatgaaac acttggtaca gttttacata gactatcaga
     1381 aaaacagcgt agtgcgattt tattacgtta ttttcaagga atgaacgaca gagaaatttc
     1441 agaactgtac catgtatcac gttctgctat ttgcagtaga agaactagag gactaaggaa
     1501 gttaaaaatg cttttgagcg aaaggaagta aagattatgg ctatccctct tttgaattga
     1561 tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga gattttgaag ttttatgacg
     1621 gttatatttc taaattatgc ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta
     1681 tgcaggttga tgaagaacta aaaggagaaa ttcatacaga catgatgaaa gcaatattga
     1741 aatttgaaat cggagtgaag taatcaactg tataaaaaaa tcgggagata cacagtttcg
     1801 tgttatctcc cttagtagtt ttacggtcat atcaaggctc attcaatcgt ggtaaattcg
     1861 tggtaagatg agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat
     1921 aattgaaaat tagatataaa atttaataaa aaccaaaaat gcacggtcgc catgaaggga
     1981 ccgtgtattt ttatcttctt attttctcac ctaatcgtat cagccgcatc gcttgttttc
     2041 ctgcacaaac aagcaatcgc tctacctgtt ccatcgcata agaaagtacc atcggttcat
     2101 tgatgatatc aagtacatag tcgataccac cctcacttaa ttgagcttga tttttcttct
     2161 ccatattatg tagttgtggg acaactttct gacaatcagc aattgtgtca agaacggatc
     2221 aatcctttat tggagttgct cgaaagggtc ttctcttatt acggacaggc tttgctcact
     2281 gtacatagac aacaaatcat tatcctagtc aatcggattt ccagagcctc attgctatca
     2341 ttgcttgata aaatccagac aaagtttaat aaaatgtatc agttacagct aaactttgga
     2401 attgggtcgc tttgttatac agagcaagag acaccgcaat ctttccttca tgccaaacaa
     2461 gtttgcgaat ggatagcttt ccatcaatct gtgaacgaga tccgcttttt tgaagattta
     2521 gacctcggga tcgttttacc agcaatcccg tcagatcaac gaacattata tgtaaaacgt
     2581 attttgaaaa gcctgacaga agaagaagta catttgttca agaagacttt ggcatgcttc
     2641 agtaaaaata acggaagcat caaaaattgc tcagaagaat tatttattca taaaaacacc
     2701 ttgcaatatc gattgaacaa gttccattct ctcactggtt atacccctag aaactatgac
     2761 gattatcata ttctaaagct ggcttttttg ttggtacaaa catgacaact acaaaaaaac
     2821 caggcgaaca tatgattgtt cgtctggttt ttaattatta atgacttagc gtttttacta
     2881 gaaaaactac ttccgctttt tcccgccaaa agcatcttta aacttatcga agaaaccgtc
     2941 ttcttgctgt tcttcaactt tgatgccgcc agcttcagcg aatgcacgta atgcttctct
     3001 ttgttgttca tttagatttt tcggtgtgat cagttttact ttgacatgtt ggtcgcctgt
     3061 tgcaccacca cgtaacttcg gagcgccttt tccacgtaaa cggaagttcg tacctgtctg
     3121 tgttccagca gggatcttca atttcacatc tccatggaca gttggtactt ggacttcatc
     3181 ccccaaagct gcttgtacaa aacttaaagg caattcatag taaatttcag aaccttcacg
     3241 atcaaagata tcactttctt ctacacggaa gaccacatac aaatcaccaa atggtccgcc
     3301 gttttctcca gcttctcctt gaccagctaa acgcatttgt tgtccttctt ctacaccagc
     3361 tggtacattc actttaactg aatgtgcttt cttctcatgt cctgagccat gacaggttgg
     3421 acaaggttct ttgatttctt tccctgtccc atgacaaaca tcacaagttt gacggctcat
     3481 cactcgtcca agtggtgttt ggcgttctac attaatcgta cctgcaccat gacatttatg
     3541 acaagtagtt ggttgtgtgc ctggttttgc accgttccca ccacaagtat gacagatttc
     3601 ttcccgatta tacttgattt ctttttctac accaaaaatc gcttcttcaa aagacaagtc
     3661 gaccgtatat tgcaaatcag caccttgacg aggcgcattt ggatcgactg accgaccgcc
     3721 tcctccaaag aaagagtcga agatatcttc aaaaccaccg aagccgccac cagagaagcc
     3781 gccgaatcca ccaaatccgc caccagctcc accagcaccg taatttggat ctgtgcctgc
     3841 atgcccgtat tggtcatatg cagcgcgttt ttgcgggtca cttaaaatct catatgcttc
     3901 tgatacttct ttgaattttt cttccgcatc aggttcttta ttgatgtcag gatggtattt
     3961 tttcgaaagc ttacgataag ctttctttat ttcatcttct gatgctcctt ttgagagccc
     4021 taagacttca taataatcac gttttgttgc cataggcttt cctcctattg gttctcatca
     4081 ttttcacgtc gcttgttatc atatcatatt ttgtttgaaa caaaaaccga tacttcaaac
     4141 tatttttcaa aagaaaaagc caaagtagca actttggcct tctctcgaaa ttcagtgaac
     4201 tgattattta tcgtcgccat cgacttcttc aaaatcagca tcaacgacat catctgctcc
     4261 gccttgagct gcttctgggt tttcttgagc ttgttgttgt gccgcttgtt cgtatagttt
     4321 aacagtcaag ttttgaacga tttcgttcaa tgcatcgcgt ttagttttca tttcttcgat
     4381 gttgtttgct tcaacagcgg ctttcaattc atcgcgagct gtttctgctt ttttcacttc
     4441 ttcttcgtcc actttgcctt ctaattcttt caatgttttg tccacagaga atagcaatgc
     4501 atctacatca ttacgtaggt caacttcctc tttacgagct ttatctgctt ctgcatttgc
     4561 ttctgcatct tttaccatac gttcgatttc ttcgtctgtt agacctgaag aagatttgat
     4621 cgtgattttt tgttcttttt gtgtacctag atctttcgca gatacattta caatcccgtt
     4681 tttatcaata tcgaatgtta cttcgatttg tggaatacca cgaggagctg ctggaatatc
     4741 tgtcaattgg aatcttccta gagttttgtt gtcagcagcc attgggcgtt caccttgtaa
     4801 tacatggatg tctactgctg gttgattatc tgccgcagtt gagaatactt gtgatttact
     4861 tgttggaatc gttgtattgc ggtcaatcaa tttagtgaac actccaccca ttgtttcgat
     4921 acctaatgac aatggtgtaa cgtcaagtaa aacaacatct ttcacatcac cagtgatcac
     4981 gccaccttgg atagcagcac ccatagcaac tacttcatca gggttaactg atttgtttgg
     5041 ttcttttcct gtttctttac gtactgcttc tacaactgca gggatacgag tagatccacc
     5101 gactaaaata acttcgtcga tttctgattg tgataaacct gcatctttta gcgcttgacg
     5161 tactggtact tttgtacgtt ctactaagtc agctgttaat tcatcaaatt ttgcacgtgt
     5221 caatgtcatt tccaaatgca atggtccaga ttctcctgct gtgataaatg gcaagctgat
     5281 ttgtgtgctt gatacgccag ataaatcttt tttcgctttt tcagcagcgt ctttcaagcg
     5341 ttgtaaagcc attttgtctt gagacaagtc aatgctgttt tcttttttga attctgctac
     5401 catgtagtcg atgattttgt tatcaaagtc gtccccacct agatggttgt cgccagctgt
     5461 tgacaataca tcaaagacac cgtctcctag ttcaaggata gatacgtcaa aagtaccgcc
     5521 accaaggtcg aatactaaga ttttttcgtc gcgatctgtt ttatctaaac cataggcaag
     5581 agcagctgca gttggttcat tgacgatacg ttctacttct agaccagcga tcttaccagc
     5641 atcttttgtt gcttgacgtt gtgcatcatt aaagtaagct ggcacagtga taactgcttt
     5701 ttcgactttt tcacctaaat agtcttctgc gaatcctttg atatattgaa ggatcattgc
     5761 tgagatttct tgtggtgtat atgatttacc ttctacatct actttgtaac ctgcttcacc
     5821 gatatggcgt ttgattgatg aaatcgtatt tgggtttgtc acagcttgac gttttgcaac
     5881 ttcccctact tggatttctc catttttgaa tgatacaaca gaaggtgttg tacggtttcc
     5941 ttctgggtta gcgatgattt ttgcttcgtt tccttccaat actgctactg cagagttagt
     6001 cgtacctaag tcaataccga taattttact cataattaac gtctccttct ttaattgaaa
     6061 gtgtaagttt tatatccgca caattattgt gcaacaatga ccatggttgg tcttaacacg
     6121 cgatcatgta atttgtagcc ttcttgtagt acttctacaa tcgtatcggc tggtgtatct
     6181 tcagtcgctg gaacagtttg tactgcttga tgaagattag gatcaaaggc ttctccttta
     6241 gcagggattt tttcaattcc ttcttcttcc agtgcgttac gcaaactttc caagaccatt
     6301 tccacgcctt ttttcaaacc tgccccttga tcatctgaca cttctgtggc taatgcacgt
     6361 tccaggttgt caattgatgg aagtaatttt ttggctaaat cttgtgaacg gtatttttgt
     6421 aattgctcac gctcattttt tcctcgattt gccatatttg cgatttcagc acgagcccgc
     6481 aagtatttat cttccatttc ttctaactca gcttttaatt tcgctgtttc gaattcttcc
     6541 gcttcagctt ctgaaatacc cgaatcttct gcagcttcga catcgatttc cggttctggc
     6601 tgagcatctg tcatttcttt atctagttct tcttgatttt ctttcacgat acagtcttcc
     6661 tttcattatt cagtcagctg tttgattgct cttactaaga aagagagaac gatcagctgc
     6721 aaatagcttc gttattcaca ttacgagttt gacaagtcaa gcgaacggta gtaatcagca
     6781 agtttagctg ctagttctct tcgataaaca tcgactagac cgaacatccg agaataaggc
     6841 atactagtag gccctaacaa ggctattgta cctcttccat gaccatctat ctcgtaactt
     6901 gcttgaatca tactcatatt ctgcaataaa tcattgccta attcagcacc aatcttgata
     6961 tgaatattgc tatccatcgg agtgatcatt tgtgtaagtc cgtctgagtc tttcataaaa
     7021 gaaaacatcg atttgaactg atcaagatct tgacggggct cataatccaa gagattcatt
     7081 tgtccactga cataaacctt atcttcaaaa acatgaccaa gaacggtgtc aaataactga
     7141 gacatccctt ctgtcgtttg gaaatattta tgaagaatca tcggaatctc cgtacgaagt
     7201 ttatggtaca ccgtcatcag aggatctccg actagacggt cgtttacaat ctgtaccatt
     7261 ttttctagtt cctgacttcc catatattcg ggaagagtga acacttggct ttctacattc
     7321 cccatatctg ttacgacgat agcaataatc tgtcgattat tcaagggtac gatacgaaag
     7381 ccagtcagtc ggcggtcttt tatttcaggt cccaatgaaa aagtcgtata gcttgtcagc
     7441 tcagataata tctcagcaga ttgcttaata atctcattga tttcatgaaa ttctctgcct
     7501 agagactgac gaatcagcag caaatcatct tctgctacgc gagcaggttt taacaaatga
     7561 tccacgtaat aacgatagcc taaagcagat ggaatccgcc cagaagaaga gtgagtcttc
     7621 aacagcaatc cttcatcttc taatgctttc atatcattgc gaatcgttgc tgagctggcc
     7681 ttgactccct cagacatcaa tgttttggaa ccaacgggca cgccactact cgtatagtgt
     7741 tggatgatca gacgtaaaat gtcgttttgt cgctttgtta acatcttcct cacctctcat
     7801 tagcactcat atattctaag tgctaatcca cttataaata tagcaagaag cccttctcct
     7861 gtcaagagaa agactccatt ttttagcact ctaaacacaa gagtgctaat tctaaataat
     7921 tgatgtaaat ccagttatat caacgtttca atagtccttt attgtatttt tgaacttttt
     7981 ttatttaaat tcagataaaa aacaaatatt ctttaaacta tttttcaaac aaaaagcgct
     8041 caaacacatt gttccccaaa aaggtccctc ttggtgtcaa ataaacacgg tcttcttcat
     8101 taacaagtaa cccttcttgc tctaattccc ttagcacatc accgtacacc gattcaattg
     8161 ttttttgata gcgatcatag aaatgttttt tagtaatccc tgtttttttc ttagtcctaa
     8221 gaacatttct tcttctattt gattttttcg tgtcaatttt tcttcttcca ataccgggcg
     8281 ttcatttgct cgtaatgggc gtaaatagtg ttggattggt cctttgttgc ggtaacgaat
     8341 atctccaaga taaccgctag caccagctcc aaacccaaaa tagtggtcat tgttccagta
     8401 catgaggtta tgttgagatt catgtccgga gagcccaaag ttacttattt catattggtg
     8461 acgtccatgc ttttccattg cttcaatcgt ctcttcaaac attctggttt ccgtttcctg
     8521 atcaggcagt tcgagtcttc cttgtcgtac ccagttcatg aacattgttt tgttttcgag
     8581 gatcaatgag tacaaagaat agtgaggcag atccaaagcc aaagcttttt ccaaagtatc
     8641 cagatatcct tctagtgttt gtcccggtaa tgcataaatc aaatcgatgc ttacattggt
     8701 aaaattttct gcttctaaaa attgcatagt ttgatacaca tcttctgcgg tgtgtttacg
     8761 tccaattttt ttcaataagc gattatcaaa tgtctgcact cccatggata aacgattgac
     8821 gccatagttt ttcattactt gaagtttttc acgggtcaaa tcacctggat tcgcttctac
     8881 agtaaactca ttaccatttt gaaaaggcag gatctctcta gcacctgata acagtcgatc
     8941 aagttgttta gcagataaag aagttggtgt cccgccccct acgtaaagtg tttctaaatt
     9001 cgctgtcgga tgactttcta gcattatttt catttcattc agcagcattt ccacatactc
     9061 atctactggc tgcccttcta aaaaaacttt attaaaatca cagtaatagc agatatgctc
     9121 gcaaaatggg atatgaatgt acgcagaaac cttgttttct gcgggattta ttgtttctat
     9181 cattgaaatt ctttctcctt acgtcgaaaa gtcgtgcctg ttttcgacct tcttaaaatc
     9241 gcgttcaagc accaactcat tgaaataagc cgcatattta gaatcaagga aacctagctc
     9301 taaaaatgct tgcttattgt catcggagct acacggtgct tttacagcct ctcgataaac
     9361 ggtaatcgca aagctgacct tcggcattaa gtcaaaatct gcaaaaatat gaaaagctat
     9421 tttcacagat tcctccttaa gtgctcagac cacaacgctt tgcttacaac ctctcatata
     9481 cgcatactaa acgtatttgg cgaacaaatc cactaaaaaa tccaagaatc gacttcttgg
     9541 atgatttttt ctttcattaa actggttacc tagttatata tctctagggt tgttttcatg
     9601 tctgagtgcc cgatatgatt tatttttcgt ttctataatt actttttgcc ttccagtttc
     9661 ttttaatatt gtactcaagc aagtgggaat tggttctttt gtcatccatg tatgcaaaat
     9721 gttataatgc tggtaaacaa aaatgattac ctgtcatttt tatttagttc gtatttaatt
     9781 aagttgacta tctgtatact ctgtctaaaa cttaagaatt gttgttttcg ctgtcgctac
     9841 ccaattttaa tgaatgtctg taggttaaaa ctgttgtcct tgttatagat agtaaaggag
     9901 agcttgaaaa tgaaaactgc gattacgttt ctattagagg aatatgccga ttgggaaggt
     9961 gcttatatta ctagcacact aaaccaaaat gagaaatggc aaaacaaaac tgcttctatc
    10021 caatcagagg tcacttccat tggaggatta aaaacaaaaa tcgattactt aatcgaacaa
    10081 atacctgaaa aagttgacct ttttattatg atcggtggca attcatggga cataaaaaat
    10141 catcatttat atcaaataat cgataaattg ttaaaaacag acgtaccagt agctgctatt
    10201 tgtggggcag ttgattattt agctcagagc ggattattgc aaggttacaa acacaccgga
    10261 aatgcgcaat ttccttggtt agaatcggaa aattatcaga ataaagaaga ttttatagaa
    10321 caacaagctg tgaaagatcg taatttgatt actgcgaatg gaacagctcc tatagaattc
    10381 acagacttga ttcttcaagc agtccatttt gaaacagaag aaataagaga aaaaacgatt
    10441 cgtctataca aacttggatt ctatgattac tataaaaagt acggagatcc tttctcctaa
    10501 tcattctctt caattaaata ctccttatca atagtgtgac aagtaatttt actgatgatt
    10561 atttgtcaca ctttttctga catttatcaa agaagataca ctctatttat at
//
LOCUS       DACCVU010000108        10249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCVU010000108 DACCVU010000000
VERSION     DACCVU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.109"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            106..2802
                     /locus_tag="IVW29_002329"
     CDS             106..2802
                     /locus_tag="IVW29_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7839794.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2879..3367
                     /locus_tag="IVW29_002330"
     CDS             2879..3367
                     /locus_tag="IVW29_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7839795.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3379..3873
                     /locus_tag="IVW29_002331"
     CDS             3379..3873
                     /locus_tag="IVW29_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839796.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3894..4703
                     /locus_tag="IVW29_002332"
     CDS             3894..4703
                     /locus_tag="IVW29_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839797.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4700..5521
                     /locus_tag="IVW29_002333"
     CDS             4700..5521
                     /locus_tag="IVW29_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7839798.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5572..7671
                     /locus_tag="IVW29_002334"
     CDS             5572..7671
                     /locus_tag="IVW29_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7839799.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7668..8786
                     /locus_tag="IVW29_002335"
     CDS             7668..8786
                     /locus_tag="IVW29_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7839800.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8825..9703
                     /locus_tag="IVW29_002336"
     CDS             8825..9703
                     /locus_tag="IVW29_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7839801.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9894..10046
                     /locus_tag="IVW29_002337"
     CDS             9894..10046
                     /locus_tag="IVW29_002337"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839802.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3158 a         1678 c         2286 g         3127 t
ORIGIN      
        1 tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat tattttgtca
       61 gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga atacaaatat
      121 agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa actggaggaa
      181 aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact aacagggaat
      241 atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg tcagaatgcc
      301 agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc gggaaagcct
      361 gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca aataaacgaa
      421 gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca aaaatgtata
      481 gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc cacgggtgtt
      541 ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca aattattgaa
      601 aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc cgaactgctt
      661 tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa ggataccgat
      721 ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca tcgcctatcg
      781 aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg tccagtcggt
      841 gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac ggaaaaaggt
      901 gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat gttgccaact
      961 tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt tcaaaatgaa
     1021 gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt aatgttatct
     1081 catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag ttgtgcggat
     1141 gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat cttgattcag
     1201 ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt gataaagaaa
     1261 aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat tttggaaaaa
     1321 attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat aaagaatgaa
     1381 gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag tagaaaattg
     1441 atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta cggattgacg
     1501 acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc aagctataag
     1561 acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac gacttactta
     1621 tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa ctttctaaga
     1681 ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct gaagggcctg
     1741 ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa tcaattatgc
     1801 ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc agagattgtc
     1861 gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt gctgatcgta
     1921 gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa aggggagtta
     1981 cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa gatgacgatg
     2041 aatgcgccat ttaaacagat tgcggaggat gcagcagaca agtattcaat caatgttcaa
     2101 tactttgaag gcttttctca aagtagcaac attatcattt cctgtatgtc gggtttgggg
     2161 atttcggaca aattgaaaga tgtttttcgt catcatttat cggatgaatc catcgaagtt
     2221 ttcacgaagg actacagaga actacgtgaa ttaatcgacc aaaatgatga aacttatttt
     2281 gacaagacaa aattggtcat cacgacttcg gatcttccac gttccttttc aattcctaat
     2341 attaatgttt atgacatctt agacggtgat ggagcagcca atttggagca tattttgagt
     2401 cctgaaatga agccggcaga atttaaccgc ttgattcagg agctagtaaa attcttttcg
     2461 ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat gagtgaagtg
     2521 gaaacagtca tttctaaata tgaaaattac tatcatttca ctttaaatgg ccgtgtaaaa
     2581 ctaaatctct atatgcatac ggcattgatg atggaacgtt tattcttggc tcggacagaa
     2641 aaatctgaag aaccgaatta tgatttgaaa ccagatgaag atgaatttta ttatgtttct
     2701 agaagtattt ttcaatcaat ggagatgaaa tataatttta aagtcaatgc ttatgagctt
     2761 tcgctgctct atgaattatt aggaccgtat attcaaaaat aaagtgtcta aaaaagtgtc
     2821 taatctgttt tttttaggtt agacactttt tttaatgtga attaacgtga ttaaaccaat
     2881 gtttttaaaa gttggcacag gaattgctta tataaaagtg aacagaaaga aaggaattaa
     2941 ccatatgaag aaaattctaa tagcgactca cggtcactta gcttcaggct ttcttagcag
     3001 tattcaatta ctgacgggga aggttcaaga gattacggtg attaatgctt atgtggatga
     3061 gtgtgatttt gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc aggatcagat
     3121 ttttgttttt accgatttat ttggcggaag tgtcaatcag aagattacca aaaccttttt
     3181 ggagaaagag attgctgtaa ctattttgtc tggctttaat ctgccaattc tgctcgaaat
     3241 agtattggca accaaggaat taacaaagga ggatattcag gaactggtaa ataaatgcca
     3301 acaggaatta aagatgacca ttcttgatga ggtacatgta gtaaatgaag aggaagattt
     3361 tttttaggag gaagaaaaat gattaaagca gtgcgaattg atgaacgatt aattcatgga
     3421 caagtagcga tgacttggac aaagagtcta aatttaaccg ggttagtagt agcaagcgat
     3481 gaggcggcta ccaacgatat tcaaaagatg acattgaaga tggctgcacc atcgaatgtc
     3541 aaagtcatta ttaaaacagt tgatgacgcg attcatttgt taaaagatcc gcgggcgaag
     3601 gacatgcgct tgatggtttt agtccctaat gtcaaagatg cggtaaaagt tgcgaaagct
     3661 ttcccaacgg aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac accgccatcg
     3721 gaagataaga aaaccttatc aaaggaagtg atgttaacga cgtcagaaat tcaagactta
     3781 aaagaattag ttgaacttta tccagatacc gtttttcagc ctacaccggc gatggaaaaa
     3841 cgttcagcta tctctatttt gagtgatttt tagaaaaagg aaggagaaaa attatgtatg
     3901 atgcatttgt agtcgctttg gccgttttta ttggagttgc tggacatgaa atgttcggga
     3961 tgtccatgct gagtcgtccg attgttgttg ctccgttagc aggagcgttg atgggggatt
     4021 tgcagacggg gttgattgtc ggggcgtcac tagaagccat ctttatgggt gtcgtcaata
     4081 tcggaatctc tagtacggcg gagccttctt tggctgctgg gctggctaca gcgttcgcaa
     4141 tcaaaatggg aggaaacgtg gatgcgatta ttcccattgt ctttccacta gcagtattag
     4201 gattacagtt aatgaatatg attttttctt ttgtatgtgg accgatggct gctaaatttt
     4261 tcaaatttgc aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc tttatgtggt
     4321 ttgtccacta tgcgctttat gctcttattc cattctttgc cgttttattt ggagcagatg
     4381 ttgtcaaaag cgtcttggat aatattccag aggtcatcat gaacggatta acagtcgctg
     4441 gaaatctgct gccggcagtt gggatggcga tgctgttacg gatgctttgg agtaaaaata
     4501 ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta ccattgattg
     4561 caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat ctacaactaa
     4621 aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg cttgaccaag
     4681 aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg agaaaaaatt
     4741 ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc cggtgacgat
     4801 gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct acaaaagtga
     4861 agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga ccattccttg
     4921 cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg aaaaagctgg
     4981 ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat tggctggaat
     5041 tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag cgatcaactt
     5101 agccaatgcc ggaaatattt tggcaccaat tatcttttta gttatgttca atatacctaa
     5161 ttacttgatg cgatactatg gcggcgtttt aggctattca ttaggctcta aatatattga
     5221 aaagctgtat gcgaacgggt taattgaaat tctgacaaaa gctgctagta tcttgggtct
     5281 gatcatggta ggtgcaatga cgatcagtat ggtcaaattc aataccgttt tgagcttttc
     5341 aatgaagggc gaagaactaa tgaatgtcca agaaatcctc gatagtattt ttaaaggaat
     5401 tgtaccgtta ttggtaacat tgagctgttt caaattacta gataaaaaag tcggcgtcat
     5461 caaaattatt tttggaatgg tcgtcttggg aattttattg tcattacttg gaatcgcata
     5521 gtaggaagct gcagctacta ggctgcagtt tttttagcaa ggagggaaaa gatgactatt
     5581 aatgaagtaa tttattcacc caaacaccca ttttttaatg ctgagttaaa cgacggaaat
     5641 gtgactactt caaacgaata cagctactat cggaatattc ctgatgtaaa gggattgacc
     5701 ttcgatgtaa gtaatcaaaa gacattagca aaccttgatg tttgcgggac gattcaaacg
     5761 ttgacctttt atcgagaaaa tttactaact gaggaaaaac caggtgtttg ggtcaataaa
     5821 cagctctctc aaaccaattc gttatcctta aaggttgaag taaacgggat tgtctaccat
     5881 ttgtcagaat cggatcatcg tattgaagtc gatgtgattc aagactgttt gcctagatat
     5941 attcatcatt attcgacgtt taaggtcatt gtagtatcct ttgctcccat actggctaat
     6001 aagcgtttaa gtatgcttgt tcaacaggtt tacattgtga atgaaacaca cgaaagcctt
     6061 caagtttcag tgaaagatgt gccttcgtat caagagaagt attcggatca gcaaaatgtg
     6121 ttgatttctc aaaaaggaaa taaggacacg atcgcacaac aggaagtggc aagcttcgcc
     6181 attgcttttg tggacccaaa tgcttttgaa gaagtaatga cttttcagga atctgatatc
     6241 gaaaaatggc tgaaggaaac attgaactat tttgctcagc tatttgggca gctgtcaatg
     6301 ccaaacgata gagttgtcca tctctatcgt cgtgcacttt atcaaagctt ttctagtttc
     6361 ggtatgaatc gacagggaca ggtcgtcggc agcaattggg gcagctatcc tgctactaat
     6421 cgaatttgga ataaggatat gtattacgca tcattgccat tcaccatgtt tgaaccggag
     6481 ttatgtcaaa aaacggttct atggtttgat caatatggcg ttaagtttcc tggttcaaaa
     6541 tttacaggag gtatcaatca ttctctgagt aattcgttat ccagtgcgtt actggctagt
     6601 ctttattttg aacatactgg ggatacaaaa ttctttgagc ggtgtccgca ggttctactg
     6661 aatgcttcaa gaattattga ggacatatta gctcaacgtc ggtcagggga accaatgctt
     6721 tttccatctg tttggttatc agacgccttt gcacttggaa agtatcatac cggcagcaat
     6781 ctttgtcttt ggaaggcctg tattggactt gcagaaattt ttgaagccct agaacaactg
     6841 agtcttagca agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa
     6901 atgactatcg atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa
     6961 acaacctatt cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta
     7021 agtgatgtga ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac
     7081 actacgttga tgccttttta tcaattttta gataataaag atcatttata tcagaacacc
     7141 atggcctttt ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact
     7201 tggggattag aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg
     7261 cacaaccctc aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac
     7321 ggttcgtggt ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac
     7381 tttggctgcg gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa
     7441 tatttcggat tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg
     7501 ttttcttgga agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc
     7561 gagttgagta tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt
     7621 cttcacgtag aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa
     7681 aaaatattac ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag
     7741 gcgagaaagg taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg
     7801 gcgcaattcc gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa
     7861 taattcgtca tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac
     7921 gtgatttaat cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt
     7981 taggggattt cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag
     8041 tggttaatcc tacagggggc tttaattcct atttcgaaat gcctttccaa acatccgcta
     8101 aaattacgat cgagaattta catccgaagg atatgtcctc atttttttat acgataaatt
     8161 acgctttggt ggacgaattg gaagaaaata gcctatattt tcacgcaaat tggcatcggg
     8221 aacgcattac caaattacag gaggattata agttaattga tcgattaaag ggtcaaggct
     8281 attacgtggg tacttacctt gcgcttacct gcctagaacg ttattggtgg ggcgaaggcg
     8341 agtttaaatt ctacattgat ggggatagtg agtatccgac cgtaacctca acaggttcag
     8401 aagattattt tggcggggcg tgggcctttc atgagtacga tgagttcgga cgtccacatg
     8461 cgaaaaacta ttcaacagcc tttttagggt atccttacca atcaaataga gatgccacaa
     8521 gagatcgttt tgaaacggga aaattgaatg ccgttcatgc ctttggtgac gacgcaatgc
     8581 cacgacatgg tctttatcgt tggcatcttc aagaccccat ctgttttacc gaagatttat
     8641 cggtcacttt acaacaaatc ggcaacgatg atgttcgctt atttgagcgg caagacgatg
     8701 ttgctagtgt cgcttactgg tattcaacag aagcttcagg aaatactaaa cgtcatttca
     8761 ctgatcgaga acgcttgccg cgttaatgaa gtgtttttca catattcgaa gggaggaacc
     8821 tatgatgata tatggggcaa ttgaagctgg agggactaaa tttgtctgtg cagtcggtac
     8881 tgaggaatta acgattttgg aaagagtcag ttttcccaca acaactccag ttgaaacaat
     8941 gaagcaggtc attgattttt ttaaaccttt taaggaacaa ttggcaggca tcggggtcgg
     9001 ctgctttggt ccaattgata ttcaatgcaa atcaacgagt ttcggtcata tcacatcaac
     9061 accaaaactt gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat atttcaatat
     9121 tccgattgaa tggacaacag atgtcaatgc ggcttgctat ggtgaatatg ttgctggttc
     9181 tggcaaaggg ttagcaggtg ttgcatactt cactgtggga acaggtatcg gaggtggtgc
     9241 cttgttcaat gggactttcg tggaagggtt cagtcatccg gaaatggggc atatgttgat
     9301 caagcgacat ccaaaggaca atttttcagg aaactgtccc ttccaccaag attgtttaga
     9361 ggggatggca gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc agaatttatt
     9421 ggctgacgat tcgttttggc agattgaagc cttttacctt gcccaatgtg catacaatac
     9481 aacactgatg ttttcgccag atagaattat ttttggcggt ggtgtaatga aacaagaaca
     9541 catgaagaaa aaagttcagg ataaatttgt tgagcttata aatggatatg ttgaaatacc
     9601 accaattgat agctacatta ttacgcccga attaggtgat aatgccggaa taatcggcgg
     9661 attagcattg gccagaaaag cggtaagaaa taagcaacca taattaatga atgtttcatt
     9721 gatactcatg tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag ttggataaag
     9781 cgaaaatgaa ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca ttaaatcact
     9841 gaaaggaaat gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat accttgagga
     9901 tattcactgg tggcgtacac ttaaatagta atatattgtg ttactttatg tctgtggtaa
     9961 tagttatagt tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc ataatccttg
    10021 gcttaatgtt tgaatttttt tggtgataga ttttttcagc cgtataaaat aaatcgtaat
    10081 tctattatag agagttgtga ttataaaaag atagcaagaa aaaaaaagaa agtttctata
    10141 cattttaaat attttattcc tatttctatt gctaaaaaag tagaaattct atatttacct
    10201 ttaactctcg cttctcttgc aaatgtagtt agcattttaa tcgataata
//
LOCUS       DACCVU010000109        10087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCVU010000109 DACCVU010000000
VERSION     DACCVU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.110"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(47..367)
                     /locus_tag="IVW29_002338"
     CDS             complement(47..367)
                     /locus_tag="IVW29_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7839803.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(515..2749)
                     /locus_tag="IVW29_002339"
     CDS             complement(515..2749)
                     /locus_tag="IVW29_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7839804.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3228..3494
                     /locus_tag="IVW29_002340"
     CDS             3228..3494
                     /locus_tag="IVW29_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7839805.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3494..5221
                     /gene="ptsP"
                     /locus_tag="IVW29_002341"
     CDS             3494..5221
                     /gene="ptsP"
                     /locus_tag="IVW29_002341"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7839806.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5352..6431
                     /locus_tag="IVW29_002342"
     CDS             5352..6431
                     /locus_tag="IVW29_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7839807.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            6448..7671
                     /locus_tag="IVW29_002343"
     CDS             6448..7671
                     /locus_tag="IVW29_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7839808.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7760..8647
                     /locus_tag="IVW29_002344"
     CDS             7760..8647
                     /locus_tag="IVW29_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7839809.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8733..8963
                     /locus_tag="IVW29_002345"
     CDS             8733..8963
                     /locus_tag="IVW29_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7839810.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9020..9985)
                     /locus_tag="IVW29_002346"
     CDS             complement(9020..9985)
                     /locus_tag="IVW29_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7839811.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3209 a         1785 c         2038 g         3055 t
ORIGIN      
        1 ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac acggctaatg
       61 tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat tcgattactt
      121 tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa tttttagcat
      181 cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt tcattatgtt
      241 gaggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg actagatggg
      301 catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt acatttacca
      361 atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt attataattt
      421 cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga cggcatttct
      481 caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct tcggctgatt
      541 ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta tccagttttg
      601 ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca tcttcgattt
      661 gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag cctaagtcaa
      721 ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct gccagcattt
      781 ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt gataatgcac
      841 tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag ttgtttaatt
      901 ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg tttgcttcta
      961 ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag ctgaccgttc
     1021 ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata tgcaaaacat
     1081 ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt ctccgtactt
     1141 tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa ccaatcaatt
     1201 tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt gaatcttgtg
     1261 aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca ccagttggtc
     1321 caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca cggtttcggc
     1381 gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga gctttcaagt
     1441 catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc actggaatac
     1501 ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt gtttcctctt
     1561 cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga taataagctg
     1621 ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct tcttgcagtt
     1681 ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta gaacctgttt
     1741 catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa cgattggata
     1801 aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag tcttcataac
     1861 gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca tctacccgta
     1921 caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac tcatttaagg
     1981 tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa atattaccag
     2041 catccatatt tccatcacca gcagaaccag caccgactat ttcgtgaact tcatctatga
     2101 ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata cgttcttcaa
     2161 attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga atgacttctt
     2221 tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa ccttcaacaa
     2281 ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg gttcggcgat
     2341 ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga tcgatatcac
     2401 cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct tctgtattgc
     2461 cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt ggtggtgttt
     2521 gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga tcgtccaaac
     2581 tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt tgttgatttt
     2641 tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca ttgacagtgg
     2701 catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg aatcattcac
     2761 gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg aggtaaaaga
     2821 ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc tattgaccat
     2881 tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa ttcgtttcat
     2941 atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag aaagcgacaa
     3001 gaaaaaggaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa aagaatccgg
     3061 aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa cgaaaaaaaa
     3121 tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt actaaaaaag
     3181 aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg gaaaagaaag
     3241 aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta ttagtacaaa
     3301 cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca gtaaacttga
     3361 aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt actattactg
     3421 cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg aagaaagaag
     3481 gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg gtgtagccgt
     3541 tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa cagtagaaga
     3601 tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg aagaattgca
     3661 gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag tatttgatgc
     3721 ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa atattaaaga
     3781 caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata tcggtatgtt
     3841 cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc gtgacgttgc
     3901 taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa tgatcaacga
     3961 agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat tagatcgcaa
     4021 ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg ctatcatggc
     4081 acgttcttta gaaattccag caatcgttgg gacaaaagaa attactgcta aagtcaaaga
     4141 aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc ctacagatga
     4201 acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag cagaatggga
     4261 aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat tagctgcaaa
     4321 catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag cagttggtct
     4381 gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag atgaccaata
     4441 tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg ttcgtacaat
     4501 ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa tgaatccatt
     4561 cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa tgttccgtac
     4621 acaaatgcgt gcattgcttc gtgcatctgt tcatggtaat ttacgtatca tgttccctat
     4681 ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag aaaaacaaaa
     4741 attgataagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca tgatcgagat
     4801 tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct tctctgtagg
     4861 tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac gcgtttctta
     4921 cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa ttgatgcagc
     4981 acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc aaacagctgt
     5041 tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat ctattcttaa
     5101 aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg ctgatcgtgc
     5161 attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat atgtaaaata
     5221 aacaacgtat caaaagctgg tgtggcttgt aaaagagcta cgccagtttt tttgttcttt
     5281 tttaagaaaa cgtttttgtc agtaagaata gacaaaaata gggcttaagt ttgagtgaca
     5341 gtttgtaggc tatgttatac tatataagtg aagggggacg taaggtgaag gtattattat
     5401 attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg gcgtgcgctt gatcatcaaa
     5461 aacgtgctct gacggaagtc ggtatctctt atacactaga tgaaaaagaa gattatgaca
     5521 tcctgcacat caatacatac ggaatcaata gtcataatat ggtcaataaa gccagaagag
     5581 aaggaaaaaa agttgtctat catgcgcatt ctacagagga agatttccga aattccttta
     5641 tcggatcgaa ccagctttct cctatcgtaa aaaaatatct ggtaggattg taccaaaaag
     5701 cggactatct gatcacgcca acaccttatt caaaacagtt gttggaaagc tatggtatcc
     5761 gtgtgccgat tcaagctatt tccaatggga tcgacttaga gaaatatcac cctgatccaa
     5821 tgaaagaaca aaagttcaga gaatatttca aactgtctcc ggatcaaaaa gtaatcattt
     5881 gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt cgtagaaatt gctaagaaga
     5941 tgcccgaata cacatttatt tggtttggtc atgttcccat gtactcgatt cctcggaaca
     6001 tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct gtttcctggt tacattcgtg
     6061 gagagatcat agaaggtgcg tattcaggtg cagatctatt ctttttccct tcttatgaag
     6121 agacagaagg gatcgttgtt ttagaagctt tagcaagcaa acaaaacgta cttgttcgag
     6181 atattccagt gtataatggc tggttagaag atagcaagaa ctgctatatg gggaagacaa
     6241 acgaagaatt tatccgactg atccaacaga ttaccaatca agaactaccg agtacgacac
     6301 aagccggtta tcaaacagca aaagagcgaa gtatcaataa aatcggtgaa gaacttaaaa
     6361 atgtatacga aagtgtactg aatgaaaaag tctctagcaa attaaaaaaa gtaaatcagg
     6421 tcaaggacta gtttggaggc gaaaaacatg aaaatcggct tttttaccga tacctacttt
     6481 ccgcaagtca gcggagttgc tacatcgatc aaaacattaa aacaagaatt agaaagacat
     6541 gggcatgaag tctatatttt tacaacgacc gatcctaatg cggataagtt ggaaaaagat
     6601 gtgattcgaa tgccaagtgt accatttatt tctttcaagg atcgaagagt tgttgttaga
     6661 ggcatgtggt atgcttattt ggttgccaaa gaactagaac tagatcttat tcatacccat
     6721 acagaattcg gagcagggat acttgggaag atggtagcta agaagttgaa aatacctctg
     6781 atccatactt accatacgat gtatgaagac taccttcatt atatcggtaa aggaaaagtg
     6841 atcaggcaat cccatgtaaa atacttgtct agattatttg caaatcatac gacaggtgta
     6901 gtttgtccaa gtgaacgtgt tattgataca ttacgcagct atggtgtaat agcaccgctt
     6961 cgcgtgattc ctactggtat cgacattgaa aaattcaaac gttctgatat cactcagcag
     7021 gatatcagca aattaaggga gtcactaggt cttcaggaaa accatttgat gattctttct
     7081 ttaagcagga tttcttatga gaaaaatatc caagccttga ttaatgggtt tcctcaggtc
     7141 atctccgctt atcctaacgc cagattagtt atcgtaggaa aaggacctta tgtagaagag
     7201 ttagaggaat tgatctcaga actgcagtta gaagaatttg tgcagttcac aggagaagtg
     7261 gacaacaatg atgtagcact ttattataaa gcggcagatt attttgtcag tgcttctact
     7321 tcagaaacac aagggcttac ttatacagaa gcgatggcag ccggaacacc ttgtgtcgtg
     7381 gaaggaaatg cttatttgaa ccgattgttc gatcatcctt cattagggaa aacttttgaa
     7441 accgacgaag actttgcacc agcacttatt gattatattt ccagtgaagt tcctaaagat
     7501 ccaaaagttt tagaagctaa actttatgat atttcatcta cacatttcgg cgaagctatg
     7561 atcgaatttt atcaagacat ggttgtttat catgaagaag agatgcgaag aaaagaagaa
     7621 gaagactcga ttgaacgaat caaaataaaa ttaaattcat ttaaaagata aaattcaaac
     7681 attttttatg aaaaaatcct tgtttctagc gtatactagc atagaagcaa ggatttttga
     7741 tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga acaggcgtca tgggaagtgc
     7801 tgtcgcacgc catctactag aagcaggaca cgaagtagct gtctataatc ggacaaaagc
     7861 aaaagctgat cctttagtaa cagaaggagc tatctgggca gatacaccaa aagcggtagc
     7921 agaacaaagt aatattctct ttacaatggt aggttacccc aaagatgtag aagaaatcta
     7981 ctatggacag tcggggattt tttctgcaga tatctcagga catattttag ttgatttgac
     8041 tacaagtaca ccaagcctgg cagaaaaaat tgcaaaaaca gcaaaagaaa aaggcgcaga
     8101 tgcgttagat gcaccggtat ccggtggtga cttaggagca aagaatggta cactgacgat
     8161 catggtcggt ggagaagaag ccgtttatga tcaagtactg cctttattca aagaatttgg
     8221 tacgacattt acattgcatg gttctgctgg gaaaggtcag catacaaaaa tggctaatca
     8281 gatcatgatt gcaggtacga tgacaggaat gacggaaatg cttgtctatg cacaaaaaaa
     8341 cggacttgat ttaaagaaag tgattgagac gctttcaggt ggaagtgcag ctaactggtc
     8401 tttaagcaat tattctcctc gtattttaaa agaagattac acacctggtt tttttgtcaa
     8461 acattttatc aaagatttaa agattgcatt agaagaagca gaaaaaatgg atcttgtatt
     8521 gccagcaact acccaagcat tgaaactata tgaagagttg gcagataaag gttttgagaa
     8581 tgacggaacc caagctttga tcaaactttg gtggcctgaa ggaaaaatac cagaaaaaaa
     8641 atcataaaaa acgagttttt ttagagaaaa tcaatgcaag taggctatgc ttttgataca
     8701 atataacaga aataagaaaa ggaggacctc cgatgacaca ttcacaatta gtcgcaatca
     8761 taaaacgact ggaggctatg gtcgagtcag cagataatga actgcaagtt cgccgttttg
     8821 aaaaagaagg cgtagagaag tgtacagtaa cttatgataa agcgaccgat acatttgaat
     8881 taactgaaac agatactcat cagcaatacg aatttgataa tatcgatatc gtagcgatgg
     8941 aaatctatga cttaatccaa taaattttag gataattgac atttgaagtt gaagatgcct
     9001 aaaagaagcc gagaagcaac taatttagtt gcttttcagc ttcttttttg tttaacagtt
     9061 caatttcaaa ctgttctttt tctaattttt tgcggtaact gatcaagatt tgtacaggat
     9121 cgatttcttc ttttgacaat tgacgtttca aagataaatg agcttctttc ttttcataat
     9181 agaagtcagg agaaacgtca ccagaggtaa tcattgtttc tgcaagttga ccataacaaa
     9241 cgaggtagtg ctttttctga ctctcatctg ctgctaatgg catgctgaag gtgagcgatg
     9301 gtgttcttaa aaactgcttt tgttcctgga tccgtctaat aaaataagta actaattcga
     9361 tatctttttg aagcataccg gctttttcga aattcaactg atagatgaaa tgttgttgtc
     9421 tttttttcaa gtaagtcagt agctttttat ttcggccttg aaacagccct tggagttcct
     9481 tttttttctg ttcaaaagtc atttcctgta ggtcaggaag ctggactcgc aaagctaaca
     9541 aactaatttc agacaaccaa ggcatttgat aagtttcttc cattatacgc aaaatagacg
     9601 gcattttttt atagagacga aaaggcccgt atgttcgagc cgtaggagta tctgttaata
     9661 caaatccctt attggtaata tgtacataat tataattact gaaataattc atttgtcggt
     9721 tataaaaagg acggtaatgt tggatcaatt gacactcgag caataaagca tctaattccg
     9781 tgtctactac gacgaaatca aaatctgtta tatgatgaat catacgtaat actttttttg
     9841 aatgctgttt attctgatga aaatagctgc ttactctatt tttgagccgt tttgctttac
     9901 caacatagat gaccttacca tctcggtctt tcataagata gacacccggc agcatgggga
     9961 gagttttcat tttttctttt aacataggat ctccttttac gctttgaata aaaagactat
    10021 aacaaatttt gaaataatgg acaagtttta gggaattcta tagttctgtt cacagaaaat
    10081 actttga
//
LOCUS       DACCVU010000110         9912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCVU010000110 DACCVU010000000
VERSION     DACCVU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.111"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            168..1040
                     /locus_tag="IVW29_002347"
     CDS             168..1040
                     /locus_tag="IVW29_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7839812.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /locus_tag="IVW29_002348"
     CDS             1037..2995
                     /locus_tag="IVW29_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7839813.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3327..4178)
                     /locus_tag="IVW29_002349"
     CDS             complement(3327..4178)
                     /locus_tag="IVW29_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7839814.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4301..4768
                     /locus_tag="IVW29_002350"
     CDS             4301..4768
                     /locus_tag="IVW29_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7839815.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5031..5513)
                     /locus_tag="IVW29_002351"
     CDS             complement(5031..5513)
                     /locus_tag="IVW29_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7839816.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5613..6476)
                     /locus_tag="IVW29_002352"
     CDS             complement(5613..6476)
                     /locus_tag="IVW29_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7839817.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7074..7949
                     /locus_tag="IVW29_002353"
     CDS             7074..7949
                     /locus_tag="IVW29_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839818.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8094..8246)
                     /locus_tag="IVW29_002354"
                     /pseudo
     CDS             complement(8094..8246)
                     /locus_tag="IVW29_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8514..9845)
                     /locus_tag="IVW29_002355"
     CDS             complement(8514..9845)
                     /locus_tag="IVW29_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839819.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         3120 a         1971 c         1718 g         3103 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
     3241 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
     3301 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
     3361 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
     3421 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
     3481 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
     3541 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
     3601 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
     3661 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
     3721 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
     3781 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
     3841 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
     3901 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
     3961 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
     4021 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
     4081 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
     4141 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
     4201 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
     4261 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     4321 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     4381 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     4441 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     4501 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     4561 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     4621 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     4681 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     4741 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     4801 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     4861 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     4921 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     4981 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     5041 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     5101 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     5161 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     5221 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     5281 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     5341 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     5401 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     5461 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     5521 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     5581 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     5641 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     5701 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     5761 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     5821 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     5881 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     5941 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     6001 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     6061 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     6121 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     6181 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     6241 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     6301 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     6361 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     6421 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     6481 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     6541 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     6601 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     6661 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     6721 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     6781 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     6841 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     6901 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     6961 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     7021 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     7081 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     7141 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     7201 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     7261 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     7321 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     7381 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     7441 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     7501 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     7561 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     7621 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     7681 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     7741 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     7801 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     7861 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     7921 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     7981 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     8041 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     8101 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     8161 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     8221 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     8281 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     8341 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     8401 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     8461 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     8521 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     8581 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     8641 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     8701 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     8761 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     8821 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     8881 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     8941 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     9001 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     9061 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     9121 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     9181 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     9241 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     9301 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     9361 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     9421 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     9481 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     9541 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     9601 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     9661 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     9721 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     9781 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     9841 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     9901 atcacttatc aa
//
LOCUS       DACCVU010000111         9885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCVU010000111 DACCVU010000000
VERSION     DACCVU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.112"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..205)
                     /locus_tag="IVW29_002356"
     CDS             complement(<1..205)
                     /locus_tag="IVW29_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839820.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="IVW29_002357"
     CDS             complement(221..835)
                     /locus_tag="IVW29_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7839821.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="IVW29_002358"
     CDS             complement(982..1665)
                     /locus_tag="IVW29_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7839822.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="IVW29_002359"
     CDS             complement(1774..2232)
                     /locus_tag="IVW29_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7839823.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="IVW29_002360"
     CDS             complement(2316..4079)
                     /locus_tag="IVW29_002360"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7839824.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="IVW29_002361"
     CDS             complement(4300..5760)
                     /locus_tag="IVW29_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839825.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="IVW29_002362"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="IVW29_002362"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7839826.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="IVW29_002363"
     CDS             complement(6917..8344)
                     /locus_tag="IVW29_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7839827.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="IVW29_002364"
     CDS             complement(8375..9589)
                     /locus_tag="IVW29_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7839828.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2939 a         2051 c         1638 g         3257 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttc
//
LOCUS       DACCVU010000112         9811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCVU010000112 DACCVU010000000
VERSION     DACCVU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.113"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..2154)
                     /locus_tag="IVW29_002365"
     CDS             complement(34..2154)
                     /locus_tag="IVW29_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7839829.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="IVW29_002366"
     CDS             2384..3562
                     /locus_tag="IVW29_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7839830.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="IVW29_002367"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="IVW29_002367"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7839831.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="IVW29_002368"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="IVW29_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7839832.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="IVW29_002369"
     CDS             complement(4915..6168)
                     /locus_tag="IVW29_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7839833.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="IVW29_002370"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="IVW29_002370"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7839834.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="IVW29_002371"
     CDS             7746..9092
                     /locus_tag="IVW29_002371"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7839835.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="IVW29_002372"
     CDS             complement(9400..9786)
                     /locus_tag="IVW29_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7839836.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DACCVU010000113         9803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCVU010000113 DACCVU010000000
VERSION     DACCVU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.114"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(160..1227)
                     /locus_tag="IVW29_002373"
     CDS             complement(160..1227)
                     /locus_tag="IVW29_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7839837.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1247..1726)
                     /locus_tag="IVW29_002374"
     CDS             complement(1247..1726)
                     /locus_tag="IVW29_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP7839838.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1743..2204)
                     /locus_tag="IVW29_002375"
     CDS             complement(1743..2204)
                     /locus_tag="IVW29_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7839839.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2218..3468)
                     /locus_tag="IVW29_002376"
     CDS             complement(2218..3468)
                     /locus_tag="IVW29_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7839840.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3846..5297)
                     /locus_tag="IVW29_002377"
     CDS             complement(3846..5297)
                     /locus_tag="IVW29_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7839841.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5618..6382)
                     /locus_tag="IVW29_002378"
     CDS             complement(5618..6382)
                     /locus_tag="IVW29_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7839842.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6410..7108)
                     /locus_tag="IVW29_002379"
     CDS             complement(6410..7108)
                     /locus_tag="IVW29_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7839843.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7120..7899)
                     /locus_tag="IVW29_002380"
     CDS             complement(7120..7899)
                     /locus_tag="IVW29_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7839844.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7915..8859)
                     /locus_tag="IVW29_002381"
     CDS             complement(7915..8859)
                     /locus_tag="IVW29_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7839845.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8905..9684)
                     /locus_tag="IVW29_002382"
     CDS             complement(8905..9684)
                     /locus_tag="IVW29_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839846.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2823 a         2045 c         1492 g         3443 t
ORIGIN      
        1 cttgtactct tgctctatta caagcactaa aacactacta ttcatttgaa ttcttacacg
       61 gataaaaatt tacatcgtct atttcaatat tttttctctc tattttattc acttaataag
      121 ttcattacat aatcgaaaca ttttttattt tccttgtcat cactccttgc tccctttttg
      181 atacagattt aaaattctat caaagaaatt tccatcgtta aatttcgtta ttgccaaatc
      241 cttcgaacaa cgtgaaaatt ttacgtattt atctttatca ttgattttta gtattgcaga
      301 tgtcaacgct gatacgtcac caggttgaaa caaatatcca ttttctccat cacgtactgc
      361 tgaagaaata tctcctacat ctgtagcaat tacaggcatt ccataagaaa tcgcctctag
      421 aatagatata ggcaatccct cgttatagga cggtaaaatc atcacatttg atcttctata
      481 gcactcctcc tttttttctc ccacaaccca acctgtaaat tcaaaatacg attgcaaatt
      541 atattcttct atcaattctt tgagcgatat ttcttcatca cctgctccag caatctcaaa
      601 aataaacttt ttttctggat attgttctgc aatgttttta actgcttcta ccaaatcgtg
      661 tacacctttt cgctttatca aaactcccat aaacagaaat cggcacattt ttgaattcca
      721 ctttatggta tattctggaa ctacaataga attttctaca acaacagttt tacaatttgg
      781 ctcaatttct ttaattatct tcttccattt ttctccaagt acaatcgtgg aatcagactc
      841 tcttaacagc aagcgaatct gattttgttt ttttacagaa acactatcat accatttttt
      901 aaattccgat ccatgaagat ggatgattac aggaatattt aataatctaa accacttatt
      961 cagcacatat ttcctactaa aactccctct gtacgacatg tgcatatgaa taatattagg
     1021 tttactcaat aaagagatac ctataatttt agcaaatgca tacataaaat ataaagattt
     1081 aaatattgca tttccaccct tataactcgg tataaaagtc atctcgatat tctttttttc
     1141 ccaattaaaa ttgagcattt gtgtaattat acttgtaatt ccacctttta ccgagctgtc
     1201 attaccaatc ataagtactc tttgcataac gctttactcc ttatatttat attttttcta
     1261 ttaatacctc tatcgaatta ataatcgttt ccgtgttaga tacatacttg ttaaacatcc
     1321 gaactcttgt ttctttaacg gcatcttcta aattttctaa ttcataacaa ggatagataa
     1381 tattcatctc atcaaattgt ctaattaact gcagttggtg atcatcgaca tgctcaccat
     1441 acttagccaa tcgaggaaca gcaatgaccc ttttaccttt ttttacggcc ccaattatag
     1501 cacctgtacc tccatgagta atcaccattt ctgctttttg catagtttca gtaaactcat
     1561 ccctatttag aaatgcttta taggaataat ttagtggttt ataatcacta taaccactct
     1621 gtgcaaatgt actttcgatt attactcctt tttcaattaa atcatcaata gcttttaaca
     1681 atctatcaaa ttgaaatttt tgtgaaccta atgttataaa tatcattttt ttctcctatt
     1741 atttaatata tcccacccaa ataaatagca ttaggataaa attgtagcat agtttcccac
     1801 tgaacataaa attgatctgc aaaatgatac atcaattttc ctgtctgagt agggctggtt
     1861 acttttgcaa acgattcaat ataaatcagt ttttttccca tcatttttgt tattaagcag
     1921 atagggatca tagctaaaac acctgtacaa atcacgagat ccggtttttc ctttatatat
     1981 ataataattg atttgaataa attaataagt atagtacaca aaaaaaactt ttcttttcta
     2041 tttacttgtc tcatgtagta cgttttttcg ccttttgact cagctgaata ttctgtctgt
     2101 tctgttataa caaaactctc atacttctcc atcagtggtt ttaacatcat taactgttca
     2161 taatgccccc cagaggaagc cgcaaaacat atctttttgt tcatattaac ctctctatca
     2221 gtcacgctga aaaatatctc tagtgtaaac tttttcttta atatcatcca agcaggtatc
     2281 atatcgattg gcaataatag aatcacacat cgccttaaac tgctctaaat cgttgataac
     2341 cttacttccg aaaaaagtct ctccatcttt cagtgcaggc tcataaatca ccacagtcgc
     2401 acctttagct tttacgcgct tcataactcc ctggattgaa ctctgacgaa aattatcact
     2461 gttgctcttc atggtcagcc ggtatatacc gattaaaact ggattttccc tgttgacatc
     2521 atactgatta tattcagcgt agttgtaata gcccgccatc tgaagaacac gatctgcgat
     2581 gaaatcctta cgggtacgat tagattcaac gatagcctca atcaaatttc caggaacatc
     2641 tgcatagttt gccaacaact gcttggtatc ttttggcaag caataacctc catatccaaa
     2701 ggatggattg ttgtagtgag ttccaatacg aggatcgaga caaacaccat tgattatctg
     2761 ctgagtattc agccccttca tttcagcata agtgtctaat tcattgaaat agctgactcg
     2821 cagtgctaaa taggtatttg caaacagctt tactgcttcg gcttcagtga atcccataaa
     2881 aagagtatcc acgttctcct taattgcact tttctgcaga agtgttgcaa aaacatgagc
     2941 agcatttaac aagcgctcat cagtcatatc agtaccaaca ataatacgag atggatacag
     3001 attatcatat aaagccttgc tctcgcgcag aaactctgga ctaaaaataa tgttcttact
     3061 ccccgttgtc tcacggactg attcagtgta gccaacagga attgtagact tgatgaccat
     3121 gatggtatca ggattcacgt ccatcacaac ctcaataact gactcaacag cagaagtatc
     3181 gaaatgctga gtacgactgt catagttagt cggtgtagca ataacaacga aatctgcgga
     3241 tttataagcg ctccttgcat ccaaagtagc agttaagtca agttcttttt ctgtaagata
     3301 cttttcaata tattcatcct gaattggaga cttccgttta ttaatcagtt caatcttttc
     3361 agggataatg tctaccgcgg ttacctggtg atgctgcgcc aatagtgtgg cgatactcaa
     3421 accaacataa ccggtgccgg ctactgcgat gttgtacttt ttcttcacaa tatctcctct
     3481 ttcctctctt aagtatggag aatgttactc ctacggctat tcatcatgtc aaaataacac
     3541 agcttagaat gttgataaaa taattttcca gaatgtgata aaatctcttt catttataaa
     3601 ccacacatct aattttatct attctccaca agacatgtag agtattcttt aataacaaga
     3661 tacgacctta ctaaatactt caaaaaatta tcatatttaa ttcataaata taaatcttca
     3721 ttttaaatgt atgcccaaaa atttatctgt aatgcgtcat caaaatctag attccaaatc
     3781 tttatcaatt tttatccact cattctacca ctttagtaat attttctatt tataacactt
     3841 tcgtcctaca ttgatccccc cctctgaagt acagttttta ctgtcttaaa taaaatttta
     3901 aaatctaacc ccatactcca attttttata tattccgtgt caagttttgc tacttcttca
     3961 aaatcagtaa tatcacttct accacttacc tgccacattc cagtaattcc tggcttaata
     4021 gcaattcttg cacgatgatg ataatcatat tcttcatact ctgatttaag gcatggtcga
     4081 aatcctataa ggctcatctt tcctgttaga acgttgaaaa attgagggaa ttcatctaaa
     4141 gacgtggatc taataaattg accgattcct gtcttttttg ttccatctgg aagtatttta
     4201 tttccgataa ttcttggatc aaactccatc ttaaacataa gattattgct catcttattt
     4261 tctttcatta attctacttt tctttcttct gcatccatat acatacttct aaacttatac
     4321 atcttgaacg gtttaccatt ctgacctata cgttcttgtg caaagaaaat tgggcctggt
     4381 gaattaatat aaataagtgg agcaataaag ataaaaataa ttcccgttgc aatacatcca
     4441 acaatacctg caagaatatc aattgttcgt tttataaatg cctgttgagt tgtcataaca
     4501 ttgatacttg ttgtaagtac tgtatattct cctatacgac caatagattg ttttcccaaa
     4561 gcactatctg atacttttgc aagattcaaa tgtacagtaa ggcccatttc agagcatcgt
     4621 tcaataaatt ctttcataca cgaagtactt ttattcaaaa taataaagac ttcatctacc
     4681 catccctgac ataagtaatc cgctgcattt ttggaatcag aaacaactgt cactccctca
     4741 acatcttcac caatcatgct tttatctatg ataatgacac catttatttt atacatttta
     4801 tagttatgct ctttcacatt acgaataact acttcagcaa tactactaga tgtaactata
     4861 ataagtgatt tgttcccctc atttttcatt ttatgtttca agtaatgttt ccataagagg
     4921 cgagttatat atgtaattac aacgtataaa cctcccatca agaaaagaac gactctagaa
     4981 taatcatttc caccctgaac actaatgaga tacaaacttc ctaacagcac caacataatc
     5041 gcttgcttta cagaagaagc aaattctata tagtaccctc tttttaatac acctctatat
     5101 gcttcaaaca tgaaaataac taaaagatct gccagttcaa tgaagactgc catgttttga
     5161 tacattggaa tcacataagg attccaatct acctggcgta agacatacga tagataaaaa
     5221 gcaagctgga tacacaataa atctaaaata ataaaatcaa tatgtttaac ccatcccctt
     5281 gaacttttct tgtacattcc tttttactcc cctcttttta ataaaataaa ttgtattact
     5341 tgttttctct cctaaatatc tttctgaagg attattgact cgacaaattg gcttgtggat
     5401 attcttatat atagaacatc cgtaagccaa cttaaaactc cttaacaaaa tttttttact
     5461 catattgtag atcaaaacaa gtatccaaga aatcatattt tatttctttt ttctgcttac
     5521 tcaacaacag ctaaaagaaa aatcatcata aacaaattca actaagccaa cgatcccctt
     5581 gtgaaatcaa gttttttctt gatttacgaa taaagagcta aaataaacca aacttcttct
     5641 tttttacctc ttcatagact ggataactga tttcatcccc gttgatcaga tcctttgcga
     5701 cttgtttcat ctgtactact ttttccttac caaagtcctt tgcaatctgc tcatagcact
     5761 cttttaagta aaaggtgcgc ttagagaccc catgggcatc agaagcagcc atctgcacga
     5821 gattatgttt gaccatcgtt ttggctgttt tctggatgtc tttgccaaat accccaacat
     5881 aacttggcgc agtgagttgg gccaaacatc ccatatctag gaacggaagc aaatgatttg
     5941 ggtcttcgcg aaaatgcacg ttgcgttctg gatggacgat tactggtgtc tttcctagtt
     6001 ctaacaactc aaaaaaaagc cgttctgcat ataacggcac ttccagtgtc ggaaattcga
     6061 tcagaagata tgtatcatct aaatctgtaa ataatatttc atctcggtgg atgtcttcga
     6121 tgagcgttcc ggtaatacgt acttcttgtc cttccaataa cgtcaaaggc aactgtcttt
     6181 tctctaattc ggcttgtaac gaagcgacta gagaaatgac ttgtgatttt tcattgctgt
     6241 attttccatt attgtggtgt ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt
     6301 tttcagccat tgcaatactt gcttctaagt tttctgcccc gtcatcgatt cctggtagga
     6361 tatggcaatg tagatcgatc atttctcttg tctcctctct tcttattcct taatttccgt
     6421 agtaataacc agcatctttg ctatgttctg ctccgttgta cacaacacct aagactcgtg
     6481 cttgtaccat ctccagcagt tcttttgctt tagtcaagga ttctttacgt gacatatttt
     6541 cacgtacgac caataaagtg ccatccgttt ttaaagacat gatctgtgca tctgttacgg
     6601 ctactactgg tggcatatcg aatatcacca cgtcaaataa ctgtcttgct tcggatagga
     6661 tctgatccat tcttggcgag cttaacaatt ctgatgggtt gggtggctta ggtccactag
     6721 gcaaaatcga caagttctcg actggcgttc gttggatcac atctgctaca cttcctgatg
     6781 aactaagtgc tgtacttaat ccactggcat tgttcagctg gaatgtctta tagacgacgg
     6841 gtttacgtaa atctgcatcg actagtagaa ctcgttgacc tgatttagca aaaacgactg
     6901 caatgtttgc tgcagtggtg gatttccctt ctccaggacc tgatgaagtc acgacaatcg
     6961 ttttgatttg ctgaccagct gcggaggcaa actggatatt cgtccggatc gtacgatatt
     7021 gttcggcgat tggtgaagat gggtttgtta atgtgatcaa actgactgca tgagtttgtg
     7081 ctgctttttg tttctgtgtt cgtgccattt ttcttcctcc tagactcttg agcggcttct
     7141 gcgtgatggg gctgttttat cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt
     7201 acggatacca gcgttcaatt ctttcgctga catttctgga actgttccta agattgggaa
     7261 tcctaatgta tctgtcacat atttttcatc tttcactgtc cgatcgagta attccaataa
     7321 gaatgccaga cctacaccta ccatcattcc taacattaag ccagtcatta aattcaattt
     7381 attatttggc gaaacaggtg tcgtattggc aactgcacct gaaatgatcg agattttatc
     7441 aacattcatc acatcttttg cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg
     7501 ttgagcagcc actgcattcg tacttattgc ctgaatcgag aacatttgtg aattttctgc
     7561 ctgattaact gagatggcat ctttgatttg ttcagcagtc attttcacat tgtcttctgt
     7621 ttctaaacga tctttgactt cattgagcac taaatcactg acgatcatat ctttatacgt
     7681 attgatcatt tgcaggttcg tattgacatc attgacattt gcagtgtctg attgtggcag
     7741 tgtcacgatc aattgtgctt gtgaactgta ttttggtgtc aagatgaaaa atgttacgat
     7801 tcctgagatg gccaagccag caaacattga gatgatgatt attaccaagt gtttgcgtaa
     7861 tgtttcaaaa atttctttca agctgattgt ctcttccatt ttttctccac tttcctattt
     7921 attctctaga ttcatttgat cttttaattc ttttttcaca cgatcaagtt cttgatcact
     7981 gactttttga taggagatat tatcttgcat gaagccttct ccttgcagct gatcttgctt
     8041 aatcgtacca aatgcctccc gataatctaa agcaattttt ttcatttcat caaagttcat
     8101 gtctgttttc atattcgact ctagagcatt caaaacggat tgatagttgc tgacaccgtt
     8161 tagactcaat acttttttcg ctacgccttc tacgatcttt ctttggcgcc cttgtctacc
     8221 gtaatcacca tttgggtctt cataacgcat acgagaataa gataatgcct gatccccgtt
     8281 caatgtaatt ttaccgattg gaaagtcata gccatcttgt gagaaagtga ggtcattatt
     8341 gacttcgata ccaccaactg catcaacgag ttcctttagt cctttcatat tgatagagac
     8401 atagtgatcg attgggatgt ctacatattt ttgtacggta tccatcgcca tggcagctcc
     8461 gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca cgacctacga tgtcgacata
     8521 cgtatctcga ggaatgctga cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac
     8581 catcatcgta tctgatcttc cttgatcgac ccgacctaaa tctccagtat caatccccat
     8641 caatagaaca gagaaagatt cttgtttgtc cagatcaact ggtttcgttc gttctccttg
     8701 ggttcgttcc actgattcgt aagttttctc aatagaatgt gagagatcca gatataattt
     8761 tgctcctact cctacaattg ctaatactaa tacgataaga actgctaaaa taatcaaaaa
     8821 tatttttttc cctcttgatt ttcttcttcg tcttgccata gtttcctccg tttgctaacc
     8881 ttgcttgaat caacgtttta actgtcaata gcttatattg ctatagccca tttgctgagc
     8941 aacagtttgt gcttttgttg cgtatgcggc tcttattttt tcattccgga tctgctctat
     9001 ttgactgagc gccgtcaaat attgatcgta agtagcggta gctgttactt gaccgtcttt
     9061 cagcatcgta tcgaatagct gatctagttg cgtgacttgt gcaatttggg tatcagcgaa
     9121 tcctagatat tgcataacga ctgttttcca agcactatcc ttaaaaaagc tcatattttc
     9181 acgtacatgg gcgacatctg cacttgccaa cttttctttg atgatgacat catccacagc
     9241 ttgctgccag tttgatacgt ttttagtaaa taatttattg acagccgtct ggatcttcag
     9301 ttttgattct gcatcctcta actgcgttaa gactgctttc ttttcttctt ggatcgcagc
     9361 agcttcttta ggtaattctg attccttgat gtcataatca tctgccgaaa gtttgataga
     9421 agatagttta tgtgacaaat ttgaaactgt ttcttctgtt atttcaggat ttaggaaaat
     9481 tttttctttt gtactgtaca gcttatctac ttcttttgat aaagaggtca cggtgtcttt
     9541 ttgattggtg atggcagatt ctgcgtaatt gatccgttga tgatattgcc gctgattatt
     9601 gagatggtat ccaaacaagc aaatcacaat agcgcctgtg cctaccgcca cagtagccaa
     9661 taacttgacg aacggctttt ccatagattc actcctaaat attgatgata gataagtaaa
     9721 gatgtgattc attcacatca gaatgagcgc tttttatttt aaaagcataa ttaaactaat
     9781 atattcatag aaatatttat aaa
//
LOCUS       DACCVU010000114         8992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCVU010000114 DACCVU010000000
VERSION     DACCVU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.115"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            705..1037
                     /locus_tag="IVW29_002383"
     CDS             705..1037
                     /locus_tag="IVW29_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7839847.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1628..1957
                     /locus_tag="IVW29_002384"
     CDS             1628..1957
                     /locus_tag="IVW29_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839848.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTNDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1944..2285
                     /locus_tag="IVW29_002385"
     CDS             1944..2285
                     /locus_tag="IVW29_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7839849.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2399..2638
                     /locus_tag="IVW29_002386"
     CDS             2399..2638
                     /locus_tag="IVW29_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839850.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2705..2944
                     /locus_tag="IVW29_002387"
     CDS             2705..2944
                     /locus_tag="IVW29_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839851.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            3071..3760
                     /locus_tag="IVW29_002388"
     CDS             3071..3760
                     /locus_tag="IVW29_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7839852.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            3766..4932
                     /locus_tag="IVW29_002389"
     CDS             3766..4932
                     /locus_tag="IVW29_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7839853.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            5014..5685
                     /locus_tag="IVW29_002390"
     CDS             5014..5685
                     /locus_tag="IVW29_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7839854.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5738..7714
                     /locus_tag="IVW29_002391"
     CDS             5738..7714
                     /locus_tag="IVW29_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7839855.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYQAHAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            7727..8479
                     /locus_tag="IVW29_002392"
     CDS             7727..8479
                     /locus_tag="IVW29_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7839856.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            8495..>8992
                     /locus_tag="IVW29_002393"
     CDS             8495..>8992
                     /locus_tag="IVW29_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839857.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFS"
BASE COUNT         3169 a         1337 c         1856 g         2630 t
ORIGIN      
        1 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
       61 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgtttt tttgagttat
      121 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
      181 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
      241 aaatttaatt gtctgatatg cttgtgatga attgattttc gttctgcact tgataattga
      301 aaatacaaaa aaaagaaagg cccctgtaag gctcgggaga ctggtaatct ccgtcagtgt
      361 cctacaggaa agcattaaga tgacggctta atgcgtgccc ttcagtgtta cggtattacc
      421 cgcaacggac tcagtataac agatattcaa aagtgatacc actttttttg gctctattaa
      481 ggttaaaaat acgtgatatt gtaatgaatc tatgcctaag cataatgtta taaaaatgtc
      541 cgtgtggctc tttgccggat gacgaatgct gaaattagag ggaacgcatg tttggatgcg
      601 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
      661 aaaaacttat taaaaatatt taaagaaagt gagaggatta ttttatgtca tatacaggaa
      721 ttttgagtct cgaagatatt tgtcattacg gtaaacggtg taccgctaca gagaaaatca
      781 cgaagaaact atcaactggg caaagcaaag ctgttgtgca gtgtaagaaa tatataatcc
      841 agaaagacaa agtttcagaa gaaatgattt attacactgg gaaacgcaag caaataattt
      901 taaaagatcc tatcccttta aaagagctat atccaacaat caaacatgtt tatgaccaaa
      961 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     1021 ggcgtttaat cagctgaatt aatcactctc ataacttgta ttttatcgtt tttcgcttgc
     1081 ttgaaaatat tgtcgagact tctaaaggaa cttagaatca ttagaaaaat aaggagtaga
     1141 cttgatctat caacaattcc tttcgggggg tgacggggta ggtttttacg attggtaacg
     1201 taaaaaattg gtcaatcgag taaatgccct tgtttggata gtggtgaaat gaatttgtgt
     1261 aaataatatg cacagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgc
     1321 ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact catggaataa
     1381 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     1441 atgtagatac ttgcgaagac gttgatcttt ggatcaatcg agcatttacc gttttctact
     1501 agggtttctc gtttcggacg ccataaacga gtcgatttat tttatatatt tttgagtgaa
     1561 cgaaaaacga taaagatatt cgttatgaag taaaactaat ttagtagata gcaaaggtgg
     1621 tagaggaatg attcaaaaat ttgcgaaaat gagtaaaact gcatggattg aaaagcttga
     1681 aaatgacttg agtgtcatgc gattagctcc aaagaaattc aaacgagaga ttgcagaaaa
     1741 agaagcaatt ttgtcggtat tgaatgcggt agacgataaa tgtccagtaa aaattagttt
     1801 ttcagatata gcaacaatta tcgaaggtat ttattatggt gaagttgatg aagagatatc
     1861 ttctacaaat gatttattag attttttgga taaaaacaaa gataccttta tagtactaac
     1921 tgattatgag gaggttacaa caaatgaaaa attatgatcc gaatatacga ttgggcacac
     1981 atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag
     2041 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
     2101 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
     2161 ggtttaaaat gacgcttacg gatgataatg gagacgaatt atctgttgaa gatacttggt
     2221 catatttgag tgattatatt gtcagcgttg aaatcattga atttgtagcg gataaagaag
     2281 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgacgaataa
     2341 taaaaaggta gtattacctt aggcattgag taaggaattt atttaggagg aaaattagat
     2401 ggcgcgacca gttttagagg cgatggatat ggatactttg acaaaaggaa ggtatgtaca
     2461 gctgttagct cgtggttatt cacctaaaga tgtattcaaa tccctgtcta aaggaacaga
     2521 tatggaaaaa gaaagattac gaaaagaatt cgattattgg agaacccata atggaatcaa
     2581 ggatttgaaa ccagcacggc cagtaattaa acgaaagaaa aagataaccc aagactgaag
     2641 tgtagagcgt atgtgatcat aggctttttt atttttgcaa aaaacaagaa aggtaggaaa
     2701 actcatgaat tatgaagaaa tcgaaaacag aaaaaaagtg tcgaaagaaa tggaagaaaa
     2761 gttgctaaaa atgatgaaac aaaaacacct taaaagatta tctgtcatgc aatatatcaa
     2821 tgatatgaag ataacaggaa aagagaaagc ttgtttgtta ggttcaatga aaaactttga
     2881 acagttgagg cgaacttatg taaaaacagg ttccaattgc caactattat tagaagtttc
     2941 ttgacgatta tgatatgaaa atgatcaatt atgatgtggc attgttattg aataaaaaga
     3001 atagtaaaat ttaacatggt ttatcttggt tagatgtagg taaattaatt catatataga
     3061 aggtagatag atgaataaaa acaataaaac tttatttaat ccttacgtgg gcgtaatcat
     3121 tgtttttctt ctttttagtt attttgctta tcaaggttat caaaaggaca aaatcgctga
     3181 aaaagtagat ggtgtaacaa aaacaatcca tattcaggaa caggctaaaa cctatcaaga
     3241 taatttaacc agtttaacgg atatttctaa gagacaactg attactaatc aaaaacaggc
     3301 attagaaaag gatctcaaag gtgaaattgc tggaaaactt gttttttctt ctacaggatt
     3361 tattctccct gtttttcaag gagcgaatag cgacacgctt agtttaggag tagcttctac
     3421 ttattatcta gatactgaaa tgggaaatgg aaattatatt cttgctggtt ataacgtaga
     3481 tacaccaagc gttttattat ccgatatgac tgatttatct gaaggagaag aaattgatct
     3541 aatcgatgct cattacaagt atcagtatga agtctccaaa agaggaaaaa tttctgatta
     3601 cagccaggta gttcaatcta aggaaaacaa ttccttttta tccttgccaa aagcaggtaa
     3661 aaaaccacta ctcacactgt tagcctttca agcaaataat atgaaaaagt gcgatgtgat
     3721 ccagtgtgaa ttagtcaatg tagaaaaaat caataaataa gggaagtgag tcacattcaa
     3781 gaacttgtag aaaaattgat caataaaatg agtgccattt ttctgttact gttggtatcc
     3841 attctatatt taatatggac ggtggggtta aatcctccta cgatattatt tatttatgat
     3901 aatgctgcac agttagtgac gtttttcgta ggctgtttgt ttcttttgaa tctgtacaag
     3961 gtaaagagaa acgatctatt cttcattgca gcaggcattc ttttttacct tttcttttct
     4021 ttttttagaa gctaccgagg agatactttc tatggtgatc tgatcgttcc gacaattatt
     4081 gctattattt tgattgtaaa atggacgaaa tttactgtaa ttgatcgtag cttgtatctt
     4141 gtagtattct atgtcttttt actcgtaacg gtttatcgag tatttactga gataccagta
     4201 ccagaaggac aaagtatttg gaatccatcg aacaaattat cagatatttg gatcaataca
     4261 aatacgatag gtagttcttt aatgacatta gcccttctta tcacaggatt tgctagttca
     4321 tttggaaaat ggtacatgcg attgctatca ttaccagcgt tgattcttgc tggggcaacg
     4381 atttgggttt gccaatcgaa agcagcatta tatgcgcttg ttatcttcat tatcttagaa
     4441 attttaccta agaaggtttt tcgaaataca cacttgtcct tttggatgta tgcagcagtt
     4501 tcggttctag ctttaccgat ttcttatttt gctgcaatgt cagacaaagt tcacttattt
     4561 acaggacgag aagaaatttg gcgcaaattc tatgaaacat taggcgaaaa aacaggacaa
     4621 atatttgtag ggatgaaacc tttctttttc catagaggaa atcagattct aggtaatcat
     4681 aatacgtatc acgcactcgt caatcactat ggattgattg gttttgttct atcgattatt
     4741 cttttgctta gtttcttatg gttgctcgta agaaaacgtg agctgacaaa tggacaacta
     4801 acattcttgt gggctttttt agcgatcatg tgtcaatcgt tcatggaaga aacattaacg
     4861 acaatcagtt gggtgccaat tgtattctta atgttagcaa tggctacaca ccaatataaa
     4921 accaccagat aaatgaaata gaatagtaat aagagaaaac actttttcgt tgtgaaaaag
     4981 tgttttttgt tttgtctgaa aggaggcaga tggatgaaaa aatggttatt tggtttttta
     5041 ggagtcgctt taatcgtcgt ttgcagtgtg tttggctatg ttagctacca aaaacatgaa
     5101 ggagaggtgt ttaagcaaaa tatagaaaaa aagatgcccg tagatcaaat caatgcacac
     5161 gcaaagagtt ataaggagga tgcaacgaac gtcaataatg atatgtcttt ggggcaaatg
     5221 ttgtccatac aaaaagaagc aatcgaaatg ggcgtcaata aacaagtatt tgcacaaatt
     5281 cagattcctg cacttggttt agcattgcca atttttaaag gggcaaatca atacactttg
     5341 agtcttggag cggctactta tttttatgaa gatgccgaaa tgggtaaagg aaactatgtc
     5401 ctagcaggtc ataacatgga aatgccaggt gttttatttt ctgatattca aaagttgtca
     5461 ttaggggagg tgatggactt agttagtaac gatggcgtct atcgttacaa ggtaacgcgt
     5521 aaatttatcg tacctgagta ctttaaatta atcgatggcg ttcctgaaga gaactctttc
     5581 ttgtctttac caaagaaagg agagaaaccc ttattaacgt tgtttacttg tgtttatacg
     5641 tcacaaggaa aagaaagata cgtggtacag ggagagctac aataacaaga atttaatcaa
     5701 cgcgagtcgt tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc ggtatgtaaa
     5761 aaaagctgga atgattgttg gaatgttagg aatcattgca ggctgctttc aggggtatca
     5821 agcacatgca gaagatgtca cgcaaaaaac gccaccagaa agggtcaata tcacggttca
     5881 taaattaatg tacgatcaaa gtacgcaatt gaacgttgat attgacggaa tcaaaaatga
     5941 tggttatacg cacgatcaat atcctacagg tgtaacgaaa tacaacaagg cagattatgg
     6001 tgatgttgag tttacgttag gaaatattac ggatcaagta ttaccgactg aagattctga
     6061 tctaaccaac gcgaaagtgg acgaaatcgt taaagatgta gaagacaagg gatctaacag
     6121 tgagtacgtt aagaatgcaa ccaataaaat tacttcagct gtagatgaaa atggtgagat
     6181 cacttttgct gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg tggtttatga
     6241 aagtaaatcc gctgctggat tagtcacaca aaaagcaaaa ccaatggttg tgattgcgcc
     6301 aatgacagac aacacaggat caggattttt aaaagatatt catttgtatc caaagaacat
     6361 tgtttctaag ttgagttttg aattaacgaa atttggtgac gatggcacag ctcaaagcaa
     6421 acaaacacct ttaaaagggg caaaatttga gttatacaag ggcgaacctg gaaaaggaac
     6481 aaaattagga gagcttgtat cggatgatca aggaaagctg actgcaactg atttgacgtt
     6541 aggaaaatac tactttgtgg aagttccttc agaagttgta gttggatcag atcaagaacc
     6601 aacagcagac caatatttgt taggcgcaga cgctcgcaac gatgcgcaca ataagttaac
     6661 ctttgaaatt acgaatgacg gcgtgacaag tgatttaaaa gcttcctacg tgaactataa
     6721 agcaccagtg atcgataaaa cagtgacaaa tggaacagga gaagaacatt ctttccaaat
     6781 tggtgacgca gtgaattatc aaggtacaat tcatatccct acagatatcg caggtggaac
     6841 agatgggatt acagtaaatg gtgtaaaaag tgaaacttct ccttacagtg tatttaaatg
     6901 gggcgacaca gcaggtaaag gactttctta tgttgctgaa aaagcagata ttaaagtaac
     6961 aaataatgat ggttcagtcg tattaaaaga aaatactgac tataaaatcg aaaattctga
     7021 tagtggtttt gtgatcgatt ttatcgtaaa taatggacaa gtaagtgata cagttgcgaa
     7081 tttacatggt caagatctaa aaatgactta taacatgttt gtgaatgata gtgctgaaat
     7141 cgcaaatcca ttaacaaata gcgtggattt cgtttataac aataatccat ttaatcagga
     7201 agaacaccac gaaaaaacga aagctgacgt tgtgacttat ggcgcaaaat tcttaaaagt
     7261 cgatagcggc ctatttggaa caggtatcaa agcaacaccg cttgaaggcg cagaatttgc
     7321 agctaagaat gcagaaggta agtactacgg tggacttact gatacagata aagatggtgt
     7381 caaagaagca gtttgggtag acgatgtagc aaatgcagca atcctaaaat cagataaaga
     7441 gggtcatttt gaaatcactg gtttaactga aggagattac tcactggaag aaacaaaagc
     7501 tcctgaaaac tatcaaaaat taacaaaaga gatctctttc aaagtagaca aagattcata
     7561 caaagaagaa aaccgaatca cgatcaaaaa taatcaaaaa gcttccgtac caatgactgg
     7621 tagcaacggt ttccaatctt acgtattgat ctcttgcttg ttgcttgggg caggcgcaat
     7681 ttctgcagtt gtttacttta agaagaaagc ctagaaggat cagctaatga ttatcagaca
     7741 ccctaagaaa aagcgaatca tgggtaaatg gatcattgct ttctggttac tttcagctgt
     7801 tggtgtctta cttttgatgc ctgctgaagc gagcgtggcg aaataccaac aaaatcagca
     7861 gattgccgca atcgatcgaa caggaacggc agcagaaacg gattcttctt tggatgttgc
     7921 taagattgag ttaggcgatc ctgttggtat tcttacaatt ccaagtattt ctttaaaact
     7981 accaatttat gacggaactt ccgacaaaat tttagagaat ggtgtaggga ttacggaagg
     8041 aactggcgat attacaggag gaaacggtaa aaatccttta atcgcagggc acagtggact
     8101 ttataaagat aatcttttcg atgatttgcc ttcagttaaa aagggagaaa agttttatat
     8161 caaagttgat ggtgagcagc acgcttatca aatcgatcgg attgaggaag tgcaaaaaga
     8221 cgaactacaa agaaactttg tcacttactt agaaccaaat cccaatgaag atcgagtaac
     8281 gctaatgaca tgtacgccta aggggatcaa cacacaccgt ttcctagtgt atgggaagcg
     8341 agtaaccttt acaaaatcag aattaaaaga tgaagaaaat aagaaacaaa aattatcttg
     8401 gaaatggctt ttaggaagta ctgtttttct ttcagtcatg atcattggtt cgctatttgt
     8461 ttataaaaag aaaaaataaa ggagggtaac caacatgaga aatagaaaat ggctatttgt
     8521 ttgtgcagcc gctctttttt ctttcactgc ctacacacag ttgacgtttg cagacagaca
     8581 agaaagcacg gatagcacag aacaaacaat ccaaacgtca gatagcagca cgattcaaag
     8641 cagcgatgaa accgtcgatt cgtcaacagt tgattcttca gaagaaccta aaattgctga
     8701 agacgatcaa atagtcaaac agaccattca cgttcaaaaa attatttctt ccgaacaatt
     8761 agacaaccag gggcaaaagc ttgaaaaaca agaaggagta aatggcgcga agttttctgt
     8821 ttatgatgta tcggacattt tacaaaagat ggatgttaag gatctcacta ctgaccaaat
     8881 tgaatcccaa ttaaaagatc gagtgaaaaa actatccagt gatcaactga agctaatttc
     8941 taatggagaa acaaagacaa ttgatcaaca aacaggtgtc tttgaattct cg
//
LOCUS       DACCVU010000115         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCVU010000115 DACCVU010000000
VERSION     DACCVU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.116"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..260
                     /locus_tag="IVW29_002394"
     CDS             51..260
                     /locus_tag="IVW29_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839858.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IVW29_002395"
                     /pseudo
     CDS             467..667
                     /locus_tag="IVW29_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IVW29_002396"
     CDS             complement(1379..1984)
                     /locus_tag="IVW29_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7839859.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IVW29_002397"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IVW29_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IVW29_002398"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IVW29_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IVW29_002399"
     CDS             3019..3990
                     /locus_tag="IVW29_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7839860.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IVW29_002400"
     CDS             4130..5443
                     /locus_tag="IVW29_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7839861.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IVW29_002401"
     CDS             5486..6352
                     /locus_tag="IVW29_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7839862.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IVW29_002402"
     CDS             6349..7197
                     /locus_tag="IVW29_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7839863.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IVW29_002403"
     CDS             7211..8683
                     /locus_tag="IVW29_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7839864.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IVW29_002404"
     CDS             8700..>8942
                     /locus_tag="IVW29_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7839865.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACCVU010000116         8901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCVU010000116 DACCVU010000000
VERSION     DACCVU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.117"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1153..1551)
                     /locus_tag="IVW29_002405"
     CDS             complement(1153..1551)
                     /locus_tag="IVW29_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839866.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1767..2822
                     /locus_tag="IVW29_002406"
     CDS             1767..2822
                     /locus_tag="IVW29_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7839867.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2842..3195
                     /locus_tag="IVW29_002407"
     CDS             2842..3195
                     /locus_tag="IVW29_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839868.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3377..3673)
                     /locus_tag="IVW29_002408"
     CDS             complement(3377..3673)
                     /locus_tag="IVW29_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839869.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3677..4495)
                     /locus_tag="IVW29_002409"
     CDS             complement(3677..4495)
                     /locus_tag="IVW29_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7839870.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4721..4942
                     /locus_tag="IVW29_002410"
     CDS             4721..4942
                     /locus_tag="IVW29_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7839871.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4944..5210
                     /locus_tag="IVW29_002411"
     CDS             4944..5210
                     /locus_tag="IVW29_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7839872.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5323..5862
                     /locus_tag="IVW29_002412"
     CDS             5323..5862
                     /locus_tag="IVW29_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839873.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="IVW29_002413"
     CDS             6356..6640
                     /locus_tag="IVW29_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839874.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6914..7168
                     /locus_tag="IVW29_002414"
     CDS             6914..7168
                     /locus_tag="IVW29_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839875.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="IVW29_002415"
     CDS             7250..7435
                     /locus_tag="IVW29_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7839876.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="IVW29_002416"
     CDS             7502..7897
                     /locus_tag="IVW29_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7839877.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            7887..8264
                     /locus_tag="IVW29_002417"
     CDS             7887..8264
                     /locus_tag="IVW29_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839878.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8660
                     /locus_tag="IVW29_002418"
     CDS             8265..8660
                     /locus_tag="IVW29_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839879.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         3120 a         1495 c         1391 g         2895 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaaatca aaatttaaat gttttaatat tacaaataaa atcatgttat
      541 tgtaaaatgg tataaaaaag ctaaatattt tatttttgtt attttattag caggaaaatt
      601 taaatagttt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaagta tcaacataaa catatacata tacacgttta tgttgattca cttaatttgt
      841 cctttaaaaa gcacttattt taatataatg ttattttata atcttattac aagctgattg
      901 ttaggcgttg tcctttcatt acatttatta acggttattt tattgtttaa tttattaatt
      961 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
     1021 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
     1081 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
     1141 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
     1201 agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga
     1261 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
     1321 tctaataccg tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
     1381 ttcttctaaa gaatcaaaat atttttcgac agcaatcttt gaagctttta ctattgaagc
     1441 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
     1501 aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
     1561 tccttaatat aatttatgtt tttataatat cataataaga taaatttgca agcatttatt
     1621 tacacctttt tatttacact ttattataat taattagact tttatgattg catttttttc
     1681 ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
     1741 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1801 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta
     1861 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1921 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga
     1981 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     2041 gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac
     2101 gtttagaaga tcaatctgat gacgttttaa ccgctaatct agttttaggt ctgccttcat
     2161 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     2221 ttgaagttga cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc
     2281 cgattggaac actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat
     2341 tgatcagtca gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg
     2401 tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt
     2461 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     2521 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg
     2581 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     2641 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     2701 gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     2761 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2821 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2881 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca
     2941 ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag
     3001 aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagcagaa
     3061 gtaaagaata aaccaactga tcataaccct attcaacaag agcatccttc aacggttaca
     3121 tccgatcatt cagcaaagaa agaaaagcca atgctgaaag ggttcaatgc accaagtaac
     3181 aaagacttac tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac
     3241 aaatataaac ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat
     3301 aaacacgtat attaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg
     3361 atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac
     3421 caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc
     3481 tttgcgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta
     3541 gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt
     3601 caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt
     3661 tcacttcaat cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg
     3721 tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa
     3781 tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat
     3841 aattagataa cgttggtacc agttctagga actcacgact tgaattagta ttatgtttga
     3901 tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa
     3961 tcaactgttt tgctagtgca tcaatatctt taccaagctt cagcaaagct tcaaaaccat
     4021 ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta
     4081 gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt
     4141 catagttaac aaccatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt
     4201 gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttacag
     4261 ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt
     4321 tactgaaagc atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca
     4381 gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt
     4441 tacctgttcc acctttttca atgtttccag taatgattga caacttcttt tccatgatta
     4501 aacctccctt attaaccttt tgtaactcca ttatacacta taaacacgtt tatgtatatg
     4561 tttatataca taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata
     4621 tatatgtata tgtttatgtt tatgtatata ttcatatata taactattgc atttgtcata
     4681 caatcgtagt acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg
     4741 tgtatctaaa gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat
     4801 ttctgaattt atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac
     4861 ctacaaggaa ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat
     4921 gctaaaagag ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa
     4981 aagcgctaaa aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa
     5041 atcttgtagg cactgatgat ccaagaaaac atggtaaagg tctaacagct aataaatcag
     5101 gctactggcg atatagggta ggtgcctacc gtataatagc tgatattagc gagcatgaag
     5161 tgacaattct tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct
     5221 gataaggctt gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta
     5281 cctaaaaaag aacaataccc tcttgttatt atacacactg gtgtgtataa tataagtgta
     5341 aacaagctaa ttaactttca aaaggagcga ttttttatgg gatggtatgg tgcttattca
     5401 tcagcaaatg aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta
     5461 gataccaaag taacgaagac ctatggtatt atccttttca aagatgcaga aggaacctac
     5521 gatattgaca gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac
     5581 agtcctgctt actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa
     5641 gaaaaaatgg ttaagcaaca actagataga attaaagaag aaaacaataa accaaacctt
     5701 gatgacttat tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtt
     5761 tatcagtata aactcaaacg tagctttatt tttagactac aagatggatc actcactcgg
     5821 ttcactcgac tatcagcagg agatgttaag gaagtagcct aaaacttaaa tacataaaca
     5881 taaacgtgta tacgtttatg tttatgttta tgtatatgta tatgaatata aacgtattag
     5941 gtacgaacgt ttttaaatgc ttttctgggc tttgatttta gggtagggct aaaacatcca
     6001 ttgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactga taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 accggactag ccgtttcctc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacacta
     6301 gtgtgtataa tgtaattgta aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tagggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tagatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttgact tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg agggtagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtagtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaattact
     7861 tcaaaaagca ctagaagaag acttgcttga aaagtaaaaa ggagaccgaa cctatgaaat
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa ctcctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggctaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gttattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt ttttataagt
     8461 cagattttta taatcaacac ttagttttag ggagtttttt gcttatctac accattttga
     8521 tgcttaattt atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga
     8581 tcgagagaaa catcaaaagg gtatctttca agatcacgtt aaacaagcct accgataaaa
     8641 agctctcaga agcgttctga gagcttttga cattttcttg tataatttat cctaacacat
     8701 tacaaagcac ctaattcgag cttatatcaa gcaaatttgc tatttattcc tcaatacata
     8761 tctaaaaatt acttgatgga aaactagata catggtaagc atgaaaagaa aatcaatttg
     8821 agcagatgca gtataataca tagcaattta aggctctata ctttttgatg ttggtgaact
     8881 tcaccaacat cttttttgta t
//
LOCUS       DACCVU010000117         8888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCVU010000117 DACCVU010000000
VERSION     DACCVU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.118"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            161..877
                     /locus_tag="IVW29_002419"
     CDS             161..877
                     /locus_tag="IVW29_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7839880.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(937..2955)
                     /locus_tag="IVW29_002420"
     CDS             complement(937..2955)
                     /locus_tag="IVW29_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7839881.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2968..3966)
                     /locus_tag="IVW29_002421"
     CDS             complement(2968..3966)
                     /locus_tag="IVW29_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839882.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4164..5405
                     /gene="sstT"
                     /locus_tag="IVW29_002422"
     CDS             4164..5405
                     /gene="sstT"
                     /locus_tag="IVW29_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7839883.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5552..6187
                     /locus_tag="IVW29_002423"
     CDS             5552..6187
                     /locus_tag="IVW29_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7839884.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6378..7118
                     /gene="truA"
                     /locus_tag="IVW29_002424"
     CDS             6378..7118
                     /gene="truA"
                     /locus_tag="IVW29_002424"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7839885.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(7263..8861)
                     /locus_tag="IVW29_002425"
     CDS             complement(7263..8861)
                     /locus_tag="IVW29_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7839886.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2967 a         1408 c         1682 g         2831 t
ORIGIN      
        1 taaaagaagc acccgctgat gtattacaga caacatcagc gggtgcttct cagtttttag
       61 aagatttgaa agattgatga gaaaatacga aaaatcaata aaatcacatt gcttatttga
      121 tagaataaag aagtgggagt aatcgaaagg agttttttaa ttgaaaaaaa ggattttagt
      181 aacaattggt ctagcttttt tgttagctgg ttgtaccaac gggaatgaat cgaaaaaaga
      241 atcaacaaaa gaaacagcga aagcaacagt tgcaactact acaacaacta gcacagagag
      301 caagtcaact gcagcaagta cagaaacagc agatacctct acttcgtcag agtcttccaa
      361 aacgactgaa acaactcaaa catctacttc aagttcttct gtagaggctt catcttcctc
      421 ttcagaaagc agcgaacaag ttgaacaaac actgtggaat gcagacaaat ccgcacaatt
      481 agaggcattt atgaccaaat ggggccaaac aatgggacaa caatacaaaa gttatacgaa
      541 tcaaatgagt gtggacttat atggtcttaa agtccctcaa gctattttga acggggagtg
      601 gaagatggct attggtggag tgcctgtttc tgcagaatgg tcagaaagtg ggacaggtca
      661 agcagactat caaatcacag ccgtctattc cgatgcagag acagaaccat atctgaaaaa
      721 gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa gtattggtga cacaacaaaa
      781 tcaggggaat cccgataatt atctttactt caacgagaca gcgaataatg aattgaaaaa
      841 tggctttaat caaatcgttt atggacaagc aaattaatga gataaacaag gggttgtgac
      901 atgtctttta tcacaacccc ttgctcagtt tatttgttag tcttttattt tttgtgtcgc
      961 ccatgttgca ctgtactggt tacctgcaac aagatcatat tgtatcagac gataatcttc
     1021 tagaatttct ttttgtttct cagttaaatt tttttcttgg ataatttgac tatcctctgt
     1081 aacaaagacc tgttttccat taaaacgatt agacaagctt tctgtaggat caattgttaa
     1141 tgcaggtaga tgattagata cttctgtcaa caacgcatag taaggagtta ctttgatatt
     1201 cgcatgctca agagccatag caccaaagtt attaggagca acaacttctt tatttaacgt
     1261 agctgcttgt tcacgactat acttgttgtt ataaatgaaa taatctgtct ggtgctgttc
     1321 taatccatat tttttcgaat cattcccact atagattgcg ggtaaatggt caccataaaa
     1381 aacaaaggta atcggtttat cgattttgtc gatttcttgg atgaattttt ttactgctgt
     1441 atcggtatag tgaaggcctt gcatataagt ttgtaattct tttcttctat taggaatgac
     1501 agcagttcct tcagcagtgt agttcaattc actatagtaa tcatcataag gcatatggtt
     1561 ttgcatagtg gataattgga tgaattgtga cgtttccgta ttgctttgta aagctttcaa
     1621 ggtttctttg tatgctgaat catccgatat gcgtgggctt tgttctattt tatccgtata
     1681 agttaattta tccggactgt ctaaataata gaacctgtca aaaccaaatt tatcaaacac
     1741 ttgttttctg cgataaagag cagctgtaaa aggatggatg gctatttttt catcgaatag
     1801 atcggtaata tttggactta ttttctgacg ctccactagc tgggtataag gtgtaactaa
     1861 cgaagcagac aggttactga tactcaagcc tgtcagtcct tcccattcca tgttagctgt
     1921 tccgccacca taaccgacgg ataacatcaa tcctcctgtt ccttctgtta taatgctttt
     1981 gatgtaagga atagggtctg taggtactgt cagattaggg acacgtgcag gatcactgaa
     2041 gctttcactt aaattgagaa cgatcgtctg attgtttaac cagtcacttc ttgtttggtt
     2101 gatttcatct gcctttgtgt tgtacttttt cattattcca ttaatttttt ctttcgaata
     2161 gccagcaggt tcttccatga ttttaatgtc gatattgttt aggaattgaa tgactggtcc
     2221 gttatctttt acggcgcctt gctggttaaa gaaagtcttg tttactttaa ataaattaaa
     2281 cagcaagaaa gaaggcgaat ttttgtgatt aatgaaaaag acacccgaaa aacatcctaa
     2341 taatacagcg atactgataa atcgacgttt ccaatgaatc ttcaaggaat actgtttttg
     2401 tagtctacgt tgtacaataa tactagtaat caataatagg agaaggatca atccgcccac
     2461 gattaataag actgggctaa ccatcgataa tagttctgac aatccggtca gcatttttaa
     2521 atctgaagga aagacaggct cttcccgcat ttccattttg attactgtcg aaatagtgag
     2581 cagtaagtta atgatcaagg tagcagtcaa ggcgtaccaa aaacgattgg aaatcaacaa
     2641 tagtaagaag aaaaatccca taataatgat gattgtcagg atcatgggtg cttgtcgctg
     2701 agtaaatatt tttaaggcaa cctggatagt ttctttccaa ccttcttttg gttctaaaac
     2761 gaaaatctgc acgaaggtaa agcagtaaaa aaatgctgca actaaaaaat aacttttatg
     2821 aacaaaaagc caatgaattc tttttttcca acgctgagtt aattgttcaa tcgaatcaaa
     2881 agcgaagtat tcgatgaaac gatgaatgaa acggattttt tgtatataaa ataaaaagat
     2941 aactaggagg gacactgcta ataaatatca aataaaaaac tgcccgatat ttatcagcca
     3001 tgccttccct gagtttggaa attcacgttt cataaatgta gatacgcgat aagaaactaa
     3061 agggaaatta catataactt gagtgacaat caaaaataca gcaagaaaat ttcctcttcc
     3121 ttttatacct aatttatcag atagcatttc taaaaataaa aacgaagaga aagtataata
     3181 cattgcgaag agagaatatg gtttagaaaa cctcagcgct gtatctgtat ttcctctgat
     3241 gatgagtgaa atttgtgtca tcaaaataat caaaccgaat aaaacaatca agctgataaa
     3301 tacttgagga agcttatagc ggaccttttc tttccagttc cattctttca aaccataacc
     3361 cattaaaaat aaataaaaaa tccataaaat actctgtcca ttctgtaatg caaacgggtc
     3421 tttattaaat agagtagagc agccaactaa tccaattgtt aaaaataata gtagatattt
     3481 aattgttgtt ttcgataatt tatttaaaaa tccaactgta taaggaatac tacaaaaaag
     3541 caaagtacaa gaaacagcat aagtaaagta attctgactg ataggtgtta ggataagcca
     3601 ataatcacga atattcaagc tgccagcttg tacgtaaaaa aagatattgc aaactagtgt
     3661 ggctattagg aatataaagg caaaacgact taaataatgt aaaactttta aacgggcgat
     3721 tttttgatat tggtaaccca tgtaaagtgg gatcaaattt gcaccaataa caacggaagt
     3781 ttgtaaaaat gtatagatgt agaatcgacc agggtttgtt gcatatgtag gaatagtgac
     3841 tgctgaaatc aatatacgct gtaaaaccat cattatacaa gcgacaaaga ttgtcaggaa
     3901 gccataactc tccttcttta tttttttcaa gaataaacac ctcacaaaaa aattggacaa
     3961 aaacataaga aaacgtttcc tgataagttg cccattaata acatcctaac ttagaatatc
     4021 tcaaaaaaca acgtgtagac agaaaaagta caaaattagc aagaaatggt tgtttgtagt
     4081 gttagaattt ttagaaaatt atacctttag tttctttgca taaaatgata caatgattgt
     4141 taaaattgtg aaagggtggt catttgttag agaagttttt ggatatgtcg ttagttcaac
     4201 gtattgttgt tggtatttta gtcggaatat tgtttggtat ctttttcccc acatggacat
     4261 ttatttcaat attgggaacg ttgtttgtca gttgcttgaa ggcaatcgca cctattctgg
     4321 tttttttctt gacgatggcg tcaattgcca agcataaaat tgggaataag acattcgtga
     4381 agcctatttt gatcttatat ttagtgggaa cattgctttc tgcacttgta gcagttattg
     4441 taagttcaat ttttactgtt cctattactt tacaagaaac agtatctgaa aaagcaccgc
     4501 aaagtttgga aagtgtttta tctactatgt tgacgaatgt cacacaaaat cctgttcaat
     4561 ctcttattga tgcgaattac cttggcgtct tattctgggc tgtattgttg ggactagctt
     4621 ttcgagcacg ttcagaatcg acaaaagagt tggtggatca gatatctata gcgttgtcac
     4681 aagtcgtaca agtgattatt gcatttgctc cgatagggat tttaggattg gtttatcaat
     4741 caattgctac gactggaatc gctggattgg cagagtatct gcagctttta cttgttttga
     4801 tcggtacgat gctgtttgtc gcattagttg tgtacccatt cctcacgttt ttgtttatta
     4861 aagaaaatcc ctatccattg attttctttt gtttgaaaga aagcgcactt cccgctttct
     4921 ttacccgaag ttctgccgca aatatcccga taaacatgat gttggctgaa agattgaaat
     4981 tgacaaaaga gtcttattcc atatcgatcc cgttaggtgc tacgatcaat atgggcggtg
     5041 ccgcgattac catttccttg atgacgttga ctgctgtaca ttctttaggg attgaagcac
     5101 cttttgtttt gaaagtcctt ttatgtattt tgtctgcttt agctgcttgt ggagcttcag
     5161 gtgttgcggg cggttcactg ttattgattc cattagcatg tagtttgttt ggtatctcta
     5221 atgatgtcgc gatgcaaatc gtcggtattg gatttattat cggtgttatc caggattcaa
     5281 tggaaactgc cttaaattcg tcaagtgatc tgttgtttac tgcaatagga gagctaggcg
     5341 cgcgtaaaag aagtggggaa aaaatcaaat tgaaagattg tttagatgga ttaagtgaac
     5401 gttgaacgtt agatatttag tatttttaaa atgattcgag tttttaatga gttatataga
     5461 tgttcgtgta aatagaggtt tctgttaaat caagataaag tcgtttataa taacaattaa
     5521 aagcataggt aattgttatt ggaggcgtat catggatttt ttacgtaaat ttggtttttt
     5581 ctttttatta ttaggcgtgc tgagtgattt tttgactccg tacgtattag gattgtatta
     5641 tcctgaatta gatcaaatga agagcgtgat cagtgtattt ggagaggttt ccagtcctgt
     5701 aagaaaagcg tttcttattt ggtctgtggt ttcagggatc tttttcatcc tgtcattgcc
     5761 tgcattgtat gctgcatttg ttagtacatc tcgtgtgtta gctaccctag ttacagcagc
     5821 catcgcgttc tatggtatag gtgattgtat ctttacaggt ttgttcagta tcaataccca
     5881 tgaatcttcg tggaatgtct cgacatgggt ccataatatc ggatcaggtt taggttacag
     5941 tggtttttta ttgtttccag ttcttctttt cttgatctat cgaaaaaatg ggcaaatgga
     6001 cgtaagccac ttataccttg gactgtctgt gatcagtttg ctttttgcgg cgatttatgg
     6061 actggcaaga attccttcta taaattcttg gccattactg aataaaatag gtttttgcca
     6121 aagaatcagt tttttcttta attatgtgcc cattgcctgg ttgagtatct tgcatctcaa
     6181 acagtaaaac ttgagccgtc aaattgaaca gtcagcggat acttcatccg gtggctgttt
     6241 tttgtttttc tgaaatatat atttccacag ttttcttgta tagcttctgt cttagagaag
     6301 caaggagcta tattttgtgc tacaatctta aaaaggtaga gagaagacca aatggaagac
     6361 agaggagcca ctaaaatatg agaaacatca agttgacaat cgaatatgac ggaaaaagat
     6421 attcaggatg gcaaagactt ggagatgacg ataaaaccat tcaaggaaaa atcgaaaaag
     6481 tattgcatca gatgacaaat gaagagatag aaattattgg atcaggaaga acagatgctg
     6541 gcacccatgc gcgaggacaa gtagcaaatt ttaaaacaaa cacagaaatt ggactggcag
     6601 aaatgatcga ctttatgaat cgttaccttc cgcgggatat tgtcatcaaa agaatcgaag
     6661 aaatgcctga gcgcttccat tcaagatata atgcggtggg caaaaaatac agctattatg
     6721 tttggaacaa cgtgattcct tcagcatttg agcgaaacca cagcttttat ttccctcaag
     6781 agttggatat ggacaagctg aatgcagctt gcgaaaaatt agtgggtaca catgatttca
     6841 ttggtttttc tgcattgaaa aaatcaaaaa aatccacagt gcgtacgatt gatgaattat
     6901 ctatcgaacg aaagggagaa atgcttcatt ttacttttgt cggagacgga ttcttatata
     6961 aaatggtgcg gattatcatg gggacattac tagaaatcgg cacaggtgcc atgccgatag
     7021 aaacaatcga tgaaatattt gaaagcaaag tgcggcaaca tgcaggagaa accgtccctg
     7081 cacaaggtct gtttttggat gaagtttatt atcgataaaa atactttgat aatggatgaa
     7141 acgcatgttt cacagtataa atatcgatgt taaataatgt atgaagcaaa ttaaagaaat
     7201 ataaaaaaag actttttagc ctgtttatgt gtacagctaa aaagtctttc ttaaataaaa
     7261 atctagtgag aatgttgtac ttctcttctc ggttgttttt gtttagaaaa agtaaatcga
     7321 taaagtaaaa ataaaatcaa agtaacgata gataaatatc ttgaagcaat tgctagtttt
     7381 gtgtactgat aagtaataac gactttattt atacctggag gaatagttaa ttcagttgtg
     7441 ccaaacttag ataattttgt ttgtactagt ttgccattaa tagaagccac ttgcccttta
     7501 taacgataaa taggtgtcgt caagacagta tttttattat tcgaattatc taattcaatt
     7561 gtaagtttgt tactcataaa atgagttgtt ggatttattt cctggttacc tagtaaatat
     7621 tgatcattat taatcatatc aggatgatag attgactctt tgtttgcata atcagtatga
     7681 ttgtaatttt cagctatagc aacagcgtct tcaggtgcta taagtgttaa tgaatctttg
     7741 ttagcatgga tagaattgct tactcctgag agatgcaata ttagtgcaag aaacgtaaag
     7801 accattattt tccatgaatt atttttagta gaaaaaatat aatagatcac tatactcatc
     7861 gtataggcta taaataaaga agaaaaacca tttaaccgcc agacaaattg tagactctta
     7921 actggtgtat ggttaaaaag ttgccaagga aataaattgg ttgaacaaaa aagaacaaat
     7981 acaccaagaa agaaaataaa taaatcatca gcagttaatt tttttataaa aataaaagca
     8041 ccaatcaatc cgagaaataa gaataatcca atagtatagt tgttatagtt attatttaaa
     8101 atatgtgtca gcatatcact aggagccata ccacttaatt cttttcccag tgggactttc
     8161 aattcttgtg ctctagattg ttgaataaaa ggaattaaga atcccgcact taaaaaaaag
     8221 gtgaggacgg tagcttttaa aagacttaag agtctagcga ttttttggtc ccaaaaataa
     8281 aaagaaagaa acaacgttcc tccaataaat acagaaacca tagcgacaga aagtaaatga
     8341 gtatacacta ctagcgtcat gccaatagac aaccagtacc atttttggta atctctaata
     8401 taaatttcgt aacaccccat tagtatcaaa ggcaaaaatg ttaaagcgac agcttctccc
     8461 aatgaagcac gatggaatat gtcaatggca cgataggcgg aaaatgtata aataatcgaa
     8521 aataacagcg aaatataacg attgtttttt atttgtttca tagaaaaata agaaacgacc
     8581 attgttaaaa aagtgataaa aaaatagtag atattataac ttaaaacaag atttcccatc
     8641 attttataaa ataaaaaagc tggataaaga gtaagccatg gataaaaaat attcatcatt
     8701 gttccatgat gatcgaagtt attgaagttt actggagaac tccatacatt atctagtcct
     8761 atgattcgag ataaatgaaa gtaagtatcc tgattgacac tatgatagat tcccttagac
     8821 atgaatagag ggatgacgta agcaaaactt agaaataaca taaaaatgat atctattatt
     8881 tggttttt
//
LOCUS       DACCVU010000118         8836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCVU010000118 DACCVU010000000
VERSION     DACCVU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.119"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            117..764
                     /locus_tag="IVW29_002426"
     CDS             117..764
                     /locus_tag="IVW29_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839887.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(857..3136)
                     /locus_tag="IVW29_002427"
     CDS             complement(857..3136)
                     /locus_tag="IVW29_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7839888.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3414..5237
                     /gene="cadA"
                     /locus_tag="IVW29_002428"
     CDS             3414..5237
                     /gene="cadA"
                     /locus_tag="IVW29_002428"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7839889.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5284..5463
                     /locus_tag="IVW29_002429"
     CDS             5284..5463
                     /locus_tag="IVW29_002429"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7839890.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5800..7740
                     /gene="thrS"
                     /locus_tag="IVW29_002430"
     CDS             5800..7740
                     /gene="thrS"
                     /locus_tag="IVW29_002430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7839891.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7964..8812
                     /locus_tag="IVW29_002431"
     CDS             7964..8812
                     /locus_tag="IVW29_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7839892.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2779 a         1573 c         1880 g         2604 t
ORIGIN      
        1 attgtcttgc ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag
       61 agcataagta tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg
      121 aagcaaaaat cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag
      181 agttagctgt gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg
      241 ttgaaggcta tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt
      301 gtatggtgga ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt
      361 ttcgtcgttc tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct
      421 ttctactatt agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg
      481 agaagataga cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat
      541 cagagatttt ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca
      601 ttgaatggaa tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg
      661 tatttgaccc agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca
      721 aattaaaata tcctgaaaca gatttggatt tatatataga ataaatgacc ttaaatgatt
      781 gaattacttt ttctgcaatt attattgtaa ataaaaagac aactgctaac attaatgata
      841 gcagttgtct ttttacttac agtacttttt tctcataaga tgaaatatcc atattgttta
      901 acaaaggact ttcattattg gaggtaaaaa gggtcaattc atgcatcgca cgtgtacaca
      961 tggtgtaaag gatcaatttg tcttgctgag aagaaaaatg ttctgcatct acatcccaca
     1021 agaaaactcg gtcaaactcc agaccttttg ctaaaaaggc tggaagtaaa ataatggaac
     1081 gcttcataaa gtcactctca tttaaaatca gctggacttg ttgtttcaac tcgtctggca
     1141 gttgagcata aagagcttcg cattcttctt gtgttttagc tatgattgct gtacgccaat
     1201 accgggcttt tccctgagtc gttcgaatgg tatctgctaa ccaattcaat ttgatatcat
     1261 ctgttgttcc ttggatgagc aaaggaaggt ctccttttct tgctgttgtt tctacttgat
     1321 gttctttcac aataaaatga ttagcaaaat cagtgatttc ttttgtggag cgatagcttg
     1381 tcgttaattg gaaacgagtg acttcacggt ccacgaaaag gtcttccaat gatccaacga
     1441 tcgtttcatt tccaaagaca ttttgattta gatctccaca gaaagtcaaa tttgcttttg
     1501 ggaacaaggc ttgcagcaat gccacttgtg atggtgcgaa atcctgcatt tcatcaataa
     1561 agatatagcg agctttgtgt gtaaccgtca ctggatatgt tcggcgcatc agtaaaaaga
     1621 atagtacagc atcctcttga cgcaattctc tttgacgcaa gtgtgtacga acagttgtca
     1681 aatgttcctg ccacctggtc tcgctgattt tatgtcgtgt tagaagagaa agtggcacag
     1741 tttgtagaaa gtggagatat tgtttcgtta tattgacaaa ttgataattc atgatccctc
     1801 gatggattcg acggaatctt ttacgtacga tttggttggc cagtttttta cgtaatgaac
     1861 gctccttttg ttctgtatat tcaagatttg gatctgttgc gtatagttct tggagctgtt
     1921 cttccgccag atctttgacc cattgctgac gtgtttcgtc tttttgcaga ccgcctaatt
     1981 ttttcaggag cttcgtttgt aatagagaaa gtctatgatg taaaggtaat tgttcatttg
     2041 tttctttata ccattggcga atcgattcct ttgataggat cgtacggcca tttattttca
     2101 tatcacggaa caagggaccg tagtttgtga tgccttggat atagcgatca atctgatcga
     2161 ctaaggtcaa tccgcttttc atcacttgta tcggatcttt ttcccctagc aaaaaaccac
     2221 tttcttgttg ctgttcatct agtaaagaaa actctggcag cagctgcacc aaataatttt
     2281 tgaatgtctg tgtaggcacg ccgctttcac ctaatgaagg caatacagtc gagatgtaat
     2341 cagaaaacaa atggttgggt gaaaatagaa gaacgttttc agcctcgagc cacttgcgat
     2401 tatgatacaa cagaaaagca atacgttgaa gcaaagcgga tgtctttcca cttccggcga
     2461 ttccttcaat cagcattact ttacttgttg tgtcccgaat gattgcattt tgtgcttttt
     2521 gtatcgtaga gacgatgttt ttcatatggt tgcttgatga ttcatccaat atctctagca
     2581 aaaagtcatc attgattact tccgacgtat ctaccataga taaaatacgt ccttcttgga
     2641 ttttaaattg tcgtttcaac tgcagatcga catctattct atctgtttca gtttcataaa
     2701 atgcgggacc aatctcacct tcataataaa gattggcaat tggtgcgcgc cagtcaataa
     2761 cgattgtttc ttctgccaga tctcttaaag aagcgatacc tagatataat gtttctgttt
     2821 cacggtcttc tttaaagtcg atacgggcaa aataaggact atctttcatg acagacaacg
     2881 tttgcaggcg tttttgctgt gcttcaagag tatggtattt caaaagcaat tcctgctcat
     2941 gttggcgata ttcaacagca gattcataaa aagcttcatt ggaaccagct cgaatcttgt
     3001 gattagctgt gtcttttgtc gaatgattca tttcctgagt caattcttgt tgttttgtat
     3061 ccagcatatt ttgttcgatc tggattagtt cgatcgtttc atccacgtgc gtttgttcta
     3121 gttgtttctc attcatcgca tgtttgctcc ttccgccgta aataacgaat taaaagtata
     3181 gcatagtttt tcgaaaaaag gtacttttta actgatgaat cgaaaaagta atccttgctg
     3241 aatatttttg tttgtagaac tgatgaattt tggaaacgct gatagtgata gttgttttca
     3301 attaaggcct attgttttaa aaagtgctag tcaaggacta tgcttaagat gaaagaggca
     3361 gctggaaatt tattactaac ctctctaagt cacataaaag aggagaaaaa acaatgacac
     3421 atttcaaaaa atttttactt actttgctta ttggtgggat agctttatta agtgaatttg
     3481 tttttcacca gccggccctt gcatttgcga ttgtcgcatt tactggaggg attctcgcct
     3541 tcttgatgtt tttggaaatg atcaagacgt tacgttcagg aagatatggc gtagatgtat
     3601 tagcgattac cgcaatcctt gcaacattag ccgtcaaaga atactgggct agtttgatga
     3661 tattgatcat gcttacgggc ggagatagtc tagaggatta cgcgaataaa cgagcgggta
     3721 aagaactgca gtctttgtta gataacaccc caagaattgc ccatcgattc aaagaaggaa
     3781 aattgcagga tatagtaatc gatgaaataa atattgatga ccagttacta gtcaagcctg
     3841 gcgaacttgt acccgcagat ggtcgagtga ccaaaggaga atcttcggtc gatgaatctt
     3901 ctttgacagg tgagtcccgc ccggaaacca agaaacctgg agatcagatt atgtcagggt
     3961 ctttaaatgg agacggatcg atccaaatga tcgctgaaaa acgtgccgca gacagccagt
     4021 atcaaatgat tattaaacta gtcgaagaat caaaagaaaa gcctgcccga tttgtccgtt
     4081 tagctgatcg ttatgcagtc cctttcacgt tgattgctta tttgatcggt ggtatttctt
     4141 ggtggttctc aaaagaccct ttacgatttg ctgaggtact ggtagtggca tcaccttgtc
     4201 cactgattct agctgcgccc attgctttag ttgcaggaat gagccgttca agtaaaaacg
     4261 ggatcgttgt aaaaactgga acaacgattg aaaagctcgc acgtacaaaa acgattgctt
     4321 ttgataaaac aggaacgctt accaaaggga tattggaagt agaggcgatt cagccagtca
     4381 atggaataaa aaaagaagag ctacttctgt atgcagctag tgcagaacag gaatctagcc
     4441 atatcttagc tcgttcgtta gtgaacaacg tgaaaaacag ccaactgtat cctgttagtc
     4501 aactgaaaga agtgaccggt caaggaatcc aagcagtagt caataatcat gaagtaaaga
     4561 ttggacgtgc ctcttttgtt tcagctgaag aaatagaaac gtccaataca gcaatctatg
     4621 taacaattga tcgatctttt gcaggaacga tcctattttc ggatcagatt cgcccagaag
     4681 cagcaaagac aatcaaggag ctaagaaaac ttgatgtgaa aaatttgatg atgatcaccg
     4741 gggatggatc tgccattgct caaacgattg cagaaaaagt ccaattagat tctgtacatg
     4801 cccgtagctt gcctcaagat aaactgacca ttttagaaag tatccctaaa gatcaaagac
     4861 ctgttgcgat ggtaggagac ggcgtgaatg acgcaccagc acttgctgct gcagatatcg
     4921 gtattgcaat gggggctcat ggttctacag ctgctagtga aagtgcagat gcggtcatct
     4981 tgaaagatga tctcttgcgt gtagcaaaag cagtagtgat ttcaagagat acgatgagaa
     5041 tagcgaaaca gtctgtactg attggcatct ttatttgtat ttttttgatg ttaattgcaa
     5101 gtacgggact aatccccgca ttattcggag ctgttttgca agaagtcgtg gatacggtct
     5161 ctattttgag tgccttgaga gcaaaaaaag atccagccat ttttcctgat tcagtaccca
     5221 agacaaacat agtatgatag aatcgagttg aaaaaagaac gatcagaaag aggagagagc
     5281 attatgccat tcgtacatgt cgaattagta gaaggtcgca gtccggaaca attagaaaat
     5341 atgatgaaag atatcacgga agctgttcat aaaaacacgc aggcaccaaa agaacacatc
     5401 catgtcatca tcaatgagat gaaaaaagga acatacggtg taaatgggga atggaaaaaa
     5461 taagtttagt ttcccacttg atttttctat ttattagtgg ttaaataaac acataattga
     5521 ttgatgagaa agccaactga atttgagagt cttcgataag agaggaaaag ctaggctgaa
     5581 acttttccag gagaaaacca aaggatgacc actttcgaaa cccttgttga agcaagggcg
     5641 gtcggaccgt tacaacgctt gaggaacgca tttgcgtttg aacattaggt ggaaccacga
     5701 acttttcgtc ctagtatctc ttttcaagag atgctaggat ttttttgttt ttcagcttga
     5761 tgatctgatc aatcaaaaca tttcaaggag gaaactatta tgataaaaat tacttttcca
     5821 gatggtgcag tcaaagaatt tgagtctgga acaacaacat tggcaattgc tgaaagcatt
     5881 tcaaaaagct tagcaaaaaa agctttagct ggtaaagtga acggaaaact cgttgatttg
     5941 actcgtccaa tcgaggaaga tgcaagtatt gagatcatca caccagacca tgaagatgca
     6001 cttggcttag tccgtcattc agcagctcat ttgatggccc aagccatgcg tcgcctctat
     6061 ccaaatatcc actttggcgt tggaccagct atcgattcag ggttttacta cgatacagat
     6121 aatggacaaa accaagtaac tgcagaagat ttgccggcaa tcgaagcaga aatgatgaaa
     6181 atcgtcaaag aaaatctgcc gatcgaacgc cgtgttcttt caaaacagga agcattagaa
     6241 atttttgcaa gtgatccgta caaagtagaa ctgattagtg aattgccaga agaagaagtg
     6301 attactgctt atcaacaagg cgaattcatc gatttgtgtc gtggaccaca tgttccatcc
     6361 actggccgta tacaagtatt taaattatta tcagtggcag gtgcttattg gcgtggaaac
     6421 tcaaacaatc aaatgatgca acgtgtatac gggacagcct tttttgacaa gaaagctttg
     6481 aaagaatata tccgtctccg tgaagaagca aaagaaagag accatcgtaa actaggaaaa
     6541 gaacttgact tgttcatggt ttccccagaa gtaggttctg gtttgccatt ctggcttcca
     6601 aaaggggcaa caatccgacg cacgatcgaa cgttatatcg tagacaaaga agttagtcta
     6661 ggttaccaac atgtatatac accaataatg ggagatgttg agttgtacaa aacatctgga
     6721 cactgggatc attatcaaga agacatgttc ccgccaatgg atatgggaga tggcgaaatg
     6781 cttgttcttc gcccaatgaa ctgccctcat cacatgatgg tctacaaaaa cacgatccat
     6841 tcttatcgtg aattaccaat tcgtatcgct gaattaggaa tgatgcatcg ctatgaaaaa
     6901 tcaggtgctt tgtcaggttt acaacgtgta cgagaaatga cgttgaatga tggtcataca
     6961 tttgttcgtc ctgatcaaat caaagatgaa tttaaacgta cgcttgaatt gatggtggca
     7021 gtatatgctg acttcaatat tacagattat cgtttccgct tgagctatcg cgatccaaac
     7081 aatacagata aatattttga tgatgacgca atgtgggaaa aagcccaagc aatgctgaaa
     7141 gccgctatgg atgaattaga actagattac tttgaagcag aaggcgaggc agccttttac
     7201 ggaccaaaac tagatgttca agtcaaaaca gctcttggta cagaagaaac attatcaact
     7261 atacaattag acttcttatt gccagagcgc tttgatttga catatgttgg agaagacggg
     7321 gaaaatacgc atcgtccagt agttatccac cgcggaattg tttctacaat ggaacgtttt
     7381 gtcgcttatt taacagaagt atacaaaggc gcttttccaa catggttggc acctatacaa
     7441 gcaacaatca tccctgtttc tgttgatgca catggcgatt atgcttatga aatcaaagaa
     7501 cgtttgcaaa tgaaggggtt gcgtgtagaa gttgatgatc gtaatgaaaa aatgggctat
     7561 aagatacgtg cctctcaaac acaaaagatc ccttaccaat tagttgttgg agacaaggaa
     7621 ttggaagatg caacagtgaa cgtacgtcgt tatggaagta aagaaacagc tgtagaagat
     7681 ttgaatatct ttattgatgc tatggaagca gaagtcaaaa attacagcag agaaaactaa
     7741 taggtaaatt tcctgtaaaa aagtagtaga tagaaagcaa tccgaacaat agataacagg
     7801 tattcctcaa agacacagat gctcaataga atctgtggga ttgatgggat aagatattct
     7861 gttgggcgga ttgttttttt gttttttatc cagtatacga ccgtgaataa gggtctgata
     7921 tggtatactt acatagacta tcattaaaag ggtggaggaa cttatgagcg taattagtcg
     7981 tattgaaagt atgatggaag attattcttc agcagaaaaa aagttggcca actacattat
     8041 cggtaatatt gagaaggtcc ctacaatgac agctaatgaa ttagcagaag cttccgggtt
     8101 gagtgcgccg acagttgtac gtttttccaa aaaaatcggt tttcaaagtc tgacggactt
     8161 caaaatcacg atttcaacag aactacagac tggaatcgat gaaggtttta gtgatataga
     8221 agcaaatgag tccttttact ctatcaaaaa caaacttggt aacaatgcac aagtagcgat
     8281 aaaagaaaca gtagatattc tagaagaaga aacgatccaa aaagtcgtag agtgtttgga
     8341 atcggcagag actgtttttt tatatggggt tggcgcctct tctttagtag tagaagatat
     8401 tctgcaaaaa tggtctcgtg tcggtaagcc aatcattttt gaaaaagata tccacgtctt
     8461 gctcccgcaa cttgtcagca atgagaaaaa gaaagtgctt tggcttgttt caaactcagg
     8521 tagaagtgca gatgtagtcg cattagccga gctggcgaaa agcatgaata tcgaaatcat
     8581 tgcgttgacg caatttggaa ataacccgct tagcaagata gccgatgtac tcgtacaaac
     8641 atctagacca aaagaaatca cgaatcgaag tgcggcaact aattcacttt tggcccaatt
     8701 tgctacgatc gatatcattt tttatttcta tatggctaaa aacgaaaagc ttagcgaaaa
     8761 agtgaaaaaa acacgagaag caatccaaga atattttatg gattcgaaat aaaaacctta
     8821 ctatttttta tataag
//
LOCUS       DACCVU010000119         8653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCVU010000119 DACCVU010000000
VERSION     DACCVU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.120"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(251..2632)
                     /locus_tag="IVW29_002432"
     CDS             complement(251..2632)
                     /locus_tag="IVW29_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7839893.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2622..3269)
                     /gene="recU"
                     /locus_tag="IVW29_002433"
     CDS             complement(2622..3269)
                     /gene="recU"
                     /locus_tag="IVW29_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7839894.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3333..3872
                     /locus_tag="IVW29_002434"
     CDS             3333..3872
                     /locus_tag="IVW29_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7839895.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3962..4381
                     /gene="gpsB"
                     /locus_tag="IVW29_002435"
     CDS             3962..4381
                     /gene="gpsB"
                     /locus_tag="IVW29_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7839896.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4403..4770
                     /gene="rnpB"
                     /locus_tag="IVW29_002436"
     ncRNA           4403..4770
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVW29_002436"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4979..6145
                     /locus_tag="IVW29_002437"
     CDS             4979..6145
                     /locus_tag="IVW29_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7839897.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6191..7687
                     /locus_tag="IVW29_002438"
     CDS             6191..7687
                     /locus_tag="IVW29_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7839898.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7768..8451
                     /locus_tag="IVW29_002439"
     CDS             7768..8451
                     /locus_tag="IVW29_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7839899.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2720 a         1570 c         1838 g         2525 t
ORIGIN      
        1 ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa gtaaatggat
       61 aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta aatgttagat
      121 cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt ggtacatgat
      181 ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg tagacgtggt
      241 ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta tggtcatttg
      301 cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca gatgaagacg
      361 gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg
      421 tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga ctagtagagc
      481 tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca tagctggaag
      541 atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga tctttgtagt
      601 acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta tttgtcacgt
      661 tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt gacgattctg
      721 aagtgacagg tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa taatttggcg
      781 tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt tttgcatact
      841 cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca gcgatttgcg
      901 cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc atataagctg
      961 tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt ccgtcttgga
     1021 agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg aaagccgcat
     1081 aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt tcttccgtat
     1141 tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga ttttttaaga
     1201 aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga acattacggg
     1261 ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc cagtttccta
     1321 caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt ccagtagaat
     1381 atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat
     1441 cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc
     1501 cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag gcaacttgca
     1561 tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat agtttctttt
     1621 gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg
     1681 ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc ttagtattgt
     1741 catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca gcttgatcat
     1801 attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta tctcgtcttt
     1861 tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga ggcatcccag
     1921 caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag tacatctcag
     1981 aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg tagtaggtca
     2041 agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct tcttgggcct
     2101 tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg atcaattgct
     2161 gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg gcagaaccga
     2221 tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct accgacacga
     2281 tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc
     2341 ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg gtagcgtcta
     2401 attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg
     2461 ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata aataatcctt
     2521 ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc acactctttt
     2581 taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc attcttgctt
     2641 tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata aggaatccgc
     2701 ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa aggtagtgat
     2761 ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact attcaaataa
     2821 aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt ttgttcgagg
     2881 catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa agatgttttg
     2941 ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc attatagtct
     3001 gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct cttaggatag
     3061 tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa cccgcgggaa
     3121 agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg attgccgaat
     3181 tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt cttataataa
     3241 ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc acattatagc
     3301 aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta atctatgtgt
     3361 ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg
     3421 ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg gaatgggtac
     3481 ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg gaattgaaag
     3541 aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt ggtaataact
     3601 ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat tatgtcgaag
     3661 ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact cgttttatac
     3721 ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga aaaacgaagt
     3781 atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt cggctgatca
     3841 gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt tatcaacttt
     3901 tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa
     3961 tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca aaacaaaaat
     4021 gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca aagattatga
     4081 aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga gtgcaaaagt
     4141 agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc
     4201 aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga
     4261 agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac aacctaatcc
     4321 aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac gtcaatttta
     4381 atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga tcaggctgag
     4441 gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa
     4501 gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc tatgcttaag
     4561 tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt ggaacgcggt
     4621 aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac
     4681 aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt tctccctgga
     4741 aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat taggtcgtga
     4801 caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt caatacttca
     4861 gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg aatttgagta
     4921 aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga gattgtgtat
     4981 ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag aagcactggt
     5041 tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac gtgcacgttt
     5101 tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag cagatcgagt
     5161 aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa
     5221 agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag ctggaaaatc
     5281 gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt
     5341 tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg aaactggcgc
     5401 acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc tggatacgac
     5461 aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac cattgaaaga
     5521 aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc cattttatga
     5581 tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc ataatatcgc
     5641 accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc aagaaatcat
     5701 ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat tagatattac
     5761 tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag aagaaatcgg
     5821 attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga
     5881 atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag aagaaactgt
     5941 tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat ggagtaaata
     6001 tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa ccaaaaaaag
     6061 aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa ctcgtccgcc
     6121 tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg gatgtttgga
     6181 ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca atcttctaca
     6241 acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa aagctagtgc
     6301 ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa aaatcggtcc
     6361 aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt cggaagttgg
     6421 aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg taccggaaga
     6481 tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc aaaaagctag
     6541 agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg aattaacaaa
     6601 acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt tgctgaatca
     6661 atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc ttaaagaagg
     6721 aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa cagacttttt
     6781 aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa tcgaacaact
     6841 tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta tgactggaat
     6901 taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca aaatggctgt
     6961 ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg atgataaata
     7021 tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac aatcgttgtt
     7081 taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc ctttctttca
     7141 agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc gttcattgaa
     7201 ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc ctctccatat
     7261 catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact
     7321 acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa caaatgatgt
     7381 ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt ttccctctta
     7441 tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag aaatagatgt
     7501 ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt cagagcatat
     7561 ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc
     7621 gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg tatatcaggt
     7681 aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga tgttttcagc
     7741 tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc tgattatagt
     7801 atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa aagagttgtc
     7861 aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt gacgcttgca
     7921 gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag
     7981 caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat ctcgatgatt
     8041 ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc cgaactatct
     8101 ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc aatcggcatt
     8161 ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc tgaactgtat
     8221 ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat caatcatttt
     8281 tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata tgaagaagcc
     8341 aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga atggtctatt
     8401 gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg atggaggttg
     8461 tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt tacttctgga
     8521 acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt
     8581 caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa ttggcaggag
     8641 aacaccgttt att
//
LOCUS       DACCVU010000120         8589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCVU010000120 DACCVU010000000
VERSION     DACCVU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.121"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(48..1514)
                     /locus_tag="IVW29_002440"
     CDS             complement(48..1514)
                     /locus_tag="IVW29_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7839900.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IVW29_002441"
     CDS             1901..2497
                     /locus_tag="IVW29_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7839901.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IVW29_002442"
     CDS             complement(2555..3037)
                     /locus_tag="IVW29_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839902.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IVW29_002443"
     CDS             complement(3143..3340)
                     /locus_tag="IVW29_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839903.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IVW29_002444"
     CDS             3635..4393
                     /locus_tag="IVW29_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7839904.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IVW29_002445"
     CDS             4453..4881
                     /locus_tag="IVW29_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839905.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IVW29_002446"
     tRNA            4927..4999
                     /locus_tag="IVW29_002446"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IVW29_002447"
     CDS             complement(5149..5520)
                     /locus_tag="IVW29_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7839906.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IVW29_002448"
     CDS             5735..5920
                     /locus_tag="IVW29_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839907.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IVW29_002449"
     CDS             complement(6003..6191)
                     /locus_tag="IVW29_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839908.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IVW29_002450"
     CDS             complement(6215..6478)
                     /locus_tag="IVW29_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839909.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IVW29_002451"
     CDS             6636..6893
                     /locus_tag="IVW29_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7839910.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IVW29_002452"
     CDS             complement(6964..7155)
                     /locus_tag="IVW29_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839911.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IVW29_002453"
     CDS             complement(7296..7616)
                     /locus_tag="IVW29_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839912.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IVW29_002454"
     CDS             complement(7639..8073)
                     /locus_tag="IVW29_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7839913.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..>8589
                     /locus_tag="IVW29_002455"
     CDS             8408..>8589
                     /locus_tag="IVW29_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7839914.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2721 a         1695 c         1361 g         2812 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgagg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattc
//
LOCUS       DACCVU010000121         8452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCVU010000121 DACCVU010000000
VERSION     DACCVU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.122"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            376..1815
                     /locus_tag="IVW29_002456"
     CDS             376..1815
                     /locus_tag="IVW29_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7839915.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2110..2328
                     /locus_tag="IVW29_002457"
                     /pseudo
     CDS             2110..2328
                     /locus_tag="IVW29_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2577..2792
                     /locus_tag="IVW29_002458"
     CDS             2577..2792
                     /locus_tag="IVW29_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839916.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3009..3265)
                     /locus_tag="IVW29_002459"
                     /pseudo
     CDS             complement(3009..3265)
                     /locus_tag="IVW29_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3452..4363)
                     /locus_tag="IVW29_002460"
     CDS             complement(3452..4363)
                     /locus_tag="IVW29_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7839917.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4382..5386)
                     /locus_tag="IVW29_002461"
     CDS             complement(4382..5386)
                     /locus_tag="IVW29_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7839918.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5383..7506)
                     /locus_tag="IVW29_002462"
     CDS             complement(5383..7506)
                     /locus_tag="IVW29_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7839919.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7511..8431)
                     /locus_tag="IVW29_002463"
     CDS             complement(7511..8431)
                     /locus_tag="IVW29_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7839920.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2427 a         1722 c         1561 g         2742 t
ORIGIN      
        1 atcctatgga attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg
       61 tgcaacacca taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc
      121 acgagatagc tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag
      181 aagaaagtaa agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca
      241 acttacatgg ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt
      301 cgcgagaagt tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag
      361 aaaggaaatc ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg
      421 tggattttga ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc
      481 aatattgcac tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg
      541 gtagtgtccg tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc
      601 gatagccgtg ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg
      661 ctgttgtgtg ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc
      721 attggtgcta gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata
      781 catgaacgtg gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct
      841 ggaccaggtc ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc
      901 aatgtaccgg tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc
      961 accatgagcg gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc
     1021 agcttttttg gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt
     1081 gtactgttcg taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca
     1141 gcctctccat tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt
     1201 acagccttgt tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg
     1261 caaaatgctc gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg
     1321 atgatggtcg tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt
     1381 ttggtattag taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca
     1441 gtggactctc ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc
     1501 ttgttccagt cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga
     1561 ctggcaggct cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt
     1621 tctacgacgg tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat
     1681 atcgcaaatc ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt
     1741 gttttgtgtt ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt
     1801 cagaaaaatg tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag
     1861 tgggatttcc atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta
     1921 ttatcttcaa aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa
     1981 agccgctggc aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg
     2041 ttatgaatac ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa
     2101 gattttcgat acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa
     2161 attggttcag aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact
     2221 tgtcctattt gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc
     2281 aagggctatg aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga
     2341 tagcttggtt aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa
     2401 ttccgtggct ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat
     2461 ttaggttcct tttctaatta taataagatg accactacag taacctactg aaatatataa
     2521 acagccactg tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga
     2581 acgctggaat tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt
     2641 caatatcaaa aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa
     2701 gatcagtgga aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa
     2761 atggaaaagc tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt
     2821 agctgaattt aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt
     2881 gcaagcctat gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc
     2941 taaggctttt acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga
     3001 aagtctcatt ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag
     3061 gttgtgcttt gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa
     3121 tgtgatgtga ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat
     3181 caatgacatg tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg
     3241 aatgattatt tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg
     3301 acatggatta aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg
     3361 cattcatcac ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta
     3421 taacttctaa taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc
     3481 catttttttg taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa
     3541 ggtattttat ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct
     3601 ctaactccga aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat
     3661 actccaattc tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg
     3721 tgatttcttc cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat
     3781 attttgcctt gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt
     3841 tttcatcttc aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct
     3901 ctgtaatatg ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat
     3961 ctagaatttt tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt
     4021 ggattaaagc gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa
     4081 ttgcttcttt gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt
     4141 caacgccaga agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt
     4201 gatcaatggc ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc
     4261 aaagggcaag caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt
     4321 ttgggctctt ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct
     4381 cttatttgta tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg
     4441 tccaccccac tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat
     4501 gggtcacata agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg
     4561 catcttttag atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt
     4621 tcaggtcaac tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg
     4681 gacttgagcc accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt
     4741 cttggactat tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt
     4801 aaaacatatt gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg
     4861 ggtaatcgac tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac
     4921 tatgcttctt cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct
     4981 gaataatact atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat
     5041 agagacagcc ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg
     5101 actcagaact ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca
     5161 aaagaattgg aatatactct gttatatccg attctttggc atacttttcc accattgcac
     5221 ggtgtttcaa tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat
     5281 ttccctcact gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga
     5341 tggtcagaaa agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga
     5401 ccttgatttt ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag
     5461 agtggacctc tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc
     5521 ttttgaaatg atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct
     5581 cacaatccgc tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt
     5641 atctattgga atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt
     5701 gcgtttttcg tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg
     5761 atccgctttt tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc
     5821 gattccttta tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac
     5881 ttgtttagtc ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc
     5941 agccaaattc attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc
     6001 agatagtatt tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg
     6061 cttaaatttg gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc
     6121 aaaagccaaa gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat
     6181 ttgcggtttt cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa
     6241 catactcata atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa
     6301 agcaaccatg aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa
     6361 tgcagtcgta atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat
     6421 aatcgccttc tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg
     6481 cagaaacatc gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag
     6541 aaaaacaaag aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg
     6601 gttcatggtc ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac
     6661 cgcttcttcc cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac
     6721 ccggtcttca ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg
     6781 ttggacttga atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct
     6841 ttctgaattg ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga
     6901 gctaaagtca ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag
     6961 tagaaaaatc atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa
     7021 taatccggta tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa
     7081 ataaaatccg gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt
     7141 tccaatactt tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata
     7201 acccgtggcg ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag
     7261 accatacatc acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga
     7321 gctatccaca aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc
     7381 tgcttcaact gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa
     7441 tagaactatc agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt
     7501 tttcatcccc tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga
     7561 gttcttcaaa gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca
     7621 agcattggcc attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca
     7681 agccaaagaa ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact
     7741 tcaacccgat attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa
     7801 agtaaacccc ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc
     7861 cttgcgctac ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc
     7921 gattactatg gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa
     7981 gcaattccat ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg
     8041 ctacttgaat gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg
     8101 agaaaagcaa atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt
     8161 ccgcaactgg tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat
     8221 tgctttgggt aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac
     8281 tagaaattcc tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt
     8341 tctccataat cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga
     8401 gaatattgat ttcatccgca atttctggca aggtttcttt ccagccaccc cg
//
LOCUS       DACCVU010000122         8434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCVU010000122 DACCVU010000000
VERSION     DACCVU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.123"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..173)
                     /locus_tag="IVW29_002464"
     CDS             complement(<1..173)
                     /locus_tag="IVW29_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7839921.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVW29_002465"
     CDS             complement(593..1714)
                     /locus_tag="IVW29_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP7839922.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IVW29_002466"
     CDS             2042..3451
                     /locus_tag="IVW29_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7839923.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVW29_002467"
     CDS             3448..4176
                     /locus_tag="IVW29_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7839924.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVW29_002468"
     CDS             complement(4304..5215)
                     /locus_tag="IVW29_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7839925.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVW29_002469"
     CDS             complement(5232..6239)
                     /locus_tag="IVW29_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7839926.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVW29_002470"
     CDS             complement(6236..8365)
                     /locus_tag="IVW29_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7839927.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
BASE COUNT         2583 a         1708 c         1476 g         2667 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actg
//
LOCUS       DACCVU010000123         8289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCVU010000123 DACCVU010000000
VERSION     DACCVU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.124"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(127..1011)
                     /locus_tag="IVW29_002471"
     CDS             complement(127..1011)
                     /locus_tag="IVW29_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7839928.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1106..1543)
                     /locus_tag="IVW29_002472"
     CDS             complement(1106..1543)
                     /locus_tag="IVW29_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839929.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(1760..2671)
                     /locus_tag="IVW29_002473"
     CDS             complement(1760..2671)
                     /locus_tag="IVW29_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7839930.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3128..3556)
                     /locus_tag="IVW29_002474"
     CDS             complement(3128..3556)
                     /locus_tag="IVW29_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839931.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(3584..4222)
                     /locus_tag="IVW29_002475"
     CDS             complement(3584..4222)
                     /locus_tag="IVW29_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7839932.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(4226..6262)
                     /locus_tag="IVW29_002476"
     CDS             complement(4226..6262)
                     /locus_tag="IVW29_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7839933.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6388..7053
                     /locus_tag="IVW29_002477"
     CDS             6388..7053
                     /locus_tag="IVW29_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839934.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT         2658 a         1554 c         1255 g         2822 t
ORIGIN      
        1 aaatttttct attaggtgta aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc
       61 ttgtcacagt cccttatccc gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc
      121 tccattttaa caataaacct gtaaatgtga gggcaatact ttgattgtca caggtaattt
      181 atccccttca tctccatcaa cattcgtcgt caattcttcc tcatcagcta atgacacaac
      241 taattgctca caagtaaggt aggctagatt tttcgtagat tcatctactc cttttaatag
      301 gtctggcaat gttttaagcg tatcgatcaa agaagaatct tttaaataaa ccaaatggag
      361 cttaccatca tccacttttg catttggaag taatgtttca aaaccgccga ctgagttagt
      421 tagtcctatc agcaaggtac tgctctcaat ttcttccttc tttccatcaa cttctacgtg
      481 aaacgagtag gattgtgtgg aagctaactg tttgattcct gacatgaaat aagctagttt
      541 tccaaatttc gtcttcttct ccgcatctac atcattgatt gattcaggga tagagccaat
      601 tgcgacgaca ttcataaaat agtcgtcatt tatttttcca atatccaatg gcttgactga
      661 ttcaatcgaa aaatgattga tcgcttcctc aggttctagc ggtatcccta atgctcgagc
      721 caagtcattc accgtaccca atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc
      781 gattccactg attccttcgt tgactgttcc atctccgccc atgacaaaca cactatgata
      841 cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa
      901 gacgaccact tcatcaaaca ccgattccaa ttttgctatc gctaattgct caaattcctt
      961 tgcttgttca ccgcctgaac tggggtttac cactaataaa acttttttca tcactttctt
     1021 tcccttctga ttgatttctt taatataacg atagcataaa gaaaaaaatt aggctgtgat
     1081 gaagtactgt tcatgaaaca ttcatttatt ttttgacaag ataaaacatc catactatga
     1141 aactgaggaa aattaaaaca atgatatagg aaataaaaga aaatgatagg cattgtatac
     1201 ctaataaagt aaaaatctta ggtggcagat aaaaacacaa aaatagcaag agaatgtaat
     1261 agcgtttctt ccactttttg ttatcagacc ttatagcgat cagaggaata ccaaatgcat
     1321 aactgaaaaa ctcttgatgt actgttaata tgtcacggcc ttgaataata aatgcgacaa
     1381 tccccgctat aattagtgtg cctaacacca aaacggaaaa ttgatgaata tattttatat
     1441 acaaatgatc cattgtactt cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt
     1501 gaataacttc tagaaataaa agaaatttgc aaaaccactc catttatcta cttctacgtg
     1561 cattttttga tattgttttc tttatttaag attatacaga aaaatatcta gggtcgctgg
     1621 attctaagta aaaaaaattt ttaaaataaa aagaactaaa tcaagtagta attaaactac
     1681 tgttgattta gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca
     1741 tattttttat ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac
     1801 tcaaaacatt ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc
     1861 caattacttt attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca
     1921 attctttttt gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca
     1981 cataatattc cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac
     2041 catctttcaa cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat
     2101 caaatttcac ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta
     2161 atttagagaa atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac
     2221 ttgctacttg gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta
     2281 aatcttcatc ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt
     2341 tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg
     2401 aatcacccaa ttgtttcttt gtttgatcat attgagaatc gatttctttg gttgatacgt
     2461 catctccata tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg
     2521 ctccttgcat ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat
     2581 aactaacggc tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta
     2641 cgatggcaag tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg
     2701 cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct
     2761 gctcgatacg tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat
     2821 aaatgtcaag tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt
     2881 ctatcttgcg ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt
     2941 tttcttcttc cgctgctggg atattcaaat cagacacttt tatgttcgat tttccagaca
     3001 tttttttgtt gtttttatta tgatatattt ttatacaact aaaaatgaat atattaaaac
     3061 taccaaaaat aacttttaac gctgtttcat tatatgtttt ttctatttta tataaacttt
     3121 tggatactta ataccaaccg gttggattgt accaacatga gaatgtgtca gttctatttc
     3181 ctgtcgcact tgcataggcc catactcctt ttgttgcatt tgctgatcga atagagtttc
     3241 caactttcgc ttttgcaccg tgacgattat agacacgact atagtggtta gaatgacctg
     3301 tttttctcgt acaagttgta tcactataat aaacccatct tccagagcta tgattaattg
     3361 ctgttctaga aatttcccgg tctactggta aatttgactt aataatatta cctttagcat
     3421 caaaatgaat ctctcctggc tttaaagatt tgtaatagat ttcaccgtga actgaatttg
     3481 cttccacttc agatgacata gcgtttcctc caacaaaacc tatccctagt gcacaaataa
     3541 taactgcttt tttcataaaa cgacatcctt tcctttttga tttttaaagc atgatcttct
     3601 tgtttcctat tttggtcaga tatggatcat gcgtgactac gataactaac gtaccttggg
     3661 aagcttgttg cgataagatt tcaactactt tatccttatt tcttttatcc aatgacccag
     3721 tcggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca caggcgattc
     3781 tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg tttatattta
     3841 cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa ttcgaaaact
     3901 tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca aaatcttgaa
     3961 aaagaaaaga tatggtattt ctttttatca agcgcttctc tctcccagtt aattgtttcg
     4021 tctcttgatc gaacaaatag aattgtcctg aactgaactg atcattcaat cctaatatat
     4081 tcaataacgt acttttacct gctccacttt ctccaacaac agtcacaaga tccccagcat
     4141 ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt ttcgcacctt
     4201 ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac tgacttttct
     4261 tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta aagaaaaaaa
     4321 tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca cttgtcgttc
     4381 cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc gaataaccac
     4441 ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat aacatgacat
     4501 agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag taatgaaatg
     4561 atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat gataactgca
     4621 agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc gacaaagagc
     4681 ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt tccaaataca
     4741 tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag gggatagaag
     4801 gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt tctttttcac
     4861 tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct gtatgtctat
     4921 atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg atcaaccttc
     4981 cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt atccctctgc
     5041 caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata cttccgtttt
     5101 tattcaaagt ttcatataat tcctgatctg ctgctgatgc aaaacgattg gaataaataa
     5161 aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc tcaccagcga
     5221 tttcttttgg tggttcatgg gaaacagcta actgtgaaga agaaaaaacg atagttgcta
     5281 attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta aaaagaatgt
     5341 attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat aaaagagaaa
     5401 tcaaagagat accatatatt ccgctgaaca agaaaacgta catctggaac aaataaagaa
     5461 attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt atgaatgtaa
     5521 taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa aagtcattat
     5581 tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac aaaaggaata
     5641 aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta tttgaaataa
     5701 ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa tcagtgtgaa
     5761 atgcttgatt gaatcgatca gtgaatagtg aaatgaaccg ttcataggct tctttttctc
     5821 tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct tcaatggacg
     5881 ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg acatcccctt
     5941 gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat gtatctactt
     6001 cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat gagacatttt
     6061 caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt ggcaatcgaa
     6121 tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt acctgtttat
     6181 ttatactaac caaggcaaaa ccactcatga taccggtcac tatgattgtg agtaaaactg
     6241 tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg ataaaatcat
     6301 accgtttttc atcaaaaaaa cgtatctatc aagtgtatag gtcatgtaga ataaatctat
     6361 atactgtaga atcgaaagga gaagaagatg aacaccggag aaaaaatacg aacttaccgc
     6421 agaaattgta agcttacgca aaaagaatta ggcgaaaaaa ttggtgtttc cgataaaacg
     6481 atttctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc acttttacat
     6541 catactttag gtctgccgat tgattttcct tctattgaat cagctaccga atcaatagaa
     6601 ccaacattgc cagatgaacc tcttgttaac aagaaatctt attcatcaaa acaacccttc
     6661 tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta tctttttctt
     6721 atcaattatc aatacaaaaa tgaaacgatc gatcgattca atcctttttt gaaaccacaa
     6781 atcggctata ccacactccc tgtaggatac gtggacaatt ccatcgaacg ttgggtatgt
     6841 aatgatatct ttggccatgg tacatggctg tcttttcacg ctggagaggt agaaaaagag
     6901 aaaaaccatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg cctgctcact
     6961 tgggaagcca ttccctcaga ttaccaagaa atcctcagcc aagactatga cgacgcctta
     7021 aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag agatagaaaa
     7081 aatcaatgca catgatctta tccaaaaaac aggggtacaa attaattgaa aaagccttga
     7141 gatgaaagtt cctaaataaa aaagttcaga atgataggta tttctggctt atagtataat
     7201 atttacgcca cagcctccca atgattcttt ttggaatcat aatcaaggat ggacattcac
     7261 aaaaatattt tgctcattcg atccgaatag attaacgacc aatctagtaa gtaatataga
     7321 ttggtacgta gtcaagctgc acataattta gatcaatttt agttcttttt tatatttcta
     7381 tgatctgctc atttactttt atatgatggc ttacagtaac ataaataaat atgataatcg
     7441 ctggtagttt atccaagagt actcaataaa atacaaaatg gattgttcaa gcgcctatca
     7501 taatgataaa atgaaacaaa taaacgatac aaggaggaag agactttgag taatggcaga
     7561 acaatttttt cattgtaaaa attgttcagt tgttaatcta tgggatttcg gtagcatggg
     7621 cattttttta tagaggaatc acaccaacct ggccgtttta tctcttgtta gtgggaatat
     7681 tagtaggaaa ttttgcccct cttcactatc gtacgaactt ttttccaact gattctggcc
     7741 tttttcttaa aacaaagacg cgaaaattag aaaacattat tgaaatgaca attacctgta
     7801 taatcatttt cttagttata ttcctaaacg tcttctaata agaatctctt aaaattagct
     7861 tgttacagtt agttacctag tttatataac acagttatct tatcttcctt atcaacatct
     7921 tttgccaact ctttgtctta aattcgaaac atataagcag agctacgaac accaagactg
     7981 tttcggctct gttttttgca tcaaattcac tcttatatag ctgataatct cggctcttta
     8041 aaagaaatta ttttcgcttc attttattat aaaaaggcta ggaaccttga tgttatcagg
     8101 ttcctagcct ttatttaagt attaccccac agacccttcc atatagattt ttaaattaca
     8161 cactagttta aactaaaaca aacaaggaaa cacttaccat ttaatcataa aattagctca
     8221 tatgaatata ttagaatgta aaatagactg gtcaaaatct ggtcaaaaaa gaggactaat
     8281 acaggtagt
//
LOCUS       DACCVU010000124         8236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCVU010000124 DACCVU010000000
VERSION     DACCVU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.125"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..400)
                     /locus_tag="IVW29_002478"
     CDS             complement(<1..400)
                     /locus_tag="IVW29_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839935.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(393..794)
                     /locus_tag="IVW29_002479"
     CDS             complement(393..794)
                     /locus_tag="IVW29_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839936.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(784..1137)
                     /locus_tag="IVW29_002480"
     CDS             complement(784..1137)
                     /locus_tag="IVW29_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7839937.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1127..1438)
                     /locus_tag="IVW29_002481"
     CDS             complement(1127..1438)
                     /locus_tag="IVW29_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839938.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1435..2310)
                     /locus_tag="IVW29_002482"
     CDS             complement(1435..2310)
                     /locus_tag="IVW29_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839939.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2320..3480)
                     /locus_tag="IVW29_002483"
     CDS             complement(2320..3480)
                     /locus_tag="IVW29_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7839940.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3480..4166)
                     /locus_tag="IVW29_002484"
     CDS             complement(3480..4166)
                     /locus_tag="IVW29_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7839941.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4129..5307)
                     /locus_tag="IVW29_002485"
     CDS             complement(4129..5307)
                     /locus_tag="IVW29_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7839942.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5327..7021)
                     /locus_tag="IVW29_002486"
     CDS             complement(5327..7021)
                     /locus_tag="IVW29_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7839943.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6999..7313)
                     /locus_tag="IVW29_002487"
     CDS             complement(6999..7313)
                     /locus_tag="IVW29_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7839944.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7416..7697)
                     /locus_tag="IVW29_002488"
     CDS             complement(7416..7697)
                     /locus_tag="IVW29_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839945.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7702..8046)
                     /locus_tag="IVW29_002489"
     CDS             complement(7702..8046)
                     /locus_tag="IVW29_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7839946.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2380 a         1712 c         1177 g         2967 t
ORIGIN      
        1 tatttaattt atcgaattga cgaatcagaa gttctgtaat tggatcaagt gcaagaccta
       61 atcctcttct cataaattcg tccggctgat ttcttttaga agtacctatc cccaaatcag
      121 gatattttaa atactcaaat tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt
      181 gagtcttaag tggacgacta ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag
      241 aaacaggaat tttttctgta atcctatcca ctgcaatagc cgaacctttt gatttcaatg
      301 cttcgttaat cagtcgttcg ctctcgcttg aatagcgttc catccgcaca agaagttcat
      361 catgtccatt tatttttagc tcccaactat ttttagccat gacaatcact cttcaataat
      421 cgtctaaacg taaataccaa ttgatcgata tagcgatctt ggttctctag ttttaaatga
      481 ttgggatcca ttctctgaaa acgaatcgaa cgattttgaa tcaatgaaat aatatctagt
      541 gaatctcctg ttaaatcttc tctattttct gaatagaaag ttagatatag attttgaccc
      601 acgctatatt ttggctcagt gatcatttct atttctcctg tttcgagaat gaagtaatta
      661 aaatcatcag gtagctcatc ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc
      721 tcttctaagg aagttttgat agcagaaatt tgcttattta aacgttcttt ttctttagaa
      781 ttatcaatca ccatattcac ccacactttc aagataaaaa tagatataaa aattatcgta
      841 atcggcatag ataacgttgt aacgcatact atcgattacg ataaaatatt gatctttatt
      901 aaatttcttg gcgattggat gaaatggagt ctttactttc ttagttaatt tcgatcccat
      961 cgcatccata gctgttatat cactatctct catggaaaga tttctaaatt ttaaagaagt
     1021 gatttctgta tcttctacac caatcttttt tcctagttca tttcttttgg tagtttgcgt
     1081 caaaatcttt aaccaaccat cgttgaatgt ttcttcgagt ctacgattat tcgccattca
     1141 catcacctgc aatatattct tgtagcgcat aatgttgaat gaaacctaat aactcactag
     1201 cgaaattttg ttcaaactca tctaaagcac gattccagtc gtatctacat ctttcgatta
     1261 gcaatccgta ttctaagctt tcaggagaaa aagaaagtgt tgtactcact ttactttgaa
     1321 gataaacagc atttttagct atcatctttt taattgactc atcttcttcg ttccaggtaa
     1381 cgtaaatatt atccttcaca gctattagca attcttcagt cacttgttca ggcgtcatct
     1441 aaccaccgcc ttaattgctt taacatatgc gtaagagcat tttttcttgt ttacaaatga
     1501 taaatcttca tcaaaaggcg tagaagtcac gtatctccct ttgaaaaata aatcttcatc
     1561 gtttgttgtt actccagcat tgtgtaagat ttttacttct ttaacttttt ctattggatc
     1621 agtagcaaaa caaaagtcta attcctcgtg aactttagga ccaatattga aatacatcat
     1681 gttccaaagc tgtgcccaca tctcggctgt ccagatttgt atatttgttt tttgccctct
     1741 aaggtagcga tatagccgat tagaatccag ataaaccttt ttccaataat tcgctttagg
     1801 gcggttaata acccactgtg cgcctcctga attagtgttt atagtttcca aagattctac
     1861 tgtaacattt acaatgtttg ccatatcttt tagaatattt tctccgtttt cacagcttct
     1921 aatataatca agacttagat aactacagca gtcgctacaa taccaaacat catctttaga
     1981 aggcaatttg cgcaaattaa ttcttttatt gaaaatgaca tccgaatcga tatagaaata
     2041 tcggtcgtcc tcacgcgaat gatcttcttc taaatatttc caccataaat atggtttaat
     2101 cgaaggaata tactctttgt cgtcccgcag atcatcgtac acatgaactt caacaccata
     2161 ttccttctca aaaaaaatag gaatctgatc atcgtgtctg ctgaaaagca atacgatatc
     2221 tttgattcct agtttcttca gattagttaa acaaacttca agctcccatt taaaccgatt
     2281 gattgccggc tgacaaagaa tatacttcat tctgatcacc tacgcttgtg ttgtagttgt
     2341 tgtggttgtt ggttttgtag ttgtagtagt agttcccaaa gcgctaatat ctaatacaat
     2401 gaaactatcg ttacgtttag gttgaccgtt tgcatattgt ttagctagat aaatgcgttc
     2461 gtcttcaaca aaatggtatt catctgaagc ttcaattttt agtgtagatc ctacacccat
     2521 gaagtaatct gaggctaccc caataactgc ttttccttct ggcacagccg ttgactgcaa
     2581 atctgaaact ggtactggca atacttgtac gtattctcca ttagcagtta gtacagtctt
     2641 agctgggaat actttagacc agtaatcagt tggattcaca attaggacca catcagaagg
     2701 attcacatta cgataaatcg gatcattcac accttcgata ttgaattttg atagtcgcgc
     2761 catcaaaccg cccatagtta cagcatctaa agctgtaata ggttctgctt ttttttcagc
     2821 atattctccg ctagtttgtt tgctcatgtc acgcatcatt ccgactggca tatctttacc
     2881 agtaccatca acaattgctt gttctaatgc aattctcaat gattctacta aaacagtacg
     2941 gacataacga tctaaccata ctggacctaa atcaagcatt gccttacata caggaatata
     3001 acctgatagc ttgaactgct tcatgttaat tacatcaaag ccattatcta aaactttttt
     3061 aacagcttcg caaagtttac cccaccatgc tggattgact ccacgtgaca caatccattc
     3121 tgttacacca gttgtgttaa caaaagtaat tttttgcaat agtggatgag attgttctaa
     3181 atcttcaaat acacgttcaa atacagtagc tggcactaat tcttcgaccc ctgcaaaacc
     3241 ttcgtttttc actacttcgt tatagaattt tgtttcttgt gtagttaata cacgctgacc
     3301 acggttcatt aatactaatt gatcttgatt ttttgctgtt gcttcttcta aaattttatc
     3361 ctgaatttcc ttagataagc ttaccatagc tgcgctaaaa gattcttcgt taccatcttt
     3421 aaaagctttc atcaattggt cgcttgcagc tgttacacct tttaaatttt taactgtcat
     3481 tattttacat ctccttgtcc aaatgtttta tttaatgctg ctgtaaatgc agcaattttt
     3541 tctgctcttt tttctttaac gtcattcaaa atttcttcaa cgctttgttc ttttttagct
     3601 tcagtacctg agctattttc tgcatcgata atttcatcga ccaatccata actcaaagct
     3661 gtttctgcat ccataaacga ttctttttca agaagttctt gcaatgcttc atctgtgcca
     3721 ttgaatcgtg ttttatatga agcctttacc gatttatcaa ttgattccag ttggtcagca
     3781 atcgtacgga agtcatcgac atttccttct ccgtatgtgg aagcgcggtg aatcatcaat
     3841 tgtgcattgt tgtagatttt tatagtatcg ccagccattg cgataattga agcagcacta
     3901 gcggctaagc cgttaatcac aacgttaact tttgctttat ttgacttaag taagttccca
     3961 atagcaatcc cttgaaatac gtctccaccg tttgaattaa ttactacttc aatttcttct
     4021 tgatcaccta gactatccaa aatatttttg attcccttgt cagtattccc ttcaaagaac
     4081 caactagaac caataaatcc ctgaataaaa atttgcggta ctgcgccttc attctttact
     4141 gctagaaatg ttttcattgt cgtcattcgc ctcacctcct ttcgatactt gttgattgtt
     4201 tttagttata aatatttcat ctgccatcgc cttatcagag cgatcatttc caacgcgctc
     4261 tcttccttcg ttgattgtaa atactccatt tctaatgcct acatcaatag cgtcaaccaa
     4321 atctttgaag ctagtaatct tgatcatagt tgtatccaca cgtacaaaat tccctgacaa
     4381 gtattcttct acttcataga gactagcgtt aaacgcatcc tgaataagtt cagcaatcgg
     4441 tatgatttcg aacattaaaa aagcgtccac ttgatccgat aacccactca tgtctccctt
     4501 tagtaggttt ttcggaacgt gaaacgctgc tgctgtcatc tcaaagatgt cgtctattaa
     4561 gttttttata tctcttgaat tgctttggaa gtttccgctg aaatcttcta atgtgtactc
     4621 attttgtaat tgaaataccg cacctgcatt atcagcttcc ataaaagcct taaattgtga
     4681 tgtcatcatt ttattgattt gatcttgtgt tgtattgtct tgcggtcgga ataaattccc
     4741 tttcagtacg tatctacgag cgttagagcg cttgtaaaca ttcatggcac tagaaatgag
     4801 tttcccatac gcttgataat acgcatcgac tagttgccta atttgttgat ctgcgtattt
     4861 tatatagata acatcacttt ctagaaattc tctatcaagg actatgttgt taatttgcac
     4921 ttgagaaaac acatcatctt tcaatgcata ttctgtgaca tcccaactat ccgcaataaa
     4981 tatttcgcta gaattattag acggagaaac gatcaatact tcattgtaga atattaatct
     5041 cctgatcagt ttttttctaa attctgttgc attatttttc ttattaggag ctacattcag
     5101 cctatagtaa agatcattct ttttattttt tccatcttca tatgacttga attccgcttt
     5161 actcatcgca tttgcaatca aatcaataca agtttcaatc gcaaattttc gatacacaaa
     5221 atcaacttgc aatttacaaa agtattcttc taaaggaacc gttgcttttt ttgtgaagta
     5281 tcctaccgcc ttttgaaaaa tccccacttt ctcacctcct ttcaagttag aatactagag
     5341 gagtaaatcc agttcctgta ttttctactg agctatttgt gactgttaca ggagcagaat
     5401 cataaatatc atctaaaaaa ttcaaaccat gaaggaatga aaaaaagcca tccgtttttc
     5461 tagtttcagg ttctattttt tcatagcgta tatttccatt agaaatatgc tcttcatata
     5521 cattcatgca ataccaacgc ataatcgcat cgtcaccaaa aaataaacgt tgattaataa
     5581 aaaggtcatc aaccagatct tttaacatac catgtgtaac agatccgctt cgaacaattt
     5641 ccacagtaaa acctgcttct tctaaagcgg gcttcaatat ttttgcacgg tacatatcca
     5701 tagcgatttt tttaatataa tatttattac tcatttcaag aaaccaacct acaatataat
     5761 cagcttctat attttttcca tgaacgatct gtgattttcc ttgatctata gaaatatcta
     5821 taacctctcg tttgatgttt tgtaatcgaa gggctgattc gtggataaaa gtatgttgtg
     5881 taaaataaac atctttatcg tattttccta gcaacccaac gctggcaaaa tctcgtctat
     5941 cagcaaaatc gactgttcct atcacttcat ccattttttc aggaaattct ttttctttcg
     6001 tatgcagaac atcatcatat gaagcaacag caaatcgtgt atcttccata ggtctgttca
     6061 ttcgtttggt catgaacgta agtcttaaac cagcattacg ttgcatttga gagtattctt
     6121 gaaacatttt ccgttttaaa tctgcattgt aattaatagt tggacaagct ttttcccaca
     6181 tgtcgggatc atcaacttca ttatcgttat ccaagcgaca aataaatgga aacaaactag
     6241 aaaattctgc tccatccttg tcaattccaa gttctccaga aagaatcatt tttgattctt
     6301 ctataatgtc atcaagcgga ccaccacgaa catgaccatt agttgtatca taaaattctc
     6361 tataatctcg aattttacca ccaccagaag tagccacatt tatcattgaa taatcttcat
     6421 tttcgtgaat ttcatcaaag cggtttgcac ctggtcgctt cccatctttt gttctagcat
     6481 ttgccgtgtt ataacgaagt ttgctgtttg tagcgatatt ttgaataact tccttcgtag
     6541 ctttaaatac ttttttatct aaatcaggat gatctttaat tactttaaat acatcatcaa
     6601 aactagtctt tgcttggctt tcattattgg catagatatc aatatcataa tttttaatac
     6661 cgtgtttagc ggttagtaga aagaagttgt tccaagaagc aaaaccagtt ttaccattac
     6721 cacgtcccat taatgaaaga tatctattga acactagtgt tttatctttt ttccatcgaa
     6781 caccataaat aaaacattgt agaaattttt cccacggaat taattcgaat ggaaagtatt
     6841 gtgctggtat attgattgaa tcctctacca tctgcttatc gaagtaaata tcttctctag
     6901 taaagactct ttcttctaga taatttttta gcaataattg ctctttgcat accttgatag
     6961 tgccttcttc tatagctttg aaccaatttt caatatgctt ataactcagg aattgattca
     7021 tttgcttcac ctaccaattc aggagtaatg gcaagtttat ccaacatcaa tcccatttgt
     7081 ttgttgacag aaacaagcaa cgctactgat tcattctttt taccattctc cagtctaatg
     7141 ccgttctcgg atatatcttc ttccagtgat atcgccgttt cccataaact gatataacga
     7201 tcaacattat ctaagaatgg ctcaatattt gttttctgac tttccaattg gcttattaaa
     7261 gagcggcgta atttttctct gtagcgattt tgagacaatt cgtttttaaa cattttagcc
     7321 ctcctttcat gataaagttc gaaaaaatct cttttcctga cagccccctc cgtttcatca
     7381 cccccaaaaa atttgcgatt tattttaagg gggggttatc tcaccatcgg aatgaaagct
     7441 tcagcgaagt caatgtaata attaatctct tcaatactat atccaaaaac attttttatt
     7501 ctttcgacgt tattatcttt attcaacgct tctcttactt gattcacttt gtatttacta
     7561 caacagttat ctgataacag atccctaata cctacatagc gaacgtatat caaacgttta
     7621 attaatccct gagtataaga tgaatactct tcaatctttt ctgtatcata ctctctgcca
     7681 ttgtcattga tgatcatgca cttaccacct ttcacttgca tcgaagttag cgaagctttc
     7741 tatcttcttc tcttgtttat ctaacgctgt aagatatctg ccatgaactt cattatgatg
     7801 ttcaacacat aaacaaataa gattatctaa atctaaagct aagtcaggtc tatccttgac
     7861 ttcctttata tgatgaacgt tctctactct atgatactta cctagtcttc tacactcttg
     7921 gcattcatag tgatctcgtt tcatcgcttt ctctctaagc ctgcgccatt taggagactg
     7981 atagaactta accaaacgat cttctcttat caactgtaat aaccatctat agaattcctc
     8041 ggtcatgtcc catctccttt cgcaatctta tttaatactt agctattctt ttgccataca
     8101 atggaataac ttcattgctt tccttccgtt tatatgtatc gctctttatt ggtcttctat
     8161 actttcgtac tatctcaccg ttaccgtttt gcacagtgat tacttcatgc ttctgttcta
     8221 agtattgtgg tctata
//
LOCUS       DACCVU010000125         8054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCVU010000125 DACCVU010000000
VERSION     DACCVU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.126"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(133..306)
                     /locus_tag="IVW29_002490"
     CDS             complement(133..306)
                     /locus_tag="IVW29_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7839947.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVW29_002491"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVW29_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVW29_002492"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVW29_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7839948.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVW29_002493"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVW29_002493"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7839949.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVW29_002494"
     CDS             5704..6489
                     /locus_tag="IVW29_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7839950.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVW29_002495"
     CDS             6502..7353
                     /locus_tag="IVW29_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7839951.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVW29_002496"
     CDS             complement(7477..7929)
                     /locus_tag="IVW29_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7839952.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2708 a         1370 c         1709 g         2267 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatt
//
LOCUS       DACCVU010000126         8048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCVU010000126 DACCVU010000000
VERSION     DACCVU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.127"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            73..906
                     /gene="msrA"
                     /locus_tag="IVW29_002497"
     CDS             73..906
                     /gene="msrA"
                     /locus_tag="IVW29_002497"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7839953.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            986..1975
                     /locus_tag="IVW29_002498"
     CDS             986..1975
                     /locus_tag="IVW29_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7839954.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2264..2962
                     /locus_tag="IVW29_002499"
     CDS             2264..2962
                     /locus_tag="IVW29_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7839955.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3112..4428
                     /locus_tag="IVW29_002500"
     CDS             3112..4428
                     /locus_tag="IVW29_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839956.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4445..5860
                     /locus_tag="IVW29_002501"
     CDS             4445..5860
                     /locus_tag="IVW29_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7839957.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6324..6644
                     /locus_tag="IVW29_002502"
     CDS             6324..6644
                     /locus_tag="IVW29_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7839958.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6670..6987
                     /locus_tag="IVW29_002503"
     CDS             6670..6987
                     /locus_tag="IVW29_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7839959.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7016..>8048
                     /locus_tag="IVW29_002504"
     CDS             7016..>8048
                     /locus_tag="IVW29_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7839960.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         2649 a         1313 c         1707 g         2379 t
ORIGIN      
        1 cttatttcct cactttatga taaattacag ctaaagtcac tagcttgtct tcttcggaaa
       61 ggaggagaat ctatggatga ggaacaaata ttcattcaaa cactttataa tctgattttg
      121 aatccgaata cacgagattg ggaacgaaaa gtattgattc agacaaagaa tgacataaga
      181 gaaaatatat ccgttaaaga acagttatca aaactagaag caacactacg tccattagct
      241 atccgaatga atctgacgcc tgatgtaatg gacttttatt tattgattac ggaaggattt
      301 gataaggagc aaaaatatga cttttccaag catgccatgc aagatgctga ctatcaagaa
      361 cgtgcagtat ttgcaggggg atgtttctgg tgtatggtcg agccgtttga atcgaaaaaa
      421 gggatcctat ctgttttgtc aggatacacg ggcggacatg ttgaaaaacc aaattatgat
      481 caagtaagtg gtggttacac aggtcatgtg gaagctgtgg agatcattta tgacacaaga
      541 gaaataagtt atagcgagtt gctgacaatc tattggcaaa ttactgatcc cacagataca
      601 tttggacaat ttcaagatag agggaagcaa tatcgtccag tgatttttta tcaagtcgaa
      661 cgacaaaaag aattagcgga acaatcaaaa cagaaactag acagttcagg aactttccac
      721 caaccaatcg tcacaaaaat cgaaccagcc ggtacgtttt ggccagcaga aaactatcat
      781 cagcaatttt acaaaaaaca accgaaacgt tataaaaaaa tccagcaagc acgtaatcaa
      841 tttctgatct atcaacgagt aaaaaacaaa tggcaaaaaa atatacgaaa aaatcatttt
      901 gattaactcc ttttctttaa aatgtagtaa actaaaaata gaaaaaagaa agcgttttaa
      961 aaagatttga tgaggaggag aaaaattgta tattgcagat agtgcacgtt atgaaaaaat
     1021 ggaatatcgt aaatccggtc attcaggttt gaaattaccg attttatctc tcggattatg
     1081 gcaaaatttt ggtgattatg atcctatcca taatcaaaga gagattttaa gaggcgcatt
     1141 cgacatggga attacccatt tcgaccttgc gaataattat ggaggccctg ctggagctgc
     1201 agagaaaaac tttgggcgta tttttagaga agattttcaa gcctaccgtg atgaactgat
     1261 tatttctagc aaagcaggtt atcatatgtg ggaagggcca tatggggaat ggggctctcg
     1321 taaatccatc atttcaagtt gtgaccaaag tctccaaaga atggggctag attatgttga
     1381 catcttctat catcatcgcc cagaccctga tacacctttg gaagaaacag cagaagcttt
     1441 gatgcaactt gtaagacaag gaaaagcatt atatattggt atatccaact ataatggaga
     1501 agatacaaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca ttattcatca
     1561 gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc cagtattaga
     1621 agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc tgacaaatcg
     1681 ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac cattcctttc
     1741 agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa tcgccgtatc
     1801 acgaggacaa tcattagcgc aaatggccct tgcatggaat ttgcgacaaa aatctgttac
     1861 aagtgtactg gtcggagcaa gtcggttaag tcagttacaa gaaagtgttc gaatgatgga
     1921 taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag aatagaaacg
     1981 atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc aagaatcgaa
     2041 tgaagatcgt ctgttattga acatcatatt ttttgtcgtt caaaataaaa atgatcgaag
     2101 gctaggagtg aattttatcc tagtcttttt tatggtaaat atcttcttcg tttgtatgtc
     2161 tatgaacgga ttaatcaatt ttgtatacaa aaaatcatca gatgattcaa acgtgttata
     2221 ataattacga aaaaatacat cagatgaaaa ggaggtatgt ggtttggaag aaaataaaac
     2281 cctagtagaa gtaactactg acaagatcct tagatattta aaggaaatgc agttgcaagt
     2341 aggagataaa ctgccgaatg aatatgaatt gtccgattat ttagaggtgg ggcgaagcac
     2401 ggtgcgagaa gctgtgcggg ctctagcttc caggaatatc cttgaagttc ggcaaggagc
     2461 ggggacattt gtcagtgata agcgagggat tgccaatgac ccactcggtt tttctttgat
     2521 tgaagatcag gatcggatga tacgagactt atttgaactg agatatttat tggagcctaa
     2581 gatggcaggt ttggcagcgg aatatgcaac gaagaatcaa atcgtccaaa tgaaaaaatt
     2641 aacgcaaaag attgaaactt cttttcgaga aggcacacaa gaacatgttc aattagacat
     2701 cgattttcat acgatgattg cagaagctag cggaaacgta gccttgctgc atattatccc
     2761 aatcattaat gaatcaattg ctttgtttaa taaaaattat gatatccagc atctgaaaat
     2821 cgagacgatc gatattcata cagagatcgt tcaggccatc gaaaggcgga atgcacttgc
     2881 tgcgtttgat gcaatgactg tccatatggc aataaatcgg agagaattac agaaaataat
     2941 aggagaaaaa gaaactaatt gacagttcat ctgatgtatt ttatgataaa tacatcagat
     3001 gaatttttgt tttatatttg gtaaaaaaga ggagtaaata gtttctgttc atcgaaatgt
     3061 aagcgctgac taatagacaa aaagagatga cgtcaaggag aagaagcagg aatgagggga
     3121 tctggaatga gttttatgga taaattagcc aatgttttag ggaacgtggc aacgagaatc
     3181 aataatttaa ggtatatcat ggtgataaaa aacgcatttg ctgcattgat tccggtaatt
     3241 attacgggtg cttttggcac attgttttca gcaatggtct ttgactcgga aaatggctta
     3301 gcacagataa gtttcttgcg ttttttagaa gaattaaagc ctatcgcatc tgccatcagc
     3361 tatgtcacac taagtttctt gaccatctat gctgtttttc tgatagggat cgaattagcc
     3421 aagctgaata aagtggatgg tgtattccct ggtatcatcg cagtgatgtc ttatttatct
     3481 gttaatccaa ctgtttatga attagtgaat gaaaatcaga ctgttatagt agaaaacgta
     3541 ttggctaaac aatatacaga tacgaaaggg ttatttttgg gaatgttggt ctccatcctt
     3601 tctattgaac tttattgctg gttgaggaaa cagaagaaac tacagctgag aatgcctgat
     3661 accgtgccaa cgaatgtttc tgctagtttt tctgctttat tcccgacgat tctgactgta
     3721 tcgggtatcg cggctgcggg atttatcatc aaagaactta caggaatgta cctttatgat
     3781 atcatttaca atgtcgtaca acgcccatta gaaggtgtcg tacaagggtt accgggtatt
     3841 ttgctattga tgtttattgc gcaagtcttt tgggtgattg gtatccatgg aaatcaaatg
     3901 gtgaagccga tacgggaacc gttgctttta gcggcaattg ctgtaaatac ggaagctttt
     3961 gaagctggaa aagaagtacc gaacattatt actatgccat tttgggatat gtatatgagt
     4021 atgggcgggt ctggagtcac gatcggttta ctgattgcag tattgttagt tggaaaacgg
     4081 gaagatatga aacaaatcac caagttatcc cttgctcctg ggatcttcaa catcaatgaa
     4141 ccagttattt ttggtatgcc gatcatgctg aatccaattt tagctattcc gtttatctta
     4201 acgcctctta tcactggaac gattggttat tttgcaaccg ctttaggatt tgcggcgaaa
     4261 gcagttgtaa tggtcccttg gccaatgccg ccgatcgtca atgcttatct ggcaacagca
     4321 ggaaatatcg gtgcagttat tacgcaactt gtttgtatta ttgtttctat tttgatctat
     4381 ctgccatttg taaaagtagc aaatcgtgtg gaaaatgatg ggatctaaaa aacaggagga
     4441 aatcatgaaa attactattc caaaaaattt tattcttggt gctgcatctt ctgcttggca
     4501 aacggaaggt tggaaaggaa aaaaagaagg acaggattcg tttatagata gctggtataa
     4561 aaatgaacat tttgtttggc ataatgggta tggcccagcc gtagcaacta attttatgga
     4621 acaataccag caggatgtgg atctgatgaa atccatccat ctgacacatt atcggacttc
     4681 tatcaattgg gctcgttttt ttactgatta tgagaactgt atagtagatg aagaatatgc
     4741 tcagcatata agtgaaatga tcgactcttt gatcgaagca gacgtagaac cgatgatttg
     4801 tttggaacat tatgaagtcc cagcttttct tttggaaaaa tatgatggct ggtcttcaag
     4861 aaaagtagtt gatttttatg ctaagtatgc tcaaattgcc tttgatcgtt acggtgatag
     4921 ggtaaaaaac tggtttacat tcaacgaacc aatcgttccc caaacgcgtt gctatttgga
     4981 tgctgttcgc tggccgcatg aacaaaacac aaaaaagtgg atgatgtgga attatcataa
     5041 agttttagca acggctaaag cagtagagat ttaccgcagc ggaaattatg gtggaaggat
     5101 tggctgtatt ttaaatcctg aaatggtcta tgctcgatcc aattctccag aagaccaaaa
     5161 agctgcttat atgtatgatt tgttttataa tcgagtattt tttgatccga tggtaaaagg
     5221 atactacaca gaagagttcc tggagttgtg tcaaaaacaa tcgatttatt tcgatcctgc
     5281 aacagaggat ttagaaacga ttcagcatca tacagtagat ttccttggaa tcaatcaata
     5341 ttatcctaaa cgcgttcgcg cccctcgtta tcaatggaat gaagagacac ctttccatcc
     5401 tgaacgatat tatgaagtct ttgatttacc tgggaagaaa atgaataatt ccaggggatg
     5461 ggaaatctat ccgaaaatca tgtatgatat agccatgtat ctaaaagaaa attatcataa
     5521 tatcccttgg ctgataacag aaaacggtat gggaagagaa tatgaagaac agtatatgga
     5581 tgagaaagga atcgtgcaag atgattaccg tatcgaattt atcggacagc atttgcatta
     5641 cttgatcaaa gcaacagaag aaggcgcaaa ttgcgaaggc tatatgttat gggcattcac
     5701 tgactgtgtg tcaccaatga atgcatttaa gaatcgatat ggattagttc gaatcgaatt
     5761 gaacgatgaa cgaagcagaa cattgaaaaa atctgcttcg tggtatcaga ctttgattga
     5821 taagagagaa ttggatttgc ctgacgaaat ctataaataa atagaagttt tttgtagatg
     5881 ttcattgtat ttactggtta tttgaaataa aagaggttgt gaatccagcg ttttgagcta
     5941 atacccaggg tcagctgatt gaacaccgtt tattgagagg ttgtgaaaaa gctgtcctgc
     6001 atcaagtaat aagaatagca aagtaaaaac aacatcttat gtttttaact tggttggact
     6061 tattaccgca gatgcaagct tttgaatacc gtttatttga aaaaagttgt gacaagtttt
     6121 gtgtcacaac tttttttgtg cacgttaaaa gaaaaaaggt aataaaaatt aatttgtgtg
     6181 tttagtgtgc gctttctttt ttagtgtgta tgcaacttgg tatagacctt aaatgtgatg
     6241 atatcaactt tttaaagcgc ttttaaagtt ggcacgctac ttgcttatat acaagtgtaa
     6301 gtatagaaag gaacgtgttt aaaatggaaa agaaaacaat catgcttgta tgttcagctg
     6361 gaatgagtac aagtctccta gtcacaaaaa tgcaaaaggc agcagaagca aaaggaatgg
     6421 aagcagacat ctttgctgta tctgcttcag atgcggataa tcaactagaa tctaaaaatg
     6481 tagacgtttt acttttagga ccgcaagtac gctttatgca aaatcaattt gctgaaaagc
     6541 tagcacctaa aggcattcct ttagatgtaa tcaatatggc tgactatggc atgatgaatg
     6601 gtgaaaaagt actcgaccaa gcagaaaaac taataaacaa ataataaaaa ttatcggagg
     6661 atacaataaa tggaagatca aaaaaatcta gaagccatca tgggtctgat catgtttggc
     6721 ggaaatgcaa aaagcgatgc aatggaagcg attgctgctg cgaaaaaagg tgatttcact
     6781 ttagcagatg aaaaaatcaa agatgcagag gaatctttag tccaagcaca tcactctcaa
     6841 acagagatgc tgacacaaga agcacaagga aatcacgttg aagtcacgct tttgacggtc
     6901 cacagtcaag atcatctgat gacatctata gcttttacag atttagcaaa agagatcatt
     6961 gatctgtatc gtcgattgaa cgcgtaatct acagtaaaga aaagagggaa gatttatgga
     7021 taaatttgtt gcatttatgg agaaacattt tatacctgta gcatcaaaaa ttggtgcaca
     7081 acggcattta gttgcgattc gggactcatt tatggtgagt atgccgttaa tgatcttagg
     7141 agcattagca gtaatgatca ataacttacc gattcccggg ttccaagaat tgatgaactc
     7201 aatttttggt ggtgaatcat ggaaaggatt cggggctgct gcttggaacg gtacatttgc
     7261 cattctttct gtcttgattg ctttcttgct tgcttatcat ttagctaacg gctaccgaaa
     7321 agacggtgtt tccgcaggtg tgatttcttt aggctctttc ttcgctttag gcggagcatt
     7381 aggtatgagc tcaaacggtt tgtttattgc tattattgtt gggattattt caacagaaat
     7441 ctttgtacgt ttaagcggaa atgaaaaatt gatcatcaaa atgcctgatg gtgtgccacc
     7501 ggcagttgca aaagcatttg cttcattact accagcaatg atcactatct ctgcgtttgc
     7561 tttagttgct gcaatttttg ctgcattcgg tgttgatgat atcgtaggtt ctttctatac
     7621 agtggtccaa gaaccattta tgggacttgc aaactcttat ccatctgcct tactgcttgc
     7681 atttatcaca ccattcttat ggttctttgg tttacacgga gcgaatatgg tcgatccttt
     7741 gatgcaaaca atcaatgcgc cagccattga agcaaacgtt aatgcaatcg cagctggaca
     7801 tagcgcaccg tttatcgtga acaaaccttt ctttgacagc tttgtcaact taggaggaac
     7861 aggtgcaact ttaggtttac tgttagctat ttatttagta ggacgtaaaa acaaaccata
     7921 tatggttgta acaaacttgt cgattgcgcc aggtgtcttc aatattaatg aaccaacgat
     7981 gtttgggtta cctatcgtgt tgaacccgat catgttcatt ccatttattt tgacaccaat
     8041 ggtcttag
//
LOCUS       DACCVU010000127         8004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCVU010000127 DACCVU010000000
VERSION     DACCVU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.128"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..429)
                     /locus_tag="IVW29_002505"
     CDS             complement(<1..429)
                     /locus_tag="IVW29_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839961.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKK"
     gene            597..914
                     /locus_tag="IVW29_002506"
     CDS             597..914
                     /locus_tag="IVW29_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839962.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            847..1326
                     /locus_tag="IVW29_002507"
     CDS             847..1326
                     /locus_tag="IVW29_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP7839963.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1776..2207
                     /locus_tag="IVW29_002508"
     CDS             1776..2207
                     /locus_tag="IVW29_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839964.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2204..2530
                     /locus_tag="IVW29_002509"
     CDS             2204..2530
                     /locus_tag="IVW29_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7839965.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            2649..3335
                     /locus_tag="IVW29_002510"
     CDS             2649..3335
                     /locus_tag="IVW29_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7839966.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3310..4659
                     /locus_tag="IVW29_002511"
     CDS             3310..4659
                     /locus_tag="IVW29_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7839967.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4650..5123
                     /locus_tag="IVW29_002512"
     CDS             4650..5123
                     /locus_tag="IVW29_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7839968.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5125..5625
                     /locus_tag="IVW29_002513"
     CDS             5125..5625
                     /locus_tag="IVW29_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAP7839969.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5640..7952
                     /locus_tag="IVW29_002514"
     CDS             5640..7952
                     /locus_tag="IVW29_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7839970.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         2834 a         1357 c         1773 g         2040 t
ORIGIN      
        1 ttttttttga taattagctg ttcttatgac cattacaagt ttttcaccag gtaatatatc
       61 tttcatatct aaaacttctg aaaaagcgac tgagtagcca ggagagattg cttgtgccaa
      121 cggtatagga gataaaaggc tacttttatg aatgcttccc atatcaaaac cattggcgtg
      181 ataatgtgaa caattaattt tcttaccgtt gtaaagatac gtacattcga taattgagtt
      241 tggcattttt gatgggtttg caattacgcc agtaagaatc attcgattac ttctatcatg
      301 cttaactaaa aaataggaga gcgtaatttt tcttttatct ttaaataagt tatagaggga
      361 taaaaaaaat cctgaaatag aaatgattaa agtaatagcg acttggtaat cgctcaaaat
      421 ttttatcaag taattcacca cctttttact caaattatat caaaaaggag agaagaaata
      481 atgcaagaat tagtaatttt gaaaaataaa aaaagacacg aacgccggca agcatgatcg
      541 tgtcagggaa ataaactatc tttctatatt ttaccacaga aaggaatgaa ccgtaaatgc
      601 taattgcaac ggatacacta gaccgcatct ttttaaaaga cgaatacaaa ctgcgcaata
      661 tagatgcgtc aggaatttta gtatttgatc tttatgacaa tggaaaaatt ggtatctatc
      721 aagcaagtga tattgaagaa acaaaccttg ctttcgaaca aattgatgat tctgtggaat
      781 tggatttaga tgaggcaatc ctagcttttg aacaaattgc aaaattatta aaggaggcac
      841 aaaagaatgg caactcttta ccaactcagc gagtcatata tcaaagtcct ggaactggca
      901 gaacaattgg atgaagaaat tcttcgcgat actctcgatt cgattaatga agcgatcgag
      961 tataaggcag aaaacttagc aaaaatagtt aaagaagtag aagggaaagc tgagttaatc
     1021 gatagtgaaa ttaaacgtct gcaggaacgt aagacatcgc ttttgaacaa tgctaagagt
     1081 atcaagcact atttacaaga ggaaatggaa aagactggta aaacgaagat taaaggtgaa
     1141 ttattcaaca ttggaattca aaataatcca gtatcggtca atgtaatcaa tgagaactta
     1201 attccaaaag gattctttac ccctgtgcct cccaaattgg ataaaaagca attgaaggag
     1261 gaactgaagc acggagatat tcctggtgct gaactcgtcc aaacaaaagg tttgaggata
     1321 agatgatgaa ggaggagaaa taatggaaca cgaaaacgta aaagacgctt tgaaagctgc
     1381 tatcgaaatc gcggaagcta aaggaataaa ggttgacggc aaaccagcaa ctgtccatga
     1441 cattcaaaat ttgacgaaag aacatcttta ttttatcgcc gacttgcttg gtttgtcgga
     1501 attgtattta gacaaataac cccacacgga aggagtggct agggtggttg tattacaagt
     1561 aatagtctgc gtaacattca tttggtcgtg cataccggtt tgtatatata tcatgaagaa
     1621 gcttcgccct gagtcttaca aattatacgc aatttacagc ataacagtag ctcttgcatt
     1681 tacattgttt gcaatttcta ggcttccgtt ttaatcgtgg gaatgaattt ttaatgaact
     1741 aaattatcta taaagcaaaa aaaggagaaa caacattgaa aaacaaacta gcaaaaacaa
     1801 cagcaatcat cggactagca ctaggtagcg gcgttatcgg ctacgcagca agcaatgcgt
     1861 ttcaagattt ggacacgatc aaagcaaatt tcaacacagt cctacaatac ggtcaaacga
     1921 aatcgcaacg tgtgtcagaa cttgaatcac agctatccaa caacactcgc acacaggagc
     1981 agctgaaagc cgaaattgag caaatcaaat cggacaagca gaaggaaatt gaagccaagc
     2041 aacgagaagt cgaacaaaag caacaggaga tcgctacaaa gcaacaggaa gccgatagct
     2101 tgcgccaaca actatccaca gtgcaaaacg acaaggagca gttagaacag cgcgtgagcg
     2161 agttacggca gtatacggat caaaaggtag gggagttggg gaaatgaaag agttgactat
     2221 ttatgaccca aatatttcat gggctaaaca cacaattaaa gtttctttta tgatttgggg
     2281 ttacaaagga tatgttactt acaaagtggg cggtaacact aagggtttat cattaatagc
     2341 tattgattca gatgatctat atgatgctaa tttcgaggat aacccagtta atttccgtga
     2401 ccttgatgaa gattggttct ctatggaact tactaacgat aaaggcgaca gtacacttgt
     2461 agaagatgaa ttcgacagac taggtgatta tattgtcggt gttgaaatta ttgcgcacga
     2521 accagagtag gagagtgaat acaaaatgat taaaagaatc actatatcag ttgaagaaga
     2581 tagtggatca acaatgcagc ataccgacaa tcgaaaaact tttttaacca atagaaagga
     2641 gaattttgat gcagattaag aaggcgacgg atatcgaaca aaccaaggga acatatttga
     2701 tctacggtgc tccaggaaaa ggtaaaactt caacggctaa atattttcca gggaaaacac
     2761 tgatccttga tattgatcgg acttcaaaag tccttagagg tgagaaaaat gtcgatatcg
     2821 tatatatcga caacgaagat acctggaacg actggggcaa cacgttggct gatttaacta
     2881 ccaattatgt aggtgtttat gacaacgtgg ttgtcgataa tgtcagcgaa ttggaacggt
     2941 gtattctgtc cagtttaggc gcagaaggaa aaaacaacgg cgttccttcg caaggcgatt
     3001 atcaatatat gcaatttaga atggtgaaca gcctacgcta tatgaaaaat ttagacagca
     3061 accttatctg gaccgcatgg gaagaaatcg atttgtggac ggattcggat ggatcttctt
     3121 acaacatcgc tttgccgcag atcaatcgaa agattcgaaa caatattttg ggattatgtg
     3181 acgttgttgg tcgattgatg gtaaaggaag acgatgaacg cggatttatt ttacaagcca
     3241 cagattcaac ctatgccaaa aatcaattgg ataatagatc tggctgtaag caaagtgagc
     3301 tgattttaga tggaattgta cgactaccag aatgatttag tcgatcgtgc aagacaagcc
     3361 tatgttgatg gttacaaggc accatgtatc gtatcaccgt gcggttctgg aaaatcagtc
     3421 atggttgcag agatcgctaa acgaaccaca cgaaaaggca acagagtgtt gttcttggtc
     3481 catcgtcgtg aattgattga tcagatcaaa gatacgtttc aaaagatggg cgtagataat
     3541 cagcttgtaa atttcggcat ggttcaaaca gtcgtacgac atcttaaaac catcaagaaa
     3601 ccacaactga tcatcactga cgaaaaccat cacgggttag cagcgtccta cagaaaaatc
     3661 tatgagtatt attcggacgt tccgcgatta ggttttaccg cgacacccat ccggttaaac
     3721 ggtagtgggt tgggggatgt gaacgacttg ctaattgaag gagtatcggc taaatggttg
     3781 attgagaatc atagattagc tccttatgaa tattatgcac ctaaattgat cgatacagcc
     3841 gaattaaaga aagcttcgac tggagatttt acaaagaaat caatggataa ggcagtgaag
     3901 aatactattt atggcgacgt gttgaaacat tataaaacgt tagcagaagg tgaacaggca
     3961 atagcatact gtcacagtat cgatgcaagt aaacacactg ctgaaatatt caacgatgct
     4021 ggttataaag caacacatat cgatgcaaaa actccaaaag atgaacgtgc agatattatc
     4081 gaagcgttca gaaatcatga aattaaaatc ctatgcaatg ttgatttgat cggagaaggg
     4141 tttgatgttc ctgattgttc gactgtaatt atgttgagac ctactcaatc gttatcattg
     4201 tacattcagc aatcgatgag ggggatgcgt taccgaccag ataaaacgtc aatcatcatt
     4261 gaccatgtgg gtaatgtgaa ccgttttgga ttgcctgata tggatcgtga atggagttta
     4321 gatacaaaaa agaaaacaaa aactgatagt gatttatcta tcgttcaatg cgcgttttgt
     4381 tttggtgctt acgaacggcc aaaaggcgat aatatttgtc cgtattgcgg agagttgcag
     4441 ccgattgaag aacgaaagtc agagttagaa atcgatgaat cggctgaatt aatgaaagtc
     4501 ggagaaacga aaattacttt gaattttgag aacaataaat attacaacat gacggaagat
     4561 gaagcagaaa gtatagagga cttatatgcc atagcaagag ctaaaggatt taaacctggt
     4621 tgggcttata tggctgctaa gaggaagggg tggttgtagg aatgggcggt atttttggta
     4681 aaaaagcaga agatgttcaa ggttcaataa taaatggcgt aacaataata ggtgatactg
     4741 gaaaaagaga tagttcaggc aatcaaattg taatcgctag aaataaaaat aacgaagttt
     4801 ttgaatcttc tctatctaat ataaaaagag gtgttgttaa cgggacaggc gaagagagtg
     4861 gctacgttaa tttaaaaaaa gctaatgaaa aataccatgt tgaaggaact tacgttaagg
     4921 ctttgtctgt cggtggaaca aaaggcgtta attgggataa acgaagaaat aaatggaaag
     4981 cacgattata tttcaaagga gaatatgtct tacaaaagga tttcaacacc aaagaagaag
     5041 caatccaagc acgacttgca gcagaagaaa aatatttcaa acctatctta gaaaaatatg
     5101 aaaataaaaa ggagaatgat taacatgaca ggatttaact tagacttttc aaaagcgcaa
     5161 caagggaacg aaatcaaaga cggtacgtac gaagtggtcg taaacaaagc tgtagaaaat
     5221 gcaactaaat caggcgcaga atttatcgat atcgatttga tcgttcgtaa cgatgtggat
     5281 caaccgttcc aaaataaaca cattttcgca aaaatctgga aagccaaagc taccggcaaa
     5341 tataacgaag gtatgatcat ggcaattgca caagcgctac agttggaaga cggaaaatca
     5401 tataacgggt ttgatgaatt gctggcggac tttgttttga aaaccgcctc agtacgcgtg
     5461 aaaactgaag agtcaaatgg atataaaaac gtcaatgtga agtcttggga taaaacgaac
     5521 acacggggcg taatgaatca ccaattcaaa aatggtgatg aaccatcctt cggaccggaa
     5581 cgttcaagag cgacaacggt tagaaatgac aatcttccat tttaattgag gtgtaatcaa
     5641 tgtatgaaaa aattccaaaa gagcttaaaa atctcaaaca atggtgtgtt tataagctag
     5701 tttgggatga aaagcggcag aagcacacga agatccctta taacgccaac aacgggcata
     5761 aagccaaatc caatgacgag agtacctggt ctgattttca gaccgctctc gctgctattg
     5821 atcactacgg aatgagtgga ctggggttct ttttcaaacc accttatttc ggcattgata
     5881 tcgataatgc tgaaggtgaa gttgagcgat ttaaatcagg tgatatagaa gaaaatatta
     5941 tctatgaatt tatcgaaagc atgagatcct atgctgagta ttctcaatca ggtactggca
     6001 tccacattat tgctcgcgga gaattgcccg gcggaagacg caggaaaggt gacgtagaaa
     6061 tgtaccagaa cggccgattc ttcgttatga ctggaaatgc cgcatcaaaa tatttagaaa
     6121 taactgagcc gaatccaaag gatatcaaac gcctatatga tcgctacgtt ggagatgaaa
     6181 aaatcatcca attcaaagaa gaaaatccgc tgatgaatac cgttgattta ccggttgaag
     6241 aaattatcaa acgtgctgaa tcgtcttccc aaggggcaag attcaaagtc tttatgaacg
     6301 gcggttggga agctttttat tcctcgcaat ccgaagcgga tatggcgttc gccaacgact
     6361 tggctttttg gaccggtaga gactttgaaa aaatggatga gattttcaga gcgtctgcaa
     6421 tgatccgtag caaatatgac caaaaaaggg gatcggcaac ttacggagaa tctctactaa
     6481 ataaggcaat tgctgataca aattccgtct acaaccccaa acggaaatct gattttaaga
     6541 tttttatcaa agaccaggaa cagccaaaag aggaaaaata ctattcgtac gacgacaccg
     6601 gcaacgcgga tcggtttact gatatctacg gaacgttagt caaatattcg tacattgata
     6661 aatcttggta ttactacgat gggaaagttt ggctacagga caataccggc gaagttcgga
     6721 aaatgatcga tacgacagta gacatcatgg gaaaagaacc gttaacgatc cctgaaggcg
     6781 cagacgatga aaccaaagaa gctttgatga aagcgaaaga aaaacacgtc aagcgttctc
     6841 gcagcaatgc cggaaaaaac gctatgatgg acgaattgaa acatcgacta tccgtattgc
     6901 cggaagagtt cgacaaagat aaaacgctgt tcaacacaca aagcggctat ctgtctttgc
     6961 atgacggtct tctacacgag catgagatag acaaaatgtt tacacgtgtt tcaaatgtcg
     7021 agtacacaga atcggtcgat tgtcctatgt gggaagaatt tattaatcag atttttgaca
     7081 atgatcaaga attaatcaga tatatccaga aatgtgtcgg atattccctg accggatcta
     7141 ccagagagca atgtatgttc atcctctacg gtcatgggtc aaatggaaag tcggtatttt
     7201 tggaaattat ttctgaattg atgggaaact atgcgatgac tatgcaggca caaacgatca
     7261 tggtcaagca aagccaatca tcagcaaaca gtgatattgc acgattgaaa ggtgcgcggt
     7321 tggtcacttc gtcagaaccg aatgaaggcg tccgactgga cgaaggtctg gtcaaacaat
     7381 tgactggtgg tgataaagtc acggctcggc acctttatgg aaaagaattc gagttcgaac
     7441 ctgaattcaa gttgtggctg gcaacgaacc acaagcccat catccgtggg accgatgacg
     7501 gcatctggcg acggttaaat ttaattccat tcactgtcca aatcccggat cacaaaaagg
     7561 ataaaaatct gaaatttaaa ttgcagacgg agttgcaagg gatccttaaa tgggcaatcg
     7621 atggttgcct gttgtggcag agagagggct tggagaaacc aaagtcggtt gttgcagcga
     7681 gtcaggatta tcgaaacgaa atggatcaaa taggtacatt tatcgaaacg tgttgcgaaa
     7741 caggtccagg actaaaaata tcaggcggag aattatacaa agtttatcgt gaatgggcat
     7801 cagataatgg cgagcatact ttcactaaca cgaagtttgg cagagagatt tcaaagaaat
     7861 atagcaaaga aaaagctggt ggatttatgg tttataaagg aatcacatta aaaccaagaa
     7921 aatacgataa cgttagagaa ctttttaaat aaatctggag ggttctggag ggttgagaac
     7981 gaaaccctcc aacccttaga aacg
//
LOCUS       DACCVU010000128         7998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCVU010000128 DACCVU010000000
VERSION     DACCVU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.129"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVW29_002515"
     CDS             complement(<1..132)
                     /locus_tag="IVW29_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7839971.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..2914
                     /locus_tag="IVW29_002516"
     CDS             542..2914
                     /locus_tag="IVW29_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7839972.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2975..4435
                     /locus_tag="IVW29_002517"
     CDS             2975..4435
                     /locus_tag="IVW29_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7839973.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            4446..6650
                     /gene="topB"
                     /locus_tag="IVW29_002518"
     CDS             4446..6650
                     /gene="topB"
                     /locus_tag="IVW29_002518"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7839974.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6891..7484
                     /locus_tag="IVW29_002519"
     CDS             6891..7484
                     /locus_tag="IVW29_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7839975.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            7497..>7998
                     /locus_tag="IVW29_002520"
     CDS             7497..>7998
                     /locus_tag="IVW29_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7839976.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPT"
BASE COUNT         3066 a         1324 c         1686 g         1922 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
     1081 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
     1141 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
     1201 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
     1261 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
     1321 tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata aattctattt
     1381 tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt
     1441 caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgaaaa
     1501 ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga
     1561 aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta agcaagaagt
     1621 caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgaaga
     1681 cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta gaaatcaatt
     1741 attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg cgttgaaaaa
     1801 agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt
     1861 caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga
     1921 taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga
     1981 aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc
     2041 gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat
     2101 tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta
     2161 tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta ctggcgaaga
     2221 agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa gtattcatga
     2281 attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca
     2341 ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt cggagaaagc
     2401 tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagcggc
     2461 ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat
     2521 ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa
     2581 gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc ccgttggttc
     2641 actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca
     2701 tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt taaaaggaaa
     2761 aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc taggaaatcc
     2821 attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg aaatctatta
     2881 tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac tatctgaaaa
     2941 acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa tgagtcttat
     3001 tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtagt
     3061 caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg atcgaagaaa
     3121 acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat tagctaaact
     3181 ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag ccacagattt
     3241 tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg agacagaaaa
     3301 gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga attatctgaa
     3361 ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag ggttgattgc
     3421 cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc gtttagatca
     3481 tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg cgagttatca
     3541 aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc atatagataa
     3601 aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc agttaaaatt
     3661 ttttgagagt agtattgatt tacttagcta tgctgcacta aatcgtgatc agttaaacga
     3721 tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt attttggtga
     3781 ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga tttgtgtgga
     3841 taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg cagttgatcc
     3901 atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc aagtgccaaa
     3961 agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac cagaagctat
     4021 tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg tttccgctca
     4081 caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag aagctattaa
     4141 tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac gcgtggatgg
     4201 tacgtacgat tttgatcgtt tttatcaaga aaaaggggat attaatgctc agattctttt
     4261 ctcttataaa gcagaacaat actacaaagg ctataaaaat catgaacatg aatttgtacc
     4321 tgaagtaaaa aaggattgga atgatcaatt gaagcatgaa atctaccaac aagagatcag
     4381 aaagcaaaaa cgtgccatgt tgtttcaaca aggtagacag caagaaaggg agtgaaatcg
     4441 tcatgatgaa aaccgttatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata
     4501 gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga ttgttttcag
     4561 gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt
     4621 acgatgaaaa ttggaaacaa tggtcattag agcatttgcc tattttccct acacattatc
     4681 actatcatgt tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag caacttcaat
     4741 cggccgacac cattattatt gcgacagata gcgatcgtga aggggaactg atagcttgga
     4801 cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt tggatcaact
     4861 ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca gaagagacct
     4921 acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc ggcatgaacg
     4981 gtagcccgct gtatagttta ctcttgcagc aaaaaggcat accaggaagt ttttccttag
     5041 gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag attagaaact
     5101 tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg
     5161 atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa gattatttaa
     5221 aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt gaaaccgaga
     5281 aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct aaaatgaatc
     5341 agctcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg tatgaaggga
     5401 aatatctaag ctacccacga acagataccc cttatattac agaaggggag tatgcgtatc
     5461 tgttggatca cttagacgaa tacaaacact ttttaaaggc cgaagccatt cctactccta
     5521 ttcatacacc gaatagtcga tatgtgaata ataagaaagt ccaggaacat tacgcgatca
     5581 ttcctacaaa gacggtcatg acggcagcgg cttttgaaca attaagtcca ctgcagcagg
     5641 ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag tatacctatg
     5701 aagaaacaac gatcttgacg caagttcagc agcttcagct aaagacagtc ggaaaagtac
     5761 cagtagatct aggttggaaa aagctgtttg gcaaagagag cgacagaaaa gaaaaggaag
     5821 aagaaccgct acttcctaaa gtgacaaaag gagaagcggt gacggtagat ctacaagtcc
     5881 ttgaaaaaga aacgaagcca ccacaacctt ataccgaagg tacgttgatc acagcgatga
     5941 aaacagctgg aaaaaccgtg gatagcgaag aagcacaatc catattgaaa gaagtagaag
     6001 gaatcggtac agaagcaaca cgagcaaata ttattgaaac tttgaaacaa aaggaatata
     6061 tcaaggtaga aaaaaataag cttgtggtga caaataaagg gattttactt tgccaagccg
     6121 tggaaaaaga gccgctgcta acaagtgccg aaatgaccgc caaatgggaa agttacttat
     6181 taaagattgg tgagcagaaa ggaacacaag ctactttctt agctaatatc caaaaatttg
     6241 tctctcactt gttggaagtc gtaccgggac aaatccagtc aacagatttt ggatcaacgt
     6301 tacaagaggt gaaagcagcc agtgaaaaac aggaagcaac gcgtcattta ggtgtttgtc
     6361 caaaatgtcg ggaacaagaa gtcttgcttt atcaaaatgt ggcggcatgt acgtctgaag
     6421 cctgtgattt taaattatgg acaacgattg ccaaaaagaa actcacagcc acacaactga
     6481 aagagatcat tcaaaacggc cgaacaagcc aatcagtcag aggactaaaa ggtcagaaag
     6541 gttcgtttga agcaacgatc gttttaaaag aagactttac aacaggcttt gaattttctg
     6601 aaaagaaaaa aacgaactat aagaaaagaa cacgccgcac tacaaaataa tgaaggggtt
     6661 atcctatgcg aaatacaaca cacaaacaac aagaaatcga aaatcaaaaa gcaaaaattc
     6721 gtttagaaca agagaaatta cgtgcaaata aaagaagact gcaacaatta aagaaccagc
     6781 agcgcattca aaaagaaatc gaattagatc gatagacgtt aatcttgtta caaataaact
     6841 actatgatat atttgatgta gtgtatttgt aacgaaggga ggggaagtaa atgcaagata
     6901 aactgaaaga attgttggct attcgcgatg gaaatttatc tatgaaggaa gccaggaaat
     6961 ttggaatcgc agcaaccact gtacaaagat tggtgaatca aggaaaattg atcaaagttg
     7021 atcgtggtta ttatgtagag gatggtcatg gaatagatga tttattttgg cttcagcaac
     7081 gattttccag gggaatcttc tctcatgaaa cgactttaga tatttaccag ctttctacga
     7141 atatgccgaa attcattcac ttaacttttc cccatggcta taacgttgat cgttcaatta
     7201 caaaagagca gcagttacaa tttcatttcg tgaagaaaga agtttatgaa ctaggaaaag
     7261 tagagggaac ttcctttcaa ggaaatccta ttactatgta tgataaagag cgtacacttt
     7321 gtgatatttg gaatccgaga tatgaatttg aatatgaaat gaaattagaa gcattaaaag
     7381 agtacatgga agacccattg agagatccaa gtaaattaag agattacatg acaaaactac
     7441 atgtagaaaa agaaatgaaa taccacatgc aaagcttata ttgatggagg aaaaagatga
     7501 caccgacaca atttgcagca aaaacaagaa atttagcaaa gagtacagga ttaaatgcac
     7561 agttagtgca acggcatttt atgatggatc gtctaattga acaaatttct gaatcaaagt
     7621 acaaaaatga cttcattttg aaaggcggct ttttattagg atcaaaatat ggaatcgata
     7681 gaaggacaac aattgatatc gatacgacat ttagaaatag caaattaact gaagaaaaat
     7741 tgaagagcat ttttaatgat ttaactagaa aacccacaaa agaaggcatt gttttctcta
     7801 ttcaaggaat gaaggaaaca agagaagccg atttttatcc tggatttcaa attaaattgg
     7861 tggctaattt agagaaagta cgtgtaccgt ttaaaatgga cgtgacaaca ggagattcta
     7921 tttatcctga agtagataca cactatcacc aattaatgtt tgaagataaa aaagtggaga
     7981 ttccagctta tccaacag
//
LOCUS       DACCVU010000129         7934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCVU010000129 DACCVU010000000
VERSION     DACCVU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.130"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            354..1415
                     /locus_tag="IVW29_002521"
     CDS             354..1415
                     /locus_tag="IVW29_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7839977.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            1733..2323
                     /locus_tag="IVW29_002522"
     CDS             1733..2323
                     /locus_tag="IVW29_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP7839978.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            2350..3660
                     /locus_tag="IVW29_002523"
     CDS             2350..3660
                     /locus_tag="IVW29_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7839979.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            3657..4376
                     /locus_tag="IVW29_002524"
     CDS             3657..4376
                     /locus_tag="IVW29_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7839980.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            4391..5476
                     /locus_tag="IVW29_002525"
     CDS             4391..5476
                     /locus_tag="IVW29_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7839981.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            5612..7144
                     /locus_tag="IVW29_002526"
     CDS             5612..7144
                     /locus_tag="IVW29_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7839982.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            7322..7543
                     /locus_tag="IVW29_002527"
     CDS             7322..7543
                     /locus_tag="IVW29_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP7839983.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2817 a          845 c         1524 g         2748 t
ORIGIN      
        1 attctggatg atgaagagga agatattatc tgataactta ccctaaactt tgcagggggt
       61 ggttataagt tgtgtatatg tggcagtaat gaatgagtga aataatcata ttaagaaata
      121 aaattgcaat attgtgtaat acaaaagtat attttcatta ttccaaggga aagcatactt
      181 acgaggatca tattattgat ttaataaata atttaaagga aataattttt ctagaagaag
      241 atagtatgtc attcacatca tcaagatcag agtctatttt tttcagagaa tttaagaaaa
      301 tcgttgatgc cgtcattgac taattgagaa aacgtgagtg aaaggataga aggatgaatt
      361 cgagtgatct aattagtgta atcatacctg tatataatgt tgaaaagtat gtaggaagtt
      421 gtttggaatc agtgtgtaat caaacatata agaacctaga tattataatt gtggatgatg
      481 gatctaagga ttcatctaat aaaatatgtg aagactatgc attaaaggat tcaaggattc
      541 gtattattca taaaaataat gctggcttgg gattggcaag aaatacagga cttgaacatg
      601 taaaaggaaa atatgttgct tttattgatt ctgatgattt tgttgcagct aattatattg
      661 aagcaatgtt aaatggtata aaaaggagta atgcagacac atgttatgct ggatattatg
      721 aatattatga tgataataat attatttcaa agccagcaag atatgatagt acgatattta
      781 caaataacgc tataattgaa aaagtgttgc ttggaatgat tggcactgag ccgagttata
      841 aagtcgattt tatattgcca atgtcagttt ggcatgcgct gtattcatct gagataattc
      901 aacagcacaa tatcaagttt ccgtcagaaa gggattatat atctgaagat atcatattcc
      961 atatttcata tttgcaacat tcaaaaataa caagttttat taaagattgt atctattatt
     1021 atagatgtaa taataataat tcattgacac ataattttag agataatgag ttcgattgta
     1081 atattgaatt agttaaaaaa attgaaggcg atttaagcta tatattagaa aatgagatat
     1141 ttgtaaacaa aacaaggaga cttttgttgg gacgctgtcg tacttgtatt caaaaggcag
     1201 ttagatatgc tagttttaat aaagaatttg atttaaactc ctatttgtct aagatactta
     1261 tgaacgatga gttgattgcc tgtattcact catacccata taaacaaaat ccaattaaac
     1321 aacgaatatt taactatttt ttactaaacc aatccattat tggggttaaa acattagcat
     1381 atttaagcgg aatgggtaga aataggagat tttaatcata aaaaactgta ctatagtgaa
     1441 tttttggaga tttatattcc agttatatat taatgattgt gatacttgta ctattgtaaa
     1501 aattcgttag gtttaaataa ctggaaatat cggaaaatgg gcattataca tcacggacat
     1561 cccttctttt ggaaaaaatg ttacgatttt tataagtaat attgtatccg gtggaatcaa
     1621 cgatgaagat aacatatcca tcgttgtaga ttcagtagca ttgaatgatg ctcttgttgc
     1681 aagaaatccg aataaaattg ttaagtataa attgaattaa taggagatat gaatggaaat
     1741 tagcagtaaa gaatatttaa aagcggttct tcgtatggaa aagaatctat atatagaatc
     1801 taaaggacaa cacacaaaat tactaaaaat aatgaaatca catccggact acaacgcttg
     1861 gcggtatact cgtcgaatga gaattacatc ttattattat tcaaagagaa agaaaaatat
     1921 catttatgcg attatgtata ttgtgtcttg cagaagaatg aatagtttgg gaagaaaatg
     1981 tggtatagag tgtggtgaaa atgtatttga tgaaggtgta agattttacc atacacaagg
     2041 aattgttatt aatggtaacg ccataatagg aaaaaattgt tacttatatg ggaataactg
     2101 tattggaaat gatggaatcg atcctaagtg tcctgtaatt ggaaataatg tacgtgtatg
     2161 cgtgggtgca aagattattg gtaatgtaaa aatagctaac aatgtagttg ttgcggcagg
     2221 agctattgtt atcaaagatt gtctagagaa tggtgcaatt cttgcaggtg taccggcaaa
     2281 aataataggg tatgttaatt taacaaaagt atttttagaa taatttttag cgaaagaagg
     2341 tgaataacga tgcgtatttc aataaaaaga tggtcaggag cagattggac attgttcacg
     2401 ttggcatttt ttgcaatctc catagttctt gtatatactt ttaacgcccc aagagcaatt
     2461 ttatatttgg gagatataat taatatcttt gtgttattaa atgcacttag ctttagacgc
     2521 agaaaaggaa tatatgctag caaaaatgag atgctgatag ttttgttttt actagtttct
     2581 atttgtagct cagttattaa tttagaaaat ataggattga ttatctgggg gatacgaaat
     2641 aacactcgta tgttcatagt atatataaat tgtgcaacat atttaacaat taaagattac
     2701 tataaagtat tcgctataat tgaaaaatta ttctggattt cactgccact ttgtttaatt
     2761 gaaagattca tggtcaccta tccaactgga acaattgtag gagatatgat aggtggagtt
     2821 ttttggaatt attcaggttg taatatgcca ttgaatttaa ttttgtgtgt gacagttact
     2881 gttgttacaa tcggatattt caagggagaa aaaaatttca tccattttat tttatgtgtt
     2941 tgtgcagcac tttttatgtc tgcattagct gaattgaaag tttttattcc tgaattattt
     3001 ataatatttg ttgcaatagt attcacttta aaagttgatt ttaaagtagt gatcaagata
     3061 ttatcatgcg taatacttgt gtcgatagta ttatcctttt ttataacaat atttattaca
     3121 ttgaacgata atggacaaag ctatgctgat aatttcacaa ttcaaggtct tttagaatat
     3181 gcgtcaagag attctggata tgatggtgtt ggtgatttaa atcgaataac tggtatctct
     3241 agagtagtag atgaaatatt taataataat tttagattag tcctaatggg agtaggcatt
     3301 ggcaatgcag agtttaccaa tttttttgtc agtgagtttt ataaatcgtt tggtaatctt
     3361 cattatcagt attttcatca tatttggtta tttatagaag ttggaatatt aggagtttcg
     3421 atattcctat tttttataat aaagacattt ttcaatgcaa aaagatcttt tatgaaggat
     3481 aaagatgcaa gtcttgttaa agttattgca attatgtcgg tattcttttt tgtgtataac
     3541 acaacactta ggggtgaaac aacggggcca ctgatttata tgattttagc catgcctgaa
     3601 gttgtgaaaa ggaaagagca ttattatagt gaaaaaaata caaaaggaga gatattatga
     3661 ttcctaagaa gatacattac tgttggttcg ggggaaatcc acttccgaaa aaagatagaa
     3721 agtgcataga aagctggaaa aagtattgcc ctgattatga gattattgaa tggaatgaat
     3781 caaattatga tttaaataag aaccgttata tggccgatgc atataaagaa cgtaaatggg
     3841 gttttgttcc agattatgca agatttgata ttatttataa tcatggtgga ttttatttag
     3901 atacagatgt tgagctaatt aagtcgttag atatacttcg tgaaaatcat gcctatatgg
     3961 gatttgaagg ggaaatctgg gttaatggcg gtattggttt tggtgcagaa gaaggacatt
     4021 ctgttattaa aggtttaagg gatatttacg attcattaag tttttatcaa aatgatggta
     4081 cattgaactt gaaaccgagt ccgtattata ttacagatta tcttgaatct attggattgg
     4141 ttcgcgataa cagcgaacaa ataataaatg gcctgcatat ctatcctacg gattattttg
     4201 ctgccaagga ttatgatact ggaaaaattt ataaaactag taatacaata agcattcatc
     4261 aatataatgc atcttggaaa acacctaaac aaaaatttgt ttatagatta aaatcaacaa
     4321 ttggacaaaa aaaatttaac aggttacttg cactgaaaaa atttttacac ttataaagga
     4381 gaaaagtact ttgaagaaaa tttgtttcat tagtggtgta atttctcgtt ctgggggaac
     4441 tgaaagagta gggagtatta ttgccaatag cttgtctgat ttaggttatg atgtcactat
     4501 tttaagcttt tggaatagtg gagagcctta ttttgggtta gataagacga ttaaggttga
     4561 ttatatgtta aatcctaaaa cggagggaaa attatataga acttatttat atccggtttg
     4621 gaaattgcac aagtatatat gcaaaaatga atttgatata gtaattgata ttgatacttt
     4681 gttgtctttg tatactacaa gggcaattaa aggaacaaag tgcaaattga tttcttggga
     4741 acattttaat tactggacaa tggaaatgct gaaagaaaag aagagattca aggctaaaaa
     4801 attgataaaa aaatatgcaa agaggcttgt ggttctcaca gaagaagatc gtcaaaaaca
     4861 tattgatgaa tatagtttat cagaagattt tgttataact atgccaaatc cgtgtatatc
     4921 taatatcgag tacgaatatc attatgataa aaagagattt cttgcagtag ggcgattagt
     4981 ggaagagaaa aattttgctg acatgataag tgcttggaaa attgtagaaa aaaaagtaga
     5041 tgattgggat cttatcattg taggaaaagg tgaattagaa aaagatcttc gccagcagat
     5101 aaaagattta aatctgcaaa gagtaactat agcagggcat tcagaccatg tggaggaata
     5161 ttataaatct gcatcttgtt atgtgcttag ttctgtttat gaaggatttc ctatggttat
     5221 attagaagca cagtcttatg gattaccagt tatttcttat gattgtaaaa ctggtccgag
     5281 agatttggta catcataatt ttaatgggat gttggttgaa gataagaata tagaccaatt
     5341 agctaagagc atgataatgt ttactaagaa tactgattta gcaatgagaa tgtcactgaa
     5401 cgcatataat aatgtacaaa aatttaattt gaaagaaatt acaaaacaat gggttgcttt
     5461 gattgaaaat gtatagtttt ttgtggttgt ccaaacatga aaattagcgt aattaatcaa
     5521 agagctagaa gaaacctatt acatttcaag attttagaaa ttatatagta ttaggtaaca
     5581 ataaaaaata gaaagtttgg agccatatga tatgataatt aatcgtaaac aaaatgctaa
     5641 aaggaatata attactggaa caatttttaa gttatatcag attatcattc ccttttttat
     5701 gcgtacactt atgatttatc agatgggaat ggagtatgct ggattgaaca acctgttcac
     5761 atctatattt caaatgttaa atttagcgga attgggaata ggtgctgcac taactgtaag
     5821 tatgtataaa cctatagcag aagacaatag aaaagagatt tgtgctttac tgaacgcata
     5881 taaaaagtac ttttcaatta ttggatggat agtgtttaca ttaggattgt tatgcattcc
     5941 ttttttgaga tttttagtta aaggttcgat tcctggaaat ttaaatatga cagttttgta
     6001 tttaatgtat ttgataaata ctgttttgtc gtattggtta ttttcatata gaaagagtct
     6061 aatatatgca catcaaagaa atgatgtaat taacgtgtct atgctaatta cttttacatt
     6121 ccaatttgta atgcaggcat atgttctgat tttcttaaaa aattactatt tgtatttatt
     6181 ggcttctatt ttgggacaaa tagttcttaa cttaatgtgt gctgttgttt ctcaaaagat
     6241 gtatcctgat tatactcctg ttggtgagtt agagaaagaa actattgacg atattataaa
     6301 aaaagtaaaa ggattagtta caaacaaggt aggcggtaca atattacgat cttcagattc
     6361 aattattatt tcatctttct taggtctttc agtacttgcg atttatcaga attattactt
     6421 tattatttct gccataatga gtatatttgc aataatttat gaatcatgta tagcaggtat
     6481 aggtaacagt cttattgtag aaagtcaaga aaaaaattat atagattttt gtgatattac
     6541 aacaattata ggttggtttc tggcaattag ttgtgcatgc tttattacat tatttcagcc
     6601 gttcatgatt atctgggtgg gaagtgataa tcttatgagt atttcatttg ttatatttat
     6661 gatagttcat tttttcttat atgaaattga tcagttaatt gggttataca aagatgctgc
     6721 tggaatatgg tacgcagaca ggtttagacc attaattaca gcaatgataa acattgtatt
     6781 aaatatcatc ctcgttaatt ggttcggatt atatggagtt ttggcggcaa ccattgtttc
     6841 agtattgttg gttgatttac cctggttatt atataatgtt ttccaaaata tatttaaatt
     6901 tgaatcggca ggtaaatatg tttggaagct cataagtttg tgtgtaatag cttttgttgc
     6961 ttcttattta agttattttg cttgttcgta tgttaatatc ggaaatttca aagtgataag
     7021 tttagtgata aatttagtta tatcggttgt catatcgatt ttagttattc ttctgtttaa
     7081 tatccgtaat aagaagttta agtatatttt gaaagttctt acgtcggtat tggtaaaaag
     7141 atgagaagag acatcagaat gatatataaa gtaactacaa tttttaggct gccgaatagg
     7201 cgaacgatgt atagtttttg ttccaacgaa gactcttgtt gaaattggag atgatatgaa
     7261 ggagttacaa ttttgactca tgtttatgat tgatcggttt taaaagtagt atggagaagc
     7321 tttgggatca tcggagagga tcaaaatagg taataatgtt tttataggaa tgaagtctag
     7381 aatactaaag ggtgtacata taggaaataa tgtagttata ggtgcaaata gtttagtaaa
     7441 caaggacgta tcagacgatt gtgttgttgc aggaaatcta gcaaaagtaa ttatgtccct
     7501 agatgaatat tataaaaaga taaacaatat gaagaatccg taaaacttgt gaaactatac
     7561 cgagaataat atgggaaaaa tattgatgaa aaagcattat atgagttttt agggctgttt
     7621 acagatggag atgacaattt gtcgaagagt tgggagcaat gcaaaattta gtaggggatt
     7681 ataaacagac tgacaaggta tttaaagaac atgcacaagt atttcataat attgaagaat
     7741 ttttgaatac tgtgagatag gagttgctac attgagaaaa gtatgtattt ttccaatgta
     7801 aaagattacg agtttgaaaa cgacaccata atattatttt ttaagaagat attatagagc
     7861 ttaggaacgg aagtttattt aaggatgttg aataaaatga aaaataagat taggtggatg
     7921 aaaaaggcag atga
//
LOCUS       DACCVU010000130         7873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCVU010000130 DACCVU010000000
VERSION     DACCVU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.131"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            58..759
                     /locus_tag="IVW29_002528"
     CDS             58..759
                     /locus_tag="IVW29_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7839984.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1368..2384)
                     /gene="tsaD"
                     /locus_tag="IVW29_002529"
     CDS             complement(1368..2384)
                     /gene="tsaD"
                     /locus_tag="IVW29_002529"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7839985.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2381..2845)
                     /gene="rimI"
                     /locus_tag="IVW29_002530"
     CDS             complement(2381..2845)
                     /gene="rimI"
                     /locus_tag="IVW29_002530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7839986.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2852..3394)
                     /gene="rimI"
                     /locus_tag="IVW29_002531"
     CDS             complement(2852..3394)
                     /gene="rimI"
                     /locus_tag="IVW29_002531"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7839987.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3378..4202)
                     /gene="tsaB"
                     /locus_tag="IVW29_002532"
     CDS             complement(3378..4202)
                     /gene="tsaB"
                     /locus_tag="IVW29_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7839988.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4291..5271)
                     /locus_tag="IVW29_002533"
     CDS             complement(4291..5271)
                     /locus_tag="IVW29_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7839989.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5295..6779)
                     /gene="galT"
                     /locus_tag="IVW29_002534"
     CDS             complement(5295..6779)
                     /gene="galT"
                     /locus_tag="IVW29_002534"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7839990.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6791..7780)
                     /gene="galE"
                     /locus_tag="IVW29_002535"
     CDS             complement(6791..7780)
                     /gene="galE"
                     /locus_tag="IVW29_002535"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7839991.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2210 a         1696 c         1396 g         2571 t
ORIGIN      
        1 aaattcttta aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg
       61 cgtgttaata aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat
      121 ttctcggatg aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa
      181 ggacagatcc ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga
      241 ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat
      301 acaaataacc tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct
      361 caagcgatga ctgatattga aacattctac cttcctgttt cctactatga aaaattagca
      421 cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata
      481 catgaattgc gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat
      541 ttattcattt tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct
      601 tatccgatca ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga
      661 caagtaatca aacaactacg tgattccggg ctgttagatt atgacaaaaa gcgttttact
      721 ttctgtaaga cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg
      781 accctgataa atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg
      841 cagttttaga tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata
      901 ttcactgttt gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga
      961 tctagattat tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa
     1021 cggataatga taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt
     1081 aaaaaatagg gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc
     1141 tgtcaactct tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat
     1201 tgttccttgt gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca
     1261 aaagcttgca tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc
     1321 tgggctgttc ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa
     1381 accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca
     1441 tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact
     1501 agttctacct cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga
     1561 ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga
     1621 agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt
     1681 ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt
     1741 aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa
     1801 ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct
     1861 aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg
     1921 atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga
     1981 agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc
     2041 atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa
     2101 ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa
     2161 tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct
     2221 acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt
     2281 tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt
     2341 tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt
     2401 ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt
     2461 tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac
     2521 gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca
     2581 aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga
     2641 cttcatctat tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa
     2701 gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg
     2761 aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat
     2821 caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat
     2881 ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc
     2941 ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg
     3001 tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc
     3061 gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg
     3121 ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag
     3181 gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat
     3241 agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta
     3301 cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa
     3361 agcttctggc atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc
     3421 tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc
     3481 tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc
     3541 catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat
     3601 ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac
     3661 aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag
     3721 cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta
     3781 tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta
     3841 tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa
     3901 ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca
     3961 aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt
     4021 atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta
     4081 tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt
     4141 gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac
     4201 atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat
     4261 cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa taatcaactc
     4321 tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt
     4381 ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa gcgagggcgt
     4441 tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt ctggtaccac
     4501 taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa
     4561 aagcgcatca ggaacatgcg gattcgtttt taaatactgc ttcactgctt gatatcctga
     4621 ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt ctgccaaaac
     4681 ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct tcgtatattc
     4741 tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt caatgacttt
     4801 ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag tgccgtcaga
     4861 atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag cttcgtcgaa
     4921 ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc acgtttcttt
     4981 caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa tagataaata
     5041 ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt
     5101 agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg cttcaaaaat
     5161 tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa ctcttgaaac
     5221 agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca tttgtctctc
     5281 ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg tcctttttca
     5341 tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc tacagatttt
     5401 tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt taattgttct
     5461 gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa atgctctact
     5521 tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac ttcgatcaac
     5581 ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat cccatcagga
     5641 tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta attgtccctt
     5701 cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata
     5761 gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc tgcttcaata
     5821 agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt cacgactcct
     5881 gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc ttttgccata
     5941 ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc tccaacaatt
     6001 ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt
     6061 ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa aatgatcgaa
     6121 tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc gtctagattc
     6181 attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct atatccttcg
     6241 ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg ttctgctgcg
     6301 atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag ttcaccatac
     6361 tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat gtagtcattt
     6421 ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga atagtgctgt
     6481 gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca
     6541 aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc cttagcgaca
     6601 agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat gctttctaga
     6661 gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc catttccatc
     6721 catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact gattgacata
     6781 tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc catgctgttt
     6841 tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct ctcgcttttt
     6901 cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt tctgctggaa
     6961 tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag tatcctttgc
     7021 tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc aaagctaaaa
     7081 tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt ccatctggtg
     7141 tatcataatc gtctccataa acagccaagg cttttctttg accaagagct acttgtaaaa
     7201 tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca tctgcttttg
     7261 ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct tggtcacacc
     7321 acttcatcat tttttccatc atcaatttac tttcaccata cggattttta ggattagctg
     7381 gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca gtagaagaga
     7441 atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc ataccgtata
     7501 cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa gagcttgcag
     7561 caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga aattctttat
     7621 cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg tgacctgtga
     7681 gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct acagcgtgcg
     7741 aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact cctttgatta
     7801 gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt ttatggtgaa
     7861 aacaaacaaa gta
//
LOCUS       DACCVU010000131         7839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCVU010000131 DACCVU010000000
VERSION     DACCVU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.132"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            98..1864
                     /locus_tag="IVW29_002536"
     CDS             98..1864
                     /locus_tag="IVW29_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7839992.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            1966..3225
                     /locus_tag="IVW29_002537"
     CDS             1966..3225
                     /locus_tag="IVW29_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7839993.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3372..4673
                     /locus_tag="IVW29_002538"
     CDS             3372..4673
                     /locus_tag="IVW29_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7839994.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4676..5527
                     /locus_tag="IVW29_002539"
     CDS             4676..5527
                     /locus_tag="IVW29_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7839995.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5540..6355
                     /locus_tag="IVW29_002540"
     CDS             5540..6355
                     /locus_tag="IVW29_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7839996.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6506..7672
                     /locus_tag="IVW29_002541"
     CDS             6506..7672
                     /locus_tag="IVW29_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7839997.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2537 a         1360 c         1573 g         2369 t
ORIGIN      
        1 caaaaaactg ttaccgataa catttttgaa agcgttgtct tttgtttctc tatcacttat
       61 agtgatgacg tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca
      121 tcgaccggaa agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg
      181 tactaggaaa aatgatatca aaaaagtata tgtgctttgt ggagatccat ataccataac
      241 aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca
      301 tgattattgg gaaattgaag tgacgagcga aacaaggcgc ttacagtatg cgttccatgt
      361 tgttggggaa gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga
      421 aaatgtgatt actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg
      481 attcactatt ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt
      541 tgcaaacggg gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc
      601 agaccgagaa gacttcttcg ggggagattt gcaaggtgtg agggatcatt tggattactt
      661 gacggacctt ggcgtcaatg gcatctattt ttgtcccata tttaaagcca catccaatca
      721 taaatacgat acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt
      781 aaaaaacttg attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt
      841 caatcatatg ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc
      901 gatctataaa gactggttcc acatccgttt tttcccagta gattcttatc agatgactga
      961 cttaccagaa actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc
     1021 gaaactaaat acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg
     1081 gataaaagaa ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca
     1141 tttttggaaa aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg
     1201 agagatttgg cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa
     1261 ttatgcattc acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca
     1321 aatggtcagt ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac
     1381 attcaactta ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa
     1441 agaattgatg aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta
     1501 ttacgggaca gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat
     1561 atgggagaag gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct
     1621 aaggaaacag ttgtctaaga ttatttctga aggttctact cagtggctaa tagtatatga
     1681 tcgtgaagat aagctctatt tcacacggga actagaggga cagatcatct atgtttactt
     1741 taatcagtca aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa
     1801 ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg
     1861 ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta
     1921 taataagggt gtaacagcac gttgaataca caggaggaaa tgaagatgaa aaaaagaagt
     1981 gtgaaacagt tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt
     2041 ggcaatggta actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc
     2101 gttgattctg gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac
     2161 gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta
     2221 gatgggcctg ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg
     2281 ttaggacagc aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa
     2341 acagacaaag cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag
     2401 acccttgttt tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat
     2461 ttggagaatc tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca
     2521 ggatttttag ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt
     2581 ggctatgtat ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt
     2641 gctgtagaag gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg
     2701 caagacataa aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca
     2761 gcaatcattg acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt
     2821 gtagccaaaa ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa
     2881 ggatgggtag tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat
     2941 gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac
     3001 caacaagcta gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa
     3061 caatatgaaa cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt
     3121 gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat
     3181 gaagcagtaa aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg
     3241 tccattttta taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa
     3301 aattatcttg ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag
     3361 ggggcattca gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt
     3421 gttcacttat tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt
     3481 ttttcgccat attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg
     3541 taggattggt tactttggga agtataccaa tggaagatca ttcattattt attttgattg
     3601 aaggcacttt gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt
     3661 tagacgctta tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga
     3721 aggaagtcat acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt
     3781 atcttctcat gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta
     3841 ctaattatga cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa
     3901 acttttttag tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt
     3961 ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag
     4021 cggttgttgc tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat
     4081 taccatgggc ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata
     4141 gcgtaggtgc catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata
     4201 tcagtgcaat ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc
     4261 agggctggtt aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt
     4321 cagaagactt atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa
     4381 acatcacgct tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg
     4441 ggaatttcaa taatttctcc atgatctatc tgtttaacaa tggtggtccc ggaagcgtag
     4501 gcggaggagc tggttctact gatattctta tttcctggat ctataaactt acgactggaa
     4561 cttctccgca gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct
     4621 gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa
     4681 aaaatttcat tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt
     4741 tcttttaata ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt
     4801 caagtcagga aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa
     4861 ctttactcgg ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat
     4921 ttctgtccta acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag
     4981 tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt
     5041 accgactatg gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga
     5101 taagtactgg ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct
     5161 gatgaaagga tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg
     5221 ggcagggcat ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc
     5281 cgttcaagca ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct
     5341 gcttcgaaca cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt
     5401 gacaaaccaa agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg
     5461 tctattattc ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa
     5521 aggataggag cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac
     5581 gccaactcaa gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt
     5641 tgtattttta gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca
     5701 agcgattcca ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca
     5761 aaaattttct gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc
     5821 tgtgacccct gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg
     5881 aacttttatt gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat
     5941 acgccgctat acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga
     6001 agatttccaa cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt
     6061 atcttatagt ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg
     6121 ggcttccttt ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa
     6181 agagagtgca aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt
     6241 agggtttatc cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt
     6301 gctgctttta tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt
     6361 ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa
     6421 atttctgaaa aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag
     6481 ggtagttttt ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc
     6541 acgttctttc tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg
     6601 agatacggac actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga
     6661 gattacaagt aacgcttctt tggcactagg agaagcctat atggatcatc gaatcgagat
     6721 cgagggaagt atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg
     6781 aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa
     6841 agaagatatc catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc
     6901 aactttgact tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca
     6961 aatcaataaa gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct
     7021 tgatatcggt tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa
     7081 ggcaactggg atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga
     7141 agaacattta gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga
     7201 aacgtttgat catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga
     7261 aggttatttc aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg
     7321 aatcagccga caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg
     7381 cggttatgtc cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact
     7441 gattgatttg gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa
     7501 ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg
     7561 ggatctatat ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca
     7621 atatttgctt gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca
     7681 aaagtactgt ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa
     7741 cttttttgtc taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc
     7801 ataataaaag tttggttctt taatttttaa acatgacag
//
LOCUS       DACCVU010000132         7660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCVU010000132 DACCVU010000000
VERSION     DACCVU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.133"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..788
                     /locus_tag="IVW29_002542"
     CDS             51..788
                     /locus_tag="IVW29_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7839998.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            802..1617
                     /locus_tag="IVW29_002543"
     CDS             802..1617
                     /locus_tag="IVW29_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7839999.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1630..2277
                     /locus_tag="IVW29_002544"
     CDS             1630..2277
                     /locus_tag="IVW29_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7840000.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2293..2949
                     /locus_tag="IVW29_002545"
     CDS             2293..2949
                     /locus_tag="IVW29_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7840001.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3111..3932
                     /gene="racE"
                     /locus_tag="IVW29_002546"
     CDS             3111..3932
                     /gene="racE"
                     /locus_tag="IVW29_002546"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7840002.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3935..5290
                     /gene="rph"
                     /locus_tag="IVW29_002547"
     CDS             3935..5290
                     /gene="rph"
                     /locus_tag="IVW29_002547"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7840003.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5291..5809
                     /locus_tag="IVW29_002548"
     CDS             5291..5809
                     /locus_tag="IVW29_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7840004.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5916..6416
                     /locus_tag="IVW29_002549"
     CDS             5916..6416
                     /locus_tag="IVW29_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7840005.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6696..7616)
                     /locus_tag="IVW29_002550"
     CDS             complement(6696..7616)
                     /locus_tag="IVW29_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7840006.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2493 a         1328 c         1653 g         2186 t
ORIGIN      
        1 tatacatctt taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga
       61 ttaaatttga tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc
      121 tcgaattcga gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca
      181 cgatgcttcg ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg
      241 atactgactt gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg
      301 ttttccaaca tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac
      361 caatcaaagt gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga
      421 aaactgtcaa tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac
      481 agcaacgggt ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg
      541 aacctacttc tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat
      601 tggctcatga cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag
      661 tggcggatcg tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga
      721 gagacttttt cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca
      781 atcattgata ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta
      841 ggcattgctt ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt
      901 ttagagcgaa gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta
      961 tttggaatga tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa
     1021 gcaatcacta aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct
     1081 aaaactcgga ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg
     1141 atcacagatg aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa
     1201 gctttgttgg taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca
     1261 acggttctag cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat
     1321 gcaaaaattt tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag
     1381 ggagacgcga tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag
     1441 tatgagttag ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg
     1501 caagaaaact tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca
     1561 tacgataaaa tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg
     1621 ggagaggcaa tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag
     1681 atcacattgt tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg
     1741 gcaatcttcc aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa
     1801 ttttttagaa acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt
     1861 tattggttta atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct
     1921 gcttttattg ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa
     1981 gcaggactat cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa
     2041 gcattcaaaa tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca
     2101 tctattttag caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt
     2161 gaaacattca ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg
     2221 ctaccgttat cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag
     2281 gaggggaaat acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg
     2341 gaaggattaa aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc
     2401 ggtggtctga caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt
     2461 gtagtgattg atattatacg gaatcttcca ttattattga tcattttctt cacttacttt
     2521 gccttacctc aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg
     2581 atttttgagt cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa
     2641 ggacagatgg aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc
     2701 gtgttaccgc aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg
     2761 atcaaagata cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt
     2821 attatttatg gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg
     2881 tacttcgtgg tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac
     2941 agttattaat agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc
     3001 tctttccatt cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat
     3061 tcacgctaaa aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat
     3121 tgacagataa tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa
     3181 aagaagccct gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct
     3241 gcccatatgg ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt
     3301 atctggtgga gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg
     3361 ctttagaaga aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta
     3421 ctagagcggc agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta
     3481 cggtaaaaag tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga
     3541 caagtcttgc ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg
     3601 tggcgaaaaa aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat
     3661 tgattttggg atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag
     3721 aaaatgttca actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag
     3781 attatttcaa tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa
     3841 ctggctctgc caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa
     3901 atgtagaaca tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca
     3961 agagcttcgc aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt
     4021 gatccgtttt ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc
     4081 ttttttgaga gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc
     4141 aacgagtacg agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga
     4201 aatccagcgt cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga
     4261 acgtagtatc atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag
     4321 tatcactggg gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga
     4381 attgtcagaa gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga
     4441 tgggacttgc gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa
     4501 tcttgtaatg acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac
     4561 gtttgatggt gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt
     4621 gatcaaagag caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga
     4681 agaaacaaaa acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa
     4741 tatgttcaaa gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga
     4801 tgtggaagaa actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc
     4861 acaattgctt gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg
     4921 cggtatgcca ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa
     4981 taacgccaaa ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt
     5041 ccattgtacg ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg
     5101 gcccggtcga gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt
     5161 tatcccagaa ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat
     5221 cagtcatcgg gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg
     5281 agagagatag atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt
     5341 tgacttagta gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga
     5401 attagaagct tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta
     5461 tggaccagga ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac
     5521 tcacggtcat ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc
     5581 tgcaactgcc gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca
     5641 gaaaatcctg tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc
     5701 atcatatgct cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc
     5761 ccatcaggca gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact
     5821 ctaattttta ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata
     5881 gtagtgttga atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa
     5941 tgctgctgga aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt
     6001 atcatcatcc attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc
     6061 ctgtattagg aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata
     6121 aaatgattga cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg
     6181 tgacagaaac agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt
     6241 tgttagtcga ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca
     6301 ttacaagaaa agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta
     6361 atatattgtc tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa
     6421 cgagaaacta aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt
     6481 atcttgaaaa agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaattcacg
     6541 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
     6601 ccgccgttta tacgtgttta gagcgtggga caaaagtgtt ctttactttt gtcccacgct
     6661 ctttttagtg agattcgttg catcgtttgt ccaaattatt ttccgacagc aattggactg
     6721 ttcggaatcg tgaaaccaga agctgtcaat tctttgacat aggagctcaa gaactcttct
     6781 tttaaggcat attgttttcc gttcaatgcg tagcagaccg tacgaatcgc aaaattgctg
     6841 tttccaagat ccactaatcc aaagatagaa ggttcagttt ggagctcttc ttggtatttt
     6901 tcagctaatg tacgatttac tcgatcaatg atctcataga ttttgtcata tccttcttca
     6961 ggaacaatcc gaatatctaa aagtacttgc atatttgcac gagagagatt gctgatcgtg
     7021 gtgatgtttc gattcgggat gaaatggaca gtaccatccg ttgctttcag ctgaagggta
     7081 cggataccaa cagatacgac ggttccttca atcgttaagt ttgctagctt gatgtagtcc
     7141 ccaacatcca tctgttgttc catgataata aaaaatccgg taataacatc actcatgaac
     7201 ccttgggcac caagaccgat tgccacacct gcaattccgg caccagccaa aagtgatccg
     7261 atcggcacac cgatagcaga taaaattgca tagataaaaa agaaaccaag ggtataatga
     7321 aaaatagtat ttaaaagact atgtaaagtc tttaagcggc tctcgtttag aatggctttt
     7381 ttagaatact gattaaaaga cttatcaatc agatattttc ccacacggtg aagaatccaa
     7441 aaaaggacga tcaaaaaaat cagatataaa cttttttgga taaaggcagc caaaatggcg
     7501 tcccaatcaa tcccgtccca aaaccgctga aacgcattta ctttgctgac agcttgatct
     7561 gtcaatgtct cgccagaaga gtctgtcgta ccggcagttg ctaataatga aaacataaaa
     7621 tcctcttttc taaaatttga ttctttctgt attgtaccaa
//
LOCUS       DACCVU010000133         7537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCVU010000133 DACCVU010000000
VERSION     DACCVU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.134"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            107..1248
                     /locus_tag="IVW29_002551"
     CDS             join(107..335,335..1248)
                     /locus_tag="IVW29_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7840007.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="IVW29_002552"
     CDS             1483..1935
                     /locus_tag="IVW29_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840008.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="IVW29_002553"
     CDS             1949..2638
                     /locus_tag="IVW29_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840009.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="IVW29_002554"
     CDS             2763..5114
                     /locus_tag="IVW29_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7840010.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="IVW29_002555"
     CDS             5194..5589
                     /locus_tag="IVW29_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840011.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="IVW29_002556"
     CDS             5914..6531
                     /locus_tag="IVW29_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840012.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="IVW29_002557"
     CDS             6572..6871
                     /locus_tag="IVW29_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840013.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="IVW29_002558"
     CDS             6905..7072
                     /locus_tag="IVW29_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7840014.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="IVW29_002559"
     CDS             complement(7117..>7537)
                     /locus_tag="IVW29_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840015.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DACCVU010000134         7373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCVU010000134 DACCVU010000000
VERSION     DACCVU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.135"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..318
                     /gene="ssb"
                     /locus_tag="IVW29_002560"
     CDS             <1..318
                     /gene="ssb"
                     /locus_tag="IVW29_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7840016.1"
                     /translation="MATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQLLE
                     SKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISDDD
                     LPF"
     gene            684..944
                     /locus_tag="IVW29_002561"
     CDS             684..944
                     /locus_tag="IVW29_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840017.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            1385..1591
                     /locus_tag="IVW29_002562"
     CDS             1385..1591
                     /locus_tag="IVW29_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7840018.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1591..1842
                     /locus_tag="IVW29_002563"
     CDS             1591..1842
                     /locus_tag="IVW29_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840019.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLSPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            1858..2295
                     /locus_tag="IVW29_002564"
     CDS             1858..2295
                     /locus_tag="IVW29_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840020.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNVKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2740..2919
                     /locus_tag="IVW29_002565"
     CDS             2740..2919
                     /locus_tag="IVW29_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840021.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3232..3495
                     /locus_tag="IVW29_002566"
     CDS             3232..3495
                     /locus_tag="IVW29_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7840022.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            3489..3839
                     /locus_tag="IVW29_002567"
     CDS             3489..3839
                     /locus_tag="IVW29_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7840023.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3863..4477)
                     /locus_tag="IVW29_002568"
     CDS             complement(3863..4477)
                     /locus_tag="IVW29_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840024.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            4927..6252
                     /locus_tag="IVW29_002569"
     CDS             4927..6252
                     /locus_tag="IVW29_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7840025.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PVTDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTAIVHASSMLEGARSITRSTLVGGHAGGNGGLKND"
     gene            6245..6595
                     /locus_tag="IVW29_002570"
     CDS             6245..6595
                     /locus_tag="IVW29_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840026.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANHNLAKEQMTTE
                     EIAEVLNAAVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKYKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            6592..6771
                     /locus_tag="IVW29_002571"
     CDS             6592..6771
                     /locus_tag="IVW29_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840027.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            6907..7200
                     /locus_tag="IVW29_002572"
     CDS             6907..7200
                     /locus_tag="IVW29_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7840028.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFIYDLSLASSCNMSDVYEKLLILENEYDQFNSNQKKKKKKHID"
BASE COUNT         2567 a         1189 c         1432 g         2185 t
ORIGIN      
        1 atggcgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
       61 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
      121 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
      181 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
      241 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
      301 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
      361 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
      421 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
      481 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
      541 aacaaaatca aaaagaattt tcaaatctat aacctcctcc ccttgccaga ttaggccggg
      601 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
      661 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
      721 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
      781 attgtgtagt tgcagatatt gaaattatcg ataaactgct aattatggat gatggaaaat
      841 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
      901 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
      961 agtcctaatg taggactgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     1021 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     1081 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     1141 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     1201 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     1261 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca
     1321 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     1381 aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     1441 tcatgcacca catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     1501 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattacat
     1561 tttgattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta
     1621 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcggaagct
     1681 ttacttagtc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
     1741 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
     1801 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
     1861 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
     1921 gaggatatga cgttcctata caacgaaaca cgtgttccaa agaaacatta taaagaaaaa
     1981 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
     2041 tactatcgaa cactggaaga actgaaaaag caaaatgtaa aatggttttt ccaagcctta
     2101 ctatgcttgg aagtaggcgt caaaccaagc acaatcaaac cttctgaata ccaagcttta
     2161 gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca agactgtttc aagtgaatgg
     2221 ttagattatt ttgaaaatat caataagtat ggcgcatatt acaccatgaa aaaggaagac
     2281 aacgaaaatg aataaggtga tttatcaact aacaacggat tccatatcaa cgatttgaaa
     2341 gaaaaaaagg tcaataatca tttataccga aaagtgagaa tgtgtgaaat aaggacaaaa
     2401 aaagcctagt aactaaggga atggttctta tttcgcacca atcgctccaa ccgcccccgc
     2461 cgcttaggtg atgcgaaata aacgggcaaa gcccgataac ttaagagaaa gaaggtgatc
     2521 catttgaaca agattattcc cttattgtta gtactgtttg ttggcgaaat aattatgcga
     2581 catagtgcag ctgtgcattt aaacttcgta gtatttttgg taatactggt gttttgtttg
     2641 atcttttggc gctttgtgtt agcccttgtg agtctaatat tcaagttgat tgcagccata
     2701 tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc aataggaaat
     2761 aaacggttac ccgttacgtt ggatgaaaag agacaaaaag aattgcagaa actaaagcag
     2821 aagtacggaa aaagtgaatc taagattatg tgtattgcgt tagatctatt gatcgcgcaa
     2881 gaaaaagcag gatttgatgt accagcactc aaaaagtgac gtcacttttc atttgaagaa
     2941 acaaaagtga tagcacttaa ttgttcctac aatttagaat attttctatg tcctttttat
     3001 tccattttgt atcaataact atgaaatctt taaagtatcc agaagagatt atttttagat
     3061 caattttttt cttacctgat tctaaagatt ttgttttctt aaacatatta tgatttcctt
     3121 tcgactattt tcatataaat gttaacatag tttattagaa aaggataatt aagtatatac
     3181 gttttttctt atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg
     3241 ttacctataa aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa
     3301 tatcttggaa ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag
     3361 aagcggctaa ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt
     3421 gaaaattaca ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt
     3481 aatgaaaaat ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc
     3541 gatctggaca cgaacagaca ggtaaaaggc cttatattgt tttaagtcat gatattattg
     3601 cggattatag taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc
     3661 tatatgtaaa tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc
     3721 aactaacgac aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata
     3781 gtttagttga ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa
     3841 atagaaacag cagcatgatc aactattcgt gattgaccgt gctatctttt tttattctat
     3901 atacggtttg tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt
     3961 ttagatcctc aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac
     4021 tgtatagctt aggacgtcct ttataaacac cgttcgcttt cgcaatctta atgccttgtg
     4081 cctgacgtcg tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga
     4141 tcaaatcttt aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta
     4201 gacttgtaat gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct
     4261 cgtcgtaatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
     4321 cgtaatccaa tacttcttgg aaaacaggtc gatgagtaat tgtagcgcca gattgtttct
     4381 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aacttggcgt tgttcatttt
     4441 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
     4501 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
     4561 tctatttagg gtatacctta tatagacaaa aactgatgac ggaggaaata gtatgagaat
     4621 attttgtaag tgtctactga taagttattc tcaaatgttg taaggatttt attcatttgt
     4681 taatattaaa gaacggaact ccaaaaaagt ccgtactcta atttgcagtc tctcactctt
     4741 ttagagtgag agtttttatt agattttcaa attaattagt aaagtgatag aataaattga
     4801 tcatacaaat caaaagaaga agcagggtga tagcatacct atgcttcttt ttttatgtat
     4861 ttagctattt cttctttaca atatcgaaca tatgtttgta taatgtgaaa gaatggagtg
     4921 ttatttatga tggattattc aaaagagcca gtaaatgatt actttttaat cgatatgaaa
     4981 agcttttacg caagtgtcga atgtatagaa agaaatttag atccattaac aacagaactt
     5041 gttgttatga gtcgagctga taatactggt tcaggattga tattagcttc ttctcctgaa
     5101 gcaaaaaagc ggtatggtat tacaaatgtg agtagaccac gtgacttacc acaaccattt
     5161 cctaaaacac tacacgttgt tccaccacgt atgaacctat atatcaagcg aaatatgcag
     5221 gtaaataata ttttcagaag atatgtggct gatgaagatc tactgattta ctcgatcgat
     5281 gaatcaatct taaaagtgac ccgatcactg aatcttttta cgactgaaga aacacgaagt
     5341 cagcgaagga agaagctcgc tcaaatgatc caagaacgta ttaaagaaga gttaggtttg
     5401 attgctacgg taggcgtcgg cgataatccc cttttggcta aattagcctt agacaatgaa
     5461 gcaaagcata atgaaggatt tattgcggaa tggacgtacg aaaacgttcc tgagaaagta
     5521 tggaatattc ccgaaatgac ggacttttgg ggaattggtt cgcgaatgaa aaaaaggctc
     5581 aaccaaatgg gaattttgag cattagagac cttgctaact ggaatcctta tacgattaag
     5641 aatagattag gtgtgattgg attacagctg tatttccatg ctaacggaat cgatcgaaca
     5701 gatatagcga tcccaccaga accgacaaag gaaaaatctt atggtaattc acaagtgtta
     5761 ccacgagatt atactagaag aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa
     5821 gtagccattc gtatcagaca gcataattgt aaaacaggat gtgtccattt acacattggc
     5881 acgtcgatct tagaaacgag accaggattc tcgcaccaaa tgaaaatacc tgtcacagac
     5941 aatacaaagg aattgcagaa ttattgtctt ttcttatttg ataagtatta tgaaggacaa
     6001 gaagtaaggc acgtcggaat aacctatagc aagcttgtgt acaccgattc gcttcagctg
     6061 gatttattta gtgatccgca aaaacaaatc gatgaagaaa acctggataa gattattgat
     6121 aagatcagac aaaaatatgg ttttacagcg attgtgcatg cttcttctat gctagaaggc
     6181 gctcgtagta ttactagaag tacattggtt ggtggccatg caggaggcaa tggggggctt
     6241 aaaaatgatt gattataacg atcgtggacg gttgaaatgg gcaggattct atttgtccga
     6301 tcacacggaa gagatcgatg cggacaatga gcaacgtgca aatcataatt tggcaaaaga
     6361 acaaatgacg actgaagaga ttgcggaagt actgaacgct gcagtcataa aaaatagatc
     6421 agtatgtatt caaaaagaag aacgaaacgc tgaaggatat tatccagacg atattgttgg
     6481 aaagatctta ggtagtgatg agctggggat ctacgttggc aaatacaaaa ttcactacga
     6541 cgaaatacgt catatcgaat tttaccagga taataaatgg agtcgattag aatgaacatt
     6601 atccagcaat atgagctaaa gtatataacc tttgatcagc tatcagaaga gatttggggt
     6661 tatggccaac gtttgataaa tgaggttggt gttgagcgct tctctttcta tgtagaagct
     6721 gcagcaggtt atcataattt taggttttat atcttcccgt tatatatata aggtatttta
     6781 cggattctat agagttatct ctatgaatcc ttttttgttt ttacaaatag gagtagatac
     6841 tagaataatg cgtgtgttaa actattattg caaacgcaat aataaaagaa agtaggctta
     6901 cgcaccatgt ctatcaatac atttgtttac tcgcacccga tcaatgttta cattatcaag
     6961 aatttaggaa tcactgtaga acaattttgt gagttatatg cctacccaca aggaacggtt
     7021 gcaagctgga taacccgaca acgtagaata aaatcactac ctgcttcatt tatatatgat
     7081 ctgagtcttg cttctagttg caacatgtct gatgtctatg aaaaattatt gatactagag
     7141 aatgagtacg atcaatttaa ttccaatcaa aaaaagaaaa agaaaaaaca catagattaa
     7201 tattgaaacg aaaaatagta tttattttaa aaaaagagaa aggagaaaaa gatatttttc
     7261 tattatttga ttaaaattac ttttaatcaa aactattgta aaatagtact tgttttatgt
     7321 atcttaaaat caaaaaaact ttgattaaag attcttcaat caaaaaaact taa
//
LOCUS       DACCVU010000135         7159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCVU010000135 DACCVU010000000
VERSION     DACCVU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.136"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(46..3195)
                     /locus_tag="IVW29_002573"
     CDS             complement(46..3195)
                     /locus_tag="IVW29_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7840029.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3209..4426)
                     /locus_tag="IVW29_002574"
     CDS             complement(3209..4426)
                     /locus_tag="IVW29_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7840030.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4416..6011)
                     /locus_tag="IVW29_002575"
     CDS             complement(4416..6011)
                     /locus_tag="IVW29_002575"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7840031.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6136..6921
                     /locus_tag="IVW29_002576"
     CDS             6136..6921
                     /locus_tag="IVW29_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP7840032.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2082 a         1454 c         1183 g         2440 t
ORIGIN      
        1 tttactacta tctttgaatc gtacaatttt cttaactctt ctcgtttacc tcaattcatt
       61 ttcaagtgga atcaactcat catctaacat tttacgccat gctcgcttca tttcttgtgg
      121 gtatttaaat ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt
      181 gataattgct ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc
      241 atgtaattct ttctcagaaa caaaccactc attggaaaag gcatgaatca aattatcact
      301 tgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat ctaaagtccc
      361 cacatcaaat gcatttaaaa tttgtcggta aacttctttg actccttctg ttttattcaa
      421 tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgcag cataagttcc
      481 tagtaattga tcaatataag tagaatcaat atcatacaac ttagtggtat tttcactata
      541 aaattcgatc gttgaaaaat cttctccctc tttttctggt tcttcctcca tcaatgaacc
      601 cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat tgttcaaagt
      661 tgaagattgc accatgtcat cattgtaatc atcataagtc actaacgatt catacgcacg
      721 attcaattct tgaaatgctt tgacgtactc gatacggatt tcaagtggcg tatgttcatc
      781 aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga atcttttttt
      841 ggattcttgg taacttggat aaatcaaacc cgattcttct ttagcttctg aatacagttt
      901 tgtcgcatcc tcgacattct tctccattgt cgctggcttt cggaaagtaa caattagtcc
      961 ttttgttttt tcaggatacg tacggttcgt acgtgaaaag gcttggatca atcctgcata
     1021 ctctagatta cgatccacaa atagggtttg aatcgtaggg gcgtcaaaac ctgtcaatag
     1081 acgatccact acgattacta aatcgatttg tttcccaaat tgtttgaatt ccgctctttt
     1141 tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta gtgaccaggc
     1201 agtatcataa tactgattat attcctcaat aattttttgc atttcttctt gttgggcact
     1261 tgaatctcgg ctattttctt ctaatgaata ggtaatagcg attcgtggga aatcaggatc
     1321 ctccatcgtt cgtccttcac ggataggctg attgggaaac tcttttatta accaatcaga
     1381 ttctttcgtc atctctttga tggcccgata atactttttg gccatatcaa tggaactcgt
     1441 ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg tataggcatt
     1501 gtctggacgg aacattttat gaatgacttt ttggatatgt tcctctgctt caaaaacagt
     1561 agggtccaaa taactttctt ttttgacaga ctccatttga tcgatcatac gattgatctg
     1621 ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga ttttattctc
     1681 taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa ccgagccatc
     1741 ttctaatgca ttcttgatgg tataggtgtg caagacctct ccatactgat catgggtcgt
     1801 acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg tacctgtaaa
     1861 gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt cagcacttaa
     1921 cgctcgatga cactcatcaa ctacaaacac gatatgttgc cccattaatt tttggaagcg
     1981 attggttcct tttttctctt cctgtttctt tgcatatttc aacgccgcat ctagtttttg
     2041 acgggttgta atgatcacaa cattcgcatt ggcatctgcc aacagagttt cacttaactc
     2101 tttggcactt cctgttccca cgatcaagct attcgctttg gcattaccag aagaaacccc
     2161 agtattaaac tccgaagcaa atttcgtaaa ctctgtggtg gtctgattat ctaaatcttt
     2221 gcggtccact aacatgatcg ttcgatcgat ccccgatttt cgtgctaaaa gcttagtaga
     2281 tacaaaacta gtcaacgtct ttcctgaacc agtcgcatgc cacacatacc ctgattgatg
     2341 tttatttgcg gcaataaaca aggcttgaat cgcatgcact tgataaggat gtaacaccat
     2401 gagggtttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca agcgatgggc
     2461 atctgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct ttttattatc
     2521 tttcgttcgc caactaaata aaaacttagg atgcaaatcc ttcggtaagg catttgcaaa
     2581 ataccgagtc gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa agatattgtt
     2641 ccgaaacatg ccttcttcag catatttttt gatttgatta aacgcctgat aaaaaccatc
     2701 ttttgcagtt acttgtttta gctcgatttg gataatcggt aagccattga tcaataaggt
     2761 gacgtcaaaa cgtcgatccc gcgcttctat gttacttcca cgttttgcaa tttgatggac
     2821 cacttcataa gtagagattc ctccaccaat gtcttgattg gaataaagga taagtgagac
     2881 agaacccaat tgcggatttt cccgttcaat cgtgatccga gccatcccat tttcaccttt
     2941 taaccatttc gcagcatcaa atggggtttt cgtccgtagc aataattcgg ttttgatcgt
     3001 ttcaaactct ttatcggtca aaggagaatc attcagttct gcttgattat ttgagatgat
     3061 tttttgacgt aaattgctcc aaagatcttc ttctgatttt agatccggac gatacgtcca
     3121 ctgattatgt ccttccccga ggacacgaat caactgttct tcgacttccg cttcctcacg
     3181 gtgactaatt ttcattcttc ttccctccct aaacaaacat cttttgcaag aagccttttt
     3241 tcgtttcctt cagcaaatct aacttacgtt gatgaagagc gatcgtatca tctagctgct
     3301 tgaagaaggc accaattttt tgttgttcat ttatatccgg tggaaactga aatgtccttt
     3361 gttctatacc acctttagtc acatgtacca ttgtcgttcc gttagttgat tctttaattt
     3421 tctctttatc agtcaatagc caagtatata gatactgttt gtccaattcc ttagataaaa
     3481 atttaagttt ccaaatatga taatggtaga ttacacgttc ttcattccac atttcaggac
     3541 cgaagcttgt tgcccataaa tataagagat ctcctttatt agcatacttg tcttcctcta
     3601 attctaagtt tgaataatac catctatcat tagtattaaa attacctact cttaaaaccc
     3661 tatattttcc agaatcaagt aattcttgtt gtttaaaagc tctaccattc aaaaaaacta
     3721 ccacttcccc caacttccgc tcttcccaat ctcccgtaaa tcctggaaaa cgaacttctg
     3781 gcacttttgc tccgttttta gggaacattt tttgtaagaa tccttttttc gtttccttca
     3841 gcaaatctaa ttcgtgctgg tgaagcatga tggtgtgatc aagttgtttg aaaaatgaac
     3901 cgatcttgat ctgttcgtca taagttggaa gtgagatagg tgtcttttta ccactatcaa
     3961 ttgtataggt tccgacggtt ccaatgcctg caacgtcacg gatatacttc tgaattttgt
     4021 tatttatgaa cgagtagtaa aagaagtttc cgtcaatttt atgctcattc ttgaaccaaa
     4081 tgatgtttcc atccttaaag taaattgggt tatcatcagt gattaataag gggacaccta
     4141 ttgatccgac accagttaca agcaagtctc cttgagaaac tttacctgaa tttttcgaat
     4201 attcattata tttttcttct gaaatgtaaa gataatcgga tggttcctca ttttttgcag
     4261 ctgagacaat atcacgagca cgtaaaaatc taatacctga gttagtccaa tctgattgat
     4321 gaattcgctt cacagaagta acatccatca tatctcctaa cttacgctct tcccaatctt
     4381 ccgtaaaacc aggaaaccgg atttctggtc gtgtatcatt gctcatgttt gaacacctcc
     4441 agcgcacctt gtaaccacgc ttcattttca ggtgttgttt gcagggaaga gatagcttct
     4501 aaaagttctt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc atttccgaca
     4561 tgaaccatat caattggttc ttcttcttcg aaagtatcga catagcgtgg gatatttaag
     4621 ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt ctcgacatct
     4681 tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgataa tttattctga
     4741 tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg agtttgacga
     4801 ttcttcttca atacaatcac cgtcgttgga atcgatgtac caaagaacaa gttcgcaggc
     4861 ataccgatca cagcataaat actgccgtct tctaataatt tttgacgaat gaccccttct
     4921 gcagcaccac ggaataaaac cccgtgtggc aagacgatag ccatagtccc tgtttccttc
     4981 aaatgataga aaccatgaag aagaaacgca aaatctgctt tggattttgg cgctaatttc
     5041 ccgtagcgat taaaccgaga atcatctaag aaggtcgtat ctgctgacca attagccgaa
     5101 taaggtggat tcatcactac tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta
     5161 ttcaacgtat ccccattgcg gagattcatt tcttcggcat ctaccccatg taaaatcaag
     5221 ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata tttaacgtta
     5281 tctggatgag ttaagtagtt acgtacattt aacatcaagg aacctgaacc catggttggg
     5341 tcaaagacac taaagaatcg tcgttctttt tgatccaacg tcacgatttg tgccatcata
     5401 tcagaaacca tatgaggcgt gtaaaattct ccggctttct tccctgcttc tgaagcaaat
     5461 tgactgatca gaaattcata cgcatccccg atcacatccc catcgtgacc taaaacttcc
     5521 acgtcattca atttcttgat aacttcggta atcgtcacat ttcgttgttg ctcatctgtt
     5581 cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg attgtaggta
     5641 gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct
     5701 tgatccgcta agacgttgaa aagatactct ggtgagatcg aataccccaa aatatcaacg
     5761 attgtggcaa tcaggtcttc ttttgattct tcatctgaaa gtaattcacg atacaacatt
     5821 gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt
     5881 tccaacaaac ggtcggataa atatttatag aaaatcagtc ccaataaata atttttatat
     5941 tctgacgcat ccattttact acggagatta tctgccgcac taaataattt ttgatttaat
     6001 tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt
     6061 ttagcatact cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca
     6121 agctacgtga cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt
     6181 ttaaccacag aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt
     6241 aagtatcata aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg
     6301 ttcactggta tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta
     6361 acaaaatggt gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt
     6421 aaaacatttg gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt
     6481 gatgatcaaa aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag
     6541 aacatgaacg gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg
     6601 atttggtgcg ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca
     6661 attcttttac aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga
     6721 gtaggaacaa caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg
     6781 gatagcccta tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg
     6841 gcagctaagt taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa
     6901 ttatggacac tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa
     6961 aattataaac aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta
     7021 taatatcttt tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt
     7081 aaaaaatcca attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt
     7141 tgaacaatta gtttaaaag
//
LOCUS       DACCVU010000136         6854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCVU010000136 DACCVU010000000
VERSION     DACCVU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.137"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            24..1178
                     /locus_tag="IVW29_002577"
     CDS             24..1178
                     /locus_tag="IVW29_002577"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7840033.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="IVW29_002578"
     CDS             complement(1200..1952)
                     /locus_tag="IVW29_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7840034.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="IVW29_002579"
     CDS             complement(2316..4982)
                     /locus_tag="IVW29_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7840035.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="IVW29_002580"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="IVW29_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7840036.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2024 a         1413 c         1248 g         2169 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaa
//
LOCUS       DACCVU010000137         6831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCVU010000137 DACCVU010000000
VERSION     DACCVU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.138"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1612)
                     /locus_tag="IVW29_002581"
     CDS             complement(59..1612)
                     /locus_tag="IVW29_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7840037.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1760..3049
                     /locus_tag="IVW29_002582"
     CDS             1760..3049
                     /locus_tag="IVW29_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7840038.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3175..4254)
                     /locus_tag="IVW29_002583"
     CDS             complement(3175..4254)
                     /locus_tag="IVW29_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7840039.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4363..4803)
                     /locus_tag="IVW29_002584"
     CDS             complement(4363..4803)
                     /locus_tag="IVW29_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7840040.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4860..5270)
                     /locus_tag="IVW29_002585"
     CDS             complement(4860..5270)
                     /locus_tag="IVW29_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7840041.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5451..5885)
                     /locus_tag="IVW29_002586"
     CDS             complement(5451..5885)
                     /locus_tag="IVW29_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7840042.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5985..6812)
                     /locus_tag="IVW29_002587"
     CDS             complement(5985..6812)
                     /locus_tag="IVW29_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7840043.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAVLG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         2442 a         1251 c         1146 g         1992 t
ORIGIN      
        1 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
       61 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
      121 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
      181 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
      241 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
      301 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
      361 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
      421 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
      481 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa cacgctatgt tcttgataaa
      541 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
      601 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
      661 atacagtaca aaaggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
      721 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
      781 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
      841 aaatatctac agagggatca ataaaaaagt caatatattc atactctgtg aattcttttt
      901 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
      961 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
     1021 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
     1081 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
     1141 ttctgccatt attagaaaag gagatagaca aaccgtacaa tgctaaaaat gcattaatct
     1201 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactt aaactgataa
     1261 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
     1321 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
     1381 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
     1441 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
     1501 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
     1561 tattgctttt tttcttcaag agaaacgttt taatcgcttc ttgagtttgc atgaaagagg
     1621 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
     1681 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
     1741 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
     1801 aagcttatcg cttactataa atcagacagt ccacacagag gagatagaaa cagccaaatg
     1861 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
     1921 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
     1981 ctattcagga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
     2041 cgctttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
     2101 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
     2161 atcagatgga ctaccgaacg atcatcatca tttaacgccg tatagtcagc ggcagacaat
     2221 tgagcaagat tcagctatcg actcaggagg agatcttgtt gaagaaccaa cagataaacc
     2281 agaaaatgaa aataagccag aagtaccgcc tacagaaaat ccagacggag aacagaaacc
     2341 ggaaatagaa cctggagaag aacccgatac agaaactcaa ccagagccag acaatgaatc
     2401 aaaacctgaa ataacccctg gagaaaaacc agatgtagac ccagaagaaa aaccggatgt
     2461 gactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
     2521 agataatgaa actgaaaatc cagaaaaacc agaggtagcc ccagaagaaa aaccagatgt
     2581 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
     2641 agataatgaa actgaaaatc cagaaaaact agaggtagat ccagaagaaa aaccagatgt
     2701 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
     2761 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
     2821 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     2881 cttaaaaagt agcaaggata caacctttgc tagtcctgaa acgaaaaata aacagctacc
     2941 aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat
     3001 agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa
     3061 tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaacgaccc caaaatacca
     3121 gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg
     3181 gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa
     3241 gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac
     3301 aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg
     3361 attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac
     3421 atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac
     3481 acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg
     3541 aaagccaaat acagccaagg cgattgtagt gagcaacgtg tttaaaagag cgataacaaa
     3601 ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc
     3661 ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt
     3721 cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt
     3781 tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa
     3841 atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg
     3901 atgctcgtaa aaatcaataa cggactggat catatcaaat gtttgatgaa taagcatcgg
     3961 gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc
     4021 taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa
     4081 ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac
     4141 aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg
     4201 ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg
     4261 atggaatatg aaaataagaa agagaaagag caaaaagaaa aaccgctaaa tgacgttatc
     4321 ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc
     4381 ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg
     4441 ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc
     4501 agcaccttga ggagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg
     4561 attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag
     4621 acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat
     4681 cacaagatac tgggtatagg taagattcag cggcttgagg agggaagtgt agtgtctaat
     4741 gatttcctta gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc
     4801 catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc
     4861 tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga
     4921 cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata
     4981 gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact
     5041 tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca
     5101 ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg
     5161 acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt atgaacttct
     5221 ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca
     5281 cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa
     5341 tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc
     5401 aaacatatac aaaaaaatat cagagacaaa aactaatctc atcataaaca ttacatatat
     5461 ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac gatggacctg atgaagcatc
     5521 cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct
     5581 gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata
     5641 tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa
     5701 ccaagagacg tgttttcatc cagcatatgg aatctcgtgc catacaaggt aaaaatgact
     5761 tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt
     5821 tgatacagtt ctagtgcttt caaactatca tttaatacaa aatctatttc cgttccttta
     5881 tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt attataccag
     5941 ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga
     6001 taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga
     6061 tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat
     6121 aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt
     6181 ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata
     6241 acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca
     6301 atagataaaa tagcataaag aaactcttct attccaaaaa caaacagatt aagagaaaca
     6361 acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa
     6421 atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact
     6481 cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt
     6541 gtttctggaa ctattgcaat gattaaagga aaaaacaagc ttccgaaaag tactttaaaa
     6601 aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa
     6661 ataactatag ctcggtcaaa tcctaaaaca gcttccagta atattcctag accgctgaca
     6721 cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag
     6781 agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt c
//
LOCUS       DACCVU010000138         6807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCVU010000138 DACCVU010000000
VERSION     DACCVU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.139"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            41..931
                     /locus_tag="IVW29_002588"
     CDS             41..931
                     /locus_tag="IVW29_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7840044.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1004..1828)
                     /gene="nadE"
                     /locus_tag="IVW29_002589"
     CDS             complement(1004..1828)
                     /gene="nadE"
                     /locus_tag="IVW29_002589"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7840045.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1840..3312)
                     /locus_tag="IVW29_002590"
     CDS             complement(1840..3312)
                     /locus_tag="IVW29_002590"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7840046.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3949..4311)
                     /locus_tag="IVW29_002591"
     CDS             complement(3949..4311)
                     /locus_tag="IVW29_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7840047.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4534..5286
                     /locus_tag="IVW29_002592"
     CDS             4534..5286
                     /locus_tag="IVW29_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7840048.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5426..6349)
                     /locus_tag="IVW29_002593"
     CDS             complement(5426..6349)
                     /locus_tag="IVW29_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7840049.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6364..6636)
                     /locus_tag="IVW29_002594"
     CDS             complement(6364..6636)
                     /locus_tag="IVW29_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840050.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2084 a         1352 c         1161 g         2210 t
ORIGIN      
        1 atgttaatat ataagaaaaa ccttttaaga ggtgatatat atgcaactac aaataaacga
       61 agaatcctta ccggtatatg aagcattagc tagtaaaaca aggatcaaga tcatccagct
      121 actttccaaa aaaaagatga atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc
      181 tatcacgacg atgcacgtga aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt
      241 aggacaacaa aaaatttcga gccttcgtgt cgataaaatc gatattagtt ttccagaaaa
      301 aatattcaat gcttttgata cgaaagaaac atcgatacct attggtcact atacaaatta
      361 tgcaattgaa ccaacatgcg gtttagctac gatccatgat tttatcggga aagtggatga
      421 accacgctat tttatggatc ctcgaaggat ggatgcccgc attctctggt ttaccagcgg
      481 atttgtcgag tatcaggcac ctaatttttt aaatacagag gatacattgg agatgttgga
      541 agtttctgtg gaaatttctt ctgaatttcc tttttcaaat gacaattggc cttcagatat
      601 cactttcagc ctaaatggtg tggagctagg aacatggacg agccctggag attttgctga
      661 tatccgcggt aaatatacgc ctgattggta tccagataat cttaatcaat acggcttgct
      721 gaagacgatc cgaattacca aacacttgac gaatatgaat ggagaaccct tgtcaaacat
      781 cacgatcaac gacatcccta aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga
      841 cgatgccaaa catgtggggg gctgtacttt atttggtaaa ggattcggta attacgatca
      901 ggatatcaaa ttaaaagttt actacagctg atatttttct tttctatttt ataaaacaaa
      961 aacagccaat ccctatgaag ggacggctgt ttttgcttta ttgttatttc cagaaatcat
     1021 ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt gttccaccat gcttcgattt
     1081 tttcttgtgc ttgagcggga acttcttttc cttctagata atcatcaatt tcattatatg
     1141 tgacacctag tgcgacttca tctgcaatca ttggtttccc atcttcaaga tctgcagtcg
     1201 gaatttttgt gtataatttt tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc
     1261 gtttgtccaa acggaataat ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga
     1321 atccagtaat gttttctgca gcatgatctg tccctaatac ggctcctgct ctttctccag
     1381 ctacggcata ttgcgtaatc atacgttgac gtgctttgat atttcctttg ttaaaatcgc
     1441 tgattcccac acctgcttcg cttaatactt gtacttgtgc atcaacagct ggtttgatat
     1501 ttactcgcag actaacatcg ggctggataa agtcaagagc agcttttgca tcttcttcat
     1561 ctgcttgttc accataagga agacgaacag cgataaattg atagttttca tcttttgttt
     1621 cttcacgcat ttcttccatc gttagttgcg ccaaacggcc tgctaaagta gaatcttggc
     1681 cgccgctgat acctagtaca aaagttttaa gaaacggata ttttttcaaa tatgctttca
     1741 taaagtcaat acttttacgt atttcttctt ttggatcaat tgttggtttg acacctaatt
     1801 cattgataat ttctgtctgc aggctcattt attctcctcc tatgcttctg tatgaagttc
     1861 tgctacgctt tttttcattc gttccatgat agccatttta tggttccagc attcagtaga
     1921 cagatcgact gggtattttt gcggattcaa gtctcgttta tactcttccc atagtgaatc
     1981 tagattttct tttgtatatt ctttgatttc gtttagtgca ggtaattcgt aaactctttt
     2041 tccttcgata aagatatctt gtaaaactgg gcgagctgta aagtctcgaa cagttttatt
     2101 gataaacgta tgcactggat ggaacatgaa gatagcttct tcttctcgag ggtcttcatc
     2161 ccataatgtc acgtagtctc cttcagattt cccatcaaaa tttcgagtga tgcgccacac
     2221 ttgttttttc cctggagtag ttactttttc tgcattgctt gataacttga tcgtatccat
     2281 catttttcct tcatcatctt cgatcgatac tagcttgaat actgcaccaa gggctggctg
     2341 atcataggct gtgatcaatt ttgtcccaac accccataca tcaattttag ctttttgcat
     2401 ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa gcatagattt ttgcttccgt
     2461 aaatccggct gcatctaatt gttcccgaac acgcttagat atataggcca tatctccact
     2521 gtcgatgcgt acgccttgga agttgatttt gtcacctaat tctttcgcca cgcgaatcgc
     2581 acttggcaca cctgatttca acgtatcata agtgtcgact aagaacacgc agtctttatg
     2641 ggttttagcg taggccataa atgcttcata atcatttcca taagattgga ctaacgaatg
     2701 agcatgggtc ccgctagctg gtatcccaaa tatcttccca gcacgtacat tgcttgttgc
     2761 atctgctccg ccgatatatg ccgctctagt tccccagact gctgcatcca gttcttgtgc
     2821 ccgtctagtt ccaaattcca atagtggatc ttctccgatg acagatttga tacgagctgc
     2881 ttttgtcgcg atcaacgttt ggaaattgac catattcaac agtgctgttt ctactaattg
     2941 gcagtgcgct aaaggccctt ccacttggat caatggttca tttgcaaaaa ctagttctcc
     3001 ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc
     3061 ttcaggatag acttctaatc cacgcaagta agcaatatcg gaatcattga acgtcagatt
     3121 ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg
     3181 catttctcga aagtaacatt caaaaacagc atgtaaatca gctcgaccta attcccagta
     3241 tgtctgcatc atattgattt gatatagatc cgtatgcagc gtcaaactgt cgtctgggta
     3301 agtattattc atcttgtatt actcctcttg gcaagttttc atttcctacc cattataaca
     3361 aaaaaaatta ataggaaaag aaatcttcca ttttttctga aaaagcacat gttttacgga
     3421 tatttcttgc taaaaatgaa ctccatctgc taaaactatc ctttcttatt ggtatttaac
     3481 ctaaacgaga aagaatactt attttattca atgtatccga gacacgaaga aattagcgtt
     3541 ttctattttt cttacttgat aaaataagta gattttccta ctacaatcgt agccgttaag
     3601 ataccgatga ttgaacaaat ataaccaaat gtctctacaa cataatcaat ggcaaaagtt
     3661 tttgaagcaa taatacgtct ttcctttttt gcttcttatc cccaaagaaa cggatataac
     3721 ttattatgta ttgaattttt ttggaaaggc gaattatgac aagatatacc ttgcgttgaa
     3781 aaaactcgcg gaaaaaatat cacacgtttt taaaaagata caaagtaaga aagcagctgt
     3841 caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc tcgccttgtt ttttattgta
     3901 tacattagat atggagcagg ttaccataaa gatgattatt agtcgttatt aaatagaatc
     3961 cttcgtattt ttttgttcta tcactacaat atctttattt ggacgtttga attgtattcc
     4021 ggcacgttct agctcctgtt tcactcctac atggactttg acatcttctc gtgtcatttt
     4081 taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg atttttttct tcgtattgat
     4141 catcacgact tgctttttat catatgtgtc taccaatagc agttgtgttc gatctaacga
     4201 aaataatttg taataattaa tagccgtatt cccaaaaaag aattccacta ctttaggata
     4261 aaatgtctta atatttaaat tactattcac aggtgtttcg attagcttca tctcattcct
     4321 cttttctata tagtcgattt agatccaatc ttaagttatg ttaccatctt tcctagcatt
     4381 aaggaatcgg ttacaattga acctttaaag acaaaaggat ttacctttta tcacttaata
     4441 gtataacata atttcatgag aaaaaaatac aattttttct tgaccatctc gttacgtaaa
     4501 ggattatgct tacatttgtc aggaggaaaa ataatggagt acacaataaa gaaaatgtca
     4561 gagatatcag gagtcagtcc gagaaccttg cgcttttatg atgagattgg actgctaaaa
     4621 ccagctagga tcaattcttc aggttatcgg atttatggca aaaaagaagt cgatcgtctg
     4681 caacacatct tattttatcg aacaatggca ttcaagttgg atgatatcca agaggtctta
     4741 gacaatccat cttttgacca ccagaaagct ttaatcaagc atcagcaaat gttgctggaa
     4801 aaacgggcac aaatcgatac cctcttgaca actgtccaac agacacttga tatgtacgaa
     4861 ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca aaagttgaaa
     4921 gaaaatgaag aatcctatgg acaagagatt cgacaaaaat acggaaaaaa aaaggttaca
     4981 gcatccaatg aaaaatggat gaatatggat aaagaaacgt atgaagaaat gcaaactgtt
     5041 gaaaagcaga tgttatttga tctcactctt tacttaaaag aaccagaaga tgaaagctta
     5101 gcagaccgta tcttccaagc tcataagaag tggttatctt acagctggcc tgaatatcag
     5161 ccaaaagcgc ataaaggact aggaatgatg tacgaagccg atgaacggtt cactgcatat
     5221 tatgacgagc gaagtggtca tggtgcagca aaagctttaa atgaaattat ccaaaaaaag
     5281 gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa taacaaaaca
     5341 gcagacaccc aagttagatt tttatgctaa cttttggtgt ctgctccatt ctttcacaag
     5401 ttttttcatc ctactttaaa tatattcatc gttgaataaa cgtataaggt attttcactt
     5461 tatccttttt ttctgtaaga aacaattgaa atgccatttc tccgcaagct tccagatggt
     5521 ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa attatcgcgt ccaatcacca
     5581 ctggtgtctt atcttgcaga tagctttgca agatgcctgc tgcaaccata tccgaatttg
     5641 taaagacacc atcaactcca agctctttaa aaaagaagcc tgcttgtttt cctttctctg
     5701 catctataca atcccaaaaa acaagtgatt catcaaaaga tggaaaaagt tgatgacata
     5761 gctgcaaagt gatttttgag ttataactta gcctgccact tcgccccaac gtgatgccca
     5821 attgctttat accttgctgc ttcaaataag taagaccttc tgtaattgat tgttctcgat
     5881 caataaagac acagcttgcc tgttttccct tgatttcttc acaaaaaact agaggtccat
     5941 atttttgata tgccaataaa tcatctagtg tattggcacg agacgtaatg atcaatccat
     6001 cgaaagcttt agctgcaaat tcttcaagat accttcgctc tttttctttc tgatagcccg
     6061 tcggtaaaag tgtcactttg taatcataat caaacgctgc ttcaatgatc ccactgatca
     6121 actgatcaaa atacggatga ttggtataag gcagaatcac tccaatgctt ttcgtttttc
     6181 cataacttaa ctgtctggca tttgcattag ggatataatc gacttctttc atgaccgcaa
     6241 gtatttcttt ccttttttcc tcatctacat atggatgtcc attgattaca cgtgaaatag
     6301 tagaaactga ataacctgtg agctttgcga tatctcgaat gtttgccact gctgcttcgc
     6361 cacctataca aaaagatttt cttgtaaata gtctaccaca gctggaccgc attcaaaaaa
     6421 ctgatacatt tttccttcat gcgaaaaact gatccgctgc ccgccccaat atatatcttt
     6481 tgtcaaattt tcgatttcat tcattggtat ttcttttact atattcctat tcaacccttg
     6541 aaaactgtac aacgttaata cggatccctt gaactcgaca aacgcataaa aataattgtt
     6601 cgttgtgcca tctgttatct ctacataagt attcattgca agccctcctt ttgatacttt
     6661 gagtatagtg tatgaaacgc gtctcatagc aaggagtttg ttttgccaaa gaaaagtttt
     6721 attcctcttt cattattacc aaagtctctt attttaaatt gaatacatta taatgatact
     6781 gtaaatatat ctttattatt agtgaat
//
LOCUS       DACCVU010000139         6727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCVU010000139 DACCVU010000000
VERSION     DACCVU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.140"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            179..952
                     /gene="sufC"
                     /locus_tag="IVW29_002595"
     CDS             179..952
                     /gene="sufC"
                     /locus_tag="IVW29_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7840051.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            965..2251
                     /gene="sufD"
                     /locus_tag="IVW29_002596"
     CDS             965..2251
                     /gene="sufD"
                     /locus_tag="IVW29_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7840052.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2248..3483
                     /locus_tag="IVW29_002597"
     CDS             2248..3483
                     /locus_tag="IVW29_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7840053.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3470..3940
                     /locus_tag="IVW29_002598"
     CDS             3470..3940
                     /locus_tag="IVW29_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7840054.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3945..5336
                     /gene="sufB"
                     /locus_tag="IVW29_002599"
     CDS             3945..5336
                     /gene="sufB"
                     /locus_tag="IVW29_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7840055.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5434..6576)
                     /locus_tag="IVW29_002600"
     CDS             complement(5434..6576)
                     /locus_tag="IVW29_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840056.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(6627..>6727)
                     /locus_tag="IVW29_002601"
     CDS             complement(6627..>6727)
                     /locus_tag="IVW29_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7840057.1"
                     /translation="ISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         2148 a         1284 c         1424 g         1871 t
ORIGIN      
        1 tgagaaataa gtctgtaatg gcttatttct catctttttt atacctattc taatttagaa
       61 atcaaaattg tttaataata aataaaagat atttgatttt agtaaggaag gtcattgcgc
      121 ttcattctca attagtctaa aatgagtgag aacgaaagag aatttggggg tataatgcat
      181 gtctgtctta gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg
      241 cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc atgggaccta acggcacagg
      301 aaaatctacc ttatctgccg ctatcatggg caatccaaac tatgaagtaa cagaaggcga
      361 aattttgttt gacgggaaaa atgttttaga cttggaagta gatgaacgtg cgcgacttgg
      421 cttattttta gctatgcaat atccaagtga gataccaggt atcacgaatg cggaattcat
      481 gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa atttctgtta tggcattttt
      541 gaaaaagtta gatgaaaaaa tggctttact aaatatgcca gaagaaatgg ctgaacgtta
      601 tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat gagatcctgc aattgttgat
      661 gttagaacct acatttgcaa ttttagacga aatcgattca ggtctggata tcgatgcttt
      721 gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac aatttcggtg cattgatcat
      781 cacccactat caacgattat tgaactatat cacacctgat gtcgttcaca tcatgatgga
      841 aggccgagta gtcatgacag ggaatgctga cctagctaaa cgtctggaag cagaaggcta
      901 cgctggtatc agtaaagaat taggaatcga ctataaagaa gaagaagcgt aagggggagc
      961 aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt tttctttaag
     1021 taaaggcgaa cctgattgga tgagaacctt ccgtcaagac gcactagcga aagcagatga
     1081 actgccattg ccgcatatcg atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa
     1141 agaaacacaa accatcagtg aaacaggcac gattccggca tttgatgcaa tgaaagacaa
     1201 tcctgtgctt gtccaacaag gttcatggac gatttttgaa caactgcctg tggaattagc
     1261 agaaaaagga gtcattttta ccgacttgtt cactgcaatg atcgaatatc cagaactagt
     1321 ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa gaccaattga ctgcactcca
     1381 tgtagctttt atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga
     1441 accactcgag tccttattca ttcaagatgg cgcatccgat gagcacttct tcaagcatgt
     1501 gttgatcgtg gcagatgaac atagtgaatt ttcttatctg gaacgtttcc agacaaccaa
     1561 agaacaagta gcaaaatcat caggtaatat catcgtggaa gtcatcgcta aagctggttc
     1621 aaaaatcaaa tattcagccg tagatcaatt aggagaaaat attacttctt atatgaatcg
     1681 ccgtgggcac attttacgag atgcttctgt cgactgggca attggtgtca tgaatgacgg
     1741 tcatgtgatc gctgactttg actcagattt agcaggagaa ggtgcacacg cagaagtcaa
     1801 aatcgtggca atcagctcgg gcagacaaat ccaaggaatc gatacacgag taaccaataa
     1861 agcccctcat acgatcgggc atattttgca acacggagtg atccgagaaa aaggaacatt
     1921 gacctttaat ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga
     1981 aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat gcgaatccta ttcttttgat
     2041 cgatgaaaac gaagtaactg ctggacatgc tgccagtgta ggacgagttg atccagaaga
     2101 aatgtactat ctgatgagta gaggattgca taaagaagaa gcagagcgct tagtgattcg
     2161 aggattcctc ggttctgtct taacagcaat ccctgtagaa caagttcgga aagaattagt
     2221 cgaagtaatc gaagggaagt tgaatggatg atcgatgcta aaaagctacg caatgatttt
     2281 cctatcttat ttcaagacgt caatgatgaa cctttagttt atctggataa tgcagcgacg
     2341 acccaaaagc caaaacaagt actccaggca ttagcgtact attacgaaca cgataacgcc
     2401 aatgttcatc gcggtgtcca tacgttagca gaacgcgcga cccatgatta tgaacaagca
     2461 agagaaaaag tacgggcgtt catccatgca aaagaaacag cagaagtgct gtttactcgt
     2521 ggtacgacga caagcttgaa ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca
     2581 ggagacgaga tagtgctctc ttacatggaa catcattcta atgttatccc gtggcagcag
     2641 ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac taacagaaga aggcttcctg
     2701 gatatggagg atgcagcgaa aaagattacg gacaaaacca aaatcgtctc cattgctcat
     2761 gtatccaatg tcttaggtgt cttcaatcct gttcaagaat tagctgaact tgtccatgaa
     2821 aacggcgggg tcatcgtagt agatggtgct caatctgccc cgcacatggc cattgatgtc
     2881 caagcactag actgtgattt ctttgctttc agcggacaca aaatgtgcgg accaacaggt
     2941 attggtgttt tatatggtaa acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt
     3001 ggcgaaatga tcgattttgt cgaattatac gacagtacat ggaaagaact accttggaaa
     3061 ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat taggccatgc aatcgattat
     3121 ttgcaaaaac tcggtatgga caatatccat cgatatgaag aagaactagc cgcttatgtg
     3181 ttgccaaaat tacaagcaat tgatggattg actgtttatg gtccacaaga cccgaatttt
     3241 cggacaggag tcgttgcatt caatttggat gggttacatc cccatgatgt cgcgacagct
     3301 ttagacatgg aaggggtagc cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag
     3361 taccttgaag tatcagcgac tgctcgcgcg agcttttatt tctacaacac aacgcaagac
     3421 gccgaccgtt tagttgaagc aatcatggca acaaaggagt ttttccaaca tgggactatc
     3481 taaattagac aatttatacc gtcaagtgat cttggatcat tcttcccatc cgcatcatcg
     3541 tggaagcttg actgactcga caagcaagat cgaattgaac aaccccacat gtggagatgt
     3601 gatcgaattg caagtcgatg taaaagacgg aatcatcaaa aatatcgcct ttgacggcag
     3661 tggctgctcc atcagtacgg caagtgcctc gatgatgaca gacgccgtca tcggaaaatc
     3721 aatagaagaa gcagaacatt taactgtaga ctttctacag cttgtccaag gaaaagaagt
     3781 caaagatccc gaccaattag gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc
     3841 tcggatcaaa tgtgcgacac ttgcctggaa agcactagga aaagcaatgg aaaacgatgg
     3901 taccgcagct ttccatcatt accacaagga agaggagtga gaacatgggc gtaccagaat
     3961 tagaagaata taaattcggg tttcacgatg atgtcgaacc ggttttcagt acaggagaag
     4021 gcttgacaga agaagtgatc cgtgagatgt caagaatcaa aggcgaacca gaatggatgc
     4081 ttgaattccg tctgaaatct ctagaaacat ttaacaaaat gccaatgcaa acatggggcc
     4141 ccgacctttc agacatcgat ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac
     4201 ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga tacatttgaa aagattggta
     4261 taccagaagc tgaacgagct tatctagccg gtgcttccgc acaatatgaa tcagaagtcg
     4321 tgtaccataa tatgaaagat gaattcgaaa agttaggaat cgtctttact gacactgact
     4381 ctgctttgaa agaataccct gaattattca aagagtactt ttcaaaactt gtaccgccaa
     4441 ccgacaacaa attagcagcg ctgaattcag ccgtatggtc aggtgggacc tttatttacg
     4501 tgccaaaagg cgtcaaagtc gatgttccat tacaaacgta cttccggatc aatgcggaaa
     4561 acacggggca attcgaacgt acgctgatca ttgttgacga aggagcaagc atccactatg
     4621 tcgaaggttg tacggcacct acttattcaa gtaacagcct gcatgccgcc atcgtagaaa
     4681 tctttacaag aaaagatgcg tattgccgtt acacaacgat ccaaaactgg tcagacaatg
     4741 tgtacaactt agtaaccaaa cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg
     4801 acggaaacct aggtgcaaaa acaacgatga aatacccaag tgtttactta gatggaaaag
     4861 gtgcacgagg caccatgctc tccatcgcct ttgcaggagc aaaccaaatc caagatacgg
     4921 gtgcaaaaat gatccacaat gcaccaaata catctagctc tatcgtctct aaatcaattt
     4981 caaaagacgg cggagaagta aactatcgag gacaagtaac ctttgccaaa aacagtgcgg
     5041 gttcgatctc tcatatcgaa tgtgacacga tcatcatgga cgaatggtcg aaatccgata
     5101 cgatcccatt caatgagatc cacaacagcc aagtctcttt agaacacgaa gccaaagtct
     5161 caaaaatctc agaagaacaa ctctactatc tgatgagccg aggcctgacc gaatctgaag
     5221 caactgaaat gatcgtcatg ggctttgttg aaccattcac caaagaactt ccaatggaat
     5281 atgcagtcga actcaatcga ttgatcagtt atgagatgga agggtctgta ggttaataac
     5341 tatttcattg ctcgaaacgt tgatattaaa gcgtttcgag cgttttttta tttgtatttt
     5401 aatggttggc tacgtttttg gctacgtttg gaattattcg cccataaatt ttgcaaatcg
     5461 gtcaccagtt ttcttaacag attgtggtgt gacatgagca tagatattca tggtagtttt
     5521 aatatcttta tgacctaatc gttcctgtac ttcttttatc gacgcaccac tttcaaacaa
     5581 taagctacag tgggtatgcc taaatccatg tggagtaatt tgaggctcca tcggtgtctt
     5641 tttgtaaatc cagtctaacc aatcgtttac tacttggggg tagtacaact tgtttgtgtt
     5701 tgtagtgaaa agaaactgtt ttggtgaatt cgtgttatag ccatataaga tcattatttt
     5761 catttgttct tgttgccaaa tacgcagttg tttaagtgta aaatcatcaa gctgaatcgt
     5821 tcttgatgaa tttttagttt taggtacttg tagaactact tgattaaact cgtcttgagc
     5881 gatagtttta ccaattgtta ttgttttatt gaaatagtct atatcttccc attgaagcgc
     5941 caatgcttcg cttttacgca ttcctgtata agctaataaa cggaaaaaag tgtacagttt
     6001 aactgaatta ttttctttag tattctgtaa aaacagttgt aattgatctt tcgtgtaaaa
     6061 cttcaagggt tgttcttctt caatttttct tggaagcaat gttttagaca taggattatc
     6121 cttacagact tccattgcta ctccaaattt aaatatttga gcagttgttt ttctcaagaa
     6181 accatattgt tttaatgttt tgtgccattc attcacctgt ttttggcaat agctaacact
     6241 tattttatcc aatcgtaatt ccccaaaagc aggtaatata tggtttttgc aaaaacgttg
     6301 agcaacggca acagaagacg gttttactct tgttctgtag ttatcaatcc ataattcata
     6361 caactcttga aaagttgtaa cattgatttt aaactcttgt ttttctttaa agtctacaag
     6421 tagtctatta agtgctgttt gcgcctcagc ttttgatttg aatcttctac gctttgtacg
     6481 tatatctttt ccagtcttgg gatctttccc taaataagtt tcaaatcccc aatatttttc
     6541 gccattttgt aatttataac taaaaaatgt tgccatttga ttctctcctt acttcattat
     6601 tgcaggtaga gaagttattt gaagaatcaa ttatttttaa atttatcgtg attatcagtt
     6661 aaaaattttg cgattatact atttttctca tatgggaaat tgtatttttt actaagatct
     6721 gaaataa
//
LOCUS       DACCVU010000140         6704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCVU010000140 DACCVU010000000
VERSION     DACCVU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.141"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            141..962
                     /locus_tag="IVW29_002602"
     CDS             141..962
                     /locus_tag="IVW29_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7840058.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            982..1542
                     /locus_tag="IVW29_002603"
     CDS             982..1542
                     /locus_tag="IVW29_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7840059.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2450..3217
                     /locus_tag="IVW29_002604"
     CDS             2450..3217
                     /locus_tag="IVW29_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7840060.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3243..3788
                     /locus_tag="IVW29_002605"
     CDS             3243..3788
                     /locus_tag="IVW29_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840061.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3789..4355
                     /locus_tag="IVW29_002606"
     CDS             3789..4355
                     /locus_tag="IVW29_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840062.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4370..4618
                     /locus_tag="IVW29_002607"
     CDS             4370..4618
                     /locus_tag="IVW29_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840063.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4637..4876
                     /locus_tag="IVW29_002608"
     CDS             4637..4876
                     /locus_tag="IVW29_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840064.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4893..5477
                     /locus_tag="IVW29_002609"
     CDS             4893..5477
                     /locus_tag="IVW29_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840065.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5584..5865
                     /locus_tag="IVW29_002610"
     CDS             5584..5865
                     /locus_tag="IVW29_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7840066.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5855..6196
                     /locus_tag="IVW29_002611"
     CDS             5855..6196
                     /locus_tag="IVW29_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7840067.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2526 a          870 c         1372 g         1936 t
ORIGIN      
        1 actttcaatt attgtgaaat atttcccttt aattaatcaa attttttata aaagtaatat
       61 ataaaataaa ttgcttgaag tattatattt agatatcata atttatggaa gaaaaattaa
      121 ttaaaaagga tgagattata atggggttac tggttccgag agctaatact tatactatac
      181 ctttaacgaa acgccaatta attgctaaaa gatatcaaag gattactaga gccatcaata
      241 gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt tcttatggga
      301 gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg atggcagaat
      361 ttgacagatt caaaaattat ttatcaaatg gaccgtctaa gttattacaa gttataaaaa
      421 acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa gttgttaaaa
      481 taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa aaagattatt
      541 tgggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac tggaaagcta
      601 caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt aataatttat
      661 tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg agttatcatc
      721 tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg aaattcgttg
      781 aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct ttattagaat
      841 actataactc acataaagtt atgggcggtt taaatttata ctctcctgga agcaatcaat
      901 ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa atagctttat
      961 aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt tcaattacgt
     1021 gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc cgataatatt
     1081 atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct tactactgga
     1141 ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc tggattatgt
     1201 tcaacaattt ctcttggatt aaacttatac tttaagaatt ttgatcttgc tgaagaagct
     1261 aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata cgtaagctta
     1321 ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga tcaattaatg
     1381 aacaaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag ctatgaatca
     1441 gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga attggataag
     1501 atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt tttatgctta
     1561 aaattttctt gtaatttttc tattcaatca ataaataatc tatatttaag aagaaatatg
     1621 atgatagaaa catctcctaa agccaccgca agccgtccta gccattgccc agtccgacat
     1681 cgctagatta gtgcatctct ccaaaaaagt tttctaaagc caccgcaagc cgtcctagcc
     1741 attgcctagt ccgacatcgc tagattagtg catctctcca taaaagtttt ctaaagccac
     1801 cgcaagccgt tctaagcata gcttagcccg acattgctag atatgctcat ctcaccatga
     1861 acgtttccta aagccaccgc aaaccgtcct agccatcgcc tagtgcctag tccgattttg
     1921 cttgatcggt tccatttctg tttatttagt gggtcaacac cgtaatctta aatagccatc
     1981 gcctatttaa gattactagg tctgctttga aggtaaaaag tctttcattc ttcaagactt
     2041 ttaggctttc aagctacgcc tgaaagcatt gaaattcatc gctctttatt tggttttgcc
     2101 atttataatt ccttacgccc attatctttt tcgcctgtca taccttgtca gaagtgttta
     2161 gagcttatct catctttgga ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt
     2221 tacgaaaata atttacttaa acacttgtat taatccggca gataagaacg cattctaatc
     2281 actcccgaca aggcaagaca ggcgaaaaag ataaataaat gaacgaaagg aagaaacata
     2341 aaatgcaaaa ccaaatagcg atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa
     2401 aaaagaattt tgaaaaacca aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag
     2461 aattttagaa gttaatcaag aaatggtaaa caagttaaca aatatcagtg ggaactatga
     2521 aaagtacgat ggtactttac ttgataatta tatttttcac gatacacaaa atatcagaat
     2581 tggcagaagt aaaccgagag cgttcattct catcaaagag aaatatttga atgaatggaa
     2641 tagcacctat gaaatggtta tgactgataa ctttgaaaag gtattggatt tcagaaatgc
     2701 tttgttgggt tctgatatgg gaaatagcag tcttgaatct attgaagttg aaaacaagac
     2761 cgagaatgaa ggtgaaggat tgattaagaa tgtcctgaaa atcgaacaaa aatatatcag
     2821 tgaagataga agtttttata gagtcacaag ctcggttggt ctagcaaact ttgctactaa
     2881 cttaattgga aacaatgcta gtgaatcttt gctagtgatg tgtctgaata caaagaatga
     2941 ggtcattgct tatagcgaag ttttcaaagg tggaattaat caatcagtag cgataccaag
     3001 agagattttt caactagctt tgctaaacaa ttcagcaaaa atcgcagtat cacataatca
     3061 tccgagcgga tatgaatttc caagtgaaaa tgacaaaagt tttactacga atttgaaaaa
     3121 cgcaggaaaa acaattggta ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac
     3181 aagctattac tcatttcaag aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca
     3241 agatgggcga aagaacacaa gcgttaatta cagtcaaata tggtaacagc aaaacgaaag
     3301 taagcgtgtc aattcattat cagtggggat atggtcgatt catgttgatg gacgttttga
     3361 atcaaggaat atatttaatc aataaagcat atgacttacg agatgaatca agagaagaat
     3421 tagcaaaagg aatctttgat agaagtacag gagttatcaa ttttgtcacc tatgaatctg
     3481 gagtgagtgc ttatgatgaa acagggtttg gtgggcgcta tgaaccttat caagaaacga
     3541 ttgaagatgt gatgaagttt tcagataaca atgatggcta tgctcattta gagatggtgt
     3601 tcgatgaaaa tggatttagt ctagatgata aaaccagatt gcgattgttt gattttgagg
     3661 gtcaacagat aggactaaac aagttttgtg aaagcggaaa agagtttgct acgaaagatt
     3721 ttattaaggg atacagatta ttgctagaaa attatggaat aaacctagaa gaagagagcg
     3781 tgaaataagt gtttgaaaaa agaaaagacc agttccctaa tttagaagcg aaaagtcaaa
     3841 aagtgattga tggtgaaatg attgaattat tccaaaaaag ttatggaacc atagaaacta
     3901 ccgcaagttg caaatactac tttcaaggaa agagaaacac gtttaaaggc tttgccgatt
     3961 ctgatgaaga attggcaaag aaaatcattg attggcttgt cagcagtgaa cacgtagcaa
     4021 gagaagatac cgttagaaca acgattgagg gtgaagtcct aacaggtact agatgtgttt
     4081 gttgtaatca tgaatttgta gaagatgaag gttgctaccg aatcaatgaa tatggtgatt
     4141 ttatttgcca gagttgccac gagatagaat ttccaaccga aagaagttgg tttgaataca
     4201 tgctacaaga ttatgagatg gaaacagaaa atggtgaagt agttttttgt accaaagagg
     4261 aattgagtaa gagaagcgat gatgaaattg aagattggtg tgtgtggtta gtagggacag
     4321 atgattgcgt tgcatattac acaacagcag aataggagtg tgaatcagca tggaaggaat
     4381 ggattttata gatcatgaag atttgattga ttttggttat acatggaaag gaatggtagg
     4441 tattagcaga agtcttgcta atgcctttta cgaaagaaat tatgcagtct atgtccttta
     4501 tgatgacaat acagaaagcc ttgttgatga agagtataaa ttggatttag agaatgtttt
     4561 atatgggata gaaaaagaag atttagcaaa atatattttt tcatggttgg gtcaataaac
     4621 gaaagagggt agcaacatga caaaattcaa aagtaaaggt atgcaatggt gttcttatta
     4681 ttatgttggg aatgatgaag agcttttgag aaaagccaaa caaaaaggtt atccagataa
     4741 gatgttaaaa cagattggct attacatgcc agaagagaga atagaaaaag aatcttgtaa
     4801 ggttcttttt gatagtgaac agctattagt tttgttaact caaaataaat tgatttgtca
     4861 atttgaaaat aattaatatg gggtgaagaa ccatgaatga gaataagatg gtattaagtg
     4921 aatacgcaag attgagaacc attaattggt tgaatggtat gcaaagtagt ggcgcttttg
     4981 atggatttga tgaggagaat atggactttg aaagtttatc gaatgaggat attagatatt
     5041 attttatcga atggtatttt acagaaccag atgaagaggt ggaagatgaa ccagaattaa
     5101 ttttagaacg tcaaagaatt ttattcagtg aacaagatag taagaggttg tatggtttat
     5161 atgacttaca actgaaagga ttaaaagcgc agtatgttgc tgatagttac gatgatatgg
     5221 tagaacaggg ggcagatagg gttgtcactt attcagaaga tgatgaagag gaatgggaaa
     5281 actattcaga tgatgagata tgctttaatg atgggtatat gcctattgaa atcaccgaag
     5341 aagaatatga taaagtgtcg gatagcgatg agattggtat gtgtacggtt gtaagtggat
     5401 tggatataga tgtccaaaga gtagaggttg cataccagaa gatgaaagaa cgtataaaaa
     5461 gtaaagtaga atcttgattt gattggattg tttgcaaagt agctaattaa gagtataata
     5521 taatcataaa tgtagttaat ggatataata atgagtatat tatactcaaa agaggtgaac
     5581 gaaatgaagg taaaaaatcc aaaagaaaaa agcagaattc aagtcggaat agacaaggat
     5641 ttaaaagaaa acgcagaaat gatattggaa gagctagggt taaatccaac aacagcaatc
     5701 acaatccttt ataagcaagt ggtcgctaga ggtgagtttc cagtagagat aaagctaagc
     5761 gaagaggaaa agcaaggaat cagattgcaa cagctaacta tggatatgcc agttgatgtt
     5821 ctagatacag atgaaaagtt agaagagtgg ttcaatgaag cctaatgaga tagtaacggt
     5881 ctatgttgca tttacagata aaaatggtgg taaaagaaga cctattttgg tggttagtga
     5941 taaagaagat agagtagaat tttttggaat cacaagtcaa tatgagaata aatcagacca
     6001 aataaagaga gtgtattttc ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc
     6061 atggattgac gtagggtctt taaaggctat tcccaaaagc cgagagaaca tatcatttaa
     6121 aaaggttggt atgctatcag ctattgatac gaaaaagcta aataggttta tcgagagatt
     6181 acaaagaaaa gtctaacaag gcttttcttt ttttgtttat atgtatatta agatatacgt
     6241 ttatataaac gtatttatgt atattaaaat acacgtaaat atatacgtga ttataaaaat
     6301 taagaaaata ttaagtattg ttaaatagtg ataattagta atatacgaat tataagatac
     6361 attttatatt taatgtatgt aaataaataa aatgactgac ggatattgaa tagtaataaa
     6421 taacaataat tcagacggat acagtgagtg aagtagcttg ctacgaacga acaggcagaa
     6481 tgaaacggta gtttcattag agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact
     6541 ggcaagttac atagagagag tttgcttgca aacgaacgtt acccacttga atagaaaaca
     6601 aaaataacat attagtgaat tttgaatagg agccacaact caaccaaccg cttgcggtgg
     6661 gtgagtccca cggaggaaaa gacgaacgaa cggtcgcgta gcga
//
LOCUS       DACCVU010000141         6644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCVU010000141 DACCVU010000000
VERSION     DACCVU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.142"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..859)
                     /locus_tag="IVW29_002612"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IVW29_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IVW29_002613"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IVW29_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7840068.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IVW29_002614"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IVW29_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IVW29_002615"
     CDS             complement(2143..2373)
                     /locus_tag="IVW29_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7840069.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IVW29_002616"
     CDS             complement(2603..3421)
                     /locus_tag="IVW29_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7840070.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IVW29_002617"
     CDS             3582..4271
                     /locus_tag="IVW29_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7840071.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IVW29_002618"
     CDS             4285..5787
                     /locus_tag="IVW29_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7840072.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IVW29_002619"
     CDS             5800..6276
                     /locus_tag="IVW29_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7840073.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IVW29_002620"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IVW29_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACCVU010000142         6633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCVU010000142 DACCVU010000000
VERSION     DACCVU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.143"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            149..2824
                     /locus_tag="IVW29_002621"
     CDS             149..2824
                     /locus_tag="IVW29_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP7840074.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3006..3245
                     /locus_tag="IVW29_002622"
     CDS             3006..3245
                     /locus_tag="IVW29_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840075.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3261..4634
                     /locus_tag="IVW29_002623"
     CDS             3261..4634
                     /locus_tag="IVW29_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840076.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4638..6272
                     /locus_tag="IVW29_002624"
     CDS             4638..6272
                     /locus_tag="IVW29_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840077.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6435..>6633
                     /locus_tag="IVW29_002625"
     CDS             6435..>6633
                     /locus_tag="IVW29_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840078.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1333 g         1907 t
ORIGIN      
        1 gttgtgattt ccaaaatcac aacttttttc ttttgtcaag attttgagtg ggatatattt
       61 ttgtagaact gaagatcatg aaattatgag aggttggagg taatgtagtc atacaaataa
      121 aaatttttta gaaagtgagt ggtaattaat gaaaatgaat cacacttcaa ttcagaagca
      181 tttacaactg ttaaatacac tggggatttc ttatgttaat ttgagaactt tttctacatt
      241 aaacaagaaa accgtttctc atattttttc tactgcagat tctcaagcaa ttattaacta
      301 tttagaaaaa cagaaattgt ctgaaggtac agtaaacata acttataatc ctattaaaca
      361 agaggtgatt acacaaaaaa agcattctgt gagagacaat gatattgaaa aaattcggtt
      421 tgtatttatt gatattgacc ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa
      481 aaaggcagaa aatgttatgg aacaagtaga acgttatttg aaagaaaaga gaatagaatc
      541 atttgtaaaa gttgatagtg ggaatggcta ccatattttt cttcccataa atgaacaacc
      601 aaataatcac gagacgattc ttactattca gaattttctt caattacttc accgaagatt
      661 tggagtagaa gatgaagttg atattgatac ttctgtttat aatccttcaa gattatgcaa
      721 attagttggt acaccagcgg tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc
      781 tagtttcctt tctattcccg ataacataga tagaagtaat tgttttaaaa ttcttgaaca
      841 gatagttgaa gaaaattcca tgatgtcaac ctctagagta aaaagccatc catcttttag
      901 agaagtgtta ccttttattc aagcagacta taaacaatgg ttagctcact atcctgaatt
      961 aacttatacc ataaaagagg gcgatcataa aggagttacc ttacttatat ttgacgaatg
     1021 tccattaaga aaacacacga ataattctaa tggttcttgt ttgtctgtta cggctgataa
     1081 taagattcga tttcattgtc tgcatgcttc ggataaaaat aaagatattc aagactttgt
     1141 aaaaaaatat ccgttgccaa aacctgtaaa tccttctgta gaatcattag aatccaatca
     1201 agaatattat tttgaccagt ttaaactgaa tcaagaagga gtatttttgg tgaaagaagg
     1261 tcaagaagaa aaagttgctt gtccgatgtt tattaagcaa atacgcaaaa atatcgaaca
     1321 aaaagtcatt acacttgttc ttgcctatta tgtcgatcat tgttgggatg aaatagaaat
     1381 agaaggtgga tgtttacaaa ctaataattt taaacaatta gcaaaacatg gcttaatcat
     1441 taaagctaga agagaagctg aagtcgttga ctttttacag attcaacggg agaatgctcc
     1501 aatctttttt gaacatgaat ctcttggctg gaaaaaagaa aatgaagcaa tgatttttca
     1561 gttacaagat tcttttggac gtgaaattcc ttctgtatta actaaaaact catgctacca
     1621 attaacaact aatggtacag aagcggaatt tgacgcattg gttcataagt atgttttagg
     1681 aacgaactta gaacttgctt ggtcgattgg tattgcttct attgtgttag gctatttaaa
     1741 cgttacgaag gctaatactt ttttgagttt actaattaac ctacagggca agagcacaac
     1801 gggtaaaacc actacgttac actttattgc ctctttattt ggaaatccaa ctacggttct
     1861 tagaaatatg aatgctacca ataaagccat tataaagcta gcatcaaaca atcagggagg
     1921 tgttccacta atactagatg agttaggagc agcaactcaa aataacttta gctcgttgat
     1981 ctatcaatta gcttcaggag aagaaaggct aagattagat tctaattgtc agcttatgcc
     2041 acaagagact ttttctgttt tgacccttac ctcttctgaa gagcgtttgg aaaattattt
     2101 caatgagaaa ttaactggtt tgaaagttcg atatatcgaa ttttctgata tcaagtttac
     2161 aaaggatgcg aatcacgcag agaaagttaa aagtctttct tctaatactt ttggttgggg
     2221 tataaagtta tttcttttga aactctttga acaagggatt acaaaaattg aagcaacttt
     2281 tgtcgaagct caaaaaaaac tatgggctgt tttacctgaa cattctttga agcaacgcat
     2341 tggtaataat ctagctttga tttatagtgg agccctccta attcaagata tactgggcta
     2401 tcctttacga ttagagcata tacagacggt gcttattaat tcgtacgaac agttaattgt
     2461 agatactagt ttaggaagtg agcaaacggt atttgaccga gtgaaaacat tgttggtgat
     2521 gaatagccat aaatttttaa caaacagtga caacaaacat acgcccagta cgatttgggg
     2581 aaaagtatca attcaaaaaa atggggatat taaggtaaat atttttccta tcccgtttga
     2641 ggacttgtta aggcgagaat ttcaagtaaa ggatctaacc tatcttttta ggcaattgct
     2701 tgattcgggt aatatgtgtg cagaaaaagg gagaagaacg aaacgtattc acatgaatcg
     2761 tcaagttctt ccaacatacg agattctatt accttcttca ctcaaatcgt attttcgatt
     2821 gtaatttaga ataaaccaaa gtatgaaagt gaggcaccat gttatggaaa tatttcttat
     2881 tgatttagca aaacaactga ttacttatct gtatgatagc gagcaaaccc aaacggaagt
     2941 aaatgaagct tgatctatgc ttcatttact tcattcaatc aaacatgaaa gaaaggaaaa
     3001 ttctaatgac taaaaataaa atgacactaa aagcagaggt tcttctgtat attcaagaac
     3061 atttttcaaa tcaagccttt tttacaaaac caatttactt ggatttcgaa ataagagggg
     3121 tatctgctgg atctatcggg ggtactcttc aagcgttgaa gaacgaagga tatttagaaa
     3181 atcactttgt gcaacgttcg ttcaatagaa gagtcgtgaa aaaatggtat ttagttcatt
     3241 catagagagg ggaatcaagc atgcgtgtgg cttgttatga tcgaacgtct gtttatgaag
     3301 aagcgaaaat tagtaatgac gctcaatatg atgcagtgaa agagtatgtt ttagagaaat
     3361 atgaacaaat tccccaacga ttttcagaca aaggagaatc aggggaaaaa gcggagcgtc
     3421 cagcttttcg acagcttaga gcttgggttt gtgcgtcaga agaagagggg gtgatctatg
     3481 tttggcgtta tgatcgattg gcacgtcaag ccaaaatagc cttagaattt attgagttgt
     3541 gccaacaaca tcaggttcaa gtgtattcga tttctgaacc tcttcctgct tctttctcac
     3601 acgcgtcaac gaataaacta tttgtgcaat tattgttcac ttttgcagaa caacaacgaa
     3661 acgtcattat tgagaatgtt cgtgccggac tttcctataa aaagcaacaa cggcaatatt
     3721 tgtcttctgc ggtaccatat gggtatcgtt tagtgaatgg gaaaatccaa caagaagtac
     3781 acgaagcaag gattgttcgc tatatttttc aactttattt gaccaaaaaa tatggctaca
     3841 aaaagctttg tcaacgattg acgcaacaaa agcttttctt ccgcgaacgt ccttttcagc
     3901 cctatcatat ttatagtatc ctaaaaaatc cattgtacta tggcgaaatc aaaggaggat
     3961 ctttggggaa gtatctagga acctttgaac cgattctctc gaaagcaacc tttttacaag
     4021 cccaagagat tcgccaatca aggtgtacag ctaaaaaaga tacgtatctg tacttattac
     4081 gtcaaaaaat tcggtgtccc ttctgtggtc gtcatctgag ttcgaagtat caatggaata
     4141 caaagaagac gaagacgctt cattattatc attgtaccga ccgaagctgc caaggaattt
     4201 ttcttcgggc agaaagggtt gagtcagaag tattggaaaa gatacaggct ttttttcaat
     4261 acgaaggcgt ctatcaagcc ttgattgccc aattaacgca acaattagcc gaaaagcgaa
     4321 aagatgaaaa acgcaaagta atccaacaaa agaaacaaga agcacaatgg attaaggcat
     4381 ttgaagaggg caattgttca ctggaagaac tcaagcaaca actgcaagct cttcaaaagc
     4441 cgatgtctac ttcttcgttt ctttcactcc aacagtgtga agcaaaagtg aaagaatggc
     4501 ttcaactaaa agacctctct agtcgacaat tattatgtga ctatgtttgt cggattgagg
     4561 taacaaagga taaaacagtt tgtgggattt acttccgaca actgacacaa aatattttag
     4621 aaaggaacga atgatctatg gaaatgatac aaaccaaacg agtcgcattg tatattcgcg
     4681 tgagtacaca agaacaagct ttagaaggac atagtattca agcacaagaa caaaatggac
     4741 gacagtttgc cgaacggatg ggctatgaag tggtagaagt ttatgccgat gaaggaatca
     4801 gtgggaaatc cacaaaaaat cggttcgcct atcaacgaat gatgaacgat gctcggaacc
     4861 atcgttttga tctactagtg atttggaaat tgacccgtct cggacgcaat atgttagatg
     4921 tcttacaaac agtcgaggaa ttattgcgat atgacgtagg attacattcg atctcggaac
     4981 aatttgacgt gaccacttcg acaggtaaat tgatgttaca actccttggg agtttcggtg
     5041 aatttgaacg caaccaaatt gcggagaatg ttcaaatgtc aatgcaaagc ttagtgaaag
     5101 ataaaaagcg ttatgcagga ggccgacgtt taggctatgt cagtggtatt gacacgaatg
     5161 gtcaaaaaca attggtcatt gaaccagaag aggcgaaaat tgttcgtctg atctatgcaa
     5221 aatatttagg cggagcaggg tatcgagcga ttgccaattt tttgaatcgt caaggatatc
     5281 aaacagtcaa aaagaatgct ttttctacca cggcagtcaa agatatctta cgcaataaaa
     5341 tttatgcagg gtatttagaa tatgcgcgct acatcaattg ggaaaccaaa cgacggaagg
     5401 ggaagaatcc gagaccgatt ttagtgaaag gaacccatga accgattatt tcggaagaag
     5461 attatcaaaa agtacaagaa cgccttgctt tagaaggaaa acaacccaaa tggaaccata
     5521 caggtgaaaa tgtattgaca ggcttgttac gttgtccaga atgtggcgca ccgatggcag
     5581 cgagtaatgt cacaaatacc ctaaaggatg gcacaaagaa acggattcga tattattctt
     5641 gttcagtctt tcggaataaa ggagcaagtg tgtgtcatgc caattcgatt cgtgccgaac
     5701 aagcggaaac atttgtcgcc gaccgattaa aggagatcgt tcagctacca gaagttcttc
     5761 cccgattggt ggcagcgtta aatgaagaaa tagtgcgaca aagccaacca ttggaacaag
     5821 aactcgtggt gttactggaa cgaaaggaag aattgaagac taaaattgaa aaatgggaag
     5881 ctgctttgga agatagtcct gaattgtttc cgatgttaaa agaccgctta gacgaattga
     5941 ctgaaaaacg acgacagttg cacattcgag aaaatgaaat cttagggatc tttcaacaag
     6001 gagagccgat tcaagtcaaa gatgtccaac ggatattgac tagtttggat cgttttcttg
     6061 cccagagtga gaagaagcaa atcaaagcct tgtatcgtac ctttatagag aaaataacct
     6121 ttgatcctaa ccataaggag atgttggaaa ttacgatgaa attcacgcat gccgtggtac
     6181 aacaattgaa tgagcaatat caaatagccg tatccaaaac aaaggatacg gctatttttg
     6241 tgttaaaaac accctttacg ttaacgatct agtctaggtt atatcaaaca acgttagagg
     6301 ggcgctaacg cttctctagc gatttgaaat cgttagagaa gtagttaggg aggtccttat
     6361 cataaatagg gatatttatt aatgaatatt tgaaatataa ttgtatataa gaattaaaaa
     6421 gagtggggat agatatgaaa aagatgggta agtgtatttt atgtgaaaaa tacactgaac
     6481 ttactaaaga acatgtacca cctaaaaaat cagggaatac tggaggtaaa aagaccagaa
     6541 ctggtaattt agatgatttc cttaagagcg attttacaaa aggtgatttt ccgaagggga
     6601 ttaaaaggaa acctcaagga aatgtttatt ata
//
LOCUS       DACCVU010000143         6629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCVU010000143 DACCVU010000000
VERSION     DACCVU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.144"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            241..2472
                     /gene="pflB"
                     /locus_tag="IVW29_002626"
     CDS             241..2472
                     /gene="pflB"
                     /locus_tag="IVW29_002626"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7840079.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2716..3477
                     /gene="pflA"
                     /locus_tag="IVW29_002627"
     CDS             2716..3477
                     /gene="pflA"
                     /locus_tag="IVW29_002627"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7840080.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3562..4488
                     /locus_tag="IVW29_002628"
     CDS             3562..4488
                     /locus_tag="IVW29_002628"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7840081.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4670..5563
                     /locus_tag="IVW29_002629"
     CDS             4670..5563
                     /locus_tag="IVW29_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7840082.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5697..5822
                     /locus_tag="IVW29_002630"
     CDS             5697..5822
                     /locus_tag="IVW29_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7840083.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5956..6588)
                     /locus_tag="IVW29_002631"
     CDS             complement(5956..6588)
                     /locus_tag="IVW29_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840084.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         2406 a         1104 c         1303 g         1816 t
ORIGIN      
        1 ttaatacagg tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa
       61 aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg
      121 taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc
      181 tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca
      241 atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac
      301 tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca
      361 gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt
      421 aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc
      481 tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa
      541 gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat
      601 acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt
      661 gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg
      721 cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat
      781 ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca
      841 atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg
      901 caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca
      961 aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt
     1021 gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg
     1081 gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa
     1141 ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct
     1201 atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag
     1261 aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg
     1321 actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct
     1381 atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct
     1441 gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc
     1501 tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat
     1561 gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac
     1621 tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc
     1681 aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg
     1741 gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat
     1801 gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa
     1861 ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac
     1921 cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat
     1981 aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt
     2041 tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca
     2101 ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca
     2161 gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct
     2221 tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga
     2281 tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt
     2341 gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca
     2401 gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac
     2461 gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa
     2521 tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg
     2581 gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa
     2641 aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa
     2701 aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc
     2761 ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt
     2821 tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca
     2881 gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt
     2941 actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa
     3001 gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa
     3061 gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac
     3121 atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt
     3181 ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta
     3241 gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc
     3301 ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg
     3361 gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc
     3421 gaaaatgcta aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc
     3481 gcatcggaaa gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga
     3541 aatcacaaag gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat
     3601 acagatgcaa tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa
     3661 acagaagcag ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt
     3721 cagctatctg caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt
     3781 gtggatcata atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct
     3841 gtagtagacc accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca
     3901 gaaccagttg gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca
     3961 attcctaaag aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc
     4021 aaatcaccaa catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct
     4081 gaagtcgatc taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat
     4141 aaatcagctg cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac
     4201 gttcgtatcg ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag
     4261 ctagaagcag caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt
     4321 gtcacaaaca tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa
     4381 gtagaagaag ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt
     4441 tcacgtaaaa aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt
     4501 atgaagcatt caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta
     4561 aaggccgaag aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt
     4621 acgtaaaagc cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta
     4681 ttcaaaaaat aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt
     4741 gaccgattat ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga
     4801 attttcgcaa aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa
     4861 gagggaagaa cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca
     4921 acaagccact tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga
     4981 acaaaaatta gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga
     5041 agcgtatttt tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg
     5101 aaatgagaag taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc
     5161 taattacttt tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc
     5221 cgaacttatt ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga
     5281 caggatacgt aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact
     5341 tgtgacgaat actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa
     5401 aaacaatttt gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc
     5461 ctttcttaaa cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc
     5521 ttatatcaaa gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa
     5581 attatactga ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg
     5641 agtattttgc ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc
     5701 aaaaaaatgg tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt
     5761 taaataatca atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt
     5821 agattaggaa gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat
     5881 aatttatcca atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt
     5941 atgaagtcgt aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca
     6001 cataaataca cctagactga cgatgaagag catatccaac agatttcctg cgacttggat
     6061 cgtcgaaggg ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat
     6121 aaaacaggtg acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag
     6181 tgaaaaaata aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa
     6241 gaaaagcgta ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa
     6301 tgcaatcaat acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc
     6361 accgaaataa acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt
     6421 ttcttcggct tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg
     6481 cgcatgtgag gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa
     6541 aaaagagcct aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa
     6601 ttaaattgat aagtatataa agtataata
//
LOCUS       DACCVU010000144         6610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCVU010000144 DACCVU010000000
VERSION     DACCVU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.145"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1495
                     /locus_tag="IVW29_002632"
     CDS             <1..1495
                     /locus_tag="IVW29_002632"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7840085.1"
                     /translation="FYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESK
                     EDLIATIVDILGYAISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDD
                     VDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKA
                     GEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQ
                     ELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWS
                     ADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGV
                     IRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQ
                     NKLSEENIQKILETYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMV
                     HVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1488..2732
                     /locus_tag="IVW29_002633"
     CDS             1488..2732
                     /locus_tag="IVW29_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7840086.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            2746..5895
                     /locus_tag="IVW29_002634"
     CDS             2746..5895
                     /locus_tag="IVW29_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7840087.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2344 a         1081 c         1362 g         1823 t
ORIGIN      
        1 tttctataaa tatttatccg accgtttgtt ggaacaagtg gttcttttag cagatgaatc
       61 cttagaggaa tatgatacgg taagcaaaca aacgatgttg tatcgcgaat tactttcaga
      121 tgaagaatca aaagaagatc tgattgcaac aatcgttgat attttggggt atgcgatttc
      181 gccagagtat cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt tccaattgaa
      241 tgatctaaac aaagcctttg tgcaattggc atctacctac aatcaattta atgggttatt
      301 tgatgatgtg gatctccaat caaaaaaact aggaacagat gagcaacaac gaaatgtgac
      361 gattaccgaa gtcattaaga aattgaatga cgtggaagtt ttaggtcacg atggggacgt
      421 gatcggggat gcttatgaat ttctgatcag tcaatttgct tcagaagcag ggaagaaagc
      481 cggagaattt tacacgcctc atatggtttc tgatatgatg gcacaaatcg tgacgttgga
      541 tcaaaaagaa cgacgattct ttagtgtttt tgatccaacc atgggttcag gttcattgat
      601 gttaaatgtg cgtaactact taactcaccc agataacgtt aaatatcatg ggcaagaatt
      661 aaatacaacg acatacaatc tagcaaaaat gaacttgatt ttacatgggg tagatgccga
      721 agaaatgaat ctccgcaatg gggatacgtt gaataaggat tggccaacag atgaacctta
      781 cacatttgat gcagtagtaa tgaacccacc ttattcggct aattggtcag cagatacgac
      841 cttcttagac gattctcggt ttaatcgtta tggaaaatta gcgccaaaat ccaaagcaga
      901 ttttgcgttt cttcttcatg gtttctatca tttgaaggaa acagggacta tggccatcgt
      961 cttgccacac ggagttttat tccgtggcgc tgcagaaggg gtcattcgtc aaaaattact
     1021 agaagacggc agtatttatg ctgtgatcgg tatgcctgcg aacttgttct tcgggacatc
     1081 gattccaacg acggtgattg tattgaaaaa gaaccgccaa actcgagacg tattattcat
     1141 tgacgctagt cgagaatttg tcaaaggaaa aaatcagaat aaattatcgg aagaaaacat
     1201 tcaaaaaatc ctcgaaacct atgctgaaag aaaagatgtc gagaaatacg ctcatttggc
     1261 aaactttgat gaaatcaaag aaaatgacta taacttaaat attccacgct atgtagatac
     1321 gtttgaagaa gaagaaccaa ttgatatggt tcatgtcgga aatgacatca agaagatacg
     1381 acaagaacag caagtgctag aaaaagaact tttagaagct atctcttccc tgcaaacaac
     1441 acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg agtaatgata
     1501 tacaaccaga aattcgtttc ccaggattta cggaagcttg ggaacagcgt aagtttgaat
     1561 gcttacttga taaaaaagat ggagtaagga ggggaccatt tgggagtgcc ttaaagaaag
     1621 aattctttgt atctaatagc aattttgttg tctatgaaca acaaaatgct atttatgata
     1681 attacgaaac aagatataaa attactgaaa aaaaatataa tgaattaatc aaatttaaat
     1741 tagaaccagg cgattttatc atgagtggcg ctggaacaat tgggagaatt tcaagagtac
     1801 caaagcaaat aaaaccgggt gtattcaatc aggctttgat tagacttaga ataaataaag
     1861 aaatcacaga ttcagaatat tttattcaat ttattcgtgc agattttatg caaagaaaac
     1921 tcacaggagc taatccaggt tcagcaatta caaatctcgt accaatgtca gaagtgaaaa
     1981 aatggatagt acaatttcca atattagaag aacaaaagaa aattgggaac ttcttcaaac
     2041 aactggacga cactatcgct cttcaacaac gtaagctaga tttattaaag gaaacgaaaa
     2101 aaggattctt acaaaaaatg ttccctaaaa acggagcaaa agtgccagaa attcggtttc
     2161 ctggatttac ggaagattgg gaacagcgta agttcaaaga gtttagtaag aagactggaa
     2221 aaaagaacac gaaagattta gacttccctg cctattcggt aagtaataaa gcaggtttaa
     2281 tttctcaaac agaacagttt gatggtagta ggttggatga tttagaaaaa acaaattata
     2341 aattcgttga accaaatgaa ttcgcctata atccagcaag agtaaacgta ggatcaattg
     2401 catttaacaa tcttggtatg acggtcattg ttagctctct ttatgtagtt gtgaaaatga
     2461 gcgaggactt agataatgag ttcatacttc agtttatcaa gtcaccaaca tttatcaaag
     2521 aggtcaagcg caacactgaa ggaagcgtac gagaatactt attctatgag aatttcgcta
     2581 atatcaagtt tccttttact aggaataaag aggaacaaca aaaaatcggc gctttcttca
     2641 aacaactcga cgacacgatc gctcttcatc aacgtaagtt agatttgctg aaggaaacga
     2701 aaaaaggctt cttacaaaag atgtttgttt agggagggaa gataaatgaa aattagtcac
     2761 cgtgaggaag cggaagtcga agaacagttg atccgtgtat tgggggaagg gcataatcag
     2821 tggacgtatc gtcccgatct aaaatcagaa gaagatcttt gggtcaattt acgccaaaaa
     2881 atcatctcaa ataatcaagc agaactgaat gattttcctt tgaccgataa agaatttgaa
     2941 acgatcaaaa cagaattgtt actacgaacg aaaaccccct ttgatgctgc gaaatggtta
     3001 aaaggtgaaa atggaatggc tcggatcacg attgaacggg aagatccgca actgggttct
     3061 gtctcgctca ttctttattc taatcaagac attggtggag gaatctctac ctatgaagtg
     3121 gtccatcaaa ttgcaaaacg tggaagtaac atagaagcac gggatcgacg ttttgacgtc
     3181 accttactga tcaatggatt accgattgtc caaatcgagc tgaaacaagt aaccgcaaaa
     3241 gatagttttt atcaggcgtt taaccaaatc aaaaaatatg cagaagaagg tatgtttcgg
     3301 aacaatatct tttcgacctt acagttgttt gtggtttcca atgaacaaac cactcggtat
     3361 tttgcaaatg ccttaccaaa ggatttgcat cctaagtttt tatttagttg gcgaacgaaa
     3421 gataataaaa aggtagaaaa cttatatgag ttttgcaaac aagtgctaaa tatcccggat
     3481 gctcatcgct tgattgcgga ttatacgatt gtcagtgaag atcaagacaa taaaatcctc
     3541 atggtgttac atccttatca agtacatgcg attcaagcct tgtttattgc tgcaaataaa
     3601 catcaatcag gatatgtgtg gcatgcgact ggttcaggaa aaacgttgac tagttttgta
     3661 tctactaagc ttttagcacg aaaatcgggg atcgatcgga cgatcatgtt agtggaccgc
     3721 aaagatttag ataatcaaac caccacagag tttacgaaat ttgcttcgga atttaatact
     3781 gggatttctt ccgggaacgc caaagcgaat agcttgatcg tgggaacagg aagtgccaaa
     3841 gagttaagtg aaactctgtt ggcagatgcc aatgcaaatg ttgtgattat tacaacccgt
     3901 caaaagctag atgcggcgtt gaaatatgca aaaaaacaag aagagaaaaa aggaaccaat
     3961 cgcttccaaa aattaatggg gcaacatatc gtgtttgtag tagatgagtg tcatcgagcg
     4021 ttaagtgctg aaaatatgga agaaatcaaa aaaatgtttc cgaaatccac ttggtttggc
     4081 tttacaggta caccgatttt ccctgagaat cggaaacaag ccaaagggca attggcacgt
     4141 acgacccatg atcagtatgg agaggtcttg catacctata ccatcaagaa tgcattagaa
     4201 gatggctcgg ttttaggatt tcaagtagag catgaaaaca ccgtggagcc gacttcatta
     4261 gagaataaaa tctatcggaa attaaaagaa gtagaaacat atgcagagta ttcaccggaa
     4321 cagatcaatc gtatgattga tcaaatggag cctgtcaaaa aagaaagtta tttggaccct
     4381 actgtttttg aagcagatga acatatccaa aaagtcattc ataaaatgtt ccgtccagac
     4441 aatgcctata caaaatttga ttttcgaaat ggccgtccaa cgaagtctgc gatcttaacc
     4501 acgagttcca ttgacatggc caaaaagtat tatcgagcga tcaaagaaat gacgaaagaa
     4561 cctgactggt taacaaaaga gtttcctaat cagcccattc gtgaaggaag aacgatggag
     4621 gatcctgatt ttccacgaat cgctattacc tattcattag aagaaaacag ccgagattca
     4681 agtgtccaac aagaagaaat gcaaaaaatt attgaggaat ataatcagta ttatgatact
     4741 tcctggtcat tagcagacat tgagcgttat aacggagaca tcaataatcg tttggcacga
     4801 aaaagagcgg aattcaaaca atttgggaaa caaattgatt tagtgatcgt agtggatcgt
     4861 ctattgacag gttttgacgc acctacgatt caaaccctat ttgtggatcg taatctagag
     4921 tatgcaggat tgatccaagc tttttcacgt acgaaccgta cgtatccaga aaaaacaaaa
     4981 ggactaattg ttactttccg aaagccagcc acaatggaga agaatgtcga ggatgcgaca
     5041 aaactgtatt cagaagcaaa agaagaatcg ggtttgattt atccaagtta tcaggaatcc
     5101 aaaaaaagat tcaagcaagc atatggtaag ttaaaagagg tttcatccat ggaatccatt
     5161 gatgaacata cgccacttga aatccgtatc gagtacgtca aagcattcca agaattgaat
     5221 cgtgcttatg aatcgttagt gacttatgac gattacaatg atgacatggt gaaatcttca
     5281 accttgaaca atcaaattca acttttggaa gaacaaattg gcgtgtacga gaccgtgaag
     5341 ggttcattaa tagaggaaga accagaaaaa gagggagaag atttttccac gatcgaattt
     5401 tatagtgaaa ataccacgaa gttgtatgat attgattcta cttatattga tcaattacta
     5461 ggaacttatg cggcgaatag ttcagatgca cgagaagaaa ttgaaaaagc tttggctaaa
     5521 ttgaataaaa cagaaggagt caaagaagtt taccgacaaa ttttaaatgc gtttgatgtg
     5581 gggactttag atagtaatga agatatcttt gtcattaaac gtcgctattt tacacaagcg
     5641 agtgatgatt tgattcatgt cttttccaat gagtggtttg tttctgagaa agaattacat
     5701 gcttctaatc tgcaatatat gccaggtgaa gatccaattc ctaatatgaa agcaattatc
     5761 aacagtaagg attacgaagg atataaagcc aaccatccag aagcgaagcc atttaaatac
     5821 gcacaagaaa tgaagcgagc atggcgtaaa atgttagatg atgagctgat tccacttgaa
     5881 aatgaattga ggtaaacgag aagagttaag aaaattgtac gattcaaaga tggtagtaaa
     5941 gtggaagtgt agaaaatagg gatagatgtc ttttatttgt tcacatttta taacgaagta
     6001 tttaagacag ctattgaata atgtttcctc cacctttttc attcatttac cccaccccgt
     6061 tgttatatga ccgtgttttg agaggaacca ataaaaagca taggctaact aggggttcct
     6121 ttttcttttg tttttttgga atccgcttaa gtgaaatgta tctgaaatga aacggagata
     6181 aagtgatttt ttgctgtaat gacaaggttt tagctgtttc agataccatg taacccaaaa
     6241 tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa atgagataaa
     6301 aagtaaatgg aatcatttca ggtaatctca aataaaaaaa tatacagtcc tgtggcactt
     6361 tacctatggg actgtatata ttttttactc tcagcgggct tgttttgact ccgtgtctga
     6421 ctccgtcgta ttgattttaa atgatattcg atgaccattt aacaggtcat ttttgaaatt
     6481 tttgtctcta tatgactcag tttgataatt attgatagat caatttgggt ctatatcgaa
     6541 caagggaata atccaataaa aaatgattta gaatatcaaa ataacaaaaa agaggactac
     6601 ctgtattagt
//
LOCUS       DACCVU010000145         6392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCVU010000145 DACCVU010000000
VERSION     DACCVU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.146"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..382
                     /locus_tag="IVW29_002635"
     CDS             <1..382
                     /locus_tag="IVW29_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP7840088.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IVW29_002636"
     CDS             complement(776..1783)
                     /locus_tag="IVW29_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7840089.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="IVW29_002637"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="IVW29_002637"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7840090.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="IVW29_002638"
     CDS             3679..4002
                     /locus_tag="IVW29_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7840091.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="IVW29_002639"
     CDS             4013..5695
                     /locus_tag="IVW29_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7840092.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6392
                     /locus_tag="IVW29_002640"
                     /pseudo
     CDS             5901..6392
                     /locus_tag="IVW29_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2034 a          981 c         1294 g         2083 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga ag
//
LOCUS       DACCVU010000146         6367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCVU010000146 DACCVU010000000
VERSION     DACCVU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.147"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVW29_002641"
     CDS             complement(99..1229)
                     /locus_tag="IVW29_002641"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7840093.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVW29_002642"
     CDS             complement(1878..3080)
                     /locus_tag="IVW29_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840094.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVW29_002643"
     CDS             complement(3140..3343)
                     /locus_tag="IVW29_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7840095.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVW29_002644"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVW29_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVW29_002645"
     CDS             4880..5692
                     /locus_tag="IVW29_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840096.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1903 a         1304 c         1004 g         2156 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaata
//
LOCUS       DACCVU010000147         6327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCVU010000147 DACCVU010000000
VERSION     DACCVU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.148"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..617)
                     /locus_tag="IVW29_002646"
     CDS             complement(42..617)
                     /locus_tag="IVW29_002646"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7840097.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(772..1298)
                     /locus_tag="IVW29_002647"
                     /pseudo
     CDS             complement(772..1298)
                     /locus_tag="IVW29_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1755..3266
                     /gene="gadC"
                     /locus_tag="IVW29_002648"
     CDS             1755..3266
                     /gene="gadC"
                     /locus_tag="IVW29_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP7840098.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3279..4679
                     /locus_tag="IVW29_002649"
     CDS             3279..4679
                     /locus_tag="IVW29_002649"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP7840099.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(4844..6279)
                     /locus_tag="IVW29_002650"
                     /pseudo
     CDS             complement(4844..6279)
                     /locus_tag="IVW29_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1950 a         1099 c         1147 g         2131 t
ORIGIN      
        1 aaaaaacgtg taatcaatta tttgattaca cgttttttat ttcaatcttc gttgtagtag
       61 tggttgcgac ataccgaaag gtaatcttca ttacctccta ctacaatctg ttcacctgaa
      121 taaactggaa taccatcaac aatacgagca ttcattacag cctttttatt gcaaaaatgg
      181 caaatagttt taatttcctc aattttatca gcaaatgtca ataatgcttc ggatcccttg
      241 aagagattat tttgaaaatc attcttcaat ccatagcaaa ttactggtac ttcctcattc
      301 attacgataa gtgatagatc aataatattc tctctactta aaaattgaga ctcatcgacc
      361 aaaatacaat caacttttcc gtactgatca ataatctcca ctaattctcc gtacacatcc
      421 atttcagaag taattgcaat agctggtttc gaaacaccta tacgactctc aacttttcca
      481 aatcctgatc gagtatctat tgcgcttgtc attatgataa ctctcttccc ttgttcttca
      541 taattatgag ccactttcag tagctcaata ctctttccac tattcattgt tccatatctg
      601 taaaataatt gtgccattaa atttattccc ttcttttaca attcaattat tccttaattc
      661 catctgcttt tatgcagctg ctcgtttatt ccttgatatg tctataacat ctactatccc
      721 taaaaccaat ttataagcca ttagagatgg tattcctgaa catctctcat attgtttttt
      781 gagtggtata tcatctgaaa aactgaagga atcatgaaaa tagcgacata cttatcattt
      841 agagttctaa ctttaaatgc atttacttct tcagtcagca ctttagtcat gttggacatg
      901 gtttggggcg tgtagtgatg cccatacatt ttctcaatca agtctgcgat ttccgacatg
      961 gtaatacctt ttcggaagag gtgaatgacg gtctcctcta atgtgtcatt cgtccgtcta
     1021 taagcaggaa cagtctgttg cttgaactcg ccattgcggt cgcgcggaat ctggagatga
     1081 agttccccat actcggtctt gaccgtacgg tcataggagc cgttacgaga attacccgtg
     1141 ttaaaaccaa tgcgatcgta cttttcgtaa tcgaggaaag ccgttaactc cgttttgaga
     1201 agcgtattga cagctttttc caaatggaca cggaataatc cattcaaatc gcctttgttc
     1261 gctagagtct ttaggatttc tgtagtaaaa tcgttcatat ggaagtcctc ttttctgtga
     1321 atgtgttgtg gtaactctat tctacagaag ggacttcctt ttttctattt acacaaaata
     1381 ttttacactc tcaacaaaag tatcttcgat taatacttgc tcaagttatg tgcagctacg
     1441 aaaaagtagg taccgaggta ctttcttttt cgcagctttt tttggactta tgagaagtac
     1501 attcgctgga agaatacgat ataaaacgag ctttttcact aaagatatta atagagtgaa
     1561 agaaacaagc ttttgttgtc agttttttca cttttctaaa aatgaaaata aaaaattctg
     1621 gaagtggaga agaattttgc ttgctatact ttgtcttgta gaaaagaggt ggacaaaata
     1681 atgcaagctc aaataagcga ttttccataa ccttggtagg aatgacaaat taaataggaa
     1741 agggaagtaa taatatgaat aaaaaaaagt tatctctatt cggttttttt gccctgacag
     1801 cttccatggt tttgactgtt tatgagtatc cgacctttgc aacatcacaa ttacatttgg
     1861 ttttctttct tcttttggga ggtattttat ggtttctacc agttgcttta tgtgctgcag
     1921 aaatggcaac agttgaagga tggaaagatg gtgggatttt cagttgggtt agtcaaactt
     1981 taggagaacg ttttggtttt gcagctattt ttttccaatg gtttcaaatt acagtaggtt
     2041 ttattacaat gatctacttc atactaggag cgctgtcgta tgtaattaat tttccagagc
     2101 ttaatacaga tccatttatc aagtttattg gtgtattagt cattttctgg gtactgactt
     2161 tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc taaagcagga tttgtcattg
     2221 gaattgttat tccttcgatc attctctttg gtttagcagc agcctacttt atcggcggta
     2281 atccgattca aatggctatt aatgcgaaag cttttattcc tgacttttct cagctttcaa
     2341 ctttagtagt atttgtatct tttatattgg cttatatggg cgtagaagct tcagcatcac
     2401 atattaatga gctggaaaat ccccaaagaa actacccatt ggcaatgttt ttactagtga
     2461 ttttggccat cgccttagat gcaatcggcg gtttctcagt agcagcggtt attccacaac
     2521 atgacttgtc tctcagtgct ggtgtaatcc aaacatttga aacgcttatt ttacatttta
     2581 acagtcattt acattggttg gtaaaagtga ttgctctcat gattgcctgt ggcgttatgg
     2641 gtgaagttag ttcttgggta gttggtccat cacgaggtat gtttgcagct gctcagcgag
     2701 gtctgttacc agttttcttc cggaaaacta ataaacaaga cgttccagtt ccgttgatta
     2761 tgatacaagg aattatcgtt actatttggg cagcagtctt aacttttggt ggaggaggca
     2821 acaatttatc attcttagta gcaatctcct taactgttgt catttatctc gtaggatatt
     2881 tactattttt cctaggttac tttgtattag tttttaagaa aaaagactta aaacgaactt
     2941 acaatgtacc aggaaaaact gttggcaaaa cgattatcgg tggaattggt tttattttat
     3001 cagtgttcgc attgcttatc tcttttgtac caccagcttc aattgcagct agtgagcacc
     3061 gaacatacca aatcatacta cttattagct ttgtagtgac tgttctttta ccttttatca
     3121 tttatgaagt acatgataaa agtgctcaca caactattga ggaacctact cacgtgaaag
     3181 ctgaagacgt taatcccgct gtttatccag ctgctagagg agagcaccac gttacaaaaa
     3241 atgatgaaca tattttaaaa cattaggagg aattcattat gttatacgga aaagataatc
     3301 aagaagaaaa aaactatttg gaaccaattt ttggctctgc aagtgaggat gttgacttgc
     3361 ctaaatataa gttaaacaaa gaatccattg aaccacgaat tgcttatcaa ttagtacaag
     3421 acgagatgtt ggatgaagga aatgcgcgat taaacttagc tactttttgt caaacgtata
     3481 tggaacctga agcagtgaaa ttgatgaccc aaacgttaga aaaaaatgca attgataaat
     3541 cagaataccc acgaacaacg gaaattgaaa accgctgtgt aaatatgatt gctgatttat
     3601 ggcatgctcc aaataatgaa aaattcatgg gaacttcaac gatcggctct tcagaagcct
     3661 gcatgctggg tggtatggcc atgaaatttg cttggcgtaa acgtgctgaa aaattaggtc
     3721 ttgatattca agcaaaaaaa cctaacctgg tgatctcttc tggttaccaa gtttgttggg
     3781 aaaaattctg tgtatattgg gatgtggaac tgagagaagt cccaatggat gaaaaacata
     3841 tgtcaattaa tctagatact gtcatggatt atgtggatga gtacacaatt ggtattgtag
     3901 gtattatggg tattacttac actggtcgtt atgatgatat caagggtctg aatgatttag
     3961 ttgaagctca caataaacaa actgactata aagtatacat tcatgttgac gctgcatcgg
     4021 gtggctttta tgcaccattt actgaacctg atctagtttg ggattttcaa ttgaaaaatg
     4081 ttatctcaat taattcttca ggtcacaaat atggtttggt atatccaggt gtgggttggg
     4141 tcttatggcg tgaccaacaa tacttaccag aagaattagt atttaaagtt agttacttag
     4201 gtggagaaat gccaactatg gctatcaact tctctcatag tgcagcacaa ctaattgggc
     4261 aatactacaa ctttgtacgc tatggctttg atggttatcg tgatattcac caaagaactc
     4321 atgatgttgc tgtttattta gccaaagaga tcgaaaaaac tggtattttt gaaatcatta
     4381 atgatggatc agaattgcca gttgtgtgct ataagctgaa agaagatccc aatcgcgaat
     4441 ggacactata tgatttatct gatcgtctgt taatgaaggg atggcaagtc ccagcctacc
     4501 cattacctaa agacttggat caattaatta ttcaacgctt agttgttcga gcagactttg
     4561 gaatgaacat ggctggtgat tatgtacaag atatgaacca agcaattgaa gagttgaata
     4621 aagctcatat tgtttatcat aaaaaacagg atgtgaaaac gtacggcttt actcactaaa
     4681 attcttcaga gaaatataaa agaagttaat taactaataa aaggagttgt gattaccaca
     4741 cagctcctta aatctatttg aataaatata tgatattcat ttgttacact ctcaatctta
     4801 gctgttgttc tattcatcat tactcttatt gtaagtagtt cagtcaaatt tgcttgttca
     4861 attgatcgat aacagtatga attgtttgaa aagtaatcgg atatataatt gttttctcac
     4921 tacctaagcc ttcttggtat aaagaactat cgcaaagtat aatccgctga gtacaaaaga
     4981 aagtgtcgtc tactatttct ctaagttgtt tacaaatgat attactaata gaaaagtgtt
     5041 ctcccaaaat gtcttctaac tgttgtcgat agtataaata cctaaagtct gacggagcta
     5101 ctatgaacac ttcaattgga gatttccgtt tatgctcatt taaaagactt aaactaatgg
     5161 ttagtttacg aatagaattc tcattccaac gcagaaataa attgttttgt tttcgccatt
     5221 gttcaagaat ttcatgaact tctaagtaca aagcgcgttg ctcttcattt aacaagtaag
     5281 tcttatcagg ttgaaatatc tgaatatcag cccaaagtga gcggataaac gggagaagtg
     5341 ctttatcaaa aagtatctga ttttcttcag atatattcag agataagata agtttttgac
     5401 tgagctcaat caccaaaggg ttatctgcta catggttttg ataaacaacg ttcaagtctt
     5461 tttgtaacaa ttcaaagtta tcgcatagat aatttctaga gttgaataga aagtagatat
     5521 aaaggcattc atcttcgtca tgacataaag agaaagaaag ttgctctaat cctttctgta
     5581 ttagctgaaa caagggagta tgtcgggcaa ctcttttttc ttcctgacta aaatatattg
     5641 ggttagtctt agatctctgc aaaccgatag cgatacctaa taaaataaat tgtttttcaa
     5701 ctgtagggta acttcttgaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct
     5761 ttgaataatt gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt
     5821 tagtaacaaa cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta
     5881 ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga
     5941 aatgaaggta gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag
     6001 tacattgaaa aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga
     6061 agagtcgaat gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg
     6121 gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagtg agtgggcaaa cgttcaattg
     6181 atctgctatt tcgcgaatct gaatggtccc tttttcgtgt aattgctcac aaatgtaaac
     6241 ttgacgaaaa atacttttct ccagataggt ttccaacata gtactcctcc tagtcatttt
     6301 atacataact attataactc taccaat
//
LOCUS       DACCVU010000148         6216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCVU010000148 DACCVU010000000
VERSION     DACCVU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.149"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            667..891
                     /locus_tag="IVW29_002651"
     CDS             667..891
                     /locus_tag="IVW29_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7840100.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            911..1198
                     /locus_tag="IVW29_002652"
     CDS             911..1198
                     /locus_tag="IVW29_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7840101.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            1648..2028
                     /locus_tag="IVW29_002653"
     CDS             1648..2028
                     /locus_tag="IVW29_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7840102.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2010..2924
                     /locus_tag="IVW29_002654"
     CDS             2010..2924
                     /locus_tag="IVW29_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7840103.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            3169..3858
                     /locus_tag="IVW29_002655"
     CDS             3169..3858
                     /locus_tag="IVW29_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840104.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3979..4065)
                     /locus_tag="IVW29_002656"
     CDS             complement(3979..4065)
                     /locus_tag="IVW29_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7840105.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4741..5691
                     /locus_tag="IVW29_002657"
     CDS             4741..5691
                     /locus_tag="IVW29_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7840106.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            5712..>6216
                     /locus_tag="IVW29_002658"
     CDS             5712..>6216
                     /locus_tag="IVW29_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7840107.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDT"
BASE COUNT         2330 a         1074 c         1150 g         1662 t
ORIGIN      
        1 aaaaaaaatg gtggcaacta tggaaataac tggagggaac attcatgaca agaaaacaac
       61 ttctattcgg ttcgatttta ctaggaattc tcggtttcag tccgatgtta ttgtcagtga
      121 tcaataaagg gaataaacgt ggtgaattaa aattgaatcc aaattattac aacagtccta
      181 acgaagaatt gtttgtaaaa gaaacggata aataataaaa aagactgctc ttccgagcag
      241 ccagttaaaa aaataatgag agtttattct tgaaaaaagc aacgcgctta taaagcgcta
      301 ccaaaaattt atcatatttt atgaaaaata acaccccagt aaaaaatgac tggtaaaaat
      361 tcacaaagaa acggaaagaa gcaactgcag aaaatcaagc cttaaaaaga gctttttacc
      421 tagaccaatc caaaatcaca aagtgagccc ctgtatcggt tgatacaagg gctacttggc
      481 atactacaca ttatgtctac tagctgttta aataactgat cttataattt tatttaaacg
      541 attatgaatt caaaactact ttttatgacg ttaatcattg ttatataata aaatgttata
      601 taataaaatt actaagatta tttaatataa caagcaattt taaaaaaacg aaaaggaaaa
      661 acaatcatga aaaagaaagt attaaaacat tgtgttattc taggaatatt aggaacttgt
      721 ctagctggca tcggtacagg aataaaagtt gatgcagcta cttactatgg aaatggtctt
      781 tattgtaaca aagaaaaatg ttgggtagat tggaatcaag ctaaaggaga aattggaaaa
      841 attattgtta atggttgggt taatcatggt ccatgggcac ctagaaggta gtggtaggga
      901 ggttattata atggatttta ctaaagaaga aaaactttta aatgcaatta gtaaagtata
      961 caatgaagca actatagatg actatcctga cttaaaagaa aagctctttc tttattctaa
     1021 agaaatcagt gagggaaaaa gtgttggtga agttagtatg aaattaagta gttttcttgg
     1081 aagatatatt ttaaaacata aatttggatt acctaaatct ttaatagaat tacaagaaat
     1141 tgttagtaag gaatctcaag tatatagagg atgggcttct attggtattt ggagttaatt
     1201 ctttggaata aaatgaaaat gggactgaag aatcaattct gattcatcag tcccataaat
     1261 tatagagccg aaaaattatc atatttatag atttattaat agtaaatggc ataagaatct
     1321 ttgaacaacg aaaaaaatgt agctctacct aattattgat ttgcaacaat ttcaccgaag
     1381 gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagtctttt ttgtattgtg
     1441 gagagtgggg gggaggggga ttcaaggggg agcttgcgcc cctttgacaa gcggttattt
     1501 gcaccaaata cgtaggtatt tggctagcaa atatagagct tgccaagccc tttagtgagc
     1561 tttagtgagc tttacttatt gtaccttttt aggtacaatg gtattgcgaa cgatacgtcc
     1621 gtaggacgta ctgaaagggt gggacttatg agcgaacaaa atcaatattt ggctagcaat
     1681 cattctaaaa aatataccta tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa
     1741 tccgaatttg caaagctgaa gcagtcagct gaagctttgc aaatgagtgt gccggctttc
     1801 gtgaaagcca aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgct
     1861 caggcaatcg cgcgcgattt agccaaagct ggcgggaata tcaatcaaat tgccaagtgg
     1921 tgcaacacac accaacacga cgttgatcct ggcgacgccc aacgcttatc cgaaaaccta
     1981 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagc cgaaccactt
     2041 cttgtagccg agcgatcaat tacgccgaac cacgagcaac cgtcaaaaca gggataaact
     2101 gtgacattga ttatgccaaa agcgaaatga aacaaattcg catgctgtat ggaaaagacg
     2161 atcgtgtaca agcacactta ttgatccagt cttttcgacc aggagaaatt accgctgaaa
     2221 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctgaccac caaatcgcga
     2281 tttacacaca tacagacaaa gaccatattc ataaccacat tgtcattaac tccgtcaatc
     2341 tggaaactgg gaacaaattt caagcccatg gtcaagcatt tttaaacaaa tgttatgacc
     2401 taaatgacga gatttgtctt gaacatgact taacaatcac tgaacgaggg aagaaacccg
     2461 aaaaacgaac catgtctgaa atcaaattaa aagaaaaaaa tgaacctgtt tggaaagacg
     2521 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tataaccaat
     2581 tttgtgattc tcttaaaaca ttcggcattc attgcttgaa tcgtgggaaa aattttactt
     2641 acgaattgat ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
     2701 aggagacgat tttacatgag ctggacagac gagaacgagc ctacaatcaa caacgaacca
     2761 acgagctacg aagagctcga gctagcattc gcgaaactgc aaaagaagta cgaagagaac
     2821 aacgaacttt tacgcctgaa caatcggaaa ttagaccgac tcgaagaaac catgaacaac
     2881 acgttgaacg aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacatc
     2941 gaaaacgaaa taaagagaca acgtgccttt cttgcgcctt atacccaaga aaggatacaa
     3001 actacgatgg attcacaagt caaaaaactc caccaagcgc tattagacca gcgaaaccaa
     3061 gaacaaaaaa aggctttaat tcaacgatcg atggatattc ttgccccctt atgttgctta
     3121 gtactaacat tattagcctt ttggttggta tggggagaat aaacagacat ggcattctat
     3181 ttaccctatc tactcatttc tgccagtatt ttaggaatta tttggcttag ctataaggct
     3241 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttattggtc
     3301 ggggttatat ttgccttatc aatcgtttta agtggtttat tcggcgtttt agagggtaat
     3361 aaacgtgttt caagaagcat tttattaggg gatatcactc aaaagtatga agcagcatgt
     3421 gataaaaaga aaaaagagca agcattagca caaaaaatgg aggaatttac cgcttgttat
     3481 gaggacatga acaatatttt tgtggatcaa gagaaacgtt taacagataa aaacatgaaa
     3541 aaactcactc gtttttatca aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa
     3601 tatgagcaag taaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     3661 tgtaacttat ttagcgatac aatccctttt tcaaccagtg aacaagaacg aaaagaacgg
     3721 caacaatcgg tcacatacga acgttatgaa acactgcttc agcaagcaac aaacatacaa
     3781 aatccaacga aaaaagaaac ggcattaaat tatctagaaa gtgtcaagga atggttggat
     3841 cagcaacaac aaaattgaat ttgattagca atcatgttaa actgaacctg caaatgatca
     3901 aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt tttcaggttg
     3961 atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac tgaataaaga
     4021 gacacaaact tctacaagaa gggggcaaat gaaccgttca aacattagat acacctcctt
     4081 ccagtcgcaa gactgtcgga taggcgacaa gttattatac cttacaaaat aaaatctgtg
     4141 tggtgttttt aagtatccct tattttcggg ttgttagggc ttgccctgac cgcctgtaag
     4201 gcgctttaag aacaagataa ggttttttac ccaagactgt cttaaaacga cttagacgag
     4261 ctatcagagg cttcccccac ctccgttact gacttgtttt tagcaaagga gcctttaggc
     4321 gactgcgcag tacaatgtga gagcatagcg atcacacttt tttagcgacc gtagggagca
     4381 aggttttgaa ttggcagttt tgaaaaatgc gcgaacctgt tggtagaaaa aagaagtggt
     4441 ttttaggcgg gaaattttgt caccctcttt tataatctct ttttaaacct ttttttaaac
     4501 ctctttaccg tcggctgtgt ccttactcct tcaatggata cagcgtttta taaactacat
     4561 tttgtctgct tttaaactac attttgtctg cttttaaact acattttgtc tgcttttaaa
     4621 ctacattttg tctgctttta aactcttttt ttgcactcac gtatgttata atatgtatta
     4681 tcacagacaa aacgttgttt ataacgtctg tatccattga aggagtaagg aggaatatct
     4741 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt
     4801 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat
     4861 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct
     4921 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt
     4981 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa
     5041 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct
     5101 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa
     5161 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct
     5221 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac
     5281 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
     5341 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
     5401 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
     5461 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
     5521 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
     5581 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
     5641 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
     5701 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
     5761 agatgagtag gcaagcaata tcaaaacata tccaaaaatt agattcttcc atgatagcta
     5821 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
     5881 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
     5941 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
     6001 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
     6061 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
     6121 aacaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
     6181 aagcaacaga aacaatagac aaacaaattg atactg
//
LOCUS       DACCVU010000149         6108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCVU010000149 DACCVU010000000
VERSION     DACCVU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.150"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(320..805)
                     /locus_tag="IVW29_002659"
     CDS             complement(320..805)
                     /locus_tag="IVW29_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840108.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(944..1159)
                     /locus_tag="IVW29_002660"
     CDS             complement(944..1159)
                     /locus_tag="IVW29_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7840109.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1383..2183
                     /locus_tag="IVW29_002661"
     CDS             1383..2183
                     /locus_tag="IVW29_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7840110.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2202..4070
                     /locus_tag="IVW29_002662"
     CDS             2202..4070
                     /locus_tag="IVW29_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7840111.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4063..4554
                     /locus_tag="IVW29_002663"
     CDS             4063..4554
                     /locus_tag="IVW29_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7840112.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4542..5096
                     /gene="srlA"
                     /locus_tag="IVW29_002664"
     CDS             4542..5096
                     /gene="srlA"
                     /locus_tag="IVW29_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7840113.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5114..>6108
                     /locus_tag="IVW29_002665"
     CDS             5114..>6108
                     /locus_tag="IVW29_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7840114.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2161 a          968 c         1224 g         1755 t
ORIGIN      
        1 tgaaaaaggg atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt
       61 cttctatcca aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa
      121 ctcagttgac cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt
      181 gagtatattc ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt
      241 atactccgta taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa
      301 tccgtataat atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc
      361 cttcgggtga cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt
      421 tgctggaagc tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat
      481 cgtcactttg taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt
      541 ctaatggaag agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg
      601 actgaatact tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg
      661 aagtagtaga agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt
      721 ctttaacaga attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca
      781 agcctgcgta aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag
      841 tatatcactt aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg
      901 gaaggttcat gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa
      961 cttttctagt aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga
     1021 tataaatgat cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa
     1081 gttcgaacaa gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa
     1141 tagcatctaa agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca
     1201 aaaagagaaa cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga
     1261 aaattttttt tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc
     1321 ttaatttttt ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac
     1381 atatgtcgga ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag
     1441 gaattggaag aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg
     1501 atgtaactga atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt
     1561 ctaaaacgga catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg
     1621 gtctagtcaa taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa
     1681 aaagcaagta tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa
     1741 gtgtatattt gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga
     1801 tcgtgaatct ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg
     1861 ctacaaaagc agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga
     1921 atgttcgtgt ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag
     1981 aatatgaaga agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt
     2041 acagtcgaac aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc
     2101 tagtctgcta ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt
     2161 caggaggtaa aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat
     2221 ggtaccaatt attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa
     2281 aagaaatcaa aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa
     2341 ttgccggtat tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt
     2401 ttgatttttt tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg
     2461 catccaaaag aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta
     2521 tggatgattt gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga
     2581 cattaaaaga gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac
     2641 taagaataat aggagatgag ctagaattac gactactttt attttatttt gtttctccct
     2701 acttttcaga gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc
     2761 agaagaagac gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt
     2821 ctttgaatag aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt
     2881 gttttgaata tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac
     2941 taactttgag ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa
     3001 gtataacgat gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga
     3061 tgcgtattcg acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga
     3121 aacagcacct ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat
     3181 tttatggtga gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac
     3241 aagcattaga agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt
     3301 tgtatttgga gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca
     3361 ttgttcatac gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat
     3421 tggggaggga tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa
     3481 acaaatattt cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg
     3541 tgatccattt gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa
     3601 aaaaaaagcg attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa
     3661 aaaagacgta tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg
     3721 tggatgaaga atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta
     3781 acaaagaatt tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag
     3841 tagggctaat aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat
     3901 ttgccatacc tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata
     3961 tcctatttga actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag
     4021 aatcattttt aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta
     4081 ttcatagttt tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta
     4141 aagcatttca atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa
     4201 cgacctggga aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa
     4261 gttttagaag tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct
     4321 gtctatgcgg gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa
     4381 gatcgtttga cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca
     4441 ggaaatgaaa aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg
     4501 atatggaaaa gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat
     4561 ttgctgaggg atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga
     4621 caggaatcgt accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt
     4681 taggtgaaga ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt
     4741 accttgtgtt accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg
     4801 ctcgtttcct tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc
     4861 atacgagcaa tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga
     4921 ttgctcaagg gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc
     4981 ttgtgggact gctgatgaac tttattggtg gttggataac tgattttact actggatttg
     5041 tatgcagaca gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa
     5101 aggagagttt taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg
     5161 tcctttgatt attaggccga caaaagataa acacaagttc atctatgtca caggtggagg
     5221 tgagaggcca atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg
     5281 gttcaaaaca tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac
     5341 tcttagatgt ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg
     5401 aaaaagtggc ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt
     5461 agaacaaata cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc
     5521 tccagataaa aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca
     5581 gcaaaactgg atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca
     5641 atcagcaaga gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt
     5701 ttcccttttg attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat
     5761 ggcaccttta gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc
     5821 atttttatct cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat
     5881 cggtgtagag attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc
     5941 tatcaatact caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa
     6001 agctgagaca gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat
     6061 cccacgagtt atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACCVU010000150         5946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCVU010000150 DACCVU010000000
VERSION     DACCVU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.152"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVW29_002666"
     CDS             <1..813
                     /locus_tag="IVW29_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7840115.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVW29_002667"
     CDS             807..1229
                     /locus_tag="IVW29_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840116.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="IVW29_002668"
     CDS             2399..3367
                     /locus_tag="IVW29_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7840117.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="IVW29_002669"
     CDS             3384..3908
                     /locus_tag="IVW29_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7840118.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="IVW29_002670"
     CDS             4284..4565
                     /locus_tag="IVW29_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840119.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="IVW29_002671"
     CDS             4567..4983
                     /locus_tag="IVW29_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840120.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5605..>5946
                     /locus_tag="IVW29_002672"
     CDS             5605..>5946
                     /locus_tag="IVW29_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7840121.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2256 a         1016 c          963 g         1711 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttgag gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggcatagtgt acattatgtt aactaacgct ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat
     5581 accacttaaa tttaacacaa aggcgtgatt attttgaaac ccaaaaagaa aatttatcta
     5641 ggtctcctct tttccttgat gattggaatt tttagtatag gaattacaaa tacctatgct
     5701 tcagaaaatt ctaatttcaa tatcaactcc caaccaattg aagaacaagc aattaatttt
     5761 gcgaaaactg attttgaaca ctccataact tcaattcttt taatggaaaa taacaccgaa
     5821 actgatatta actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca
     5881 aatagttcta ttttctttcc agtattttca gacgctgata atacgattaa atatatttat
     5941 accgtc
//
LOCUS       DACCVU010000151         5897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCVU010000151 DACCVU010000000
VERSION     DACCVU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.153"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(123..581)
                     /locus_tag="IVW29_002673"
     CDS             complement(123..581)
                     /locus_tag="IVW29_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7840122.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(594..1919)
                     /locus_tag="IVW29_002674"
     CDS             complement(594..1919)
                     /locus_tag="IVW29_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7840123.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1936..3348)
                     /locus_tag="IVW29_002675"
     CDS             complement(1936..3348)
                     /locus_tag="IVW29_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7840124.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3365..4129)
                     /locus_tag="IVW29_002676"
     CDS             complement(3365..4129)
                     /locus_tag="IVW29_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7840125.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4233..5108)
                     /locus_tag="IVW29_002677"
     CDS             complement(4233..5108)
                     /locus_tag="IVW29_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7840126.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5363..5447)
                     /locus_tag="IVW29_002678"
     tRNA            complement(5363..5447)
                     /locus_tag="IVW29_002678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5411..5413),aa:Leu,seq:caa)
     gene            complement(5465..5536)
                     /locus_tag="IVW29_002679"
     tRNA            complement(5465..5536)
                     /locus_tag="IVW29_002679"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5502..5504),aa:Gln,seq:ttg)
     gene            complement(5546..5618)
                     /locus_tag="IVW29_002680"
     tRNA            complement(5546..5618)
                     /locus_tag="IVW29_002680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5583..5585),aa:His,seq:gtg)
     gene            complement(5680..5764)
                     /locus_tag="IVW29_002681"
     tRNA            complement(5680..5764)
                     /locus_tag="IVW29_002681"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5728..5730),aa:Leu,seq:caa)
     gene            complement(5785..5855)
                     /locus_tag="IVW29_002682"
     tRNA            complement(5785..5855)
                     /locus_tag="IVW29_002682"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5821..5823),aa:Cys,seq:gca)
BASE COUNT         1806 a         1254 c          896 g         1941 t
ORIGIN      
        1 aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc tagtgattga aacatatcat
       61 ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt tttcatcgta
      121 cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt
      181 ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac
      241 ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc
      301 cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt
      361 tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt
      421 attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct
      481 atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta
      541 gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt
      601 aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact
      661 ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct
      721 tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat caatccttta
      781 tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc accaacatat
      841 gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg
      901 attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat
      961 tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta
     1021 tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac
     1081 tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc
     1141 gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa
     1201 gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata
     1261 atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag
     1321 tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca
     1381 atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac attcggatat
     1441 ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct
     1501 ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc
     1561 ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga
     1621 atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa
     1681 tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa
     1741 ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca
     1801 atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga
     1861 acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc
     1921 atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac
     1981 ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc
     2041 taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg
     2101 tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt
     2161 tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac
     2221 ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat
     2281 caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa
     2341 aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt
     2401 aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc
     2461 atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat
     2521 atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt
     2581 ttctttataa gctgctttat agatagctag acatatagct ggtataccaa acatcattac
     2641 tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat
     2701 ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata
     2761 cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat
     2821 ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg
     2881 gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat
     2941 agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt
     3001 aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat
     3061 attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc
     3121 agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc
     3181 aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt
     3241 cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg
     3301 aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc
     3361 tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc actttttgag
     3421 caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac
     3481 catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt
     3541 ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt
     3601 ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa
     3661 cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa
     3721 caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg
     3781 gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat
     3841 ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga
     3901 taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta
     3961 cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg
     4021 ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca
     4081 caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct
     4141 tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt
     4201 tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc
     4261 aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta
     4321 atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata
     4381 ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc
     4441 attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga
     4501 tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct
     4561 cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg
     4621 gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga
     4681 taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt
     4741 tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag
     4801 gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga
     4861 atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat
     4921 ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc
     4981 cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt
     5041 ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac
     5101 tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag
     5161 aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag
     5221 tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga taatacttgt
     5281 atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac tctctataag
     5341 aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg
     5401 acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata
     5461 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     5521 gcttggctat accccaagaa taaaaggcga atgatgggaa tcgaacccac gaatgccgga
     5581 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
     5641 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
     5701 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
     5761 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
     5821 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
     5881 taaagaataa attatag
//
LOCUS       DACCVU010000152         5795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCVU010000152 DACCVU010000000
VERSION     DACCVU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.154"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            114..1088
                     /locus_tag="IVW29_002683"
     CDS             114..1088
                     /locus_tag="IVW29_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7840127.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1070..1531
                     /locus_tag="IVW29_002684"
     CDS             1070..1531
                     /locus_tag="IVW29_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7840128.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1581..2045
                     /locus_tag="IVW29_002685"
     CDS             1581..2045
                     /locus_tag="IVW29_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7840129.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2059..2808
                     /locus_tag="IVW29_002686"
     CDS             2059..2808
                     /locus_tag="IVW29_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7840130.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2808..3638
                     /locus_tag="IVW29_002687"
     CDS             2808..3638
                     /locus_tag="IVW29_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7840131.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3638..4285
                     /locus_tag="IVW29_002688"
     CDS             3638..4285
                     /locus_tag="IVW29_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7840132.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4383..5159)
                     /locus_tag="IVW29_002689"
     CDS             complement(4383..5159)
                     /locus_tag="IVW29_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840133.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5303..>5795
                     /locus_tag="IVW29_002690"
     CDS             5303..>5795
                     /locus_tag="IVW29_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7840134.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLE"
BASE COUNT         1981 a          823 c         1046 g         1945 t
ORIGIN      
        1 ttataaaaaa atttttttga aaaatagtat tgcaaaataa agcaatttca tagataatag
       61 gtattgtaaa gttatatgac tttataatac ttattagagt gagggataaa gaaatgagac
      121 aactacacaa agaacagatt gaaacagcaa tggcagcgtt aaaggaaaag acaattgatc
      181 atgtatattt tacagcatgt gggggttcac aagccgtttt aatggctggt caatatctat
      241 ttgataaaga attaactgta ccttgtcatg tttatactgc taacgaattt aactatgcta
      301 taccaaaagg attatctgaa aattctttag ttatcacttg ttcccattct ggcacaacac
      361 cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg agcaatgact atttgtttat
      421 ctaatgaaga aggatctcct ctatgggaag cagctgaata tcctgttcac tatgattggg
      481 gtaaagatgt tgatgctagt gatttaaata aaggaattct ttatggttta ttatttaata
      541 ttttacttgt tttagatggc tctaaaaaat gggaaacttg cttgaatgaa tttgataagt
      601 taaatgatct tgttattgct gaaaagagca aatttgaaat aactgctaaa gaatggggta
      661 aaaaccaaaa acgagataaa attatttaca caattggtag cggtatcaat tacggagaaa
      721 tttactccac agcagcttgc tggttcatgg aaatgcaatg gattaattct aatgctattc
      781 attctgggga atttttccat ggtccttttg aaatcactga ttatgatgta ccattcatct
      841 tagtgaaaag tattggagat acaagatttt tagatcaacg ggtagaagac ttcactaaaa
      901 aattcagtaa taaattaatg gttttagacc aagctgattt tgacttagaa tctatagata
      961 gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt tgttattcgc caaatggtag
     1021 aagcgattgc ttttgaaaga ggccattcct tagatgttag acgctatatg tggcaaatga
     1081 aatattaatt aataggaggt tgcaaaagat ggcaaatcaa ttatttcaca ttgtgattac
     1141 aacacattct accctttgtg agggttattt aaaggcaaca aaattaattt taatgaatga
     1201 cgccacaggt attagtgctg ttccatttaa agaaaatact agtccagatg attttgagga
     1261 gagtatgaaa gaaataatta gtcagcatca tttgcaacct ctaattattt taacggattt
     1321 aattggagga acacctaata atgtttctat taaacagttg aacaacaata gaattcaaat
     1381 tattgctggg attaatttac ctcttttact ggagttatta atggcccagt cagctggtaa
     1441 atctatagaa gaaatagatt ttaaggaatt actgaataac tctcaaaaaa gtatggttca
     1501 tattaattcg cttttaaaag atgtcagtta aggtggcaat ttctattcta taaattttaa
     1561 aaattgaagg aggaaataga atgattaaat tagtgcgtgt agaccacaga ttacttcatg
     1621 gacaagtaat attttcttgg actaaacagt atgatattaa ccatattatt gtggctgata
     1681 acaaagttcc aggagatcca atgtctgtga tggctttgac aattgctaaa cctgctgatt
     1741 gcaaattaga cattgttaaa attagtgatg ttaaagggat tatggaaaca tccccaaatg
     1801 ataattacat gattttaatt aaaggaccta aggaagcttt tgaattagtt caaaatttac
     1861 cagaaattaa agaaattaat tttggcggtg tgtctaagaa aagcgagtcc aaacaatatg
     1921 gaaaggcagt ctttttaact cctgaagaat tagaagccac aaaaaaaatt cttgatcaag
     1981 aagtgaaaat atttgtgcaa caagtcccat cttcaagtat tgaaaatgta gattttagta
     2041 agtaaggggg gaaaataaat gttattaaaa gccattttat taggtttagt tggtgtggta
     2101 gctgttattg actccaggtt aattggtcga caaaatattg gtagaccact aattttgagt
     2161 acacttgccg gacttgtgct aggtgacttg catacaggga ttgttcttgg ggcatcttta
     2221 gaattaattt ctatgggatt cgtctcaatt ggggcggctg gacctcctaa tatgcaactt
     2281 ggtagtatta ttgccactgc ttttgcaatt ttaactaata gtagtacaga aacagcctta
     2341 acaatagcta tcccagtggc tgtagctggt gaatttatga gtattatcat gcgcatgata
     2401 attgctcaat ttggacatgc tgcagataaa gccattgaaa ataataaatt tagaaaagct
     2461 caaattattc atatttattg gtcatttatt tttaatgctt ttgtttactt tattccaatc
     2521 tttttaacag tatattttgg tgctgacgtt gtggcagatt tagtagataa aattccagaa
     2581 atgattacag acgccttgac tgtctctgga aatatgcttt ccgcattagg gtttgcaatg
     2641 ctattaagca caatgttgag taaaaaatta tatccttact ttatttttgg attctttatt
     2701 gttgcctatt caggtctttc actaattggc gtgacaattt ttgcagctct gattgccttt
     2761 gtaatggatc aagttaaata tggaaaaaga gaagaggcgc atggttaatg gaaaatagta
     2821 cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa gatgtgtctt cgttcatgga
     2881 caatcatgtg ttcgtggaat tatgaaagac aaatgcatat gggatttatg tatgggatgg
     2941 cgccaatttt aaataaagtt tataaaaatg atgaagagaa aaaaagagaa gcttatactc
     3001 gtcatatggc attttttaac tgtacgcctc aaatgacttc atttattatg ggactttgtg
     3061 cttctatgga agaacaaaat gctcaagcag aagaaggaaa ctttaatact gaatctatct
     3121 ccatgattaa aaccagttta atgggtcctt ttgctggaat tggtgattca tttttccaag
     3181 gtactttaag aattattaca tttgggattg gattgtcttt tgctaagcaa ggtagtattt
     3241 taggaccgat tttagctgtt ggcctttttg ctataccttc tattttagct gttttttacg
     3301 gcggtaaatt aggatatgag tctggcaata aataccttac taaattgtat caagaaggtc
     3361 ttatggatcg agtaatgcaa tttgcttcta tcattggttt ggctgtagta ggtggaatgg
     3421 tggctagcat ggtgccaatt gtcacccctt taaaattcac tacaggggga acagaacttg
     3481 ttttacaaga cttacttgat tccattattc ctggattatt accacttgta tgtactttgg
     3541 gaatatataa attgattaag aaaaatgtta gtacaaatat gctcttatta ggttgtattt
     3601 tggcagggtt tgttttaagt ataataggta tcttataatg attaaggctc taatttttga
     3661 tatggacgga gttttaattg atagcgagat tatgtatcaa aagtgggtat ttaatttttt
     3721 aaaacaagaa aatttaagct atcctttgta ttcttatcaa aaaaagattg gaactacagc
     3781 aagtattttt gatgatatag aaaagtacaa tgagggatgt aacagtttag aattgaaggg
     3841 aaaatttact aaatattgga aatcgcaaaa gattgattac ttggatattt ttcctaatat
     3901 actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata aaaatggcaa ttgcttcttc
     3961 cagtccaatg gatactataa atgaagttgt taccacttgt aaaatagagt catattttga
     4021 ttgtttaatt agcggaagag atttgccaga aagtaaacct aatcctacta tattccttaa
     4081 agcttcagaa cagctcagaa ttccagttga agaatgtctg gtgatagaag attcttataa
     4141 tggtataaaa gctggtaaaa gagcaaatat gaaggttctt gcaattaaag ataaacgttt
     4201 ttctcaagat gtaagtctag cagatgatgt tatttacgat attaaatatc tgcgacaaga
     4261 agtacaggtg aaatttggaa tataaataag ttttttgatg tatttagaga atattagtaa
     4321 aaataaaaat catgcccaaa tctaatcaaa attgattgct ttgggtatga tttcttttaa
     4381 tatcaatcta ttactttagg atctgaatca tttttatgca ccgtatctaa agtgatattt
     4441 tctgtgactt caataacata actaattcta tcacaacgaa ctgtggattg agaataatga
     4501 aatggttgat tatttttatc tttgccaacc ttttctaaaa taaatagcgg atctcctact
     4561 atacattgca ataagtcgga aagctcaggt gttgcagata aaccgtttat ttccataata
     4621 gagctcgtag ctttgacatc atagtcatct tctagaagtt tatataatga agtatattgg
     4681 ctaccttttt caaataaagt tggaaatttt tcttctgatg cgaaaaaatt atcaattccc
     4741 atcggggcac cgtcttccca cattaaacgt ttaatattgt aaactctatc agttacattg
     4801 atttgtaatt tattagctaa aaactcatca gcctttataa ttttagaatg taaaacagta
     4861 tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa atccatgaac tccagctaat
     4921 tttaaacttt cttttttttg agttacaaag gtccctttcc cccttaatcg ttcaactgtt
     4981 ccactttctg ctaaaagttt aaaagctttt cgaatggtaa tcttacttac tccataaatt
     5041 ttacataatt cttcttctgt aggtaattgc tctcctggaa gatatttttt ttcacgaata
     5101 tcgttacgta tagaagcttc tacttgtaaa tataatggtc tattatcttg aaaattcata
     5161 gtacactccc taaaaagtta tacttcatta tatcacaatt tattaaaacg tcgattgtaa
     5221 aattaagcta gacaactaaa aagatcattt gtgctacact caaaatagtt ccgaccaaag
     5281 aattaataag gagtgagtac aaatgactta cacccatctt acaacagacg aactagtaat
     5341 catagagtct tatttcaata tcgagaaacc tgtcgctctt gtcgcacaaa gtttaaatcg
     5401 ctctagacaa accatttata atgtttattc gtttctcaaa caaggaaaaa aagtgctaga
     5461 ttattaccgt caatacaaac aaaataaatc tcgttgtgga cgacgttcaa ccgttttatc
     5521 tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa ggatggtcac ccgatgtgat
     5581 tatcggccgt ggcgagtttc caattgattg ttctgttcgt acactctatc ggatgtttaa
     5641 aaaaggcctt tttgacacac agtcgttacc tatgaagggg aaacgtaaac cgaataatca
     5701 tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt catgaacgga acagtctcta
     5761 tccacacctg actaaagaat ttggtcatct cgaag
//
LOCUS       DACCVU010000153         5769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCVU010000153 DACCVU010000000
VERSION     DACCVU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.155"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            105..1001
                     /locus_tag="IVW29_002691"
     CDS             105..1001
                     /locus_tag="IVW29_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7840135.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1151..2092)
                     /locus_tag="IVW29_002692"
     CDS             complement(1151..2092)
                     /locus_tag="IVW29_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7840136.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2218..3339
                     /locus_tag="IVW29_002693"
     CDS             2218..3339
                     /locus_tag="IVW29_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7840137.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3597..5099
                     /locus_tag="IVW29_002694"
     CDS             3597..5099
                     /locus_tag="IVW29_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7840138.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5166..5693
                     /locus_tag="IVW29_002695"
     CDS             5166..5693
                     /locus_tag="IVW29_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7840139.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1886 a         1023 c         1219 g         1641 t
ORIGIN      
        1 caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac
       61 aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg
      121 tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg
      181 tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg
      241 caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg
      301 tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac
      361 acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg
      421 attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata
      481 tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga
      541 gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact
      601 atttcattct tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc
      661 tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag
      721 tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag
      781 cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt
      841 tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg
      901 aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg
      961 agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga
     1021 gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt
     1081 tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc
     1141 agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa
     1201 aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga
     1261 tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat
     1321 gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat
     1381 ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca
     1441 tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata
     1501 aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc
     1561 tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt
     1621 cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc
     1681 cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca
     1741 catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat
     1801 tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta
     1861 atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg
     1921 gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg
     1981 cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca
     2041 tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc
     2101 tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt
     2161 tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg
     2221 gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag
     2281 gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
     2341 ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt
     2401 gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa
     2461 ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa
     2521 ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat
     2581 gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat
     2641 ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat
     2701 cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta
     2761 aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg
     2821 caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca
     2881 ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga
     2941 agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct
     3001 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
     3061 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
     3121 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     3181 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
     3241 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     3301 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     3361 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     3421 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     3481 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     3541 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     3601 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     3661 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     3721 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     3781 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     3841 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     3901 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     3961 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     4021 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     4081 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     4141 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     4201 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     4261 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     4321 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     4381 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     4441 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     4501 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     4561 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     4621 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     4681 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     4741 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     4801 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     4861 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     4921 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     4981 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     5041 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     5101 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     5161 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     5221 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     5281 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     5341 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     5401 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     5461 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     5521 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     5581 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     5641 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     5701 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta
     5761 ggttatgac
//
LOCUS       DACCVU010000154         5689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCVU010000154 DACCVU010000000
VERSION     DACCVU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.156"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(488..1297)
                     /locus_tag="IVW29_002696"
     CDS             complement(488..1297)
                     /locus_tag="IVW29_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7840140.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="IVW29_002697"
     CDS             complement(1297..2043)
                     /locus_tag="IVW29_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7840141.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="IVW29_002698"
     CDS             complement(2061..2528)
                     /locus_tag="IVW29_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7840142.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="IVW29_002699"
     CDS             complement(2528..2938)
                     /locus_tag="IVW29_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7840143.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="IVW29_002700"
     CDS             complement(2949..3962)
                     /locus_tag="IVW29_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7840144.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="IVW29_002701"
     CDS             4179..4913
                     /locus_tag="IVW29_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840145.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="IVW29_002702"
     CDS             4897..5607
                     /locus_tag="IVW29_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840146.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DACCVU010000155         5644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCVU010000155 DACCVU010000000
VERSION     DACCVU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.157"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(57..350)
                     /locus_tag="IVW29_002703"
     CDS             complement(57..350)
                     /locus_tag="IVW29_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7840147.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IVW29_002704"
     CDS             complement(378..1043)
                     /locus_tag="IVW29_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7840148.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IVW29_002705"
     CDS             complement(1183..1815)
                     /locus_tag="IVW29_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7840149.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IVW29_002706"
     CDS             complement(1822..2889)
                     /locus_tag="IVW29_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7840150.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IVW29_002707"
     CDS             complement(2886..3617)
                     /locus_tag="IVW29_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7840151.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVW29_002708"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVW29_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7840152.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVW29_002709"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVW29_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7840153.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1605 a         1197 c          861 g         1981 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaat
//
LOCUS       DACCVU010000156         5626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCVU010000156 DACCVU010000000
VERSION     DACCVU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.158"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..352)
                     /locus_tag="IVW29_002710"
     CDS             complement(<1..352)
                     /locus_tag="IVW29_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840154.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDY"
     gene            554..853
                     /locus_tag="IVW29_002711"
     CDS             554..853
                     /locus_tag="IVW29_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840155.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1118..1993
                     /locus_tag="IVW29_002712"
     CDS             1118..1993
                     /locus_tag="IVW29_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840156.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1990..2208
                     /locus_tag="IVW29_002713"
     CDS             1990..2208
                     /locus_tag="IVW29_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840157.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2195..4822
                     /locus_tag="IVW29_002714"
     CDS             2195..4822
                     /locus_tag="IVW29_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840158.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4837..5055
                     /locus_tag="IVW29_002715"
     CDS             4837..5055
                     /locus_tag="IVW29_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840159.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2170 a          708 c         1088 g         1660 t
ORIGIN      
        1 catagtcttt atctagcgaa aacgtatcac tatatctttc ttgaatgtct ttaaagactt
       61 tgtgaggtat ttctgggaag tccttaatat atagctcata ccctatatcg ataaggtatg
      121 aagttatata ttcttctaaa gttccccatt taatccagtc aattgttgaa ttagtgatag
      181 ttgataataa tttcagcttt tgtggatttg gtaaattggt cccacgttcc cagttggata
      241 tggtcgcacg tggtgaatta tctattttcc ttccaagttc ttccatagta aaaccacatg
      301 aaaggcgtaa ttgtttgatt cgttttcctg cttctgaagg atttggcttc atcttttttc
      361 tcctttttat ttttaaagat agtatatcat atttataaaa taaaagtatt taaaagtatt
      421 tactttttat ctggagtatg atactttatt cttataaata caaataaata cattttttta
      481 cgcaatttgt atttatttgt acttataaag gtagaggagt tatagaataa ttaattaaaa
      541 aggagaggtt tttgtggaag caataaaatt aatgatttca gaagaacagg aggaaatgtt
      601 tctaaagcag ttttatcaaa ttgctacaaa agctattgaa aaggctcaag aaaatgcggg
      661 gatgacaagg gaatatctca atcaaaaaga aatggcggaa tacttaaatg tgagtgaaaa
      721 ctttttaaaa aatgctgtga gaaatgaggg attgcccgta gttgaattag gggcaagaaa
      781 attttattcg aaaaaggcag ttaatgagtt tatgtttgct cgaatgaaaa gaggggaaaa
      841 tattggaaga tgatgaagta gaaattataa aaaaatgctt taaaaaaaga aaaaaagtgt
      901 tcccaaaagt ttgcagactg ggggaacact caataataaa gtgctgagaa aactttattt
      961 atagctatta tatcaaaaaa gaaggtgaaa aggtgttttc ttctggaaat gttgtagttg
     1021 acaaacttag agaaataaat tttgacggaa atgtaatcca cacacatggt acgatcaatt
     1081 aagaaaaaaa acgaaaaaag gtgttgagaa gccctatttg aatgcaatag ttatcttagc
     1141 agaaattacc tattggtata gacctaaaaa aatttttaac gatgatggtc agctacttgg
     1201 ttatcaaaaa aagtttcttg aagatatttt gcaaaaatca tataagcaat taagcaaaaa
     1261 aacaggatta tcagaacgag ctataaaaga tgcaattgtg tacttagaga aaaaggggat
     1321 tatcaaaagg gtttttagaa atatttatta tgagaaaaga gcagttacat tatcaaatgt
     1381 tatgtacatt gttttaaatg tacataaatt gatagaaatt acttttccta ctttggattg
     1441 cgaaaaaaag gagtatatgc cgaaaattcg tcagaggggt atcgaaaaaa agagatatgt
     1501 ctctttaaaa aaaggtagag gtatgtacaa atttgtatat acgtatacaa agactacttc
     1561 acagaacata tcaaagacta ctctcgttat ggaagaagag gatattaata taaagaggat
     1621 ttacagtaag attagtgttc tttatgacgc aaataacgaa gatggtcgaa aattatttca
     1681 agtgtatcac tatttgagaa acgctaatgt agatacagaa gatatcagaa aaatcattga
     1741 atatttatct gaaaatactc agtatcttat tgctgagtgt attataaaac aacatgaagc
     1801 atgctttttt caaagaaaaa ctgaagaagg gttatacgat tatgtaacat attttttgaa
     1861 tggacttcag aaaagatata aaagtcaatt tttagaaaat gtagagccta tagatcagtt
     1921 caaaaaagct ttcgaaatca aagagttacc tgaaattact atgcataatt ggttgaaagg
     1981 agaagaatca tgattaaaaa tcgcttaata gatcaaatgt ggtgtcttga tcaaaataca
     2041 gatactctcg aattaatcga gagtatctgc ttcaacacag tagttctcga tttaagagct
     2101 gaaaatgaca attgtgtaac tcatatcata attaatcaaa aaatggctca gcagctctct
     2161 gagagcctaa gaaaatgggc tgaaggagaa aactatgaaa gcaattaaag aaaaaatcgt
     2221 tagtagaaaa ataaactggt tagcactcag tgtacttgtt ttaatgatta gcgttgtttt
     2281 tacaagtaat attgttgaag ctagcatttt caattggttt gatgacacaa aaagtcaaac
     2341 agagtttttg aataattctc tttatagacc ttacctaaaa aaatacaatg gagatttcct
     2401 tagtcagttc gggatttggc ttggatgggc tgtggtaaaa ggcatgttta ctgtgactga
     2461 ttcgattcaa aatatgattc cagatgtgtt ggatttgttc aatttcattg aaagtacagg
     2521 gctaaataat gtttatcaat cagtcataaa tacaattgtc gttggattaa tgatcctttc
     2581 attgatgttt gttggctaca agatgattac tggaaaagga acaatagatt taagatctgt
     2641 tggaatgaat atagtgatgt ctgtagcgtt aattctttta atgccaacaa tgatttctag
     2701 cggtattcaa tttgccaaaa tattttacaa tgatgctact acgattacaa attcagatga
     2761 cggagtcgct tggtcattga taaaacaagg agtaacagat ctggcatata ttaataaaac
     2821 agatcaatac tcaagcattg ataaaacgga ggatcgaaat aagttaacac gaaaaaattt
     2881 tcaacagaca gatttaacac aagtgttgac ggataaagtg attgataaat tggaaaaaga
     2941 aaatccagct gcggataatc ttcgctatga attagttgaa aattctaata atgaatttgt
     3001 tgccacaaag ttttctgata acttcttatc aacattttct gatagcttga aatcaggtta
     3061 ttatcgatat caagcaaatc tttgggggat ttcgattggg ttgacagcgt tggcaatcgc
     3121 ctatgtgttc agcgcgtttg tgatcattac agcaatatta gaacttgcat ttaaacgtgt
     3181 gttaggtgtt cttgtttttg cgacagatat tgaaacagga caacgttcaa aagttgtttt
     3241 atccgatatt ttacaatgtt acttaacagt tggatttcaa gggtttggac tatctatgtt
     3301 tgctatgttt atcaattttt taaatgctgg acaaggtatc tccacaaata tttttataaa
     3361 aacaattgct tatatctgtg ctgtgtttgt gctgataaaa ggcagtggaa cagtcatgag
     3421 atactttggt gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg
     3481 tatgggcgca atgctgttta gaaaaggatc aactggattt aatcgtgcta aaggaagcgg
     3541 aaatggaaat ggatcagaca gtggagaagg agaagatcga aaaccagaaa agaatttcgg
     3601 cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg cccatgaacg
     3661 tggattatca ggtttagctt cagatggagc tacaatggct agtgaaagag caataaaacc
     3721 atttaagagt atgcgtgata tggctaatga taccaagaat aagttcaaag aaggtttaga
     3781 tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct atgttagcta aaaataaaga
     3841 agatgaaact ggaaaatatg ctgattccat gccaacacgt ctttcggata gaaaagatgg
     3901 tgctaaggtt gaaaatgccg atagaattat gtctagttct gaacgtatgc gtgaagcaat
     3961 gaaaaataat gctgaatcga ataataatcc tgtaagtgca attcagcaaa aagttcaaca
     4021 agatattgaa gaacgtaaaa atgctatgca cggtcaagct aagtctgctg aagaattaat
     4081 taatcagaag cgacaagaag ctaaatacac gcctgaagca atgaatcgtg aggaaatgtt
     4141 aaggaaacgt gtagaagggc gtacaggagc taatacggac gaaaaagaag cgttgaccaa
     4201 agaaagaatc caagaagcta aaaactcaaa taccggatta gaaaaattgg tcaaagaaaa
     4261 tctacaaaat tctacaagta gtcaaggtgg ccgatcagta gatgtccgag aaaatattca
     4321 aggttcattg aatggtcatg tcggacggaa tgttgatgta agagagaatt tacaaagctc
     4381 ttcaaatggt caaggtacta agacaattga tgttcgagaa aatgtacaaa attctacaag
     4441 tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaaattcta caagtagtca
     4501 agggtttaaa tcaatggatg taagagagaa tgtacaaagt tctacaagta gtcaaggatt
     4561 caaaacagta gatgttcggg agaacgttca aaaagatagt ggagaaatga aagaaaaaac
     4621 acaaaaaatc aatatcgttg aagacagaaa gtcagcgaaa aaatttgatt caaatcatga
     4681 aacagtgatt attgatagtg aaatccgtga aaatgataag ggatcaaaac caagacgaag
     4741 atttacctat gaagacaacg agttattcag agatacactt aatgatccta atccgctttt
     4801 cgataatcta ttaaaaaaat aaaaggggga attgtcatga aattaattct aaagattagt
     4861 acgcatgatt tcttagaact tgacgaatta tgtgaatcag taaagaacgc aacaaatgaa
     4921 aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa gatgaaacat
     4981 gagataaaaa acaacttgaa atattgttat ctagaaaaaa gtgaagagat aatccatgaa
     5041 ttaaatggct tttaaaaatt gtatttgaca tgaatttacg acaaggaatt agtggggatt
     5101 aagcgattat tctcttccac tttagagaaa taatgggtta aaaagccaga atacgtagag
     5161 caatattgac aacctataaa ggagctattg ttgaaaattg aagtaacaaa aagataaaga
     5221 gaggtttaca tctatcagat taaaagtgat agtacaccca tgaaacctgc aaaagagttg
     5281 aacctaatgt atatcggttt aactcttttt atttgcaaat taaaaaaata tcaccgaagt
     5341 gacgaatgaa actatgacca aattaagcaa gaaaatatat cttgcaaata aagtagtata
     5401 aaaagatatt gataaagcca atcaaaactt tgaatttcaa acgttagata atcaaacaga
     5461 tacaaaaaat gaaatcagtt ctacgcgtac gattattaaa aataataaat tttcactaga
     5521 agaaaatgga aataaaatca aataattagg aagaaaaaac aaagcaatta gaaaagcctg
     5581 caagcaaatt tgagtaatcg agatccaatt tttttaagga agttta
//
LOCUS       DACCVU010000157         5479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCVU010000157 DACCVU010000000
VERSION     DACCVU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.159"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IVW29_002716"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IVW29_002716"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IVW29_002717"
     CDS             complement(580..1353)
                     /locus_tag="IVW29_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7840160.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IVW29_002718"
     CDS             1497..1847
                     /locus_tag="IVW29_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7840161.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IVW29_002719"
     CDS             1828..2544
                     /locus_tag="IVW29_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7840162.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IVW29_002720"
     CDS             2544..3992
                     /locus_tag="IVW29_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7840163.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IVW29_002721"
     CDS             complement(4062..4571)
                     /locus_tag="IVW29_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840164.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IVW29_002722"
     CDS             complement(4561..5286)
                     /locus_tag="IVW29_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840165.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1863 a          955 c         1096 g         1565 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacagataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataatt
//
LOCUS       DACCVU010000158         5433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCVU010000158 DACCVU010000000
VERSION     DACCVU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.160"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..177
                     /locus_tag="IVW29_002723"
     CDS             <1..177
                     /locus_tag="IVW29_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840166.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IVW29_002724"
     CDS             190..690
                     /locus_tag="IVW29_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7840167.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IVW29_002725"
     CDS             787..2634
                     /locus_tag="IVW29_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP7840168.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IVW29_002726"
     CDS             2637..3533
                     /locus_tag="IVW29_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7840169.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IVW29_002727"
     CDS             3583..3972
                     /locus_tag="IVW29_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7840170.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5433
                     /locus_tag="IVW29_002728"
     CDS             3959..>5433
                     /locus_tag="IVW29_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP7840171.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1856 a          784 c         1152 g         1641 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DACCVU010000159         5387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCVU010000159 DACCVU010000000
VERSION     DACCVU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.161"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(749..1501)
                     /gene="xth"
                     /locus_tag="IVW29_002729"
     CDS             complement(749..1501)
                     /gene="xth"
                     /locus_tag="IVW29_002729"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7840172.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1614..2534
                     /locus_tag="IVW29_002730"
     CDS             1614..2534
                     /locus_tag="IVW29_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7840173.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2607..3542
                     /gene="murB"
                     /locus_tag="IVW29_002731"
     CDS             2607..3542
                     /gene="murB"
                     /locus_tag="IVW29_002731"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7840174.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3840..5318
                     /locus_tag="IVW29_002732"
     CDS             3840..5318
                     /locus_tag="IVW29_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7840175.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1866 a          881 c         1032 g         1608 t
ORIGIN      
        1 actcattcct tgtgatttgg agttagatgg ggatcatgac ttctcggtat taagtcaaat
       61 ttatgcaaag ttgtgaggaa ccattttcaa aaaaatttct tacgactcga aagtaggcga
      121 ctttttcaca acttctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
      181 aaaatagcaa aatatgagga actattttgc catttgtttc tctaatactc aggtcaggac
      241 gctgatttca caacttctca ataaacggtg ttcaaaagct tgcatctgcg gtaataagtc
      301 caaccaaacg aaaaatatga ggaactattt ttgtttggct gttcttatta cttgatgcag
      361 tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa gcttgcatct gcggtaataa
      421 gcccaaccaa acgaaaaata tgaggaacta tttttgtttg gctgttctta ttacttgatg
      481 cagtgcagct ttttcacaac ctcttaataa acggtgttca aaagcttgta tctgcggtaa
      541 taagcccaac caaacgaaaa atatgaggaa ctatttttgt ttggctgttc ttattacttg
      601 atgcaggaca gctttttcac aatctctcga taaacagtgt tcaaaagcca gtttctcggt
      661 attatgccaa atttatgcaa aatgtggaaa gccattttca caaaattttt cttaatactc
      721 gaaactaaac gactttttca caacctcttt atttcaaatc taattctact ggacaatgat
      781 cactgcccat aatgtccgta tggatctttg catcagctag tcctggctct aaatctttgc
      841 tcgtcaaaaa gtaatcgatc cgccaaccag cattgttttt ccttgaattg aatcggtagt
      901 tccaccaaga gtagacacct tcctgggttg gataaaaata acggaatgta tcgataaacc
      961 cgttatccaa cagtcgagaa agagccgctc tttcttctgg agtgaatcca gcattttttt
     1021 gattcgtttt ccagtttttc aaatcaatct tttgatgagc gacatttaga tccccacaca
     1081 cgatgactgg tttttgtttt tttagatttt ccaaataatt ataaaaggct tcttcccatt
     1141 ctaatcggta atctaaacgt tttaattcgc tttgagaatt tggcgtgtaa caggttacaa
     1201 gaaagaaatc ggaatattcc aaagtaatca gacggccttc tgtatcatgg atatcgatac
     1261 ccattccgta agtggcattc aatgctggtt ttttagcaaa aatcgctgtg ccggaatagc
     1321 cttttcgttc cgcgtaattc caatattgat aataacctgg aagatctaaa tcaatttgtc
     1381 ctgcttggag tttcgtttcc tgcagacaga aaaaatcggc gtctaactca tgaaaaactt
     1441 ctaaaaaatt tttatttacg atggcacgaa gcccgtttac gttccaagag ataaatttca
     1501 ataaaagcac actccttcga attaatttcc ttacctttgc gatactattg tacctcattt
     1561 tagtaaaaaa tggtgtaaga tgaaagtaac taaatgatcc aggaggaaag tagatgaaga
     1621 ttggtgtaat cggtgtaggg aatatcgcag aaaaagcata tttaccaact tatgcgaaaa
     1681 aacaaggaac acttgatttt tattttgcaa caagaaataa agaaacaaaa gaaagaattc
     1741 aaaaagagta cgggttccaa catctatatg aaacgatcga tgagctttta gaagaaaaaa
     1801 tcgaagcatg tatgatccac gcagcaacaa aagtacatta tgaattagcc aaaaaatgtc
     1861 tagaaaatgg tatccatgtt tatattgaca aaccactgag tgtccatcta aatgaagttc
     1921 gtgaattgca agaattagct gataagaacc aaataatttt gatgatcggt ttcaaccggc
     1981 gctttgctcc tatggtagaa gaattgaaac gtatcccaga taagcggttg atccacctgc
     2041 aaaaaaatcg aatagcggca aaagaaacga cagagttcgt cctttatgat ttgtttttac
     2101 atcttattga tacagctgtg tatttattag atgaacccat tttgagaaca aaataccaga
     2161 taagagaaac aaaggaattt ttggaatatg ccattttaca attagaaaca caaaatcaaa
     2221 cggctatttt aacgatggat ttagcaagtg gcgctaatat agaaaaatat caagtaacaa
     2281 gtaaaaatgg tacttatgaa gtaaacgatc tgacccagct tgtcattcaa gatgctgatg
     2341 gaaaaaaagt gaaagaattt ggcgactgga caaacacgct agtcaaacgc gggtttgagc
     2401 caatggtaga ggccttcttt tctcagatcc gtttaggtaa atcggaaaca gaattgctta
     2461 aacaaaaaga agttgtaaga agtcatgaat tatgtgagga agtgcttaaa gatcactacc
     2521 gtcatcaact ttgaacaaga ctggcaagat tgtattaagc gtgatagaat aatgaggacg
     2581 tttgagaaaa gaaaggaaaa tcctcagtga ataaagaaga aatagttgaa agcttagcca
     2641 cgattactat attgtttgat gaaccattga tgaactatac gtttacgaaa acaggtggac
     2701 cagctgatgt ccttgcattc ccgaaaaaac aagaagaagt aaagcaaatc atcgattatt
     2761 gccgaatcca tgatatacct tggatggtat taggaaatgc aagcaatctg atcgttcaag
     2821 atggcgggat tcgcggtgta gtcatcatgc tgactgaaat gaaacagatt catgtcaaag
     2881 gaacgatggt cattgcggaa gcaggtgcct cactgattga tacgacttat gcagctttgg
     2941 cagaatcatt gactggattt gaatttgctt gcggcatccc gggaagtgtc ggtggagcgg
     3001 tttatatgaa tgcaggtgcg tatggcggag agatcaaaga tgtttttgct gaagtggatc
     3061 ttttgcttga agatggaaca ttgaaaactt tgacaaaaga cgaaatggta ttttcttatc
     3121 gtcacagtaa agtccaagaa ttacgtgcta tcgtattgga agctcgattc agtttgcaaa
     3181 caggggatta tgaagcaatc aaagcaagaa tggatgagtt gaccgaattg cgtcaatcaa
     3241 aacagccatt agaataccct tcatgcggaa gtgtctttaa gcgcccagta ggacactata
     3301 caggtcagct gatccaacaa gcaggattgc aaggcctgaa atggggcggt gcacaagtct
     3361 ctgaaaaaca tgctggattc atcgtcaata tcgatcatgc gacagcgaca gattatattg
     3421 aattgattgc gcacatccaa gaagtaatca aagaaaaatt tgatgtgtct ttagaaacag
     3481 aagtgcggat cattggagaa aaacaagcaa tatcagaaac aagcagaagt ttagccaatt
     3541 aataagtaat ggaacttaaa ataagtaaaa gagcaggacg gtggccgtcc tgctcttttt
     3601 tgtggttgtt tacgcaaatg gcgcaaatct tgtacatttt aacacaataa aacaaaaaat
     3661 atagaatatt tcctcataaa tttttccatt ttttgcaata ttctcaaaaa aaagataaaa
     3721 aatgagaata actgatgaaa aaacaagcat aatccatcat aaaatcagta taggtgaaaa
     3781 gatgaacttt catctgcata tcaagtaatt ggtatgaggc attttttgag gtgtaggata
     3841 tgaagttgga ttaccttata gaagaggaag atagagaaaa actgctgcta gctagatatt
     3901 tagaatttga gaaaaagaat tatgtagtgc tgcctaagtt atatgaaaca ttaggtttgt
     3961 ctaaatttaa aacaagaaat tatatcatag aattaaataa agaatttact gaattcaaaa
     4021 caaagccaga gatccaaatt tcagaagaag cagagatcca tgtcgaacgt ctgaatttga
     4081 cggatatcaa aaatttaaga agaatctact ttcaacgatc aaaaattgct cgactgttaa
     4141 cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat tttttaagca
     4201 tatcaagagc ctatgtaaaa agaagagaat tagtctcttt tcttgaacag catcaaataa
     4261 agttaaagaa aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg
     4321 tatatttcgg ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag
     4381 caaagagtat cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac
     4441 gaatcaaatt caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa
     4501 tcggaaaacg tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca
     4561 aggatttatc acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt
     4621 cttatatcat gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac
     4681 ttgcggaagc tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga
     4741 ttgatcaatt accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag
     4801 aacttcgtat gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga
     4861 ctagctttac gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta
     4921 cagcaatcca aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt
     4981 tcatgattcg cttattttat gattatctct ttttattggt cggtatttgt ccggtatcat
     5041 attttgaagc accgattcat gtctgtattg atttttcgca aggatctgct tataccgatt
     5101 acattatttc tcaagtgaaa ggatttaaaa attacaatat tgtgattgag agtcgaatca
     5161 cttctcgtac gaatatttat ttatctgatt gtttacttga aaaatttaat aaagaacaga
     5221 taatttggaa aaatccaccg actccagatg actgggaata ttttggaaat accatcatca
     5281 gagaaaaaaa tagacattta actgaaaaaa tcagctagta tttatggatg gcagctttgt
     5341 ctcacatatt tattagctga tttatgtttt tttcaaagaa aaggtaa
//
LOCUS       DACCVU010000160         5311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCVU010000160 DACCVU010000000
VERSION     DACCVU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.162"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IVW29_002733"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IVW29_002733"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7840176.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="IVW29_002734"
     CDS             complement(1297..2133)
                     /locus_tag="IVW29_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840177.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="IVW29_002735"
     CDS             complement(2317..3534)
                     /locus_tag="IVW29_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7840178.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3917..4000
                     /locus_tag="IVW29_002736"
     tRNA            3917..4000
                     /locus_tag="IVW29_002736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5237)
                     /locus_tag="IVW29_002737"
                     /pseudo
     CDS             complement(4240..5237)
                     /locus_tag="IVW29_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DACCVU010000161         5176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCVU010000161 DACCVU010000000
VERSION     DACCVU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.163"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(136..1098)
                     /locus_tag="IVW29_002738"
     CDS             complement(136..1098)
                     /locus_tag="IVW29_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7840179.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="IVW29_002739"
     CDS             complement(1100..2539)
                     /locus_tag="IVW29_002739"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7840180.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="IVW29_002740"
     CDS             2724..4670
                     /locus_tag="IVW29_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7840181.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="IVW29_002741"
     CDS             4934..>5176
                     /locus_tag="IVW29_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7840182.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DACCVU010000162         5131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCVU010000162 DACCVU010000000
VERSION     DACCVU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.164"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(67..609)
                     /locus_tag="IVW29_002742"
     CDS             complement(67..609)
                     /locus_tag="IVW29_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7840183.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(614..841)
                     /locus_tag="IVW29_002743"
     CDS             complement(614..841)
                     /locus_tag="IVW29_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7840184.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(920..2800)
                     /locus_tag="IVW29_002744"
     CDS             complement(920..2800)
                     /locus_tag="IVW29_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7840185.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(2989..3603)
                     /locus_tag="IVW29_002745"
     CDS             complement(2989..3603)
                     /locus_tag="IVW29_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7840186.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            3745..4305
                     /locus_tag="IVW29_002746"
     CDS             3745..4305
                     /locus_tag="IVW29_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7840187.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(4610..4924)
                     /locus_tag="IVW29_002747"
                     /pseudo
     CDS             complement(4610..4924)
                     /locus_tag="IVW29_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         1539 a         1042 c          793 g         1757 t
ORIGIN      
        1 ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat
       61 agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag
      121 tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg
      181 caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg
      241 gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg
      301 accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc
      361 attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata
      421 ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg
      481 gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc
      541 agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac
      601 tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt
      661 ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt
      721 tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt
      781 cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca
      841 tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa
      901 aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata
      961 aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag
     1021 ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa
     1081 tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca
     1141 acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc
     1201 ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat
     1261 ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt
     1321 cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct acataatcag
     1381 cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact
     1441 tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga
     1501 tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat
     1561 cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg
     1621 cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat
     1681 ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc
     1741 gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt
     1801 catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag
     1861 ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga
     1921 ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa
     1981 tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac
     2041 taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc
     2101 gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa
     2161 ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg
     2221 ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac
     2281 cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa
     2341 tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt
     2401 cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag
     2461 ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa
     2521 acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag
     2581 caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga
     2641 tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga
     2701 caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga
     2761 tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt
     2821 cattcttacg agactaagta tagatactta tccatcataa aaaattgacc actatcaagt
     2881 ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag
     2941 actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat
     3001 tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat
     3061 tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc
     3121 tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt
     3181 accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg
     3241 cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt
     3301 actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc
     3361 aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc
     3421 gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata
     3481 tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc
     3541 aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt
     3601 catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt
     3661 gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga
     3721 tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa
     3781 aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact
     3841 atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac acattataag
     3901 gatatctatg atctatatga tcaattagaa agcaactttt ttcgggatct aaatgaaatt
     3961 ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat tgactatgtt
     4021 tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat ttcgtttcag
     4081 aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata tgaagacaag
     4141 aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca aggttgtcta
     4201 tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga attattagaa
     4261 ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa aacatactaa
     4321 gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt tagccaaatt
     4381 gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa aagtagtttg
     4441 tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa ttcacctcat
     4501 cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc tgtaaccaat
     4561 ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt tacctcaatt
     4621 cattttcaag gggaatcagc tcatcatcta acattttacg ccatgctcgc ttcatttctt
     4681 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
     4741 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
     4801 aagcatgtaa ttttttctca gaaacaaacc actcattgga aaagacatga atcaaatcat
     4861 cacttacttg tgtaaaatag cgacgtttaa tgacaaagat atcttcattg ctatctagtg
     4921 taccttcatt tctttgtgct taaattcaaa gataattttc tcaataaaga tttttgattc
     4981 cagtaagatt cctttactcc ttttacaaca tagcgttgtc ctttgttttg aaacatcttc
     5041 ttcccctctt tgccattaaa aatagcccaa ttctctataa agaagattaa gcttcattat
     5101 tataatataa agtttaaatt tggtttatta c
//
LOCUS       DACCVU010000163         5066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCVU010000163 DACCVU010000000
VERSION     DACCVU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.165"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..963)
                     /locus_tag="IVW29_002748"
     CDS             complement(16..963)
                     /locus_tag="IVW29_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7840188.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1944)
                     /locus_tag="IVW29_002749"
     CDS             complement(1141..1944)
                     /locus_tag="IVW29_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7840189.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2090..3004
                     /locus_tag="IVW29_002750"
     CDS             2090..3004
                     /locus_tag="IVW29_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7840190.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3052..3522
                     /locus_tag="IVW29_002751"
     CDS             3052..3522
                     /locus_tag="IVW29_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7840191.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3607..4215
                     /locus_tag="IVW29_002752"
     CDS             3607..4215
                     /locus_tag="IVW29_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7840192.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4349..4549
                     /locus_tag="IVW29_002753"
     CDS             4349..4549
                     /locus_tag="IVW29_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7840193.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1670 a          776 c          999 g         1621 t
ORIGIN      
        1 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
       61 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
      121 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
      181 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
      241 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
      301 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
      361 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
      421 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
      481 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
      541 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
      601 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
      661 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
      721 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
      781 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
      841 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
      901 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
      961 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     1021 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     1081 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     1141 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     1201 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     1261 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     1321 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     1381 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     1441 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     1501 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     1561 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     1621 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     1681 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     1741 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     1801 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     1861 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     1921 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     1981 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     2041 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     2101 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     2161 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     2221 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     2281 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     2341 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     2401 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     2461 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     2521 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     2581 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     2641 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     2701 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     2761 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
     2821 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
     2881 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
     2941 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
     3001 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
     3061 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
     3121 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
     3181 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
     3241 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
     3301 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
     3361 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
     3421 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
     3481 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
     3541 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
     3601 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
     3661 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
     3721 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
     3781 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
     3841 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
     3901 ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa
     3961 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
     4021 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
     4081 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
     4141 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
     4201 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
     4261 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
     4321 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
     4381 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
     4441 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
     4501 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
     4561 ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag tttttagatt
     4621 ttttggctaa gtaaatgcat ttgttcactt gacatgtcct aactaattat caagcaagga
     4681 acattttctt cgttagtaat ggattttaaa gaaatttcag tttttggatg ttgaataaaa
     4741 gaccatttgt gaaaccacta gtatacatac tcattaccat gtcaattcta ttactttaaa
     4801 agaaaatcgt tttactttga agaggagcat atctatccat taaaagatga tggtaatgca
     4861 atcactagtt cgagtcattt taagagccat gcagtgagct ggaaataaaa cgaatagaag
     4921 atgggtttaa caaaaggacc agtcatagga agaaattgtc gtgaaatcga atgataatct
     4981 gaacggtagg caaagactgt taaaaagatg ggaaaggtat gtgcgatcat tttactgcac
     5041 gattatttgg aaaatacctt gttgtt
//
LOCUS       DACCVU010000164         4931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCVU010000164 DACCVU010000000
VERSION     DACCVU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.166"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            151..531
                     /locus_tag="IVW29_002754"
     CDS             151..531
                     /locus_tag="IVW29_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7840194.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            513..1427
                     /locus_tag="IVW29_002755"
     CDS             513..1427
                     /locus_tag="IVW29_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7840195.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1667..2356
                     /locus_tag="IVW29_002756"
     CDS             1667..2356
                     /locus_tag="IVW29_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840196.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYKEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2477..2563)
                     /locus_tag="IVW29_002757"
     CDS             complement(2477..2563)
                     /locus_tag="IVW29_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7840197.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
     gene            3208..3945
                     /locus_tag="IVW29_002758"
     CDS             3208..3945
                     /locus_tag="IVW29_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7840198.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            3974..4495
                     /locus_tag="IVW29_002759"
     CDS             3974..4495
                     /locus_tag="IVW29_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7840199.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENN
                     NAITTEKKWWKFW"
     gene            4579..4883
                     /locus_tag="IVW29_002760"
                     /pseudo
     CDS             4579..4883
                     /locus_tag="IVW29_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1867 a          825 c          898 g         1341 t
ORIGIN      
        1 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
       61 ccttttggtg agctttactc attgtgcctt tttaggtaca atggtattgc gaacgatacg
      121 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
      181 gatcattcta aaaaatatac ctatcgatcc aagccaaaac aaatcagttt tcgagtgagc
      241 gaatctgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
      301 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
      361 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgccaag
      421 tggtgcaata cacaccaaca tgacgttgct cctggagacg cccaacgctt atccgaaaac
      481 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agccgaacca
      541 cttcttgtag tcgtgcaatt aattacgctg aaccacgagc aaccattaaa acaggaataa
      601 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
      661 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
      721 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaacag
      781 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
      841 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
      901 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaaac
      961 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     1021 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     1081 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     1141 cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     1201 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     1261 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     1321 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     1381 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     1441 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     1501 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     1561 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     1621 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     1681 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     1741 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     1801 ctgtatattt tccttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa
     1861 gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga
     1921 taaaaagaaa aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttataa
     1981 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     2041 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     2101 tgagcgaacc aaaaaagacg tgcaatatgt aaaagataca aagatagaag aagcctgtag
     2161 tgatctattt ggagacacaa acccttggtt tgccagtgaa gaagaaaaaa aagaaaaaca
     2221 acaatctgtc acatatgaac gctacgaaaa cctgtttcaa caagcaacta acatgcaaaa
     2281 tccgacaaaa aaagaaaccg ccttaaacta tctaaaaagt gtcaaggaat ggttagatca
     2341 gcaacagcaa aattgaattt gattgaaact catgttaaac tgaatgtgca aatgatcaaa
     2401 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat
     2461 gtcgccttta ctgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga
     2521 cacaaactcc tacaagaagg gggcaaatga actgttcaaa cattagatac acctccttcc
     2581 agtcgcaaga ctgtcggata ggcgacaagt tattataccc tacaaaatta aatctgtgtg
     2641 gcgattttaa gtatacctta ttttcgggtt gttagggctt gccctgaccg cctgtaaggc
     2701 gcttctcgac tagattaagg gagttaatca agaattgtct tagaacggct tagacaagct
     2761 ctcagattct cccacctcct ctttgaacaa cggttttagc aaaggagccg gaggcgactg
     2821 cgcaggtaca atgtgagagc gtagcgatca caccttttta gcgaccgtag ggagcaagtt
     2881 ttctagttta gaaaaattcg cgaacctttt tggaggtaaa agtaggagtt ttttaggcgg
     2941 gaaattaaat gtgctcttaa gatatactgt tagatatact gttagatata ctgttaataa
     3001 taactgttag ggattagcaa ccttatggga gagtaagtat tgacataggt agtgggggat
     3061 aaattgtcac actatggggg ataaattgtc acactatggg ggataaattg tcacgctatg
     3121 ggggataaat tgtcacacta tgggggataa attgtatttt acaccctctt atgtctatga
     3181 taacataatt tattgaggtg atttttattg agtaacgata tagttcgata taataatgga
     3241 ttaaattctg tccctttaag aaaatttacc cctatagata tggatttatt ttggggaata
     3301 tgttctaaaa tgaaaagaaa aggtacaaaa gaagaaattt ttacatttaa acaaataaaa
     3361 gaattaattg attacaatat aaatgcacgt ggcgaccgtt ttgctaatga tttaataaaa
     3421 atgtcaaaaa aaatcattac tttgtctttt atttatgaag acgaaaaaac atatgaacaa
     3481 ttaaatctgt ttcaaaaatt taaaatcgat aaagtgaatg aaacattatt aataaaggtt
     3541 tctgaagaat ttgaatatat acttaattca atcggaacta attttactcg tttcgaatta
     3601 gaaaatatga caaacttaaa gagtagctac gttaaagagt tatatcgcca acttatggct
     3661 cataaagaca gaagatctaa aaaaggtcac tggtttgtaa aaatagaaga ttttagagct
     3721 ctattagcaa taccagaaag ttatcgcatg agagatattg ataaacaaat ttttgcccaa
     3781 gcgaaaaaag aatttttaaa cgttaattca actcaccctg ctatttttaa aacttttgaa
     3841 atcaaaaaaa ttaaagccta caaaggaaat aagataggat ctctgcaaat ttattttgaa
     3901 gaaaatatac cttcaattac cttagataat tggttagaga aataataacc taacaattta
     3961 ggaggcaatt atcatgcaac ccaaagaaac ctttacaata aaggaattat ctgaattatt
     4021 tgaaataagt aggcaagcaa tttctaaaca tgttgataaa ttagacccgt ccttattagc
     4081 taaaaatgag cgtggttata aggttgtatt acgttctgga gtattgcaac ttgctagcaa
     4141 cttaggtaac caaaggttac tagataggtt gcatgagtat gaacaagaaa acgataaaaa
     4201 cgtttatata tcaagaaaaa acgtggttgt aaacgagttg ctagataagt tgcgtgaaga
     4261 aaatgaattt ttacgagaac aactttctct caaagagcaa aataattcta atatgcaaaa
     4321 attactcgac cagcaacaac aactcacctt gcaggcaaat caacagatcg aaaaactgca
     4381 agaacagtta caattagctt atacggaaga acctgaagaa gaggtattaa cttctgtcaa
     4441 taaagaaaac aacaatgcaa taacaacaga aaaaaagtgg tggaaatttt ggtaacaatt
     4501 accaactttc cataaagaag aaatcaattt ctatagactt tatctttcta aaaataaaaa
     4561 atatgtttag gtgatcaaat gactataagt aaagtaatca aacaaaaaag aatagaaaaa
     4621 caactaactc aagaagattt agctgaaatg cttttagtat ctaaaaaaac catttctaat
     4681 tgggaaaatg gtagaacaat accagataca gaaaatttaa ttcaatttgc taagttattt
     4741 gatttatcac tagacaatat tttgttagga gaagaaaaag ttatgttaaa aaatcataca
     4801 aaaacactaa tactattatt tcattttgta atttttttaa gtttagtttt taccttaatt
     4861 cagtccaaat tcttttattt tagccattca attgccactt ggttattaat attttttcta
     4921 agtttgctat t
//
LOCUS       DACCVU010000165         4642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCVU010000165 DACCVU010000000
VERSION     DACCVU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.167"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1014..1304)
                     /locus_tag="IVW29_002761"
     CDS             complement(1014..1304)
                     /locus_tag="IVW29_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840200.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="IVW29_002762"
     CDS             1646..1942
                     /locus_tag="IVW29_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840201.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="IVW29_002763"
     CDS             1949..3901
                     /locus_tag="IVW29_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7840202.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IVW29_002764"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IVW29_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7840203.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1589 a          815 c          757 g         1481 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt tt
//
LOCUS       DACCVU010000166         4619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCVU010000166 DACCVU010000000
VERSION     DACCVU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.168"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(379..636)
                     /locus_tag="IVW29_002765"
     CDS             complement(379..636)
                     /locus_tag="IVW29_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7840204.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(629..898)
                     /locus_tag="IVW29_002766"
     CDS             complement(629..898)
                     /locus_tag="IVW29_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7840205.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1029..1208)
                     /locus_tag="IVW29_002767"
     CDS             complement(1029..1208)
                     /locus_tag="IVW29_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840206.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1589..2296)
                     /locus_tag="IVW29_002768"
     CDS             complement(1589..2296)
                     /locus_tag="IVW29_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840207.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(2289..2726)
                     /locus_tag="IVW29_002769"
     CDS             complement(2289..2726)
                     /locus_tag="IVW29_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840208.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(2741..2992)
                     /locus_tag="IVW29_002770"
     CDS             complement(2741..2992)
                     /locus_tag="IVW29_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840209.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2992..3198)
                     /locus_tag="IVW29_002771"
     CDS             complement(2992..3198)
                     /locus_tag="IVW29_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7840210.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(3635..3895)
                     /locus_tag="IVW29_002772"
     CDS             complement(3635..3895)
                     /locus_tag="IVW29_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840211.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3970..4173
                     /locus_tag="IVW29_002773"
     CDS             3970..4173
                     /locus_tag="IVW29_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7840212.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(4314..>4619)
                     /gene="ssb"
                     /locus_tag="IVW29_002774"
     CDS             complement(4314..>4619)
                     /gene="ssb"
                     /locus_tag="IVW29_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7840213.1"
                     /translation="MATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQLLE
                     SKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLPF"
BASE COUNT         1293 a          916 c          743 g         1667 t
ORIGIN      
        1 tctatgagaa gtttattttt gttgtttttg ctaattaact aaaactagta caaaaaattt
       61 aaaatagatt attaaaccta ttgcttattc tattcagtca tatatataag cgtcatcctt
      121 tagtaactaa catcccacac tacaatagta ttataacata tctattcaac ttcaaattgt
      181 tgaaatttaa gccatttata tttacttttc ctcaacttta gaaatgattt tactaacctt
      241 atatccgtaa tacgcgtaac aaacacattc attaataaca atcaacacta agctatatta
      301 gctttttaaa tgactagggt aaataaaata aggatcaaaa acttttaagt ttctgaccct
      361 ttccttactt ccgattggtt aatagtgatc ttttgcagaa taaataaata tcgtttcatt
      421 ttcaactcta tatatcagtc tatgttcatc tgtaattctt ctggaccatt ttccagataa
      481 atcatgcttt aatggctcag gttttcctaa tccagcaaag ggggaacgat cgatatcttt
      541 tattaacttg ttaatctttt ttatattgct tttgtttcct tgctcatgcc aataaagata
      601 atcatcccaa gcatcatcag accaagcctt aatcatcaga ttcaacctcg attaagtcat
      661 gtgttttaaa tgcacctttg gagaattgtt catctcctcg acgaattttt tccatgacgt
      721 aattattaga aagtgttctc aacgtttctt gcatagaatc ataatctctt tttgataata
      781 caacaactgt atcttctaca tctttacttg ttacaataag tgtttcagca tcctcattaa
      841 cttgtttcat ataactacgt aaattttggc ggaaatttga ataagctact gcttccattc
      901 ctttcacccc ttatttattt ctataaaaca attgtacatt atattgtaca attaagcaaa
      961 tgattaatat tctaacttct tataattaaa agtgctatca cttttagttt ttaggataaa
     1021 aagtgacgtc actttttgag tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat
     1081 aaatctaacg caatacacat aatcctggat tcacttttgc cgtacttctg ctttagttgc
     1141 tgcaattctt tttgtctctt ttcatccaac gtcacgggta accgtttatt tcctattgca
     1201 attggcatta gtactctctc ctttcatcga aataacccta ttaaaaatat ggctgcaatc
     1261 aacttgaata ttagactcac aagggttaac acaaagcgcc aaaagatcaa acaaaatacc
     1321 agtattccta agaatactac gaagtttaaa tgtagagctg caccatgacg cataagtatc
     1381 tcgccaacaa acagtacgaa caatagagga atgattttat tcatatggat ctccttcttt
     1441 ctcttaggtt atcgggcttt gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg
     1501 gatcgattgg tgcgaaataa gaaccattcc cttaatttca aggttttttg ttcttatttc
     1561 acacatcctc acttttcggt ataaatggtt attgaccttt tttcctttca aatcgttgat
     1621 atggcattcg tttacggtca agttcatttt ggatttcttc tgtatcgtcg taagtgtagg
     1681 tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta
     1741 catattccct caaaaatgct tcgggtacag tgagttcata atccattccc attaacacga
     1801 tagattccgt cccacacgtc aaaaatctag taaatacaag gtaataatcg tctaccgtcg
     1861 catctttgct gtttagctct tccatttctt cttccattaa ttccatgctc tcaaaaattg
     1921 aataatattc tgattctgag taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt
     1981 cgaccagctc actcctttct aggtaaacat tcatgatata tttttctgtt tctagtcgta
     2041 gaagctccgc tccttttttt aatgtgtcag gacgacgttt atacacgtct aaacgattat
     2101 ggctgactgt aaccttcgtt tgactcgttt cactttccat atacaactgt tcttccactc
     2161 tttgaataaa gtacttcatt tcttccatac aactaaagtc tttttgaatc cgtctgatta
     2221 cttcattcaa gtgttctttg tttgttaaaa attgcgaaac atcttctgtt gttagctgat
     2281 aaaccacctt attcattatc tttctcctcc gattcaatcg tataaaatgc accatttttt
     2341 tcgatatttt caaaagtatc tagccagtcg ctagccattg ctttagcttt cttaatctgt
     2401 gcgaattttg cataagtcag ttctaaggct tgatattcgg aaggtttgat agtgcttggt
     2461 ttgacaccta cttccaagca tagtaaggct tggaaaaacc attttccatt ttgttttttc
     2521 agttcttcca gtgttcgata gtacgtcgca atgagattca tttcgacacc tgattcaatc
     2581 atttcttcca ctgcgactga caatttttcc ttgtaatgtt ttttgggtac acgtgtttca
     2641 tggtatagga acgtcatatc ctccattttt tgtgccattt tatccaaagg taatttgaca
     2701 aattcgtcat agtaattttg agccatattt tttctccttt ttagttctct aatttggtta
     2761 accaatattc gatttcataa gtttccatgt tttcttgata atgcagttca tatcctgcaa
     2821 tcttcactag gtcttcaaat agcatagtcg gtgcatcctc tactaaaggg gtttctatcg
     2881 tccagtaccg cggaataagt aaagcttctg ctactttttc agaaatgact tctttgtcca
     2941 ccaaaatttg aacgacttct tttacagtca ataatttgtg ttgtgttttc atcaccgttt
     3001 ctttactttc tcatcaatca aaacataatc gccataagac aaggtatatt ttttcccgca
     3061 tgttggacat tgatacaaac gatagctttt gatacatttc gctctttctc cacaatcgca
     3121 ttcgacagta tgtggtgcat gatagtttgg gaataattcg ctgaagggaa caccccaata
     3181 atcaaatttc tttcgcatga ttgattctgc ctcctcttag gaaaggtttt atttcctttc
     3241 gttagttctt tctttattga tttgttggcc agaatgaatc aacgaaaagc gacaaggaca
     3301 agggcgcgca gcgtgccatt tacccttgtc cttgtcgctt tttggtgata aaaggcaact
     3361 tgttgccggt ctggcaaatc aataaagaga tagcgaacag gaacgaacct tttggtgaga
     3421 ggaagttttt tctattttgt cttgctttgc aagacaccat atcttagtga acgtgcaacg
     3481 ttcacttcct ttcttctcga acggcggtgg cggttggagt gagagaaagg tttttcaatt
     3541 gagaaggcac ttgtgtcttc ctaaaaaggt cttttcctgt ttctatgtaa aacaaaaaga
     3601 gctgcaatcc tcatttagga ctgcagctct tcttttatat gcccatcgat cgcgtaaaat
     3661 gatcggggtt tctaatttgc atcgattcaa tgactaattg attccgttta ttgaaatgac
     3721 ctgtgacggc aatattgtat tttccatcat ccataattaa caatttatcg ataatttcaa
     3781 tatctgcaac tacacaatta atcgattcat gaattgtttg taaagtaaag cgaaccaata
     3841 atggttgtgt ttttattact cggattttat caagcaaacc aacatatttg ttcatcacga
     3901 tcacctcgct aaaattatag caaaaacagc taaaattgag aatttttatt cgattttaga
     3961 tatgctataa tgatcaagac agtaaagttt gtgacggtgg ctatccgatt gaaagtgagg
     4021 tggtgcgtat gtgcgtactt ccaaaatctt acagaaagga gaagcctttt gcctgttgaa
     4081 acggcgctag gattaatgat tagtttcggt tctttgatcg caacaatcat ttttggaatc
     4141 ttagcagttg taaacaacaa tcaaaaaaaa taaccgtcag ctttagccgg tcgacgatta
     4201 tttttcaaaa ataatattaa gctggtcacc gtcttaaacg gtgctgttag gggagttgtt
     4261 agcgcaactc ctcttttcgt atccattata acaactttat tgatctttta caatcagaat
     4321 ggtaaatcat catcactaat atcgattgat tgtccgccaa atggattttg tgaagcgttc
     4381 aatccattgt tgttaggcgt agctacatcc cgttgttcaa aggtggtagg gcttggactg
     4441 ctggacgatt gatcagcgtg cgcacggctt tctgtcgctg ctttactttc taatagttgg
     4501 aaattatcgg ccacaacttc tgtcacatac acacgttgtc cttgttggtt atcgtaggtt
     4561 cgggtttgaa tccgtccaac aacgccgatc aaactgccct ttttagctaa tgtcgccat
//
LOCUS       DACCVU010000167         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCVU010000167 DACCVU010000000
VERSION     DACCVU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.169"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            33..1094
                     /locus_tag="IVW29_002775"
     CDS             33..1094
                     /locus_tag="IVW29_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7840214.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="IVW29_002776"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="IVW29_002776"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7840215.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="IVW29_002777"
     CDS             2007..3989
                     /locus_tag="IVW29_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7840216.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="IVW29_002778"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="IVW29_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7840217.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1493 a          820 c         1046 g         1252 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg t
//
LOCUS       DACCVU010000168         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCVU010000168 DACCVU010000000
VERSION     DACCVU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.170"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            574..1008
                     /locus_tag="IVW29_002779"
     CDS             574..1008
                     /locus_tag="IVW29_002779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840218.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IVW29_002780"
     CDS             2298..3221
                     /locus_tag="IVW29_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7840219.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IVW29_002781"
     CDS             3237..3776
                     /locus_tag="IVW29_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7840220.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACCVU010000169         4410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCVU010000169 DACCVU010000000
VERSION     DACCVU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.171"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            150..512
                     /locus_tag="IVW29_002782"
     CDS             150..512
                     /locus_tag="IVW29_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7840221.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            521..1273
                     /locus_tag="IVW29_002783"
     CDS             521..1273
                     /locus_tag="IVW29_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7840222.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            1287..3089
                     /locus_tag="IVW29_002784"
     CDS             1287..3089
                     /locus_tag="IVW29_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7840223.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            3366..4406
                     /locus_tag="IVW29_002785"
     CDS             3366..4406
                     /locus_tag="IVW29_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7840224.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         1444 a          759 c          933 g         1274 t
ORIGIN      
        1 agacctccag acttcttgat agctcaagta tatcttgagg tgcatttgtt agtaaggtac
       61 tgttctatgg ggcgcttacg ttgattcgaa ggcagaattg ttcgcttttt tctataatca
      121 gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt ctacttgttt
      181 tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg ggtggagaag
      241 aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca gactctggac
      301 aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt aaggaaaaag
      361 aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat ttaaaaatca
      421 cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa gacgtgccga
      481 aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa tagtcgaagt
      541 acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag cattaagcgg
      601 catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt ctggttctgg
      661 taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg ggcatatccg
      721 aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt ttcgtgcgaa
      781 tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag cacaagaaaa
      841 tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc aacgtgtcca
      901 aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt ctgagatttc
      961 cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac caacgattct
     1021 attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc ttttagatac
     1081 gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc atgatccgtt
     1141 ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc atcaagaagt
     1201 gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta ttcttggaaa
     1261 cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact gggatcaaga
     1321 gccgtttaag agactacttg gtgttattct caggattagt tatggcatct gctatttttt
     1381 acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg atcgttgcgg
     1441 ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca ttcgtttata
     1501 ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca atgtttatga
     1561 tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca ttcttagtag
     1621 ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt atcgtccacc
     1681 gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt agtattcacg
     1741 ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct gttgtcaatg
     1801 ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag acaccaacaa
     1861 gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt gcttttctag
     1921 gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt ataggggtag
     1981 ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc attttctttt
     2041 tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat ttcacattgt
     2101 cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc gctatgctgt
     2161 ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa attgcagact
     2221 tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca gaagatcagc
     2281 aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa gaagatgcgg
     2341 gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg ataaaacatg
     2401 aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa atgaatgaac
     2461 tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa aaagaaatca
     2521 aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact ttacagctag
     2581 ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt gctcaaaata
     2641 aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat gaaatgtatg
     2701 aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta ggtttagctt
     2761 tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc aagagtgaca
     2821 ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt aaacaatcga
     2881 tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta gtccatgtct
     2941 tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat atttgggttc
     3001 cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca tggttatata
     3061 ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca
     3121 gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc gcagatgcaa
     3181 gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt cacaaccttt
     3241 ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg gaataagctg
     3301 ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa gagggtgcaa
     3361 ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt acgatcacga
     3421 atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt cattttcaaa
     3481 caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca aaagaatatc
     3541 tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg attacgaatg
     3601 gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac actggtcatt
     3661 gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca gtcatcaatg
     3721 gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa aacggatttc
     3781 ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca tggcgagtga
     3841 cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat gtctatttca
     3901 atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg aatagccgat
     3961 tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca gtaagtggca
     4021 cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt gattttcgcc
     4081 aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa tctggtttac
     4141 taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta aaaacagatg
     4201 cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa atgcgcggga
     4261 ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca ggagcggagc
     4321 gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag tttgtaacgg
     4381 aatacaaaat aaatataaag aaatagaata
//
LOCUS       DACCVU010000170         4361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCVU010000170 DACCVU010000000
VERSION     DACCVU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.172"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            15..245
                     /locus_tag="IVW29_002786"
     CDS             15..245
                     /locus_tag="IVW29_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7840225.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            342..1643
                     /gene="murA"
                     /locus_tag="IVW29_002787"
     CDS             342..1643
                     /gene="murA"
                     /locus_tag="IVW29_002787"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7840226.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1656..1832
                     /locus_tag="IVW29_002788"
     CDS             1656..1832
                     /locus_tag="IVW29_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7840227.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1967..2263
                     /gene="yidD"
                     /locus_tag="IVW29_002789"
     CDS             1967..2263
                     /gene="yidD"
                     /locus_tag="IVW29_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7840228.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2327..2710
                     /locus_tag="IVW29_002790"
     CDS             2327..2710
                     /locus_tag="IVW29_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7840229.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            2879..3763
                     /locus_tag="IVW29_002791"
     CDS             2879..3763
                     /locus_tag="IVW29_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7840230.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3880..4113
                     /locus_tag="IVW29_002792"
     CDS             3880..4113
                     /locus_tag="IVW29_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7840231.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4201..4281
                     /locus_tag="IVW29_002793"
     tRNA            4201..4281
                     /locus_tag="IVW29_002793"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Tyr,seq:gta)
     gene            4288..4359
                     /locus_tag="IVW29_002794"
     tRNA            4288..4359
                     /locus_tag="IVW29_002794"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4320..4322,aa:Gln,seq:ttg)
BASE COUNT         1476 a          685 c          977 g         1223 t
ORIGIN      
        1 gaaaggacgt cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc gtgagtcatt
       61 tggcgtttat ctacttagcc ttctggtctt tacgttcgtt acgcattgaa acttttttca
      121 gaccaatgca taatacgcaa attagaatgg caatcgtact tttttcaatt tttttagggt
      181 acactgcaag taattttttc ttagaagtta tcgctttatg ccgaaatctt ttcgttagcc
      241 tgtaaatttt aaaaaaaggt actattttaa aagcaaggat gttataatag aaaagatttt
      301 gagtaagtgt atgctcattg gaattttttg gagggaacaa catggaagag atcatcgtaa
      361 gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg agctaaaaat gctgttttgc
      421 ctattttagc tgctagtttg ttagcagaag aaggtattac gacattagac aacgttccga
      481 ttctttctga tgtcttcact atgaatcaag tgattcgtca cttaaacgta gatgtagatt
      541 ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag acaattagaa atcgaagcgc
      601 cttatgagta tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg ctacttgcca
      661 gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa cgaccaatcg
      721 atttgcattt aaaaggattt caagctttag gagcaaaaat tattcaaaaa aacggctata
      781 tcgaagcaat tgcggatgaa ttgatcggta acacgattta tttagatttc cctagtgtgg
      841 gtgctactca aaatatcatg atggctgccg taaaagcaaa aggaacaacc atcattgaaa
      901 acgtagctcg cgaaccagaa atcgttgatt tagcaaacat tttgaataaa atgggcgctc
      961 aagtctatgg agctggtact gaaacaatgc gtattgaagg cgtggatcat ttacatgcag
     1021 tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt gctgctgcaa
     1081 tgacacaagg caatgtgctg attgcagatg ctatctctga acataactgc ccattgattt
     1141 ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc cgtgtcatcg
     1201 gaccaaaaca tatcttacct acagatgtaa aaacgatgcc tcatcctggt ttccctacag
     1261 atatgcaagc tcaaatgaca gctatccaat tagttgcaga aggtacaagt gtggtgactg
     1321 aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg aacgctcatg
     1381 tcaaaatcga cggaaatgta gcaatcatgg atggaaacca tgaattgcaa ggtgcagaag
     1441 tatatgcaac agacttgcgg gcagcagcag cattagtttt agctggcttg aaagcaaatg
     1501 gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg atattataat ttccatatca
     1561 aactacagca attaggtgca gacgtcgaac gtgtagacat ggatcaaact tctgctgaaa
     1621 aaacagcaca aacgattgct taaggaagtg gctgaatgag taaccaacga tatatcttat
     1681 taactttgat aaagatttta gtggtgatct tattgcttat ccttcttttt gtggccggaa
     1741 cgatgatcgg atatggtgtc atcggtggcg gcaatccatt taaagtattc cagccaagtt
     1801 tgtggattca tattcgagac ttttttcatt gataaataag aatcacagtg gttctagtcg
     1861 atatgaagtg agccatgaag cagaacatga taaaagaaaa cagaagggaa cgttgacgtt
     1921 agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc taattgatga agaagatatt
     1981 gatttattta gttagaggat atcagcgctt tatttcgcct gcatttccac ctagctgccg
     2041 ttattaccca acctgttcca attatatgat aaaagcaatt caaacccatg gtgcgctaaa
     2101 agggacggta atggggactg cacggatctt acgctgtcat ccttttgtca aaggcggtat
     2161 cgattatgta ccattgacat tccgattaac gcgaaataag aaagataaaa aagaaccaga
     2221 ttataaaaag gttgtgaaaa atacgggtag cactgagcga taaagaaaaa gaggaaattt
     2281 attgtcaaag taacaactcc gcaactactt atatggggga caaaaaatga tcgaagaact
     2341 aaacaagaaa gatttaccat ggtccttgtt attagatgca gatcctgata aagaaaaagt
     2401 tcaagtatat gtttccagag ggagtggctg gatatggaaa gaaaaagaaa aaacaatagg
     2461 tgtactgatc tatgtggcaa gggaaaaaga atacgaaatc gtcaatgtag cagtaacccc
     2521 aacgtatcaa ggaaagggga ttggcggaaa attacttgag acagcttttc agaagctttc
     2581 acagctagct tcaaaccaaa ctcggatcat tatacgtact ggcactacta gtagcgctgc
     2641 gttacacctc tatcaaaaaa tgggctatgt ggagattggt cgagtcaaag actattttat
     2701 ccatcattaa tctgagccca tttttgaaaa tggagatcaa ttaagggatc aagtgatttt
     2761 agcaattcac ttacctaaaa atagctttaa ctaataaact ttcaattcga gaaaatgctt
     2821 tcaaatgcta aatagataca ttacaatagc tttagcaaac aaaaaggaga atatttttat
     2881 gggaatatta gtttttgatt ttggtggatc ggctgtcaaa tatggttgtt gggatggcaa
     2941 agagattaaa ggtaagggac aatttgcaac gcctgaatct tgggaagaaa tgaaaacgca
     3001 attatttcaa gtttataaaa aaatactcgt ttcttttgaa gtcgaaggaa ttggaatcag
     3061 ctctcctgga gtagtgaata atgagaaaca ggtgattgag ggaatcagtg cgattcctta
     3121 tattcatgga tttaatattt tccgagattt ggaaactctg tttcaactcc cagtaacgat
     3181 tgaaaatgac gccaactgtg ccggaatggc tgaattttat gaaggtgctg cccgtgatta
     3241 tcaagatgtt gcttttgttg tggcaggaac tgggattggc ggggcgttgt tcacgaaagg
     3301 acaaataaat aaaggggcac atctttacgg tggagaattt gggttgatgt tcttagatgg
     3361 cgataagaca ttcagtaaac ttggtacagc ggtacagatg gcttggcgtt attgtgaacg
     3421 caaaggtctc gataaaaatg cttataccgg aaaagatgtc tttgaattag cagaaactgg
     3481 agatgcaata gcaaaagaag aagttgagag tttttatacg tatcttacaa agggattatt
     3541 cagtattcaa ttttcgtttg atccagaagt gatcgtatta ggcggcgggg tttctgcaaa
     3601 agaagggctc attgatgaga tcaaaagccg aatgaagaag ctgacagaac aattcgatct
     3661 gcatgatttc gaaccgcaga ttttattatg tgagtacaga aacgatgcta atcttgtagg
     3721 tgcagcagca aactatctcg ctgtacgtca aggagagaat taagagcttt agagaaggtg
     3781 cagcttgcat aaaagaagag aatgaaatga ataggagatt tttacctaag gaatgtttta
     3841 tttgctgcag tatgataaaa tatctgagag gtgaaaatga tgtcaaaaaa aaataaagat
     3901 attgaagtgc gtatagaaga agtgaaaaaa gatatcaaag gtaagaatta tgaagtcact
     3961 cagttattta tagggaaaaa aatgattggt gagatcttag cctatgggcc aaaagaatat
     4021 gaaattttct ttggtgaaga agattttgga aaagaaaaaa gtcttgaaaa tgcgatagaa
     4081 acagtgattc gacactggaa tctgcacgaa taaaaaaata tcaaaaaaac tttcaataaa
     4141 gggttgcaaa aatctggaaa attaggtatt ataactaagt caggttgata caaacctttc
     4201 ggaggagtag cgaagtggct aaacgcgacg gactgtaaat ccgttcctta gggttcagtg
     4261 gttcgaatcc actctcctcc atttcattgg ggtatagcca agcggtaagg caagggactt
     4321 tgactccctc atgcgttggt tcgaatccag ctaccccagt a
//
LOCUS       DACCVU010000171         4353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCVU010000171 DACCVU010000000
VERSION     DACCVU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.173"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            129..1907
                     /gene="uvrC"
                     /locus_tag="IVW29_002795"
     CDS             129..1907
                     /gene="uvrC"
                     /locus_tag="IVW29_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7840232.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            2052..2951
                     /locus_tag="IVW29_002796"
     CDS             2052..2951
                     /locus_tag="IVW29_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7840233.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            2944..4206
                     /locus_tag="IVW29_002797"
     CDS             2944..4206
                     /locus_tag="IVW29_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7840234.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
BASE COUNT         1486 a          706 c          916 g         1245 t
ORIGIN      
        1 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
       61 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
      121 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
      181 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
      241 aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta
      301 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
      361 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
      421 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg
      481 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
      541 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
      601 agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc gacgtagatc
      661 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa
      721 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
      781 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
      841 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga
      901 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
      961 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
     1021 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
     1081 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
     1141 ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
     1201 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
     1261 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
     1321 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
     1381 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
     1441 cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
     1501 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
     1561 ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg
     1621 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
     1681 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
     1741 cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt
     1801 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
     1861 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
     1921 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
     1981 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
     2041 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
     2101 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
     2161 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
     2221 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
     2281 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
     2341 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat
     2401 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
     2461 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
     2521 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
     2581 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
     2641 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
     2701 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
     2761 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
     2821 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
     2881 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
     2941 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
     3001 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
     3061 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
     3121 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
     3181 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
     3241 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
     3301 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta
     3361 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
     3421 gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac
     3481 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
     3541 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
     3601 acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg
     3661 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
     3721 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
     3781 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
     3841 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
     3901 tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
     3961 attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat tcgcttagca
     4021 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
     4081 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa
     4141 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa
     4201 gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt
     4261 tgtatagacc tattttattt attttactat attggatata tattcattga aaatagtgta
     4321 taacaacttt ataatattga gtataccaat ttt
//
LOCUS       DACCVU010000172         4339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCVU010000172 DACCVU010000000
VERSION     DACCVU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.174"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(119..490)
                     /locus_tag="IVW29_002798"
     CDS             complement(119..490)
                     /locus_tag="IVW29_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7840235.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(588..2201)
                     /locus_tag="IVW29_002799"
     CDS             complement(588..2201)
                     /locus_tag="IVW29_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP7840236.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEVGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAQIKGILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIEKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALKKAENRQNEIDCLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITDLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            complement(2313..2480)
                     /locus_tag="IVW29_002800"
     CDS             complement(2313..2480)
                     /locus_tag="IVW29_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP7840237.1"
                     /translation="MDNAVKTIQKKNNKCGIGNRGKTKIVVKEHFSEKGKTMEELLTD
                     VMLEKAKQTIA"
     gene            2613..2852
                     /locus_tag="IVW29_002801"
     CDS             2613..2852
                     /locus_tag="IVW29_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840238.1"
                     /translation="MAVREYHYQTGQVMAIKEDKSFTRTQIGCRSLLQGKGHGVSVSM
                     AYCLSNVLNCFIQLSKNTKEENRLSYIPRWKRAKA"
     gene            complement(3242..>4339)
                     /locus_tag="IVW29_002802"
     CDS             complement(3242..>4339)
                     /locus_tag="IVW29_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7840239.1"
                     /translation="KGKQPLNNELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDAL
                     KIIRDVLQLKADYMLYEDFGKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQME
                     SYLLAQERSWYDFMLGCMTAGGKLKRLDLAINDKAGILDIPKLIEKYKAGECISYFRK
                     QKDYGGTEKNGYDTPQNTGETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNR
                     FEIRMKNERAYYAVVDLLTYRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWF
                     VGENREPIRLTTKPEPYTLQKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAEL
                     KDKHKHLLQLEKSTIEERIDTVVPQENDGIF"
BASE COUNT         1155 a          949 c          663 g         1572 t
ORIGIN      
        1 ttcatctcta tctataatta acattaaaaa tagtgttttc acattatttc tcaaaccaat
       61 cttaaaaatc atcaggagta cttttttgat atacttttat taatgctggg cgtaatggtt
      121 aaaagatttc cactagttcc tctccaccaa gctgagttga aaaatgctta tctgtaaaca
      181 acatagacaa agaggaaata aacgtttccg tatctttctc tgtcccaagg atattaatta
      241 attttcctac taggtaagta tcattaaact taagatctgc cttcacatca aaagttttga
      301 taaaagcttt tatcatgcgt acaattttgt ctttatcgcc ataattgatt tcaccactca
      361 tatcaatata gtcactaatc cctgaagaaa taatggcaac tgcttcttct ttcgccttat
      421 cagataattc atttaatgac tgggattgat tgatgacttc ttgagtttta tctgttacgt
      481 aatctctcat tatcctatcg gcatactccg caaagtaatc ttgaaaaaat tcttctacag
      541 ggtaaccaac ttccgtctcc cttagttaaa gatatgaacc ctatttatca gtctactttt
      601 ccaataaatc ggtagtatat ctctatttcc tgtattcttt cgttctcctc attcgttgtt
      661 gcttcgtgaa tgagtatttt ttctatcatt gcatttaata acggtgctgt cagttccttt
      721 ggtacggaat actccttgat taactcaatc catttttcag cacctatcat atcctgttcc
      781 attttactta attcttctct taagtcggtt atctgctgtt ccagttctat ctgttccttt
      841 tgatattttc ccgacagcat aatgaagttt cgttctgtta tcttctcaca ggctctatct
      901 tcatacattt tcgcaaataa acagtcaatc tcattttgtc ggttctcggc tttcttcaag
      961 gcacttgcct ttttcttctt ttcccgtatt cgctcggcat taccgacttt ctgtatctta
     1021 tgcaatacct tttcttcgtc ctgctgtact gccttagccc aatactgcaa tcgctccaat
     1081 acagcttgat acagcacatc atagcggata tagtgcatag aacaatagcc tttgccaaat
     1141 tgtccataga aactgcaagc ataataactg taaggcgtct tatttgcctt atttgttgca
     1201 aatctcatag accagccaca atccgcacac ttaacaagcc ctgcaaatat cggcgttgcc
     1261 ttttcctttg tctgtctacg ccttgatttt atctgttcct gaacacgata gaacacttct
     1321 ttttcaataa ttggctcgtg ggtattttct actcgaaacc attcgctttc gggctttcgc
     1381 actttcttct tgcttttgaa tgatactgtt gactgtcgat tatgaacgct gttgccgata
     1441 taaacttcac ttttcaaaat tcctttgatt tgtgcaatcg tccactcata acgcttactt
     1501 tcgggtttgc cctcaaaaat atgtgcaaaa gtaccatacc ttgtaaaatt caaccaagac
     1561 gatgtcggga ctttttcttc tcgcagtatt tttgtgattt ttgcactacc gtaaccttga
     1621 taggcgagag agaagatttt ttcaacaatc cattttgttt cctcatcaac caacagtttc
     1681 cctttgatgt cggggtgttt cttatatcct aacggagcat aagccccata atacgcaccc
     1741 tctgcaaatt ttgtcttaaa ggctgatttc actttacggc ttgtatctct tgccacccat
     1801 tcattgataa tattcttaaa tggtgcaatc tcactttcgc ctttttcgct gtctacaccg
     1861 tcgtcaatcg ctatgtaacg gacattatgg ctgggaaaat acaattctgt atattgtcct
     1921 gtcataatat agtttctgcc aagtcgggaa aggtcttttg ttacaacaca gttgattttt
     1981 cctacctcta tatcctcaat cattctctga aaactcggtc tttcaaaatt tgttcccgac
     2041 cagccgtcat caatatattc atcaatgaca ttcaaatgat gttcttttgc atatagacgc
     2101 aacatacttc tttgtgtggt aatgctggaa ctttcgcctt gtaattcatc atctcggctc
     2161 aacctaagat aaagtgcagt attgtaaatc tgttgtttca ttgtcagtta tcctccttta
     2221 taactaaccc gtgtttacaa tacctgcttg caagtaaagt ataacacggg tactttttgc
     2281 cattcaatct atttcttaca actgcaccga atttatgcga ttgtctgctt tgctttctca
     2341 agcataacat cagttaaaag ttcttccatt gtctttccct tttcggaaaa atgctctttt
     2401 acaacaatct ttgtctttcc tctgtttcca atgccacact tgttattttt cttttgaatt
     2461 gtttttacag cattatcaat aacacatacc cccctttctg taaaatcttt ctaagcaatt
     2521 caacagcttt tgtcatagcc cacaggagtt ccacctctgc attatccaat gtagccgtac
     2581 ccattgcgtg cgacgctttt aacgctcaag ctgtggctgt acgggagtat cattatcaga
     2641 caggacaggt catggcgata aaagaggaca agtcttttac aagaacacag ataggttgtc
     2701 gctcgctttt gcaggggaaa ggtcatggcg tatctgtttc aatggcttac tgtctgtcta
     2761 atgtattgaa ttgctttatt caattgtcaa agaacacgaa agaagaaaat cgtctttcct
     2821 atataccgcg ttggaaaaga gcaaaagcgt aaccaatact caaaactttt ttctttttct
     2881 gtaccatata gggaaagaaa atgtcattct gctaagttga tgatgaaaag atttcttttc
     2941 acaccctaac tacccatgca acaccgttac tgccaactat ggaatgaaag attttctttg
     3001 ctccatatag ggaatggaaa ctgctgattg ttaagttgag aatgaaaaaa atttcttctt
     3061 ttcacataac actgtccgct taactcgatt ttccgacatt tttcaaaact tttcttcttt
     3121 cacttcatat agggaatgga aacctcgatt taatgaccta ttttcaaaaa agtttagatt
     3181 tccttttctc gtaccatatc tgtacgctag ttgatgtttt gttgcagatt ttttaaagaa
     3241 attaaaaaat accgtcattc tcttgtggaa caacggtatc tatgcgttct tctatggttg
     3301 atttttctaa ctgtaataaa tgtttatgct tgtctttgag ttctgcctgt tcaatcatat
     3361 cttttagtat cgttgtatga ttttctttgt ctaatgcctg taccattttt atggttggtg
     3421 caacctgtct ttgtagccaa tgcaatgctt tttgtaaagt ataaggctct ggctttgtgg
     3481 ttaagcgtat cggctctctg ttttctccta caaaccacgc ccaatcatca ttcattttcc
     3541 attgactttt cggtttatcg tcctctctat ctacaaaacg gacataatga ttgatgatag
     3601 aaaaagcagt acgttcagcg tcccgataag tcagtaaatc tacaaccgca taataggctc
     3661 gttcattctt catgcgtatt tcaaaacggt tcttgatttc tgtatcttca acttctgtgc
     3721 cattcttgac atactgctca taattcttct gataagcaca catatataat tcgctacttg
     3781 ttgaaccgag atataaggtt tcgcctgtat tttgtggtgt atcataccca tttttctctg
     3841 taccaccgta atctttctgt ttgcgaaaat aggagataca ttctccagcc ttatattttt
     3901 ctattagttt tggaatatcc aagatacctg ccttatcatt gattgccaag tcaagccgtt
     3961 tcagcttacc gcctgccgtc atacaaccaa gcataaagtc ataccatgag cgttcctgtg
     4021 ccaagagata actttccatt tgccgacacc ctctgccttt cagttccaac aaaacaccta
     4081 aatgctcttg catggaacac atgatgttaa tgtcgcctaa aacatattta ctttcatatc
     4141 cgtactttcc aaaatcttca taaagcatat aatcggcttt tagttgtaat acatcacgaa
     4201 taatctttaa tgcgtccgta gtaggaaagc ggacacgaaa gtaatcaatt agtaaaaata
     4261 acggctctcg tggattgaaa cgctcaatct gtttttctat gatttccttt aattcgttat
     4321 tcaatggctg tttgccttt
//
LOCUS       DACCVU010000173         4295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCVU010000173 DACCVU010000000
VERSION     DACCVU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.175"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..792
                     /locus_tag="IVW29_002803"
     CDS             <1..792
                     /locus_tag="IVW29_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7840240.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="IVW29_002804"
     CDS             889..3138
                     /locus_tag="IVW29_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7840241.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="IVW29_002805"
     CDS             3141..>4295
                     /locus_tag="IVW29_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7840242.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DACCVU010000174         4268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCVU010000174 DACCVU010000000
VERSION     DACCVU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.176"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            533..1501
                     /locus_tag="IVW29_002806"
     CDS             533..1501
                     /locus_tag="IVW29_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7840243.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1512..2327
                     /locus_tag="IVW29_002807"
     CDS             1512..2327
                     /locus_tag="IVW29_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7840244.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2395..3105
                     /locus_tag="IVW29_002808"
     CDS             2395..3105
                     /locus_tag="IVW29_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840245.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3141..>4268
                     /locus_tag="IVW29_002809"
     CDS             3141..>4268
                     /locus_tag="IVW29_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7840246.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1431 a          638 c          901 g         1298 t
ORIGIN      
        1 tgagacaaaa caaaatgacc tcttttattt aagaaaggag gtcatttttt gtatcataaa
       61 ttgtattaaa atcaatgtat cactttttaa tcagttttaa gaccataaat tgcgtactaa
      121 aagaaattct gcaattcaaa ataaagcatt ttaagccatt ttcaaattta agtgcaccca
      181 tctacccgtc aaatcacttt attttccact ccagtagttt cctaattcaa ctgtgtgcaa
      241 aaacgatttc ttctaaacaa atcaagacaa acttataaac cttcatccca gcaccataat
      301 tatttaaatc ataggcattt ttcgaaatta gtatatgtac tcaagtttca ctgcagatta
      361 tttatatttt ttagagaaat aaatttttcc tattgacaaa cacaaagaaa gcggatacaa
      421 tgcaattatg tcacatgacg ttatatgacg ttatgtgata tctttacatt taatcagtta
      481 cttttatttt ggataaaatt aacattttta aaattttaag gaggaaatgt tgatggatgt
      541 agcaaaaacg gttaacgcaa ttgtagaaaa aaatgatgga gagatttctg gaatttattt
      601 ggtggcttgt ggcggttcgc tggtagatat gtatgtctca gattattttc ttaaagccga
      661 agcatcgaag tgtattacag gcttatatac agcgaacgaa tttaaccatg taaccccaaa
      721 gcaattagat gataaatctg ttgtcattct atgttctcat ggaggaaata cagcagaaac
      781 ggtaaaagca ggagaagtcg ctcaagctag aggagctgtg actgttggtc tgactcataa
      841 taaaactgcg gatttattaa aggtttctga tcatagtttc ctttacgaat ggggagatga
      901 tgcaaaaata gtcaataatc ctatggccat cattcttgat ttaactgtat ctttggtcaa
      961 tgttaccgaa acatttgaac attacggtaa attcagagaa ggcatgaagg ttatcgatac
     1021 cgtaattgaa aatggcatta agcaggtgca ggatcgtatt aaagtgtttg ctgataagta
     1081 caaagatgaa acaatgtttt atatcttagg tagtggacca tcatatggac atgcttatgg
     1141 tttttcaatt tgctctttga tggaaatgca atggcttgat tcaacttctg ttcattctgg
     1201 agaattcttc catggacctt ttgaagtaac agacaaagat acgaatttta ttctcttgat
     1261 gagcattgga cgcactcgtg ctttagatga acgggcaaaa cgattccttg ataaatatgc
     1321 agaaagagta gaaatcgttg atgcaaagga actaggattg gatatgatat cagatgaagt
     1381 ttctgaattc ttcaatccca ttttattcta cagtattctt tcagaatatc gttcagcttt
     1441 agcagatgta cgcaatcatg atttagatgt tcgtcgctat atggggaaag tggagtacta
     1501 ggaggattaa aatgaaggta ttaggcttag gtgataacgt cgtcgataaa tatgaaaatc
     1561 tgggaattat gtatcctgga ggaaattcat tgaattttgc agtttttgca aaaaaactgg
     1621 gacatgaaag tagctttatg ggagtttttg gctcagatga ggaggcagct catgttttat
     1681 cagcaataaa agaaatagga ctggacaaca gtcactctcg ctttgaaaaa ggcgagaatg
     1741 gctgtgccag agtgaagatt gatgagggcg accggatttt ccttggcagc aattcgggag
     1801 gtgttactcg tgaatacccg attcagctta ctgaagaaga cagagaatat ttaaaccagt
     1861 ttgaattgat tcatatggga ttatatagcc atgtcaatca tctattagaa gagttacaaa
     1921 atgtttctgg aaagatttct tatgattttt ctgacgactt tacggaagaa gaggttcagc
     1981 gggcaattga taaagttgat tttggcttct tttctataga agacttttcc gatgaagaag
     2041 taaagcagtt tttgatgaag catttttgtg agcgaaatga tgtattgatt gcaacccgag
     2101 gagaaaaaac ggctattggt tatgatggag caaagtttta tgaagtgaac cccgtgctag
     2161 aagaaccagt tgatacaatg gcagcgggag attctttctt aacagctttc ctactttatt
     2221 ttctcgaagg ggacgatata gtcaccgcga tgaaaaaggg gaatgaattt gctgggaagt
     2281 cttgtctggt tgacggttcg ttcggctttg gtattcatta cgattgagct tgttttaaat
     2341 tcctgcattt gataacctaa agaaagaaat cccggtaaag tggaggttaa aaaaatgctg
     2401 gatacaaatg cacaattacc attgtactta caattaaaag aaatattaaa aaataaaatc
     2461 aatacagggg agtatcccga gaatacgaaa attcccgctg aatcagagct gatagaaagt
     2521 tatgaggtca gccgaatcac agtgagaaaa gcagtagaag aactggtaga agaagggtac
     2581 ctacttaaaa aacaagggaa aggaactttt gtcaaccggc ataaagtttt tcgtaaaatt
     2641 gaatatatta tgggtttcag tgagagttgt aaatctaatg gttttacacc gactagcatt
     2701 gttttggaac gcaagatcat tccagcaact gaagggatgg caaaaaaact cgatatcgct
     2761 caaggtgatg atgttttata tatccagaga aagcgaatgg cggataacat ccccattatg
     2821 ttagaaaaca attatttttc ttatgaaaga tatagtttct taatggagga agagctgact
     2881 gattctttgt atgaagtact taaagtccaa ggtaccttag ccatcaatcc tggggaaact
     2941 gcacttgaaa ttgtcacagc tgatgaccaa attgccaaaa taatggaagt agctgttggg
     3001 acgccctttt tctttatgga tactttaatc aacgatcaaa acaacatacc aattcatgta
     3061 ggtaatcaat attacctggg agaagtatat cgtttttact tatgatttcc tgtttggatt
     3121 tcaaggagta gattttattt atgaagcaaa tattgcggtc aaaaatgatt tttgatggag
     3181 agcagccacc tttcaaaggt tatattgtaa tcaatgaagg gatcattgaa aaggtggaaa
     3241 acaactggaa ttacagtgat ctattaaatg aaaacacgga attaattgct tatgatgatc
     3301 aattcgttat gccagcaatt catgataatc atgtattttt cagtggttat ttggctatga
     3361 atgctggcat agatttaagc gatacatctt cagctaacga ggctgtagag cgtattaaag
     3421 ctgtaatgaa ccataaaaaa gcagatgaac caatttatgc tcatggctgg gatgctgccc
     3481 tgtggaaaac tgacccatca gaagagctat tgaatgaaat aaaccacttt ggtccaatca
     3541 cagcgatcga caaaaatcgc agccattgtt ggatgaatgc tattgctaag cagacttatg
     3601 gttttacaga aaaagaaacc agtgctgaaa gtcgagtatt attaattaag gaaatgctag
     3661 caaacaaaaa gcttttagca gaggtttacg ctgaatttga agcattactg ctttcaaaag
     3721 gtgttatatc aatgaaagaa atcgtttttg atgacgcaga atttctttct aatatacctc
     3781 agaggaaaat acttagtaac ctatatgtgc aggcagtcca agaacctcta cagcatgaac
     3841 ttctatatga gtataaggaa aaagttttcc ctgaaaaggt ccgctttggt ggagtgaaga
     3901 tcatggtaga tggtgttgtt gcggatagaa gtggagatat ttacggtgtt tatcagtcag
     3961 gagtagcttc acccgagatt gactatgagg caattaaagc agaagttgct gcattcaatg
     4021 aagtagagat tccttgctgt ttgacaacgg aaggagataa agcgggttat aaagctgcgt
     4081 caattcttgt cgaatttgga aagcggttac ctgaaggcgt ttttaattcg attagtgatt
     4141 tggaaatggt tactcaaaag acagcagaaa aaatgaacga aggacaagta gtagcagagg
     4201 tatatccaca aattctcggc ttgaatcaaa cgttagaaga ggcttacatg tcaactgtta
     4261 taaaaaat
//
LOCUS       DACCVU010000175         4093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCVU010000175 DACCVU010000000
VERSION     DACCVU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.180"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            61..1485
                     /locus_tag="IVW29_002810"
     CDS             61..1485
                     /locus_tag="IVW29_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7840247.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1666..2439)
                     /locus_tag="IVW29_002811"
     CDS             complement(1666..2439)
                     /locus_tag="IVW29_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7840248.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2436..3341)
                     /locus_tag="IVW29_002812"
     CDS             complement(2436..3341)
                     /locus_tag="IVW29_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7840249.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3495..3815)
                     /locus_tag="IVW29_002813"
     CDS             complement(3495..3815)
                     /locus_tag="IVW29_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7840250.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1235 a          753 c          819 g         1286 t
ORIGIN      
        1 aaataaaaaa ggtttgttat actttttgat aaaagcggtt ttattttgga ggatgcttac
       61 atggatacag agcaacagaa aaaaggaatt ggtttaattc cacttgctgc cttagtaatt
      121 ggttcagcga ttggaggtgg cgtatttggt attatgacgg acatctccgg atcggctggc
      181 ggcccagcta ttttcagttg ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg
      241 atcaataata tcaataaaaa acgtccagat ttaaaaggcg ggatctttag ttacgcagaa
      301 gaaggttttg gccgtttcgc tggttttatc agcggttggg gttactggct cacatgctgg
      361 ttaggcaacg tagcttttgc aacattgttg atgagcgcat taggatattt cttcccagta
      421 tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt atggggatat
      481 gcatggctag tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat cgtcacattc
      541 tgtaaattag tgccattatt tttatttatt gtagtagcta tcagcacgtt ttcgatccaa
      601 caattcattg ataacttttc tagcgacatt atattgaatg cgaatggaca gccgatcccg
      661 ttttccagtc aagtattgag ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa
      721 ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa agcaacgatt
      781 ctagggatat tcggcttgat tattatttac gtcttggttt ccatgcttcc atatgggaca
      841 atgacgatga atcagctgca aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa
      901 atgatggttg gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat ttctttagtt
      961 ggtgtttggc tctcatggac gatcttaccg attgaaacaa tgggaaacat ggctgaagac
     1021 ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac ttatgctatc
     1081 gtattgacga cgttatgtac gaatgtcttt ttactttcat tattattcac tgacagtgct
     1141 tataactttg cttacacact tggtacagca gctattttct tcacgtggct tttcttagga
     1201 ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg tccaattttt agtagggctt
     1261 ttagcaagtg cctttcaagt atgggctatg ctagtcgtag cctttaatga agtaatgctt
     1321 gttcttgttc ttttcttgcc aggcatttat ttttacctca aagcacgtaa ggagcaaaac
     1381 gagcctgtac aacatatcca cttagataag attttactta taggtgtgac gatggtcgga
     1441 gtgattgcaa tggttttatt tgccactggg atgcttcacg tatagaagta taaaagaagt
     1501 ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag gagctgtgac
     1561 acaaatgttg tcacagctcc tttttcagaa taaacggtgt ttaagagaca gccaatcggc
     1621 aataaggctg aacgtctctt tcacagcctc ttttttgatg agtcatcata cattttttcg
     1681 cttaaatacg agatatccta atgctaagaa caaaacgatg taagctaaat taccgaatag
     1741 catttgatta gtagaaagat gagtcaattg cttcatgatc tcttcgtttc caatttggtt
     1801 ttgcagattc aacatactga tcggattcca tttgatccac tcccattttt ggatgagtgc
     1861 aataaggata ccagatatga ttgacgaagc aaaataaaag acgattcctg ctgagatgga
     1921 tgcgccggaa ctattgataa agcaagcaat catcaacact aaacttaaaa tcagccataa
     1981 accgatataa ctacccaaag tataagtgaa caagctttgg atcagtgttt gaccagtaga
     2041 aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat
     2101 aatagctaaa aggtataaat aaacagaata aataactaat gtgatccatt tgcttactaa
     2161 tatttctcct cgagaatact ttcgataaag gagatttttc aatgtaccat attgtgcttc
     2221 catcgaaatg atcgtgcttg cagctgcaat catgataaag acaatccaag atgtggcact
     2281 aaaaagctga gcagtcattt caactggatc aatgatcgtc gtgtattttt tggctagaag
     2341 tgctgttcct atgagcaaaa caactaatag aacagataaa acagcagttg actttttctt
     2401 gatcaattta aataattctt gttttacaag caatgtcatt tttcttcttc cccctttact
     2461 gaatccagaa tttccaatag agaagattcc aaatcattcg tcagatgctg gacgtctgtc
     2521 acatggatat tttgtgcaga caatagctgg atcacggttc ctaataattc ttcgttttct
     2581 tttaaaagct tgattttatc aggatcttcc aaagtatatc cagcttctgt caagatcgtt
     2641 cgagcagcta gatcatttgt tgtagagata acgatgaaat cttgcgctgt ttggttcaat
     2701 tcagccattg tacattgttt gatgatcttt ccttgattga tcaggatcag ctcatcgacc
     2761 attttttcaa gctcgctgag taagtgactg gagatcaaga aagaggtgcc ctgagtagcc
     2821 aagtcggtaa tgatttttcg cacatctctt gttgcttgtg gatccaaacc gttcatcggc
     2881 tcatctaaaa taacgataga cggatggttc agtaaggcaa gagcaattcc caatttttgt
     2941 ttcattccca acgagtagga tttcgctttt ttgtgaatgt acttttccat cttgagctga
     3001 ttcacgatag tgtcgatagc agatacatcg ttggtttcag agaataactt cagatgatca
     3061 tagcctgtta agaatggata gatacccggg tattcgatca atgctcctac ttgttccaac
     3121 cctttatgac tagagggaga aatctcattt tcaccaatac agattgttcc atctgagtaa
     3181 tggatcagtc ctaaaatagc tttcatgatc gttgtttttc cagcgccatt aggacctact
     3241 aatccaacga ttttgccagg ggataagcta aaactgacat cgctgagggc ttgtttgtga
     3301 ccaaagttct tggataattc ttggaccgaa agacttttca aaaatattca ctccttttct
     3361 atagatagta caagcataca cctgatgagt ggagaagtcg ctaggtcatt ggacttattt
     3421 cttatttttt cataaaaaaa ttgaatatta aataaaaagg cttatttttt ctgatcagta
     3481 cttattccaa gtgtctagtg gccgctagta atcttgatgc caatgatact gatgagtagc
     3541 atccccacaa agaaccaagt ggaaagattc agcttatcat gaaacaagac cacaccaatt
     3601 atgaccgaac caactgcgcc aatccctgtc cagataggat aagagagact catcggcaaa
     3661 cttttgattg cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa
     3721 gaataattga ttttagaaaa accatcactc catttcatca tcgttgacca gaatacttcc
     3781 aaaattcctg ctatgattaa ttccatccat gccatactta cattcctcct taactataaa
     3841 aaaatacaca aaaagaggaa aatcatccct tcaaagacct aatggaatga tcatcctctt
     3901 tatcctaccc ggtggcagat tgtatatctt tttctgtagt atagcacacc tatccagata
     3961 tcggcaatct catacttttt agtaaagtgt accaagcact gacggatata tcgttagcgg
     4021 aaaagaagat gaatgctttg caatttcata gaaagaagtt gttatttatt aatcatttaa
     4081 aaaaaacagt aac
//
LOCUS       DACCVU010000176         4037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCVU010000176 DACCVU010000000
VERSION     DACCVU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.181"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            854..1066
                     /locus_tag="IVW29_002814"
     CDS             854..1066
                     /locus_tag="IVW29_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840251.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="IVW29_002815"
     CDS             complement(1344..3143)
                     /locus_tag="IVW29_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7840252.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="IVW29_002816"
     CDS             complement(3267..3863)
                     /locus_tag="IVW29_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7840253.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          688 g         1189 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgta taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg ttttttt
//
LOCUS       DACCVU010000177         4017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCVU010000177 DACCVU010000000
VERSION     DACCVU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.182"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(130..1635)
                     /locus_tag="IVW29_002817"
     CDS             complement(130..1635)
                     /locus_tag="IVW29_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7840254.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1632..2318)
                     /locus_tag="IVW29_002818"
     CDS             complement(1632..2318)
                     /locus_tag="IVW29_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7840255.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2511..3932)
                     /gene="gndA"
                     /locus_tag="IVW29_002819"
     CDS             complement(2511..3932)
                     /gene="gndA"
                     /locus_tag="IVW29_002819"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7840256.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1105 a          852 c          690 g         1370 t
ORIGIN      
        1 attaacggat gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat
       61 ttctcacttg cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa
      121 gcacatgctt tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc
      181 ttgccctact acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc
      241 gatagacaac cccagaccat ttccaccctt cgttctagct cttgcattat ccactcgata
      301 aaaacgattg aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat
      361 cgcgatctca aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt
      421 tgaatacttt actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata
      481 aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata
      541 taatatcttg aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt
      601 cgcataatga acatctactt gttctgctct tgataagtct agcatctctt gaactaagct
      661 tttcatacgg ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc
      721 atctttcccc caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct
      781 taactcatga gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg
      841 gtcaagcatc tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc
      901 aggcatgtgg atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac
      961 cttgagtggt tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa
     1021 agaaacaact tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga
     1081 agataattca tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat
     1141 aggctggata gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg
     1201 tgtagtctga atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa
     1261 atcataaaca taaagatcca ttgatggctg ccctagctca gctataaagg aatcgatctt
     1321 cattaagttt ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc
     1381 tttcgtcaga ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg
     1441 agaagctact tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt
     1501 gatagatgat ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga
     1561 actcgcaaag gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt
     1621 tttcggcttt atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt
     1681 tcctccccgg gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt
     1741 gtttcaactt ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc
     1801 acgttcacgt tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt
     1861 acttcatttc cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta
     1921 atagtagttt gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca
     1981 cgaagacgtg ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg
     2041 tggtcaagtc cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt
     2101 gtatttttga cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc
     2161 agatccaaca atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg
     2221 ttgtagtgga cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc
     2281 aagttctttt catcttcaat gattagaata ttgctcattt attattttat cctttctatt
     2341 ctactggctt tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca
     2401 ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca
     2461 gctctttcgt cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca
     2521 tggtaccatg agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca
     2581 aagtaatcgc gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg
     2641 aagtaagcga tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca
     2701 acgacgtctc tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc
     2761 aataaatttt cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga
     2821 gctcggatga tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa
     2881 tcaaattctt ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta
     2941 ctgaaataca acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca
     3001 aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa
     3061 atatagcggg caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg
     3121 ctttggcttg tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact
     3181 actggtttac ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc
     3241 aaatagctgt ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact
     3301 gaaaggttta aaatattctt cattaagtca taagattcag caatcagttg catatctcca
     3361 tactcaatac cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat
     3421 gtgacacatg gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact
     3481 agcgcatagg cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct
     3541 tcaccaccgg aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta
     3601 cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca
     3661 aggtgaggaa gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc
     3721 aagatccgac gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg
     3781 aaatttttat ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat
     3841 aaagccactg aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc
     3901 ataccgacaa cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct
     3961 ccctaatctc ttacattata gcttaacacg gctacattgc aaactaaaga acaacat
//
LOCUS       DACCVU010000178         4017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCVU010000178 DACCVU010000000
VERSION     DACCVU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.183"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..515
                     /locus_tag="IVW29_002820"
     CDS             18..515
                     /locus_tag="IVW29_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840257.1"
                     /translation="MSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTL
                     IAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLC
                     LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYF
                     YRNKL"
     gene            complement(641..1318)
                     /gene="nth"
                     /locus_tag="IVW29_002821"
     CDS             complement(641..1318)
                     /gene="nth"
                     /locus_tag="IVW29_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7840258.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1325..2023)
                     /locus_tag="IVW29_002822"
     CDS             complement(1325..2023)
                     /locus_tag="IVW29_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7840259.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2291..3475
                     /locus_tag="IVW29_002823"
     CDS             2291..3475
                     /locus_tag="IVW29_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7840260.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3481..3924)
                     /locus_tag="IVW29_002824"
     CDS             complement(3481..3924)
                     /locus_tag="IVW29_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7840261.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1053 a          785 c          654 g         1525 t
ORIGIN      
        1 tgaaagaagg cttcattttg agttatctga tagtatgctt aattatcata atttgtctgc
       61 accatctttt tttatcgaaa acaatacctc gttattccaa gaataaaaaa gctgaaaaat
      121 tttgtttatt attaaataaa tttactttaa ttgctcctat ccttgcattt attatttttt
      181 ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga
      241 ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac
      301 cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca
      361 tttttattac acctttagat agatacgtca cttatttata taatcctttg gaatactgga
      421 cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa
      481 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
      541 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      601 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      661 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      721 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      781 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      841 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      901 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      961 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
     1021 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
     1081 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
     1141 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
     1201 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
     1261 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
     1321 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
     1381 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
     1441 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1501 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1561 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1621 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1681 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1741 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1801 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1861 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1921 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1981 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     2041 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     2101 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     2161 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     2221 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     2281 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     2341 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     2401 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     2461 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2521 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2581 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2641 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2701 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2761 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2821 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2881 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2941 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     3001 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     3061 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     3121 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     3181 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     3241 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     3301 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     3361 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     3421 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3481 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3541 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3601 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3661 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3721 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3781 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3841 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3901 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3961 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaacca
//
LOCUS       DACCVU010000179         3989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCVU010000179 DACCVU010000000
VERSION     DACCVU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.184"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            526..2019
                     /locus_tag="IVW29_002825"
     CDS             526..2019
                     /locus_tag="IVW29_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7840262.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2154..2450
                     /locus_tag="IVW29_002826"
     CDS             2154..2450
                     /locus_tag="IVW29_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7840263.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2551..3402
                     /locus_tag="IVW29_002827"
     CDS             2551..3402
                     /locus_tag="IVW29_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840264.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3423..3635
                     /locus_tag="IVW29_002828"
     CDS             3423..3635
                     /locus_tag="IVW29_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840265.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1417 a          577 c          805 g         1190 t
ORIGIN      
        1 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
       61 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
      121 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
      181 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
      241 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
      301 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
      361 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaaagt ttatttagtg
      421 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
      481 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
      541 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
      601 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
      661 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
      721 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
      781 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
      841 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
      901 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
      961 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     1021 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     1081 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     1141 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     1201 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     1261 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     1321 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     1381 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     1441 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     1501 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     1561 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     1621 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     1681 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     1741 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     1801 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     1861 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     1921 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     1981 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     2041 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     2101 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     2161 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     2221 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     2281 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     2341 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     2401 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     2461 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     2521 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     2581 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     2641 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     2701 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     2761 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     2821 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     2881 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     2941 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     3001 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     3061 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     3121 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     3181 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     3241 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     3301 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     3361 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     3421 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     3481 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     3541 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     3601 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     3661 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     3721 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     3781 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     3841 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     3901 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     3961 tcgtaagaga aattgatcga agatgtgac
//
LOCUS       DACCVU010000180         3918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCVU010000180 DACCVU010000000
VERSION     DACCVU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.185"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            117..1043
                     /gene="cysK"
                     /locus_tag="IVW29_002829"
     CDS             117..1043
                     /gene="cysK"
                     /locus_tag="IVW29_002829"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7840266.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1279..1725
                     /locus_tag="IVW29_002830"
     CDS             1279..1725
                     /locus_tag="IVW29_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7840267.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            1998..3350
                     /locus_tag="IVW29_002831"
     CDS             1998..3350
                     /locus_tag="IVW29_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7840268.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTVKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3521..3808
                     /locus_tag="IVW29_002832"
     CDS             3521..3808
                     /locus_tag="IVW29_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840269.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1371 a          627 c          822 g         1098 t
ORIGIN      
        1 taccatatat ggtatatctt tgtctttttt ttgctagtca aagacgaatc actcgccata
       61 aatgaacaga ttgtattatg ataagtaaga agagcaaaga caaggaggaa ttgaagatga
      121 cagctatcta taactcagtc accgaattga ttggcaaaac gccaatcgtc aaattaaata
      181 aaatcgtacc agaagattca gcagatgttt ttgtaaagct tgagtttttt aatccaggag
      241 gaagtgtaaa agatcgtatt gctttaagta tgatagaaaa agcagaacac gatggtctac
      301 tgaaaccagg tgatactatc attgaaccta cttctggaaa tacaggaatc ggattgtcga
      361 tggtaggagt tgcaaaagga tataaagtaa tcatcgtgat gcctgagacg atgagtatcg
      421 aaagacgttt attgatgaaa gggtacggcg cagagttgat tttgactcct ggtgcggatg
      481 gtatttctgg atcaatcaga gaagcagaac gtttggcaaa agaaaacggc tactttttac
      541 cattacaatt tgaaaatgaa gcaaatcctc ttgtgcatga gaagacgact ggtcctgaga
      601 tacatcaagc atttggagta aatggcttgg acgcgtttgt tgcaggtatc ggtacaggag
      661 gaacgatcac tggcgctggc cgagaattaa aacgtgtgta tccgaaaatc gaattaatcg
      721 gggtagaacc agcagaatct gctattttag aagggaaaga agcgggacct cacaaaatcc
      781 aaggaatcgg tacaggtttt gttcctaaaa cactggatac ttctgtgtat gataaagtcc
      841 tttctattag tggagacgaa gcgatggaaa cagctagaga agttgggcga aaagaaggga
      901 ttttagtagg catctcatct ggcgctgcaa ttgctgccgc gctaaaagtc gcaaaagaat
      961 taggaaaagg caagaaggta ttagctgtgg ttcccgacaa tggtgaacgt tatttatcta
     1021 cagcccttta ccaagagata taaggcactt ggtttttctc cagataggtt tatgtaaaaa
     1081 acttttaaag atgaaaagaa cacacaaaaa tttatgatga gaaaaattat ttgaatagag
     1141 acgtagaata gtgaacgcga ctgttttacg tcttttttca ttaaaataca ataaaattga
     1201 attttctttt catgttaagt ggattttaaa atgtaaaaat agtatgataa gacgagaata
     1261 acaacgtggg gtgtttacat ggaaaataag ttagtcgaag aggttatcga aaagttaaaa
     1321 aaagagaaca ttcgaatcac acctcaacgg tacgcggttt tagaatattt gattgaacat
     1381 cattcccatc caacggctga tgagatttat aaagcacttg aacatcggtt tccaaatatg
     1441 agtgttgcga ctgtttacaa taaccttcgc ttatttactt cgattggatt tgttcaagaa
     1501 atgaagtacg gcgatgcttc tagccgattt gatttcagtt caaagcgtca ttatcatgta
     1561 atttgtgaaa actgcggtaa gattgttgat tttcattacc cagggcttga agatatagaa
     1621 atggctgcaa gcagactgac tgattttgag attaatgaac atcggttgga attgtatgga
     1681 ctctgtccgg attgtaaaga gtcagcaaaa aaggcgcaaa tgtaaaaaca gtttctacaa
     1741 gtgttatacc taaaaaaagc tagctgtgta aaaaaaacgc tagctttttt atttttttgt
     1801 cttttattca caatcttaga tataaaagtg aatattatta aaataagaat cttgtagata
     1861 ttgggttctt atgcttgaaa aggaatgaaa ctacaaacaa acctttgtga attcacaagc
     1921 attaaactgt tgtatccgtt ttatttctgt tatagtagtt ttgtcgaagg acaaaatgtt
     1981 aatcggaggg accataaatg aaagtagtag taattggctg tactcatgca ggtactgcag
     2041 ctgtaaaaag tattttaaaa aatcatccag aagcagaagt gacagtatat gaaagaaatg
     2101 acaatatttc tttcttatct tgcggtatcg cactttatgt cggcggagta gtaaaggacc
     2161 cagcaggctt gttctactca aatcctgaag aattatcttc attaggagca acagttaaaa
     2221 tggaacacga tgtaacagac gtcgatacag aaaataaaac agtaacggta aaagatctga
     2281 aaacaggaga aacagaaacg gtttcttatg ataaattagt gatgacaact ggttcatggc
     2341 ctattatccc accgattagt ggaatcgatg cgaaaaatgt actcctatgc aaaaactaca
     2401 atcaagcaaa tgaaattatt gcacaagcga aagaagcaaa acgtgtagta attgttggtg
     2461 gtggatatat cggtattgaa ttagtagaag cttttgtcga atctggaaaa caagtgacat
     2521 tgattgatgg attagatcgc atcttgaata aatatctaga taaaccattc acagatatct
     2581 tagaaaaaga attgacagac cgcggtgtta ctcttgcgct aggcgaaaat gttactgaat
     2641 ttatcacaga tgaagaagga aaagtgaaac aagtagctac accaacagat acatttgata
     2701 cagatatggt cattttatgt gtgggcttcc gtccaaatac aaaactgatc gaaggaaaag
     2761 tagatacact gccaaacggg gcaatcaaag tgaatgaata catgcagaca agtaatccag
     2821 acatttttgc agctggagac tctgcagtag tcaactacaa tccaagtggc acgcaaaact
     2881 atattccttt ggcaacaaat gccgttcgtc aaggtttgct tgtgggaaat aacttgacag
     2941 atcataaaat ggcgtatcgt ggtactcaag gaacttcagg actttaccta tttggctgga
     3001 cgatcggttc gacaggtgtg accaaagaaa gcgcaacttt gaacgatcta gaagtccaag
     3061 cgactgtatt tgaagataat tatcgtccag agttcatgcc aactactgaa aaagtaatga
     3121 tggagctagt ctatgaaaaa ggaacgaatc gtatcgtagg tgctcagttc atgtccaaat
     3181 acgacattac acaatcagca aataccatgt ctttagctgt tcaaaataaa atgactgtag
     3241 aagatctcgc actttcagat ttcttcttcc aaccacattt tgaccgccca tggaattact
     3301 tgaacctact tgcacaagca gcgctaggcg aaatcgaaac tgcacaataa agaaaaaggg
     3361 ttgtgacaca attttgtcac gaccctctgt ctttttttgt gaaaagtaca attagttgtc
     3421 tttcttcgaa ttttttgtca caataagcag caaagataaa gctgttacat aaaagaacaa
     3481 gcagctttat ttgattttga aaagaaaggt gagaaagaag atgaataaag aatattttgt
     3541 ccaacaagat gcatttttag aagattataa aagtgcttcg aagaaattaa cgatcaatat
     3601 tgatgagttg gcagatcaag tactctctca gttgaatgaa acgcaagagg aatattacac
     3661 gatgccagca aatgacgcca aagataatgt ttctcatcgt tttcctttca gtagacgcat
     3721 ctactcagat gaaaatgacg gcacattgaa tacagagtac acatatgaag gtcagccata
     3781 tgtgctagaa actgatgatg atctttaaag acagtttttg aaaaggccgc tcatccttaa
     3841 gtgatgacaa aaaatcatca caattaccga tgactgtctc tttactatct tttaatcaga
     3901 aaaaggggtt atgacaag
//
LOCUS       DACCVU010000181         3827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCVU010000181 DACCVU010000000
VERSION     DACCVU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.186"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(457..990)
                     /locus_tag="IVW29_002833"
     CDS             complement(457..990)
                     /locus_tag="IVW29_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7840270.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1011..1955)
                     /locus_tag="IVW29_002834"
     CDS             complement(1011..1955)
                     /locus_tag="IVW29_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7840271.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2592..2678
                     /locus_tag="IVW29_002835"
     CDS             2592..2678
                     /locus_tag="IVW29_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7840272.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2799..3488)
                     /locus_tag="IVW29_002836"
     CDS             complement(2799..3488)
                     /locus_tag="IVW29_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840273.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3495..3773)
                     /locus_tag="IVW29_002837"
     CDS             complement(3495..3773)
                     /locus_tag="IVW29_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840274.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
BASE COUNT         1022 a          683 c          652 g         1470 t
ORIGIN      
        1 actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat ttttattggt
       61 ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc taattctttg
      121 tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt gttatgcttt
      181 ttacatatct catattcatt tattattctt ttctaaggtt aataatagga taatctcttt
      241 cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct acaaatactt
      301 cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt ttatttcctt
      361 tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg atagaaccga
      421 atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct gccaccattt
      481 tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag tcgttgaact
      541 attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt tttcgatttg
      601 ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta tatgagaatt
      661 attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac gcaactgatc
      721 tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat cgttgttttg
      781 ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag caagttgcaa
      841 tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc
      901 taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc
      961 ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat
     1021 aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt
     1081 tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt
     1141 tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt
     1201 tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta
     1261 aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt
     1321 ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct
     1381 tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata
     1441 tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc
     1501 tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa
     1561 gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt
     1621 atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac
     1681 atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca
     1741 taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa
     1801 tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac
     1861 acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt
     1921 aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc caattatgaa
     1981 tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa aatgtccttc
     2041 ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg
     2101 tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg taaagaggtt
     2161 taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg cctaaaaacc
     2221 acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc
     2281 ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg cgcagtcgcc
     2341 taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt ctgaatgctt
     2401 atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta aagcgcctta
     2461 caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa acaccacaca
     2521 agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc gactggaagg
     2581 aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt tgtgtctctt
     2641 tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac
     2701 ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat
     2761 catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg ttgctgatct
     2821 aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt tggattttgt
     2881 atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga ttgttgccgt
     2941 tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa attataacaa
     3001 gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg ctcatagttg
     3061 tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt gagtttttcc
     3121 atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat ttcctcataa
     3181 caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt tttatcacat
     3241 gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat acgtttattt
     3301 ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat aacccctggc
     3361 aataaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt ttgataagcc
     3421 ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata gggtaaatag
     3481 aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt catgattaag
     3541 caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt ctgttcttta
     3601 tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat tgtagtttgt
     3661 attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc attttctatg
     3721 tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt caacgtgttg
     3781 gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaa
//
LOCUS       DACCVU010000182         3780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCVU010000182 DACCVU010000000
VERSION     DACCVU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.187"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            190..564
                     /locus_tag="IVW29_002838"
     CDS             190..564
                     /locus_tag="IVW29_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840275.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            569..997
                     /locus_tag="IVW29_002839"
     CDS             569..997
                     /locus_tag="IVW29_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7840276.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            1047..1700
                     /locus_tag="IVW29_002840"
     CDS             1047..1700
                     /locus_tag="IVW29_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840277.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1836..2402
                     /locus_tag="IVW29_002841"
     CDS             1836..2402
                     /locus_tag="IVW29_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840278.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            2518..3654
                     /locus_tag="IVW29_002842"
     CDS             2518..3654
                     /locus_tag="IVW29_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840279.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         1449 a          601 c          690 g         1040 t
ORIGIN      
        1 gcctctaaaa taacatttct aagtttggcg accgagagaa tgttttaaat tagtagagtt
       61 ttttgttgct cttatttagc taacaattta gcttacgaat taaatatact agcttaaaag
      121 ctaattgtca accaaaaata taacttttaa gctatttatt ttcttttcag ctttgaaagg
      181 ttgataataa tgagtttagt tactaagatt aaagaattag cagacgaaaa gcatgtgact
      241 atagcagaag tagaaagaca ggtgggcatc tctaatggac aaataagaag atgggataaa
      301 gcctcgccaa aatctgagaa cttaaagaaa gttgctgatt attttggtgt cacaactgat
      361 tatttattgg gaaataataa tgttcccaag tgggctacaa aagaggaagt ggttgaactt
      421 gataaactac tagactcaaa tgttaatatg tcttatggtg gggaaacatt gacacccgaa
      481 caaatacagc gcgtaaaaga tatcctgata gcgactttct gggatattgt gaaagaagac
      541 aaagaaaaag gcaaaaagat gtgagcttat ggagatggat acgattaatt tagtcgagga
      601 gttgaagcgg aaataccagt ccgctaatcc tttttatatt tgtgaaaaga tgggcattaa
      661 aattcaatac gttcctttta tcgagaatcc caaggggcag tttcaagaaa ttagagatcg
      721 tgcgataatc tttttaaatg atgaactgcg agactctgag gaaagattct acatttgcgc
      781 tcacgaatta ggtcacgcta tttttcatcg tggcttatcc agctattatg tatcaacaag
      841 aacatctaga agcaaatctg aaagcgaagc taattgcttt gctgctaatc tcattgtttc
      901 tctttataaa gaagataatg atcaatatcc taaaaaaatc gaggaattaa agaatcttta
      961 cgggcttcca gaaagcgctt atcgttttct tatataaaaa agcccgtgtt agcacacata
     1021 ttacaacgag aaagaggaat tataaaatga aaaaagttag cgttatgttg ttgttaagta
     1081 ctgctctgct actttcagct tgttcaaata ataaaaaagc tgaatcaaca gataccactt
     1141 ctaaccaaga aacaaaaata agtaaaacaa aagaaacaac tgaaaccagt tcatctacta
     1201 gcaaatctac atctaaaaca gattctagtt caacagttac aagctccaac caagctacgg
     1261 cggaacctag cccaacagtt ataagctcca gtcagagtac aatccaaacc gcacctcaag
     1321 aagaaacata tgaacagatg aaacaacgca ctttacagtc aactccagct gatcgtgcaa
     1381 attggtccaa caaagagtgg gaagctttcg gcgtggccct ttatgaaaat ggattgacta
     1441 cagatgatgc tggcaatatt atcagtcaag atcagaaaga acaacaagca gcatctcaac
     1501 agaatccaga agaccaacaa acaagcgctc agcaagacgc tgacacttta tcacttactg
     1561 attttgttaa caaatacggg atgtcgcctg ttgcatggaa agtacagaat ggaatgtctg
     1621 aagaagaagc attgcgtaca acacagcaaa agacttccgg tgaagttcaa ttaggatttt
     1681 ctaaatacgg aattcaataa tacattttta tgccctacta ttttgcctat aatctctaaa
     1741 aaagttataa agaaaaaagc ccgtgctgca acacggactc atacctcatt tctgagatca
     1801 caaatatatt ataacaagaa gcgagggata tttaaatggc aaaaaaagtt atgggtcaag
     1861 atgggaaaat gtataaagtt aagaaaccgt tttataaacg ggtatggttt tggttgttag
     1921 cggttgttgt ggtgtttatt gctataggtt cgcaaggagg cagtgatgat gctaaaaata
     1981 ccgtcgctga aacaactaaa gaaagcgtga cagaagtgtc ttctgcagaa tcggtagcag
     2041 aatctacagt cgttgaagaa gaaactgaaa ctactgaaac tactatagaa gaagttactc
     2101 aagaagaaag tgttcctcgt gaatacagaa atgcattgag caaagctgaa agctatctag
     2161 gttgggctgg tatgtctgaa caaggtttgc gtgaacaact agagtttgaa gaatatccaa
     2221 gtgatgcaat cgattatgcg ctggctaatg ttgatgtcga ttacaacgaa caagctttgg
     2281 ctaaagcgga aagttacgat gattgggcat caatgtcaga ttcgcaattg tacgatcaac
     2341 ttatatttga aggttttaca aatgagcaag cacaatacgc tttagataac ctaccacaat
     2401 aactaacaaa aacacgcccc tccctcgcca aagtttgtgg acgtgatgaa aaataaacct
     2461 gctataatcg gcttacttat ctattcctat tatagcaaca aataggagat gaaaacaatg
     2521 tggatcgaaa ctaaaactga taaaaacgga aaaaaagtat ataaatataa tgagcgatat
     2581 attgatccaa aaactagaaa aagaaaaaaa gtgagcatta cttataaaaa taaatctcga
     2641 gaaactcaga aagtggcatt gctagagtta aataaaaaaa ttgatataaa attaaacgaa
     2701 aaaacacttc ataagcctga tctaacattt catgagcttg ttgaagaatg gttagttata
     2761 tacaaaagac aagttaagga atctacatat tatcctacga ataatatatt aaacactata
     2821 aagaagaaga tacctgagac ttacatcgtt tctggtatta atacaataga tttgaataat
     2881 atttttgaag acatgatata taaagatgac ttgtcaaata agtacgtcag tgtaattaaa
     2941 tccaaattga atcttctttt ttcatatgct atgaaaaaag gttatgtaga aaaaaatcct
     3001 atcgatgaag tagttattga ttataaaaga gagtcaaaaa cgataaaaat taaagataaa
     3061 tttttagaag atgatgaata taatagacta gtagatttca caacttcaca caataaaaga
     3121 tattctctcc ttttccagtg gctatatttg actgggatga ggtctggtga agcaatcgca
     3181 ttaagtaaag atgatgtaca cattaccaat aataatgcat cagtagttat aaatgggacg
     3241 atgatgtata gagaacgttc aatagctgat atgaaaaaat ctgattctac aaaaacagct
     3301 gccggaatgc gagaaattga tttaccaaaa aaagcgatag ctatttacaa tgagcttcta
     3361 gaattaaatc caaatggtca atttctattt caaacaacga aaggaactcc tttccaacta
     3421 acagcaatta acacctattt gagaagtcat aaagctgaca tgaagattga taaaaaactt
     3481 agttcacata tttttagaca tacccatatt tcaaaattag cggaactagg aactcctctg
     3541 tatgccattc aggatcgcgt tggtcacgaa aatagtgata tcactgaaaa aatttatttg
     3601 catgtgacaa aaggagtaaa agaaaaattg aaagaagata tagaaaaact gtaacttgtt
     3661 gccccttttt tgcccctttt tattaatcaa aaatgtacca aatagaaatc gttaatataa
     3721 aaaaacagat aataaaaaaa gaacccttga ataccaaggg ttctttctct gtctgataac
//
LOCUS       DACCVU010000183         3755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCVU010000183 DACCVU010000000
VERSION     DACCVU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.188"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            14..1141
                     /locus_tag="IVW29_002843"
     CDS             14..1141
                     /locus_tag="IVW29_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840280.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IVW29_002844"
     CDS             complement(1359..2501)
                     /locus_tag="IVW29_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840281.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="IVW29_002845"
     CDS             complement(2506..2691)
                     /locus_tag="IVW29_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7840282.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="IVW29_002846"
     CDS             complement(3045..3290)
                     /locus_tag="IVW29_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840283.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="IVW29_002847"
     CDS             complement(3284..3685)
                     /locus_tag="IVW29_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7840284.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1227 a          681 c          575 g         1272 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaact
//
LOCUS       DACCVU010000184         3722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCVU010000184 DACCVU010000000
VERSION     DACCVU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.189"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            24..788
                     /locus_tag="IVW29_002848"
     CDS             24..788
                     /locus_tag="IVW29_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7840285.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            1277..3370
                     /locus_tag="IVW29_002849"
     CDS             1277..3370
                     /locus_tag="IVW29_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7840286.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            3395..3697
                     /locus_tag="IVW29_002850"
     CDS             3395..3697
                     /locus_tag="IVW29_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7840287.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         1274 a          625 c          780 g         1043 t
ORIGIN      
        1 aataaaagat aacgaggaag acaatgaaaa aaatcaatta tttagcaatc attttgttag
       61 tgggcctatg tttgtttgct ctgccgaccc aaggattcgc tgtcgcgggt ggtcatggtg
      121 gaggtacatc tagcggtcga agttcgggca gaggttcatt tggtggtcgt tcatatgggg
      181 gcagttattt tggcagtcgt tcttacggca gagattctta tgggtattat ggtccaggta
      241 caggtccttc tagttatgag caaacgggat ttcttgcatt agctgttttg tcagtaggag
      301 gggtttctgc tttttctatc gtcaagaata aaagacaaaa gaaaaggctg aaagcggaga
      361 tattgtcccg catgtcagga acggctaaag aaaagaaagc acagtttctg gaaatccaaa
      421 agatattcat gaagatccaa tccgcttggg aagaagaaac tcctgaaaaa gccagaagct
      481 gttatacaga caagctgttt gccgaacatc agaaagttct gcaaaaaaac aaagcagacg
      541 gtgtaagaaa tcatactaaa aaaataacta ttcaggaagt aggaaattat cgacagatcc
      601 acgaaaatag ttttgccatc cgaattgatt tcagatgctg tgattattca gtcgatcgaa
      661 aagatgggcg attgatctca ggaagtaaga cacaacgaca gtatttttct cagatatggt
      721 attttgattt ttctgagaaa gaaaaaagat ggcaagccga ttttattcag ccgattatgc
      781 tggattgaca ggaaatattt atgaacggag ttgtgacaaa agccttgtcg cagctccttt
      841 ttctgaataa acggtgtgcc agagatagcc tatcggcaat aaggcacgat tttgtaaaat
      901 atgggaagcg atttttcaaa atcgcttctt atcgctcaag gctgaacatc tctgtcacaa
      961 cctctccact aaatggtgtt taaaagcttg catctgcgat aataagaaca accaaacgaa
     1021 aaataggaag cactatttta gtttagttgt tcttattact tgatgcagga cagctttttc
     1081 acaacctctt ttttgtatag atgggcaaaa tttttgtaat aataggcgta tgtctttttc
     1141 gttgaggaga tgaaaaagca tatggcttat tttttgtttg ctttgcttac tttctttcca
     1201 cttcaagtgg cagaaatatc tactttttat tatgtaagcg gaaccatata cttgaaatca
     1261 gaaggagatc atttctatga aaacaagaac aattgaaata cttcacagaa tcatgacagc
     1321 ccaatatcca gtaaaagcaa aacatctagc aagtgagttc aatgtcagtc agcggacgat
     1381 ccgtcaggaa gttcttgaag cgaatatctg gttgagcagt aaaaagcttc cggagatccg
     1441 tacaattcgc aacaaaggtt ttttgttgaa gctggaaaag gaaaagcaag ctcgattaga
     1501 aactgaaata aaagatgtga aagacggctt tttgaatcgg cgcgaacgta tttttgatct
     1561 agttttagcc gttgcttaca gccgaatccc gatccatcta aacaaaaaag aagaagaatt
     1621 tcaaatctct aaaagtacat tagatgaaga tatgagacgg ttacgagcaa cactggtcag
     1681 ttatgggata gagatcgtca gttatggaaa gcaaggactg gtttataaag gttcagaacg
     1741 atctatccgt acgatgatct atgattttat caatcagaat ctgggaatga ccgacttctt
     1801 tatgaatcaa gatgcacaat taacaccggc tcaaaaaatc tttctccatt attttccttt
     1861 ggtggaaatc aaaaaaatac aggagatcta ttccaataag ttgttgaaac aagaagatga
     1921 tatttataaa aaacaaatcc tgctttttac attgatctgg atccagcgaa tcaggaaaca
     1981 tgaatttatc tctgccatta attggaaaaa tacagaaata aaagacagta gcttttctgc
     2041 atttattgac cacatgatcg aggaatttca attggacaat gtcccgcaag tagaaagaaa
     2101 ttatcttcgt ttcactattg agacgttcaa tgctagagat atctccaatt caatcgagtg
     2161 ggtacaagct caattactaa cgatccaatt gatccaattt gtagagaacg agacacacgt
     2221 acctttccat ctaaaggaag aagctttatt cgaaaatctc tataagcaca tggctgcact
     2281 gattgtaaga atcaagaatc atatccaagt catgaatcca ttgaaagaaa acatccgaac
     2341 aaattatttc ccaatctacc gcgcagtcac tcattttgtt cctacgatcg aagaagtaat
     2401 tggtggcaag atactggagg acgagatagc ttttcttgtc atccactttt cgacgatcgc
     2461 cagtgtaatc aagcgagact tgaattatgt ttataaatca gtcgttgtct gtaaccatgg
     2521 gatggctact ggaaatctgt tagcagagaa tctgaaagaa aagtttcctc agatcgagat
     2581 tactgcagtt ttaagttcaa aagaaatcga attagtagat aaattggatg tcaatctggt
     2641 tttcagcacc tttcaattga attatcgaaa taagccgctt ttagttgtcg atccgatttt
     2701 aactgagacg aatatcttgg ttatagaaga ctttttgaaa agaaatgaag aactacagcg
     2761 gttagagcct aacagtcatg aatcgacgga gttattcact caaataatag atgtcatgaa
     2821 agaaagtggt ggccgagtag atcgaatcat ctatggcaag ttagaaaaat tattcgaaac
     2881 aaacaattta gaaatcaaca aaagggagat ccagcctatg ttaaaagata ttttgacgga
     2941 cagcaatatc atgatcaaag aaaaagccac tacttgggag gaatcgattg aactagctgc
     3001 tagaccattg gttaaagaaa acattgtcga agagcgctat gtcgaagcga tgattcgtgc
     3061 agtgaatgat tacggaccgt atatcgtgat tggcaaacac atggcactag cccatgcccg
     3121 accagaagat ggggtcaata aattaggagt cagcgtagct acgattgagc aaccaatcga
     3181 tttcggtaat cctgaaatgg atccagtaaa gattattttt tgtttggcag cagtagactc
     3241 ctactcgcat ctgaacatca tgaaagaact aattgaattg atcaacaacg aagagaagtt
     3301 ggatcgattg attgaatgca caaatatcga agatttcaaa caactgcttt tttcagtagt
     3361 tgagaaataa taaatgatac gggggagaac aaagatgaac aaattgaaca ttttattcgt
     3421 atgcggtgca ggtttaggaa gtagttttgc agcacagatg tctgctgagg atgttttgaa
     3481 caaattagga gtagaagcca aactggacca ttgtgatatt tcttccgcag tttccatgaa
     3541 accagatgtg atcatcacag cagaaaattt tcgttcgcag ttcgaaaagt tcgcagttga
     3601 tcctaaaacg gcgatgatct atttgaaaaa tattgtttca aaaaatgaaa ttgaagaaaa
     3661 aatcacccca gttctgcaag ataagggagt tatctaaata caaaaagaaa agagggtaaa
     3721 aa
//
LOCUS       DACCVU010000185         3696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCVU010000185 DACCVU010000000
VERSION     DACCVU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.190"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            152..496
                     /locus_tag="IVW29_002851"
     CDS             152..496
                     /locus_tag="IVW29_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7840288.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            497..916
                     /locus_tag="IVW29_002852"
     CDS             497..916
                     /locus_tag="IVW29_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7840289.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1008..1358)
                     /locus_tag="IVW29_002853"
     CDS             complement(1008..1358)
                     /locus_tag="IVW29_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840290.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1324..2655)
                     /locus_tag="IVW29_002854"
     CDS             complement(1324..2655)
                     /locus_tag="IVW29_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7840291.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2911..3477
                     /locus_tag="IVW29_002855"
     CDS             2911..3477
                     /locus_tag="IVW29_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7840292.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1366 a          630 c          719 g          981 t
ORIGIN      
        1 tatgcttaaa aagcatatcg actgaataaa ctaagcattg gacaggctga aagactacat
       61 ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc cttagaacca tagttgaatc
      121 acctaacagt cgtgaaggga gaataagaga aatggcagaa aaacaaaccg caggacgaga
      181 tcaattagga gattttgcac caaaatttgc agaattgaat gatgatgttt tatttggaca
      241 agtatggtcc agagaagaat tatctgctca tcagcgtagt atcatcacca tatctgcatt
      301 aatttcagga ggaaattttg aacaattgcc agctcatctg aagattggca agcaaaatgg
      361 cattactagt gaagaaatag cagaaatcat tacccattta gctttttatg taggatggcc
      421 aaaagcttgg tcagctttta gtttagcaaa agaaatctat ttagaagaaa atagcgaaga
      481 taagaaagag gcgtaaatga cgacaaaaca cgaagaaata cacaatggcg tgatttttcc
      541 agcaggtgaa aaaaacgaag cttacgctca agtatttacc gggcaaagct atctgaatat
      601 gttagtgaat gatcccgaag tttctgttgg tgtaggtaat gtaatttttg aaccaggttg
      661 tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc ttacttgtga ccggaggaga
      721 aggctggtat caggaagaag gaaagccagc aagacgatta atggctggag atgtcgtcgt
      781 aacgcacaaa gggattaaac attggcatgg tgcgacaaaa gacagttggt ttgaacattt
      841 ggcgatcact gctggtaatt cagagttttt agaacctgta acagatgaag tgtatgaagg
      901 attggaaaat caataatgtc aagcttgaac agctcctgaa agtagatttt aatcgaactt
      961 tcaggagctg cttttttgat tcagtctttt ataaatagaa aatagggtta ataataggaa
     1021 tgagaagcac gtcgaaagat cttaatattg ataatcggct gggcatacat gatttccacg
     1081 tcatcaatca aactacctaa cagatgataa tcttcccgat catgtcgggg attacctaat
     1141 agttcttgat agtagatttc catactaggg tcccgaggat tgttcagcaa atcagcaagt
     1201 acatctaaat cttctggctg agtttctgcc gtaccctgta tctctatgaa aagcccttcc
     1261 tcagcaaatt ctactccaat acgtatttca gttatttcat gggaatataa ataagtaaga
     1321 agctcatcga tgattctttt tgataattct ttttccaagt gtcttttttt cctttctgac
     1381 agcggtaaag ttatctagta tcggtacaag gatacctgca acgaaaccag ttgaaaaacc
     1441 actgttatat agatttagtc ctccgtgcaa agtgctgacg ttatggacaa gagcaatatg
     1501 tagtacacca gctattacac cgtaaaaact gccgtaaaaa ccgcttatgg gcgcgagacc
     1561 tgtcccaaac acagctgcaa caagcatgcc agtactggtg atctcatgaa aagttccaaa
     1621 caaagaagca ataaatattc cgactagtat cggaaaactg ttttttaaat gacaaccaaa
     1681 agcactgaaa cccaccgcag ataaaatagc accgatgaca ggtccattta actgaccgcc
     1741 aaccagcaaa acataagaag acagcatcag accaactaag cctatgttga tcaaagtagc
     1801 accaatacca gaattagcaa tgaaatcagt tgtcaatttt ccagatgaat cgaaaatttc
     1861 tcgaatcccc aaaagccgaa aggaattata atagaaacca attccgactg tcagcgaaaa
     1921 aaagccaaaa ataatccaaa tgagaggaaa gtgatattca ttgaaaacca atgtattttc
     1981 gacgattgaa tagctgaata acctcaaaac agcagtaaaa gtcatcgcaa tcaacccaga
     2041 ggtgaatcca atattataaa gtgtgaatcc acgatggaaa actaaagttt gagcagctaa
     2101 gggcggaaga agtagaccaa ttgctatgcc agctaaattc cccaataaaa tacctatcag
     2161 taaaggaaaa cgcataccaa aagtgatata actaacgacc ggtgataatg cgctaccaaa
     2221 cagtccgatc atgatgtact gagaaaaagg tttattgaca aatacagcgt atagatacac
     2281 acctaaaatg attgagatag aattataaag gtttttgcca aaaaaagaga aaccggtaac
     2341 tgttagaatc gaagcaaata agggaccgtt taaaatcacc ttgtgccgat aagctaaaag
     2401 cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg cttccgaaac cgcctaaagc
     2461 aaaataatct gtcaaaagat tgcttggcga ggtaagaata gttagcaatc cactccaatt
     2521 atcagtagga gtattagaaa gcaagccaat aaaaagaaaa aaaacacctg taaaaccaag
     2581 ataaaaatac tttcttttct gtgaaacgat tcgtttttga ggatgaatat aaataacgtc
     2641 agtttcgttt tccattacac accttctttt tcattttctt taactataac ttgaaatgaa
     2701 agatttggac aactatcatt taggtcaaaa ctaaaaatgt aagggaaccc attgaattta
     2761 accaaatttt tttaattttg caaaaaaaag ttaaagaaga ctgaaaaaaa atttaaaata
     2821 taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat agggtacaat aaaacaacat
     2881 atactgcagt aaagaagtgg gggagtttta atgagaagaa aagtctatac caaggatcaa
     2941 attctaaaag ctgcttatga tgttgttgca aaggaagggt ttaaaggatt tactgcgaga
     3001 aatattgcta aaaagatggg gatttcgaca cagccgattt atctggaatt caaaaacatg
     3061 gaagatttaa aaaatacgtt gctggattct atctttgaac aactaaaaga agatgttttc
     3121 cctattgaac gtactggtga taaaatcatt gatctcggtt tgaactatat ctactttgca
     3181 aaaaagcatc atagtcttta catggcattg tacattgatg aacatgggga cggaaagaaa
     3241 atgcatgatt tttcatacaa ttatttccgt gaaatagtta aagaagatga aagatacaat
     3301 caattatcgg atgaatatgt cgatgctctg catactggta catgggtagc agtgactggc
     3361 ttagcgtcat tgatgtcatc aaacatcatg catccaacag aagaacaaat cattgcactg
     3421 atcaaacgga caattgactc gattttagca ttagataatc ctgaaataat cagatagtgt
     3481 tctatcagta tgaatttgat cggtaagtcc gttggctgca aactgtcaat ggactttttt
     3541 gctaaaaaaa gcgatttctg ttatttatct attagaaata ggcttgacat ttcgtgaaaa
     3601 aagtttaaaa atagtactta cggaaggttg gcagagtggt aatgcaccgg actcgaaatc
     3661 cggcgaaccg gctaataccg gcgcgcaggt tcaaat
//
LOCUS       DACCVU010000186         3663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCVU010000186 DACCVU010000000
VERSION     DACCVU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.191"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..276
                     /locus_tag="IVW29_002856"
     CDS             <1..276
                     /locus_tag="IVW29_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP7840293.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IVW29_002857"
     CDS             419..640
                     /locus_tag="IVW29_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840294.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IVW29_002858"
     CDS             656..1498
                     /locus_tag="IVW29_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840295.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IVW29_002859"
     CDS             1561..3363
                     /locus_tag="IVW29_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP7840296.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="IVW29_002860"
     CDS             complement(3422..>3663)
                     /locus_tag="IVW29_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7840297.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DACCVU010000187         3633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCVU010000187 DACCVU010000000
VERSION     DACCVU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.192"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..572
                     /locus_tag="IVW29_002861"
     CDS             <1..572
                     /locus_tag="IVW29_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7840298.1"
                     /translation="YHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPII
                     CILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAA
                     SFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDG
                     NSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            545..1222
                     /locus_tag="IVW29_002862"
     CDS             545..1222
                     /locus_tag="IVW29_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7840299.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1212..2354
                     /locus_tag="IVW29_002863"
     CDS             1212..2354
                     /locus_tag="IVW29_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7840300.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2367..3344
                     /locus_tag="IVW29_002864"
     CDS             2367..3344
                     /locus_tag="IVW29_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7840301.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3344..>3633
                     /locus_tag="IVW29_002865"
     CDS             3344..>3633
                     /locus_tag="IVW29_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7840302.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSES"
BASE COUNT         1371 a          453 c          680 g         1129 t
ORIGIN      
        1 aatatcatct aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa
       61 ccgaactaga aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat
      121 ctcaaccaat tatttgcatt ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa
      181 tgtaccagaa atatttaaaa acggatatta tttatattac gaatttagag ccggaggaat
      241 acctgactat attaaaagag tgcaaattta tgatcggaaa ttcttcttct gggataattg
      301 aagctgcatc attcaaaata ccagtaataa atttaggaac cagacaaaat ggacgagaac
      361 aatctaaaaa tattataaat gcaaatctaa atgaaaatga aattgaaact gctttgaata
      421 aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt taagaacatc tacggtgacg
      481 gcaacagttc tagaagaata acaaaaacat tgaatcaaat attgaatgat ggggaagata
      541 ttaagtggat tcaaaaacaa atagcttatt aaaatatact agtgatccat acattattat
      601 tggggctggc ggacacgcaa aagtcattat tgaaacctta agattagaaa ataagttaat
      661 atatggattt tttgatgata agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat
      721 aaacgaattg aatagctggg cggatagcaa aaaaaaaatc tataaattta ttgtagctat
      781 tggtgactgt gtagtaagaa aaaagattat aaaaaaattg aatatgccaa gtgagcaata
      841 tggaatagct gttcatccaa gtgctatagt ggcaaaagaa atttcaattg gttatggaac
      901 tgttattttt gcgaatgctg taattaaccc agatgctgta attggagagc atgctataat
      961 aaacacaggt agcattgttg agcatgattg tagaattaat aactatgttc atttatctcc
     1021 aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga actcaaatag gaattggttc
     1081 tcaatgtatc cagatgaaga aaattggatc ttggtgtatg ataggcgcag ggagcactat
     1141 tgttaaggat attccctcac attccttagc ttatggcaat ccagctaaaa ttaagaaaga
     1201 aggaattaat tttgaattct agaattttat tatcaagtcc tcatatggga ggaacagaag
     1261 aaaaatatgt tgctaaggct tttaaagata attggatagc gccattaggt gaaaatgtgg
     1321 ataaatttga agaaagtatt acaaaatata ctggttctaa gggcggagct tctgcgttaa
     1381 gttcaggtac agctgccata catcttgcct taagactgct taatgttgga aaaaacgata
     1441 ttgtctttgc tccttcattt acttttatag ctacagtaaa tcctatttta tatcaagggg
     1501 ctgtaccagt gttcattgac tcagaaccta atacatggaa tatgagccct gttgcactag
     1561 agagcgcatt agaagaagct gaaagaaaca accaattacc taaggcaatc atcgtagtga
     1621 atttatatgg tcaaatggct gattatgaac gaattttagc tattgcaaac caatttaatg
     1681 ttccagtgat agaagatgca gcggaatcgt taggttctac ttataaggga atttcgtcag
     1741 gcttatttgg gacaattggg atctattcat ttaacggtaa taagatcatc acaacctcag
     1801 gtggaggagc tctagtgtct agtgataaaa aatatgttga gcattcaaga tttttagcaa
     1861 cacaagcaag agatcaagca ccgtattatc aacatagcga aataggttat aattaccgtc
     1921 taagcaatat ttctgccggt atcggacgtg gacaaatgga agtattgaat gatagagtag
     1981 ctaaacgaag agaaattttc aatcgatata aggaagcttt ttctggaatt gatagaattg
     2041 aaatgatgcc agaagtggat ggcatggtgc caaatcgttg gttgtctact ttaacgatca
     2101 atgattataa ttctattgat caagtagtta acgtaataaa taaactaaat gaaaaaaaca
     2161 ttgaaacaag agctttgtgg aaaccactac atttgcaacc agtatttcaa ggggtaaaaa
     2221 tgtattacca tgataataat cttagtgtgt cagaaaagtt atttaatagc ggcttatgct
     2281 taccttctgg aagtaatatg acagatagtg aacaagaatt tgttattgat gagtttaaaa
     2341 aaatattcaa gtaggtgtga tattttatga acataggaat tattggttta ggttccatgg
     2401 ggaaaagaag acttagactc attcaagaac actttaatga tattttacta tgtggtgttg
     2461 atactaatga ggcgagacga aaggaagttg aaaaggaatt tggaatacca acttataata
     2521 gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt ttttgtttgt acatccccta
     2581 taacccatga atttattata aaagaggctt taaatttaga ttgtaatatc tttacggaaa
     2641 tcaatttatt aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat
     2701 tactttatct atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag
     2761 tgacgcataa taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc
     2821 atccttggga agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag
     2881 aaatattcgc tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta
     2941 ctgtaatgac ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta
     3001 ttattgagca tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag
     3061 ctaaaagaaa tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg
     3121 attctctatt ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg
     3181 aggttatgca tgataataat tattcacata ctattattga agatgcatat ttagaggaga
     3241 ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc ccaatatact tttgaaaaag
     3301 ataagtatat tattaactta attaacgaca ttgaggtaaa ttaatgatga aagtaatttt
     3361 ttttgggtta gggtcaattg gaaagagaca tctaaaaaat ctaataagct atggaaaaaa
     3421 gaattctatt aattttgata taacagctta tagtagcagt ggaaaaaaag agtttgaaga
     3481 aataacgtat attcaaaata gagacgattt ggatacacat tatgatatag cttttattac
     3541 aaatccgact tttaagcatc tggaaacact aaatgtaatt gttgaaaaag ttgattacat
     3601 atttttagaa aaacctgctt tttctgagag tat
//
LOCUS       DACCVU010000188         3585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCVU010000188 DACCVU010000000
VERSION     DACCVU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.193"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            166..1932
                     /locus_tag="IVW29_002866"
     CDS             166..1932
                     /locus_tag="IVW29_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7840303.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1947..2384
                     /locus_tag="IVW29_002867"
     CDS             1947..2384
                     /locus_tag="IVW29_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7840304.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2397..3548
                     /locus_tag="IVW29_002868"
     CDS             2397..3548
                     /locus_tag="IVW29_002868"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7840305.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1203 a          636 c          772 g          974 t
ORIGIN      
        1 aaaataacag ttttatttaa aattgaagtt ttctgatacc gaaatctttt tattttgctg
       61 acatactcaa acctttggca caaagtttcg tgccaaagta cgactatttc tctttttagt
      121 aagcggatac aattagagta tcaaaaggag agagggagag acaaaatgga acggacgatt
      181 cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat taggcagtac tttatcaagc
      241 atggtcatgc caaatattgg agcactgatt gcctggggtg tattaacggc tctatttatc
      301 ccagatggtt atttaccaaa tgaggctttg gctaccatgg taagcccaat gctaacttat
      361 ctgattcctt tattgatcgg ttatactggc ggaaaagtaa ttgctggtga ccgcggatca
      421 gttgttgggg caattgcaac gatgggtgtg attgtcggaa cagatattcc aatgatgcta
      481 ggcgcgatga tcatgggacc tcttggtgga tatgccatca agaaatttga tcagctattc
      541 caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata atttttcagc aggtcttatc
      601 ggttttggat tagcgttatt aggtttttct gcgattggtc cagttgtcga tgcattgact
      661 caagcaatgg caaaaggtgt agagatcatt ttatctgccc atttgattcc attgacaagt
      721 atttttattg aaccagcaaa aatcttattt ttgaataatg caatcaatca tgggattttg
      781 acaccattgg gaactgaaca agtagcagag gctggaaaat ctattctatt cttgcttgaa
      841 gcaaatcctg gtccaggact tggcgtatta cttgctttta ctttgtttgg caaaggagca
      901 gcaaaatctt cagcaccagg agccatgatc attcactttt taggcgggat ccatgaaatc
      961 tattttcctt acgttatgat gaaaccgtta ttattcttat cagttatagg cggaggaatg
     1021 tcaggaagtt tcattttcca attgatggat gctggactaa gagcgccagc ttctccaggg
     1081 tcgatcattg cgattctagc gatgacaccg ttcaattctt accttcctgt tattttgggc
     1141 gtgattgttg ccgcagctgt ttcgtttggt atttctgcag taattttgaa agcggatgcg
     1201 aaagatacaa atgaagactt cgaaaaatct gttcaagaga caaaggcagc taagcaagct
     1261 tccaaaggtg cccaaggtat tcctcaagga gctacattct ctggcgtaca aaaaatcatt
     1321 tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa
     1381 gtacaggaag cagacttgcc tcaaacggtg accaatcaag caatcagcca actgacagat
     1441 gactcgaata cattgatcgt tactcaggtc gaactacaag aaagagcaaa acaaaaagca
     1501 ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt cacctcgata tgatgaaatc
     1561 attcaagaat taagcagcaa gcagaaaaca tcagtttctg aaattgagaa tgagccagca
     1621 gaagagtttc tttcagcaaa catcaaacaa atcaaagaga ttctacttgt ccacgatgat
     1681 cgttccggat cagcaacaat ggggatgacc gtgctcaaag atatattaga caagaaccat
     1741 ttaactatcc cgttaagaaa agtcaatatc caagatttaa aagatgaacc tgattcactg
     1801 atcgtagcca aagaaagttt agcggaaaaa gtacagatgg ctgcacctca atccattcat
     1861 ttatcagtca acagcatggt caatccgcaa aaatacgaat cgattgcaca atatttgaaa
     1921 cagacagctt aagggaggaa aacagcatgc aaagtttgcc attcgacatg atcgagctca
     1981 atcgatcttt taaaaccaaa gaagaagcga tccgctattg cggtcagatg cttgtagaag
     2041 cagggtgcgt tgatgaggaa tatatcgatg ccatggtaga tagagacaaa atgttatctg
     2101 tttatatggg gaattttatc gcgattcctc atggaacaga tgaagcaaaa aaatatgtga
     2161 aaaaatccgg catctgtgtc gttcaagtac cagatggtgt agattttggt accgaagaag
     2221 aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt cggagatgag catttgcaac
     2281 ttgtacagca aatcgcactt tattgcagtg atatggacaa tgttgtccag ctagcagacg
     2341 cgctcagcaa agaagaagta acggaaaatc tagcaattgc ttaaggagag agaaaaatga
     2401 gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt tattggagag gttttggcag
     2461 ctaatggatt tgagattact ttcgtagatg tgaatcacac gatcattgat gctttaaatg
     2521 aaagaaaaga atacgaaatc gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa
     2581 atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt aatccaagaa atcgctgaag
     2641 cagatttagt tactaccgcc attggcccga atatcttacc ttttattgct gagttgattg
     2701 caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac atctttagat atcattgcct
     2761 gtgaaaatat gattggcggt tcagcatttc tagaaaaaga agtgtataaa tatcttccgg
     2821 aaacatcatt tactgacaaa tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc
     2881 cattacaaca ccatgaagat cctttattcg tccaagtgga acctttcaaa gaatgggtca
     2941 ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa aggggtttta tatgtagatg
     3001 atctagaacc atatatcgaa cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg
     3061 cttatacagg tgccttgtta ggttaccaga ccattgatga agcaatgcaa gatgcgttag
     3121 ttgtggcaca attaaaatca gttttgcagg aaacaggaag tttgctgatt gccaaatggg
     3181 ggtttgatgc agagcagcat catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca
     3241 aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc attacgtaag ttaggttatc
     3301 aagaaagatt tacacgtcca gtgagagaac tacaagaaca taacttgact tgcccacatt
     3361 tgacggcaac aatggggatt atttttaatt actatgatcc tgaagatgaa caaagccgtc
     3421 aattgcatga aatgaagatc catgaaaact tggaacaatt gattcaagaa gtcacaggaa
     3481 tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt gaaccgttat gccaagcaag
     3541 tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa ttttt
//
LOCUS       DACCVU010000189         3583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCVU010000189 DACCVU010000000
VERSION     DACCVU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.194"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVW29_002869"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVW29_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7840306.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="IVW29_002870"
     CDS             complement(3004..3438)
                     /locus_tag="IVW29_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840307.1"
                     /translation="MRLYHLRSLGELMELDFDKNFFWEIPDLSCFVESERLSNGNNSA
                     LTEHDRVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..>3583)
                     /locus_tag="IVW29_002871"
     CDS             complement(3455..>3583)
                     /locus_tag="IVW29_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7840308.1"
                     /translation="SEVSLGLGFSDSSHFSRVFKKYVGVSPKQYQLGLVDSFSTSN"
BASE COUNT         1071 a          706 c          510 g         1296 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactc tgtcatgttc
     3301 tgttaacgca ctattattcc cattacttaa acgttcagat tctacaaaac aactcaagtc
     3361 aggaatctcc caaaaaaaat ttttgtcaaa atccaattcc atcaactccc ccaaactacg
     3421 taaatgatat agtctcacaa taaaataaag tctctcagtt actagtgcta aagctatcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctacgta cttcttaaat actcttgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc act
//
LOCUS       DACCVU010000190         3561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCVU010000190 DACCVU010000000
VERSION     DACCVU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.195"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2303)
                     /locus_tag="IVW29_002872"
     CDS             complement(<1..2303)
                     /locus_tag="IVW29_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840309.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IVW29_002873"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IVW29_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          997 a          791 c          561 g         1212 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa c
//
LOCUS       DACCVU010000191         3530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCVU010000191 DACCVU010000000
VERSION     DACCVU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.196"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            140..1021
                     /locus_tag="IVW29_002874"
     CDS             140..1021
                     /locus_tag="IVW29_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7840310.1"
                     /translation="MTEIVKVQGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKS
                     TTIRTLLGIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFI
                     KLHGGGSKAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSG
                     LDPLMEAVFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELR
                     HLTRSTVTLVTEGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVTKI
                     ESVPPTLEDLFMRHYEG"
     gene            1045..2658
                     /locus_tag="IVW29_002875"
     CDS             1045..2658
                     /locus_tag="IVW29_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7840311.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(2730..3242)
                     /locus_tag="IVW29_002876"
                     /pseudo
     CDS             complement(2730..3242)
                     /locus_tag="IVW29_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3241..3530
                     /locus_tag="IVW29_002877"
                     /pseudo
     CDS             3241..3530
                     /locus_tag="IVW29_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
BASE COUNT         1073 a          600 c          778 g         1079 t
ORIGIN      
        1 agaactttgg tttgaaacaa agtataaagg ggtattttat gaacgataca tattgtatta
       61 gttcataaaa aaagtgcgtt ttgttcagac tggaaagctc gttataaaat catgtcaaaa
      121 gaaaagggag ttatgtacca tgacagaaat tgtaaaagta caaggcttgc aaaaaaaatt
      181 tggtaaattc caggcgttga aagatgtctc attcacagta aacgccggtg aagttgttgg
      241 ttttatcgga ccaaatggag caggaaagtc aacgacgatc cgtacactgc taggaatcat
      301 caacagagac gaaggagatg tccaaatatt cggaaaagat gtttggaaag atagtctaga
      361 aatccataaa cgaatttcgt atgttcctgg agatgttgct ctttggggca gcctgactgg
      421 tggagagatc attgatctat ttatcaaact tcatggcggc gggagcaaag caaagcgcga
      481 ttatttaatc aaacgatttg aacttgatcc aaagaaaaaa gccaaaggtt actctaaagg
      541 aaatcgtcaa aaagtcggtt tgattgctgc actttcagtt gaatctgatc tgtatatttt
      601 agatgaaccg acttcaggac tagatccatt gatggaagca gtattccaag aagaagtaga
      661 aaaaatcaaa aatgatggca aagcgattct attatcttca catattttaa gtgaagttga
      721 acgattagca gataaagtag caatcattcg acgtggagaa gtagttgaaa caggtacatt
      781 agatgaattg cgtcatttga ctcgctcaac agttacattg gtgacagaag gcgatattga
      841 gaaacttgcg acgctctctg gcgtgcatga ttttgttcaa aaagacggca aagcaacttt
      901 ttctgctgac aatgaagcga tgaatacgat tctgaccgag gcaaccaaat taggtgtgac
      961 aaaaatcgaa tctgtaccgc caacgcttga agatctattc atgcgtcatt acgaaggctg
     1021 attgtcggaa cggaggaaaa gaaaatgaat gaaaaatttg cccgttggaa cgtattgttc
     1081 attcaatacg tgaaacgcga ttggaaaaaa ataattgttt gggttttagg tttgggtttg
     1141 ttctcaggag catttgtacc agcatttgaa gagattgcta aaggacaagg tcttttaggg
     1201 atgtttgaaa cgatgcaaaa tccagcgatg atctcgatgg ttggacctac accaatcaaa
     1261 ataggtacgg attatacttt aggagcgatg tatgctcaag agatgttgct gttttgcgga
     1321 ttgttcgcaa tgattatctc agcacttcat gtggtgagcc acacgcgaaa agaagaagaa
     1381 ttaggtttga ctgaattggt tcgctcattt cgagtaggac gacaagccaa ttcattagct
     1441 gttatcagtg agatgctgtt gatcaatctt ttattaggtc ttttaatcgg cggactcatg
     1501 atgagttttg gtgtaaaaac gattgatgcc gaaggagctt tcttgttcgg aggatcaatt
     1561 gcattggcgg gaattatcgg tggtgtattg gcacttgtga tgtcgcagat tatggcgact
     1621 tctactggag caaccggctc gacattaagt cttataggac ttttgtatat cgtgcgcgct
     1681 ggaacagatg tgtctaatct tgatttatca atgttcaatc caatgggatg gatttacttg
     1741 acctatcctt tcacagaaaa taactggcta ccattattat ttgctttgat ttttagtctt
     1801 gtttttaccg tacttgcgtt tgtgttggaa gaacatcgcg acatgggcgc aggttatctt
     1861 cctgaacgag aaggacgtgc gacggcgaag aaatcactac tttctgtacc tggtttgttt
     1921 ttcaagatta ataaaggagt aatgattggt tggctgatcg catttgtcgt tatgggagct
     1981 gcgtatggct ccatttatgg agacatgcaa gtctttcttg gcggaaatga actgatgaaa
     2041 caaatgttca ctcaatctgg cgtttccatt gaagaatcct ttacggcaac gatcatgatg
     2101 gtaatgattg gattagtcac aatcttgcca atcgcggtgg tcaataaatt atttgcagaa
     2161 gaaacaagac tgcatctgag tcaactgtat gtaacgaaga ttacgcgagg ccaattatat
     2221 tggacaacga tatttttagc tatttttgct ggagtcgtag gtattggctt agcatcagcg
     2281 ggattaggtg gaacggcgat ttctgcgatg aaaaatgaat cgactatgga tctgaccgat
     2341 ttcttagctg ctggatacaa ttttctccct tccatcttat tttatattgg tttggctgct
     2401 ttagcgttag gctggttgcc aaaatttgga aaagtaatct atgcttatct aggctattcc
     2461 tttgctttga attatttcgg cggaatctta gatttgccgg attggttctc aaaaactgca
     2521 attcaaagtt ggattccacg cttaccgatg gaaaaatttg atggaacgat ttttgcagta
     2581 attactgtta tcagtatcgt cttcttattt gtcggctatt taggatacaa acgccgtgat
     2641 atggtagaag gcgcttaaaa attgaatttc taaaaaaccg ttcatattta cgaaagtcga
     2701 tttgagcggt ttttttatgg cttcaattgt caaattaagt gcaacgattc attaaaaaat
     2761 acttcataag gggttttcca atttagacat ttccgtggtc gcgtgtttaa tttattcgcc
     2821 cattcttgaa tttgactatc gaaaatacct gaaatatctt cacctttggg tgaatattct
     2881 cgtaataaac cattagtatt ttcgtttgta ccacgttgcc atggagcatg tggatctgga
     2941 aaatacactt tagtgccatt taattctagc gttattcgac tatgttggga gaattctttt
     3001 cctctatcgg gtgtaacggt tttacattta tcttctccta catttcttag tagattgatc
     3061 atacaatcaa ccacattttt agagattttc ttttcaacct taccaagtag ggagaaacga
     3121 gatctacgat cgactagagt tacaatacga gccctacctg ttttaccggc tacagtatca
     3181 gcttcccaat taccaatctc aaggcgctca ttggcagcag atggcctttc atggatctta
     3241 ttgtatgcag atgatccaat gacagctagg gcaccaccaa aacaagcgca tgatattgtt
     3301 gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata
     3361 aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt
     3421 ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg
     3481 actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac
//
LOCUS       DACCVU010000192         3273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCVU010000192 DACCVU010000000
VERSION     DACCVU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.200"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            90..932
                     /locus_tag="IVW29_002878"
     CDS             90..932
                     /locus_tag="IVW29_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7840312.1"
                     /translation="MNANSIVVGNFKGGTGKSTSTQMLGFVNAFYKKRKTLLIDLDPQ
                     ANASEVMSFTADNLGNHFYQENGFPKTIWNVLYDGSLDGAVLPLIENLDLIAGNIAMS
                     DYSDFMSAKFPNDKLAQFEYFANILAPLKEEYDEIFIDVPPSLGTIVQSAMYFAEYVA
                     IMLQTQPKSIRGAADYIDYMEFFTERYNTQLGIAGIIPFMLDTKTSTESYMYNEAKKL
                     YGDNLINTIVFRSARLIRYDETGITLETKKNGELKKVDANPQNTFISILDELNEHISW
                     FEEE"
     gene            935..1489
                     /locus_tag="IVW29_002879"
     CDS             935..1489
                     /locus_tag="IVW29_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840313.1"
                     /translation="MAERQSKLFPNKKARPRTNGMKDMEEFTKSLSISSADNSAPKVN
                     TTEKHSDDNKNLKNPNKSHKGKAGRKAEYKDPRLKKDLNTKISLSTKLRIQRLISRKF
                     DNKSEGDVIDIALDYLVSSFDRDDRDSLYKAYKEDMESMIPIIQEKNEKAKKQGKLTL
                     DITDEINQQTLKEQKEKWVSAKFE"
     gene            2137..3222
                     /locus_tag="IVW29_002880"
     CDS             2137..3222
                     /locus_tag="IVW29_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7840314.1"
                     /translation="MSTEFQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT         1340 a          452 c          496 g          985 t
ORIGIN      
        1 ttgattacga gatacttatt tacatttaca gcagcaagtt gtatacttaa tttgaataaa
       61 agatacttaa tcaaaaggag cgtaataata tgaatgcaaa ttcaatagta gttggtaatt
      121 ttaaaggagg aacaggaaaa tctacttcta ctcaaatgct aggctttgta aatgcatttt
      181 acaaaaaaag aaaaacattg cttatagatc ttgatccaca agcaaatgct tcagaagtta
      241 tgagtttcac tgctgacaac ttagggaatc atttttatca agaaaatggg tttcctaaaa
      301 ctatatggaa tgttctttac gacggtagtt tagacggcgc tgtccttccc ctaattgaaa
      361 atttagattt aattgccggt aatattgcta tgagtgacta ctctgatttt atgagcgcta
      421 aatttccaaa cgataaatta gctcaatttg agtactttgc gaacattcta gcacctttaa
      481 aagaagaata tgacgaaatt ttcattgatg taccaccttc actcggaact attgtacaga
      541 gtgcaatgta ctttgctgaa tatgtagcta ttatgcttca aacacagcca aaatcaatta
      601 gaggggctgc tgattatata gattatatgg aatttttcac tgaaagatat aatacacaac
      661 taggtattgc tggaattatt ccatttatgt tagacacaaa aacaagtaca gaaagctata
      721 tgtacaatga agctaaaaag ttatatggcg ataaccttat caacacaatc gttttcagaa
      781 gtgctagatt gattagatac gatgaaactg gtattacatt agaaacaaag aaaaatgggg
      841 agcttaaaaa agtagacgca aatccacaga atactttcat atctatttta gacgaactaa
      901 atgaacatat tagttggttt gaggaggaat aaagatggct gaaagacaat caaaactatt
      961 tcctaacaag aaagcacgtc caagaacgaa tggtatgaaa gatatggaag aatttacaaa
     1021 atctttatct atatcttcag ctgataattc cgctcctaaa gttaatacaa cagaaaaaca
     1081 ttctgatgat aacaagaatc taaaaaatcc aaataaaagt cataaaggta aagctggcag
     1141 aaaagctgaa tacaaagatc caagactaaa aaaggatttg aataccaaaa tctctttaag
     1201 taccaaatta agaatccaac gccttatttc aaggaaattt gataataaat ctgaaggtga
     1261 tgtaattgat attgctcttg attatttggt atcttcattt gatagagacg atagagattc
     1321 attgtataaa gcatacaagg aagacatgga gtcaatgata ccaattattc aagaaaaaaa
     1381 tgaaaaagct aaaaaacaag gtaaactaac actagatatc accgatgaaa taaatcaaca
     1441 aactttaaaa gaacaaaaag aaaaatgggt atcagctaag tttgaataga agatacttaa
     1501 aacaacataa actattgatt gaagtatctg gtaatattga ttttaagata cctcaatcaa
     1561 tagttttttc tttaaaataa gcatttatgt ttgaataaga gatactaaaa tcaaataaaa
     1621 gtatctttta atcaacaaac tagtttgaat aaagttactc ataatcaaat tataaatgat
     1681 ttttagatac tacaatcaga tagaaaccct attaaattaa ctttcttaat tgattaaaag
     1741 atactaaaat gagattattt tgattttagt gccaaaaaac aaactaaaat ttttgatttt
     1801 agtatcctta aatcaaatta aatgtatatt acgagtcact ataatcaaag gtaatttttt
     1861 acgaatattg tcttctcaat aaaaagttct tgattataag atctttcaat cagatttatt
     1921 tttgaataag gtgtcttcca atcaaaagta ataactgatt taagtatctc ttattcaaaa
     1981 tatagttgtc tgaaaataat aaactgttaa acttattttg aagcctatat tattcctgat
     2041 ttgtatatcg aaattcatta gctatataca tttaaagaaa tctaattata tttttgataa
     2101 taaaaattgt acggatacta gaaaagaggg agtttaatgt ctacagaatt tcaatctcaa
     2161 aaaatgatta ttaaaaattg gggatataaa gcaacaaatg tttatattga aaaatattat
     2221 aaatttccta gttttttaat tcagaattct aaatattcaa aaatgaaaga cagctcaaaa
     2281 atagcttata tgctttttaa aaatgaattt cgtagagctt tgcatgaaaa atggatcgac
     2341 gatgatggac tattatattt ggaatttaca caagccgatc taatggataa attaaattgc
     2401 tatcaaggca aagtaaaaac cattttaaac gagttagaaa catataattt gatttctgtc
     2461 gtaaaaggag aatttaaccc aaaaacgaaa aaaaatgaaa aaaataaata ctatttactt
     2521 caacctgaaa taagtgccga tgatcttttt attcaaaatt ctttcgatga cgatttaagc
     2581 gaaactattg ataaatcaac ctcttctcgg aatgcggaaa tcgcacgtcg agaggaaact
     2641 gctaaaaccc ttgataatac tggaaatgcg gaaatcgcac gtcgagaaaa aacagctgaa
     2701 acccttgata acactggaaa tgcggaaatc gcacaagatt attatattaa taaatcttta
     2761 gacactaaaa gacacttaaa agacactgaa acagaccaat tacaaaatca agttttgcta
     2821 gataacttcg ttgaaatcat gaaagaagac agcataaaca catttgtacc agaaaaggta
     2881 ttaagcttga taaaaacatt ttctaacact tacgaagaag cccaacaaac tgtcaccaca
     2941 atacacaacg caaaacataa agccgaggaa cttgagaaga cggtagttgt gtatgaagaa
     3001 cttgaatctt atggaattga tgctgacaaa ggattatata tgactttact gaaagcttat
     3061 caaaaacaaa aaactgaaaa agtaaacaat ttgcagaatc taattttcgt ttatgtaaaa
     3121 aattggtttg tcgaaaaagc tattgcggca aagcttgcaa acgaacaaag agaaacattt
     3181 aacgaattac caacagttag tacggaaaat tggttagagt agttgataaa aagcaattac
     3241 tataaaaatg atcaatttta aattagaact ttt
//
LOCUS       DACCVU010000193         3269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCVU010000193 DACCVU010000000
VERSION     DACCVU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.201"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(339..707)
                     /locus_tag="IVW29_002881"
     CDS             complement(339..707)
                     /locus_tag="IVW29_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7840315.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            857..1339
                     /locus_tag="IVW29_002882"
     CDS             857..1339
                     /locus_tag="IVW29_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7840316.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1376..2386
                     /locus_tag="IVW29_002883"
     CDS             1376..2386
                     /locus_tag="IVW29_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7840317.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2501..3214)
                     /locus_tag="IVW29_002884"
     CDS             complement(2501..3214)
                     /locus_tag="IVW29_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840318.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         1108 a          520 c          637 g         1004 t
ORIGIN      
        1 ctctaaagaa attcgttctc tcctaaagtg atattatatt taaatatacc tgaaaataat
       61 gggcagtatt atgaaagatc actgatattt tctcgtattt tttccttatt caaaagtttt
      121 gcaggaaaat tgctgaaaaa ataattttac taagtagaaa attcaaagcc attcaatcag
      181 tgattttttt cagcacaaga aagactcctt ataaaaatag cagatacttt gtcaattaag
      241 tgtttaacca gggatgataa aaaggatagg attgaacaaa acctgtataa ggtgatgaat
      301 agtttcctcg ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa tttatcatga
      361 ccttttttat cttcaatatt aaatgaaatc aattgttcaa tcaattccca gttgattaca
      421 cttttccaag gaactttgat gacgttatcc gtatatacgt agcctgcttc ttggatattt
      481 ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa
      541 ctaaatgcaa taataaaggt accatggtca gtaaacatag gttggttcca ttttacagtt
      601 gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat cttttgttga
      661 tgttcttttt ctttgatttg attgatgatc ggctgaaatt tttccattct aaaacgcctc
      721 cattaagtgg tttgatttat tttaccagaa aaacaatagt tatgtataaa aaacgaacaa
      781 aacacaagta atgttgtaac cgctatattt atatgataag atgtctcaaa taataaaata
      841 gaaaagagac ataaaaatga ttgacttggc aaagaaattc ttacacgaaa atatatattt
      901 tgagttgtat catcatcgag ccatttatac aaatgaagat gctttggtaa taaagaaaga
      961 acaaggattt acaggtacag agaccaaaag cttattctta aaagataaaa aagataataa
     1021 ctacgtatat ctgactttta ctacgaaaca aacggatttc aaagcattag ggaagatcgt
     1081 cggtaaaagg ctttctattg tttcatcaga acgcatggaa gaacaaacgg gacaaaagca
     1141 gggcgctgtt tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct
     1201 cttatcaaat gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca
     1261 aacggttgac ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc
     1321 aaacgaaaaa aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat
     1381 aagttatcca ttattagaaa gaaatgcaac gattggggtt actgcaccat cctcaggcgt
     1441 atcagaggaa caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt
     1501 ccaagtagtt actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag
     1561 aaaacgagca gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc
     1621 gacttgggga ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca
     1681 accaaaatgg atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa
     1741 aacagggata gccactgcac atggaactaa ccttattgat ttaagaggag agtacttgga
     1801 tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca
     1861 gcaatcttct gaaaagtatc aaaaagtgtg gccccatgag cattcatcat cttctgtttt
     1921 ccatctcact gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg
     1981 acgattatta ggtggttgta ttgatacgat caggcattta atcggtactc cttatggaga
     2041 tgtccaatct tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa
     2101 ctgtgagcta tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggctg
     2161 gtttgatggc agctcgggga tcatgttcgg tagaagtgcg gcagaggatt tgttagatta
     2221 taaaatgaag gatgtatacg aagatctgtc aaaggaatta aaactaccga tcttgtatga
     2281 tattgattgt ggtcatgtac cgccacaact gacattgatt aatggtgcct atgcagaagt
     2341 agcggtagaa gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg
     2401 ccttggagtc tatcaaagac caataaatct aaatagaaaa gaaagtgaaa gaagctgaca
     2461 aaatgctgtc agcttctttt tgtataacat attagaaagt ctaattatct ataactgtca
     2521 catcgattaa ctgtttcacg tcattgggat ttcctgtagg aatagctact acagatgcat
     2581 tatcaatgat ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag
     2641 gcatttgcca atgtgaagga actggataac tagtgattgt cccgtcatgg ttacttgaat
     2701 acactaaaac tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga
     2761 ctgcactccc tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta
     2821 attcgaaatc acccgatgta tctctaaaag tattacctct aatagctagc caaacacgtg
     2881 cgtactcaat ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg
     2941 aggaggttgt gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag
     3001 aagaagtatt gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag
     3061 ctgatttctg agtacttgat ttttcagctg aactggtttg tctgattgaa cttttgtctg
     3121 tggtcgcaga tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag
     3181 agaccaacaa taaaataaaa cctaactttt tcatataaaa tacctcatta tttttttaat
     3241 accagtatag cataatcctg aatttttga
//
LOCUS       DACCVU010000194         3251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCVU010000194 DACCVU010000000
VERSION     DACCVU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.202"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(553..2166)
                     /locus_tag="IVW29_002885"
     CDS             complement(553..2166)
                     /locus_tag="IVW29_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7840319.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="IVW29_002886"
     CDS             complement(2307..3215)
                     /locus_tag="IVW29_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840320.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1046 a          558 c          573 g         1074 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt t
//
LOCUS       DACCVU010000195         3246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCVU010000195 DACCVU010000000
VERSION     DACCVU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.203"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            106..414
                     /locus_tag="IVW29_002887"
     CDS             106..414
                     /locus_tag="IVW29_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7840321.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="IVW29_002888"
     CDS             441..740
                     /locus_tag="IVW29_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP7840322.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..1465
                     /locus_tag="IVW29_002889"
     CDS             914..1465
                     /locus_tag="IVW29_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP7840323.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1611..1904
                     /locus_tag="IVW29_002890"
     CDS             1611..1904
                     /locus_tag="IVW29_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840324.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1897..2193
                     /locus_tag="IVW29_002891"
     CDS             1897..2193
                     /locus_tag="IVW29_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840325.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2264..>3246
                     /locus_tag="IVW29_002892"
     CDS             2264..>3246
                     /locus_tag="IVW29_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7840326.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMT"
BASE COUNT         1220 a          454 c          650 g          922 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat tggcagatag
     1441 tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat aagtagtttt
     1501 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1561 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1621 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     1681 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     1741 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     1801 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     1861 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     1921 ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc gaatatagat
     1981 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2041 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2101 gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg ttgtcatgct
     2161 ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat taattgactg
     2221 ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa aatggttgtt
     2281 attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata cgaggactaa
     2341 agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc aatcaactga
     2401 acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat
     2461 tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat ctactcaact
     2521 agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa gctacaatcc
     2581 tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg atggaaattg
     2641 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2701 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2761 gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2821 tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac agttaaaaca
     2881 aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag aagaatctac
     2941 tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt caagttggga
     3001 agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt ctcctgataa
     3061 aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3121 tgaaaatgga agctactatt caattaatat tgtaacggat gataatggaa atacaacaat
     3181 ggctggtgga aaacaattcc gtgtttatac agatggaaga atttctcaac gtattggaat
     3241 gactaa
//
LOCUS       DACCVU010000196         3245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCVU010000196 DACCVU010000000
VERSION     DACCVU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.204"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..341
                     /locus_tag="IVW29_002893"
     CDS             <1..341
                     /locus_tag="IVW29_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP7840327.1"
                     /translation="LYGKRGTMENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYN
                     LVNFLKSIGFDKVNQGMTIHSIRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVF
                     ERLRRSKQWI"
     gene            532..2019
                     /locus_tag="IVW29_002894"
     CDS             532..2019
                     /locus_tag="IVW29_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP7840328.1"
                     /translation="MNYSGFFEVEASIKVNQGSFKGVLSPTQRFKSQEELLSFVSSKQ
                     AKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKL
                     LSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAII
                     PTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGK
                     TPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKETQPPKAFTEGTLLT
                     AMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKL
                     LCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFIVHLLEAVPNDIEK
                     LNFSDYQVQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLT
                     KTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            2164..3018
                     /locus_tag="IVW29_002895"
     CDS             2164..3018
                     /locus_tag="IVW29_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7840329.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYKNVFFEMLERI
                     IQLENEKE"
     gene            3082..>3245
                     /locus_tag="IVW29_002896"
     CDS             3082..>3245
                     /locus_tag="IVW29_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7840330.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEV"
BASE COUNT         1248 a          493 c          604 g          900 t
ORIGIN      
        1 ctctttacgg caagcgagga acaatggaaa atttcatcaa agaagcgaaa ggtggctttt
       61 acttcgataa aacagatagc ccgcgtttct tagaaaatca tgtcagaatg atgatcagtg
      121 tccttgcata caacctcgtt aattttttaa aaagcattgg ttttgataaa gtgaaccagg
      181 gcatgacgat tcattccatc cggctgatat tgcttaaagt agccggtaaa cttgttcaga
      241 ctgggagaca ggtctatctc aaactgtcaa gttatcatgt gtatcaaact gaattttata
      301 aggtttttga acgcctgcgg cgatccaaac aatggattta gactagttat cacacaaatt
      361 ttttaaccga ttctttttca agggattcgt ctgcccttaa atagacaaat aatttttatt
      421 atgatcttct tccgtaggca tcctgttcga aaacacatta ttttggaaga aagtatctgc
      481 ctaatttaaa aattggcaat ttgttcaaaa aaacaggtta aatttagagc tatgaattat
      541 tcaggttttt tcgaggtgga agctagtata aaagtaaacc aagggtcgtt taagggcgtt
      601 ctaagcccca cacagcgttt taagagccaa gaggaacttt tatcctttgt ttcttctaaa
      661 caagctaaaa taggcaatca agaggggata attgctgatg ttcaaaccaa agagaagaaa
      721 acgaatagtc cgagtttgtt ttctttaagt agtttacaat caaaagtcaa tcagctttat
      781 aaagcgacag caagccaaac tttaaaagct atgcaaggac tgtatgaagc aaaattattg
      841 agttatccaa gaacagatac accatttatt acagagaacg aatttgctta tttaaaagca
      901 aattttggca aatatagcgg ttttttagga cttgatctcg aaatggttca aacagagcct
      961 agaaagcgtt atgtggacgg tagtaaggta caggaacacc acgccattat cccaacaaaa
     1021 caagtaccta ccgaatctgc attagcaaaa atggacgatt tacaacgaaa aatatatgct
     1081 ttagtcgtta aaacgaccgt tgccatgttt ttacctgatt acttgtatga agaaaccaag
     1141 atacaaacga aagtagctga tttgctgttt caatcgatcg gaaagacacc aaaacaagaa
     1201 ggttggaaaa ttcttttcaa acaacaaacc aaagaagaaa aagaggacgt tcaaacgtta
     1261 cccttggtta tcattggaga acatgccgag gttgacgtta agagtgccga aaaagaaaca
     1321 caaccaccga aagcttttac agagggtaca ttattaactg ctatgaaaac cgctaataaa
     1381 acggttgatg atgaagaagc aatcaagatt ttacaagaag tcgaggggat cggaacagaa
     1441 gcgacaagag caagtattat tgaagcgtta aaacaaaaag aatatatcca agtgattaag
     1501 aataagcttg ttgtaactga aaaaggaaaa ttattgtgcc aggcagttga aagtcagcac
     1561 cttttaacga gtgctgaaat gacggctaaa tgggaaacgt atttaaaaaa aatcggtaaa
     1621 agagaaggca atcaagaaaa ctttattaat aatatcaaga agtttatcgt tcatttactg
     1681 gaagctgtac ctaacgatat agaaaaacta aatttttctg attaccaggt acagaaagaa
     1741 aaagaagctg aaaaaagtat tgtaggaaaa tgtcctaagt gtggtaacaa tattgtatta
     1801 aaaaaatcgt tttatggttg ttcaaattat cctgaatgta agtttacttt agctgaacat
     1861 tttagaaaga aaaaactaac caaaacgaat gtaaaagaat tactggaggg aaaagaaacc
     1921 ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca atgccgttgt aaaaatcgga
     1981 gaaaagggat atattgattt tatctctttc tcaaaataaa cataaaagcc ctttaaacag
     2041 ggcttttata tattaatcac aaatcactta tcacaaatca caagtgatta atcacaaatc
     2101 acttgtgatt tatgattcct aatgatacaa tattactata caaaaaaaga atggggcgta
     2161 gttatggaga aggaagaact aaaaatactt gaagaattaa gacgtatttt aaacagtaaa
     2221 aatgaagcaa ttgttatctt aaacaattat tttaaaggtg gtgttggaaa gtcaaaatta
     2281 tcgactatgt ttgcttactt gacagacaaa tttaatttaa aagttttaat gatcgataag
     2341 gacttacaag caacattgac aaaagactta gcaaaaacat ttaaggtaga attgccacgt
     2401 gtcaattttt atgaaggact gaaaaatgga aacttggctt cttctattat tcatttgact
     2461 gataatttag acttgattcc tggcacgttt gatttgatgt tactgccaaa attaactcgc
     2521 tcatggacgt ttgaaaacga aagtagattg cttgctactc ttttagcacc tttaaaaagt
     2581 gactatgatc tcattattat tgatactgta ccaacgccaa gcgtttatac aaataatgca
     2641 atcgtggcga gtgattacgt tatgatccct ttacaagcag aagaagaaag tacaaacaac
     2701 attcaaaact atatttccta tttgattgat ttacaagaac aatttaaccc tggactagat
     2761 atgatcggtt ttgttcctta tttagttgat acggacagcg caacgataaa atcaaacctg
     2821 gaagaactgt ataagcaaca taaagaagat aacttggttt tccaaaatat tatcaagcga
     2881 agtaataaag taagtacctg gtctaaaaac ggcattacag agcataaagg ctatgacaaa
     2941 aaagttttat ccatgtataa gaacgtattt tttgaaatgc ttgagcgaat tattcaatta
     3001 gaaaacgaaa aagaatagaa tcacaaatca caagtgattg ttgatgataa aataagagta
     3061 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agcaaaacga
     3121 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
     3181 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
     3241 gttat
//
LOCUS       DACCVU010000197         3234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCVU010000197 DACCVU010000000
VERSION     DACCVU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.205"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..523)
                     /locus_tag="IVW29_002897"
     CDS             complement(<1..523)
                     /locus_tag="IVW29_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HAP7840331.1"
                     /translation="MENENRTDWTLPRKNLNPKTKQPYKRGRNWWIVVYPESLPENWK
                     EIISTEPVAISPLHDKDVNADGTKKKPHYHIVFNYKGNKSFEQMDEMARVLRAPIPER
                     ISGLTGAVRYLTHMDNPEKYQYDNTEIQVFGGFDLESCLALSTGDKRQALKEMLGFIS
                     DNNIMHLKDFADYC"
     gene            complement(647..802)
                     /locus_tag="IVW29_002898"
     CDS             complement(647..802)
                     /locus_tag="IVW29_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein repA"
                     /protein_id="HAP7840332.1"
                     /translation="MVEVEKKKVTLSLPVESNDKLEKMAQKYGMTKSGLVTFLINQAD
                     DKGTIFK"
     gene            complement(1346..2557)
                     /locus_tag="IVW29_002899"
     CDS             complement(1346..2557)
                     /locus_tag="IVW29_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP7840333.1"
                     /translation="MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNY
                     DLVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFF
                     DTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQ
                     DELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSND
                     KAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVI
                     KSYEELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEK
                     ENKSLKNEIKGIYKGFNQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNE
                     TKTRDRDELSR"
     gene            complement(2810..>3234)
                     /locus_tag="IVW29_002900"
     CDS             complement(2810..>3234)
                     /locus_tag="IVW29_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HAP7840334.1"
                     /translation="NLGYWEKLNPLYTVFNKETEKFSNIWTESNVSFNSFYNSYKSDL
                     LEYKDKNEMFPKKPIPENTVPISMIPWIDFSSFNLNIGNNSRFLLPIITIGKFYSKNN
                     KIYLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
BASE COUNT          968 a          654 c          426 g         1186 t
ORIGIN      
        1 tacaataatc agcaaaatct tttaaatgca taatattgtt atccgaaata aaaccaagca
       61 tttcttttaa cgcttgtctt ttatcaccag tagataacgc taaacaactt tcaaggtcga
      121 atccaccaaa cacttgtatt tctgtattat cgtattgata cttttcagga ttatccatat
      181 gcgttaaata tctaacagca ccagttaaac cacttattct ttcaggaatc ggtgccctta
      241 aaactcttgc catttcgtcc atttgttcaa atgatttatt gcccttgtaa ttaaaaacta
      301 tatgataatg cggctttttc tttgttccgt ctgcattaac atctttatca tgtaatggac
      361 taattgccac aggttcagtt gaaataatct ctttccaatt ctctggcaaa ctttcaggat
      421 aaacaacaat ccaccagtta cgacctcgct tataaggctg tttagttttt ggatttaaat
      481 tctttctagg caatgtccaa tctgttctat tttcattttc catatttttc tacaccttcc
      541 tagttactct actaggaaaa caagggaaaa tttgataaac ttgagttgcg aaatcaaaga
      601 aaaccaaatc ttgcgagccc ttgctatctt cctagcaggg ctttttttat ttaaaaatag
      661 tccccttatc atcagcttga ttaattaaaa atgtaaccaa acctgattta gtcattccat
      721 atttttgtgc cattttttct agtttgtcat tcgattcaac aggtaaagat aaagtcactt
      781 ttttcttctc aacttcaacc acaaaatgca cctcaattct cttcaaaatc tcttttacac
      841 tgtaattata ttatacatac gacaggaata caagatttta caaaaaactt tttttcacct
      901 cgaaataggt gcttaaaacg ttcatataaa ggtatttaca agacatttaa tctttttatc
      961 tatttggaca atggacaatg gacacatggg gggtgtcgta gtgcccccat gttttttcgc
     1021 tccaaaaatt cccatgtttt ttcgccccct gctcccacta aatttccaaa taataaaacc
     1081 gtgggctatt cgcccacacc caacaagcgc aaagcgcttg accttgcctc tgaaaagcaa
     1141 cttgataact gcattcgttt ctttccgtcg tcttagcaaa agctaaacaa ctcaaaaaaa
     1201 gagatcaagg gtaaatacac tcactacgtt cgcccttgac ctcttttatt tcgctgtttt
     1261 ttacgcattt gccgacgaaa agtaaacaat atgcagaatt accctagttt tgataaaaaa
     1321 gcgcttagaa cgcaaatatg agcttctaac gacttaattc atccctatct cgtgttttag
     1381 tttcgtttct atgaattttt tcaaactctc caccttttac aaactcttta acttcgctca
     1441 taaggttatt catcagcttt ttagcttgtc caacagttgc accaagggta ttttccataa
     1501 actggttaaa acctttgtaa atgcccttaa tttcgttttt aagcgactta ttctctttct
     1561 ctaattcttc aagtgtttca atattctgat tactcttacg taccaaccta ttgtaatctt
     1621 tcgctaattc gcgatatttc tctttaactc tagtgatttc tttaggtaaa tcagtttccg
     1681 cataattttt tagcttttca tttgctttat atagctcttc atagctttta atcactgcat
     1741 ttaatttact ttcagggata attacatttc ccgtttttcg ttcttcattt ttcataaatt
     1801 catgtcgcag cttaccaatc ccaatggttt cgccacttgg tactttgacc gttttcgttt
     1861 cgtcttttaa atctaaactt tctttcttga ttgttggtgc tttatcgttc gataacgcta
     1921 aaacttctga ctttcgctgt tccagcgtgg tagttatctc ttttaattcg tcctttgcct
     1981 ttttatattc aggcaccgtt aaatttttac gctctgaccc tttttcgcca cgttcaagct
     2041 caaaaccacg ctcatttaaa tactttggca attcgtcttg taccgcttgt aaggcttgac
     2101 gattaaaaac agtctttgct gatagcttgt gttcgtcatt aaacggcaca attcctaagt
     2161 gcatatgcgg tgtcgtttcg tctaagtgaa cttgagcgta gcggatatta ttatcgccaa
     2221 atttgtccgc aaaatatgat ttcgcagtat caaaaaaatc tttaatttct ttatcgtctt
     2281 tatctttaaa aaatgggttg tcagaagtta tgatccattc gtttattaat acagcatctt
     2341 ttctgacagc acgactactt gatttattgt cgttaataaa ttgctcaata tctcgtttat
     2401 aattttctgt ccgattgacc aaatcataat ttaaatgcga ccgttcaaca tcaatatctt
     2461 tgttggaatg attgtctgta tttctctgat tatgattacc taccccgact aaatttcctg
     2521 attttacctt ctgcattctc gccactgcaa atgacatgta aacactccta acttcggcac
     2581 aacttccatg taaagtataa cccactagac ttatttacta cgtttcataa gtcattaatg
     2641 cgtgtgctct gcgaggctag tttttgtgca agcacaaaaa atggactgaa taaatcagtc
     2701 cataagttca aaaccaaatt caaaatcaaa acaccagcaa ccaaaaatgg tagttgctat
     2761 aaacttataa atatcataat tacttaagtt tatgaaagtt attttagatt taaatccatt
     2821 catttacact atcaactata ttttgaaatt cactcataaa taatgaaaca tggtaaccat
     2881 cacataccgc atgatgaact tgcaatgaga ctggtaaata gatcttatta ttcttactat
     2941 aaaatttacc tattgtaata attggcaata ggaatctact attattacca atatttaaat
     3001 taaatgaact aaaatcaatc caaggaatca tcgaaatagg aactgtgttt tcaggtattg
     3061 gttttttagg aaacatttca tttttatctt tatattcaag taagtcactc ttataactat
     3121 tataaaaaga attaaaacta acatttgatt ctgtccaaat gttagaaaat ttttcagttt
     3181 ctttattaaa gactgtatat aaagggttta atttttccca ataacccaaa tttc
//
LOCUS       DACCVU010000198         3148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCVU010000198 DACCVU010000000
VERSION     DACCVU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.206"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(49..2541)
                     /locus_tag="IVW29_002901"
     CDS             complement(49..2541)
                     /locus_tag="IVW29_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7840335.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2548..3015)
                     /locus_tag="IVW29_002902"
     CDS             complement(2548..3015)
                     /locus_tag="IVW29_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7840336.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          835 a          691 c          517 g         1105 t
ORIGIN      
        1 ttaatcaagt cttactatta taaaaattca gctacacagg ccaagtaact atagggaacc
       61 tactgtttcc tttttaggtc cgcgaacatt caatgtgatc ttaccttttg aagcacctac
      121 tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa gcctcgctca atcgatcttc
      181 aatttctttt tgaagtgcgc gacggattgg acgtgcacca tattcaggat caaatccagc
      241 tttcccgatc acatcgattg cagatggtgt gattttcact tgaatatctt gttctttcaa
      301 tcgtttgata atcgctttgc tcatgatttg gacgattgta tggatttcat ctttactcaa
      361 tgaatggaag acgacagtct catcgattcg attcaagaat tcaggtcgga aagctttctt
      421 taactcttct aagatacgtt tttgcatggc tttgtgatct ttagtaacat cttggacatt
      481 gaatccgaca tttttttctt ctcgaatttg tgtcgctccg atatttgaag tcataatcat
      541 gattgtattt ctaaagtcca cttttctacc tttagaatct gttaaatgac catcatccaa
      601 tacttgcaga agtaaattga aaacgtctgg atgtgctttc tccacttcat ctaagagaat
      661 cactgaatat ggtttttgac gaattttttc tgtcaactgt ccaccttcat cataccctac
      721 gtatcctgga ggagacccga ttaatcggct tgtactgtat ttttccataa attctgacat
      781 atcaacacgg atcagggcat cttcacttcc aaacatgact tctgaaagtg ctttggccaa
      841 ttctgttttt ccgacaccag ttggtccaag gaacatgaat gaaccaattg gacgatcagg
      901 atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa actgctttga cagcctcatc
      961 ttgaccaacg acacgttcat gcagaagtcc ttccagttct aacaagcgtt cgctttcttt
     1021 tttctccaac tgctgtagag ggacaccagt ccactccgat acaactgtag caacatcttc
     1081 ttctgttaca cgatcagaat aaccttgctc ctgttttgct tcaattgcag aaactttttg
     1141 cagcttttta cgtagtgctt tttcttgtct gcgtaattga gcagcatttt caaagtcttg
     1201 tttttggatc gctgcttcct tttcttgtac tagttcatcg atttctagtt tgatcacagt
     1261 tgattttgtt aagtgatcgg tttgatccaa acggactttc gctgctgatt catcgatcaa
     1321 atcaattgct ttatcgggta gttgacgaga agtgatataa cgaacagaaa gattgactgc
     1381 tgctcgtaca gcttcatctg tgatctccac tccatggtgc tcttcatatc ttgtacgtaa
     1441 tccttgaaga atcacttctg cttcttcagg agttggctca tctacttgaa tgcgagcaaa
     1501 acgtcgctct aatgcagaat ctttttcgat atatttttga tattcatcta gggtcgttgc
     1561 tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc aagatattcg aagcatcaat
     1621 tgctccttct gctccacctg caccaattaa ggtatgcaat tcatcaatga ataagattac
     1681 ttgaccatca ttatagattt cgtcgatgac ctttttcatt cgatcttcaa attcaccgcg
     1741 atatttcgta ccagcaacca aagatcccat atcaagcatc atcaggcgct tttcttgcat
     1801 gtcttctggc acttcaccta aaatgatttt ttgagctaac ccttcagcaa tggctgtttt
     1861 gcctacacca ggttcaccca ccagtacagg gttattcttt gtccgtcggc taagaatctg
     1921 gataagccgt ttcacttctc tcgaacgacc aacaactggg tccagcttgt tttcttttgc
     1981 taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt gttccttgcg gtgtttgccg
     2041 tttatcttgt acatttacat ttctacgacg attcattcct tttccagcag aatcaccagc
     2101 tagccccatt ttttgtttca gaacttgacg ggcttttgaa aggctgatac ctaagttgat
     2161 catgattcga gtagccatga tactttcatc ttttaataag ctcatcaaaa tatgttctgt
     2221 tccaactttt tgagaaccag ttttcttcac ttcgctgcta gctaaagcaa aaacttgttt
     2281 cattcttggt gaatatggaa gaaagactcc tttagggtaa gaacggatac caccataacc
     2341 aatcagatgt tcaatttctt cataaatttc cgtttcattc aaatccattt cacgtaatac
     2401 ttttccagca attccatttt gctcgaccac taaggctagt aaaacatgtt ctgaactaac
     2461 tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt tctaatactg cttgggctct
     2521 ttcagtaaaa agttcttcca tctaatctca cttcccatct tcataactta aacgttccag
     2581 aaatgctcgg agaatccgag cacgtgtatg atcttcatat tcattgaaat tcaatgtgtt
     2641 ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt gtaattattt ggtcttcata
     2701 aagtttttga ataatagcaa aagcattttt ctcactgatt gtatcatcaa ataactgatt
     2761 tatctgctca agtaattgtt ttttatctga aatccttact tttgcaattc gtatataacc
     2821 accaccacca cgtttgcttt ctactagata accacgttgg atggtaaaac gggtattaat
     2881 cacatagttg atttgtgaag gaacacagtt aaagaggtct gccatttcaa tccttcgaat
     2941 ctcgatcatt tcactatttt ccagaatttt ctttatatat gattcaatta aatccgaggt
     3001 attctgatgt gccatcttat tcattccctt tgactaatat tgaccttaat tataacgaat
     3061 ttgataatag aaagaaagaa gaacgctttt tgttaaataa atcctttaga atcaacaaaa
     3121 aaaaatacgg aatatactat tccgtatc
//
LOCUS       DACCVU010000199         3136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCVU010000199 DACCVU010000000
VERSION     DACCVU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.207"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(356..970)
                     /locus_tag="IVW29_002903"
     CDS             complement(356..970)
                     /locus_tag="IVW29_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7840337.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1576..1953)
                     /locus_tag="IVW29_002904"
     CDS             complement(1576..1953)
                     /locus_tag="IVW29_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840338.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2253..3134)
                     /gene="galU"
                     /locus_tag="IVW29_002905"
     CDS             complement(2253..3134)
                     /gene="galU"
                     /locus_tag="IVW29_002905"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7840339.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          952 a          651 c          485 g         1048 t
ORIGIN      
        1 acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca tcaacgtgta
       61 ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca ctacttttta
      121 tttttctgac actacatatt cttataactc atttctcata actgtgaaac aacttataaa
      181 aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt aaaataacta
      241 aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt tttcaaacgc
      301 tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg gataactatt
      361 ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc tgcagcaaaa
      421 agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc aaagtcaaga
      481 ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca aataatcgca
      541 aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta acaatgatta
      601 atggatgacc attttctttg atccactgag ctagttcgat gattgccggg tttcttcctt
      661 cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca ataattgcct
      721 gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc aatatacttg
      781 gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt gtgcggctag
      841 catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg ccagcttgaa
      901 gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag tcaaaataac
      961 ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac agctgcctaa
     1021 tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc tttgctgttt
     1081 caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca aataaaacaa
     1141 cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga ttaggtacaa
     1201 taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg tgtatgtgtg
     1261 tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt tttttattca
     1321 gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc aagaaaatac
     1381 aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga atggcttatc
     1441 ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa aagctttttt
     1501 ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc tttttttgcg
     1561 tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga gagaatctgt
     1621 actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc ttttcatata
     1681 tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca
     1741 actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat
     1801 aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag
     1861 caatagtggc aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag
     1921 cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt
     1981 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
     2041 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
     2101 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
     2161 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
     2221 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
     2281 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
     2341 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
     2401 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
     2461 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
     2521 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
     2581 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
     2641 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
     2701 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
     2761 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
     2821 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
     2881 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
     2941 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     3001 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     3061 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     3121 tttttgacct tcactt
//
LOCUS       DACCVU010000200         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCVU010000200 DACCVU010000000
VERSION     DACCVU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.208"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            59..409
                     /locus_tag="IVW29_002906"
     CDS             59..409
                     /locus_tag="IVW29_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840340.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            614..1462
                     /locus_tag="IVW29_002907"
     CDS             614..1462
                     /locus_tag="IVW29_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP7840341.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            1509..1703
                     /locus_tag="IVW29_002908"
     CDS             1509..1703
                     /locus_tag="IVW29_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7840342.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            1706..2908
                     /locus_tag="IVW29_002909"
     CDS             1706..2908
                     /locus_tag="IVW29_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840343.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
BASE COUNT         1145 a          560 c          583 g          840 t
ORIGIN      
        1 gccatctata ttttttaatc aaatcgtata aactttcgta taaaaaagga gaattgtgat
       61 gccaataaaa attaccgatc tcagtttacc aaaagaaaca ctcgaaaaag aataccaagt
      121 cgtcaatgtc actcgctggc aaaaggaggg cgaaattcta ggttggagct acgaatgtat
      181 cctaccaaaa cttcgctacg aaaaaatcag tgtcaaagtc aaatctgaac atcctgtgat
      241 cacgccggaa gaacttaccc aacaaggttt agccataatt acatttacca atttggtgat
      301 tgtaccttgg ggacgctcga atggacaatt tgtcagttat gggttatcgg caaccgctga
      361 tacggcaact ttgattccca gaaaacaagg taccactaca caaaactaac tatactatgg
      421 agtgttgggt ggttaggacg aaacggcggg gcagcgccca cagagccgtt tctgtccgtt
      481 aagaccgccc aacaccccat aggctagaag ggctgctacg caggccttcc cccttaactt
      541 gatttataga tcaacatcag gagaaaatga ctggttcttt gacaatcgaa tacgcaacaa
      601 ggagtgatga tttttgcaag cttataatca gaaaatcatt gaaactccca cgtatattga
      661 aatttatgat tatgaagcaa tcattactcc caaacgtaaa acgatcgaaa acgaagtaaa
      721 tctggaacga aaacaaaaat tagcttggat ttatgaaaat gatattactc ttgaaaaaag
      781 aaaaaactat gatgaattat ccgctcaagg acaatatgac agtttaaaac gcaaacagaa
      841 acattatcaa agtatgcgat ttgagattgc ccgccttgta gacacgaatt ttgacaatca
      901 aacgaaattt ttaactctca ctttcaaaga aaacattcaa gacattgagt atgcaaataa
      961 tgaatttaaa aaatttatca aacgactgaa ttatgacctc tataaaacta aaaaagcaaa
     1021 tttaaagtat ctcgccacgt gggaaaagca gcaacgcggt gcaatccact accatattat
     1081 cttgtttgat ttccccttta tctcatttaa ccggttaact acgatttggg gtcagggttt
     1141 cgttaagatc aacaagatcg atgtagacag tatcgaaaat cgcgggcgct atatcagcaa
     1201 atatttcgat aaagagctgg aattgaaaga gcataagaaa aaagcttttt tcaagtcccg
     1261 gaatctcaaa ctaccaactg tcaccaaaaa gatggtaaag aaaccctatg attccacgaa
     1321 tcaagatata ctatatacaa gcgaatatat agttagacgt ccattttttt acaatgcttt
     1381 agctttaaat gagttgggaa gtcctgaaca agtgatggat tttcaagaaa gtaccgttca
     1441 ttatacaaaa ataaagaaat aacactatta cccaatgata gtgttttctt tgaaagtgag
     1501 tttatacaat gaaaaataca atttgtcgaa aagatttaga ggctctagga tttaaaacac
     1561 atcaagctac cacaataatc cgacaagcaa aaacaagaat ggtaaatcag ggatatccat
     1621 attataataa taaaagagtt ggggtagtac cccgacaagt aattgaagaa attataggtg
     1681 ttcctctttc acaggaggaa taactatgaa aacaaaatac ccttgtgtgt atcaagataa
     1741 aaatggtcgc ttttcttatc tagttgagtt aggaacagat caagtgactg gaaagaggat
     1801 ccagaagaaa ggaacaaaag attctctagg aaaacgattc tcatcagctc gtgaagcaca
     1861 caaagaagtc atgcgtataa aaaatgaata catggttgta aatggatttg cgaattatga
     1921 tttaacttac aaagaattta tggagcagtc ttacgttcca cactacaaag ctagcgtaca
     1981 aaaaagtact tggagttctc gtgagtgtgg attgattcag attactgaat ttttcggtga
     2041 taagaagctt cgtgaaatca atgtgcgaga ttgtgaaaat tatcgaattt ggttattgaa
     2101 cgatagcgga tactcacaag catacagttc acttatgtac ggtatgtttc gtaaatcatt
     2161 agactatgcg gtcaccctgc aatttctgaa tgagaatatt tccaaaagaa caaaagcaat
     2221 tccaaaagga aaatcaatcg ttccttactg gacaaaagaa gaatttgaag aagttttggc
     2281 aaaaatttat aaagatgatt tctacgaaca tatgtgtttt ttaatgatct ggctatatta
     2341 catgactggt gttcgtgtaa gcgaaggatt agcacttaat tggaatgacg tggacttcaa
     2401 acgtaaaaag ttgagggttc atcacacctt ggaaatgaca aatcaaaaag attttattcg
     2461 gaaaccttac acaaaaactg aaagcggctt gcggatcatt tctctggacg atgatacaat
     2521 tgaagtgcta aaaaagtgga aagttattca aaaaaatcat ggtgtagaac aatttattct
     2581 tagctacact gacctcccac tctatcgttc aactgttcaa cgaattattc agcgttatgc
     2641 caaactagct aaggtacccc taatacaagg aaaaggatta cgtcattctc atgttagcta
     2701 cctaatcaat gagtttaacg cagacattct ggtcgtgtcg caacggttag gccattctag
     2761 ccctgaaatc actttaaagc actacgctca tctatggagc cgtaatgatg aaggaattgc
     2821 agaacaaatg acaggaaaca ttaaatttac ctgtgcaaca aaatctaaag ttgcaaactt
     2881 taatggaaat caagcagtaa aaatctaacc ctaccatttc cctaccaaaa tgaggaatag
     2941 gctgaaaaag cactgatata atagcttctt tacgaggcta ctcatattga gtgggaataa
     3001 gacgtaaata taatatggat ggggaatgcc gataaatcaa cgtttttcaa cgtaagcgcc
     3061 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
     3121 ctggaggt
//
LOCUS       DACCVU010000201         3106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCVU010000201 DACCVU010000000
VERSION     DACCVU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.209"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            4..1389
                     /locus_tag="IVW29_002910"
     CDS             4..1389
                     /locus_tag="IVW29_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7840344.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
     gene            1376..3010
                     /locus_tag="IVW29_002911"
     CDS             1376..3010
                     /locus_tag="IVW29_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840345.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         1061 a          453 c          528 g         1064 t
ORIGIN      
        1 aatatttgta cgattctgat tagtttttat tatggagaaa agttttttaa aaagatagat
       61 gctgcttttt tatttagttg tttatatact tttgcaactt atcgaacagt tgatatctat
      121 tatcgatccg caattgcaga agcaatcgct cttacgatta tcccagctgt tttatattat
      181 gcttatggat tgatattcaa tagagaaaaa aaagtcatcg ctttatctgt cagtataagt
      241 ctacttgtgt atactcatgt cttaactaca ctattgtgta gtgcattgat tgcttgtatg
      301 attatagttc gtttgttttt aagcaagcaa ataaaaaacg atataaaatt tgtggtcgtg
      361 aatttagtaa aggctgtttt tttgacttgt gcattaacta gctatttttg gtatcctatg
      421 ttgcagcaga tgaattatca agaaatacac agtcccttca aaacagattt gcagctagaa
      481 gcactttcta tttcagatag tatcattcaa gcgttaaata acgatattac aacatacact
      541 atggggttat taggattggt tgcgttgatt tttcctttat tcatactgaa agaaatggag
      601 aaaaaagaaa aaatcatcta tgttggcgta gtttctacat gggtatgcgt aactaattta
      661 tttccatggt ttttgtttca aaaaacaccg ataagcttac tccagtttcc ttggcgtatc
      721 ttaggtatcc aaatcttttt ttcttcaatg atcataacgc ttgtttttat gaaaaagact
      781 atgaataaaa aatattcatg gttaatcatt ggcgctactg tggtgtttct actcgtcacg
      841 tctatagcag caaaagaaaa ttacgcaaaa gttatccaat caaaacacgg tcatattgtt
      901 atcaatgaag aaacactccc acgttataca acatcagata tgggaggatt atacgattac
      961 gccccaacag aagcgataaa agaaaggcct cacttagaaa aacatgaagt aaaagtagga
     1021 gaatcgtggg aaaaagggaa gtataaagct gcagataata acattactta cacagtggca
     1081 agcaacaaaa agcaaaaagt gacagtacca gtatatgctt atttaggtac acaggtaaaa
     1141 gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg gcttagtgaa tgtggatgta
     1201 aatgctggag aaaataagat agaaattaca acaaaatata ctccaactgc tctcatggct
     1261 ttatggatca gtattctgac ttattgttta gtggtgtatt caagttttaa acgatttgca
     1321 cctaataagt tgaaagattc tcgatatgtg actaaaaagg agataaacaa gaagaatgaa
     1381 aataaataaa aaagtagtaa tattgagtgg attaattata gttagtagta tatattcttt
     1441 catctttttc aaaaatacac agagtattta taccactgga gatctttctt ttcacttatc
     1501 tcgaatcaag gggttatcga ctatttttac cagtccgatt aattatgaga cattcaatta
     1561 tactggatat ggtgtaaatt atttttatcc ctttctgact ttttttccag ctgtaatgtt
     1621 atattggatg acaaaaaatt tgattgtaag ctatattatt tacgtttggt tattgaactt
     1681 gtgtacaaca ctcgtagctt atcattatgg cgagcggttt ttaaaacaaa aaaaagcggc
     1741 atttttattt agttgtctct atattttctc tgcttatcgg acagtagaca tctactatcg
     1801 ttccgcaatt gccgaagcta tcgcaataac acttgttatc cctgtattat tttatgctta
     1861 tcaaatcatc tctggaaaag aagaaaagta tccgtctgta aagctagcat tgagtatgag
     1921 tttgctagtg tacagccatg tactatcaac attaatgagt acagcattaa ttatcatttt
     1981 tatttttatt cgtttggttt ctaaaggttt caagaatgca gattttattg ctatatttaa
     2041 aaaattattt agtgcggcag gaatgacatt ggttctgacg agtgcttttt ggtatccgat
     2101 gtttgaacaa atgctctacc aaaaaatcaa caaacctagc gtgacaaatt tatatgctca
     2161 tgcatctaat gtgttcgata gtctgactga agctatgaac aacgacttaa ctacttactc
     2221 aatgggacta gttggtctgt tatctttatg tattcccctg attttattta aaaaattaac
     2281 aaacatagaa aaaaagattt attatggtac gtgtttgact tggctagcta caactagttt
     2341 ggtgccatgg tatcttctgc aaaatacgcc agccaaattg ttacaatttc cttggcggat
     2401 cttaagcttg caaataatat tctcttctct gatattaacg atgatatttt tcaaaaatag
     2461 aagatacaat aagacaagag aactatttta tttatttatg actgttttgt tattaatcac
     2521 tttaactgtt tctagtaaaa aaaatttcaa tcaaaaaata atttctcagc cgggacatat
     2581 tgttgtaaac aaagaaacag tagaggcgta taccactaaa ggaatggatt attttttata
     2641 tgattattct cctatcgctt cattggagaa taaggagcat ataataaaac acgaagcaaa
     2701 gattggcagt caatgggtaa accttcctta taaaacaact ggtagttcta tcatatatag
     2761 agtaactaat gagaagagcc aagtaatcac acttcctgta tatgcttatt taggaacgaa
     2821 tgtagaaata aatcatcaac ctgtaaagga gcaggtgaat gctaatggat tggtacaaat
     2881 aagcggagtc aaaggagttg ctgatattaa agtaagcaca agttatacag taacgactat
     2941 tatttcttat cttgttacat taactgcata tttatgttta ttttttaaaa tagttttcca
     3001 taaaaggtaa attaactttt catttataaa ataaattgat taaaaaggag aatctgtcga
     3061 taaggattct ctctttttta tacgaaaaat ctgtaggggg atgaag
//
LOCUS       DACCVU010000202         3101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCVU010000202 DACCVU010000000
VERSION     DACCVU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.210"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            75..503
                     /locus_tag="IVW29_002912"
     CDS             75..503
                     /locus_tag="IVW29_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7840346.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IVW29_002913"
     CDS             490..732
                     /locus_tag="IVW29_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840347.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IVW29_002914"
     CDS             complement(1032..1247)
                     /locus_tag="IVW29_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840348.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IVW29_002915"
     CDS             1354..1668
                     /locus_tag="IVW29_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840349.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IVW29_002916"
     CDS             1772..2950
                     /locus_tag="IVW29_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7840350.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1025 a          468 c          624 g          984 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt g
//
LOCUS       DACCVU010000203         3089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCVU010000203 DACCVU010000000
VERSION     DACCVU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.211"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..93
                     /locus_tag="IVW29_002917"
     CDS             <1..93
                     /locus_tag="IVW29_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7840351.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="IVW29_002918"
     CDS             complement(151..1371)
                     /locus_tag="IVW29_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7840352.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..1996)
                     /locus_tag="IVW29_002919"
     CDS             complement(1391..1996)
                     /locus_tag="IVW29_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7840353.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="IVW29_002920"
     CDS             complement(2045..2977)
                     /locus_tag="IVW29_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7840354.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          851 a          644 c          542 g         1052 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttt
//
LOCUS       DACCVU010000204         3022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCVU010000204 DACCVU010000000
VERSION     DACCVU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.212"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVW29_002921"
     CDS             complement(287..649)
                     /locus_tag="IVW29_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840355.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVW29_002922"
     CDS             complement(723..1103)
                     /locus_tag="IVW29_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7840356.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVW29_002923"
     CDS             1185..2360
                     /locus_tag="IVW29_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7840357.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="IVW29_002924"
     CDS             complement(2436..3020)
                     /locus_tag="IVW29_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7840358.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          577 c          498 g         1005 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat at
//
LOCUS       DACCVU010000205         2990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCVU010000205 DACCVU010000000
VERSION     DACCVU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.213"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..773
                     /locus_tag="IVW29_002925"
     CDS             <1..773
                     /locus_tag="IVW29_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7840359.1"
                     /translation="TLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMV
                     SILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDN
                     SEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHG
                     NMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFI
                     DEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKGELI"
     gene            770..1840
                     /locus_tag="IVW29_002926"
     CDS             770..1840
                     /locus_tag="IVW29_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840360.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            1827..>2990
                     /locus_tag="IVW29_002927"
     CDS             1827..>2990
                     /locus_tag="IVW29_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7840361.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAA"
BASE COUNT         1004 a          371 c          486 g         1129 t
ORIGIN      
        1 ttacactttc ttcgtctaga ggtgcccagg tagtaatttg ttctttttta gtattatttc
       61 ttttgttgtt tgccaattca aaatttagaa agagcattag agaagtacta cctgtaaaaa
      121 tcaaatggat ggtaagcata ttattagttc tattggtata tttttctttt ggtactaatt
      181 atgctaagga ttttttagca acaattcctg tatcgattga tagaaaagta gaagttatag
      241 agtcaaatga atcagataaa actccacttc cagaaaaaga agtaatatta cagcattcta
      301 gtgataactc cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg cttaaagtta
      361 taagaccaac tttgttattt ggttatggag agaccgattt ctatgggaat agcgatgcaa
      421 aatttttgaa aaaagtagat cttgatacta cagatgtgaa tgaactaaaa agagcacatg
      481 gaaacatgca taatggattt cttcaagtac ttgtatcaag tggtataatt gcttttatat
      541 gtatctatgc tttctatgtg ttaaatatct ttagtttact caaaacattt tttagaagta
      601 aagtgaatta tgcgtttctt ggtattgttt taatttttat actatctatt tttatagatg
      661 aaatggtgga ggctcatgta cttttcaata agcgcgatgt catttcaatt gttttctggt
      721 attatttggg aagcatttct tacttgtatt taaaaaaagg agaattaata tgaagacagc
      781 tttcattgct tggactcctt tacatttgat taatatatta aatgtttatc aaaattttta
      841 ccgagaatca aaaggagatt tatttattta ttctgaattt gaaaacgcta gaagatatag
      901 tgagcgtata aaagaattag ggctatttaa tgatgttgtt tttgtgaatc accggaaaat
      961 cggttcttcc tttaaacgta aaattaactt gttaactaac cataatcaat ttttctcgga
     1021 tgatttatcc gtttatgatg aaatttttat acagggggaa aattatttca caaaaatact
     1081 tttctccaat gtttacaaaa aaaatccaga tgtaaaacta aattatatag aagatggttt
     1141 gggtacttat ttgaagaaga gtatattttc aacaagtaaa aacgttcaaa gagtttttaa
     1201 aatattaaat aaaaatagca tcttcaaaaa aaaaattgac gattattttg tctattgtcc
     1261 ggaattgtta ctttatgatg taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag
     1321 taaattgatg aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa
     1381 atgtatcttt tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat
     1441 tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc
     1501 tagagcaagg acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga
     1561 agtcttgcta cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat
     1621 ttctttttca ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt
     1681 aattttaaat gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt
     1741 tcttttctgt gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg
     1801 ggcagaatta agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat
     1861 tagttaagtc cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca
     1921 taactattcc tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt
     1981 tcacttcatt tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta
     2041 ataatgcaag ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc
     2101 tagctacact ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa
     2161 atgctttttt tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta
     2221 gttttgtaat tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt
     2281 tgattttatc ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga
     2341 cattatattt tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt
     2401 tgataggttt agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa
     2461 aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata
     2521 tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg
     2581 gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta
     2641 ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga
     2701 agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag
     2761 tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag
     2821 tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg
     2881 aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta
     2941 atatactatt aaacatgatt ttcataccta ggttaggaat aattgctgct
//
LOCUS       DACCVU010000206         2948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCVU010000206 DACCVU010000000
VERSION     DACCVU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.214"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            175..456
                     /locus_tag="IVW29_002928"
     CDS             175..456
                     /locus_tag="IVW29_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840362.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            458..874
                     /locus_tag="IVW29_002929"
     CDS             458..874
                     /locus_tag="IVW29_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840363.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(1211..1351)
                     /locus_tag="IVW29_002930"
     CDS             complement(1211..1351)
                     /locus_tag="IVW29_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7840364.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(1348..2322)
                     /locus_tag="IVW29_002931"
     CDS             complement(1348..2322)
                     /locus_tag="IVW29_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7840365.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          994 a          603 c          438 g          913 t
ORIGIN      
        1 aaaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa tttgatatca
       61 ctttttcgcc atcgtggcta gcttatgtat agtattttag caaaaaacag ctatacaaat
      121 actatattcg tatgatatac ataaattata cacaaatgcg aaaggagttt ttctatggac
      181 attttagttc ggaagatcga ttcaaaggtt attgcaaaaa tagacaaacg ttgccaagaa
      241 ctcacaaata aaactggcaa aaaatggagt cgaaatcgct acttaaaagt cctaatcgaa
      301 aacgattttg accacgcact tatgcattac aagaaagatc aattcgattt attactcgaa
      361 aaatttatcg ctattcaaga aaaaaatact gaaacgctga atgaatatgt tcgtacaaac
      421 aaccaactca ttgaaacact tatcggagga agataacatg agaaaaactt ctgttcaaaa
      481 acacgttacc ctttatccca gtcacgtgga cgttttagat tccattatcg aaagaaatcc
      541 agatatcaac aattactcgg aagccattcg ttttttatgt atgagttatg aacctgatac
      601 caacacgact actaccagca atcaagccaa actaaatgct atgggaaaag aaatttccat
      661 catgtccgaa atcattgcag aatttgctga tttatatcta aaagatacag gtattcttat
      721 tggcacaaat tctaaagtgt atcaagaaac gaaagaaaaa attgaaaaac aacttaatcc
      781 ctccaaacga aatcaaccaa aaaatcgtca aaaagaaacc aatcaaacat ttacttctaa
      841 aaatttcgac ccaacaaatt ttctaaaaag ataaaaaata aaccaggaga tttaaattcc
      901 tggtttattt tttgggtagg atcaccatct taatagcaac atttacaata agatagtaac
      961 caattgaatg aaaaaaacga atttcagaag aaataacaga aaaaaagata aaaacaaaaa
     1021 aattataaat acatgatttg aattcataat cgtaaaaaaa gctgctcgta cgagcagcta
     1081 aataagaaaa agattggtat gttttaagga taaaataagt ataacaaatt tttcacatag
     1141 agcaaagggg gcgaaaatcg cccccttttt ttcgttttaa aaccccaaaa cccaaagaca
     1201 taaatttcgg tcaattgacc actgcatttt tttctttata ctgagcaagg tcaaatcccg
     1261 aattgaccag ttgattgcgc accgtatttt tcgatacacc caattcatca gcaagttttt
     1321 ttagggtcat tttttctatc gaatcactca ttcagcacct acttccgtca catcaagtcc
     1381 ttgaagccat gtatctaatt ctcgtttcaa atcatctgcc gactccgaaa atgtttctcg
     1441 caaatgattc gtcaactcat cattttgact ttcttgcttt aacaattcac gatatttata
     1501 ttttgaattt ctatgaattt gcccccagta actatgttcc gttataagag cagtaatcat
     1561 cattctgtca gttactttca catcttctgg caaatagtga ggacttgcaa agctatctaa
     1621 actttcatga accattgcat gccaatcagt tgctttcccc cttcgcaatt gcaattctag
     1681 tcgccaccaa gtatcgattt ctttcggaac aactttccgt tttctttctt gttccaattt
     1741 tttgttatac atccgaatct gtcgttcact agcacgactt ccccaatatg ctgtttccag
     1801 tttcccagac cgaccgtaaa ttggtttgaa agaaacagga tcaaccaccc gatattgggt
     1861 aatgaaatca tctggcacgt caataatgtc acaagcaata tctgcacgtg agaaatgtgg
     1921 ttctaaaaat aaatcatgga taaagttctt catcgagccc gccagaaatt ggctaatttt
     1981 attggggtta aagtcaatcc gcccttttcc ctcttgaaac ttaagaatct caatataagc
     2041 tatattttcc tcatgttggt ctagtaattg ccagccatta tttccaaccg cttttaaata
     2101 tccgttacct tcgtttagac gcataagctg ttcaaaatct aaaatcaaca catcatgggg
     2161 agtgtgataa taaatatttt cccttaatgt accaacaatg gtaattcggt caattgacca
     2221 ttgcattttt aagggggtgc acatagtttg tgcatttaac cgcctgttag atatgcggtt
     2281 aagtttttcc cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa
     2341 gaaaaaagag atttttggaa agaatttcca gagttcacgt aactctaaaa atccatatcc
     2401 aaaaatctct agcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac
     2461 taggtgagta gtttccgtaa ttagccgaag ggtgcttcca acaccgttcg gcttttattt
     2521 tgctcttttt tctcgttttc tttgattata gcactcctct taaaaaaata aaaagacctc
     2581 agggaatttc attccccgaa ccccttgagc ctcctcctgc cgaaattatt tcacgcttcc
     2641 gctcttcatt tcattcagtt ctcggcttgc acaatctaac acgccgtttc gctaccagtc
     2701 gccttacagg ctccattctc gcttctctta cgtgttttaa cgattgtgcc gataactaaa
     2761 cgaaatgaac gctaaaacgt cttagaacgc aaaatacgcg cttataaaat catttcatct
     2821 tgattacatg cccgtcataa tcgttcagtt tagtttcatt ttcgtaaact ttttttattt
     2881 ttacgaacag aaaaaagtcg cacatcacca ttttataaag agcataagga aacgtgtacc
     2941 cactttct
//
LOCUS       DACCVU010000207         2886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCVU010000207 DACCVU010000000
VERSION     DACCVU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.215"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            287..694
                     /locus_tag="IVW29_002932"
     CDS             287..694
                     /locus_tag="IVW29_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840366.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVW29_002933"
     CDS             708..1109
                     /locus_tag="IVW29_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840367.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVW29_002934"
     CDS             1111..1482
                     /locus_tag="IVW29_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840368.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVW29_002935"
     CDS             complement(1518..1820)
                     /locus_tag="IVW29_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840369.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="IVW29_002936"
     CDS             complement(1916..2710)
                     /locus_tag="IVW29_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP7840370.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1022 a          411 c          439 g         1014 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataatt
//
LOCUS       DACCVU010000208         2870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCVU010000208 DACCVU010000000
VERSION     DACCVU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.216"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(133..2358)
                     /locus_tag="IVW29_002937"
     CDS             complement(133..2358)
                     /locus_tag="IVW29_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7840371.1"
                     /translation="MFAALGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVY
                     ADNTEISKIQTDLMRTTISSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEA
                     TGIGSSGGSTLTQQLVKQQILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSP
                     FGRNNKGQNIAGVQEAARGIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKED
                     LSAGLARKDFVLFSMYREGQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMN
                     EATRIIAQQLAEKDNADMSDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGV
                     ENYGYMLDDGAGSQVETGNVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIK
                     PVLVYGPAIDMGLVGSESRVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWS
                     NNIPAYHLYQDVLNSGGSKQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNG
                     FQALANGGVYEEGYIIDSITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKI
                     TTPFKDVISSLNGNLGNADWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDS
                     QARIRSANYLAKLINQAYQANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKK
                     SIQTPGKTVESLWAKNGPKKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKD
                     D"
     gene            complement(2585..>2870)
                     /locus_tag="IVW29_002938"
     CDS             complement(2585..>2870)
                     /locus_tag="IVW29_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP7840372.1"
                     /translation="FYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMT
                     IHSIRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          752 a          594 c          552 g          972 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag cttggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acctgaataa ttcatagctt
     2401 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
     2461 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
     2521 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
     2581 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
     2641 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
     2701 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
     2761 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
     2821 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag
//
LOCUS       DACCVU010000209         2811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCVU010000209 DACCVU010000000
VERSION     DACCVU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.217"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1567
                     /locus_tag="IVW29_002939"
     CDS             <1..1567
                     /locus_tag="IVW29_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7840373.1"
                     /translation="LILGACGGSKEKSKSSPDYELENVSFPLKEKVSLKMMSQSAPLA
                     PNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLPDAIFNAAAGDYDLL
                     NWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIYSMPWIEELGEGKES
                     IHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGNGEADEIPFSFINNG
                     GNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKYFNEMNNKGLIDKEA
                     FEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGPDGTKHVTRTNGMGF
                     SRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQANIFELKDGMLKHLP
                     LEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMHDTYLPYITEDNIYP
                     RVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYLAELERVGLEEWIQI
                     KQTGYDNFLKGAQ"
     gene            1568..>2811
                     /locus_tag="IVW29_002940"
     CDS             1568..>2811
                     /locus_tag="IVW29_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7840374.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKL"
BASE COUNT          918 a          458 c          651 g          784 t
ORIGIN      
        1 actaattttg ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga
       61 actagaaaat gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag
      121 tgcccctttg gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga
      181 gaccaatctc catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt
      241 ggatatcgct agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga
      301 tttattaaac tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta
      361 tatgccaaat ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc
      421 acctgatggt cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc
      481 gattcataca gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct
      541 tgaaatgccg caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga
      601 tccaaatggt aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa
      661 cgaagactta aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt
      721 cgttgataac gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat
      781 caaatatttc aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga
      841 ttggaatacg tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga
      901 taaagcaaat atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc
      961 agatggaacg aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt
     1021 aattactagt gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga
     1081 accgttacag tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat
     1141 ttttgaatta aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga
     1201 actaagacaa aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac
     1261 agtcactact atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt
     1321 gccatatatt acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga
     1381 tcgattagca aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg
     1441 gatcaccggc ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt
     1501 aggtttggaa gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc
     1561 acaataagtg ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat
     1621 gatgcgagga aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg
     1681 gtggataaat gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca
     1741 atactatcct tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga
     1801 tttggtccat tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg
     1861 cgggtgtttc tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg
     1921 ctgtcaagaa attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg
     1981 tattcatttt agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa
     2041 tgggctaata caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat
     2101 ggtagtagct actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga
     2161 tttgtatact tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat
     2221 gtgggaatgt cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat
     2281 acaaatcaag aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg
     2341 tgaggtaaac tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg
     2401 aatggatttt tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc
     2461 ttggatgcag atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg
     2521 ctctatgagt ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat
     2581 ctcaactgta tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga
     2641 cacatcagtc gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga
     2701 tttggagaaa gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa
     2761 actagcctat gacactgcta cacaggtttt tgaattggat cgtcagaaac t
//
LOCUS       DACCVU010000210         2791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCVU010000210 DACCVU010000000
VERSION     DACCVU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.218"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1350
                     /locus_tag="IVW29_002941"
     CDS             <1..1350
                     /locus_tag="IVW29_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7840375.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2791
                     /locus_tag="IVW29_002942"
     CDS             1484..>2791
                     /locus_tag="IVW29_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7840376.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1092 a          383 c          542 g          774 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat t
//
LOCUS       DACCVU010000211         2749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCVU010000211 DACCVU010000000
VERSION     DACCVU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.219"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(94..1188)
                     /locus_tag="IVW29_002943"
     CDS             complement(94..1188)
                     /locus_tag="IVW29_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7840377.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1377..2138
                     /locus_tag="IVW29_002944"
     CDS             1377..2138
                     /locus_tag="IVW29_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7840378.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2253..2693)
                     /gene="fabZ"
                     /locus_tag="IVW29_002945"
     CDS             complement(2253..2693)
                     /gene="fabZ"
                     /locus_tag="IVW29_002945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7840379.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          873 a          549 c          466 g          861 t
ORIGIN      
        1 taatttgaca aatcataacc tataaaaaaa caaaataaaa aagcagaaat aactggctat
       61 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
      121 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      181 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      241 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      301 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      361 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      421 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      481 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      541 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      601 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      661 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      721 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      781 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      841 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      901 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      961 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
     1021 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1081 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1141 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1201 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1261 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1321 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1381 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1441 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1501 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1561 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1621 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1681 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1741 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1801 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1861 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1921 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1981 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     2041 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2101 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2161 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2221 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2281 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2341 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2401 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2461 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2521 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2581 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2641 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2701 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagta
//
LOCUS       DACCVU010000212         2667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCVU010000212 DACCVU010000000
VERSION     DACCVU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.220"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="IVW29_002946"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="IVW29_002946"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7840380.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          955 a          466 c          593 g          653 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa aacttggaga taacatc
//
LOCUS       DACCVU010000213         2652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCVU010000213 DACCVU010000000
VERSION     DACCVU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.221"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            137..748
                     /locus_tag="IVW29_002947"
     CDS             137..748
                     /locus_tag="IVW29_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7840381.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IVW29_002948"
     CDS             821..1750
                     /locus_tag="IVW29_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7840382.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IVW29_002949"
     CDS             1908..2645
                     /locus_tag="IVW29_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP7840383.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
BASE COUNT          930 a          369 c          493 g          860 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aa
//
LOCUS       DACCVU010000214         2603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCVU010000214 DACCVU010000000
VERSION     DACCVU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.222"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..177
                     /locus_tag="IVW29_002950"
     CDS             <1..177
                     /locus_tag="IVW29_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840384.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IVW29_002951"
     CDS             190..693
                     /locus_tag="IVW29_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7840385.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IVW29_002952"
     CDS             753..1142
                     /locus_tag="IVW29_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7840386.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..>2603
                     /locus_tag="IVW29_002953"
     CDS             1129..>2603
                     /locus_tag="IVW29_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP7840387.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          774 a          430 c          599 g          800 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tgg
//
LOCUS       DACCVU010000215         2571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCVU010000215 DACCVU010000000
VERSION     DACCVU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.223"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..1025
                     /locus_tag="IVW29_002954"
     CDS             18..1025
                     /locus_tag="IVW29_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7840388.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1037..2041
                     /gene="neuB"
                     /locus_tag="IVW29_002955"
     CDS             1037..2041
                     /gene="neuB"
                     /locus_tag="IVW29_002955"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7840389.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2038..>2571
                     /locus_tag="IVW29_002956"
     CDS             2038..>2571
                     /locus_tag="IVW29_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7840390.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          939 a          333 c          517 g          782 t
ORIGIN      
        1 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
       61 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      121 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      181 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      241 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      301 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      361 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      421 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      481 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      541 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      601 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      661 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      721 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      781 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      841 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
      901 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
      961 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1021 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1081 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1141 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1201 aaaatatcag aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt
     1261 agaattatct caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga
     1321 ttttttgtct actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca
     1381 aggatttaaa attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat
     1441 gtatgggttg ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc
     1501 tgtagattgt ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc
     1561 tagttctgaa gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc
     1621 cgttgggtac tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg
     1681 tgctaaggta attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa
     1741 agcttcttta gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat
     1801 atttttaggt gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc
     1861 aagaaaaagc ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc
     1921 tcttacaata aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg
     1981 caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg
     2041 aaaaaaatac ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct
     2101 gagaatttac tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc
     2161 cataaatatg gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa
     2221 gttcaaagtt tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag
     2281 ataaatggtt tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg
     2341 gatagaggag aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca
     2401 catattcacg gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg
     2461 aaaatagcat ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg
     2521 ggggaagatt cttggagagt ttttcccgtc ggagctccta gaatagatac t
//
LOCUS       DACCVU010000216         2519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCVU010000216 DACCVU010000000
VERSION     DACCVU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.224"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..338
                     /locus_tag="IVW29_002957"
     CDS             <1..338
                     /locus_tag="IVW29_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7840391.1"
                     /translation="ADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPII
                     LADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKK
                     VNKMNLEVE"
     gene            338..1714
                     /locus_tag="IVW29_002958"
     CDS             338..1714
                     /locus_tag="IVW29_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7840392.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2000..>2519)
                     /locus_tag="IVW29_002959"
     CDS             complement(2000..>2519)
                     /locus_tag="IVW29_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP7840393.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          932 a          352 c          457 g          778 t
ORIGIN      
        1 cagcagatga aagtatcttg tttgaactag gtttagataa aaaacaaata aaaagaaatg
       61 ttatgaaatt atctggtgga cagcaacaaa gagtagctat tgctagagca ttggtatcag
      121 atgctccaat aatactagct gatgagccta ctggtaatct agacagtgtt actgctggag
      181 aaataattaa tatattaaag acattagcta aagatagaaa taaatgtgta atagttgtaa
      241 cacatagtaa ggaagtagca gattctgcgg atatcatttt agaactaagt ggtaaaaagt
      301 tgaaaaaagt aaataaaatg aatttggagg ttgaataatg ataaaaaatg catttgctta
      361 tgtaactaga aaaagtttaa aatcattaat tatcatatta gttattttat ctatgtcaac
      421 tttaagtctc attagtttat ctattaaaga tgctacggac agagcttcaa aagaaacatt
      481 tgctaatata acaaatagtt tttctatgga gataaataga caagtaaatc caggaacacc
      541 tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag atatctcaaa cagatagtat
      601 agactcctat gtaaaaagaa taaacagtgt tgcagattta gttgattatg atattattga
      661 aactcaagag actcttgcga atcaatcacc tgaaagagca aagaatttta aaagaacagt
      721 tatgttaact ggggttaacg actcaacaaa agaaacaaaa tttgtatcag aagcatataa
      781 attagtagaa ggaaagcatt tagaaaatga agataaaaat aaaatcttaa tgcataaaga
      841 tttagctgaa aaaaataacc ttaaagttgg agataagata aaaataaaat ctaatttatt
      901 tgatgctgat aatgagaaag gtgcagatga aacagtagaa gtagaaatta aaggtctatt
      961 cgatgggcat aatagcggtg tagtaagtgc agcacaagaa ctatacgaaa atacattaat
     1021 cactgatatt catacagctg ctaaagttta tggtaatacc gaagatacag ctgtatacca
     1081 agatgcaaca ttctttgtaa aaggtgataa gaatcttgat agtgtaataa aagatcttgg
     1141 gaaattagat ataaattgga gagaatataa tttgattaaa agttcatcaa attaccctgc
     1201 attacaacaa tctatatcag gtatttactc aatttcaaat aaattatttt ttggctcatt
     1261 aatatttgca ggagttgtag tttcattatt attattctta tggatgaatg ctagaaagaa
     1321 agaaatagca gtattactat cattaggtat atcaaaatta gaaatttttg gtcaattcct
     1381 aattgagatg gtatttatat caatccccgc attcgttggt tcctatttct tagctcaata
     1441 tacagctgac aagcttggga ataatatatt gaataaggtt actggtgata tagctaaaca
     1501 aatagctaaa caatctgcat ctagccaatt aggtggtggg gcagaagctg aaggatttaa
     1561 caagacatta tcaagtttag atatcaatgt attacctaaa ttcatgattt atgtagttat
     1621 atttatgagt ttagtacttc ttgtatcatt gattatttct tcattcaata tattaagaag
     1681 gaatccaaaa gaattattaa ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa
     1741 aatgagagtg taatataaac cgtgtaagta accatcaatc ctaggcgggt ggttgcttca
     1801 cggtttttat ttttctaaaa taagatcaag aggtgattac acgatggcta gaaagaaaag
     1861 aaatcctgat gctgagaagt tagctgaatc cattttgaat acttatcaac ccgaatctgt
     1921 agaggatatg caagatgttt taaaagatgt gtttggtccc ttatttgaaa aaatgcttca
     1981 aggagaattg aataaccatt tagggtatga cgcccattct aaagagccaa agaaacatga
     2041 caatcgacga aacggttatg gcaataagac gcttaaaacg agctttggta aagtaaacat
     2101 tgatgttccc agagaccggg aggcttcttt tgaacctgag ttgattccta atagaaaacg
     2161 agatgtctcc gacatcgcag actgtagaca aaagctgttc caaactcgta actcgagctt
     2221 ggaacagctt tttatctact tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc
     2281 ctttccatag ccaggtggct aatttcttta aattcattgc agcaaacact agcatcgtat
     2341 gcgtcgcgac tttttccaag cctcgatact tggtccaacg catcccatgc ttttcttttg
     2401 catccgcaaa tattcgttca atcgtttgtt tccgtttttt gtaaattgat ttattcagat
     2461 ctgttaggcg aagatgttcc acttcatcca tcagatctcc ccaaacatgt ctcgtgatg
//
LOCUS       DACCVU010000217         2510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCVU010000217 DACCVU010000000
VERSION     DACCVU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.225"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..411
                     /locus_tag="IVW29_002960"
     CDS             <1..411
                     /locus_tag="IVW29_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840394.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            521..571
                     /locus_tag="IVW29_002961"
                     /pseudo
     CDS             521..571
                     /locus_tag="IVW29_002961"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            605..1981
                     /gene="tet(L)"
                     /locus_tag="IVW29_002962"
     CDS             605..1981
                     /gene="tet(L)"
                     /locus_tag="IVW29_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7840395.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
BASE COUNT          741 a          391 c          503 g          875 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgaaat gaatgtaaca gggttcaatt
      541 aaaagaggga agcgtatcat taaccctata aactacgtct gccctcatta ttggagggtg
      601 aaatgtgaat acatcctatt cacaatcgaa tttacgacac aaccaaattt taatttggct
      661 ttgcatttta tcttttttta gcgtattaaa tgaaatggtt ttgaacgtct cattacctga
      721 tattgcaaat gattttaata aaccacctgc gagtacaaac tgggtgaaca cagcctttat
      781 gttaaccttt tccattggaa cagctgtata tggaaagcta tctgatcaat taggcatcaa
      841 aaggttactc ctatttggaa ttataataaa ttgtttcggg tcggtaattg ggtttgttgg
      901 ccattctttc ttttccttac ttattatggc tcgttttatt caaggggctg gtgcagctgc
      961 atttccagca ctcgtaatgg ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa
     1021 agcatttggt cttattggat cgatagtagc catgggagaa ggagtcggtc cagcgattgg
     1081 tggaatgata gcccattata ttcattggtc ctatcttcta ctcattccta tgataacaat
     1141 tatcactgtt ccgtttctta tgaaattatt aaagaaagaa gtaaggataa aaggtcattt
     1201 tgatatcaaa ggaattatac taatgtctgt aggcattgta ttttttatgt tgtttacaac
     1261 atcatatagc atttcttttc ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca
     1321 tatcaggaaa gtaacagatc cttttgttga tcccggatta gggaaaaata taccttttat
     1381 gattggagtt ctttgtgggg gaattatatt tggaacagta gcagggtttg tctctatggt
     1441 tccttatatg atgaaagatg ttcaccagct aagtactgcc gaaatcggaa gtgtaattat
     1501 tttccctgga acaatgagtg tcattatttt cggctacatt ggtgggatac ttgttgatag
     1561 aagaggtcct ttatacgtgt taaacatcgg agttacattt ctttctgtta gctttttaac
     1621 tgcttccttt cttttagaaa caacatcatg gttcatgaca attataatcg tatttgtttt
     1681 aggtgggctt tcgttcacca aaacagttat atcaacaatt gtttcaagta gcttgaaaca
     1741 gcaggaagct ggtgctggaa tgagtttgct taactttacc agctttttat cagagggaac
     1801 aggtattgca attgtaggtg gtttattatc cataccctta cttgatcaaa ggttgttacc
     1861 tatggaagtt gatcagtcaa cttatctgta tagtaatttg ttattacttt tttcaggaat
     1921 cattgtcatt agttggctgg ttaccttgaa tgtatataaa cattctcaaa gggatttcta
     1981 aatcgttaag ggatcaactt tgggagagag ttcaaaattg atcctttttt tataacagga
     2041 attcaaatct ttttgttcca ttaaagggcg cgattgctga ataaaagata cgagagacct
     2101 ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac cgaaagacaa
     2161 taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg gacaaaataa
     2221 aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa aatactacct
     2281 gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg aggtggcaga
     2341 gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg
     2401 gttttggtcg gcactgccga cagcctcgca gagcccacct ttacgaagta aagtatagtg
     2461 tgttatactt tacatggaag ttgtaccgaa atattgtgtt gtgaagtcta
//
LOCUS       DACCVU010000218         2503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCVU010000218 DACCVU010000000
VERSION     DACCVU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.226"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            326..1372
                     /locus_tag="IVW29_002963"
     CDS             326..1372
                     /locus_tag="IVW29_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7840396.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1721..2353
                     /locus_tag="IVW29_002964"
     CDS             1721..2353
                     /locus_tag="IVW29_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7840397.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          936 a          357 c          499 g          711 t
ORIGIN      
        1 aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg aaatgagtaa
       61 ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg aaagtagtac
      121 gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga cttcaaatgc
      181 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      241 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      301 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      361 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      421 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      481 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      541 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      601 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      661 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      721 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      781 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
      841 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
      901 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
      961 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1021 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1081 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1141 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1201 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1261 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1321 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1381 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1441 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1501 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1561 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1621 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1681 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1741 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1801 ctgtgggaat gaaacgattg tatatcgatc aaacttacta acaggtagaa ccaaaagttg
     1861 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     1921 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     1981 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2041 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2101 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2161 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2221 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2281 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2341 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2401 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2461 tttgattgac aaatatcgag gtaatcatta attattgtgg ata
//
LOCUS       DACCVU010000219         2459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCVU010000219 DACCVU010000000
VERSION     DACCVU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.227"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..1150
                     /locus_tag="IVW29_002965"
     CDS             5..1150
                     /locus_tag="IVW29_002965"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7840398.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSKPKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVW29_002966"
     CDS             1321..1779
                     /locus_tag="IVW29_002966"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7840399.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVW29_002967"
     CDS             1938..2408
                     /locus_tag="IVW29_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7840400.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          824 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DACCVU010000220         2459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCVU010000220 DACCVU010000000
VERSION     DACCVU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.228"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..486
                     /locus_tag="IVW29_002968"
     CDS             <1..486
                     /locus_tag="IVW29_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7840401.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(693..1094)
                     /locus_tag="IVW29_002969"
     CDS             complement(693..1094)
                     /locus_tag="IVW29_002969"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840402.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1215..1298)
                     /locus_tag="IVW29_002970"
                     /pseudo
     CDS             complement(1215..1298)
                     /locus_tag="IVW29_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1321..>2459
                     /locus_tag="IVW29_002971"
     CDS             1321..>2459
                     /locus_tag="IVW29_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7840403.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGF"
BASE COUNT          779 a          497 c          496 g          687 t
ORIGIN      
        1 ttaaaggaag cgatcgatca aatcaagacg gctgtcttgt atcctcataa cggattaccg
       61 attttattga tgggtgtaag cggttcagga aaaacctttt tggcagaaaa aatttatgaa
      121 tacgccaagg aacaacaggt gatagaatca aatgctccat ttatcgccta taattgtgct
      181 caatacttta atttctcgat taacaagatc gtagaaaatt taacgatcct tgatgcaatg
      241 aaggttttga gattggtaca aaaggccgtc aaagaactcg aaaaactaat ggggatcgaa
      301 ttttcaaaca atcaattgtt cttgctttac atgcactgct gctcaatgat cgaacgtatt
      361 ttacgcaaag aaagtgtaga tgaacaagca gatatcaaag aatatattca aaaagaggga
      421 cataattatg aaatatcaaa atacccggct tacaaaacga agtttgaaag tactgtaata
      481 aagtaagatt taaataagca taaacaaggt aagaaccata ggggctgtgt caagtccaaa
      541 atcttgtgta aaaaggaaat ctcaaagtac gatgttttgc cttgttgagc ctagctacgg
      601 tccaatatgc tggtaagaag tgtcaagagc aaaaagcgaa gcgtctcttg atacttctca
      661 ccagtatatt atactattgg cggcttaatg tgctaaatct cgttacgatc aaattcaaga
      721 tattttctag gtgcagaagt ccattcgtca tccttttcca tcaataaagc accgatgata
      781 cgaataacag aatcacaatt tgggaaaatt cggacaacac gttcacgacg gcgtacttct
      841 tcatttagtc tttcaagaac attcgttgtt ctcaatcgtt gacggatatt ttctggtaaa
      901 accattactt gcatggcatc ttcgaagccc tcatccaata ttttacaggc agttgcgact
      961 ttcttgtctt ccccatattt atccataata agttgtttac gttctccagc cagctcgatg
     1021 cttgccgttt tgaagatggc tttaaagtct tctttgatat cgcttgagat tttcttggag
     1081 aatttgtcgg aaatgttgcg aaggagatgt gcttggcatc tctgctagct cgcacaaaga
     1141 aggtattctt tcacagcttt tgtgagccct atatgcgcat ccgagatgac catctttact
     1201 cctgaaagac cgcgttcttt caatccgaga gtgtaaaata ttttgtgtaa atagaaaaaa
     1261 ggaagtccct tcagtagaat agagttacca caacacattc acagaaaaga ggacttccat
     1321 atgaacgatt ttactacaga aattctaaag actctagcga ataaaggcga tttgaatgaa
     1381 ttattccgtg tccatttgga aaaagctgtc aatacgcttc tcaaaacgga gttaacggct
     1441 ttcctcgatt acgaaaagta cgatcgcatt ggttttaaca cgggtaattc tcgtaacggc
     1501 tcctatgacc gtacggtcaa gaccgagtac ggggaacttc atctccagat tccgcgcgac
     1561 cgcaacggcg agttcaagca acagactgtt cctgcttata gacggacgaa tgatacctta
     1621 gaggagaccg tcattcacct cttccgaaaa ggtattacca tgtcggaaat cgcagacttg
     1681 attgagaaaa tgtatgggca tcactacacg ccccaaacca tgtccaatat gactaaatca
     1741 tttacggaag aagtcacttc atttaaggaa cgtgagcttc atgaccgtta tgccgctatt
     1801 tatatggacg caacctatat ccctttaaag cggaaaaccg tcgccaagga agccattcat
     1861 atcgcagttg gcattcgccc ggacggttca aaggaagtgt tgagctatgc gattgcacca
     1921 actgaatcta tcacgatttg ggaagagatt ttaatagacc tccaggaacg tggcgtgaaa
     1981 aacgtcctcc ttttcatcac ggatggctta aaggggatgg caggggccgt gcagcggttc
     2041 taccccaaag cccgttttca acattgctgt gtgcacgttt cccgtaatat cagtcacaaa
     2101 gtgcgtgtcg atgatcgtaa ggaagtctgt gatgatttta aaatggtgta tcaagcgtca
     2161 tctaaaaagg cggcattgga agcacgtggt gcttttgcgg aaaaatggaa aaccagttat
     2221 ccaaaaatgg tggaatcgat cctttcgaac gattacctgc tcactttcta tgattttcca
     2281 ttagccatac gcaagactat ttactctacg aacttgattg aatcctttaa taagcaaatc
     2341 aagaaataca gccacagaaa agagcagttt caaaatgaag aatcactaga gcgtttccta
     2401 gtatccatct ttgatacata caatcaaaaa ttcttaaata gaagccataa aggcttcca
//
LOCUS       DACCVU010000221         2405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCVU010000221 DACCVU010000000
VERSION     DACCVU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.229"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..103)
                     /locus_tag="IVW29_002972"
     CDS             complement(<1..103)
                     /locus_tag="IVW29_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7840404.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IVW29_002973"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IVW29_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IVW29_002974"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IVW29_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DACCVU010000222         2403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCVU010000222 DACCVU010000000
VERSION     DACCVU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.230"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            103..2256
                     /locus_tag="IVW29_002975"
     CDS             103..2256
                     /locus_tag="IVW29_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7840405.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          821 a          424 c          534 g          624 t
ORIGIN      
        1 gaaattaggt aaagaatttc ttgctccgcc cattttttct tttttattaa gaagtttttg
       61 ggtacaatga agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg
      121 aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag
      181 aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata
      241 tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa
      301 gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc
      361 ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc
      421 acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg
      481 aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt
      541 tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag
      601 caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt
      661 aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat
      721 gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg
      781 gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat
      841 aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt
      901 ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga
      961 caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa
     1021 gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa
     1081 ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg
     1141 aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat
     1201 gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa
     1261 caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca
     1321 gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga
     1381 aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc
     1441 tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat
     1501 acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga
     1561 ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag
     1621 tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt
     1681 tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt
     1741 aactttaccg atttggcatt tggacagttt gacacatata caccaatcca attagctcaa
     1801 tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc
     1861 tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta
     1921 gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa
     1981 gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac
     2041 ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca
     2101 gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt
     2161 gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata
     2221 atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct
     2281 tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt
     2341 gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca
     2401 aaa
//
LOCUS       DACCVU010000223         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCVU010000223 DACCVU010000000
VERSION     DACCVU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.231"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVW29_002976"
     CDS             207..2069
                     /locus_tag="IVW29_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7840406.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IVW29_002977"
     CDS             2069..>2400
                     /locus_tag="IVW29_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7840407.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACCVU010000224         2375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCVU010000224 DACCVU010000000
VERSION     DACCVU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.232"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..586
                     /locus_tag="IVW29_002978"
     CDS             <1..586
                     /locus_tag="IVW29_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7840408.1"
                     /translation="IYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTD
                     YRNNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYIL
                     RYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMY
                     EKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            587..1339
                     /locus_tag="IVW29_002979"
     CDS             587..1339
                     /locus_tag="IVW29_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7840409.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1344..2291
                     /locus_tag="IVW29_002980"
     CDS             1344..2291
                     /locus_tag="IVW29_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7840410.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          837 a          306 c          459 g          773 t
ORIGIN      
        1 tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat
       61 tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag
      121 aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga
      181 attagactgt atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt
      241 aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata
      301 tatattgaga tacgatgata aaattataga aattcattta gattattttg gaagagttcc
      361 tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact tattaaataa
      421 taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta
      481 tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga
      541 tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt
      601 tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc
      661 aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc
      721 tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac
      781 taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt
      841 agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt
      901 tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga
      961 attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag ttgtttctgc
     1021 aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa
     1081 taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga atgcctcaat
     1141 gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg
     1201 gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag attttaaatg
     1261 gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa
     1321 ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca
     1381 ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact
     1441 tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag
     1501 atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct
     1561 ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc
     1621 gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt
     1681 taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa
     1741 aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag
     1801 aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt
     1861 tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc
     1921 tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg
     1981 atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag
     2041 ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca
     2101 cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat
     2161 acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact aagatccagt
     2221 caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg
     2281 aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa
     2341 ggactgatga gttttgtaaa atcaaatctg ggata
//
LOCUS       DACCVU010000225         2354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCVU010000225 DACCVU010000000
VERSION     DACCVU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.233"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(118..189)
                     /locus_tag="IVW29_002981"
     tRNA            complement(118..189)
                     /locus_tag="IVW29_002981"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Glu,seq:ttc)
     gene            complement(201..274)
                     /locus_tag="IVW29_002982"
     tRNA            complement(201..274)
                     /locus_tag="IVW29_002982"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Ile,seq:gat)
     gene            complement(287..357)
                     /locus_tag="IVW29_002983"
     tRNA            complement(287..357)
                     /locus_tag="IVW29_002983"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Gly,seq:tcc)
     gene            complement(362..434)
                     /locus_tag="IVW29_002984"
     tRNA            complement(362..434)
                     /locus_tag="IVW29_002984"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Phe,seq:gaa)
     gene            complement(438..510)
                     /locus_tag="IVW29_002985"
     tRNA            complement(438..510)
                     /locus_tag="IVW29_002985"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Asp,seq:gtc)
     gene            complement(529..602)
                     /locus_tag="IVW29_002986"
     tRNA            complement(529..602)
                     /locus_tag="IVW29_002986"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(566..568),aa:Met,seq:cat)
     gene            complement(615..704)
                     /locus_tag="IVW29_002987"
     tRNA            complement(615..704)
                     /locus_tag="IVW29_002987"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(666..668),aa:Ser,seq:tga)
     gene            complement(727..798)
                     /locus_tag="IVW29_002988"
     tRNA            complement(727..798)
                     /locus_tag="IVW29_002988"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Glu,seq:ttc)
     gene            complement(820..907)
                     /locus_tag="IVW29_002989"
     tRNA            complement(820..907)
                     /locus_tag="IVW29_002989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(871..873),aa:Ser,seq:gct)
     gene            complement(932..1005)
                     /locus_tag="IVW29_002990"
     tRNA            complement(932..1005)
                     /locus_tag="IVW29_002990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(969..971),aa:Ile,seq:gat)
     gene            complement(1019..1089)
                     /locus_tag="IVW29_002991"
     tRNA            complement(1019..1089)
                     /locus_tag="IVW29_002991"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Gly,seq:tcc)
     gene            complement(1091..1166)
                     /locus_tag="IVW29_002992"
     tRNA            complement(1091..1166)
                     /locus_tag="IVW29_002992"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Asp,seq:gtc)
     gene            complement(1185..1258)
                     /locus_tag="IVW29_002993"
     tRNA            complement(1185..1258)
                     /locus_tag="IVW29_002993"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1222..1224),aa:Met,seq:cat)
     gene            complement(1271..1360)
                     /locus_tag="IVW29_002994"
     tRNA            complement(1271..1360)
                     /locus_tag="IVW29_002994"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Ser,seq:tga)
     gene            complement(1384..1457)
                     /locus_tag="IVW29_002995"
     tRNA            complement(1384..1457)
                     /locus_tag="IVW29_002995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Ile,seq:cat)
     gene            complement(1471..1544)
                     /locus_tag="IVW29_002996"
     tRNA            complement(1471..1544)
                     /locus_tag="IVW29_002996"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1508..1510),aa:Met,seq:cat)
     gene            complement(1572..1644)
                     /locus_tag="IVW29_002997"
     tRNA            complement(1572..1644)
                     /locus_tag="IVW29_002997"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1609..1611),aa:Ala,seq:tgc)
     gene            complement(1664..1737)
                     /locus_tag="IVW29_002998"
     tRNA            complement(1664..1737)
                     /locus_tag="IVW29_002998"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1701..1703),aa:Pro,seq:tgg)
     gene            complement(1747..1820)
                     /locus_tag="IVW29_002999"
     tRNA            complement(1747..1820)
                     /locus_tag="IVW29_002999"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1784..1786),aa:Arg,seq:acg)
     gene            complement(1831..1916)
                     /locus_tag="IVW29_003000"
     tRNA            complement(1831..1916)
                     /locus_tag="IVW29_003000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1880..1882),aa:Leu,seq:taa)
     gene            complement(1933..2004)
                     /locus_tag="IVW29_003001"
     tRNA            complement(1933..2004)
                     /locus_tag="IVW29_003001"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1970..1972),aa:Gly,seq:gcc)
     gene            complement(2016..2088)
                     /locus_tag="IVW29_003002"
     tRNA            complement(2016..2088)
                     /locus_tag="IVW29_003002"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2053..2055),aa:Thr,seq:tgt)
     gene            complement(2106..2187)
                     /locus_tag="IVW29_003003"
     tRNA            complement(2106..2187)
                     /locus_tag="IVW29_003003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Leu,seq:tag)
     gene            complement(2195..2267)
                     /locus_tag="IVW29_003004"
     tRNA            complement(2195..2267)
                     /locus_tag="IVW29_003004"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Lys,seq:ttt)
     gene            complement(2271..2343)
                     /locus_tag="IVW29_003005"
     tRNA            complement(2271..2343)
                     /locus_tag="IVW29_003005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2308..2310),aa:Val,seq:tac)
BASE COUNT          647 a          646 c          546 g          515 t
ORIGIN      
        1 ttacattaga actgtccaaa aaagcttagc acaatcatcc ataaagataa ctctgctaag
       61 ctttttaaaa tcattctttc agataaaaag aaaaagatac tcaaaaagta tcttccatga
      121 cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
      181 caacgggcca ataaaataaa tgggcctaaa tggactcgaa ccatcgacct cacgcttatc
      241 aggcgtgcgc tctaaccagc tgagctatag gcccataaaa actaaaagcg ggtgacgaga
      301 atcgaactcg cgacaacagc ttggaaggct gtggttttac cactaaacta cacccgcata
      361 atggcgcggg acagaatcga actgccgaca catggagctt caatccattg ctctaccaac
      421 tgagctaccg agccaaacgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg
      481 caggcatgtt aacccctaca ccacggaacc aatgttttta gtttttaatt gcgggagcag
      541 gatttgaacc tacgaccttc gggttatgag cccgacgagc taccagactg ctccatcccg
      601 cgataatatg aaaaaggagg ataagggatt cgaacccttg cacggtttta cccgcctgac
      661 ggttttcaag accgttccct tcagccggac ttgggtaatc ctccatgtaa aactatatta
      721 aattaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa
      781 ccgcttgacc aacgggccag gtaaaacaaa atattgaaac ggagaaggag ggatttgaac
      841 cctcgcgccg gtttcccgac ctacaccctt agcaggggcg cctcttcagc cacttgagta
      901 cttccccaaa accaataaga ttttgtacta atgggcctaa atggactcga accatcgacc
      961 tcacgcttat caggcgtgcg ctctaaccag ctgagctata ggcccataaa aaactaaaag
     1021 cgggtgacga gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac
     1081 tacacccgca tggcggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
     1141 catgttaacc cctacaccac ggaaccagtg tttttagttt ttaattgcgg gagcaggatt
     1201 tgaacctacg accttcgggt tatgagcccg acgagctacc agactgctcc atcccgcgat
     1261 aatatgaaaa aggaggataa gggattcgaa cccttgcacg gttttacccg cctgacggtt
     1321 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc atatgaaact tacaacgcca
     1381 caatggacct tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctaacc
     1441 aactgagcta aaggtccagg ctcttaaaaa tcgcggcgga ggggatcgaa cccccgacct
     1501 cccgggtatg aaccggacgc tctagccagc tgagctacac cgcgaaaaag atattcatta
     1561 tttaaatgaa atggagccta gcgggatcga accgctgacc tcctgcgtgc aaagcaggcg
     1621 ctctcccagc tgagctaagg ccccgtgttt aaatttataa ttatcgggaa gacaggattc
     1681 gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta cttcccgttt
     1741 aattaacgcg cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta
     1801 tccagttgag ctatgggcgc atatttgaaa tgccgaggac cggaatcgaa ccggtacggt
     1861 gatcactcac cgcaggattt taagtcctgt gcgtctgcca gttccgccac cccggcgcga
     1921 ttgctttggc aaagcggaaa acggggttcg aacccgcgac ccccaccttg gcaaggtggt
     1981 gctctaccac tgagctattt ccgcgtgttt aaagatgccg gctaaaggac ttgaaccctc
     2041 gaccctctga ttacaaatca gatgctctac caactgagct aagccggcat gtaaaactta
     2101 aattatgcgg gtgaagggac ttgaaccccc acgccgtaag gcgctagatc ctaaatctag
     2161 tgcgtctgcc aattccgcca cacccgcaaa aatatgagcc gtacagggct cgaacctgtg
     2221 accctctgat taaaagtcag atgctctacc aactgagcta acggctcata tggaggttaa
     2281 cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc
     2341 tccatggata actt
//
LOCUS       DACCVU010000226         2270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCVU010000226 DACCVU010000000
VERSION     DACCVU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.234"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            191..445
                     /locus_tag="IVW29_003006"
     CDS             191..445
                     /locus_tag="IVW29_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7840411.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IVW29_003007"
     CDS             446..763
                     /locus_tag="IVW29_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7840412.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="IVW29_003008"
     CDS             1322..2257
                     /locus_tag="IVW29_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840413.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DACCVU010000227         2243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCVU010000227 DACCVU010000000
VERSION     DACCVU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.235"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            80..1159
                     /locus_tag="IVW29_003009"
     CDS             80..1159
                     /locus_tag="IVW29_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7840414.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            1461..2009
                     /gene="aac(6')"
                     /locus_tag="IVW29_003010"
     CDS             1461..2009
                     /gene="aac(6')"
                     /locus_tag="IVW29_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7840415.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          683 a          493 c          567 g          500 t
ORIGIN      
        1 ttggaaataa aaaaacgcca cgtgaattcc atgttatatt gaagttacca caactctcaa
       61 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
      121 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      181 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      241 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      301 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      361 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      421 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      481 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      541 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      601 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
      661 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
      721 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
      781 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
      841 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
      901 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
      961 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
     1021 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
     1081 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
     1141 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
     1201 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
     1261 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat
     1321 gggtggctaa cttaaacttg aaatttacga taaataatga aagaatagag atactgattg
     1381 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
     1441 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
     1501 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
     1561 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
     1621 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
     1681 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
     1741 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
     1801 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
     1861 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
     1921 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
     1981 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
     2041 tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt
     2101 atttccgaag aagctacttt tggaacaccg tgtaccagag gttgtgaaaa agtcgcttag
     2161 tttcgagtag taagaaaaat tttgtgaaaa tggctttcta catttttgca taaatttggc
     2221 ttaataccga gaaactggct ttt
//
LOCUS       DACCVU010000228         2094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCVU010000228 DACCVU010000000
VERSION     DACCVU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.236"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(248..469)
                     /locus_tag="IVW29_003011"
     CDS             complement(248..469)
                     /locus_tag="IVW29_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840416.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="IVW29_003012"
     CDS             complement(567..812)
                     /locus_tag="IVW29_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840417.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="IVW29_003013"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="IVW29_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          709 a          362 c          303 g          720 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctt
//
LOCUS       DACCVU010000229         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCVU010000229 DACCVU010000000
VERSION     DACCVU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.237"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            194..1420
                     /locus_tag="IVW29_003014"
     CDS             194..1420
                     /locus_tag="IVW29_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7840418.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1528..1998)
                     /locus_tag="IVW29_003015"
     CDS             complement(1528..1998)
                     /locus_tag="IVW29_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7840419.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          759 a          321 c          405 g          580 t
ORIGIN      
        1 cgaactgcgt gtattcggtc tttcgatctt tttcacggta tggtatgata gttaagaatt
       61 ttgagaaatt ttaaaaaata ataatcgtta cacaaaaatg ttttttaaag ttttcttatg
      121 attaaaaagt gaatttgctg attgaacagt tatatgagac aatattttag aaaatgtgaa
      181 acagaggtgt attatgaagc gatggcaaaa agttctatta accttgttag gggtattcgt
      241 tttgctgatt gccggattat cagcatacgg aatcagattt atgggagaag cgagccaaac
      301 agtaaacaaa atttctaaaa actctaatcg aatatcctcc aaacgttctg agagagtaag
      361 cattgatgac aaagaacctt tttcggtttt gctattaggt ttagataccg gtggattagg
      421 aagaacggaa caaggacgtt ctgatacgat gatggtagta acagttaatc cgaaacaaaa
      481 aaaatcaacg atcatcagtt tggaccgaga tatttatacg aatatcgtag gttatggaac
      541 ggtagataaa ttaaatcatg cctacgcatt tggtggtgtc gaaatggcca tggattcaat
      601 tgagcaactg ctcgatattc ctattgatca ttatgtaacg atcaatttgg atggcatgga
      661 agatttgatc aatgcggtcg gtggcatcaa agtgaataat aaaattgact ttactttgga
      721 tggtgtccat gtaccagtag gtgaacaaac attgaatgga gaaaaaggtt tagcctattc
      781 tcgaatgcga cacgaagatc cagaaggcga tatcggtcga caacgtcgtc agcgggaagt
      841 agtgacgaaa atcgtcaata aagttttaag tttagatggc gtaagcaatt atagaaaaat
      901 tttaaaagca gttgagaaca atgtgacgac ggacttagat tgggatgaca tgcttgatgt
      961 agcgacgaac tacaccccgg cgtttgaaac aatcaaacaa gaccagttgc aaggcgaagg
     1021 acaaatgatc aatgatatct actatcaaat tttaggaaaa aatgatcttc tttcgatcca
     1081 aaatgaactg aagaaacaat taaatataaa aacaagcgat acattaccta atttgaaaaa
     1141 tgagaatgct tcaatgatgt tctttgatga ttcggaagat ggcgattcga caaacggtga
     1201 tggatcgaat agtgaagggt acaccgatta ttcttcctat tcagaaaaca catatgatca
     1261 gtaccaagat ccgaatacct attatcagca aacagaagat acaagtcagt actatacacc
     1321 aagtcaagat actcaaagtt atgatcctta cgctggacag gaacaaaatg gttatggtta
     1381 tgaccagaat aatgagtatg gaacaacaag tgggtactag aaaaaaatag tttggttaat
     1441 aaagagtgcc acctaaatta gacaaaaaat ctaacttagg tggcactttt tctatgtgtt
     1501 aaattacagg acgtctcttt ttagcgctta gtttaaaaaa taaattgaat aattcaatag
     1561 agttgcgaat agtacccata aataataagg aatcatgagc cacattgcca ggcgatgatg
     1621 cagccacaac tgatacataa gcactgacaa gaaaataact aaaagtgtaa tatcgattgc
     1681 tgcaatgaaa atatttctta aattaaaaaa tagagcagac catataaaat taaaaatgaa
     1741 ctgaatcaca aaaagaccga caagattgtt tcgctgtttt ttgttgttgt gattaagtaa
     1801 caaataaaga gaaataccca tcaatatata cagtaaggtc cacatcggcc caaatatcca
     1861 gctaggtggt gcaaatggtg gaagttgcaa agaatagtaa tattttccgg gatttccgct
     1921 aagcaaccct gataaaaagc ctaatatgac aattcctata acacaaaccc aaaaacgaat
     1981 atcttttatc atcttcattt tactaactcc tttctataat attagtttac catgaaggct
     2041 cacagcaaga cctgtattta gtttg
//
LOCUS       DACCVU010000230         2055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCVU010000230 DACCVU010000000
VERSION     DACCVU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.238"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..189
                     /locus_tag="IVW29_003016"
     CDS             <1..189
                     /locus_tag="IVW29_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7840420.1"
                     /translation="QFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQK
                     KQSEPVPHEVKGTGSDLL"
     gene            371..1555
                     /locus_tag="IVW29_003017"
     CDS             371..1555
                     /locus_tag="IVW29_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7840421.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            1552..1842
                     /locus_tag="IVW29_003018"
     CDS             1552..1842
                     /locus_tag="IVW29_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840422.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            1839..>2055
                     /locus_tag="IVW29_003019"
     CDS             1839..>2055
                     /locus_tag="IVW29_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840423.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIG"
BASE COUNT          682 a          351 c          426 g          596 t
ORIGIN      
        1 caatttttct taaaacccat caagggaaga ggttacgtgg atacaggaaa aagtgtgatt
       61 agtgagtttt atacacccct tgtaccaaaa aggtacgatt tcttaggaga aataggaaaa
      121 gtcattcaaa aaaagcaatc tgagcctgta ccgcacgaag tgaaaggtac aggttcagac
      181 ttgctttgat tggtgtggcg ttagccacgc caatccttta gccccctcta tctaacaggg
      241 gggcacaaat acaactgaaa acaggcaatt ctcatcaacc aatgttgatg gtacaaagga
      301 ttgcggaaaa tatgaaaggt gtaaactcaa gggtcatagt ttacatcttt tttagattgg
      361 aggtttgcga atggaaaacg aaaaaatttg gattaaggaa ttgaaagaaa agcgactggc
      421 ttatggcgtt tctcaaaata aactggcggt tgcttctcat attaccagac cttacctatc
      481 ggatattgaa acaggcaagg cagtccctac tcaacaggtg aaagaagatt tactgaacgc
      541 tttggaacgc ttcaatcccg ataacccatt ggagatgtta ttcgactatg tacggattcg
      601 attccctacc aatgatgtgg ctcaaattat cggtgaggtt ttacgtttga atatggatta
      661 tatgctacac gaagactttg gctattattc ttatcccgaa cactatcgtt tcggtgatat
      721 tgttgtccta gtctctcatg atgtaagtaa aggtgtttta ttagaactga aaggaaaagg
      781 ctgtcggcaa tttgaaaact ttctattggc tcaacatcgg agctggtatg atttctttca
      841 agattgcatg gaacataaag gtattttcaa acgcttggat ttagctatca acgataaaac
      901 agggattttg aatattccag atttggcaaa gaaatgtaaa caggaagaat gcatttccgt
      961 ctttcgaagc tttaaaaatt atcgcagtgg tgaattggtt caccgtgatg aaaaaccata
     1021 catgggaaac acactttata ttggctcatt aaaaagtgaa gtctatttct gtatatatga
     1081 gaaagattat gagcaatacg tgaaaaatga tgttccctta gaagacgcag aagtaaagaa
     1141 tcggtttgaa attcggttaa aaaatgaccg agccacccaa gccatgaaag atttattggc
     1201 acatcaacag gcggaaaaaa cagcctttga aattatcaat cgctatatcc gctttgcaga
     1261 taaagacgac actaaacgtc ggagtgattg gaaaactaat gaacgttggg aatggtttat
     1321 cggtaaaaat cgtggagcat tacgcctaac cacacagcca gagccttatt cttttgaacg
     1381 aacattgaac tggttacatc atcaagtcgc ccctacatta aaaatcgcaa gcattttaga
     1441 tgttctcaac ggtacaacca ttatctccac catgattcag gaagccaaac tgacagaaaa
     1501 acatgagaaa ttaattgaac aacaacatct agccatggag gatttgatta catgagcctt
     1561 ttaacaaaag aattaaagaa gttagggttt cagtgtggca ttgagtttca agcgtatata
     1621 caaaatacag ggaaatatac gtctctcatt attgagggaa aacggcaagc tggtgatacc
     1681 atctatacct atgattttta caaggtgaga ttttataata attataccaa tcgagtaacc
     1741 gtctatggag agcatttaac cccttttcaa ttattaaggc gtgtaaaaag ctatatctat
     1801 tatcgagaaa aatatcttaa ggaaaggagg acaatcacat gaattttggg cagaatttgt
     1861 acaactggtt cttggataat gcacagtcat tggtattgtt agcgattgtt gtcattggac
     1921 tgtttttagg ttttaaacga gaattttcaa aactcattgg cttcttgatt attgctctaa
     1981 tcgccgtcgg acttgtgttc aatgcagctg gcgtgaaaga tgtgttatta aacttattta
     2041 accgaatcat tggtg
//
LOCUS       DACCVU010000231         2026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCVU010000231 DACCVU010000000
VERSION     DACCVU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.239"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..912)
                     /locus_tag="IVW29_003020"
     CDS             complement(<1..912)
                     /locus_tag="IVW29_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7840424.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IVW29_003021"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IVW29_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7840425.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          673 a          368 c          396 g          589 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttga
//
LOCUS       DACCVU010000232         2018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCVU010000232 DACCVU010000000
VERSION     DACCVU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.240"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1894
                     /gene="ltrA"
                     /locus_tag="IVW29_003022"
     CDS             <1..1894
                     /gene="ltrA"
                     /locus_tag="IVW29_003022"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7840426.1"
                     /translation="ETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMDIILSRE
                     NILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYRPKPVRR
                     KEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSVENAIKA
                     TYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKM
                     TNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPC
                     KSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEK
                     TRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKG
                     KTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTG
                     RQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHT
                     NLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIV
                     PTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGN
                     QELNLI"
BASE COUNT          800 a          323 c          388 g          507 t
ORIGIN      
        1 tgaaacattg tgtgtagaag atttaagaca cgctgaatat tacgaaatgc aaaacacctt
       61 tgatgattta tatgccaaaa gtaagaatgg agatattttc actcatctta tggatattat
      121 cctatcgaga gaaaatatat tactggcata tcgaaatatc aaagcaaatg caggcagtaa
      181 aacagcagga acagacggta caataattaa agatattggc aaattgccag cagaaacagt
      241 tgtcaaaaaa gtaagataca ttgttgcagg cagtccacat gggtatcgac ctaagccagt
      301 gaggcgaaaa gaaataccaa aaccaaatgg taaaaccaga cctctaggta ttccttgtat
      361 gtgggacagg cttatacaac aatgtataaa acaagtatta gaaccaatct gcgaagctaa
      421 atttagtgaa aacagctatg gttttagacc taataggtca gttgaaaatg caataaaagc
      481 aacctataac cgcctacaaa tcagccagct acattatgtg attgaatttg atattaaggg
      541 tttctttgac aacgtgaacc attccaagct gataaaacag atatgggcta tgggaatacg
      601 ggacaagcac ttgatattca ttttaaaaag gatattaaaa gctccaatca aaatgactaa
      661 cggtacaata acatatccag agaaaggaac tccgcaaggg ggaataatat ccccactact
      721 agcaaacatt gttcttaatg agttagacca ttgggtagaa agccaatggc aggaaaatcc
      781 tgttacaaaa aactatgtgg ttcatatcaa taaaagcggt agcccatgta aaagcaacgc
      841 ttacaaagaa atgaagaaaa caaagttaaa ggaaatgtat atggttcgtt atgcagatga
      901 ttttagagtt ttctgccgct acaaggaaag tgctgaaaaa gccaaaatag caatcactca
      961 atggattgaa caacgattaa aattggaagt atcacaagaa aaaacaagaa ttgtaaatgt
     1021 tagaaaaaga tactccgact ttcttggttt caagattaaa atgataccga gaagaaagaa
     1081 gttagttgtt aaatcacata taagtgataa acaattcaaa aaccaaaaag acaagctggt
     1141 atcacaggca aagaaaattg cttctccctc aaaagggaaa acggaattag aagaaacaag
     1201 actttataat tcaatggtat taggtatgca aaattattat cagattgcaa cgaatgtaaa
     1261 tctggattgt atgaaactga atagagctgt aatggctatc ttcactaaca gattaggtac
     1321 aaacaaaagg ggcagattga gaaaaacagg cagacaactt acgacaaagg aggcaacaag
     1381 atatggtaaa tctaaaatgc ttcggtatgt tgctggcgag ggcgaaccta ttttccctgt
     1441 tggatatatc caacacagaa aaccaatggc aaaggtgtat ttagcgaatt gctacactct
     1501 agagggaaga aaatttatcc acaccaatct aagtgttgat aaattactga tgtaccagtt
     1561 aatgaaatta tctttggaaa atcgcacaat agaatacgca gacaatcgaa tttcgctatt
     1621 ttctgctcaa catggaaaat gtgctataac tctggaagaa tttcaacaag ctaatgaaat
     1681 acattgtcat catattgtac caactggagc tggtggaaat gacgattata aaaatcttat
     1741 tttggtaaaa gaggcggtac accgtctaat acacgctaaa acagaagaaa caatacaaaa
     1801 atatatagct ctattgaagc taaataaagt ccagctcata agactaaata aatatagagt
     1861 attagctggc aatcaagaat taaacttgat ataggtctac tacataaata cttttcaata
     1921 taaacacgat ggaacgcgga gtgcggtgaa agtcgcacgc tccgtgtgaa tggggggaaa
     1981 agtttgagat atttataata tcaggaactt acctatcc
//
LOCUS       DACCVU010000233         2000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCVU010000233 DACCVU010000000
VERSION     DACCVU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.241"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..375
                     /locus_tag="IVW29_003023"
     CDS             <1..375
                     /locus_tag="IVW29_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7840427.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            423..1823
                     /gene="yfcC"
                     /locus_tag="IVW29_003024"
     CDS             423..1823
                     /gene="yfcC"
                     /locus_tag="IVW29_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7840428.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1856..>2000
                     /locus_tag="IVW29_003025"
     CDS             1856..>2000
                     /locus_tag="IVW29_003025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7840429.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          552 a          339 c          432 g          677 t
ORIGIN      
        1 aatgtttcca cagaggactt ctttaattat tgtcgattag aggaaagtgg tgtaacagtc
       61 actagcggta cagatttacc gctgtttatt actgatattc ctgaatcgat tataagagca
      121 gtattccgtc gttttcctga aggagaagaa gtctgggaga aagaaacaag cttcacgaca
      181 accgagctgc tgaaaagttt tacaagcaac agcttcaaag taaatggttt atcggagtac
      241 ggagaaataa aagtagggaa aagcgccagc ttagctgttt tcagcgagga cctctgtact
      301 gatgactggc aaaaagtgat ggctgcggaa gttatcgcta cctatcttga tggtgactta
      361 gtttacaaga aataatgctt ttcgatataa cgttataaaa cgaattaaag agaggttaaa
      421 aaatggaaac aatgaaaaga aaattcacct tcccacatag ttatacattg atttttatct
      481 taattatttt ggctgcaatt ttaacttgga taattcctag tggtcagttt gagacagttg
      541 ttgaaaacgt cgcaggtatg gaaaaaacaa cggttgttcc gggaacctat caccaaatcg
      601 ataaggcaga ttatggacaa ggtttttcct ctatactaat ggcaccagct gaagggatta
      661 tttcagctgt tgaggttgtt gcttttgttc tcatcgttgg tggtgccttc gggattatcc
      721 taaaaacggg agccatcgat cgagggctgt ttaccttagc aaaagttctg ggagacaagg
      781 ggatcttagt tattccgatg tcgatgattc tattcagctt aggaggatca acctttggca
      841 tgagcgagga agtcattccc ttatttgcgg tgtttatttc tctgatgttt tctcttggct
      901 atgattccat gactgcgatc ttgattctat ttttaagtac acaagcggga tatgttgggt
      961 cgacgattaa tcccttcaac gttttaatcg cacagggagt agctggcgtt tacggaaatc
     1021 ccctgctttg gtttcggatg ctatcctgga ttgtcattac tgggatttca attgttttca
     1081 ccatgcttta tgctagcaga gtcaaaaaga atccactgtc ctctattgtt tatgagagtg
     1141 atttaaaggt taagcataag tttcaaaatt ttgacactga aaaagagttt tcaacacaag
     1201 ataaactaat tatcgccggt tttatcgtag gtttaggaat catgatttgg ggtatcattt
     1261 ccaagggatg gtatatggtt gaaatttctg cactattctt aggattaggt ctttttgctg
     1321 ggggcatcgc tagattttct caaaaggaaa ttgctgaagc ttttgtcgaa ggctgttcag
     1381 atttcgccta tgctgctgta atcattgggc tagctagagg agtcttggta atcttggaag
     1441 gtggaatgat tattgacact attctaaaca gcttggctaa ccttttaggt ggtcttccaa
     1501 aattcctgtt cactacgatt ctactcattt ctcaagtaat tgtttccttt tttgttccat
     1561 cctcctcagg agctgcggca ttaacgatgc cggttatggc accattgggt gatctaatga
     1621 atgtaagtcg tgatgttgtt gttctaagta accagtttgg aaatggttta atgaatatta
     1681 tctccccaac tagtggtgta ttactggcag gattagccat cgcagagatt aatttctcta
     1741 aatggtttaa ggttggcttc aaaatatttg ccatcttaac ccttgtttct gctgttttgt
     1801 tatacattgc tacactcttt tagtcttaat catccattaa taggaggcaa tagttatgat
     1861 taaaataaat caattagcag gaatgaatat ccactattta ttttattctc tcaaagaatt
     1921 tttcgcttat caacagaaaa ttggtatttc ttctgttgaa ctttggggag gtgctccgca
     1981 ttttttcata gatgctttta
//
LOCUS       DACCVU010000234         1997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCVU010000234 DACCVU010000000
VERSION     DACCVU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.242"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            123..1115
                     /locus_tag="IVW29_003026"
     CDS             123..1115
                     /locus_tag="IVW29_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7840430.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1131..>1997
                     /locus_tag="IVW29_003027"
     CDS             1131..>1997
                     /locus_tag="IVW29_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7840431.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLV"
BASE COUNT          598 a          329 c          404 g          666 t
ORIGIN      
        1 aaaaaaatat tgacttaaaa aaatggatat ggtaatctat aaacgaaaat agtggtaacg
       61 tttccaaaat gcatcttcgt taaattttta tttttttaaa cgaaaatgga aacgtttcta
      121 aaatgtattg ggaggagatt gaattggaaa agacattggg accaggtggt gagataaggg
      181 cggtacaacg ggataaaaaa aatatttcga agtgggaaaa attaaagaaa cagttgcggc
      241 gtgataagtg gatgtacttg ttgctcttac caggtatcat catgacgttt atctttaagt
      301 atgtgccaat gtacggtgct gtgattgcat ttaaagacta taatcctatg ctaggaattt
      361 tggaaagtcc ttgggtagga ctagatcact ttagggaatt tatcagttca ccaaatttta
      421 cgcttttgct gagcaacaca ttaaaaatta gtatttatgg tttattgtta ggtttttttc
      481 caccaattat tttagcactg agcttgaatc aaattatcaa taaaaaaata aaacagaaaa
      541 tccagttgat tctatatgcg ccgaatttta tctcagtagt tattattgtg gggatgctgt
      601 ttttgttcct atccgcaaat ggacctatta atgggttagt agaaaaggtt acggggcaac
      661 caattatgtt catgtctaat ccagcttatt tcagaacgat ttatatttta tcgggaattt
      721 ggcaaggaat cggctggtca tccattcttt acaccgcaac gctggcggga gtcagtaccg
      781 atttatatga ggcagcaaat atcgatgggg caaatatttt acaaaagatt tggcatatcg
      841 atttgcctgc attgaggcct gtgatggtga tcagcttcat cttggccgcc ggcggtatta
      901 tgaatgtagg atatgaaaaa gcctacttga tgcaaacttc aatgaacatc cctgcctcag
      961 aaatcatttc cacttatgta tacaaagtcg gcttgcaatc aggtgattat gcctactctg
     1021 ctgctgttgg tctgttcaat atgattatta atgtcgtttt attactgatt gtcaacaata
     1081 taaccaagcg gctaaacgat ggtcaaggct tgtaatgaaa ggagaaaatt atgaatagtg
     1141 ctttatcaaa aatcgaccgt caaatcttat ggttgaatcg agtgattgta acttttttag
     1201 tcgtcatcac cattgttccg ctgatttatg tactggtagc ttcttttatg gacccagcta
     1261 ctttgatcaa taaaggtata agcttcaatc cgaaagattg gacaatacag ggatatcaac
     1321 gggtatttgc agatgattcc attttacgcg gttttgtaaa ttcgttgttt tactctttta
     1381 gcttcagctt attgactgta tttattacga tgatcactgc gtatcctttg tctcgaccta
     1441 acttggcggg gaaaaagatt attatgactt tttttgtcat tacaatgttc gtgggtggtg
     1501 gcttgatacc tacctattta ttggtaaaga acttgggcat gttagatagt gtatgggcga
     1561 ttatcgtacc aggtgctata aatgtgtgga atattatctt agcccgtacc tattttagtg
     1621 ctttgccaaa agaactttct gaagctgcta ctatcgatgg cgccaatgac ttgcaaatac
     1681 tgttcaaagt catgcttcca ttagcgaaac caattatgtt cgtattgttt ttatatgcct
     1741 ttgttgggca atggaactca tactttgatg ccatgattta catcaaagat cccgacttac
     1801 agccgttgca actcgtatta agaaatattt tgatccaaaa ccaaccttca caagacatgg
     1861 tgggagccaa tactgccatg gcagaaatga aacaaattgc tgagttgatc aaatacgcaa
     1921 cgatcgtcat ttctagtctg ccattaatca taatgtatcc tttcttccaa aaatattttg
     1981 ataaaggtgt tttagtt
//
LOCUS       DACCVU010000235         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCVU010000235 DACCVU010000000
VERSION     DACCVU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.243"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            606..938
                     /locus_tag="IVW29_003028"
     CDS             606..938
                     /locus_tag="IVW29_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7840432.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1557..1898
                     /locus_tag="IVW29_003029"
     CDS             1557..1898
                     /locus_tag="IVW29_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7840433.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          690 a          268 c          421 g          614 t
ORIGIN      
        1 atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca
       61 agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt
      121 ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg
      181 gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg
      241 ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc
      301 tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac
      361 cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa
      421 gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga
      481 gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct
      541 ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt
      601 attttatgtc atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt
      661 gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc
      721 agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg
      781 ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa
      841 tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc
      901 gttgtaccgc aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt
      961 attttatcgt ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc
     1021 actagaaaaa taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt
     1081 aggttttaac gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat
     1141 agtggtgaaa tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag
     1201 ttagacaagt tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt
     1261 tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa
     1321 ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc
     1381 gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt
     1441 ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg
     1501 aaaaaattaa tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga
     1561 aaaattatga tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt
     1621 gggattacaa aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt
     1681 tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg
     1741 gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata
     1801 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct
     1861 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
     1921 agataaaggc ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt
     1981 gagtaaggaa ttt
//
LOCUS       DACCVU010000236         1984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCVU010000236 DACCVU010000000
VERSION     DACCVU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.246"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..335)
                     /locus_tag="IVW29_003030"
     CDS             complement(<1..335)
                     /locus_tag="IVW29_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7840434.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIA"
     gene            complement(895..1098)
                     /locus_tag="IVW29_003031"
     CDS             complement(895..1098)
                     /locus_tag="IVW29_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840435.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1298..>1984)
                     /locus_tag="IVW29_003032"
     CDS             complement(1298..>1984)
                     /locus_tag="IVW29_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7840436.1"
                     /translation="IAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHF
                     SRADIACDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKK
                     LEQERKKVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDK
                     IVIDGLIAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDT
                     WLLGLDVTEK"
BASE COUNT          558 a          461 c          311 g          654 t
ORIGIN      
        1 gcaatatctt ctccatattg atcgaataat tgccagccgt ttatcccaac tgatttcaaa
       61 tatccctgat tttcgttcaa acgcattaat tgctcgaaat cgacaaaaac aactccattt
      121 ttcgtccaat aagctatggt atcattcagt tttccgacaa ttgtaattcg atcaatcgac
      181 caatttaatt ttaggggggt ggtcgtacct tgcacactta accgcctgtt agaaatacgg
      241 ttaagttttt cgagctgatt tatcgttgtt cttttttctg cccctcgacc ttcaattttt
      301 gattgaattt cactcaactg attttcattt gccattttcg tcctccaaac gaaaaaagag
      361 acttttggaa agaatttcca gagttcacgt aaccctaaaa attcatatcc aaaaatctct
      421 agcttgaaga aacctaaaaa tattcgtata atgaacctga gttacggagc taggtgaagt
      481 agtttccgta aatagtcgag agtgttagcg cactgctcgg cttttatttt gctctttttt
      541 ctcgttttct ttgattatag cactcctcct aaaaaaataa aaagacctca gggaatttca
      601 ttccccgaac cccttgagcc tcctcctgcc gaaatggaaa gggcgcaccc gctgtttatt
      661 tcattcagct atcggttttc acaatctaac acgatcgttc cgctaccagt cgccttacag
      721 gctccatttt cgctcctcta acgtgtttta acgattgtgc cgataactaa acgaaataaa
      781 cgctaaaacc gctcagaacg caaaatatgc cattttagag ctattgtaga aaatgtagtt
      841 tgaaatgcaa ttttcttctt tcagcttttt atttcccact tgtaaatctg ctaattattt
      901 tctaccagac aaaccattga ttttttggct atcagttaac tcacgattat tatgagaaat
      961 gcttgtattc tttgttccct ttttgaacga aatactaaac cctaaggtga acccctcctt
     1021 cacgctgtcg ctaataaaaa gagtgtaaac ctttctgtta ctctttgaca aagagtaacg
     1081 ttttcctaat tgtgacatgg ctgtcacaat tccaaacgct ctccgaggga tttcgctgtc
     1141 acaagtgaca acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt
     1201 tattttgctt catgcaaaat attcgcttat cgtgaattta cgtcactaaa cattgacgca
     1261 aatccactct cactcatctg acttttctta ttcggtatca cttctctgtc acatccaacc
     1321 ccaaaagcca cgtatccaat tcttttttta attcgtctgc agattcctta aaagtctctc
     1381 gtaaatgatt cgttaactca tcattttgac tttcttgctt cagcaaattc cgatatttat
     1441 attttgtatg tctagcaatt atcgaccaat tagaaggctc agctatcaaa ccatcaataa
     1501 ctattttatc aataggtttt atatcaatcg gcaaaaaatg cgggcttgca aaactatcta
     1561 agctttcctg aaccatcgca tgccaatccg ttgctttgcc acgtcttagc tgcatttcta
     1621 accgccacca cgtatcgatt tcttttggca cgattacttt ttttcgttcc tgctctagct
     1681 ttttgttgta cagtcgcact tggcgctcac tggcacgact gccccagtac gctgtttcta
     1741 gcttgccaga acggccgtag attggcttaa aagaaatcgg gtctacaata cgatactgag
     1801 taataaagtc atccggcaca tctacaatat cgcaggcaat atccgcccgg gaaaaatgcg
     1861 gatctaaaaa taggtcatga atgaatcctt tcatggaact agctaaaaac tggcctatct
     1921 tattcggatt aaaatcaata cgaccttttc cttcatgaaa tttaagtatt tcgatatacg
     1981 caat
//
LOCUS       DACCVU010000237         1969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCVU010000237 DACCVU010000000
VERSION     DACCVU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.247"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..950
                     /locus_tag="IVW29_003033"
     CDS             <1..950
                     /locus_tag="IVW29_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HAP7840437.1"
                     /translation="NLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWG
                     SFGYALVALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAE
                     SNSIPSLKEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKI
                     YGTLNAIQVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCI
                     KMLHALEVPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNV
                     GYQPTFKIISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1004..>1969
                     /locus_tag="IVW29_003034"
     CDS             1004..>1969
                     /locus_tag="IVW29_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7840438.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYM"
BASE COUNT          634 a          277 c          400 g          658 t
ORIGIN      
        1 ttaatttagg cattattatg ggagcgctat ttttgtcagc tggatattta gcttctgtag
       61 gagtttttga agctgtgtca gaaaggttta gtcgtttatt tggctttgaa tatggacaag
      121 caagggcgtg gggatcattt ggttatgcct tggtagcgct tttggcagga tttttatttg
      181 taaaaaatcc tcatttaaac ttttgggcgg gatctttctt tggttcttta ctattgttaa
      241 atttattatt ttggaaccct aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac
      301 aagctgaatc aaatagtatt ccttctttaa aagaaatgtt tgatctaatg aaactgcctc
      361 aattatggac gataatcatc tttattgttt ttacatggac attttatacg gtattcgatc
      421 aacaaatgtt tccgggattt tatactggtt tgttttcaac atcagctaat ggtgaaaaaa
      481 tatatgggac attgaatgct attcaagtat tttgtgaagc gttaatgatg ggaattgttc
      541 caatcattat gagaaaatta ggggttcgaa atactttgtt attaggtgta accattatgt
      601 gtgtacgaat tggattgtgc gggtttgcct cgacaccatt atctgtttca tgcataaaaa
      661 tgttgcatgc tttagaagta ccattattta cattaccaat gtttcgctat tttacacttc
      721 attttgatac aaagctatca gcaaccctct atatgatagg atttcagata gctgctcaaa
      781 ttgggcaagt gattttatca acaccattgg gaatattaag agacaacgtt ggctatcaac
      841 caacatttaa aattatttct cttattgtat tactagcagg catatatgca ttctttattc
      901 ttaaacaaga tgatagagat gttcaagggg atccatttat tcgaggataa tggaaaccaa
      961 cagaataaaa aacaatttta tctagaaagt gagcgaatat attatgacaa aaagagctat
     1021 agaattaaca aatacacgtt atcgtttggg atatcatgtg tctgcaccat caggatggat
     1081 taatgatcca aatggatttt gctattttga tgggtattat catgtgtttt atcaacatca
     1141 tccatatagt gctgaatggg gaccaatgca ctgggcacat gctagaagta aggatttagt
     1201 tcattgggaa tctttaccac ttgctttgac tcctggagat caagaagatg aaggtggttg
     1261 tttttcagga agcgctattg aaaaaaatgg tgtgttgtat ctgttctata caggacatca
     1321 ctattatgga gacaatgatc cagatcattt ttggcaaaat caaaacatgg cgtatagtac
     1381 ggatggcatt cactttacta aatatgaaaa aaatcctgta atagcaaaag caccagaaga
     1441 taacacacat catttcagag atccaaaagt atggaaacac gatgaaaaat attacatggt
     1501 tttgggtagt caagaaaaag atggattagg tcgtgctatt gtttatcagt cgttagattt
     1561 attaaattgg gattacattg gaccaatttc taaagcgaag gaagtactta cagaaggatt
     1621 tatgtgggaa tgcccagact tttttgaatt aaatggaaaa tctattcttt tattatcccc
     1681 tcagggaatt gaagcaaatg gaaaagatta tttaaatcta tatgaaacag gctactttat
     1741 cggtgattat gattatgaga cagcaaaatt cattagagga aatttttatg aattagataa
     1801 gggacacgat ttctatgcaa cacaaacaac tttatctcca gatggtagac gtattgttat
     1861 ggcctggatg gatatgtggg aaagccctat gcctgaaaaa gtagacggat gggcaggagc
     1921 attaacgctt cctcgagaat tagagttaat tggagatcgt ttgtatatg
//
LOCUS       DACCVU010000238         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCVU010000238 DACCVU010000000
VERSION     DACCVU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.248"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..61)
                     /locus_tag="IVW29_003035"
     CDS             complement(<1..61)
                     /locus_tag="IVW29_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7840439.1"
                     /translation="MILKYPENICMLCIPMVMHK"
     gene            complement(306..1238)
                     /locus_tag="IVW29_003036"
     CDS             complement(306..1238)
                     /locus_tag="IVW29_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7840440.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            complement(1235..>1946)
                     /locus_tag="IVW29_003037"
     CDS             complement(1235..>1946)
                     /locus_tag="IVW29_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7840441.1"
                     /translation="PPNSLSTEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGG
                     FLGYVANRGNKYTFELAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVT
                     QYLVTTEFDDDTVQAIMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPR
                     TAQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYAD
                     LTSPYWQQHLVGAGRIKQ"
BASE COUNT          554 a          399 c          346 g          647 t
ORIGIN      
        1 ttttatgcat aaccataggt atacaaagca tacagatatt ctctggatac tttagaatca
       61 catgataaag gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc
      121 taattatttg ttcctgctat caaattgaca gtttatttaa gaataccttg ccgcatattt
      181 attaactcct tttaaatagt cacttaaata atagcacgta agagcatatt tgtaaaggaa
      241 tctccaattt tttatcaaaa agagtacatg attacaaagt atctgtaatc atgtaccaat
      301 atttgttatt ttacaatctt ccaattactc ccgttctttt caagtaccaa atcaaattga
      361 gatacctgcg ttgctttggt ctgctggtcg atatactcca ctgtcagcga taccgtgact
      421 tgattatcct tacgattgtg aataggattt accagttctt gaaagatgta ctcttttccg
      481 attggtttta atatcccgtc attcacatag taggaaagtt cactggctgt cgctgtagga
      541 tagagcttga agaacgtcgt taaaaactca ttgatttcat tggttgtaat ggaatcaacc
      601 gtcccctcac tttcaatggc ttttggttta taacttgatt tcttaggtat gttggtaatg
      661 gtcggattct taaccagtac catatttcca gaaccatcta catagacact cactatataa
      721 gcagagtgga cggtctttgt attttctccc tctgtaatga gctggtctac actgtaggtt
      781 acattaaact cattgtcgcc agttggctct accgtccata tctgaaatcc tcttacagaa
      841 gacgatacag gaatatcttt gcgtactgta tcaacattga gagcttgaag ttcatctgtc
      901 agatagcctt ttagactttc cattcgatta tcaatggact tatcggattg ctcccatgaa
      961 tagtagactt tcgcaaagtt ctctacaaaa ttttctacat gatgagtatc aacgtattcc
     1021 ttttctatga tagttgtttc gtgaatagta tgagtatcta tagctgtaaa gtgcttgaat
     1081 atcgcaaagc tgaaactaag ccctaaaagt acccacaagg caatcacaac ctttttatga
     1141 ggattgacct tatagacacg aggtttcttt tcctttggta tctgtttttc tttattctga
     1201 ttttttctaa atttcatcat taaatcttcc tttctcattg tttgattcgt cctgctccca
     1261 ctaaatgctg ttgccagtag gggcttgtta agtcggcata accgattggg tcgcctgcat
     1321 gaaacatacg gttattgcca aggtatatcc caacatgagt aatataagag ccagcgttat
     1381 aggtagaatg aaagaaaacc aaatcgccag cttgtgcttc cgatagtggg atatgctggg
     1441 tcacatcata ttgctgttgt gcggttcgtg gtaagttaat tccagctttt ccatacgtcc
     1501 attgtgtcag tccgctacaa tcaaaagaag tagtcgggga agctccaccg taaacgtatc
     1561 gccagccctc atatttcagt gcttcgtcca tgatggcttg taccgtatca tcatcaaact
     1621 ctgttgtgac aagatactgc gttaccagtt gcacataaaa catattgcca tagttgtatc
     1681 gccagccccc attgataggt atggctatgg gattggggta agacactttt tcgccacctg
     1741 aatactcttt tgagaaactt tgagccagtt caaaggtata tttatttcca cgattagcca
     1801 cataccctaa gaaaccacca ccataattgt aggactggat aaccgattct aaatctacac
     1861 tgagcctttc gctactggct aataattcac tgaaatactt cacaccttgc ttaatggatt
     1921 cttctgtact caatgaatta ggtgga
//
LOCUS       DACCVU010000239         1919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCVU010000239 DACCVU010000000
VERSION     DACCVU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.249"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            26..535
                     /locus_tag="IVW29_003038"
     CDS             26..535
                     /locus_tag="IVW29_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7840442.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            628..1790
                     /locus_tag="IVW29_003039"
     CDS             join(628..904,904..1790)
                     /locus_tag="IVW29_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7840443.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          674 a          349 c          384 g          512 t
ORIGIN      
        1 ttttattgtt tagggctata ctatattggt acaaggaata gaaagaggtg tttatgtggt
       61 tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac
      121 ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat
      181 tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg
      241 attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct
      301 tataagcatt tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata
      361 tggtcgaaag gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa
      421 tgaagcgtta gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa
      481 agtatcttat acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga
      541 aaaaaatgat ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat
      601 aaatgaaaac acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa
      661 gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt
      721 cgagattatg agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact
      781 ggctcgtttc aggaaaaaga taacttgacc cctgaacaag tagaactaat tgcattacga
      841 aagaaaaata aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg
      901 ggacgaaaag acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta
      961 aagtgttaaa catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat
     1021 cagaactgga agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca
     1081 ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc
     1141 gtatgatgaa acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt
     1201 cttctacttg taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa
     1261 agtcattaga agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt
     1321 atatctgcct gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga
     1381 agaaagatgc gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg
     1441 tacaactttt tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt
     1501 tatgggcctt taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg
     1561 ttgcagaatc cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga
     1621 cattggctaa tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc
     1681 tgcacggcac gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc
     1741 gaatccttga taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat
     1801 gagcttctgc cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac
     1861 cagcagcaag cttacgcaat accttataat ttttgtcaaa aaaagtgttg ccattccac
//
LOCUS       DACCVU010000240         1917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCVU010000240 DACCVU010000000
VERSION     DACCVU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.250"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="IVW29_003040"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="IVW29_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7840444.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="IVW29_003041"
     CDS             851..1039
                     /locus_tag="IVW29_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7840445.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="IVW29_003042"
     CDS             1052..1609
                     /locus_tag="IVW29_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7840446.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="IVW29_003043"
     CDS             1641..1883
                     /locus_tag="IVW29_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7840447.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          704 a          295 c          395 g          523 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaaga
//
LOCUS       DACCVU010000241         1766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCVU010000241 DACCVU010000000
VERSION     DACCVU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.252"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(110..1054)
                     /locus_tag="IVW29_003044"
     CDS             complement(110..1054)
                     /locus_tag="IVW29_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7840448.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(1056..1742)
                     /locus_tag="IVW29_003045"
     CDS             complement(1056..1742)
                     /locus_tag="IVW29_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7840449.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT          568 a          429 c          284 g          485 t
ORIGIN      
        1 ttaaataaca taacagatca aacttaatat attcttataa aaaaatagtg ttccactaca
       61 atgttagaag aggttgcgac aattattctg tcacaacccc tctcttctat tatttaaata
      121 gaatcatata agcaatttgg aaaactagca taaataacgc aataaacaac tggtttttga
      181 tcactccgta tttgtctttc atctccagca tcgctacagg cacgatattg aagttagcag
      241 ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa tgcaaccatt cctattaacg
      301 ctggattcgc tccatgacta aatacgaatg gtgcaccgat cccaaccgtc atcacggtga
      361 tggccgcaaa tgcattcccc atgattaccg taaatatcac catgccaaga gcgtaaatga
      421 tgatgccgat gttcacattt ccttctggaa cgatcgtacc tactgcattg gaaatcactg
      481 tcccaactcc agcgcttgta aataccgcac ctagagaagc taaaagcatt ggcaacatgc
      541 ttaacggccc tactgtgccc agcatatctg ctgcatcatc taaaaatgta cttggtcgat
      601 tatctcgaga aaagaacatc aagattagca tggctgcaaa aacaccgacg cccacgccga
      661 ctaaagcacc taaattggta aatagagcaa acaagatcgc aaagacaccg atactcaaag
      721 ctggaataaa aattttcatt cctagtttgt ctgacatttt ttccatatat tcttttgatg
      781 gaagccggct ttctcctttg ctgactttct tagcaattgc tggtatggtc atcgcaaaga
      841 tcaagccacc gttcacgatt gccggaagcc atcttcctcc tgcaataact acccctaaga
      901 ccgtccagaa aaaggctgac ccataacgat gaggattggt tttatctgtt aaatttctta
      961 ctccagtgta aatcaatacg agtcccatca cgatataaag gatctccagt aatttatcgc
     1021 ctaataaaat atcaggattt gtaaaaaagt ccatcttatt gcccctttcc tggataatat
     1081 ttcttcgcta atttcttttc tttaagatag aagtagacac ttgccacaac taaagccgtg
     1141 atagccactg gaacttccac taaagcaagc tgtcccaatg ttacgtcata tcctagatct
     1201 ttcagtgttg attggactag caacgcccct gctcctccga tgaatagtac ttggccaaag
     1261 aaccagcaga cattttccat agctgaagcc atacctttta gttcttcttc atgcttagga
     1321 tggacttcta acccttttga ttcgatggtc cccaatgcca tcggtaaaat gattggacga
     1381 acaaatccag caactccacc aaaactcacg ttaaaagccg caaagatcat tcgaaagatc
     1441 ccataaatat ctacaatcat accagcagat actttcttga atcgcttgat caaggaagcc
     1501 gctgattctt tcagaccatt tctttcaagc gttcctgttg ccaacataat gatgataaaa
     1561 atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa gttctaacat tccttgaatg
     1621 ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca ttacgatcaa tatcgagtct
     1681 aatttaaatg caaacccgag aataacaatc aagacgccta aaaggctaag ataattttcc
     1741 atatattttc ctccataaaa tgacaa
//
LOCUS       DACCVU010000242         1741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCVU010000242 DACCVU010000000
VERSION     DACCVU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.253"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..913)
                     /locus_tag="IVW29_003046"
     CDS             complement(<1..913)
                     /locus_tag="IVW29_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7840450.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNE"
     gene            complement(1072..1338)
                     /locus_tag="IVW29_003047"
     CDS             complement(1072..1338)
                     /locus_tag="IVW29_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840451.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1328..1684)
                     /locus_tag="IVW29_003048"
     CDS             complement(1328..1684)
                     /locus_tag="IVW29_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7840452.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          504 a          331 c          207 g          699 t
ORIGIN      
        1 tttcattttt taaaaaatac cttttaaatt tcttatagtc ctctgaaatc aaaatatagc
       61 gtgatggata ctcatcaaaa gaagaatcgt attcttcctt taaaattccc gatttactta
      121 atttttctag tacatcaaat actctatcac tcaagtaact ccttaaattg ttatcacttt
      181 cccattgaac tacttttcta taagttgaat tatcagttga aaagatagaa ttttcatcca
      241 taaacgcata tattagcagc aatctttcat cattgtcaaa ttctattgga tcaaaaatat
      301 ctaagttgtc atcaaaatct ttatcagttc cggtaacttt tttgttaatt actgaaggtg
      361 tcttgtaaga aatagaatct aaatcaaagc gttttgattc taaattattt tgtgctttct
      421 caattaaata attcactttt tttgtgagaa accttctgat attgtctttg ctatatgttc
      481 cataaaaacc cttatcttct aaagagtccc tgtacttctt aacttctata tactcttcag
      541 ccaaaacttg tggaactggt tcatctaaga aaaaaacccc tacctcgcct ttgctatttt
      601 tataaaagta atcaatttct gctatagttc ccgatctata attatcaact ggagaaccca
      661 acttatggcc aaaaatcgct aataatatat cactattttc tacaatttgt ttattaataa
      721 cactttgccc agagctatta tttatataag taggaatcgc attcgtttcc catcgaattg
      781 gcatcaaaat tacttttgta ttatctgtat tttctatatt ccatctatat atggtttctt
      841 ctacttcatt cctctttgct gctatatcac ctggtgatgc tataaatact tttattagat
      901 ttatggtttt catttattgt acctttctct ttatgtctct ttaaagtaat ttttgtataa
      961 aatttattga cttttacact ataggacaac ttttatctat ttagtacaaa aaggcccact
     1021 aaactaattg attagttagg agcctattac aggtatattt tcaactcgct tttaagcaaa
     1081 ttctactacc agctttttgc ctaatgccct agcgatatca tcaagtttat cgaaagatac
     1141 gttatcccct ctctcaattc gagcaatagt agattgagtt gtatgtgctt tttgtgcaag
     1201 ctccgcttga gttaggcctg cttcctcccg tgcatgataa agagcaaccg cagtttcgag
     1261 ttttcttcct tcacggtcat atgcttctgc aaaatctttg tctttttgct tttcttcaat
     1321 caatttttta atcatcattt gtgaaccact cctctctaag ttcttttgca tgtttaagtt
     1381 cacttattgg tgttttctcc gtttttttag taaatccatg ggtaatgaca taccttccat
     1441 tgaccacatg gaaatagata gctctttgga tgtttgatcc taccttactt cttaattcgt
     1501 acaaattagt gtctaatttt ttcacccatt tcaaccgttg agctactagc atcccattct
     1561 tttctgtttc ttctattgta cgtaaaagct tcgcactatc tttttttggt aatgtttcca
     1621 accattctaa aaattcatca tgaccattag gtcttttaaa tgtttcaaaa gatatttttt
     1681 ccatacccaa ttatatcata tattgatttt atattatagt atataacata aaaattcgaa
     1741 t
//
LOCUS       DACCVU010000243         1734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCVU010000243 DACCVU010000000
VERSION     DACCVU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.254"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            174..596
                     /locus_tag="IVW29_003049"
     CDS             174..596
                     /locus_tag="IVW29_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840453.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            606..809
                     /locus_tag="IVW29_003050"
     CDS             606..809
                     /locus_tag="IVW29_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840454.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1020..1604
                     /locus_tag="IVW29_003051"
     CDS             1020..1604
                     /locus_tag="IVW29_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840455.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          613 a          238 c          309 g          574 t
ORIGIN      
        1 tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa
       61 acgattatga cattaaaaat atattttatg aattttctag tatataactt gatttatctg
      121 ttaagattta ttaagaaaac gttttcttag ttttgatttc gaggtgatag acaatgaaaa
      181 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      241 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      301 taactccttt agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      361 ctgaaaatta tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag
      421 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      481 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      541 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      601 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      661 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      721 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      781 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      841 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      901 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      961 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
     1021 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
     1081 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1141 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1201 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1261 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1321 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1381 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1441 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1501 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1561 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1621 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1681 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atat
//
LOCUS       DACCVU010000244         1724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCVU010000244 DACCVU010000000
VERSION     DACCVU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.255"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            10..561
                     /locus_tag="IVW29_003052"
     CDS             10..561
                     /locus_tag="IVW29_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840456.1"
                     /translation="MKKFGAERIFIEKQSGATITHRPVFQEALDYVRDQDIFIVEAID
                     RLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPLLDKFIKDLIIQILAMIAEQ
                     ERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRLVYKSIVEDLKNGVAIAKI
                     AKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(726..1550)
                     /locus_tag="IVW29_003053"
     CDS             complement(726..1550)
                     /locus_tag="IVW29_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840457.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          570 a          296 c          251 g          607 t
ORIGIN      
        1 attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca atctggcgca
       61 acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga tcaagatatt
      121 tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat cgcttcagtc
      181 aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat catggctgaa
      241 gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca aattttggcc
      301 atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg aattaagatc
      361 gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga cgcaaaagat
      421 ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt
      481 gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat aaaaaaagac
      541 agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca tctttaattt
      601 cctacatata attactatct ataaaattaa gtcttactca gaacagataa agcataaaat
      661 tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat cttcacttat
      721 ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac aaaatcctta
      781 ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt tatactaatt
      841 cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa agtgatttga
      901 gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc tctatacata
      961 aagctatcat taattttata aaaaaaatta aatgttttta gcactgccat ataacattca
     1021 aaacattctt ctatagttgg tttacttcct tgaggagaat gaaccaattt attacgtttt
     1081 tctatgaaat ttttaatttc ctttcttatt tctttcggaa actcaatatt aaagtaccct
     1141 ttaaacatgc tccctaataa tttgtctaca gggggagatt gtaaattctc aaatattaga
     1201 gttaaatcag gtttctcttg ttgataaaat tcctttaccc ctatttcaca ggctgtaatt
     1261 atcgaaataa acctatctct agtatttgat atatttttac ttcttttaag gagtataaga
     1321 gaaaagggga tactttcgtg tccatgcatg taattaacta attgttgagc ttcagaagtt
     1381 atatctctaa ttatatagtt aacggaatta ggaagtgtct ttaactctaa tgaaatttct
     1441 atccaattac tatcttctaa tctgtatatg ctcgaagtat aatttgattc gtcagcaagc
     1501 atcgctacat cccaaacact gatcataaaa tcttttaaac gttcaatcat ttcagcaata
     1561 ttgctaaaat cagctaattt ttctttagaa taaaaaaaac atttatatca gaatcaataa
     1621 attctatttt ttttattact atatcgtcat cattatcttg accatttatg tttattgttc
     1681 cctgagtggg gagtacttct ttcaaattca agttaaattt atat
//
LOCUS       DACCVU010000245         1650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCVU010000245 DACCVU010000000
VERSION     DACCVU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.256"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..623
                     /locus_tag="IVW29_003054"
     CDS             <1..623
                     /locus_tag="IVW29_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7840458.1"
                     /translation="TKEGIQFEIQGMKETREADYYPGFSLRVLAHLENMRPDFKVDVT
                     TGDSIYPATITHSHKLMFEDRTIPLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLY
                     TIPKMEKIDEKTLKDSVRNTFTRRGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSN
                     PYAASITYDETMDSISNLMEKVIHVEEKERKLAYQRHQQMNREMER"
     gene            626..757
                     /gene="ssb"
                     /locus_tag="IVW29_003055"
     CDS             626..757
                     /gene="ssb"
                     /locus_tag="IVW29_003055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7840459.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            779..1111
                     /gene="ssb"
                     /locus_tag="IVW29_003056"
     CDS             779..1111
                     /gene="ssb"
                     /locus_tag="IVW29_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7840460.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            1133..1390
                     /locus_tag="IVW29_003057"
     CDS             1133..1390
                     /locus_tag="IVW29_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840461.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(1549..>1650)
                     /locus_tag="IVW29_003058"
     CDS             complement(1549..>1650)
                     /locus_tag="IVW29_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7840462.1"
                     /translation="SAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
BASE COUNT          577 a          287 c          350 g          436 t
ORIGIN      
        1 cgacgaaaga aggcattcaa tttgagattc aaggaatgaa agaaacgaga gaagccgatt
       61 actatccagg atttagctta cgtgtgctgg cacatttgga aaacatgcgg ccagatttta
      121 aggtagatgt gactacaggt gactcgatct atcctgcaac gatcactcac agtcataaat
      181 tgatgtttga ggatcgaacg attcctttgg aatcctatcc tacagaacaa attattgctg
      241 aaaaattaag tgcaaccttt gattttttaa cagataattc gagaggaaaa gatttttatg
      301 atctctatac tattcctaaa atggagaaga tcgatgagaa gaccttgaaa gatagtgtga
      361 gaaatacatt tacacgtcga ggaaaaactg atccgttaaa gatatactat gaaaaggata
      421 tggatgtact gaaaaatagt cacgagctaa aagatacctg gaagaaatat caaaaaagca
      481 atccttatgc tgcgtcgatc acgtacgatg agacaatgga ttcgattagt aatttgatgg
      541 aaaaagtgat tcacgtcgaa gaaaaagagc gaaaactagc ttatcaaaga caccaacaga
      601 tgaatagaga aatggagaga taaagatgat taataatgtg acattagtag gacgcttaac
      661 caaagatcct gatttacgtt ataccgcaag tggtactgca gtagcgactt ttaccctagc
      721 cgtcaaccga aatttcacga atcaaaacgg caattgagaa gccgatttta ttaactgcgt
      781 gatttggcgc aaaccagcag aaacaatggc gacattagct aaaaaaggta gcttgattgg
      841 tgtagttggg cggattcaaa cgcggactta cgacaaccag caaggacaac gtgtatatgt
      901 gactgaagtt gtagcagata actttcaact attagaaagt aaagcagcga cagagagccg
      961 tgcgcacgct gatcaatcgt ccaccagtcc aagcaccgcc acctttgaac aacggaatac
     1021 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
     1081 cgatattagc gatgatgatc taccgtttta gtttgagaaa gaaggagtaa gcatggcaaa
     1141 aaaaattagt ctgacgatgg atctattgat acccgtaatt gcatttatag acgggatttc
     1201 tttgggcgtc actgaaaatc ctttggtaaa tagaattgcg aaaggatcac tatttcttgt
     1261 attcgtgtat cttgtctgtt cttatctttt aaaaagagca ggaaaggata attccttttg
     1321 gcgattgact aaagtcacag tagcagcgac agctgttata cttacggcta ttctaccgct
     1381 ttatatgtag agttaacggg aaatcgttga acttcttgcg aaatattgct ataatgaaca
     1441 tgaaaaagag gagttgcgct aacaacccct cattaacagc accgattaaa gaacggtggc
     1501 agaatattag ttatttggaa ataaccgctg acctgctaaa gtagacggtt atttcttttt
     1561 gtcattttta agcatattta caatcaaact aatcaaagca atggtaaaca taccaaaacc
     1621 aagaatggtt tgaattgtct catatgcgga
//
LOCUS       DACCVU010000246         1633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCVU010000246 DACCVU010000000
VERSION     DACCVU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.257"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..681
                     /locus_tag="IVW29_003059"
     CDS             1..681
                     /locus_tag="IVW29_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7840463.1"
                     /translation="MIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNNLTIL
                     DTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLEN
                     QLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSL
                     NQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQ
                     IVNEHTIQ"
     gene            1109..1501
                     /locus_tag="IVW29_003060"
     CDS             1109..1501
                     /locus_tag="IVW29_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840464.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
BASE COUNT          687 a          185 c          239 g          522 t
ORIGIN      
        1 attattatta atgctggagt aattatagga ttgatgttag taattttctc cggaaagcaa
       61 tatgctgata atattttatt atggggaggg gaagataata ttgaaaagat caacaacaat
      121 ttaactatat tagacacagc tctagaaaat aaagagcaaa aaattagtat tttaaattca
      181 caattgtctt caaacaacca aactcttgaa caactaagaa ctaatattga agtttccaaa
      241 caaaaagtgg catctttaga aaacgacaag aatcaattag tatcagagaa aactaattta
      301 gaaaatcaat tgtcagcaaa agactctgaa ttgcaaggaa aacaaaatga aattaatgcg
      361 aaaatagacg aaattaacca aaaagtagct gaaataaatc gattaaatag cgattggaat
      421 aatcgtttac aaaatgaaaa gaacaaattt aatgaagaac aatctactat taatagttta
      481 aaccagcaaa ttaatcaaat gttgagtgaa aaaaataaca ttcaaaatca actgcaagct
      541 actcagcaaa agctagaaga agaaaagtca aaaaatagta gtttgaataa ttacattgaa
      601 aagcttcaaa aagctaaaaa tgatgtagag gataccgcaa ataaagcaca acaaattgta
      661 aatgaacata caatccaata gatatagttt taatttaaca aatggtatgt ttggagataa
      721 tgagatagaa tataaatgtc ataatattat acaatctaaa caccgtttcc ttttctattt
      781 ttgatgacat cagtatatag ctactcgaaa gagtagtctt tttattattc tcgcttatta
      841 ttgatatgaa aatttcgatt tgacaaaagg taccgaataa ttttgtaata tgaattatta
      901 tcaagaagta attatgatga taacatatgg aaactagtta gtaataataa caggtgatta
      961 aacttttgaa cgacttgtaa ttctatattt tttcttttaa gtaaaaaata taggtgattt
     1021 tttttgactt catatttata ataaagacac gtaaatcaat tatttttcat tttactttaa
     1081 ctaataatgt ttatttggag ggattattat gttagatgat agattgacgt gttctaattg
     1141 tggtacatta ttttactatg aggaagttag aaatattgta aaacatagaa aaaaagaaat
     1201 gcctatacgt tgtccaaatt gcaatgaagt tattaagcga aagttgtctc atggttattt
     1261 tgtcagctac gaactatatg aagaaagtaa aataaatctg aaaaaaaaac caaaatacta
     1321 taaaaatttg aaaactggag ctattataac tgaaaaagaa tttaaaaatt tagtgactga
     1381 agaagaaagc aggttttata atttgtctgg taatatttgc cctggattta tggatcagat
     1441 tattgaaaat gagtcaattg aatcaattga aaactatgtt cctgttgact taccaaaata
     1501 gaaaaaataa tcctgttttt acagtgaatt attttaattt tgattttcaa taaaaatata
     1561 ttacaataat aatcgtaata gctaatgaaa caatttctca atctttttac cactccaaac
     1621 agggagcgtc aat
//
LOCUS       DACCVU010000247         1606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCVU010000247 DACCVU010000000
VERSION     DACCVU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.258"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(18..304)
                     /locus_tag="IVW29_003061"
                     /pseudo
     CDS             complement(18..304)
                     /locus_tag="IVW29_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(294..623)
                     /locus_tag="IVW29_003062"
     CDS             complement(294..623)
                     /locus_tag="IVW29_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7840465.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(700..972)
                     /locus_tag="IVW29_003063"
     CDS             complement(700..972)
                     /locus_tag="IVW29_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7840466.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(972..1235)
                     /locus_tag="IVW29_003064"
     CDS             complement(972..1235)
                     /locus_tag="IVW29_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7840467.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1469..>1606
                     /locus_tag="IVW29_003065"
     CDS             1469..>1606
                     /locus_tag="IVW29_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7840468.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          513 a          313 c          200 g          580 t
ORIGIN      
        1 agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt
       61 ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt
      121 gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca
      181 cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat
      241 ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg atttcataag
      301 ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag tgtatcttca
      361 gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc tacaagaatc
      421 tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct aaaatcataa
      481 aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa ctttgcttca
      541 acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat tgaattgcct
      601 tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt aattacttag
      661 taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact cgtaatgcgt
      721 ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa caacccggtg
      781 ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact tctcaaatga
      841 ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa attgaggttt
      901 caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa tcatcacaga
      961 atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa tccactatca
     1021 tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa tagcgtttct
     1081 ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact gcgctcacta
     1141 atgatttcaa tttccgtatg attctcattt acatctttca acaacttata aaaatttttc
     1201 cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct aatttatatc
     1261 tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa attggagtac
     1321 caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta gttaattttt
     1381 tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat agagtagtct
     1441 caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg aacattatac
     1501 agatactttt ataacattca aggaaattat gagaactgtt tctaacatat accacaattg
     1561 tgtaccagat aagattaaaa accgaagaaa tactgatcaa ctgaag
//
LOCUS       DACCVU010000248         1605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCVU010000248 DACCVU010000000
VERSION     DACCVU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.259"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVW29_003066"
     CDS             131..505
                     /locus_tag="IVW29_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7840469.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVW29_003067"
     CDS             complement(619..1248)
                     /locus_tag="IVW29_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7840470.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVW29_003068"
     tRNA            complement(1382..1454)
                     /locus_tag="IVW29_003068"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          558 a          236 c          276 g          535 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataa
//
LOCUS       DACCVU010000249         1558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCVU010000249 DACCVU010000000
VERSION     DACCVU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.260"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            109..1278
                     /locus_tag="IVW29_003069"
     CDS             109..1278
                     /locus_tag="IVW29_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7840471.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          554 a          251 c          259 g          494 t
ORIGIN      
        1 ttaggatagc gtaaaatatt ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt
       61 ctgtaaaatt aaagttacca cacagaaatt ttataggagg actttctcat gactaaattt
      121 actatagaaa ttatgcaaac actactcaat aaaggggatt tagatgaatt atttcaggat
      181 catttagagc gagcgattaa ttcacttcta caagcagagc taactgcttt tttagattac
      241 gaaaaatatg atcgaaatgg attcaattca ggaaattccc gtaacgggaa ttattcacgt
      301 acatttaaaa cagaatatgg cgagttgaat ttaactattc ctagagatcg gaatggcgaa
      361 ttttctcaac aaacactacc agcatataag cgaaccaatg attcattaga aacgaccatt
      421 atccaattat ttcaaaaagg cattactatg gctgaaatct ccaaattaat tgaaaaaatg
      481 tatggtcacc actatacgcc acaaacaatc tccaacatga gtaaattggt ggctgaagat
      541 gttttagctt ttaaagaaag aacgttagaa gccaattatt ccgttatatt tatggatgcg
      601 acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag cagtgtatat tacgattggt
      661 attcgtttgg atggaaccaa agaagttctt gggtttacta ttgccccaac tgagtctgct
      721 tatatttgga aagaagttct tcaagatctt agaaaacgtg ggttagaaga agttttatta
      781 gtagtgacag acggattaag ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc
      841 caatttcaac aatgttgtgt gcatgtatct agaaatatcg ctcataaagt tcgtgttcga
      901 ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc aagcgaattc aaaagaagag
      961 gcattggatc acatcgactt tatgactagg aaatggaaaa agcagtatcc aagagtcgtc
     1021 aatttactct tgaatcctgc cctattaacc ttttataatt tccctcacgc catcagacga
     1081 acaatttatt cgacgaacct gattgaaggc tttaataagc agctaaaacg atatactcga
     1141 agaaaagaac aattccctaa tgaagaatct ctagagagat tcttcatttc tcaatttaat
     1201 caatataacc aaaaattttt aggtagaatt cacaagggat ttaaagaaat tcaggataca
     1261 ttagagtcga tgatttaact gtaattaaca gaatggattt tccatttaca tataattctt
     1321 gacgctacca taatctaaat aaccatcata ttcatctact acttatataa ttgagtttta
     1381 ttaaactttt taagtttcta ttctatcaaa tatttaaaca atctctgcat ccgaaaagac
     1441 tacctcgtat agttttacta acaatatttt tattagcgtg ttcaaatcct gatttcttgt
     1501 atcaattatt ttctgttcac tttcaataag aaaaaagtct ttacttccat ggataata
//
LOCUS       DACCVU010000250         1555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCVU010000250 DACCVU010000000
VERSION     DACCVU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.261"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..239
                     /locus_tag="IVW29_003070"
     CDS             <1..239
                     /locus_tag="IVW29_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840472.1"
                     /translation="DFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRG
                     TYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
     gene            246..758
                     /locus_tag="IVW29_003071"
     CDS             246..758
                     /locus_tag="IVW29_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840473.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1029..>1555
                     /locus_tag="IVW29_003072"
     CDS             1029..>1555
                     /locus_tag="IVW29_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840474.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          601 a          191 c          255 g          508 t
ORIGIN      
        1 ttgactttaa tagctctacc gaatttaagt tagcacttta tgcacaattt tttattgaaa
       61 aattaaatag gtttcaagaa atagttgaag atagtgattt taacactttt aatagccata
      121 aaccgatgag aggtacttat ataaaatttc tcgaaaatta taatgattta aaatctacca
      181 ataattatca atttggaaac ttgtcttatg agatgataag taaagttcta agtttgtgat
      241 gataaatgaa attaaatagt ataaatgatt tatttgctat tataggtgtt attgctaaca
      301 tacttttttc gtgtttgaat tttagattta ctcaaaagaa caaaaagaac aaactcagta
      361 tagaacaaat gaaaaaagac attgaaactg atttaaaaaa tgaccataaa gaaagaagaa
      421 aaattgaaga atatacttct ttaatggaaa agtgtaaagc aactgatgca atttttagct
      481 ttttaagaaa aagtgatgag ataatcacac gtgaaaatat agatttagaa aatgagatta
      541 gtaaacttga atctatcttt tccgatataa attctcttaa gtatttaatc gtcgaagaaa
      601 attcggatag ctttttagaa tttgaaaaat atctaacaga tcttatagat aatagaaata
      661 atgaattaat gagacaaaac attgcaagat tccttactaa attagtttct gaatggaaaa
      721 gtattatttc tcaaaatatg gataaattaa gatattaagg atgttagttt taaaataaac
      781 taatgtcctt atttttgtgt tcacatttct gttacctaag aaatacttaa aggaggtgat
      841 gtagccagca acgactacaa cttgaacccg ctatctacag tttatttgtt gtttcttttg
      901 ggtatactct aatggtacaa atccatattt gcttaaagcg ttgatttttc agcggttttt
      961 ctttattgca aagtgtaccc taagacctgt atactaatag taaatagatt ggataggaat
     1021 gatggcaaat gtatttagct tatttaagag tttcttccga cgatcaaagt ttagctcggc
     1081 aacataagct aattgaaaat tggtcagtca aaaataatat tgctgaagac gatttaaaaa
     1141 ttttcgagga aaaagtctct ggaaaaaata ttgaaaacag accagagctg caagagctta
     1201 tttctttcat acgtgaacga gatacgatta ttattcctag tcttgatcga ttagcaagaa
     1261 atagtaaaga tacaaaagac ttacttcaat tgtttcgtag taaaggtgct tctattgaaa
     1321 tattagacct accgtcgttt aatggggtaa ctgaccctgc attaagagac ttgctaacaa
     1381 atttagtcat agaagtgttt agctatgttg ctgaaaacga acggaaaaaa atcaaagagc
     1441 gtcaaaaaca aggcattgaa attgcaaaga aaaatggtcg ttatactggt aaaaagattc
     1501 aataccatgc caatgcaaca ggaaaaaaca aattgattta cgatgaaatt gttga
//
LOCUS       DACCVU010000251         1548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCVU010000251 DACCVU010000000
VERSION     DACCVU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.262"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..129)
                     /locus_tag="IVW29_003073"
     CDS             complement(<1..129)
                     /locus_tag="IVW29_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7840475.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFY"
     gene            823..1209
                     /locus_tag="IVW29_003074"
     CDS             823..1209
                     /locus_tag="IVW29_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7840476.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1303..1494
                     /locus_tag="IVW29_003075"
     CDS             1303..1494
                     /locus_tag="IVW29_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840477.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          577 a          238 c          216 g          517 t
ORIGIN      
        1 ataaaaaaaa taagtagttc tatctgagcg atactctttt ataatactga attcggaatt
       61 aaaaaccacg ataggtaact ttaaacaaac atgaagagac tttaataaga cttctttatc
      121 tgaaaacact tttgtttctc tctttccttt ctacatcatt aaatagtatt ttatacgtta
      181 tctaaaatac tattcctctg tcaaaataag ctaacttact tcattataaa atatccactt
      241 atcgctttca tgtatattcc accgtaaaaa gtacatttta tcaccgagaa ctcatcattt
      301 ttcatcatga tgaatactat ttacatatat ctagttcaaa gacaataatt attatgatcc
      361 gttattatct aagcactata cagataatag gaatttttcg tttctaaata ttccaatttg
      421 ggaatccaat aaaaattttc gttcaaatac gtgtctattc gaactctttt ctagttgtta
      481 attatgaagg gggaagaaat aaaaaataca atctatacac gaataagaat attatccaaa
      541 aggaggtttt agaaaagatt tattatacgt aaaaagctca agacattttc ttagccataa
      601 ggacatgttt gatatttaag aagatatatg aattttcttt tctaagtata aagataacta
      661 tccattatca actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg
      721 tgagcttata gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt
      781 tgcctctttt ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa
      841 cgcgtaaatg cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact
      901 tattataaaa aacaagaaga aattcaaaaa catgaacaac ttgatcaact cccaccatat
      961 ttgtttacta aagatactat aaaatttaaa caaccgatta atgttttaaa tgtgactctc
     1021 cttgttgaag atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt
     1081 tttcttatcg aaaaatttat ctttgataaa actgataaag aaattggaaa ggttctgggg
     1141 attacaagac agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg
     1201 ctggtttaat atctgcataa taactaattt tgtatgcttc tttgctcgtt ctcctccttc
     1261 ccaaagaaat agattataag tccatgggaa gaaggtagat ttatgaattc tttaaaagaa
     1321 gatttcattt tagcaaaggc tggaaatgaa gaagctgttg aggcaattct caaaagattt
     1381 agctctttga ttcataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa
     1441 gaatgtatgc ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac
     1501 taagggagtc tgtgacataa gtaaaaagat agtttcaaat ttcgaaca
//
LOCUS       DACCVU010000252         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCVU010000252 DACCVU010000000
VERSION     DACCVU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.263"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(183..755)
                     /locus_tag="IVW29_003076"
     CDS             complement(183..755)
                     /locus_tag="IVW29_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7840478.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(771..1376)
                     /locus_tag="IVW29_003077"
     CDS             complement(771..1376)
                     /locus_tag="IVW29_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7840479.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          425 a          328 c          216 g          548 t
ORIGIN      
        1 gtatcctaat atagtaagcg tggctagcat acctttaatt actttacctt ttacagttac
       61 catacacatt ttttgagcag aaatcaagta aataaaaaaa tattatattt cacaacatgc
      121 taatagactc atatctagaa cgcattaagt atttacctgt ctggttcgct gagacttctt
      181 ttttaacttt cttcaatttt cttctttatt ctaaaaagcg tactgcgact gaaaccggtt
      241 atagcttcaa cttccttata cgatttacct gaagtaagca gttcatatgc gtgcctcttc
      301 ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat taggattatt ctttcttgca
      361 aacatttttc cttcttgggt acgttccaca atcatatcac gttccatttc tgctacactc
      421 aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt caatcgttcc tagattcaaa
      481 accttgatcg ttacgttatc atcgagtaaa ggttcaatga tctctaatgc ttctctcgtg
      541 ttacgagcaa atcgatcaag cttcgtaaca actatttcat ctccaggctt cagaccagcc
      601 aacagctgat tgaattttgg ccgatcccgt tttgtacccg taaatttttc tgaaaaaata
      661 ttcttcttct ctacgcagtt ctcaagcaac tgttgaattt gttcttttgt actttgagaa
      721 gcggttgaga cacgtgcata tccatatttc gtcatttggt ccacctactt ttacaaatca
      781 tctatcgtat actcagaata cccttcatag aaatagctga catcgattcc tttcatatat
      841 aactcgcgat cgtcaatttt atcagtcaga gcattggaaa ttagatagcg tatttccaaa
      901 tcgtttatgg gacttcgctc cattgcagaa agatagtctg ctttatcgat taagttccaa
      961 tctacaactt tttgcaattc ttcctttaaa atgagatcca gccaaattct agtacttcgg
     1021 ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca catatttctt gataatctct
     1081 tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg aatgttctaa atacataact
     1141 ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc ggcaaaatcg
     1201 taaatatccg aaaatagaaa ttcatgaatt tcagataaac ctttaaatgt accaacttcg
     1261 atttcattta tttttccaca atcgtacaat tctttcgctt tttgtttact gagtttttct
     1321 tcaaccttag ccaactcaac ttgattggtt aatcccaatt tattctctaa aatcatgcga
     1381 aatctcctta actttatgac ataagtttat gaaacactct caaccccatt atatcgcgat
     1441 tttgtttatg tgtcaaaaac tattaagttt ctgacccccg atagattttt caagtgtctc
     1501 tattttgcac agtctag
//
LOCUS       DACCVU010000253         1462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCVU010000253 DACCVU010000000
VERSION     DACCVU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.264"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            238..1407
                     /locus_tag="IVW29_003078"
     CDS             238..1407
                     /locus_tag="IVW29_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7840480.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          533 a          214 c          238 g          477 t
ORIGIN      
        1 tttattacta tgtttgggtt ttatagcaaa gaaagtttaa taagagtttg gacctagcaa
       61 acaatattaa tgaggtcatt aaatcttatt atctttagtg atataacgaa atatctacac
      121 agacaaaatg atagcgtaaa atattttgtg taaatagata tttagggtag aaaaaaagaa
      181 agtccattct gtaaaattaa agttaccaca accgaaattt tataggagga ctttctcatg
      241 cccaatttta ctacagaaat tatggaaaca ctaatcaata aaggtgattt agatgaatta
      301 tttcgatgtc atttagaatt agctgttaat tcattattgc aggctgaatt aacagctttt
      361 cttgattatg aaaaatatga tcgtgctggg ttcaattcag gtaattcccg taatgggaat
      421 tattcacgtt cattcagaac agagtatgga gaattaaatt tggtaattcc tagagataga
      481 aatggtaagt tttctcaaca aactttacct gcttataaaa gaaccaatga ctctttagaa
      541 accactatta ttcagctatt tcaaaaagga attactatgt ctgaaatctc tgaattgatt
      601 gaaaaaatgt acgggcatca ctatacgcca caaaccattt caaatatgac taaaatagtt
      661 tctgaagata ttattgcctt taaagaaagg tccttagaat ccagatactc tgtcattttt
      721 atggatgcta ctcacattcc tttaaaaaga caaactgttt caaaagaagc agtttatatt
      781 gtaattggca ttcgattaga tggaaccaaa gaggttcttg ggtttagtct tgctccaact
      841 gaatctgctt atgtttggaa agagattctt caagacctaa aagaccgtgg tttaaaagag
      901 gttttattag tcgtaactga tggtttaagt ggcattaacg atagtattca tagtgtttat
      961 ccaaatgctc aatttcaaca atgttgtgtc catatatcta gaaatatcgc tcataaggtt
     1021 cgtgttagtg atcgacaaga aatctgtagc gatttcaaat tagtttatca agcaagttca
     1081 aaagaagaag caaataatca aataaggttt atgatagata aatggaaaaa gcagtatcca
     1141 cgagtggtta aattgctcat gaatcctgct atattaacct tttataattt ccctccgtca
     1201 atcagaagaa ctatttattc aactaatttg attgaaggtt ttaacaaaca attaaagaaa
     1261 tataccaaaa gaaaagagca atttcctaat gaagaatctc tggagagatt tctagtatct
     1321 caattcaaca actataatca aaagtttcta tgccgagttc ataaaggttt taaagaaata
     1381 catgatacat tagaatcaat gttttaactt gatcaacaga atgggctttt ccatttacac
     1441 aaaattattg acgctcccga ca
//
LOCUS       DACCVU010000254         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCVU010000254 DACCVU010000000
VERSION     DACCVU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.265"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..427)
                     /locus_tag="IVW29_003079"
     CDS             complement(137..427)
                     /locus_tag="IVW29_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840481.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IVW29_003080"
     CDS             complement(545..805)
                     /locus_tag="IVW29_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840482.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IVW29_003081"
     CDS             complement(942..1355)
                     /locus_tag="IVW29_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7840483.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          534 a          239 c          216 g          467 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaac cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatt
//
LOCUS       DACCVU010000255         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCVU010000255 DACCVU010000000
VERSION     DACCVU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.266"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            52..462
                     /locus_tag="IVW29_003082"
     CDS             52..462
                     /locus_tag="IVW29_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7840484.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="IVW29_003083"
     CDS             476..832
                     /locus_tag="IVW29_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7840485.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          531 a          210 c          268 g          428 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
     1081 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt
     1141 aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac
     1201 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca
     1261 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc
     1321 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
     1381 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DACCVU010000256         1427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCVU010000256 DACCVU010000000
VERSION     DACCVU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.267"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(189..983)
                     /locus_tag="IVW29_003084"
                     /note="aph(3')-IIIa"
     CDS             complement(189..983)
                     /locus_tag="IVW29_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7840486.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1076..1327)
                     /locus_tag="IVW29_003085"
                     /pseudo
     CDS             complement(1076..1327)
                     /locus_tag="IVW29_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          403 a          337 c          242 g          445 t
ORIGIN      
        1 aacagcgaaa tttttaaatc tatttcttat cgatacaaat tcctcgtagg cgctcgggac
       61 ccctatctag cgaactttta gaaaagatat aaaacatcag agtatggaca gttgcggatg
      121 tacttcagaa aagattagat gtctaaaaag cttgtagtta aagcttttta gacatctaaa
      181 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
      241 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
      301 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
      361 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
      421 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
      481 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
      541 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
      601 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
      661 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
      721 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
      781 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
      841 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
      901 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
      961 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     1021 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     1081 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     1141 tatcgacgga gccgattttg aaaccacaat tatgatagaa tttacaagct ataaggttat
     1201 tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat tctatagata
     1261 tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg gcgatatctt
     1321 ctatataaaa gataacccgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca
     1381 tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactccc
//
LOCUS       DACCVU010000257         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCVU010000257 DACCVU010000000
VERSION     DACCVU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.268"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            359..538
                     /locus_tag="IVW29_003086"
                     /pseudo
     CDS             359..538
                     /locus_tag="IVW29_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            600..784
                     /locus_tag="IVW29_003087"
                     /pseudo
     CDS             600..784
                     /locus_tag="IVW29_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            818..1249
                     /locus_tag="IVW29_003088"
     CDS             818..1249
                     /locus_tag="IVW29_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP7840487.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          472 a          223 c          289 g          423 t
ORIGIN      
        1 atgtgtgaaa taaaaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
       61 tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgagcaaag cccgaaaacc
      121 taagagaaag aaggagattt atatgaacaa aattattccc ttattattag tcctatttgt
      181 tggagaaata cttatgcgac ttagtgcagc tgtacattta aacctcgcag tatttttggt
      241 aatactaatg ttttgtttga tcttttggcg atttgtatta gccctcgtaa gtctactatt
      301 caagatgatt gcagccatat ttttcatagg attatttcga tgaaaggaga gagtaccaat
      361 gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga gacaaaaaga
      421 attgcagtaa ccaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt
      481 agatttattg attgcgcaag aaaaagcagg atttgacata ccagcactca aaaagtgatc
      541 gcactttgcg cacgtgttaa tacgtgttat aattattttg taaggaggta tagtgcacaa
      601 tgccacttac gggaaaagaa atgcttaagt tattgaaaaa aatggctggg ttgagcgaag
      661 gcaagaggga tttcatcatc atctttataa ggatggtgtt agaatcactg ttcctgttca
      721 tgccaatcaa gatcttggcc gaggacttga gcgaaaaatt ttaaaggatg cgggattgaa
      781 ataatctcgc attcgattaa agaaaggatt gactgaaatg ttaagtgagc ctttagcaaa
      841 aacctatcct gctattttta gcccagaaga aggtggtggg tactttattg aatttcctga
      901 tgttcaaggt gcttacacag gaattaacga agacgatata tcttatggaa ttgctatggc
      961 acaagaggtg ttaggtatgg ttttagcaga ttatattgag catgaagatc tacttccaga
     1021 accaacacca atcaataaga tttctgttga agatgattct tttactactt taattcgtgt
     1081 agatgttgcc aaatatttga aagacacaga acttgtcaaa aaaaccttaa caattcctca
     1141 atgggcggat aagctaggca aaagggctgg cataaatttt tcagtccttc taacagaatc
     1201 aattgcagat aaagcagata acattcttcg ttctggaaga aataattaga actcaaaagg
     1261 gtcagaaact taaaaatctt tggcttcttt ttttactaca aagatggctt ctttaaatta
     1321 tttgcctgat tgatttaaaa aaattgcgca aagactacga gctaattaag ttgctcgtgg
     1381 tcttttttta tgaaaacttt ttagatc
//
LOCUS       DACCVU010000258         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCVU010000258 DACCVU010000000
VERSION     DACCVU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.269"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1393
                     /locus_tag="IVW29_003089"
     rRNA            <1..>1393
                     /locus_tag="IVW29_003089"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DACCVU010000259         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCVU010000259 DACCVU010000000
VERSION     DACCVU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.270"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            78..>1356
                     /locus_tag="IVW29_003090"
     CDS             78..>1356
                     /locus_tag="IVW29_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7840488.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          462 a          262 c          257 g          375 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg
       61 agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata acagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg ttggtcgtcg
      181 atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact gtggtagatg
      241 gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc cgttttgaac
      301 aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc tgtaaaaact
      361 gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca atcgcaaatc
      421 atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt aaaagctatt
      541 taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa tttcgttcgc
      601 atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca ggaaaattaa
      661 tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta ggttgtacca
      721 atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc cagctcacca
      781 aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc aaacacatga
      841 atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa gccggacaga
      901 aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gtttccgagc accaaaatac ccatttctta
     1021 ctgaagcaat gatgattgat cgattgcttg agttttctcc gcccctaaag gaggcgtatc
     1081 ccttttttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1141 tattggcaga acttcccgaa acgttagatg acagctttcg ggaaaagctt caaaaccttc
     1201 tgacctatga agaaggcatc accaacgcaa tgatctatcc ttattccaat ggaaaaatag
     1261 aagcgaagaa tacccacata aagacaatga aacgagtatc ctacggattt aaatcatttg
     1321 agaacatgag aattagaatc tttttgatca atcaat
//
LOCUS       DACCVU010000260         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCVU010000260 DACCVU010000000
VERSION     DACCVU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.271"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            50..646
                     /locus_tag="IVW29_003091"
     CDS             50..646
                     /locus_tag="IVW29_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7840489.1"
                     /translation="MDGFERRTEEKKKKVLRAAFDLMNTDTGIENLTIDAVAKKANVG
                     KTSIFKYFGSKEKLIHEVFIRSMDEMGEKAKKIMEKNASFEETLIAMSQNKIDYLEKI
                     NKQFYLDLMSYYTKKEDSGFTRMMEAYTKQSFGIMLDLFHRGRKEGKVDLKYSDEFLM
                     LYFQAMIEGISNPQIYEKILPYTAEWTELLVKGIAPRQ"
     gene            complement(731..1228)
                     /locus_tag="IVW29_003092"
     CDS             complement(731..1228)
                     /locus_tag="IVW29_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7840490.1"
                     /translation="MIKMKYIKNVETLEELKKAYKKLAFKLHPDCGGNEEEMKILNNE
                     YDELFSKLKNTHKNKEGKTYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLT
                     GNTKPYKEDIKALEFRYSPKKYAWYKAPKDYKKRSRLRSICEKRLSLRQLHHILVVNM
                     YLKYT"
BASE COUNT          439 a          203 c          236 g          477 t
ORIGIN      
        1 gaactatgat aaacaaaaag ttctttttta tagaaagtag gtatttttaa tggatggttt
       61 tgaacgaaga acagaagaaa aaaagaaaaa agttttacga gctgcttttg atctgatgaa
      121 tacagatacg ggaatcgaga atttaacgat cgatgccgta gccaaaaagg ccaatgtagg
      181 aaaaacttct atttttaaat attttggcag caaagaaaag ctcattcatg aagtctttat
      241 ccggtcaatg gatgaaatgg gggaaaaagc taaaaaaatc atggagaaaa atgcatcatt
      301 tgaagaaact ttgatcgcca tgagtcaaaa taaaatcgat tacctcgaaa aaatcaataa
      361 acaattttac ttagatttaa tgagctatta cactaaaaaa gaagacagcg gctttacccg
      421 aatgatggaa gcttatacaa agcaaagttt tggcattatg ttggatttat tccatcgcgg
      481 gcgtaaagaa ggaaaagtcg atttaaaata ttcagatgaa tttttgatgt tgtattttca
      541 agccatgatc gaaggcatct ctaatccgca gatttatgaa aaaatcctgc cttatacagc
      601 ggaatggact gagctgttgg taaaaggaat cgctccgaga caatagtttt acgaacaaca
      661 aaagaagaaa tactcgccga atccacaaaa aaataaaatg tatgcataca aatcagaatc
      721 gattcatctg ctaggtgtat ttgagataca tattcacaac taaaatgtga tgcaattgtc
      781 tcagagacag ccgcttctca cagattgatc gtagtcggct tctttttttg taatctttag
      841 gggctttata ccaagcatat tttttaggtg aatatcggaa ttctagggct tttatatctt
      901 ctttatatgg ttttgtatta cctgttagcc atataaatgt tcctactact tcgattgcta
      961 cgccgtccat ttttagattg aacaatttat taataatatc cttaaactgt tcgggtgttt
     1021 ctgttgtttc tttggtgtaa gttttacctt ctttattctt atgagtgttt ttaagtttgc
     1081 taaacagttc gtcatattca ttgttcaaaa ttttcatttc ttcttcattt ccgccacaat
     1141 cgggatgtaa cttaaaagct aactttttgt aggctttttt caattcttcc aatgtttcaa
     1201 catttttaat gtatttcatt tttatcattc cttttctttt tgatttttgg ctttttgaca
     1261 accatttttg ttaggcgttt gaggaaatgt ttttataaaa aaagagtagt taccgttttt
     1321 tggtaactac tattttcctt ttttaaggtt taaag
//
LOCUS       DACCVU010000261         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCVU010000261 DACCVU010000000
VERSION     DACCVU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.272"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(854..>1355)
                     /locus_tag="IVW29_003093"
     CDS             complement(854..>1355)
                     /locus_tag="IVW29_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP7840491.1"
                     /translation="LNLPKTIYFNYKFLHISDAWRLPILVHRKVRITRKSRGKIEIVL
                     QSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFN
                     STGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNNKIVIGKHCWI
                     GAKSVI"
BASE COUNT          410 a          273 c          158 g          514 t
ORIGIN      
        1 tttaaaatta acattgattc tcctatgaaa atatctttta cctttctcct gtgaaaagga
       61 aatttccttt tttatagtgt tattgatata atcagaatat tcatcaaact ctgtatctgg
      121 agttaaaaat ctacagtaac atctataagc tttgctagta atcttcttgg gttctctctc
      181 cattgtcaag caacatcaaa acacctactt actattcgat tcttttctgc caaaaacgag
      241 tcagcatcac tcacaactta tgtgttgctg actccgacta gtcctttgtt caatatcaac
      301 ttattaaaca gatataatat ttttctttat ttcgtatact ccaagacttc catctttctt
      361 tcatcttatc tctgaaaaaa acacagcaaa gtgcccttaa taaatataca ttagaatttg
      421 caaatttttt catcttaaga tatatcctat tcatattctt tacaatccta atgggtgatt
      481 acaaatctat ttagttcaag ttctacatta taaacacctt tcttgatagt atcatctgct
      541 gcacaagcga tgtcgatcca cttattaggg gatttacgtg ctcaaaagtg gctattcctt
      601 ttgggaagtc catccataat aatggtccat ggattttttt agggtcttgt cgttcaaatt
      661 tctcaaaaac tttattatac ttccatagtt ttataattat ttggtttcac tttggctcgt
      721 tcaaacacca acttgtattt cttttcaacg ttcgaactgg agccccccct ataatctcat
      781 tattattttc atgacttttt actaccgtgg aattacaagc aacaacaata ttatctgcta
      841 aatgaacacc tttctatatc acgctttttg ctccaatcca acaatgttta cctataacta
      901 ttttattatt tttattcaaa atcctatgag tattcaattc aaaaatcgta tgtgaatcgg
      961 tatccataaa taatgtatcc cacgatactg tacagccatc tttgaattga atttccttaa
     1021 agcaaattat actcattttt cctgtcgaat taaactttga cccaatatct aaaactgcat
     1081 ttttccttat gtcaatatga gccccttgtg cgataaacgc tggcccttgt attcttagta
     1141 ttccttcaat acttataatc gtttcttcat ttcccgcagt atttaatact ggaaagccca
     1201 aataaactat ttttccagat tgtaatacta tctcaatttt ccctctcgat ttgcgtgtaa
     1261 tccgtacttt acgatgcacc aaaataggta atctccacgc atctgaaata tgaagaaatt
     1321 tataattgaa gtatatcgtt ttaggtaaat ttaat
//
LOCUS       DACCVU010000262         1344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCVU010000262 DACCVU010000000
VERSION     DACCVU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.275"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            172..843
                     /locus_tag="IVW29_003094"
     CDS             172..843
                     /locus_tag="IVW29_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840492.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          483 a          201 c          182 g          478 t
ORIGIN      
        1 cagaaaaata aaatactcct tctttttgga ctgattttat cgaaacttat tttttctttt
       61 tttcatgtat tcttttattt ttataactag tgtattactt caaaatttga tattatatgg
      121 catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg tatgacaatt
      181 aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat ctatgctgac
      241 ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc tattggtcgt
      301 acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga tacaattcct
      361 caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc tcgatttgtc
      421 agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa taaccaaaaa
      481 aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca aaaaaatgct
      541 cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat tgcagagcga
      601 gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt tcgatggcta
      661 cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta tcaactattt
      721 gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt gcatcttcat
      781 ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt caaaaatata
      841 taacttttaa ctctttttaa attaaatggt tgttctgatc agaaaaatat cgcttgtttt
      901 cttattatgt aatatatttc ataaactatt ctgcataaca actattgttt gtacttttta
      961 cattggataa ctaattatga ctgctgataa cttttgatat taaactagta tctccaatac
     1021 aaataaaaaa tagatatctt gttcatcaaa attagttcgt ggatacttac cttcatttac
     1081 atcattcagc ggtttcccat tttcttttat tctattattt cattttctaa tagacttggc
     1141 ttcatttcgc aatacttttt ttcaataggc atcaaattgg tgttgtttag atatttagtt
     1201 gtagttttgt gacatgataa gtatgctcct ttttctcttt tccactaata caacgatcaa
     1261 agggtgcttt tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt gattctatat
     1321 atcctgtttc aactacatct tata
//
LOCUS       DACCVU010000263         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCVU010000263 DACCVU010000000
VERSION     DACCVU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.276"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            278..820
                     /locus_tag="IVW29_003095"
     CDS             278..820
                     /locus_tag="IVW29_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840493.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            910..>1324
                     /locus_tag="IVW29_003096"
     CDS             910..>1324
                     /locus_tag="IVW29_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7840494.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACCVU010000264         1300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCVU010000264 DACCVU010000000
VERSION     DACCVU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.277"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            95..1054
                     /locus_tag="IVW29_003097"
     CDS             95..1054
                     /locus_tag="IVW29_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAP7840495.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
BASE COUNT          463 a          250 c          251 g          336 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat
//
LOCUS       DACCVU010000265         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCVU010000265 DACCVU010000000
VERSION     DACCVU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.278"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..279)
                     /locus_tag="IVW29_003098"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IVW29_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IVW29_003099"
     CDS             complement(545..739)
                     /locus_tag="IVW29_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840496.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          461 a          217 c          177 g          444 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagtt
//
LOCUS       DACCVU010000266         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCVU010000266 DACCVU010000000
VERSION     DACCVU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.279"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1244
                     /locus_tag="IVW29_003100"
     CDS             <1..1244
                     /locus_tag="IVW29_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7840497.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACCVU010000267         1261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCVU010000267 DACCVU010000000
VERSION     DACCVU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.280"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(263..1123)
                     /locus_tag="IVW29_003101"
     CDS             complement(263..1123)
                     /locus_tag="IVW29_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840498.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          386 a          238 c          183 g          454 t
ORIGIN      
        1 ttgtaaaaaa acaaataagg gaaacaaaca acacattttt gtgtgttgct tatttccctc
       61 taactatcgt tttctatttg atggttaacc tacttaaaat aaatactgca acgacaaacg
      121 gagatcaagc atgctttgta acgtatttta cgataattac gactttattt tattactcta
      181 tcttgctgcc atacccgtct atccctaatc atctttggtg aattttttca aagttagcat
      241 aacaaaaata agtatttttt ccttagttaa tcaatagtat catttttttc atatgattta
      301 attcatcaat taattttttg tttttattaa ttttagcaaa agctagtgca tcttgaatca
      361 attcgagcac ttctgccttc gacttatttt tagcctgtgc gttcttcgct aattgggcag
      421 cagctcgtgc taagtaataa gtcaaatggt tatccgaaca aatatcgatt gcgtaattta
      481 gtagtgcatc acttgtttcc aaatcaccgt cttgcgcaaa aaaaacacca caatgaaaga
      541 cgatatttaa cacccgccag atatcttctg ttgtacgaat cggatacgta taaatttttt
      601 caaacactcg attgaaataa acttctgctt tttccaactc tttttctcta gcatagacca
      661 ttccaatacc tgtatatgcc aataaacgaa acaacgtgtt ggctgtttct gccatcaaca
      721 actgatcaaa atggaacaac acatccgtaa ttggtgcatt cgtaaaaatc atttcaaatc
      781 cttttagata gtagtaacgt gcaatcagct cagactcgtc aattaccttg ggatcaatgg
      841 ctgctaaaat ctgtgtcaat tgttcatatt cactcgtaat cagtgaaaac tctgcttttt
      901 ctagctgttc atcaagttgt gtattgatca cgttcatcga agaaaaaagt tcaactaacg
      961 gaagattgat tcgattacat aatttgatta aaatttttaa cgaagggact tgaccattgt
     1021 tctcaaattt acttaatgtt gcttgtgtac aaattccttc agacacttct ttttgagata
     1081 agttggcagc ctttcttgcc gtaataaatt tttcaatgtt cattattttc tctccaatca
     1141 gacctcacac gaaaatcatt tctgtctcga ttttagcatg ttttctttgg gaatgcttta
     1201 ggttctgttg caaagtttta aataaagaat aaagtccctt acggtatcta tgatttaagc
     1261 t
//
LOCUS       DACCVU010000268         1254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCVU010000268 DACCVU010000000
VERSION     DACCVU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.281"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(58..981)
                     /locus_tag="IVW29_003102"
     CDS             complement(58..981)
                     /locus_tag="IVW29_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840499.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          398 a          226 c          129 g          501 t
ORIGIN      
        1 gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct tattaaatta
       61 tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg tttctgtcca
      121 tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta aactattaat
      181 atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct ggggagcata
      241 tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg tagctttggc
      301 taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt cttctctctt
      361 caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt catcttcatt
      421 ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt cttgactcat
      481 aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg ctaattctac
      541 cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct cagcattttg
      601 aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa tattttttaa
      661 aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt taataactct
      721 tccatgtaat aataagacaa aaatacataa atttttagct tccttattat tagatactaa
      781 ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca taaatctttc
      841 actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc catcttctga
      901 tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa aatttatctt
      961 ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt catatgacgc
     1021 tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca attcacactt
     1081 tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg cctttatcga
     1141 ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa agaataaact
     1201 aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct aaaa
//
LOCUS       DACCVU010000269         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCVU010000269 DACCVU010000000
VERSION     DACCVU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.282"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            13..1182
                     /locus_tag="IVW29_003103"
     CDS             13..1182
                     /locus_tag="IVW29_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7840500.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          444 a          232 c          204 g          365 t
ORIGIN      
        1 aaataaacta ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg
       61 atcgttgtcg tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa
      121 ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg
      181 atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat
      241 gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat
      301 ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt
      361 tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt
      421 tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact
      481 atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg
      541 atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag
      601 acctctcatt atgattcatt gaactttacc atattgactg gaattttgat caaattaaca
      661 aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat
      721 acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct
      781 aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta
      841 gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta
      901 tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga
      961 catccatata gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc
     1021 gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa
     1081 agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc
     1141 tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca
     1201 aatactactt ctttgaatag catttgctta aaaaaagtac tcgac
//
LOCUS       DACCVU010000270         1239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCVU010000270 DACCVU010000000
VERSION     DACCVU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.283"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(165..>1239)
                     /locus_tag="IVW29_003104"
     CDS             complement(165..>1239)
                     /locus_tag="IVW29_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7840501.1"
                     /translation="YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRAN
                     NIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLF
                     HEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVF
                     NLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAV
                     SLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSV
                     SETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVF
                     KKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          436 a          205 c          143 g          455 t
ORIGIN      
        1 tgtcatattc atttaacaca ctattattac cattacttag acgctcagat tctacgaaac
       61 aactcaaatc agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct
      121 ccaaactacg taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg
      181 aaattgtcca ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat
      241 actctcgaaa agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta
      301 tggtttgatt tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa
      361 atcgtaatat gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa
      421 taattagcta tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg
      481 acagaagaaa ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa
      541 taaacaatcc cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt
      601 tcttgtatca aagcatctct actttgatac gcttctatta tatccattcc taatgttata
      661 gacgtttgtg ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta
      721 tctccactaa cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt
      781 cgaatatctc catttttaac atattccagt attttatttt cataaagaaa cagatatatt
      841 tctggatcgt aattttgtga taacagatta tctattttga tttgctcgat agttttactt
      901 agattttctg tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa
      961 aaataatgaa ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt
     1021 tgcaatgaat tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt
     1081 agtttaaaaa aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg
     1141 gttttaaaca tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct
     1201 ttagtttttt taatattatc agttagtaac tttttataa
//
LOCUS       DACCVU010000271         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCVU010000271 DACCVU010000000
VERSION     DACCVU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.284"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            143..1183
                     /locus_tag="IVW29_003105"
     CDS             143..1183
                     /locus_tag="IVW29_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7840502.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          498 a          177 c          204 g          355 t
ORIGIN      
        1 aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg
       61 cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca
      121 acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg atagagttta
      181 caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat
      241 gaaagattca actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag
      301 caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg
      361 tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt
      421 tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa
      481 tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt
      541 tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca tacccaccct
      601 cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg
      661 ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt
      721 agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt
      781 agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt
      841 attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac
      901 tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag tttttgaaga
      961 actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta
     1021 tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa
     1081 aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaatta
     1141 tgcaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt
     1201 gattgttgct tcatggagta acattaaaat taaa
//
LOCUS       DACCVU010000272         1223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCVU010000272 DACCVU010000000
VERSION     DACCVU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.285"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..971
                     /locus_tag="IVW29_003106"
     CDS             18..971
                     /locus_tag="IVW29_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7840503.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            943..1218
                     /locus_tag="IVW29_003107"
     CDS             943..1218
                     /locus_tag="IVW29_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7840504.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          442 a          189 c          234 g          358 t
ORIGIN      
        1 ggaaattgga gggaactatg gaaaacttac ggactgcatt taaaacatca gataaactag
       61 gaaaagttgt aatgcttaca ggtagaaaag gcggcattgg taagactaca gacaatgatt
      121 tattagcgat tgtttctagt caactttttg aaaaagacgt gttgctaatt gattatgacc
      181 aacaaagaaa tactacttcc aacattggat caacgtatca aattactagc tttgatcgat
      241 ccatgtctgc agcaatcaaa aaaggggact gggtttcagg tattactcaa gtaagtccac
      301 atttatatat tatggctggc tctcctggat ctgaagaact taatgaatac ttgtccgaaa
      361 aatatcctga tcgtcgtaag agatcattgg ccttcattaa acctttagaa gaattgagaa
      421 aaaatttcga ttatatcttt atcgactgcc caccatcaac agataatgtg gttcgtgcat
      481 tcttaactgc tgcagattat attattgcaa tgcaagaatt aaaacggtac gcaatggaag
      541 gaacagaaga ttttattaat aaagttttag ttccgattgt cacgaatttt gaagagtctc
      601 atcttcaaat cattggaatt ttgcctgttc ttttctcagt gcgacgttcc tctcaacacg
      661 caaactatca aaaaacaatt gataagtatg gtgaatcaaa catttttaaa agcattgtta
      721 aaggctcgga tcggttagaa atgtatggtg agaatggtat ccaattaaat gattatgtag
      781 atcgaagatg gtgggctatt tttgcagaca tctttacaga attagaagaa agaattgaat
      841 actatgaaca acatggtgat attgaaggat ttgattatac acctaaatat gctgactcaa
      901 tggccaatca tatcctacct cttggaaagg agattaaact aaatggcatt attaccaacg
      961 aacaaagata agaagaaaat tggtgctgaa gatatttcaa ttaaagatga tattaatgtc
     1021 gagttagggg aacgaaagta cactgcgaaa gaaagaaaac cagttcaagt agatcctccc
     1081 gtactaaaaa ccattcggga tcttagttat gcaaaagata ttcctatgta tgaaatagtc
     1141 aaacttgcaa tggacgccta tgttgatact ttaaacgacg aggaacggat tatatataat
     1201 agacgatcaa aagattaaaa tat
//
LOCUS       DACCVU010000273         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCVU010000273 DACCVU010000000
VERSION     DACCVU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.287"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            70..1026
                     /locus_tag="IVW29_003108"
     CDS             70..1026
                     /locus_tag="IVW29_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840505.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="IVW29_003109"
     CDS             complement(1080..>1214)
                     /locus_tag="IVW29_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7840506.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DACCVU010000274         1208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCVU010000274 DACCVU010000000
VERSION     DACCVU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.289"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            78..>1208
                     /locus_tag="IVW29_003110"
     CDS             78..>1208
                     /locus_tag="IVW29_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP7840507.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTY"
BASE COUNT          398 a          229 c          234 g          347 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg
       61 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      181 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      241 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac
      301 aatacaatca catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact
      361 gtcgaaccca ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc
      421 atgtcagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaaattgtca ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact
      541 taccaaagca atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc
      601 acgcttccat agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa
      661 ttgatgtctt gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta
      721 atcctgaaga agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca
      781 aacgtattct gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga
      841 atcaagcctt taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga
      901 aaagtcaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta
     1021 cggaagcgat gatgattgat cgattgcttg gcttttctac gtccctgaag gaggcgtatc
     1081 cctattttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1141 tattggcaga acttcccgaa acgttagatg acggctttcg ggaaaagctc caaaaccttc
     1201 tgacctat
//
LOCUS       DACCVU010000275         1202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCVU010000275 DACCVU010000000
VERSION     DACCVU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.290"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..852)
                     /locus_tag="IVW29_003111"
                     /note="aph(2'')-Ia"
     CDS             complement(<1..852)
                     /locus_tag="IVW29_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7840508.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEI"
BASE COUNT          397 a          204 c          104 g          497 t
ORIGIN      
        1 aatttctttt ctaccatttt cgataaattc ctgtttaata tttttaattc cataaacaat
       61 agtttcaata ggataatatt cttcaactat atcttgatat tcttttgctt tctcaatatc
      121 tatatttcca tacattctta atatatcttc tccaaaattt gttcctattt cttcttcact
      181 atcttcaagt aagtatataa aatcacaata ttcatctata attccagaat ctccaaaatc
      241 aattattcca gttaatctat tattgccatc taacaataga tgattacaac taaaatcatt
      301 atggcataaa cactttttac cctcaaaaac tgttgttgca tttagtcttt ccataaaact
      361 ttctatataa tctttttcta tatcagttaa atcattataa atagtttcac gcaacaatat
      421 atactcttct aatacatttt gtttattatc aatagtacat tcactaatat ctgtataatc
      481 taaaccgtgc atttgtctta aaaaactggc aatatctcgt tttaacaaat tttgttcttc
      541 ttctgacata gtagaataaa tttctggtgt taaaaaagtt cctttaattt ctttataacc
      601 tagtatagat aattcatcac taatatacga atattcaata ttaggaattt ttacattagt
      661 ttctaaattt gtatttaaaa aattatatat tgctttttct tttgcataac cttttttctt
      721 attagtacta aattttgttt taaaaatgta ttcattatta actaaatatg ccacactatc
      781 ataaccacta ccgattattt caatactatc tactttgaaa ttatcaaagt aatgctcaat
      841 taaatatttc attgccttaa catttgtggc attatcatca tatctatatt ccattaaata
      901 acaatcttct tttttgccct cgtgtaattc atgttctggc aaatcttcaa taattctaaa
      961 accagatttt tggtatgccc ttattgctct tggattattt ttatgagggt ctaaaataac
     1021 tgcattagca tttctttctt ttttcaaaaa ttcaaaaatc aatttaatat atcttgtacg
     1081 gttctgttgc aaagttttaa ataaagaata aaatccctta cggtatctat gatttaagct
     1141 gggattccca ataatacctt gatttcagta cagaccgaaa acccgaagag agtgccttct
     1201 tt
//
LOCUS       DACCVU010000276         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCVU010000276 DACCVU010000000
VERSION     DACCVU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.291"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1076
                     /locus_tag="IVW29_003112"
     CDS             <1..1076
                     /locus_tag="IVW29_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7840509.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACCVU010000277         1189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCVU010000277 DACCVU010000000
VERSION     DACCVU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.292"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..383
                     /locus_tag="IVW29_003113"
     CDS             <1..383
                     /locus_tag="IVW29_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7840510.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IVW29_003114"
     CDS             complement(367..960)
                     /locus_tag="IVW29_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP7840511.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          358 a          237 c          210 g          384 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtatta
//
LOCUS       DACCVU010000278         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCVU010000278 DACCVU010000000
VERSION     DACCVU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.294"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(193..1008)
                     /gene="thiD"
                     /locus_tag="IVW29_003115"
     CDS             complement(193..1008)
                     /gene="thiD"
                     /locus_tag="IVW29_003115"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7840512.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          307 a          255 c          196 g          415 t
ORIGIN      
        1 gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag gctcacgacc
       61 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
      121 acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca actttttcac
      181 aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc catatttgtg
      241 ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca atcggatcgc
      301 ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata gcgaatcgcc
      361 agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg attttcctga
      421 ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat gatgtccttt
      481 gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca caaaatcttc
      541 ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag gtgtcagtac
      601 ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt ctagtaaaag
      661 atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat catatttttg
      721 gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg ttttagctgc
      781 acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat ctgttggcaa
      841 tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca tgacagaaac
      901 accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg cgcttccatc
      961 gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca aatcgattcc
     1021 tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata tgtttttcaa
     1081 agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat ttgaaaaaac
     1141 atttgcaata aatcagaaaa tagtggtcgt tta
//
LOCUS       DACCVU010000279         1171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCVU010000279 DACCVU010000000
VERSION     DACCVU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.295"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            99..1112
                     /locus_tag="IVW29_003116"
     CDS             99..1112
                     /locus_tag="IVW29_003116"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7840513.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT          404 a          195 c          264 g          308 t
ORIGIN      
        1 gcaaaaataa ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa
       61 tgataaaatg tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc
      121 taggtttttc ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat
      181 gggaagaagg agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc
      241 ttcattatgg tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg
      301 tcaatctctt tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc
      361 tgatgccaat ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa
      421 atgaagattt tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga
      481 ttggggtcgg tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct
      541 gtacgccagt aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg
      601 attacgatcg tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag
      661 cgagtttact tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg
      721 atccagccac acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa
      781 aagacggaaa attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt
      841 ccttgttgca gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca
      901 ttgatgatct agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc
      961 caatcggtgg gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag
     1021 gaccagtaac aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag
     1081 caccagaagg ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt
     1141 aaaaagaaaa aatcaggaag agactttaaa a
//
LOCUS       DACCVU010000280         1169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCVU010000280 DACCVU010000000
VERSION     DACCVU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.296"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            26..286
                     /locus_tag="IVW29_003117"
     CDS             26..286
                     /locus_tag="IVW29_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840514.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(648..887)
                     /locus_tag="IVW29_003118"
     CDS             complement(648..887)
                     /locus_tag="IVW29_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840515.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          414 a          164 c          218 g          373 t
ORIGIN      
        1 gaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata
       61 tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga
      121 aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga
      181 aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata
      241 tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag
      301 tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg
      361 ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc
      421 tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa
      481 actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc
      541 aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg
      601 agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt
      661 tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa
      721 acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg
      781 atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca
      841 gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat gtacacactc
      901 cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta
      961 tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc
     1021 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
     1081 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
     1141 gggtatttaa atagttgcta ggaggatag
//
LOCUS       DACCVU010000281         1142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCVU010000281 DACCVU010000000
VERSION     DACCVU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.297"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(419..>1142)
                     /locus_tag="IVW29_003119"
     CDS             complement(419..>1142)
                     /locus_tag="IVW29_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840516.1"
                     /translation="KKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNIIE
                     EIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAID
                     NVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPPT
                     WKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKKD
                     YSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT          374 a          202 c          144 g          422 t
ORIGIN      
        1 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
       61 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
      121 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
      181 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
      241 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
      301 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
      361 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
      421 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
      481 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
      541 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
      601 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
      661 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
      721 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
      781 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
      841 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
      901 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
      961 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     1021 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     1081 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     1141 tc
//
LOCUS       DACCVU010000282         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCVU010000282 DACCVU010000000
VERSION     DACCVU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.298"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..355
                     /locus_tag="IVW29_003120"
     CDS             <1..355
                     /locus_tag="IVW29_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840517.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..985
                     /locus_tag="IVW29_003121"
                     /pseudo
     CDS             615..985
                     /locus_tag="IVW29_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="IVW29_003122"
     CDS             985..>1137
                     /locus_tag="IVW29_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7840518.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DACCVU010000283         1120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCVU010000283 DACCVU010000000
VERSION     DACCVU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.299"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            234..527
                     /locus_tag="IVW29_003123"
     CDS             234..527
                     /locus_tag="IVW29_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7840519.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          416 a          165 c          193 g          346 t
ORIGIN      
        1 acccttggta ttcaagggtt cttttttctt agcatgtaat ttgaacaccc tgatccagtt
       61 ttttctttaa ttattggtag gggagaataa tggattagaa taaatctacc tataagtagt
      121 agtaaaaaaa agagcactat gataatatct ttcttaagag taaccgcttt aataaaaaat
      181 ccagaatatt ctaacataaa tggagtattc ttagaatcaa aaggagaaag aagatggttt
      241 atacttatac ggagcaagaa aagatgaaag ttttagacac attcatgaat gatggaaaag
      301 taaaacaagt accaagttca acaaaaaaga aatacatcct attgaaagaa gtcttgaaac
      361 gctttgacca tggagtgact tatacagaaa cagaggttaa ttcgatactg ctgaacgtat
      421 tttctagtgg cgattatgtc gaacaaagac gttatctaat cacatttggc tttttcaaaa
      481 gaagttcaga cggtcgggca tatcagatga tgggaattga aaattagaag ttttaattta
      541 tactcattga aaaaagttca agtatgcgat attggttcta caccgcatac ttgaactttt
      601 tttgtcatct gtatctataa tacaaagcca agaacgttat tcaaaatatg gatagctatg
      661 ataaaagcta atgagcgatt cgcagttaaa taagcaatcg tcagtaaaat cgttccagga
      721 gcgtacatga aaaaatcaat tggatgcgtt acatgaataa agataattct cttttatttg
      781 tacgaataaa taccaaaacg tacctcctaa aattagattt tggacagatg ccctttaaaa
      841 agataacaag atttcgttct tgaaacaata tatatgcttt tattccaatg aggttgtgaa
      901 aacagtattt tgtcctgagc gttagaaaat tgaaaatggg gaatagcaac tcgtacttta
      961 ccatttttta agtgaataca agatcagcta gttgaacacc gtttattcgt gatgagggac
     1021 acaacactgc agtcgacaca tttctctttt atgttcaaag ataacaaata tttaaaacaa
     1081 agaaaaatag tagataatcc cgtcctaact cttttattct
//
LOCUS       DACCVU010000284         1086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCVU010000284 DACCVU010000000
VERSION     DACCVU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.301"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            57..914
                     /locus_tag="IVW29_003124"
     CDS             57..914
                     /locus_tag="IVW29_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7840520.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          409 a          158 c          232 g          287 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaaatc cgcgatgtac attgcggact
      541 gggcaatcgt cgtcttacag acccaagtaa aatcgatgag aaatgcgatg caatatcttg
      601 agtatatgga cttttttgtg aaagaatttg atacaaattt atatgttgct ggtgttattc
      661 ctttcatgtt ggaaagcggt gacgcagtag atcaggaaat gtataaacaa gcccaagaga
      721 tttatgggga acatttgatt aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg
      781 acggatcagg tattacgatg gagaagacaa agaaaggtct cttaaaacaa tgggatcgca
      841 aaagccacga attatttatc agtattttga aagaattgga agaacacgag aattggtatg
      901 cggaggtgga gtaattggct gacatcaaag gacttgatat ttttgggaac aacaaaggac
      961 tcgccaaaat aaaaaaagaa gcagaagcaa agaaaaagca gcaagcaatt acaccatctg
     1021 aaaataagca attagaagtg caccaagttc aagaaataaa aacttcctcc aatgttgaaa
     1081 gacaga
//
LOCUS       DACCVU010000285         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCVU010000285 DACCVU010000000
VERSION     DACCVU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.303"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..544)
                     /locus_tag="IVW29_003125"
     CDS             complement(<1..544)
                     /locus_tag="IVW29_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7840521.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVL"
     gene            complement(620..766)
                     /locus_tag="IVW29_003126"
     CDS             complement(620..766)
                     /locus_tag="IVW29_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7840522.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(870..>1041)
                     /locus_tag="IVW29_003127"
     CDS             complement(870..>1041)
                     /locus_tag="IVW29_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7840523.1"
                     /translation="GPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGING
                     LYRADYGDKSQF"
BASE COUNT          409 a          167 c          109 g          356 t
ORIGIN      
        1 atagaacaac aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa
       61 atacttgata ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca
      121 tgaatatagc agtaagttta gaactggact gttcaacaat cctatttata gctaattttc
      181 tatataacca aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat
      241 aaccataccc ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaataa
      301 tagatctgaa atcatcaata agcatacata atagtaattt atttaaaagc actatattag
      361 ttttcttctt ttcctttaat ataaaataac ttccgattat aggtatagct gcggaaacat
      421 cagtaaataa ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa agatattttc
      481 ttttgttaag atcagtatta gtcatataaa ataagagaaa aaagaaaaag tttcccacga
      541 acattcttaa tagataagct tttgtatcga tagtaatcaa gagatcccct cctgatttta
      601 ggatatcata aaaaaaattt tagttacact ttttaaataa tgaaaaaaca cattccacta
      661 aatttgatgc cggttttcct tgaggttttg ttccagcacc tcctttaatt tttttaagct
      721 cttgatctga acattgttta gcatttagtc tatttttttc ttccattacg attcccccta
      781 caaactataa atacttttat gtcactgatt caatatacct atttttctaa tcaaaaaatt
      841 ttgagacata tttaatatta taagtatcat taaaattgag atttatctcc ataatctgct
      901 cgatataaac catttattcc agcagttctt ccaatttcac tcaaatttct tattaattcc
      961 tcttgatatt cagtgaatct tattttatta gtataagcag taaaatatag ataattcacc
     1021 aatctattta ttaaaggacc g
//
LOCUS       DACCVU010000286         1039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCVU010000286 DACCVU010000000
VERSION     DACCVU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.304"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            42..>1039
                     /locus_tag="IVW29_003128"
     CDS             42..>1039
                     /locus_tag="IVW29_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAP7840524.1"
                     /translation="MCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIR
                     FQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRL
                     AYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDS
                     TTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPN
                     PFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVT"
BASE COUNT          363 a          158 c          216 g          302 t
ORIGIN      
        1 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
       61 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      181 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      241 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      301 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc
      361 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
      421 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
      481 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
      541 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
      601 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
      661 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
      721 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
      781 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
      841 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
      901 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
      961 atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa caaggattgt
     1021 atggttggaa tgtgacgga
//
LOCUS       DACCVU010000287         1015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCVU010000287 DACCVU010000000
VERSION     DACCVU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.306"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..758
                     /locus_tag="IVW29_003129"
     CDS             <1..758
                     /locus_tag="IVW29_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7840525.1"
                     /translation="IFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRKSIISSCDQS
                     LQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNGEDTKKMTEI
                     LKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGAITFSPLAQGLLTNRYLHGIP
                     EDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLRQKSVTSVLV
                     GASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
BASE COUNT          356 a          175 c          198 g          286 t
ORIGIN      
        1 gtatttttag agaagatttt caagcctacc gtgatgaact gattatttct agcaaagcag
       61 gttatcatat gtgggaaggt ccatacggag aatggggctc tcgtaaatct atcatttcaa
      121 gttgtgatca gagtctccaa agaatggggc tagattatgt cgacatcttc tatcatcatc
      181 gtccagaccc tgatacacct ttggaagaaa cagcagaagc attgatgcaa cttgtaagac
      241 aagggaaagc attatatatt ggtatatcca actataatgg agaagatacc aaaaaaatga
      301 ctgaaatttt aaaaagaaaa gaagcaccgt tcattattca tcagatgcgc tacaatatgt
      361 tttctcgtga actactagaa gacgacttat ctccagtatt agaagaagaa ggtctaggag
      421 caatcacgtt cagcccgctt gcccaaggtt tgctgacaaa tcgttatctg catgggattc
      481 ctgaagattc tagagcacat cgtaaggaaa taccattcct ttcagaagaa caagttggat
      541 caactctgga aaaaatcaaa gcactgcaga caatcgccgt atcacgaggg caatcattag
      601 cgcaaatggc ccttgcatgg aatttgcgac aaaaatctgt tacgagtgta ctggttggag
      661 caagtcggtt aagtcagtta caagaaagtg ttcgaatgat ggataattta gatttctcac
      721 cagaagaaga attgaagatc gaccaaattc tagaatagaa acgatgtaat caaacttttt
      781 atccatctat caaaaaatgt catcaagaat cgaatgaagc tcatctatta ttgaacatca
      841 tacgttttgt cgttcaaaat aaaaatgatc gaaggctagg agtgaatttt atcctagtct
      901 ttttttatgg taaatagctt cgtttgtata tccatgaacg gatgaatcga tttttataca
      961 aaaaatcatc agatgattca aacgtgttat aataattacg aaaaaataca tcaga
//
LOCUS       DACCVU010000288         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCVU010000288 DACCVU010000000
VERSION     DACCVU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.307"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(18..90)
                     /locus_tag="IVW29_003130"
     tRNA            complement(18..90)
                     /locus_tag="IVW29_003130"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:His,seq:gtg)
     gene            complement(93..166)
                     /locus_tag="IVW29_003131"
     tRNA            complement(93..166)
                     /locus_tag="IVW29_003131"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Trp,seq:cca)
     gene            complement(173..253)
                     /locus_tag="IVW29_003132"
     tRNA            complement(173..253)
                     /locus_tag="IVW29_003132"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Tyr,seq:gta)
     gene            complement(255..330)
                     /locus_tag="IVW29_003133"
     tRNA            complement(255..330)
                     /locus_tag="IVW29_003133"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Phe,seq:gaa)
     gene            complement(366..438)
                     /locus_tag="IVW29_003134"
     tRNA            complement(366..438)
                     /locus_tag="IVW29_003134"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Asp,seq:gtc)
     gene            complement(448..520)
                     /locus_tag="IVW29_003135"
     tRNA            complement(448..520)
                     /locus_tag="IVW29_003135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Val,seq:tac)
     gene            complement(536..607)
                     /locus_tag="IVW29_003136"
     tRNA            complement(536..607)
                     /locus_tag="IVW29_003136"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Glu,seq:ttc)
     gene            complement(622..711)
                     /locus_tag="IVW29_003137"
     tRNA            complement(622..711)
                     /locus_tag="IVW29_003137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Ser,seq:gga)
     gene            complement(751..824)
                     /locus_tag="IVW29_003138"
     tRNA            complement(751..824)
                     /locus_tag="IVW29_003138"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Asn,seq:gtt)
     gene            complement(834..949)
                     /gene="rrf"
                     /locus_tag="IVW29_003139"
     rRNA            complement(834..949)
                     /gene="rrf"
                     /locus_tag="IVW29_003139"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          277 a          274 c          226 g          233 t
ORIGIN      
        1 accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
       61 ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac
      121 actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag
      181 agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact
      241 tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat
      301 ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag
      361 aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
      421 cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct
      481 gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc
      541 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
      601 acgggccagg taaatttgct acggagagta agggattcga acccttgaga cagtttggca
      661 ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa
      721 gaagctcaat actctttctc aaaatgagaa ctccggcagt aggactcgaa cctacgacat
      781 catgattaac agtcatgcgc tactaccaac tgagctatgc cggaataaca tcagcgtggc
      841 gacgtcctac tctcacaagg ggcaacccct cactacaatc ggcgctaaga agcttaactt
      901 ctgtgttcgg catggttaca ggtgtatcct tctcgctatc gccaccacac tgtggtgtta
      961 tcttttattg agtaaatttt gttcactcaa aactggattt gaagtcacat
//
LOCUS       DACCVU010000289         1008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCVU010000289 DACCVU010000000
VERSION     DACCVU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.308"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            101..>1008
                     /locus_tag="IVW29_003140"
     CDS             101..>1008
                     /locus_tag="IVW29_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP7840526.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYI"
BASE COUNT          374 a          181 c          183 g          270 t
ORIGIN      
        1 ataaagtccg tataattgtg taaaagtaaa aaggccatat aacagtcctt ttacggtaca
       61 atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac
      121 actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa
      181 aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca
      241 agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attatgagcg
      301 cagctttacg acacgtgtag gcacgctaga attaaaagta cccagaacac gtgatggcca
      361 tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca tggcttcaat
      421 gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact
      481 ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc tagaacctat
      541 ggttaacgag tggcagaatc gtttattatc agaaaaaaat tatccttact taatgaccga
      601 tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc
      661 gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc aaagtggcga
      721 aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt tacaaggtac
      781 ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa aatccttcac
      841 caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta ccaccattcc
      901 taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt tcacagatat
      961 taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcga
//
LOCUS       DACCVU010000290         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCVU010000290 DACCVU010000000
VERSION     DACCVU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.309"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            370..1003
                     /locus_tag="IVW29_003141"
                     /pseudo
     CDS             370..1003
                     /locus_tag="IVW29_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DACCVU010000291          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCVU010000291 DACCVU010000000
VERSION     DACCVU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.310"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(25..912)
                     /locus_tag="IVW29_003142"
     CDS             complement(25..912)
                     /locus_tag="IVW29_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7840527.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DACCVU010000292          993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCVU010000292 DACCVU010000000
VERSION     DACCVU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.311"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(155..228)
                     /locus_tag="IVW29_003143"
     tRNA            complement(155..228)
                     /locus_tag="IVW29_003143"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Ile,seq:cat)
BASE COUNT          369 a          155 c          161 g          308 t
ORIGIN      
        1 atacagaagt ataactatgt gaaacatcct ttcattaatc cataaaagga tacataaaaa
       61 agccactcat ttgagtggca atgaagaaaa gctttagctt gtataatact cttcaaaaaa
      121 ttctaacaca gaacgattca attggctaca ttaatgtacc ctgtaggact cgaacctacg
      181 accggacggt tatgagccgt ctgctctgac caactgagct aagggtagtg gttgttgcca
      241 cataaagcca taaacaatca accagtagaa tgcgtggcaa caaacctgtt atcgcatatc
      301 ttggagtgtg actatttatg agtgatagtg aagatatgcg tctccatata tgtatggttg
      361 agacagataa acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg
      421 agagaaaagt agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca
      481 aacatttaga aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa
      541 cttttgttcg tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat
      601 tatggtttaa gcatgttcct tcaaataagt tttttaatta taaactctag agaaattaat
      661 tcatcctaac ataaaataat tgttcactca aaagtttttc tagaacaatt aaaacagcag
      721 gcttcacatt cgctatacat ttgtaagaag taataattcc tgtactaaaa ggcataattg
      781 ctttaattat tttttgactt ctcttcaaac acaagaagta gttgtgtaat aattgcagaa
      841 ataaggattc caataataga tataaaattt gacatggaaa cgtctataac attaaaagaa
      901 taaagtgttg aagaaagtaa actgattgaa ataacccctc cgtaaaaaat agtctgtgaa
      961 tatacccact ttttttcaga agacgtactt cgt
//
LOCUS       DACCVU010000293          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCVU010000293 DACCVU010000000
VERSION     DACCVU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.312"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..>942
                     /locus_tag="IVW29_003144"
     CDS             5..>942
                     /locus_tag="IVW29_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7840528.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYP"
BASE COUNT          323 a          126 c          171 g          322 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagtcac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat cc
//
LOCUS       DACCVU010000294          921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCVU010000294 DACCVU010000000
VERSION     DACCVU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.313"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            397..843
                     /locus_tag="IVW29_003145"
     CDS             397..843
                     /locus_tag="IVW29_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7840529.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT          322 a          131 c          202 g          266 t
ORIGIN      
        1 aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt
       61 acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc
      121 tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc
      181 ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt
      241 cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat
      301 acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt
      361 atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt
      421 acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa
      481 gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc
      541 aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat
      601 gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt
      661 ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag
      721 catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca
      781 gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag
      841 taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat
      901 cagtaatcta taaaggggtt g
//
LOCUS       DACCVU010000295          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.317, whole genome shotgun sequence.
ACCESSION   DACCVU010000295 DACCVU010000000
VERSION     DACCVU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.317"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(207..>891)
                     /locus_tag="IVW29_003146"
     CDS             complement(207..>891)
                     /locus_tag="IVW29_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840530.1"
                     /translation="LCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQ
                     LEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDY
                     VPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSKGKTIVLPVFRYKGLT
                     VTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCI
                     KLKIRTSHE"
BASE COUNT          325 a          119 c          123 g          324 t
ORIGIN      
        1 gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta
       61 attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta
      121 agatttctct ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt
      181 agattgctaa aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg
      241 caataaaaaa gaagaacaat tagcgtgatt atagaaataa ataatgagat tttagaaaaa
      301 aatgtgtatg aatacctgat tgttacttta tttattccac tttcactttt tactgtaaca
      361 aaaggacctc cttgttttag tactgtagca ttttttgaat taacagtaac cgttaatcct
      421 ttataccgga atacaggtaa aactattgtt ttgccttttg aagatttaag cgtaaatgta
      481 atttcatctt tatttggaga aaagctacta taaataactt gaccatcttc tgtaacttga
      541 tggtttttca aaagcaattg atctttttta tagcctatag gtacataatc aaaattctca
      601 aattcttcaa gtttattttc catttgttca ttagaataag ttccttgaaa attcatttct
      661 ttcaccatgt taactactga tgaataattt aaaattgaaa tagctaatat taataatagt
      721 ataaaatttc ttctccttgt ataagaaaat ttttccaact gtggagaaat aaatagagat
      781 actgaaaaag atatgaacaa tgttgaaaat atcataaaac gccaaggagc ttgaataata
      841 tttaaaggag tattttgtaa ggcaaaccat ggaaatattt ttgtacaaag t
//
LOCUS       DACCVU010000296          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.318, whole genome shotgun sequence.
ACCESSION   DACCVU010000296 DACCVU010000000
VERSION     DACCVU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.318"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            200..311
                     /locus_tag="IVW29_003147"
                     /pseudo
     CDS             200..311
                     /locus_tag="IVW29_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            332..415
                     /locus_tag="IVW29_003148"
     CDS             332..415
                     /locus_tag="IVW29_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7840531.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            540..>891
                     /locus_tag="IVW29_003149"
     CDS             540..>891
                     /locus_tag="IVW29_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7840532.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFES"
BASE COUNT          336 a          120 c          147 g          288 t
ORIGIN      
        1 gtgacatctc tctattgtga gttattagtg gtacagtttt caaccgtttt aattataaaa
       61 aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt attgcaggtg tatttcttat
      121 ctatgggttt aacatggatt ttatcattaa aatcatgagt attgtccgag agtgattggt
      181 cttgcgtatg gttaacccta aagttatgga aataagactt agaagcaaac ttaagagtgt
      241 gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa ttagatgcta
      301 aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc gttatgtaga
      361 taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata aataaatacg
      421 ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac
      481 aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg agtgataaca
      541 tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa
      601 taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag
      661 ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca
      721 gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc actttaattc
      781 accaagatat tctacagttt caattcccta acaaacagag gtataaaatt gttgggaata
      841 ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc c
//
LOCUS       DACCVU010000297          889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.319, whole genome shotgun sequence.
ACCESSION   DACCVU010000297 DACCVU010000000
VERSION     DACCVU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.319"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            86..841
                     /gene="fabI"
                     /locus_tag="IVW29_003150"
     CDS             86..841
                     /gene="fabI"
                     /locus_tag="IVW29_003150"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7840533.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT          290 a          165 c          183 g          251 t
ORIGIN      
        1 ttaagaaatc aagaagttat tttagctatt attctctatt tttttacgac tatcaaattt
       61 ttatcagaaa caaaggagag tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat
      121 gggcgtcgct aacaaaaaaa gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg
      181 ggcagaaatt atctatactt accaaaatga acgaatgaaa aaatcattag tcaaattggt
      241 cggcgaagaa gcattcactg tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc
      301 aatgaatgcc atcggtgaat atgctgggga gatccacggc cttgtccatg cagttgctta
      361 cgccaacaaa gaagaattgt ccggaaatgt gtctgatatt tccagagacg gctatcaatt
      421 agctcaagat atcagcagct actctttaat tgctgtatca aaatacgcac aaccgtttct
      481 tgcaccaaac agcgggatcg ttagcatgac ttatctaggt tcagaacgcg caatcccgaa
      541 ttataacatg atgggtattg cgaaagcttc tcttgaagct gctatccgtt atttggcagc
      601 tgagttctcg cctaaaggaa ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt
      661 agctgtcaca ggtgttaaag actatcaaaa actgatccag ttatctgaat ctcgtacgcc
      721 tgatcagcaa ggtgtgacga tcgaagaagt cggtaatgct tgtgctttct taatcagccc
      781 actttccagc ggaatcatcg gtgatattat ctacgttgac aaaggtgtcc atctttcata
      841 aacttttgtt gcattcttta aagtaaaaca tataaaaatc aggaaaaaa
//
LOCUS       DACCVU010000298          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.320, whole genome shotgun sequence.
ACCESSION   DACCVU010000298 DACCVU010000000
VERSION     DACCVU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.320"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..596
                     /locus_tag="IVW29_003151"
     CDS             <1..596
                     /locus_tag="IVW29_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7840534.1"
                     /translation="KKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGG
                     GALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKG
                     QEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVN
                     GYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          242 a          159 c          209 g          244 t
ORIGIN      
        1 tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg
       61 gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta
      121 cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag
      181 aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat
      241 ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaaa cgcttaggga
      301 aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg
      361 ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg
      421 gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca
      481 atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca
      541 acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga
      601 aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata
      661 accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg
      721 agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac ttgtattgtg
      781 caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt ttaacaaata
      841 tatctttgct tctt
//
LOCUS       DACCVU010000299          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.322, whole genome shotgun sequence.
ACCESSION   DACCVU010000299 DACCVU010000000
VERSION     DACCVU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.322"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>845
                     /locus_tag="IVW29_003152"
     CDS             <1..>845
                     /locus_tag="IVW29_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840535.1"
                     /translation="FTVTDSIQNMIPDVLDLFNFIESTGLNNVYQSAVNTIVIGLMIL
                     SLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQFAKTFYKDSTTITN
                     SDDGVAWSLIKQGVTDLAYINKTDQYSSIDKTEDRNNLTRKNFQQTDLTQVLTDKVID
                     KLEKENPIADNLRYELVENSNNEFVATKFSDNFLSTFSDSLKSGYYRYQANLWGISIG
                     LTALAIAYVFSAFVIITAILELAFKRVLGVLVFATDIETGQRSKVVLSDILQCYLTVG
                     FQGFG"
BASE COUNT          283 a          122 c          162 g          278 t
ORIGIN      
        1 tttactgtga cagattcgat tcaaaatatg attccagatg tgctggattt gttcaatttc
       61 attgaaagta cagggctaaa taatgtttat caatcagccg taaatacaat tgtcattgga
      121 ttaatgatcc tttcattgat gtttgttggt tacaaaatga ttactggaaa aggaacagtg
      181 gatttaaaat cggttggaat gaacgtagtg atgtctatag cattaatact tttaatgcca
      241 accatgattt ctagtggtat tcaatttgct aaaacatttt ataaggactc tactacgatt
      301 acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac agatttagca
      361 tatattaata aaacagatca gtactcaagc attgataaaa cggaggatcg aaataattta
      421 acacgaaaaa attttcaaca gacggatctc acacaagtgt taacggataa agtgattgat
      481 aaattggaaa aagaaaatcc aattgcggac aatcttcgtt atgaattagt tgaaaattct
      541 aataatgaat ttgttgctac aaagttttct gacaacttct tatcaacatt ttctgatagc
      601 ttgaaatcag gctattaccg ataccaagca aatctatggg ggatttcaat tgggttgaca
      661 gcgttagcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat actagaactt
      721 gcatttaaac gtgtgttagg tgttcttgtt tttgcaacag atattgagac aggacagcgt
      781 tcaaaagttg ttctatcaga tattctacaa tgttacttaa ctgttgggtt tcaaggattt
      841 gggtt
//
LOCUS       DACCVU010000300          832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.324, whole genome shotgun sequence.
ACCESSION   DACCVU010000300 DACCVU010000000
VERSION     DACCVU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.324"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..67
                     /locus_tag="IVW29_003153"
     CDS             <1..67
                     /locus_tag="IVW29_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAP7840536.1"
                     /translation="IVSKNEIEEKITPVLQDKGVI"
     gene            93..>832
                     /locus_tag="IVW29_003154"
     CDS             93..>832
                     /locus_tag="IVW29_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7840537.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAG"
BASE COUNT          241 a          133 c          185 g          273 t
ORIGIN      
        1 tattgtctcg aaaaatgaaa ttgaagaaaa aatcacccca gttctacaag ataagggagt
       61 tatctaaata aaaaagaaaa gaggataaaa acatgggcgt tatcaatttt atcatcgaaa
      121 atattttgac tcaggcatcg atcaccattg cattgattgc tatgttgggt ctactgctgc
      181 agaagaaatc agcaggacaa gtcatttctg gcacgttaaa gacactccta ggattccaag
      241 tgttgagtgc cggttcgagt atcatcgttg gaagtttaac ttatttcggg aaaattttta
      301 ctgaaggatt tcatatgcaa ggaatcattc cttctattga gtctatcaat ggtcaagcaa
      361 tgaatgattt aggtttaggt agagatattg cactgacgtt cttagcgatt tttgtattca
      421 atattatttt ggctcgtttc acaaaatgga agtacatttt tctaactggt caagccattt
      481 tatggatggc gacgatgacg acggtctttg gttatttcgc tggtctgcga gggatcgtat
      541 tgatcttagt cggtggtttt atcggagctt gtttcgccat tgctatgcca gcagtagcgc
      601 aaccaattat acgtaagatt acaggatcga atgatattgc tttagggcat ttttgtacga
      661 ttggatactt atttgaagct ggcgtggcta aactttttgg tgagaaaggt gaaaataaaa
      721 aatcgattga agagatcaaa ttgcccacgc atttcgagtt tctgcaagat acgtatttgt
      781 ctgtcatggt cgtgatggtc ccactatata tcatcactgt tttgtttgca gg
//
LOCUS       DACCVU010000301          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.325, whole genome shotgun sequence.
ACCESSION   DACCVU010000301 DACCVU010000000
VERSION     DACCVU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.325"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..>823
                     /locus_tag="IVW29_003155"
     CDS             22..>823
                     /locus_tag="IVW29_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7840538.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACCVU010000302          821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.326, whole genome shotgun sequence.
ACCESSION   DACCVU010000302 DACCVU010000000
VERSION     DACCVU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.326"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..750)
                     /locus_tag="IVW29_003156"
     CDS             complement(37..750)
                     /locus_tag="IVW29_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7840539.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          260 a          169 c          107 g          285 t
ORIGIN      
        1 aaaaaaataa gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc
       61 aaaaacaaag tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga
      121 tagagcaaca actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt
      181 tgcgtcaaat tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag
      241 acccagttca tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc
      301 tggtatgact gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa
      361 acggcttcgt acaatattat atacatagtc ttttacactg atacttaatt tatcagctac
      421 ttttttcgat ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc
      481 aaccacgcta aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct
      541 gacgacttca gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc
      601 tacgcctttc tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt
      661 aggaagtttc tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta
      721 aatttccata tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc
      781 atctatactt taacataaat aaaccgataa aatccacatc a
//
LOCUS       DACCVU010000303          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.328, whole genome shotgun sequence.
ACCESSION   DACCVU010000303 DACCVU010000000
VERSION     DACCVU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.328"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IVW29_003157"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IVW29_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7840540.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACCVU010000304          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.330, whole genome shotgun sequence.
ACCESSION   DACCVU010000304 DACCVU010000000
VERSION     DACCVU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.330"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            70..762
                     /locus_tag="IVW29_003158"
     CDS             70..762
                     /locus_tag="IVW29_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7840541.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          353 a          129 c          144 g          178 t
ORIGIN      
        1 acgcttgaca ttgtgtttga gatcacttac ttttagcatg taaaagtatt ggattaagga
       61 gctatgcaaa tgaaaaagaa atttattact ggtgggattg tcgttgcatt tctatgtcta
      121 attggctaca caggcacgcg gtacgcaaat gaaattattt tgtggggtgg agagacagat
      181 attgatacaa tcaatgagaa tctgacacag cttgatcata cattgacaga taaagagcaa
      241 gcgattacag atcttaccgc tcgactagca caaaaagaac agacattgca agattcaaag
      301 gcagatgtcc aagaatacca aacaaaagta aatgaattag aaaaccaaaa aaatcaatcg
      361 gcagcagaaa aacaaaactt agaaagtcaa ttagtcagca aggatgcaca actacaaagc
      421 aaacaaaatg aaatcaatgt caaactagat gagatcaacc aaaaaaatga tgaaatcaat
      481 catctgaatg cagattggac agcaagacta caagaagcac aagcaaaact ttcagaatta
      541 caaaatactg taaatggttt gaatcaacaa atcgcaacac tgacacaaga aaaaacaaat
      601 gtagaaactc agttaacaga tacacaaaag aaattagacg aagcaactgc tgaaaactca
      661 agcttgaaac aatatattca aaagttagaa aaagcaaaag atgaagtgga agatacagct
      721 gataaatctc agcaaattgt tgaagaacat ttgcgtaaat aatctttctt aacaagtaaa
      781 cctaaattag taaagaagca acag
//
LOCUS       DACCVU010000305          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.333, whole genome shotgun sequence.
ACCESSION   DACCVU010000305 DACCVU010000000
VERSION     DACCVU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.333"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..>764)
                     /locus_tag="IVW29_003159"
     CDS             complement(109..>764)
                     /locus_tag="IVW29_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP7840542.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          175 c          146 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccga
//
LOCUS       DACCVU010000306          759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.334, whole genome shotgun sequence.
ACCESSION   DACCVU010000306 DACCVU010000000
VERSION     DACCVU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.334"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            75..260
                     /locus_tag="IVW29_003160"
     CDS             75..260
                     /locus_tag="IVW29_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7840543.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            273..521
                     /locus_tag="IVW29_003161"
     CDS             273..521
                     /locus_tag="IVW29_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840544.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT          279 a          104 c          164 g          212 t
ORIGIN      
        1 aaaagctaat aggtgtcaac gatttagcta attttttagc ttacaaatta tttgtttaga
       61 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
      121 gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag
      181 atattctaaa cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa
      241 aaatattgga catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg
      301 taaaaaaata taattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata
      361 cagcaatttt ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag
      421 aattatacga taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa
      481 cacctaaaga atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga
      541 ggtgaaggtt aatgggtaaa ttcaacagag cattagtatt cagcgcaccg ctaatcgtct
      601 atgctttagg gctttgggga agtagacaag cgttgatagg aacgattgtg tacatggttt
      661 ggatttttat agggctggat gaagctgagt acaaaacaaa aaagccagtc gggagggact
      721 gactaatgag taaggacgat attgtatttc tgttaatta
//
LOCUS       DACCVU010000307          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.335, whole genome shotgun sequence.
ACCESSION   DACCVU010000307 DACCVU010000000
VERSION     DACCVU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.335"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(348..548)
                     /locus_tag="IVW29_003162"
     CDS             complement(348..548)
                     /locus_tag="IVW29_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7840545.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          276 a          119 c          116 g          244 t
ORIGIN      
        1 attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa atctgcatat
       61 tcatcactaa tattttttct aataaattct tccatcataa agatcacctc gaaaagagta
      121 tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa taggtgaaca
      181 agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag
      241 aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg tgtaatagtt
      301 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      361 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      421 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      481 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      541 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      601 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      661 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      721 tacaactttc cttttcaaag taccatataa aaaaa
//
LOCUS       DACCVU010000308          752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.336, whole genome shotgun sequence.
ACCESSION   DACCVU010000308 DACCVU010000000
VERSION     DACCVU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.336"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>752)
                     /locus_tag="IVW29_003163"
     CDS             complement(<1..>752)
                     /locus_tag="IVW29_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7840546.1"
                     /translation="LIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPT
                     VLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKY
                     LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKK
                     YRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQL
                     LRYYFVKRGTEGFQETLKTISSTLPQSFKKKF"
BASE COUNT          213 a          140 c          165 g          234 t
ORIGIN      
        1 tgaatttctt tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa
       61 agccttccgt acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag
      121 ttcgcaattc ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc
      181 gtttaaacag tcgatgataa tgccgtttga gtggctccaa tgtcgtggaa tctttgagta
      241 agagtttcca atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc
      301 gaaaacgatt catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa
      361 tatgaaagcg gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat
      421 agctggcatt catgtccatc actaggtact tgacctttaa acgggctttt ctaggaaagt
      481 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
      541 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      601 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      661 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      721 tacgtgattg attttcgtac agttctatga ga
//
LOCUS       DACCVU010000309          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.337, whole genome shotgun sequence.
ACCESSION   DACCVU010000309 DACCVU010000000
VERSION     DACCVU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.337"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            192..>742
                     /locus_tag="IVW29_003164"
     CDS             192..>742
                     /locus_tag="IVW29_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7840547.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNN"
BASE COUNT          257 a          110 c          134 g          241 t
ORIGIN      
        1 aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta acctacaata
       61 ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat cttctttggc
      121 ctttttttga taaaaaaaat actagatgta agataaaagt gtaaaaacga tttgttttag
      181 gacgggaaac gatgtgtacc gcaatcactt atgcaacgaa agaccattat tttggaagga
      241 attttgatta tgagatgtct tataatgaag tggtcactat tactccaaga aattatcgtt
      301 tcgattttag aaaggtaaaa aatttagata aacattatgc aatgatagga atagcagctg
      361 gggtatccaa ctatccactc tattatgagg ctacaaatga aaaagggttg agtatggcag
      421 gactgaattt tcctggcaat gctgattata aagaattaca agaaggaaaa gataacgttg
      481 caccatttga gtttattcca tggatactag ggcaatgctc aaccatagac gaagctaaag
      541 aattattggc tactatcaat ctagtcaata tcgattttag tgaaaaacta cccttgtcac
      601 ctttacactg gttattagct gataaagaaa aatcgatagt aattgaaagt atgaaagatg
      661 gacttcattt atatgataat cctgtcggtg tgttgacaaa taatccgcca tttgattatc
      721 aattatttaa tctgaacaat ta
//
LOCUS       DACCVU010000310          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.338, whole genome shotgun sequence.
ACCESSION   DACCVU010000310 DACCVU010000000
VERSION     DACCVU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.338"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>734
                     /locus_tag="IVW29_003165"
     CDS             <1..>734
                     /locus_tag="IVW29_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7840548.1"
                     /translation="FQLQEMPQVFHFDSAGFWNPFTPTTSLGTRLAISTVCVVPAVLM
                     GLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQIPETKTTFPGYGGIPITHYKKYWYI
                     QTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAEKQSSMVVNDPKTELYVGSKDELEAR
                     GYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANASDNKY
                     FNENAQKAVNALIFALLEQADQTGDYS"
BASE COUNT          249 a          132 c          141 g          212 t
ORIGIN      
        1 tttcaattac aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca
       61 tttactccta caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca
      121 gctgtactta tgggattgaa aatggacatg tggtttcgtc cgctagcaga tggacaaaaa
      181 ggggataatc gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa
      241 acaaaaacta cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg
      301 tacattcaaa cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt
      361 cagattgaag tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg
      421 gtcgttaatg atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga
      481 gggtatgatg tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca
      541 ttggcactca tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc
      601 aatactttca caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac
      661 gaaaacgcgc agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa
      721 acaggagatt actc
//
LOCUS       DACCVU010000311          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.339, whole genome shotgun sequence.
ACCESSION   DACCVU010000311 DACCVU010000000
VERSION     DACCVU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.339"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>714)
                     /locus_tag="IVW29_003166"
     CDS             complement(<1..>714)
                     /locus_tag="IVW29_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7840549.1"
                     /translation="AVEIYHSGNYGGRIGCILNPEMVYARSSSPEDQKAAYMYDLFYN
                     RVFFDPMVKGYYTEEFLELCRKQSIYFDPATEDLETVQHHTVDFLGINQYYPKRVRAP
                     RYQWNEETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLIT
                     ENGMGREHEEQYMDEKGIVQDDYRIEFIGQHLRYLIKAAEEGANCEGYMLRAFTDCVS
                     PMNAFKNRYGLVRIELNDER"
BASE COUNT          204 a          156 c          116 g          238 t
ORIGIN      
        1 cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt
       61 gaaacacagt cagtgaatgc ccgtagcata taaccttcgc aatttgcacc ttcttctgct
      121 gctttgatca aataacgcaa atgctgtccg ataaattcga tacgatagtc atcttgcacg
      181 attcctttct catccatgta ctgttcttca tgttctcttc ccataccatt ttctgttatc
      241 agccaaggga tattatgata attttctttt agatacatgg ctatatcgta catgattttt
      301 ggatagatct cccaccctct agaattgttc attttctttc caggtaaatc aaagacttca
      361 taatatcgtt ctggatggaa aggtgtctct tcattccatt gataacgagg agcacgaacg
      421 cgtttagggt aatattgatt gatcccaagg aaatctactg tatgatgctg aaccgtttct
      481 aaatcctctg ttgcaggatc gaaataaatc gattgttttc gacacaactc taggaactct
      541 tctgtgtagt acccttttac catcggatca aaaaatactc gattataaaa cagatcatac
      601 atataagcag ctttttggtc ttctggagaa ctggaacgag catagaccat ctcaggattt
      661 aaaatacagc caatccttcc gccataattg ccactatggt aaatttctac agct
//
LOCUS       DACCVU010000312          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.340, whole genome shotgun sequence.
ACCESSION   DACCVU010000312 DACCVU010000000
VERSION     DACCVU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.340"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            63..671
                     /locus_tag="IVW29_003167"
     CDS             63..671
                     /locus_tag="IVW29_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7840550.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          266 a          139 c          132 g          175 t
ORIGIN      
        1 ttctaaatga taaagttaat agcgtcgaaa gaataaaaaa cggagaaaaa aaggagttta
       61 atatgaattc atttaagaaa attgttttag gtacaacttt tgctgctggt gctactgcaa
      121 tgtttgtagg aactacaaat gctcacgctg atgaagttta tacagtaaaa tcaggtgatt
      181 cattatctaa gatctcacaa aaatttgcag gagacaactc aatgatcgat gcaattgctg
      241 aaaaaaattc gatcgcaaac atcaaccgca tttatgttgg tgaacaatta acaatcccaa
      301 cttcaaatga ttcttcagca acaacagaaa acaaaacaac agaaaataca gcatctacaa
      361 ctgaaacagc aacgcaagaa catacatacg ttgcacctgt tgaaacagtt gaagtagctc
      421 ctgctgcccc agctgctgca acagcgccaa cttcaagctc tgcaaaagaa tggatcgcac
      481 aaaaagaatc aggcggttca tacacagcta ctaacggacg ttacatcggc cgttatcaat
      541 tagatgcttc atacttaaac ggcgactatt cagctgccaa tcaagaacgt gtagcagaac
      601 aatatgtaac atctcgttat ggttcatggg atgctgctaa aacattctgg ttagcaaacg
      661 gctggtacta agccgataaa aggaaaatga tgaaaccgag aaacttgttt at
//
LOCUS       DACCVU010000313          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.343, whole genome shotgun sequence.
ACCESSION   DACCVU010000313 DACCVU010000000
VERSION     DACCVU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.343"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            132..344
                     /locus_tag="IVW29_003168"
     CDS             132..344
                     /locus_tag="IVW29_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840551.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>699
                     /locus_tag="IVW29_003169"
     CDS             506..>699
                     /locus_tag="IVW29_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840552.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDI"
BASE COUNT          249 a          104 c          122 g          224 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatt
//
LOCUS       DACCVU010000314          694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.344, whole genome shotgun sequence.
ACCESSION   DACCVU010000314 DACCVU010000000
VERSION     DACCVU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.344"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            73..690
                     /locus_tag="IVW29_003170"
     CDS             73..690
                     /locus_tag="IVW29_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840553.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          250 a           96 c          136 g          212 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atag
//
LOCUS       DACCVU010000315          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.345, whole genome shotgun sequence.
ACCESSION   DACCVU010000315 DACCVU010000000
VERSION     DACCVU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.345"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(21..638)
                     /locus_tag="IVW29_003171"
     CDS             complement(21..638)
                     /locus_tag="IVW29_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7840554.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          253 a          128 c           70 g          241 t
ORIGIN      
        1 ctttattatc aatccgagtc tcatctttca ccatctcttt ttgtaatcaa aattagttta
       61 taaaggatta cacccaatat accgcccaac gtattatata caaaatccga aatttgaaac
      121 gttcctagcc tcaaaaaaag ttgtaaaaat tcaattattg ctgaaaaata aaatgcaatt
      181 tttcctccat atactattga ggaccatatt ttgggtttct ttatattttc atatttaata
      241 gacattacca tataaacata tggaataaaa agaataatat tttctaaaca cgctgtagat
      301 aactcttttg ttccagttgt tgtattatac ttataaatcc accagtctcc cataacatca
      361 gataaaggat ttaaccaaat tgttcgattg attaatgttg taaataaaat catcgcagta
      421 tagaatatca aagcacaaac ttttctatat tttctatcct tctttaaatg aagataaagt
      481 tccattagta tacgtttata gccctcacct gcagcaatgc catctgttgc atacataaaa
      541 tagaccagtc ctaaaattgt caataaaaca gcaaacccaa agggttcata taatttatat
      601 aatatatctg taataatttc tgcaatcact attatcataa ttatttctcc aatatcaagt
      661 atacacttat cgcttccaat tacacatgat ca
//
LOCUS       DACCVU010000316          685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.346, whole genome shotgun sequence.
ACCESSION   DACCVU010000316 DACCVU010000000
VERSION     DACCVU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.346"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..>685
                     /locus_tag="IVW29_003172"
     CDS             51..>685
                     /locus_tag="IVW29_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAP7840555.1"
                     /translation="MSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQN
                     CSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYV
                     RLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSV
                     LGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT          250 a          106 c          145 g          184 t
ORIGIN      
        1 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
       61 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
      181 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
      241 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
      301 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
      361 tttatggcgg aatcctgcat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
      421 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
      481 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
      541 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
      601 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
      661 acagtgaccc gcttctacaa tatta
//
LOCUS       DACCVU010000317          673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.348, whole genome shotgun sequence.
ACCESSION   DACCVU010000317 DACCVU010000000
VERSION     DACCVU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.348"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          248 a           97 c          163 g          165 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctc
//
LOCUS       DACCVU010000318          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.349, whole genome shotgun sequence.
ACCESSION   DACCVU010000318 DACCVU010000000
VERSION     DACCVU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.349"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            103..402
                     /locus_tag="IVW29_003173"
     CDS             103..402
                     /locus_tag="IVW29_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840556.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          236 a           86 c          109 g          221 t
ORIGIN      
        1 tgtaactgca aaaaaagaaa cagtattctc tgaaggcaaa ataaaaactc gatatatatt
       61 agaggtagag actaagttta aaaatagatc ggagtaatgg ttatgctttt taatgagacg
      121 caattatggt ttaaatttga tccttcgaat agatttgtca aagattttta taaggtgtgg
      181 gattcagaag ttttcttttt agcaatcgaa gatagcttat taattaatct ctactattct
      241 aataagaact actttaaaat ccctgctgcg aaaactagaa tgaagaaaga cgtatatttt
      301 ttgtttgata tcgtgactga tgtgctagac gctcgaagcg atcatcggcg ttatgactat
      361 ataaagtata ctttcgttga tccagaaaga tacaaagatt aaagtaggct acctaaaaag
      421 gtagcccgga acggatttta tcaccatact tatgaaagga gatatttttt aagttagtat
      481 taagattgtg taatatgatg atatctatat tttatagtat cagtgctata aaatcaaaaa
      541 taagtcacta attaactacc actccaatta taagcctttt ttctcacttt ttttcaaaaa
      601 tatggtatgc tttttaatgg cttcaaatat aaaagagttt aaagcgtaac ac
//
LOCUS       DACCVU010000319          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.350, whole genome shotgun sequence.
ACCESSION   DACCVU010000319 DACCVU010000000
VERSION     DACCVU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.350"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..364
                     /locus_tag="IVW29_003174"
     CDS             <1..364
                     /locus_tag="IVW29_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7840557.1"
                     /translation="VEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVN
                     SVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVT
                     YEQVLSIFNSYLLFNGRK"
BASE COUNT          218 a          111 c          103 g          200 t
ORIGIN      
        1 tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt
       61 gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa
      121 accaaaagta aacagtgtct taataaaact tacccgccat accacagatg ttccagataa
      181 atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact
      241 gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac
      301 cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa
      361 ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag
      421 ataaattatt aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa
      481 ttagaaaaaa acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc
      541 gctcgggacc ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg
      601 taactactct tttccttttt taaggtttaa at
//
LOCUS       DACCVU010000320          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.352, whole genome shotgun sequence.
ACCESSION   DACCVU010000320 DACCVU010000000
VERSION     DACCVU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.352"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..122)
                     /locus_tag="IVW29_003175"
     CDS             complement(<1..122)
                     /locus_tag="IVW29_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840558.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT          219 a           96 c          103 g          192 t
ORIGIN      
        1 acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac cggcttatta
       61 tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg tttgtaggtc
      121 atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt gtaccataaa
      181 tgccttttta tttttctaac ttaattttac aattcgcgat atattaaaca acgttgtcgc
      241 gcttcattga aacattttat gaaaattcca aaaaagagtt gacacatgtt tttagacgtg
      301 ataagataac taagtcgtta aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac
      361 ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga
      421 tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct
      481 gatatgatat aaaagttgct acggcagcca aaaacgaatc gggaaacact cccgatgaag
      541 tagatctttg aaaactgaac aaagtaagac aaaccaaatg tgtagggcgt cttgattcaa
      601 ttcaagacaa
//
LOCUS       DACCVU010000321          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.353, whole genome shotgun sequence.
ACCESSION   DACCVU010000321 DACCVU010000000
VERSION     DACCVU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.353"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..580
                     /locus_tag="IVW29_003176"
     CDS             <1..580
                     /locus_tag="IVW29_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7840559.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DACCVU010000322          586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.354, whole genome shotgun sequence.
ACCESSION   DACCVU010000322 DACCVU010000000
VERSION     DACCVU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.354"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            2..>586
                     /locus_tag="IVW29_003177"
     rRNA            2..>586
                     /locus_tag="IVW29_003177"
                     /product="23S ribosomal RNA"
BASE COUNT          186 a          119 c          167 g          114 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtcca
//
LOCUS       DACCVU010000323          571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.355, whole genome shotgun sequence.
ACCESSION   DACCVU010000323 DACCVU010000000
VERSION     DACCVU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.355"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            36..>571
                     /locus_tag="IVW29_003178"
     CDS             36..>571
                     /locus_tag="IVW29_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7840560.1"
                     /translation="MKLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTD
                     VPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMYADDPMTARATPKQAHDIV
                     VKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEI
                     QPTLERIEQKMVLNKHQET"
BASE COUNT          230 a          108 c          101 g          132 t
ORIGIN      
        1 cctttaaaca acagcaccct aattttgatg aactagtgaa actttatgaa aaagacgtag
       61 taaaacacgt taccccttat tctaatcgca tgacagaagc gatcataagc cgtttgagcg
      121 atcaagggta taatttggtg atcgaaggta caggacgaac aacagacgtt cctattcaaa
      181 ccgcaacaat gcttcaagcc aaaggttatg aaacaaaaat gtatgtcatg gcagtaccta
      241 aaatcaactc atatttagga acaattgaac gatatgaaac catgtatgca gatgatccaa
      301 tgacagccag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
      361 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctatat aacagagaag
      421 gagtaaaact ctattcaagt ttggaaacac cttctattag tccaaaagaa accttagaaa
      481 aagaattgaa tcgtaaagta tcagggaaag aaattcaacc gactttagaa cgaatagagc
      541 aaaaaatggt tctaaataaa caccaagaga c
//
LOCUS       DACCVU010000324          552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.356, whole genome shotgun sequence.
ACCESSION   DACCVU010000324 DACCVU010000000
VERSION     DACCVU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.356"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            68..>552
                     /locus_tag="IVW29_003179"
     CDS             68..>552
                     /locus_tag="IVW29_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7840561.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          207 a          100 c           90 g          155 t
ORIGIN      
        1 aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga
       61 cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt
      121 aaatgaatta ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt
      181 aacggctttt ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag
      241 aaacggttct tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc
      301 tagagatcgt aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga
      361 tacattggaa accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc
      421 tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac
      481 taaagttctg actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt
      541 cgctattttt at
//
LOCUS       DACCVU010000325          550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.358, whole genome shotgun sequence.
ACCESSION   DACCVU010000325 DACCVU010000000
VERSION     DACCVU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.358"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            12..>550
                     /locus_tag="IVW29_003180"
     CDS             12..>550
                     /locus_tag="IVW29_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7840562.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRK"
BASE COUNT          166 a           83 c           78 g          223 t
ORIGIN      
        1 catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt
       61 taagtttaga atctattttt agctccccta ttaattttga aacgtttaga tcatatggca
      121 tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt
      181 ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc attactctat
      241 ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg
      301 ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg
      361 gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg
      421 actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc
      481 atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt
      541 atcggaaaaa
//
LOCUS       DACCVU010000326          524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.363, whole genome shotgun sequence.
ACCESSION   DACCVU010000326 DACCVU010000000
VERSION     DACCVU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.363"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..376
                     /locus_tag="IVW29_003181"
     CDS             <1..376
                     /locus_tag="IVW29_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7840563.1"
                     /translation="SATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTKR
                     GDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVIK
                     IENQERKRQFKRSQQMNQEMER"
     gene            379..>524
                     /gene="ssb"
                     /locus_tag="IVW29_003182"
     CDS             379..>524
                     /gene="ssb"
                     /locus_tag="IVW29_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7840564.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADF"
BASE COUNT          207 a           85 c          100 g          132 t
ORIGIN      
        1 aagtgctacg ttcagctttg gcacagataa ttcgagagca aaagattact acgacttatt
       61 taccattccg aaactagaaa atatcgatac gaaagagtta tacaaaagtg ttcgaaatac
      121 atttacaaaa cgtggggata caaaaccgtt gtccttttat tatgaaagag aaatggataa
      181 gatcagagga aatgactatt tggaaaagca gtgggaaaaa tatcgctcag ttaacacgtt
      241 tgcgaattcc atttcattta cagatacagt caacgaaatt gatcacctaa tgaaaagtgt
      301 gatcaaaatt gaaaatcaag aaagaaaacg gcagtttaaa agatcacaac aaatgaatca
      361 agaaatggag agataaagat gatcaataat gtaactttag taggacgctt aaccaaagat
      421 ccagatttac gttataccgc gagtggtact gcagtagcga cttttaccct agctgtcaac
      481 cgaaatttca cgaatcaaaa cggcaatcga gaagctgatt ttat
//
LOCUS       DACCVU010000327          507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.366, whole genome shotgun sequence.
ACCESSION   DACCVU010000327 DACCVU010000000
VERSION     DACCVU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.366"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..342)
                     /gene="tnpB"
                     /locus_tag="IVW29_003183"
     CDS             complement(<1..342)
                     /gene="tnpB"
                     /locus_tag="IVW29_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7840565.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKK"
BASE COUNT          146 a          109 c           89 g          163 t
ORIGIN      
        1 tttttttcct ggcaaaatag ctcgaggttg atcgatactt aatccttcta acaaccatcg
       61 aacctgttgg ttcgttaagg ttcgaacttc gtctttcgtc cgaggccatt ttaacttgcc
      121 gttttctatc cgcttataac agagaagaaa accatcaccg tcccaataga gaagtttgaa
      181 acggtcttga cggttcccgc aaaataaaaa aacggcatct tcgtaaagct cgagttgata
      241 ctcttgttga ataagactgg caagaccatc aattcctttt cgcaaatctg tttttccgca
      301 cacaatataa atatgtttgg tttgaagtaa atcataaagc attggacaac agttctttca
      361 aaatagcttg aagaatataa ttgttaatac cgttatagat caaaacttca gaaccattct
      421 ctcttttgat gcgcatcgct aacttggcag ccgcgggttt acctttttct ggtgcactct
      481 tttcgtttcc taattggaca ggaacaa
//
LOCUS       DACCVU010000328          495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.371, whole genome shotgun sequence.
ACCESSION   DACCVU010000328 DACCVU010000000
VERSION     DACCVU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.371"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..269
                     /locus_tag="IVW29_003184"
                     /pseudo
     CDS             1..269
                     /locus_tag="IVW29_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DACCVU010000329          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.373, whole genome shotgun sequence.
ACCESSION   DACCVU010000329 DACCVU010000000
VERSION     DACCVU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.373"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="IVW29_003185"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="IVW29_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7840566.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          141 a          102 c           85 g          165 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatctccc atc
//
LOCUS       DACCVU010000330          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.375, whole genome shotgun sequence.
ACCESSION   DACCVU010000330 DACCVU010000000
VERSION     DACCVU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.375"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..454
                     /locus_tag="IVW29_003186"
     CDS             <1..454
                     /locus_tag="IVW29_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP7840567.1"
                     /translation="ETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRL
                     VKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNK
                     FYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          166 a           90 c           97 g          131 t
ORIGIN      
        1 tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca tcgatgcaga
       61 tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag cctatgcttt
      121 tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag ataaagcccc
      181 ctctattaca agtgccttta agaaactaaa agaatacggc ttttatcaag ggacagaaca
      241 tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag taaagagacg
      301 caataaattc tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat
      361 tcgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
      421 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
      481 tctt
//
LOCUS       DACCVU010000331          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.377, whole genome shotgun sequence.
ACCESSION   DACCVU010000331 DACCVU010000000
VERSION     DACCVU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.377"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            47..319
                     /locus_tag="IVW29_003187"
     CDS             47..319
                     /locus_tag="IVW29_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7840568.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            321..>479
                     /locus_tag="IVW29_003188"
     CDS             321..>479
                     /locus_tag="IVW29_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840569.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAI"
BASE COUNT          185 a           64 c           88 g          142 t
ORIGIN      
        1 atacggaaat atttacctga caaactttaa aaggagtgaa aatgaaatgg cagttacgta
       61 tgaaaaaaca tttgaaatag agatcattaa cgaattatcg gcaagcgttt ataatcgagt
      121 attaaactat gttttgaacc atgaattaaa taaaaatgac tctcaattat tggaagtcaa
      181 tttattaaac caattaaagc ttgcaaaacg tgtaaatctt tttgattatt ctttagaaga
      241 attacaagcc gttcatgagt attggcggtc aatgaatcgt tactcaaaac aagttttgaa
      301 taaagagaaa gtggcttaat atggcaaata tagtcaattt tactgacaaa caatttgaga
      361 atcgcttaaa tgataattta gaagaattga ttcaaggaaa aaaagcggtt gaatcgccaa
      421 ccgctttttt acttggtggg caaccagggt cagggaaaac cagtttgcga tcagcaatt
//
LOCUS       DACCVU010000332          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.378, whole genome shotgun sequence.
ACCESSION   DACCVU010000332 DACCVU010000000
VERSION     DACCVU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.378"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>468
                     /locus_tag="IVW29_003189"
     CDS             <1..>468
                     /locus_tag="IVW29_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7840570.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACCVU010000333          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.379, whole genome shotgun sequence.
ACCESSION   DACCVU010000333 DACCVU010000000
VERSION     DACCVU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.379"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..426)
                     /locus_tag="IVW29_003190"
     CDS             complement(<1..426)
                     /locus_tag="IVW29_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7840571.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQ"
BASE COUNT          138 a           97 c           70 g          162 t
ORIGIN      
        1 ctgataaacc gttgataaat caattccatt ttggtcaatc ttattgataa aaaagattgt
       61 gggaatcccc attttcctaa gtgcatgaaa taatatacga gtttgtgctt gtacgccatc
      121 ttttgcagaa atcagtagaa ttgccccatc taaaactgat aatgaacgat atacttctgc
      181 taagaaatcc atatgtcctg gcgtgtctat gatgttcacc ttcgtatttt cccactgaaa
      241 agaggttatt cctgtctgaa ttgtaattcc tctctgacgt tctaaaagcg tattatccgt
      301 cctcgttgta cctttgtcca cgcttcctaa ttctgtaatc gctccactgt tatataataa
      361 gctttctgtt aaggtagttt ttcctgcatc aacatgagct aaaactccaa tattaataat
      421 tttcatgtga ttttcctcca ttcaaaaacc caaaagggca taaaaat
//
LOCUS       DACCVU010000334          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.380, whole genome shotgun sequence.
ACCESSION   DACCVU010000334 DACCVU010000000
VERSION     DACCVU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.380"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..466
                     /locus_tag="IVW29_003191"
                     /pseudo
     CDS             1..466
                     /locus_tag="IVW29_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          191 a           83 c           83 g          109 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccatta
//
LOCUS       DACCVU010000335          465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.381, whole genome shotgun sequence.
ACCESSION   DACCVU010000335 DACCVU010000000
VERSION     DACCVU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.381"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>465)
                     /locus_tag="IVW29_003192"
     CDS             complement(<1..>465)
                     /locus_tag="IVW29_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7840572.1"
                     /translation="NSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAF
                     KIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNF
                     KIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIH"
BASE COUNT          153 a           64 c           64 g          184 t
ORIGIN      
        1 atgaatattt gctaatttcg aagtttgtaa agtagtttct tcaatatttt tcaattcaag
       61 tggtaaatct ggtcctccaa taacattttt atttttgaac gaggaattaa aaaaaagtgt
      121 atgaaaaaat ttttcatctg gggcaaacat acctatttta aaattcaggt agtcttgttg
      181 attttgttca tacatttgta ttaattctgt taggcattct ttcgacaaag cccaccattg
      241 agaaccatga tacaaatcaa atttatggtg tctaattttt gggggaagtt taatcatcct
      301 cagacaacga ttaattatag ctctaaaaat tttaaatgca gttgtgttat tcctattaat
      361 tctaggataa tcatgttttt gatatacatc tatatgttta taataaaatt ctttatgttt
      421 aatttgatct agatctattc cacgaataaa ctccactgaa ctatt
//
LOCUS       DACCVU010000336          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.382, whole genome shotgun sequence.
ACCESSION   DACCVU010000336 DACCVU010000000
VERSION     DACCVU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.382"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..430)
                     /locus_tag="IVW29_003193"
     CDS             complement(<1..430)
                     /locus_tag="IVW29_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7840573.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCVU010000337          456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.384, whole genome shotgun sequence.
ACCESSION   DACCVU010000337 DACCVU010000000
VERSION     DACCVU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.384"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..>456
                     /locus_tag="IVW29_003194"
     CDS             5..>456
                     /locus_tag="IVW29_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7840574.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT          159 a           69 c          103 g          125 t
ORIGIN      
        1 gtaaatggtc atgaatgcta tttcggcgat gaaaaaaaca aaacagcagc tacatgaagc
       61 ggggtttaag ctgacaccgc aaagagaagc tactctgctt gtgcttttgg aaaatgagaa
      121 agagcattta tctgcagaag aaatcttctt tttagtgaaa aaaaagagtc cggaaattgg
      181 acttgctact gtttatcgaa cccttgaaat cttgacagaa ttgaaaattg tcgataaagt
      241 aagttttaat gatggattag cccgttatga tttgagaaaa gaaggcgcca aacatttcca
      301 tcatcattta ctatgcttga actgcggcac gatcgaggaa gtagaagagg atcttttact
      361 tgaagtggag caagtagtag aagaacgtta tcatttttta gtaaaagatc atcggctaac
      421 gttccatgga atctgtcagg aatgctatga aaaaga
//
LOCUS       DACCVU010000338          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.385, whole genome shotgun sequence.
ACCESSION   DACCVU010000338 DACCVU010000000
VERSION     DACCVU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.385"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          145 a           87 c           52 g          167 t
ORIGIN      
        1 aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc
       61 tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat
      121 atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc
      181 cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt
      241 cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa
      301 actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat
      361 cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac
      421 actactattc agcccattca acgaccaatg a
//
LOCUS       DACCVU010000339          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.392, whole genome shotgun sequence.
ACCESSION   DACCVU010000339 DACCVU010000000
VERSION     DACCVU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.392"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>430
                     /locus_tag="IVW29_003195"
     CDS             <1..>430
                     /locus_tag="IVW29_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP7840575.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACCVU010000340          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.393, whole genome shotgun sequence.
ACCESSION   DACCVU010000340 DACCVU010000000
VERSION     DACCVU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.393"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..171)
                     /locus_tag="IVW29_003196"
     CDS             complement(<1..171)
                     /locus_tag="IVW29_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7840576.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISI"
     gene            complement(190..345)
                     /locus_tag="IVW29_003197"
                     /pseudo
     CDS             complement(190..345)
                     /locus_tag="IVW29_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          143 a           76 c           46 g          158 t
ORIGIN      
        1 tattgatatt gaagaattag aactttcata taaagccttt tctatttcat tgcttgattt
       61 cattattgtt aaacaagaat acaagcaaga aagaactata gttaatatga taaaaattat
      121 taaagttcta tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac
      181 tactcctctc tattttctta ctccataact aattgcatcc actggacaag catttttaca
      241 tcgtccacat ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg
      301 gcaaactttt ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac
      361 tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc
      421 atg
//
LOCUS       DACCVU010000341          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.395, whole genome shotgun sequence.
ACCESSION   DACCVU010000341 DACCVU010000000
VERSION     DACCVU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.395"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..339)
                     /locus_tag="IVW29_003198"
     CDS             complement(<1..339)
                     /locus_tag="IVW29_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7840577.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACCVU010000342          411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.399, whole genome shotgun sequence.
ACCESSION   DACCVU010000342 DACCVU010000000
VERSION     DACCVU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.399"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>411
                     /locus_tag="IVW29_003199"
     rRNA            <1..>411
                     /locus_tag="IVW29_003199"
                     /product="16S ribosomal RNA"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DACCVU010000343          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.400, whole genome shotgun sequence.
ACCESSION   DACCVU010000343 DACCVU010000000
VERSION     DACCVU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.400"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>409
                     /locus_tag="IVW29_003200"
     CDS             <1..>409
                     /locus_tag="IVW29_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840578.1"
                     /translation="LPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDD
                     KSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTV
                     VVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          167 a           61 c           93 g           88 t
ORIGIN      
        1 gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca gatgcgaaat
       61 ggccagagga agcaactcaa ccaattacag cagatgatac agaatttgta tcaagtgcaa
      121 caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa aaagtgacta
      181 cagaattgaa taaggaacca gacgcatttg agggaattaa gaacaaagaa gatttaccaa
      241 aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga aataaaaaag
      301 ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta gatgttacag
      361 tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DACCVU010000344          407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.401, whole genome shotgun sequence.
ACCESSION   DACCVU010000344 DACCVU010000000
VERSION     DACCVU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.401"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          146 a           50 c           70 g          141 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaa
//
LOCUS       DACCVU010000345          397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.407, whole genome shotgun sequence.
ACCESSION   DACCVU010000345 DACCVU010000000
VERSION     DACCVU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.407"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..378
                     /locus_tag="IVW29_003201"
     CDS             <1..378
                     /locus_tag="IVW29_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7840579.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          150 a           71 c           63 g          113 t
ORIGIN      
        1 gaaatctgtg atgactttaa ggctgtttat caagctaact caaaagaaga agcgaatacc
       61 ttcttatccg gcatgattga gaaatggaag aaaaactatc ctaaagtgac gcagtcactc
      121 atagaaaacc aagacttatt aactttttat gattttccac ctagcattcg tagaaccatt
      181 tactcaacca atctaatcga gtctttcaat aagcaaatta aaagatacag ccgtagaaaa
      241 gagcagtttc aaaatgaaga atcactagaa cgctttctag tcagcatttt tgatacatac
      301 aatcaaaaat ttctaaacag aagccataaa ggttttcaac aggtaaccga tacattagtt
      361 tcaatgttta ctgagtaact aattattttg caggagg
//
LOCUS       DACCVU010000346          392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.408, whole genome shotgun sequence.
ACCESSION   DACCVU010000346 DACCVU010000000
VERSION     DACCVU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.408"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          156 a           45 c           58 g          133 t
ORIGIN      
        1 ttgtcacata attttgcaat caataaatgt tgcgcaataa gtataagaat tccattttct
       61 aaatttgaaa ggtgatccat aatttcatgt tcaagtagtt tattaattgt ccacttgtcg
      121 cttcatctaa aaattttata ggttttagga atgctattcg cttattttag gaagatttta
      181 actgtttgct atagatatat atcatactag tatcgtaata aaagtactct aaaagaagaa
      241 ttttcaaata cgcaaaatta agtacgaaag agttgaaaca aatttggaga aaaatgagca
      301 tagaatgtct gatgagttaa atagacctaa aaattatcta aaagtggagc aaaattagga
      361 attacaaaca aaacactaaa ttagtttata ta
//
LOCUS       DACCVU010000347          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.409, whole genome shotgun sequence.
ACCESSION   DACCVU010000347 DACCVU010000000
VERSION     DACCVU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.409"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          157 a           36 c           62 g          124 t
ORIGIN      
        1 aataaaatgc tgtaatattc gtgtttaaaa ataataaata aaaataggga attttctatt
       61 gcaattttat aaagagattg ctatattatt atttgttcta aatgattcct ttaaggtgtc
      121 actaaaaatt ataaaaaata gtgttgacgc acttttgatg tcatgttata ataacgaagt
      181 cgtttggatg agatagctaa taaagcttga ttttatcgaa aaaaacttcg aaaaagttat
      241 tgacaaataa caataaagat ggtatgataa tcaagtcgct gctaatgagt ggcaaagacg
      301 tttagaaaaa ataaacaaag aagttgttga cattctcttc tgaatctgat aagattaaag
      361 agttgttaaa acaaaaaga
//
LOCUS       DACCVU010000348          364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.413, whole genome shotgun sequence.
ACCESSION   DACCVU010000348 DACCVU010000000
VERSION     DACCVU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.413"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          130 a           54 c           48 g          132 t
ORIGIN      
        1 tcttcattct ggtttttgtt gccggaattt taaagtagtt cttattagag taatagagat
       61 tgattaataa gctatcttcg attgctaaaa agaaaacttc tgaatcccac accttataaa
      121 aatctttgac aaatctattc gaaggatcaa atttaaacca taattgtgtt tttccttcca
      181 tcaacataat gttcacctca aaaagagtat acgaactaat gttctttttg taaagtagga
      241 atatcattga taatatggat ggaacatata tattaaaaag tgttcttttt gaatgctatt
      301 ttgaatgcca ttttcattca tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct
      361 tata
//
LOCUS       DACCVU010000349          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.430, whole genome shotgun sequence.
ACCESSION   DACCVU010000349 DACCVU010000000
VERSION     DACCVU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.430"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>321)
                     /locus_tag="IVW29_003202"
     rRNA            complement(<1..>321)
                     /locus_tag="IVW29_003202"
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           96 c           74 g           88 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat g
//
LOCUS       DACCVU010000350          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.431, whole genome shotgun sequence.
ACCESSION   DACCVU010000350 DACCVU010000000
VERSION     DACCVU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.431"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>321
                     /locus_tag="IVW29_003203"
     CDS             <1..>321
                     /locus_tag="IVW29_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7840580.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DACCVU010000351          314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.434, whole genome shotgun sequence.
ACCESSION   DACCVU010000351 DACCVU010000000
VERSION     DACCVU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.434"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>314
                     /locus_tag="IVW29_003204"
     rRNA            <1..>314
                     /locus_tag="IVW29_003204"
                     /product="23S ribosomal RNA"
BASE COUNT           95 a           58 c           92 g           69 t
ORIGIN      
        1 acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaaacg aacttggaga
       61 tagctggttc tctccgaaat agctttaggg ctagcctcgg aattgagaat gatggaggta
      121 gagcactgtt tggactaggg gcccatctcg ggttaccgaa ttcagataaa ctccgaatgc
      181 cattcattca tatccgggag tcagactgtg agtgataaga tccatagtcg aaagggaaac
      241 agcccagacc accagctaag gtcccaaaat atatgttaag tggaaaagga tgtggggttg
      301 cacagacaac tagg
//
LOCUS       DACCVU010000352          312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.435, whole genome shotgun sequence.
ACCESSION   DACCVU010000352 DACCVU010000000
VERSION     DACCVU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.435"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          125 a           42 c           47 g           98 t
ORIGIN      
        1 tataaaagag tttaaagcgt aatacactta tggggaagtg gtttggggtg cgctttaaac
       61 tcttctttat tattatctca cagcatgctt aaaaggtcta tggattaagt tatgtaaaaa
      121 agtgcctctt taaacgtcct aaaatccaaa caaggatagc ataatataca ctatcgttga
      181 tccagaaaac aggtaaataa attttgcaaa agcaataagt taaaaacatt acaaaaaaga
      241 caaggatatc acatcaagaa aaaatgtttt gaaaatatct ttgccttttt gtattatatc
      301 tatattaaat aa
//
LOCUS       DACCVU010000353          310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.denovo.436, whole genome shotgun sequence.
ACCESSION   DACCVU010000353 DACCVU010000000
VERSION     DACCVU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.denovo.436"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..261
                     /locus_tag="IVW29_003205"
     CDS             <1..261
                     /locus_tag="IVW29_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7840581.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          114 a           60 c           54 g           82 t
ORIGIN      
        1 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
       61 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
      121 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
      181 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
      241 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
      301 tggacttgac
//
LOCUS       DACCVU010000354        10486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.108, whole genome shotgun sequence.
ACCESSION   DACCVU010000354 DACCVU010000000
VERSION     DACCVU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.108"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..193
                     /locus_tag="IVW29_003206"
     CDS             <1..193
                     /locus_tag="IVW29_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840582.1"
                     /translation="VAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRAQELLTYYL
                     TLEPGDMEALSLLDDLTER"
     gene            199..780
                     /locus_tag="IVW29_003207"
     CDS             199..780
                     /locus_tag="IVW29_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7840583.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(862..1737)
                     /locus_tag="IVW29_003208"
     CDS             complement(862..1737)
                     /locus_tag="IVW29_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7840584.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1885..2217
                     /locus_tag="IVW29_003209"
     CDS             1885..2217
                     /locus_tag="IVW29_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7840585.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2329..3537
                     /locus_tag="IVW29_003210"
     CDS             2329..3537
                     /locus_tag="IVW29_003210"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7840586.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            3691..4146
                     /locus_tag="IVW29_003211"
     CDS             3691..4146
                     /locus_tag="IVW29_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840587.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            4508..4783
                     /locus_tag="IVW29_003212"
     CDS             4508..4783
                     /locus_tag="IVW29_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840588.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            4796..6874
                     /locus_tag="IVW29_003213"
     CDS             4796..6874
                     /locus_tag="IVW29_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7840589.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            7034..8920
                     /locus_tag="IVW29_003214"
     CDS             7034..8920
                     /locus_tag="IVW29_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7840590.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8932..9879
                     /locus_tag="IVW29_003215"
     CDS             8932..9879
                     /locus_tag="IVW29_003215"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7840591.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9899..10426
                     /locus_tag="IVW29_003216"
     CDS             9899..10426
                     /locus_tag="IVW29_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7840592.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT         3514 a         1750 c         2252 g         2970 t
ORIGIN      
        1 cgttgcagct gtacactatg aacaggctta tcatgaacta tcacatgaac cagatttctt
       61 gaaagagtat gcctttttct taagagaaga agggcagctg aaaagagcgc aagaattatt
      121 gacttattat ttaacacttg aaccaggaga tatggaggca ctgtcattat tagatgacct
      181 aacagagagg tgagaaaaat gctaattgat gtacgggaaa agaagaattt tttaacgtgg
      241 atggtcaatc atgtatcttt tagccgaaga gaagtttttt ggatcttgaa ttatcttgcc
      301 aatcatgaag cgattttaag caatgtccac tttgttgaag gggcgaattt tgcggatcga
      361 ggactgcaga tcagtgatct atctgttgaa ggggaaccca tgaaattgtt tttgcaggga
      421 aaagaattta cggacacaga ccagattttt catgaaatcc gtttgaattg gaaaaagcct
      481 ttatatgtgg aatgtatatt cgaaaatgct tggcagacaa gagaatatct atctatctta
      541 gaagacaatc cattttcttc ttgggattct tcagtcagtg aagaagatac tgaattaatt
      601 gaacgatatt ttcaagaaaa agaagaagag gcaaaactaa aaatgcttta tgctcaaatc
      661 gatcaagcac tagaagatgg agatagggat gcttttttga aattatctga tgaagttaac
      721 cgaaagatcc ttttgcagac acaaaagaaa agtaaccggg taagtgaaca taacaattag
      781 ttaaaaatag tatagtacta aaaaagacaa aggtcagctg gatgtggaat ctttcctcat
      841 agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg gcttcaaata
      901 agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag ctttttcgtc
      961 aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga ccatatagat
     1021 gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca cacctctttg
     1081 cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga caagtagacc
     1141 ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa catacgaaac
     1201 aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa gtaagacgat
     1261 gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt ctaaaatccg
     1321 agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc cgcttcctac
     1381 acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta aattgatttc
     1441 tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac ctagaacagt
     1501 ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg ggatgttgat
     1561 taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca aggtgatccc
     1621 tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac caaatgcata
     1681 aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt tgaccatatg
     1741 aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc cttcctaaat
     1801 gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt aaagttttgg
     1861 taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag tatgcagcag
     1921 gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt agctcagatg
     1981 gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac ttatggagaa
     2041 aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg tgatgtttta
     2101 ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt ttttgaaaaa
     2161 aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga cgagtaaaca
     2221 agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag tcaccatgca
     2281 actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat gaaattaaaa
     2341 caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga gaatgcagga
     2401 tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca gccaatccat
     2461 gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt tccaaaaacg
     2521 atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga acaatatgag
     2581 atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga tcatgtcgaa
     2641 ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa tgcgtttgct
     2701 ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct agaagaacag
     2761 atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt acgaatgatg
     2821 cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac cttctacgcc
     2881 atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa tgtggaattc
     2941 atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat tgaaacagag
     3001 tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg ttttgctgaa
     3061 ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat cagccaactg
     3121 gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc taaccaaatg
     3181 attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg agctttggaa
     3241 ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga attgatgatc
     3301 tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg tttacctatc
     3361 aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac tggaaaaaaa
     3421 cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt agaacaaaga
     3481 ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt gatttaaaaa
     3541 acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat acgtcattag
     3601 aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag gtagtacaag
     3661 aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc gctaaaattt
     3721 tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga tctttggatc
     3781 aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga aatccatccg
     3841 tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac acatgatatc
     3901 aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga agatatcgag
     3961 cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc atatctagat
     4021 aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga aaaaacaggt
     4081 aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg ggaatttttc
     4141 gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt attactactg
     4201 ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag atttttgtcg
     4261 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
     4321 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
     4381 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
     4441 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
     4501 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
     4561 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
     4621 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
     4681 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
     4741 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
     4801 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
     4861 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
     4921 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
     4981 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
     5041 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
     5101 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
     5161 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
     5221 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     5281 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     5341 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     5401 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     5461 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     5521 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     5581 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     5641 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     5701 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     5761 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     5821 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     5881 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     5941 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     6001 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     6061 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     6121 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     6181 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     6241 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     6301 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     6361 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     6421 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     6481 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     6541 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     6601 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     6661 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     6721 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     6781 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     6841 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     6901 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     6961 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     7021 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     7081 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     7141 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     7201 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     7261 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     7321 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     7381 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     7441 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     7501 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     7561 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     7621 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     7681 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     7741 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     7801 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     7861 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     7921 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     7981 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     8041 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     8101 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     8161 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     8221 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     8281 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     8341 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     8401 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     8461 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     8521 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     8581 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     8641 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     8701 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     8761 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     8821 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     8881 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     8941 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     9001 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     9061 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     9121 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     9181 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     9241 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     9301 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     9361 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     9421 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     9481 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     9541 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     9601 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     9661 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     9721 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     9781 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     9841 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     9901 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     9961 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
    10021 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
    10081 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
    10141 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
    10201 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
    10261 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
    10321 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
    10381 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
    10441 gactagctgt tatttagagc tgtcccatcc atagctttat tttttt
//
LOCUS       DACCVU010000355         5994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.151, whole genome shotgun sequence.
ACCESSION   DACCVU010000355 DACCVU010000000
VERSION     DACCVU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.151"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            295..1191
                     /locus_tag="IVW29_003217"
     CDS             295..1191
                     /locus_tag="IVW29_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7840593.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1341..2282)
                     /locus_tag="IVW29_003218"
     CDS             complement(1341..2282)
                     /locus_tag="IVW29_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7840594.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2408..3529
                     /locus_tag="IVW29_003219"
     CDS             2408..3529
                     /locus_tag="IVW29_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7840595.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3787..5289
                     /locus_tag="IVW29_003220"
     CDS             3787..5289
                     /locus_tag="IVW29_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7840596.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5356..5883
                     /locus_tag="IVW29_003221"
     CDS             5356..5883
                     /locus_tag="IVW29_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7840597.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1975 a         1053 c         1235 g         1731 t
ORIGIN      
        1 tgttttttca attttttatc ttccataaat gacctctttc ttttaaattc acaatcaaaa
       61 tttatattaa gaataaatag caatatttaa caatgactaa tgataacaaa ttttcagaca
      121 attacaacta ttcagaaaac ttttaggcga attttaatag aagaatgatt tatttcatca
      181 tattttcata caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta
      241 ctatttaaac aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac
      301 aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc
      361 tctttagatg tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga
      421 caacagttgg caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg
      481 gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc
      541 aaagcagaac acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt
      601 tatcattttg attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat
      661 tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg
      721 aataatggga gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt
      781 acgaccaact atttcattct tccacatcga gggaatgaac aacctggata tcttacttat
      841 gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt
      901 cagcaaggag tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg
      961 gataatcaag cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga
     1021 gaacgtgatt tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca
     1081 gagctattgg aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc
     1141 actatttatg agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac
     1201 attttgaaga gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt
     1261 gtgttttctt tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct
     1321 gattctcatc agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat
     1381 tgggtcaaaa aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg
     1441 gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata
     1501 gatgggtaat gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact
     1561 tcttcttgat ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt
     1621 gttcgaacca tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa
     1681 atcgtgcata aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga
     1741 acgttatggc tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt
     1801 gtaaatgctt cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat
     1861 gtgacttttc cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt
     1921 ttctttggca catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa
     1981 acataagtat tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg
     2041 gcttttgtta atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt
     2101 gaatagattg gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt
     2161 gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt
     2221 tgatgtagca tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc
     2281 atgttttttc tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa
     2341 atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg
     2401 gatacagatg gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga
     2461 actaaatcag gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc
     2521 gattgcagat ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg
     2581 gcgtaccatt gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac
     2641 aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct
     2701 acttgataaa ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact
     2761 tattaaagat gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg
     2821 gtgtagcgat ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac
     2881 agaaggaaat cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt
     2941 ttctaaagta aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg
     3001 agctgcttgg caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga
     3061 taaacaagca ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga
     3121 tattccagga agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa
     3181 gatggtttct ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca
     3241 agcagatgtc atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt
     3301 tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg
     3361 aagtagagac caagagctag aaggactttc tgattttctc ccaattgtct taagtattca
     3421 tttgggtcca ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa
     3481 aatcttagga cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc
     3541 attttcttta tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa
     3601 aaattaagtc gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag
     3661 tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc
     3721 aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga
     3781 tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca
     3841 ttattatttg aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga
     3901 cgaaacggtc gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa
     3961 gggcaagtaa tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa
     4021 cgtttaacct attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga
     4081 gagctccaat tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg
     4141 gggggagaga agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg
     4201 ttaatcgatg aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat
     4261 ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa
     4321 gtagtggacc atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc
     4381 tctatctatg aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa
     4441 ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt
     4501 tcccgagaag gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga
     4561 gtgaataaag gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc
     4621 gtgaatcgga tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat
     4681 atcgattctt tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa
     4741 gatcttcaat tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct
     4801 catcagcggg tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat
     4861 cttctggggg cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc
     4921 attagttatg caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag
     4981 aaaaaccaag tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga
     5041 gatgtttttc ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg
     5101 gctaaatctt tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg
     5161 gatgtcttca atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta
     5221 ctagtggaac atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag
     5281 agaatctaac ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac
     5341 gaaggagaga atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa
     5401 ggattagttt gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag
     5461 aaactagcag aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct
     5521 cttcaacgag caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc
     5581 gttgatgaac gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat
     5641 gagaactttc aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc
     5701 ttagatgtgt atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat
     5761 gtctatctgt tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg
     5821 atgcctaatg aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag
     5881 taattatgtt tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt
     5941 gcctttttta ggttatgaca attcttttga taatataaac tcaaccttta gaaa
//
LOCUS       DACCVU010000356         4106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.177, whole genome shotgun sequence.
ACCESSION   DACCVU010000356 DACCVU010000000
VERSION     DACCVU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.177"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003222"
     CDS             44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7840598.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2073..2123
                     /locus_tag="IVW29_003223"
                     /pseudo
     CDS             2073..2123
                     /locus_tag="IVW29_003223"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003224"
     CDS             2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7840599.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
BASE COUNT         1298 a          630 c          842 g         1336 t
ORIGIN      
        1 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
       61 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
      121 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      181 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      241 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      301 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      361 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      421 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      481 gaaactttct atggaaatta taatcaaaca gaaagtagag ctgcacccta atatgtgtgt
      541 gatgagctgt acggaacctg agcaatggga tgtggtaata gaaggaaatg atgacctttt
      601 agagaaatat atgtccggta aatcattaga agcattagaa ctcgaacaag aggaaatcag
      661 aagatttcag aattgctcct tgtaccctgt ttatcatgga agcgcaaaaa gcaacatagg
      721 gattgagcag cttatagaag tgataacgaa taaattttat tcatcaacat acagaaagaa
      781 gtctgaactt tgcggaaatg tcttcaaaat tgaatattcg gaagaaagac aacgtcttgc
      841 atatgtacgc ctttatggcg gaatcctgca tttgcgggat tcggttagaa tatcggaaaa
      901 ggaaaaaata aaaattacag aaatgtatac ttcaataaat ggtgaattat gtaaaattga
      961 taaggcttat tccggggaaa ttgttatttt gcaaaatgag tttttgaagc taaatagtgt
     1021 tcttggagat acaaagctat tgccacagag agagagaatt gaaaatccgc tccctctgct
     1081 gcaaacaact gttgaaccga gcaaacctca acaaagggaa atgttacttg atgcactttt
     1141 agaaatctcc gacagtgacc cgcttctaca atattatgtg gattctacga cacatgaaat
     1201 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1261 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1321 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1381 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1441 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
     1501 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt atggcttata
     1561 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
     1621 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
     1681 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
     1741 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
     1801 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
     1861 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
     1921 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
     1981 tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa
     2041 gtgtaaagga ggaattaatt atgaagtgaa atgaatgtaa cagggttcaa ttaaaagagg
     2101 gaagcgtatc attaacccta taaactacgt ctgccctcat tattggaggg tgaaatgtga
     2161 atacatccta ttcacaatcg aatttacgac acaaccaaat tttaatttgg ctttgcattt
     2221 tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct gatattgcaa
     2281 atgattttaa taaaccacct gcgagtacaa actgggtgaa cacagccttt atgttaacct
     2341 tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc aaaaggttac
     2401 tcctatttgg aattataata aattgtttcg ggtcggtaat tgggtttgtt ggccattctt
     2461 tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct gcatttccag
     2521 cactcgtaat ggttgtagtt gcgcgctata ttccaaagga aaataggggt aaagcatttg
     2581 gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt ggtggaatga
     2641 tagcccatta tattcattgg tcctatcttc tactcattcc tatgataaca attatcactg
     2701 ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat tttgatatca
     2761 aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca acatcatata
     2821 gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa catatcagga
     2881 aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt atgattggag
     2941 ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg gttccttata
     3001 tgatgaaaga tgttcaccag ctaagtactg ccgaaatcgg aagtgtaatt attttccctg
     3061 gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat agaagaggtc
     3121 ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta actgcttcct
     3181 ttcttttaga aacaacatca tggttcatga caattataat cgtatttgtt ttaggtgggc
     3241 tttcgttcac caaaacagtt atatcaacaa ttgtttcaag tagcttgaaa cagcaggaag
     3301 ctggtgctgg aatgagtttg cttaacttta ccagcttttt atcagaggga acaggtattg
     3361 caattgtagg tggtttatta tccataccct tacttgatca aaggttgtta cctatggaag
     3421 ttgatcagtc aacttatctg tatagtaatt tgttattact tttttcagga atcattgtca
     3481 ttagttggct ggttaccttg aatgtatata aacattctca aagggatttc taaatcgtta
     3541 agggatcaac tttgggagag agttcaaaat tgatcctttt tttataacag gaattcaaat
     3601 ctttttgttc cattaaaggg cgcgattgct gaataaaaga tacgagagac ctctcttgta
     3661 tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac aataaaaatt
     3721 ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat aaaaattggc
     3781 aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac ctgtcccttg
     3841 ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca gagggcaggt
     3901 ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta tggttttggt
     3961 cggcactgcc gacagcctcg cagagcccac ctttacgaag taaagtatag tgtgttatac
     4021 tttacatgga agttgtaccg aaatattgtg ttgtgaagtc tatataattt taaaattatg
     4081 aagtctatat acgtgcgtaa agatat
//
LOCUS       DACCVU010000357         4106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.178, whole genome shotgun sequence.
ACCESSION   DACCVU010000357 DACCVU010000000
VERSION     DACCVU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.178"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003225"
     CDS             44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7840600.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2073..2123
                     /locus_tag="IVW29_003226"
                     /pseudo
     CDS             2073..2123
                     /locus_tag="IVW29_003226"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003227"
     CDS             2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7840601.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
BASE COUNT         1303 a          625 c          828 g         1350 t
ORIGIN      
        1 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
       61 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
      121 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      181 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      241 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      301 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      361 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      421 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      481 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
      541 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
      601 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
      661 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
      721 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc
      781 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
      841 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
      901 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
      961 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
     1021 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
     1081 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
     1141 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
     1201 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1261 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1321 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1381 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1441 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
     1501 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt atggcttata
     1561 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
     1621 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
     1681 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
     1741 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
     1801 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
     1861 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
     1921 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
     1981 tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa
     2041 gtgtaaagga ggaattaatt atgaagtgaa atgaatgtaa cagggttcaa ttaaaagagg
     2101 gaagcgtatc attaacccta taaactacgt ctgccctcat tattggaggg tgaaatgtga
     2161 atacatccta ttcacaatcg aatttacgac acaaccaaat tttaatttgg ctttgcattt
     2221 tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct gatattgcaa
     2281 atgattttaa taaaccacct gcgagtacaa actgggtgaa cacagccttt atgttaacct
     2341 tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc aaaaggttac
     2401 tcctatttgg aattataata aattgtttcg ggtcggtaat tgggtttgtt ggccattctt
     2461 tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct gcatttccag
     2521 cactcgtaat ggttgtagtt gcgcgctata ttccaaagga aaataggggt aaagcatttg
     2581 gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt ggtggaatga
     2641 tagcccatta tattcattgg tcctatcttc tactcattcc tatgataaca attatcactg
     2701 ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat tttgatatca
     2761 aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca acatcatata
     2821 gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa catatcagga
     2881 aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt atgattggag
     2941 ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg gttccttata
     3001 tgatgaaaga tgttcaccag ctaagtactg ccgaaatcgg aagtgtaatt attttccctg
     3061 gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat agaagaggtc
     3121 ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta actgcttcct
     3181 ttcttttaga aacaacatca tggttcatga caattataat cgtatttgtt ttaggtgggc
     3241 tttcgttcac caaaacagtt atatcaacaa ttgtttcaag tagcttgaaa cagcaggaag
     3301 ctggtgctgg aatgagtttg cttaacttta ccagcttttt atcagaggga acaggtattg
     3361 caattgtagg tggtttatta tccataccct tacttgatca aaggttgtta cctatggaag
     3421 ttgatcagtc aacttatctg tatagtaatt tgttattact tttttcagga atcattgtca
     3481 ttagttggct ggttaccttg aatgtatata aacattctca aagggatttc taaatcgtta
     3541 agggatcaac tttgggagag agttcaaaat tgatcctttt tttataacag gaattcaaat
     3601 ctttttgttc cattaaaggg cgcgattgct gaataaaaga tacgagagac ctctcttgta
     3661 tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac aataaaaatt
     3721 ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat aaaaattggc
     3781 aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac ctgtcccttg
     3841 ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca gagggcaggt
     3901 ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta tggttttggt
     3961 cggcactgcc gacagcctcg cagagcccac ctttacgaag taaagtatag tgtgttatac
     4021 tttacatgga agttgtaccg aaatattgtg ttgtgaagtc tatataattt taaaattatg
     4081 aagtctatat acgtgcgtaa agatat
//
LOCUS       DACCVU010000358         4106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.179, whole genome shotgun sequence.
ACCESSION   DACCVU010000358 DACCVU010000000
VERSION     DACCVU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.179"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003228"
     CDS             44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7840602.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2073..2123
                     /locus_tag="IVW29_003229"
                     /pseudo
     CDS             2073..2123
                     /locus_tag="IVW29_003229"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003230"
     CDS             2157..3533
                     /gene="tet(L)"
                     /locus_tag="IVW29_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7840603.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
BASE COUNT         1300 a          626 c          831 g         1349 t
ORIGIN      
        1 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
       61 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
      121 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      181 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      241 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      301 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      361 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      421 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      481 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
      541 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
      601 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
      661 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
      721 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc
      781 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
      841 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
      901 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
      961 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
     1021 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
     1081 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
     1141 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
     1201 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1261 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1321 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1381 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1441 tggatactta aatcaatcgt ttcaaaatgc agttatggag gggatacgct atggctgtga
     1501 acaaggattg tatggttgga atgtgacgga ctgtaaaatc tgttttaagt atggcttata
     1561 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
     1621 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
     1681 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
     1741 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
     1801 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
     1861 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
     1921 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
     1981 tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa
     2041 gtgtaaagga ggaattaatt atgaagtgaa atgaatgtaa cagggttcaa ttaaaagagg
     2101 gaagcgtatc attaacccta taaactacgt ctgccctcat tattggaggg tgaaatgtga
     2161 atacatccta ttcacaatcg aatttacgac acaaccaaat tttaatttgg ctttgcattt
     2221 tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct gatattgcaa
     2281 atgattttaa taaaccacct gcgagtacaa actgggtgaa cacagccttt atgttaacct
     2341 tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc aaaaggttac
     2401 tcctatttgg aattataata aattgtttcg ggtcggtaat tgggtttgtt ggccattctt
     2461 tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct gcatttccag
     2521 cactcgtaat ggttgtagtt gcgcgctata ttccaaagga aaataggggt aaagcatttg
     2581 gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt ggtggaatga
     2641 tagcccatta tattcattgg tcctatcttc tactcattcc tatgataaca attatcactg
     2701 ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat tttgatatca
     2761 aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca acatcatata
     2821 gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa catatcagga
     2881 aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt atgattggag
     2941 ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg gttccttata
     3001 tgatgaaaga tgttcaccag ctaagtactg ccgaaatcgg aagtgtaatt attttccctg
     3061 gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat agaagaggtc
     3121 ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta actgcttcct
     3181 ttcttttaga aacaacatca tggttcatga caattataat cgtatttgtt ttaggtgggc
     3241 tttcgttcac caaaacagtt atatcaacaa ttgtttcaag tagcttgaaa cagcaggaag
     3301 ctggtgctgg aatgagtttg cttaacttta ccagcttttt atcagaggga acaggtattg
     3361 caattgtagg tggtttatta tccataccct tacttgatca aaggttgtta cctatggaag
     3421 ttgatcagtc aacttatctg tatagtaatt tgttattact tttttcagga atcattgtca
     3481 ttagttggct ggttaccttg aatgtatata aacattctca aagggatttc taaatcgtta
     3541 agggatcaac tttgggagag agttcaaaat tgatcctttt tttataacag gaattcaaat
     3601 ctttttgttc cattaaaggg cgcgattgct gaataaaaga tacgagagac ctctcttgta
     3661 tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac aataaaaatt
     3721 ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat aaaaattggc
     3781 aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac ctgtcccttg
     3841 ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca gagggcaggt
     3901 ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta tggttttggt
     3961 cggcactgcc gacagcctcg cagagcccac ctttacgaag taaagtatag tgtgttatac
     4021 tttacatgga agttgtaccg aaatattgtg ttgtgaagtc tatataattt taaaattatg
     4081 aagtctatat acgtgcgtaa agatat
//
LOCUS       DACCVU010000359         3447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.197, whole genome shotgun sequence.
ACCESSION   DACCVU010000359 DACCVU010000000
VERSION     DACCVU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.197"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..336
                     /locus_tag="IVW29_003231"
     CDS             <1..336
                     /locus_tag="IVW29_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7840604.1"
                     /translation="NQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLA
                     AMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRL
                     APEKITMFE"
     gene            690..2168
                     /gene="msr"
                     /locus_tag="IVW29_003232"
     CDS             690..2168
                     /gene="msr"
                     /locus_tag="IVW29_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7840605.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2215..2661
                     /locus_tag="IVW29_003233"
     CDS             2215..2661
                     /locus_tag="IVW29_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7840606.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2860..3375)
                     /locus_tag="IVW29_003234"
     CDS             complement(2860..3375)
                     /locus_tag="IVW29_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7840607.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1098 a          580 c          719 g         1050 t
ORIGIN      
        1 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
       61 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
      121 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
      181 ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
      241 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
      301 cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta ctattcagta
      361 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
      421 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
      481 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
      541 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
      601 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
      661 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
      721 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
      781 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
      841 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
      901 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
      961 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     1021 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     1081 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     1141 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     1201 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     1261 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     1321 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     1381 gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa caaaaagaca
     1441 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     1501 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     1561 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     1621 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     1681 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     1741 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     1801 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     1861 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     1921 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     1981 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     2041 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     2101 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     2161 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     2221 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     2281 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     2341 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     2401 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     2461 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     2521 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     2581 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     2641 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt
     2701 ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc
     2761 ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg
     2821 ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat
     2881 ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg
     2941 gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt
     3001 ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc
     3061 tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt
     3121 aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa
     3181 gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg
     3241 gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat
     3301 cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata
     3361 ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa
     3421 agtttccacg ttttacatgt gcatatt
//
LOCUS       DACCVU010000360         3447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.198, whole genome shotgun sequence.
ACCESSION   DACCVU010000360 DACCVU010000000
VERSION     DACCVU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.198"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..336
                     /locus_tag="IVW29_003235"
     CDS             <1..336
                     /locus_tag="IVW29_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7840608.1"
                     /translation="NQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLA
                     AMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRL
                     APEKITMFE"
     gene            690..2168
                     /gene="msr"
                     /locus_tag="IVW29_003236"
     CDS             690..2168
                     /gene="msr"
                     /locus_tag="IVW29_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7840609.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2215..2661
                     /locus_tag="IVW29_003237"
     CDS             2215..2661
                     /locus_tag="IVW29_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7840610.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2860..3375)
                     /locus_tag="IVW29_003238"
     CDS             complement(2860..3375)
                     /locus_tag="IVW29_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7840611.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1098 a          579 c          719 g         1051 t
ORIGIN      
        1 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
       61 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
      121 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
      181 ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
      241 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
      301 cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta ctattcagta
      361 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
      421 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
      481 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
      541 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
      601 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
      661 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
      721 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
      781 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
      841 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
      901 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
      961 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     1021 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     1081 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     1141 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     1201 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     1261 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     1321 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     1381 gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc ttcctctaaa caaaaagaca
     1441 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     1501 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     1561 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     1621 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     1681 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     1741 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     1801 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     1861 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     1921 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     1981 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     2041 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     2101 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     2161 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     2221 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     2281 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     2341 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     2401 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     2461 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     2521 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     2581 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     2641 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt
     2701 ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc
     2761 ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg
     2821 ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat
     2881 ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg
     2941 gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt
     3001 ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc
     3061 tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt
     3121 aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa
     3181 gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg
     3241 gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat
     3301 cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata
     3361 ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa
     3421 agtttccacg ttttacatgt gcatatt
//
LOCUS       DACCVU010000361         3277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.199, whole genome shotgun sequence.
ACCESSION   DACCVU010000361 DACCVU010000000
VERSION     DACCVU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.199"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            275..772
                     /gene="dfrG"
                     /locus_tag="IVW29_003239"
     CDS             275..772
                     /gene="dfrG"
                     /locus_tag="IVW29_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7840612.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(844..2796)
                     /locus_tag="IVW29_003240"
     CDS             complement(844..2796)
                     /locus_tag="IVW29_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7840613.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2803..3099)
                     /locus_tag="IVW29_003241"
     CDS             complement(2803..3099)
                     /locus_tag="IVW29_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7840614.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1016 a          566 c          538 g         1157 t
ORIGIN      
        1 taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt
       61 tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg
      121 ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat
      181 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
      241 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      301 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      361 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      421 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      481 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      541 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      601 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      661 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      721 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      781 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      841 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      901 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      961 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
     1021 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
     1081 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
     1141 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1201 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1261 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1321 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1381 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1441 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1501 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1561 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1621 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1681 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1741 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1801 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1861 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1921 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1981 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     2041 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     2101 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     2161 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2221 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2281 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2341 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2401 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2461 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2521 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2581 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2641 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2701 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2761 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2821 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2881 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2941 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     3001 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     3061 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     3121 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3181 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3241 attttcactt tttgtactcc aaaactgaac tacttaa
//
LOCUS       DACCVU010000362         1988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.244, whole genome shotgun sequence.
ACCESSION   DACCVU010000362 DACCVU010000000
VERSION     DACCVU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.244"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003242"
     CDS             44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7840615.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          678 a          308 c          415 g          587 t
ORIGIN      
        1 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
       61 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
      121 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      181 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      241 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      301 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      361 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      421 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      481 gaaactttct atggaaatta taatcaaaca gaaagtagag ctgcacccta atatgtgtgt
      541 gatgagctgt acggaacctg agcaatggga tgtggtaata gaaggaaatg atgacctttt
      601 agagaaatat atgtccggta aatcattaga agcattagaa ctcgaacaag aggaaatcag
      661 aagatttcag aattgctcct tgtaccctgt ttatcatgga agcgcaaaaa gcaacatagg
      721 gattgagcag cttatagaag tgataacgaa taaattttat tcatcaacat acagaaagaa
      781 gtctgaactt tgcggaaatg tcttcaaaat tgaatattcg gaagaaagac aacgtcttgc
      841 atatgtacgc ctttatggcg gaatcctgca tttgcgggat tcggttagaa tatcggaaaa
      901 ggaaaaaata aaaattacag aaatgtatac ttcaataaat ggtgaattat gtaaaattga
      961 taaggcttat tccggggaaa ttgttatttt gcaaaatgag tttttgaagc taaatagtgt
     1021 tcttggagat acaaagctat tgccacagag agagagaatt gaaaatccgc tccctctgct
     1081 gcaaacaact gttgaaccga gcaaacctca acaaagggaa atgttacttg atgcactttt
     1141 agaaatctcc gacagtgacc cgcttctaca atattatgtg gattctacga cacatgaaat
     1201 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1261 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1321 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1381 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1441 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
     1501 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata
     1561 ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg tactggagca
     1621 agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta aagtttatgc
     1681 accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg caaatatcgt
     1741 aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg ctcgatgtat
     1801 tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt gtttagcaga
     1861 gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc gtctaaatag
     1921 tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt tatgttgtta
     1981 tataaata
//
LOCUS       DACCVU010000363         1988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.245, whole genome shotgun sequence.
ACCESSION   DACCVU010000363 DACCVU010000000
VERSION     DACCVU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.245"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003243"
     CDS             44..1963
                     /gene="tet(M)"
                     /locus_tag="IVW29_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7840616.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          683 a          303 c          401 g          601 t
ORIGIN      
        1 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
       61 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
      121 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      181 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      241 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      301 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      361 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      421 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      481 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
      541 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
      601 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
      661 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
      721 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc
      781 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
      841 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
      901 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
      961 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
     1021 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
     1081 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
     1141 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
     1201 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1261 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1321 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1381 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1441 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
     1501 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata
     1561 ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg tactggagca
     1621 agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta aagtttatgc
     1681 accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg caaatatcgt
     1741 aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg ctcgatgtat
     1801 tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt gtttagcaga
     1861 gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc gtctaaatag
     1921 tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt tatgttgtta
     1981 tataaata
//
LOCUS       DACCVU010000364         1912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.251, whole genome shotgun sequence.
ACCESSION   DACCVU010000364 DACCVU010000000
VERSION     DACCVU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.251"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..997
                     /locus_tag="IVW29_003244"
     CDS             <1..997
                     /locus_tag="IVW29_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7840617.1"
                     /translation="KENYSNRQVADVLGCVPQTINNEIKRGTVTQLNRQNQNGKVYDY
                     YHDVYDADAGQAAYEKNRLNCGRRPLWADTDAFIEWADDMMLLEKWSPDVVVGFARKH
                     ELFDPAIIPGTTTLYQWIDRGIMKTKNMDLLEKLSRKPKSSRHGNRPNKRVLGKSIEE
                     RPEAVGTRKTFGHWEVDTVVGNKTKSDAVLLTLVERQTRFEVILKLAGKDAESVNQAV
                     RQLRERAGAYFGQLFKTVTSDNGSEFAGLHDALQDVANIYFSHPYASWERGTSENQHK
                     LIRRFIPKGSPIGNVSENQILRIQRWMNDYPRKILGYATPHDTFVQAFRQERLTA"
     gene            1299..1847
                     /gene="aac(6')"
                     /locus_tag="IVW29_003245"
     CDS             1299..1847
                     /gene="aac(6')"
                     /locus_tag="IVW29_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7840618.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          568 a          429 c          509 g          406 t
ORIGIN      
        1 aaaggaaaac tattccaacc gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat
       61 aaacaacgaa atcaaacgcg ggaccgttac tcagcttaac cgccagaatc agaacgggaa
      121 ggtctacgac tattatcatg acgtctacga cgccgatgcc gggcaggcgg cctacgagaa
      181 gaaccgcttg aactgcggca gacggccctt atgggccgac acggacgcct tcattgaatg
      241 ggcggacgac atgatgctgt tggaaaaatg gtccccggac gtggtggtgg gctttgccag
      301 aaaacacgaa ttgttcgacc cggccattat ccctgggacc accaccctct atcagtggat
      361 cgaccgtggc atcatgaaaa cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc
      421 gaaatcgtcc cgtcatggca accgcccgaa taaacgggtg ctagggaaat ccattgaaga
      481 gcgcccggag gcggttggta cccggaaaac cttcggccat tgggaagtcg acacggtcgt
      541 aggaaacaag accaagtcgg atgccgtgct gctgacgttg gtggaacggc agacacgctt
      601 cgaggtcatt ctgaagctgg ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca
      661 gctgagagaa cgggctggtg cgtacttcgg ccagctgttc aagacggtta cctcggacaa
      721 cggctcggag ttcgcgggac tgcacgatgc cttgcaggat gtggcaaaca tctacttcag
      781 ccacccctat gcttcctggg agcgcgggac gagcgaaaac caacacaagc tgattcgccg
      841 tttcatcccc aagggcagcc caatcggcaa cgtgagcgaa aaccaaatct tgcggattca
      901 gcgttggatg aacgattacc cgcgaaagat tttgggctat gcaacgccgc atgacacgtt
      961 cgtccaggca tttaggcagg aacggttgac ggcttgacac gtgggccctt gacaacgagc
     1021 ctccctcggc atggcttgtg tctggaaagc tggccggggc cagccagtcg ggagactacc
     1081 atgcctcagc tcattgtcaa gggcccactc gattagaaaa attagacggt tggtggtaac
     1141 gagcaaccgg gtcaccatgg gtggctaact taaacttgaa atttacgata aataatgaaa
     1201 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     1261 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     1321 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     1381 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     1441 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     1501 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     1561 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     1621 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     1681 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     1741 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     1801 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     1861 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
LOCUS       DACCVU010000365         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.274, whole genome shotgun sequence.
ACCESSION   DACCVU010000365 DACCVU010000000
VERSION     DACCVU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.274"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            45..296
                     /locus_tag="IVW29_003246"
                     /pseudo
     CDS             45..296
                     /locus_tag="IVW29_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1183
                     /locus_tag="IVW29_003247"
                     /note="aph(3')-IIIa"
     CDS             389..1183
                     /locus_tag="IVW29_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7840619.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          233 c          322 g          369 t
ORIGIN      
        1 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
       61 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      121 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      181 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      241 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      301 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      361 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      421 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      481 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      541 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      601 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      661 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      721 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      781 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      841 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      901 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      961 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1021 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1081 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1141 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1201 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1261 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1321 gcctacgagg aatttgtatc gataagaa
//
LOCUS       DACCVU010000366         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.293, whole genome shotgun sequence.
ACCESSION   DACCVU010000366 DACCVU010000000
VERSION     DACCVU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.293"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            228..1103
                     /locus_tag="IVW29_003248"
                     /note="aph(2'')-Ia"
     CDS             228..1103
                     /locus_tag="IVW29_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7840620.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          517 a           86 c          196 g          389 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaa
//
LOCUS       DACCVU010000367          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.315, whole genome shotgun sequence.
ACCESSION   DACCVU010000367 DACCVU010000000
VERSION     DACCVU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.315"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..738
                     /gene="erm(B)"
                     /locus_tag="IVW29_003249"
     CDS             1..738
                     /gene="erm(B)"
                     /locus_tag="IVW29_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7840621.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          344 a          154 c          137 g          268 t
ORIGIN      
        1 atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa
       61 ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa
      121 gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac
      181 agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt
      241 caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat
      301 attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct
      361 gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca
      421 ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa
      481 tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca
      541 gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga
      601 gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta
      661 aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta
      721 tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac
      781 taaagggaat gtagataaat tattaggtat agggcacctc taataactac caattaattc
      841 acctcaaaat gaaattagaa aaaaacagcg aaatttttaa atctatttct tatcgataca
      901 aat
//
LOCUS       DACCVU010000368          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16372
            SAMN14604049-rid9903053.guided.316, whole genome shotgun sequence.
ACCESSION   DACCVU010000368 DACCVU010000000
VERSION     DACCVU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604049
            Sequence Read Archive: SRR11551427
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744163.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744163.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,108
            CDSs (with protein)               :: 3,108
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 41 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604049-rid9903053.guided.316"
                     /isolate="UW16372"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Schleswig-Holstein"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..738
                     /gene="erm(B)"
                     /locus_tag="IVW29_003250"
     CDS             1..738
                     /gene="erm(B)"
                     /locus_tag="IVW29_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7840622.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          344 a          154 c          137 g          268 t
ORIGIN      
        1 atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa
       61 ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa
      121 gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac
      181 agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt
      241 caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggagt
      301 attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct
      361 gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca
      421 ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa
      481 tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca
      541 gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga
      601 gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta
      661 aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta
      721 tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac
      781 taaagggaat gtagataaat tattaggtat agggcacctc taataactac caattaattc
      841 acctcaaaat gaaattagaa aaaaacagcg aaatttttaa atctatttct tatcgataca
      901 aat
//
